AT1G28140

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013259
Physical Location & Seq
Reverse (-)
9831876 .. 9834510
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28140.1

Sequence Viewer

Length: 843 bp
ATGGCCTCGCGTTTGCTATCGACGACGACACTCGTCACACCGCCGGCTTATTTCAACAAATCGCCGGCGTTTTTAACGGCCAGAGTTGGGGTGAGAAGAGGAAGGGCTAACGTTAAGGCTGTGAGCAATAGTTCGCAAGGAGCCGTCGATGGAACCGTTTACAAAGGTGTTTACGGTCCTTGGACCATTGACCAAGCCGATGTCAAAGAGGTTATTTTGTACAGATCAGGACTAGTAACTGCTGCTGCATCTTTTGTAGCTGCTTCTTCTGCTGCCTTTTTACCAGGAGACTCTTGGTTAAGTGAAACAATCAAGCAAAACCATGATCTGTTTTACTTTGTTGGTGCTAGTGGTTTGGGGCTATCTCTGTTTCTAATACATATATATGTTACGGAGATTAAGAGGACTCTTCAAGCTTTATGGGCACTTGGTTTTGTTGGTTCTTTCGCTACTTACGCAGCCCTTGCTAGACCAGCTGGTGATAACTTGGTTCATTATGTCGTTGATCATCCATCCGCTGTTTGGTTTGTTGGTCCCCTATTCGCATCTCTCACAGGACTCGTTTTCAAAGAAGGTCTTTGTTATGGAAAGCTAGAAGCTGGCCTTCTTACATTCATCATACCTTCAGTTCTTCTTGGGCATTTGAGTGGTCTGATGAATGATGAGGTAAAACTGGTGTTGCTAGGAACATGGATGGCTCTCTTTCTAGTATTTGCTGGAAGAAAGTTTACTCAGCCTATTAAGGATGATATCGGAGATAAATCTGTTTTCACGTTCATGTCCCTTTCTGATGATGAAAAGAAGGCTATAGTTGAAAAGCTCGAGCAAGAAAAGTTGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

280

Amino Acids

30.28

Weight (kDa)

8.52

Isoelectric Point (pI)

30.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 121 - 259 3e-55 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 10
AciI CCGC 2 cut(s) 41, 516
AclI AACGTT 1 cut(s) 111
AcoI YGGCCR 1 cut(s) 78
AcuI CTGAAG 1 cut(s) 609
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 87, 522
AgsI TTSAA 4 cut(s) 55, 413, 568, 815
AhlI ACTAGT 1 cut(s) 232
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 6 cut(s) 260, 416, 476, 592, 599, 820
AluI AGCT 6 cut(s) 260, 416, 476, 592, 599, 820
Alw26I GTCTC 1 cut(s) 282
Ama87I CYCGRG 1 cut(s) 821
AoxI GGCC 3 cut(s) 3, 78, 601
ApeKI GCWGC 5 cut(s) 242, 245, 260, 272, 458
AspS9I GGNCC 3 cut(s) 176, 183, 533
AsuHPI GGTGA 2 cut(s) 103, 491
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 3 cut(s) 176, 183, 533
BaeGI GKGCMC 1 cut(s) 427
BarI GAAGNNNNNNTAC 2 cut(s) 712, 744
BbvI GCAGC 5 cut(s) 229, 232, 247, 259, 470
BccI CCATC 3 cut(s) 143, 520, 688
BceAI ACGGC 2 cut(s) 93, 128
BciT130I CCWGG 1 cut(s) 285
BclI TGATCA 1 cut(s) 505
BcoDI GTCTC 1 cut(s) 282
BcuI ACTAGT 1 cut(s) 232
BfaI CTAG 6 cut(s) 233, 348, 468, 593, 683, 707
BfmI CTRYAG 1 cut(s) 807
BisI GCNGC 5 cut(s) 243, 246, 261, 273, 459
BlsI GCNGC 5 cut(s) 244, 247, 262, 274, 460
Bme1390I CCNGG 1 cut(s) 285
Bme18I GGWCC 3 cut(s) 176, 183, 533
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 3 cut(s) 176, 183, 533
BmiI GGNNCC 3 cut(s) 142, 154, 535
BmrFI CCNGG 1 cut(s) 285
BmsI GCATC 2 cut(s) 257, 554
BoxI GACNNNNGTC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 179
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 522
Bse118I RCCGGY 2 cut(s) 43, 64
Bse1I ACTGG 1 cut(s) 678
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 4 cut(s) 508, 512, 699, 751
BseLI CCNNNNNNNGG 2 cut(s) 87, 522
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 1 cut(s) 678
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 5 cut(s) 229, 232, 247, 259, 470
Bsh1236I CGCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 5, 80, 603
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 2 cut(s) 44, 65
BslFI GGGAC 2 cut(s) 519, 766
BslI CCNNNNNNNGG 2 cut(s) 87, 522
BsmAI GTCTC 1 cut(s) 282
BsmFI GGGAC 2 cut(s) 519, 766
BsnI GGCC 3 cut(s) 5, 80, 603
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 1 cut(s) 427
Bsp1407I TGTACA 1 cut(s) 219
Bsp143I GATC 3 cut(s) 224, 325, 505
BspACI CCGC 2 cut(s) 41, 516
BspANI GGCC 3 cut(s) 5, 80, 603
BspCNI CTCAG 1 cut(s) 745
BspFNI CGCG 1 cut(s) 10
BspLI GGNNCC 3 cut(s) 142, 154, 535
BsrFI RCCGGY 2 cut(s) 43, 64
BsrGI TGTACA 1 cut(s) 219
BsrI ACTGG 1 cut(s) 678
BssAI RCCGGY 2 cut(s) 43, 64
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 3 cut(s) 224, 325, 505
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 157, 176
Bst6I CTCTTC 2 cut(s) 91, 414
BstAPI GCANNNNNTGC 1 cut(s) 464
BstAUI TGTACA 1 cut(s) 219
BstC8I GCNNGC 3 cut(s) 45, 66, 601
BstDEI CTNAG 1 cut(s) 732
BstF5I GGATG 4 cut(s) 508, 512, 699, 751
BstFNI CGCG 1 cut(s) 10
BstKTI GATC 3 cut(s) 227, 328, 508
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 3 cut(s) 224, 325, 505
BstMWI GCNNNNNNNGC 5 cut(s) 269, 422, 455, 464, 473
BstNI CCWGG 1 cut(s) 285
BstPAI GACNNNNGTC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 283
BstSFI CTRYAG 1 cut(s) 807
BstSLI GKGCMC 1 cut(s) 427
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 5 cut(s) 229, 232, 247, 259, 470
BsuRI GGCC 3 cut(s) 5, 80, 603
BtsCI GGATG 4 cut(s) 508, 512, 699, 751
Cac8I GCNNGC 3 cut(s) 45, 66, 601
Cfr10I RCCGGY 2 cut(s) 43, 64
Cfr13I GGNCC 3 cut(s) 176, 183, 533
Csp6I GTAC 1 cut(s) 220
CviAII CATG 3 cut(s) 323, 690, 778
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 1 cut(s) 732
DpnI GATC 3 cut(s) 226, 327, 507
DpnII GATC 3 cut(s) 224, 325, 505
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 91, 414
EarI CTCTTC 2 cut(s) 91, 414
Eco130I CCWWGG 1 cut(s) 179
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 3 cut(s) 176, 183, 533
Eco57I CTGAAG 1 cut(s) 609
Eco88I CYCGRG 1 cut(s) 821
EcoRII CCWGG 1 cut(s) 283
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 3 cut(s) 326, 693, 781
FalI AAGNNNNNCTT 4 cut(s) 561, 593, 588, 620
FaqI GGGAC 2 cut(s) 519, 766
FatI CATG 3 cut(s) 322, 689, 777
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 5 cut(s) 243, 246, 261, 273, 459
FokI GGATG 4 cut(s) 495, 499, 706, 758
Fsp4HI GCNGC 5 cut(s) 243, 246, 261, 273, 459
FspBI CTAG 6 cut(s) 233, 348, 468, 593, 683, 707
GluI GCNGC 5 cut(s) 243, 246, 261, 273, 459
HaeIII GGCC 3 cut(s) 5, 80, 603
HapII CCGG 2 cut(s) 44, 65
Hin1II CATG 3 cut(s) 326, 693, 781
HindIII AAGCTT 1 cut(s) 414
HinfI GANTC 3 cut(s) 290, 406, 558
HpaII CCGG 2 cut(s) 44, 65
HphI GGTGA 2 cut(s) 103, 491
Hpy166II GTNNAC 3 cut(s) 160, 172, 729
Hpy188I TCNGA 3 cut(s) 654, 755, 790
Hpy188III TCNNGA 1 cut(s) 228
Hpy8I GTNNAC 3 cut(s) 160, 172, 729
Hpy99I CGWCG 3 cut(s) 25, 28, 149
HpyAV CCTTC 5 cut(s) 96, 566, 614, 633, 796
HpyCH4III ACNGT 2 cut(s) 157, 176
HpyCH4IV ACGT 2 cut(s) 111, 773
HpyCH4V TGCA 1 cut(s) 248
HpyF10VI GCNNNNNNNGC 5 cut(s) 269, 422, 455, 464, 473
HpyF3I CTNAG 1 cut(s) 732
HpySE526I ACGT 2 cut(s) 111, 773
Hsp92II CATG 3 cut(s) 326, 693, 781
KroI GCCGGC 2 cut(s) 43, 64
KroNI GCCGGC 2 cut(s) 45, 66
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 3 cut(s) 224, 325, 505
LmnI GCTCC 1 cut(s) 140
Lsp1109I GCAGC 5 cut(s) 229, 232, 247, 259, 470
LweI GCATC 2 cut(s) 257, 554
MaeI CTAG 6 cut(s) 233, 348, 468, 593, 683, 707
MaeII ACGT 2 cut(s) 111, 773
MaeIII GTNAC 3 cut(s) 34, 235, 388
MalI GATC 3 cut(s) 226, 327, 507
MboI GATC 3 cut(s) 224, 325, 505
MboII GAAGA 5 cut(s) 108, 258, 401, 623, 732
MhlI GDGCHC 1 cut(s) 427
MlyI GAGTC 3 cut(s) 284, 400, 552
MnlI CCTC 5 cut(s) 16, 92, 202, 396, 658
MreI CGCCGGCG 1 cut(s) 64
MroNI GCCGGC 2 cut(s) 43, 64
MseI TTAA 5 cut(s) 74, 114, 299, 399, 741
MslI CAYNNNNRTG 3 cut(s) 384, 645, 776
MspA1I CMGCKG 2 cut(s) 476, 518
MspI CCGG 2 cut(s) 44, 65
MspR9I CCNGG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 285
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 5 cut(s) 269, 422, 455, 464, 473
NaeI GCCGGC 2 cut(s) 45, 66
NdeII GATC 3 cut(s) 224, 325, 505
NgoMIV GCCGGC 2 cut(s) 43, 64
NlaIII CATG 3 cut(s) 326, 693, 781
NlaIV GGNNCC 3 cut(s) 142, 154, 535
NmuCI GTSAC 1 cut(s) 34
PaeR7I CTCGAG 1 cut(s) 821
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdiI GCCGGC 2 cut(s) 45, 66
PkrI GCNGC 5 cut(s) 244, 247, 262, 274, 460
PleI GAGTC 3 cut(s) 284, 400, 552
PpsI GAGTC 3 cut(s) 284, 400, 552
PshAI GACNNNNGTC 1 cut(s) 32
Psp1406I AACGTT 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 3 cut(s) 142, 154, 535
PspPI GGNCC 3 cut(s) 176, 183, 533
PspXI VCTCGAGB 1 cut(s) 821
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
RseI CAYNNNNRTG 3 cut(s) 384, 645, 776
SaqAI TTAA 5 cut(s) 74, 114, 299, 399, 741
SatI GCNGC 5 cut(s) 243, 246, 261, 273, 459
Sau3AI GATC 3 cut(s) 224, 325, 505
Sau96I GGNCC 3 cut(s) 176, 183, 533
SchI GAGTC 3 cut(s) 284, 400, 552
ScrFI CCNGG 1 cut(s) 285
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 2 cut(s) 257, 554
SfcI CTRYAG 1 cut(s) 807
Sfr274I CTCGAG 1 cut(s) 821
SgrAI CRCCGGYG 1 cut(s) 64
SinI GGWCC 3 cut(s) 176, 183, 533
SlaI CTCGAG 1 cut(s) 821
SmiMI CAYNNNNRTG 3 cut(s) 384, 645, 776
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
SpeI ACTAGT 1 cut(s) 232
SsiI CCGC 2 cut(s) 41, 516
SspMI CTAG 6 cut(s) 233, 348, 468, 593, 683, 707
StyD4I CCNGG 1 cut(s) 283
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 2 cut(s) 157, 176
TaiI ACGT 2 cut(s) 114, 776
TaqI TCGA 3 cut(s) 20, 147, 822
TatI WGTACW 1 cut(s) 219
Tru1I TTAA 5 cut(s) 74, 114, 299, 399, 741
Tru9I TTAA 5 cut(s) 74, 114, 299, 399, 741
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 5 cut(s) 242, 245, 260, 272, 458
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 5 cut(s) 482, 604, 671, 766, 810
TspGWI ACGGA 1 cut(s) 407
VpaK11BI GGWCC 3 cut(s) 176, 183, 533
XcmI CCANNNNNNNNNTGG 2 cut(s) 291, 519
XhoI CTCGAG 1 cut(s) 821
XspI CTAG 6 cut(s) 233, 348, 468, 593, 683, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.