MD13G1130900.v1.1

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013259
Physical Location & Seq
Forward (+)
9959122 .. 9961647
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1130900.v1.1.491

Sequence Viewer

Length: 900 bp
ATGGGTTCCAGAATGCTGTGTCTGTCTGCAGCCATAGTCCCTCCTCCTGCGCCTGCTCCTGCTGTAAAGCTTACCACATCAAATAATATGTCCAAGATTTTTCGTTCCTCTTCTGCAGATAAAGCTTCGGCTCGGCTTCAATCTTTCAGGTGCAGAGCTGTCGGAGAAAGCTCTCAGCCGCAGACCTCGTTGGCTGAAACCGTATATCAGGGAGTTTACGGTCCTTGGTCCGTCGACTCTACCGACGTCAGAGAGGTTATTTTGTACAGGTCGGGACTAGTGACTGCAGCTTCATCATTTGTAGTTGCAGCTTCTGCTGCATTTTTGCCTGATAGTTTCTTCTTAACTGACATTGTCAAACACAATCTCGATTTTGTCTACGCGATCGGAGCTGGTGGATTGGGGTTGTCCCTTTTTCTGATTCACATCTATGTGACTGAAATTAAGCGCACTCTGCAAGCATTTTGGGGGCTTGGTGTTGCCGGGTCTTTCGCAACATATGCATGCTTGGCTAGACCAGCTGGAGAGGGTTTGGTGCAATATGTTGTGGATAACCCGGTTGCTATCTGGTTTGTTGGTCCTCTCTTTGCTTCTCTCACAGGACTAGTCTTCAAGGAAGGTCTCTGCTATGGAAAGTTGGAAGCTGGAATTCTCACATTCATCATACCTACCGTTCTTCTCGGGCACTTGAGTGGACTGATGGACGATGGAGCAAAACTTTCTTTGCTCGGTTTGTGGATGGCCCTGTTTGTAATTTTTGCCGGAAGGAAGTTCACTCAACCGATCAAGGATGACATTGGCGACAAGTCCGTCTTTACATTCAATTCTCTACCTGAAGACGAGAAGATGGCCTTGATTCAGAAGCTTGAGCAACAAAAGTTAAGTCAGGATGTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

300

Amino Acids

32.16

Weight (kDa)

6.31

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 136 - 274 9.6e-54 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 809
AatII GACGTC 1 cut(s) 249
AccI GTMKAC 2 cut(s) 234, 378
AccII CGCG 1 cut(s) 383
AciI CCGC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 648
AcuI CTGAAG 1 cut(s) 855
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 1 cut(s) 266
AgsI TTSAA 3 cut(s) 140, 613, 823
AhlI ACTAGT 2 cut(s) 277, 604
AleI CACNNNNGTG 1 cut(s) 690
Alw26I GTCTC 1 cut(s) 626
AlwNI CAGNNNCTG 1 cut(s) 314
Ama87I CYCGRG 1 cut(s) 680
AoxI GGCC 2 cut(s) 741, 849
ApeKI GCWGC 4 cut(s) 29, 287, 308, 317
ApoI RAATTY 1 cut(s) 648
ArsI GACNNNNNNTTYG 4 cut(s) 74, 106, 868, 900
AspLEI GCGC 2 cut(s) 52, 450
AspS9I GGNCC 4 cut(s) 221, 228, 578, 742
AsuC2I CCSGG 2 cut(s) 484, 557
AvaI CYCGRG 1 cut(s) 680
AvaII GGWCC 3 cut(s) 221, 228, 578
BaeGI GKGCMC 1 cut(s) 687
BbsI GAAGAC 2 cut(s) 601, 843
BbvI GCAGC 4 cut(s) 41, 299, 304, 320
BccI CCATC 4 cut(s) 694, 701, 733, 841
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BcnI CCSGG 2 cut(s) 484, 557
BcoDI GTCTC 1 cut(s) 626
BcuI ACTAGT 2 cut(s) 277, 604
BfaI CTAG 3 cut(s) 278, 513, 605
BfmI CTRYAG 3 cut(s) 27, 114, 285
BisI GCNGC 5 cut(s) 30, 179, 288, 309, 318
BlsI GCNGC 5 cut(s) 31, 180, 289, 310, 319
Bme1390I CCNGG 2 cut(s) 484, 557
Bme18I GGWCC 3 cut(s) 221, 228, 578
BmeT110I CYCGRG 1 cut(s) 680
BmgT120I GGNCC 4 cut(s) 221, 228, 578, 742
BmiI GGNNCC 1 cut(s) 7
BmrFI CCNGG 2 cut(s) 484, 557
BpiI GAAGAC 2 cut(s) 601, 843
BpmI CTGGAG 1 cut(s) 543
BpuEI CTTGAG 2 cut(s) 709, 887
BpuMI CCSGG 2 cut(s) 484, 557
BsaBI GATNNNNATC 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 246
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 1 cut(s) 224
Bse8I GATNNNNATC 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 224
BseGI GGATG 3 cut(s) 744, 796, 895
BseJI GATNNNNATC 1 cut(s) 425
BseMII CTCAG 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 33
BseSI GKGCMC 1 cut(s) 687
BseXI GCAGC 4 cut(s) 41, 299, 304, 320
BsgI GTGCAG 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 383
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 2 cut(s) 743, 851
BsiEI CGRYCG 1 cut(s) 387
BsiHKCI CYCGRG 1 cut(s) 680
BsiSI CCGG 3 cut(s) 483, 557, 762
BslFI GGGAC 3 cut(s) 23, 288, 394
BsmAI GTCTC 1 cut(s) 626
BsmFI GGGAC 3 cut(s) 23, 288, 394
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 2 cut(s) 743, 851
Bso31I GGTCTC 1 cut(s) 626
BsoBI CYCGRG 1 cut(s) 680
Bsp1286I GDGCHC 1 cut(s) 687
Bsp1407I TGTACA 1 cut(s) 264
Bsp143I GATC 2 cut(s) 384, 783
BspACI CCGC 1 cut(s) 179
BspANI GGCC 2 cut(s) 743, 851
BspCNI CTCAG 1 cut(s) 187
BspFNI CGCG 1 cut(s) 383
BspLI GGNNCC 1 cut(s) 7
BspMAI CTGCAG 3 cut(s) 31, 118, 289
BspTNI GGTCTC 1 cut(s) 626
BsrGI TGTACA 1 cut(s) 264
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 2 cut(s) 384, 783
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 224
Bst4CI ACNGT 3 cut(s) 202, 221, 673
Bst6I CTCTTC 1 cut(s) 115
BstACI GRCGYC 1 cut(s) 246
BstAPI GCANNNNNTGC 2 cut(s) 314, 500
BstAUI TGTACA 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 54, 459, 505
BstDEI CTNAG 1 cut(s) 174
BstF5I GGATG 3 cut(s) 744, 796, 895
BstFNI CGCG 1 cut(s) 383
BstHHI GCGC 2 cut(s) 52, 450
BstKTI GATC 2 cut(s) 387, 786
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 2 cut(s) 384, 783
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 8 cut(s) 122, 314, 317, 389, 454, 500, 509, 518
BstNSI RCATGY 1 cut(s) 507
BstSCI CCNGG 2 cut(s) 482, 555
BstSFI CTRYAG 3 cut(s) 27, 114, 285
BstSLI GKGCMC 1 cut(s) 687
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 4 cut(s) 41, 299, 304, 320
BstV2I GAAGAC 2 cut(s) 601, 843
BsuRI GGCC 2 cut(s) 743, 851
BtsCI GGATG 3 cut(s) 744, 796, 895
Cac8I GCNNGC 3 cut(s) 54, 459, 505
CaiI CAGNNNCTG 1 cut(s) 314
CfoI GCGC 2 cut(s) 52, 450
Cfr13I GGNCC 4 cut(s) 221, 228, 578, 742
Csp6I GTAC 1 cut(s) 265
CviAII CATG 1 cut(s) 504
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 1 cut(s) 174
DpnI GATC 2 cut(s) 386, 785
DpnII GATC 2 cut(s) 384, 783
DrdI GACNNNNNNGTC 1 cut(s) 809
DseDI GACNNNNNNGTC 1 cut(s) 809
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 224
Eco31I GGTCTC 1 cut(s) 626
Eco47I GGWCC 3 cut(s) 221, 228, 578
Eco57I CTGAAG 1 cut(s) 855
Eco88I CYCGRG 1 cut(s) 680
EcoRI GAATTC 1 cut(s) 648
EcoT14I CCWWGG 1 cut(s) 224
EcoT22I ATGCAT 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 1 cut(s) 507
FalI AAGNNNNNCTT 4 cut(s) 797, 829, 836, 868
FaqI GGGAC 3 cut(s) 23, 288, 394
FatI CATG 1 cut(s) 503
FauNDI CATATG 1 cut(s) 499
FblI GTMKAC 2 cut(s) 234, 378
Fnu4HI GCNGC 5 cut(s) 30, 179, 288, 309, 318
FokI GGATG 2 cut(s) 751, 803
Fsp4HI GCNGC 5 cut(s) 30, 179, 288, 309, 318
FspBI CTAG 3 cut(s) 278, 513, 605
GlaI GCGC 2 cut(s) 51, 449
GluI GCNGC 5 cut(s) 30, 179, 288, 309, 318
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 2 cut(s) 743, 851
HapII CCGG 3 cut(s) 483, 557, 762
HhaI GCGC 2 cut(s) 52, 450
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 1 cut(s) 507
Hin6I GCGC 2 cut(s) 50, 448
HinP1I GCGC 2 cut(s) 50, 448
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 3 cut(s) 68, 123, 863
HinfI GANTC 3 cut(s) 236, 421, 856
HpaII CCGG 3 cut(s) 483, 557, 762
Hpy166II GTNNAC 5 cut(s) 217, 235, 379, 695, 774
Hpy188I TCNGA 5 cut(s) 164, 251, 389, 420, 861
Hpy188III TCNNGA 4 cut(s) 9, 273, 368, 887
Hpy8I GTNNAC 5 cut(s) 217, 235, 379, 695, 774
Hpy99I CGWCG 2 cut(s) 236, 248
HpyAV CCTTC 2 cut(s) 611, 759
HpyCH4III ACNGT 3 cut(s) 202, 221, 673
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 9 cut(s) 29, 116, 153, 287, 308, 320, 457, 503, 538
HpyF10VI GCNNNNNNNGC 8 cut(s) 122, 314, 317, 389, 454, 500, 509, 518
HpyF3I CTNAG 1 cut(s) 174
HpySE526I ACGT 1 cut(s) 246
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 1 cut(s) 507
HspAI GCGC 2 cut(s) 50, 448
Kzo9I GATC 2 cut(s) 384, 783
LmnI GCTCC 3 cut(s) 61, 389, 710
Lsp1109I GCAGC 4 cut(s) 41, 299, 304, 320
MaeI CTAG 3 cut(s) 278, 513, 605
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 2 cut(s) 280, 433
MalI GATC 2 cut(s) 386, 785
MboI GATC 2 cut(s) 384, 783
MboII GAAGA 6 cut(s) 102, 331, 601, 668, 848, 856
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 4 cut(s) 441, 648, 753, 823
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 142, 618
MnlI CCTC 7 cut(s) 51, 54, 118, 196, 247, 520, 591
Mph1103I ATGCAT 1 cut(s) 505
MseI TTAA 3 cut(s) 344, 444, 881
MslI CAYNNNNRTG 4 cut(s) 429, 431, 502, 690
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 3 cut(s) 483, 557, 762
MspR9I CCNGG 2 cut(s) 484, 557
Mva1269I GAATGC 1 cut(s) 18
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 8 cut(s) 122, 314, 317, 389, 454, 500, 509, 518
NciI CCSGG 2 cut(s) 484, 557
NdeI CATATG 1 cut(s) 499
NdeII GATC 2 cut(s) 384, 783
NlaIII CATG 1 cut(s) 507
NlaIV GGNNCC 1 cut(s) 7
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 280, 433
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 1 cut(s) 507
OliI CACNNNNGTG 1 cut(s) 690
PaeI GCATGC 1 cut(s) 507
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 421, 856
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 5 cut(s) 31, 180, 289, 310, 319
Ple19I CGATCG 1 cut(s) 387
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PspN4I GGNNCC 1 cut(s) 7
PspPI GGNCC 4 cut(s) 221, 228, 578, 742
PstI CTGCAG 3 cut(s) 31, 118, 289
PstNI CAGNNNCTG 1 cut(s) 314
PsyI GACNNNGTC 1 cut(s) 353
PvuI CGATCG 1 cut(s) 387
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 4 cut(s) 429, 431, 502, 690
SalI GTCGAC 1 cut(s) 233
SaqAI TTAA 3 cut(s) 344, 444, 881
SatI GCNGC 5 cut(s) 30, 179, 288, 309, 318
Sau3AI GATC 2 cut(s) 384, 783
Sau96I GGNCC 4 cut(s) 221, 228, 578, 742
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 484, 557
SduI GDGCHC 1 cut(s) 687
SfcI CTRYAG 3 cut(s) 27, 114, 285
SinI GGWCC 3 cut(s) 221, 228, 578
SmiMI CAYNNNNRTG 4 cut(s) 429, 431, 502, 690
SmlI CTYRAG 2 cut(s) 688, 866
SmoI CTYRAG 2 cut(s) 688, 866
SpeI ACTAGT 2 cut(s) 277, 604
SphI GCATGC 1 cut(s) 507
Sse9I AATT 4 cut(s) 441, 648, 753, 823
SsiI CCGC 1 cut(s) 179
SspMI CTAG 3 cut(s) 278, 513, 605
StyD4I CCNGG 2 cut(s) 482, 555
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 202, 221, 673
TaiI ACGT 1 cut(s) 249
TaqI TCGA 3 cut(s) 234, 369, 894
TasI AATT 4 cut(s) 441, 648, 753, 823
TatI WGTACW 1 cut(s) 264
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 2 cut(s) 421, 856
Tru1I TTAA 3 cut(s) 344, 444, 881
Tru9I TTAA 3 cut(s) 344, 444, 881
TseFI GTSAC 2 cut(s) 280, 433
TseI GCWGC 4 cut(s) 29, 287, 308, 317
Tsp45I GTSAC 2 cut(s) 280, 433
TspDTI ATGAA 2 cut(s) 282, 649
TspGWI ACGGA 2 cut(s) 220, 799
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 3 cut(s) 221, 228, 578
XapI RAATTY 1 cut(s) 648
XceI RCATGY 1 cut(s) 507
XmiI GTMKAC 2 cut(s) 234, 378
XspI CTAG 3 cut(s) 278, 513, 605
ZraI GACGTC 1 cut(s) 247
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.