Rorug04G0229900

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013259
Physical Location & Seq
Forward (+)
39424556 .. 39431172
6617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0229900.1

Sequence Viewer

Length: 582 bp
ATGAGCCGAGACTGCTTCGAGGAGGAAAACAAGGATGTGGTATCAGCTCCTAGACCGAGTTGCCCTCCTATTAGGAGAATAACAGCTACAAGAGATAAGGAGGATAATGCATTGGAATATGGATTGCCATCTTCTCACACTATATACACAGTTTGCTTGTCTAGATACCTTTTGCATTGCATCCTATCTTATGCTCAGAATGAAGAGGCCTATGTGAAATTTGCTAGATTTGCTCTTGTTTGCTTGCTCGTTGGTCTCATTGGTTTGGTTATGCACAATACTGACAGTTTTGATAAATGGAAAGGATCGATGCTGAAGAAAGGAGTTGAAGAAGGAAGATGGACGGAGAAATTTGTTTCCCGTGTTCAATTCAGTGGAGACCTTGTTACTGCATCTCCAGACGTTTATCAAGCAAGTCTAGGGGCAGACTCAGAATTTCTAGTGTTAGCATCTGATGGTTTATGGGATTACATAAACAGTTCAGATGCAGTTTCTTTTGTAAGGAATCAGCTTCGACAACATGGAGATGTCCAGCTAGCTTGTTATGCGCTTGCTGAAGCTGCTCTGGCAACAACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

193

Amino Acids

21.53

Weight (kDa)

5.22

Isoelectric Point (pI)

53.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 127 - 191 3.1e-15 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 313
AcsI RAATTY 3 cut(s) 218, 350, 434
AcuI CTGAAG 2 cut(s) 335, 576
AgsI TTSAA 2 cut(s) 329, 368
AluBI AGCT 6 cut(s) 47, 86, 511, 535, 539, 560
AluI AGCT 6 cut(s) 47, 86, 511, 535, 539, 560
Alw26I GTCTC 3 cut(s) 3, 260, 372
AlwI GGATC 1 cut(s) 313
AoxI GGCC 1 cut(s) 207
ApeKI GCWGC 1 cut(s) 560
ApoI RAATTY 3 cut(s) 218, 350, 434
AspLEI GCGC 1 cut(s) 550
AsuNHI GCTAGC 1 cut(s) 535
BbvI GCAGC 1 cut(s) 547
BccI CCATC 3 cut(s) 136, 333, 449
BcoDI GTCTC 3 cut(s) 3, 260, 372
BfaI CTAG 6 cut(s) 51, 162, 225, 419, 440, 536
BisI GCNGC 1 cut(s) 561
BlsI GCNGC 1 cut(s) 562
BmsI GCATC 5 cut(s) 189, 300, 401, 458, 475
BmtI GCTAGC 1 cut(s) 539
BplI GAGNNNNNCTC 2 cut(s) 49, 81
BpmI CTGGAG 1 cut(s) 381
Bsa29I ATCGAT 1 cut(s) 308
BsaBI GATNNNNATC 1 cut(s) 127
BsaI GGTCTC 2 cut(s) 260, 372
BsaXI ACNNNNNCTCC 4 cut(s) 369, 379, 399, 409
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 127
BseCI ATCGAT 1 cut(s) 308
BseGI GGATG 2 cut(s) 40, 180
BseJI GATNNNNATC 1 cut(s) 127
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 209, 444
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 547
BshFI GGCC 1 cut(s) 209
BshVI ATCGAT 1 cut(s) 308
BsmAI GTCTC 3 cut(s) 3, 260, 372
BsnI GGCC 1 cut(s) 209
Bso31I GGTCTC 2 cut(s) 260, 372
Bsp143I GATC 1 cut(s) 305
BspANI GGCC 1 cut(s) 209
BspCNI CTCAG 2 cut(s) 208, 443
BspDI ATCGAT 1 cut(s) 308
BspOI GCTAGC 1 cut(s) 539
BspPI GGATC 1 cut(s) 313
BspTNI GGTCTC 2 cut(s) 260, 372
BsrDI GCAATG 1 cut(s) 175
BssMI GATC 1 cut(s) 305
Bst4CI ACNGT 3 cut(s) 151, 287, 479
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 3 cut(s) 245, 537, 552
BstDEI CTNAG 2 cut(s) 195, 430
BstF5I GGATG 2 cut(s) 40, 180
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 1 cut(s) 308
BstMAI GTCTC 3 cut(s) 3, 260, 372
BstMBI GATC 1 cut(s) 305
BstMWI GCNNNNNNNGC 5 cut(s) 12, 230, 545, 560, 566
BstV1I GCAGC 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 308
BsuRI GGCC 1 cut(s) 209
BsuTUI ATCGAT 1 cut(s) 308
BtsCI GGATG 2 cut(s) 40, 180
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 3 cut(s) 245, 537, 552
CfoI GCGC 1 cut(s) 550
ClaI ATCGAT 1 cut(s) 308
CviAII CATG 1 cut(s) 521
CviJI RGCY 8 cut(s) 6, 47, 86, 209, 511, 535, 539, 560
CviKI_1 RGCY 8 cut(s) 6, 47, 86, 209, 511, 535, 539, 560
DdeI CTNAG 2 cut(s) 195, 430
DpnI GATC 1 cut(s) 307
DpnII GATC 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco147I AGGCCT 1 cut(s) 209
Eco31I GGTCTC 2 cut(s) 260, 372
Eco57I CTGAAG 2 cut(s) 335, 576
EcoT22I ATGCAT 1 cut(s) 112
FaeI CATG 1 cut(s) 524
FatI CATG 1 cut(s) 520
Fnu4HI GCNGC 1 cut(s) 561
FokI GGATG 2 cut(s) 47, 167
Fsp4HI GCNGC 1 cut(s) 561
FspBI CTAG 6 cut(s) 51, 162, 225, 419, 440, 536
GlaI GCGC 1 cut(s) 549
GluI GCNGC 1 cut(s) 561
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 209
HhaI GCGC 1 cut(s) 550
Hin1II CATG 1 cut(s) 524
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HinfI GANTC 2 cut(s) 428, 505
Hpy188I TCNGA 4 cut(s) 198, 433, 454, 484
Hpy188III TCNNGA 2 cut(s) 162, 398
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 3 cut(s) 151, 287, 479
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 6 cut(s) 110, 175, 180, 274, 392, 488
HpyF10VI GCNNNNNNNGC 5 cut(s) 12, 230, 545, 560, 566
HpyF3I CTNAG 2 cut(s) 195, 430
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 1 cut(s) 524
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 1 cut(s) 305
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 3 cut(s) 411, 545, 551
Lsp1109I GCAGC 1 cut(s) 547
LweI GCATC 5 cut(s) 189, 300, 401, 458, 475
MaeI CTAG 6 cut(s) 51, 162, 225, 419, 440, 536
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 385
MalI GATC 1 cut(s) 307
MboI GATC 1 cut(s) 305
MboII GAAGA 5 cut(s) 123, 215, 328, 341, 348
MluCI AATT 5 cut(s) 218, 350, 368, 434, 577
MlyI GAGTC 1 cut(s) 422
MnlI CCTC 5 cut(s) 13, 16, 75, 94, 199
Mph1103I ATGCAT 1 cut(s) 112
MslI CAYNNNNRTG 1 cut(s) 525
MwoI GCNNNNNNNGC 5 cut(s) 12, 230, 545, 560, 566
NdeII GATC 1 cut(s) 305
NheI GCTAGC 1 cut(s) 535
NlaIII CATG 1 cut(s) 524
NmeAIII GCCGAG 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 112
PceI AGGCCT 1 cut(s) 209
PfeI GAWTC 1 cut(s) 505
PkrI GCNGC 1 cut(s) 562
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
RseI CAYNNNNRTG 1 cut(s) 525
SatI GCNGC 1 cut(s) 561
Sau3AI GATC 1 cut(s) 305
SchI GAGTC 1 cut(s) 422
SetI ASST 9 cut(s) 49, 88, 171, 384, 405, 513, 537, 541, 562
SfaNI GCATC 5 cut(s) 189, 300, 401, 458, 475
SmiMI CAYNNNNRTG 1 cut(s) 525
Sse9I AATT 5 cut(s) 218, 350, 368, 434, 577
SseBI AGGCCT 1 cut(s) 209
SspMI CTAG 6 cut(s) 51, 162, 225, 419, 440, 536
StuI AGGCCT 1 cut(s) 209
TaaI ACNGT 3 cut(s) 151, 287, 479
TaiI ACGT 1 cut(s) 405
TaqI TCGA 3 cut(s) 18, 308, 514
TaqII GACCGA 1 cut(s) 70
TasI AATT 5 cut(s) 218, 350, 368, 434, 577
TfiI GAWTC 1 cut(s) 505
TscAI CASTG 1 cut(s) 379
TseI GCWGC 1 cut(s) 560
TspDTI ATGAA 1 cut(s) 216
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 1 cut(s) 379
XapI RAATTY 3 cut(s) 218, 350, 434
XbaI TCTAGA 1 cut(s) 161
XspI CTAG 6 cut(s) 51, 162, 225, 419, 440, 536
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.