FvH4_4g22640

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013259
Physical Location & Seq
Reverse (-)
25375461 .. 25377023
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22640.t3

Sequence Viewer

Length: 858 bp
ATGGCTTCTCATATGCTTTACGCATCCATCATCACTTCTCATCCTTCATCAAACCTCACCAAGCTTCCAAGGCCTTCTTCTTCTTCATCACCCAGACTTCCAACTCGACTCAAGTCTTTCAAGTGCAAGGCTCAAAGCTCAAGCTCTCAGTCCCAGACTGTCTATCAGGGCATCTATGGCCCTTGGTCTGTTGACTCCACTGACGTTAAAGAGGTCATTCTGTACAGGTCAGGATTAGTGACTGCTGCTACATCATTTGTAATTGCAGCTTCTACTGCCTTTTTGCCTGACAGTTTCTTAGCTAACGAGGTTATTAGGCCTAATCTTGATTACTTATATGCAATTGGAGCCGGTGGGTTGGGTTTGTCATTGGTTCTGATTCACATTTATGTGACTGAGATTAAGCGCACTTTGCAAGCATTTTGGGGGCTTGGTGTTGTTGGGTCTTTGGCTGCGTATATATGCTTGGCTCAACCAGCTGGAGAGGGCTTGGTTCAGTATGTGGTTGATAACCCCACTGCTGTTTGGTTTGTCGGTCCTTTGTTTGCAGCCCTCACAGGACTGGTTTTCAAGGAAGGTCTTTGCTATGGAAAGTTGGAAGCTGGACTACTCACATTCATAATACCTATTGTTCTTCTTGGGCACCTGAGTGGCTTGATGGATGATGGAGTGAAACTTTCTCTGCTAGGCTCATGGATGGCCCTGTTTGTATTATTTGCCGGAAGGAAGTTCACACAACCCATTAAGGATGACATTGGTGACAAGTCTGTCTTCATATTCCAATCTTTGCCTGAAGATGAGAAGAAGGCCTTGGTTAAGAAACTTGAGCAACAGGAGTATAGTCAGAATCCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

286

Amino Acids

30.87

Weight (kDa)

8.32

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 122 - 249 1e-45 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 767
AccB1I GGYRCC 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 813
AfaI GTAC 1 cut(s) 224
AgsI TTSAA 2 cut(s) 121, 571
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 794, 826
AleI CACNNNNGTG 1 cut(s) 648
AluBI AGCT 7 cut(s) 64, 138, 144, 269, 302, 479, 602
AluI AGCT 7 cut(s) 64, 138, 144, 269, 302, 479, 602
AoxI GGCC 5 cut(s) 71, 178, 317, 699, 807
ApeKI GCWGC 4 cut(s) 245, 266, 452, 548
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 3 cut(s) 179, 536, 700
AsuHPI GGTGA 3 cut(s) 49, 81, 770
AvaII GGWCC 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 645
BanI GGYRCC 1 cut(s) 642
BarI GAAGNNNNNNTAC 2 cut(s) 591, 623
BbsI GAAGAC 1 cut(s) 763
BbvI GCAGC 4 cut(s) 232, 278, 439, 560
BccI CCATC 4 cut(s) 35, 652, 659, 691
BfaI CTAG 1 cut(s) 686
BisI GCNGC 4 cut(s) 246, 267, 453, 549
BlsI GCNGC 4 cut(s) 247, 268, 454, 550
Bme18I GGWCC 1 cut(s) 536
BmgT120I GGNCC 3 cut(s) 179, 536, 700
BmiI GGNNCC 2 cut(s) 349, 644
BmsI GCATC 2 cut(s) 32, 180
BoxI GACNNNNGTC 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 763
BpmI CTGGAG 1 cut(s) 501
BpuEI CTTGAG 3 cut(s) 95, 124, 845
BsaJI CCNNGG 3 cut(s) 68, 182, 810
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 1 cut(s) 567
BseDI CCNNGG 3 cut(s) 68, 182, 810
BseGI GGATG 5 cut(s) 23, 40, 667, 702, 754
BseMII CTCAG 3 cut(s) 161, 387, 638
BseNI ACTGG 1 cut(s) 567
BseSI GKGCMC 1 cut(s) 645
BseXI GCAGC 4 cut(s) 232, 278, 439, 560
BshFI GGCC 5 cut(s) 73, 180, 319, 701, 809
BshNI GGYRCC 1 cut(s) 642
BsiSI CCGG 2 cut(s) 351, 720
BslFI GGGAC 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 136
BsnI GGCC 5 cut(s) 73, 180, 319, 701, 809
Bsp1286I GDGCHC 1 cut(s) 645
Bsp1407I TGTACA 1 cut(s) 222
BspANI GGCC 5 cut(s) 73, 180, 319, 701, 809
BspCNI CTCAG 3 cut(s) 160, 388, 639
BspLI GGNNCC 2 cut(s) 349, 644
BspT107I GGYRCC 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 1 cut(s) 567
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 3 cut(s) 68, 182, 810
BssT1I CCWWGG 3 cut(s) 68, 182, 810
Bst4CI ACNGT 2 cut(s) 160, 293
BstAUI TGTACA 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 4 cut(s) 147, 298, 396, 647
BstF5I GGATG 5 cut(s) 23, 40, 667, 702, 754
BstHHI GCGC 1 cut(s) 408
BstMWI GCNNNNNNNGC 6 cut(s) 70, 177, 275, 347, 412, 476
BstPAI GACNNNNGTC 1 cut(s) 112
BstSLI GKGCMC 1 cut(s) 645
BstV1I GCAGC 4 cut(s) 232, 278, 439, 560
BstV2I GAAGAC 1 cut(s) 763
BsuRI GGCC 5 cut(s) 73, 180, 319, 701, 809
BtsCI GGATG 5 cut(s) 23, 40, 667, 702, 754
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 2 cut(s) 198, 516
Cac8I GCNNGC 1 cut(s) 417
CfoI GCGC 1 cut(s) 408
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 3 cut(s) 179, 536, 700
Csp6I GTAC 1 cut(s) 223
CviAII CATG 1 cut(s) 693
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 4 cut(s) 147, 298, 396, 647
DrdI GACNNNNNNGTC 1 cut(s) 767
DseDI GACNNNNNNGTC 1 cut(s) 767
Eco130I CCWWGG 3 cut(s) 68, 182, 810
Eco147I AGGCCT 3 cut(s) 73, 319, 809
Eco47I GGWCC 1 cut(s) 536
Eco57I CTGAAG 1 cut(s) 813
EcoT14I CCWWGG 3 cut(s) 68, 182, 810
ErhI CCWWGG 3 cut(s) 68, 182, 810
FaeI CATG 1 cut(s) 696
FalI AAGNNNNNCTT 6 cut(s) 61, 93, 755, 787, 794, 826
FaqI GGGAC 1 cut(s) 136
FatI CATG 1 cut(s) 692
FauNDI CATATG 1 cut(s) 12
Fnu4HI GCNGC 4 cut(s) 246, 267, 453, 549
FokI GGATG 5 cut(s) 10, 27, 674, 709, 761
Fsp4HI GCNGC 4 cut(s) 246, 267, 453, 549
FspBI CTAG 1 cut(s) 686
GlaI GCGC 1 cut(s) 407
GluI GCNGC 4 cut(s) 246, 267, 453, 549
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 5 cut(s) 73, 180, 319, 701, 809
HapII CCGG 2 cut(s) 351, 720
HhaI GCGC 1 cut(s) 408
Hin1II CATG 1 cut(s) 696
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 4 cut(s) 108, 194, 379, 847
HpaII CCGG 2 cut(s) 351, 720
HphI GGTGA 3 cut(s) 49, 81, 770
Hpy166II GTNNAC 2 cut(s) 193, 732
Hpy188I TCNGA 2 cut(s) 378, 846
Hpy188III TCNNGA 2 cut(s) 231, 326
Hpy8I GTNNAC 2 cut(s) 193, 732
HpyAV CCTTC 5 cut(s) 54, 84, 569, 717, 799
HpyCH4III ACNGT 2 cut(s) 160, 293
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 5 cut(s) 126, 266, 341, 415, 548
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 177, 275, 347, 412, 476
HpyF3I CTNAG 4 cut(s) 147, 298, 396, 647
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 1 cut(s) 696
HspAI GCGC 1 cut(s) 406
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 4 cut(s) 232, 278, 439, 560
LweI GCATC 2 cut(s) 32, 180
MaeI CTAG 1 cut(s) 686
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 3 cut(s) 238, 391, 758
MboII GAAGA 7 cut(s) 69, 72, 75, 626, 763, 806, 814
MfeI CAATTG 1 cut(s) 342
MhlI GDGCHC 1 cut(s) 645
MluCI AATT 3 cut(s) 261, 342, 853
MlyI GAGTC 2 cut(s) 102, 188
MmeI TCCRAC 2 cut(s) 125, 576
MnlI CCTC 5 cut(s) 65, 205, 301, 478, 563
MseI TTAA 4 cut(s) 207, 402, 744, 816
MslI CAYNNNNRTG 3 cut(s) 387, 389, 648
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 2 cut(s) 351, 720
MunI CAATTG 1 cut(s) 342
MwoI GCNNNNNNNGC 6 cut(s) 70, 177, 275, 347, 412, 476
NdeI CATATG 1 cut(s) 12
NlaIII CATG 1 cut(s) 696
NlaIV GGNNCC 2 cut(s) 349, 644
NmuCI GTSAC 3 cut(s) 238, 391, 758
OliI CACNNNNGTG 1 cut(s) 648
PceI AGGCCT 3 cut(s) 73, 319, 809
PfeI GAWTC 2 cut(s) 379, 847
PkrI GCNGC 4 cut(s) 247, 268, 454, 550
PleI GAGTC 2 cut(s) 102, 188
PpsI GAGTC 2 cut(s) 102, 188
PshAI GACNNNNGTC 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 349, 644
PspPI GGNCC 3 cut(s) 179, 536, 700
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 3 cut(s) 387, 389, 648
SaqAI TTAA 4 cut(s) 207, 402, 744, 816
SatI GCNGC 4 cut(s) 246, 267, 453, 549
Sau96I GGNCC 3 cut(s) 179, 536, 700
SchI GAGTC 2 cut(s) 102, 188
SduI GDGCHC 1 cut(s) 645
SfaNI GCATC 2 cut(s) 32, 180
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 3 cut(s) 387, 389, 648
SmlI CTYRAG 3 cut(s) 110, 139, 824
SmoI CTYRAG 3 cut(s) 110, 139, 824
Sse9I AATT 3 cut(s) 261, 342, 853
SseBI AGGCCT 3 cut(s) 73, 319, 809
SspMI CTAG 1 cut(s) 686
StuI AGGCCT 3 cut(s) 73, 319, 809
StyI CCWWGG 3 cut(s) 68, 182, 810
TaaI ACNGT 2 cut(s) 160, 293
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 106
TaqII GACCGA 1 cut(s) 524
TasI AATT 3 cut(s) 261, 342, 853
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 2 cut(s) 379, 847
Tru1I TTAA 4 cut(s) 207, 402, 744, 816
Tru9I TTAA 4 cut(s) 207, 402, 744, 816
TscAI CASTG 2 cut(s) 205, 523
TseFI GTSAC 3 cut(s) 238, 391, 758
TseI GCWGC 4 cut(s) 245, 266, 452, 548
Tsp45I GTSAC 3 cut(s) 238, 391, 758
TspDTI ATGAA 4 cut(s) 36, 75, 607, 763
TspRI CASTG 2 cut(s) 205, 523
VpaK11BI GGWCC 1 cut(s) 536
XspI CTAG 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.