RchiOBHm_Chr4g0428871

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013259
Physical Location & Seq
Reverse (-)
53789922 .. 53791654
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39772

Sequence Viewer

Length: 837 bp
ATGGCTTCACATATGTTGTGTGCATCCATTGTCACTCCTACTCCTGCATCAAACCTCACCAAGCTTCCAAGGCCTTCTTCTTCTTCTTCACCCAGACTGCCAATTCGACTCGGGTCTTTCAAGTGCAAGGCTCAAAGCTCAAGCTCTCAGTCTCAGACTGTTTATCAGGGCGTCTATGGCCCTTGGTCTGTTGACTCCACCGACGTTAAAGAGGTGATTCTGTACAGGTCTGGATTGGTGACTGCAGCTACGTCATTTGTAATTGCAGCTTCTACTGCTTTTTTGCCCGACAGTTTCTTGGCCACTGAGATTGTTAAGCCCAATCTTGATTACATATACGCAATTGGAGCCGGTGGGTTGGGTTTGTCGTTGGTTCTGATTCACATTTATGTGACTGAGATTAAGCGCACTTTGCAAGCGTTTTGGGGGCTTGGTGTTGTTGGGTCTTTGGCTACATATGTATGCTTGGCTCAACCAGCTGGAGAGGGCTTGGTTCAGTATGTTGTTGATAGCCCAGTTGCTGTTTGGTTTGTTGGTCCTTTGTTTGCAGCGCTCACAGGACTGGTTTTCAAGGAAGGTCTTTGCTATGGAAAGTTGGAAGCTGGAATTCTCACATTCATTATACCCATTGTTCTTCTTGGGCACCTGAGTGGGTTGATGGATGATGGAGTGAAACTTTCTCTACTGGGTTCATGGATGGCCCTGTTTGTAATATTTGCTGGCAGGAAGTTCACACAACCCATTAAGGATGACATCGGTGACAAGTCTGTCTTCATATTCCAGTCTCTGCCCGAGGACGAGAAGAAGGCCTTGGTTAAGAAGCTTGAGCAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

278

Amino Acids

29.8

Weight (kDa)

8.26

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 122 - 260 2.1e-54 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 767
AccB1I GGYRCC 1 cut(s) 642
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 606
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 1 cut(s) 224
AfeI AGCGCT 1 cut(s) 552
AgsI TTSAA 2 cut(s) 121, 571
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 794, 826
AleI CACNNNNGTG 1 cut(s) 648
AluBI AGCT 8 cut(s) 64, 138, 144, 248, 269, 479, 602, 823
AluI AGCT 8 cut(s) 64, 138, 144, 248, 269, 479, 602, 823
Alw26I GTCTC 2 cut(s) 156, 789
AlwNI CAGNNNCTG 2 cut(s) 521, 787
Ama87I CYCGRG 2 cut(s) 110, 791
Aor51HI AGCGCT 1 cut(s) 552
AoxI GGCC 5 cut(s) 71, 178, 300, 699, 807
ApeKI GCWGC 3 cut(s) 245, 266, 548
ApoI RAATTY 1 cut(s) 606
ArsI GACNNNNNNTTYG 2 cut(s) 87, 119
AspLEI GCGC 2 cut(s) 408, 553
AspS9I GGNCC 3 cut(s) 179, 536, 700
AsuHPI GGTGA 5 cut(s) 49, 81, 226, 250, 770
AvaI CYCGRG 2 cut(s) 110, 791
AvaII GGWCC 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 645
BalI TGGCCA 1 cut(s) 302
BanI GGYRCC 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 763
BbvI GCAGC 3 cut(s) 257, 278, 560
BccI CCATC 3 cut(s) 652, 659, 691
BcoDI GTCTC 2 cut(s) 156, 789
BfmI CTRYAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 554
BisI GCNGC 3 cut(s) 246, 267, 549
BlsI GCNGC 3 cut(s) 247, 268, 550
Bme18I GGWCC 1 cut(s) 536
BmeT110I CYCGRG 2 cut(s) 110, 791
BmgT120I GGNCC 3 cut(s) 179, 536, 700
BmiI GGNNCC 2 cut(s) 349, 644
BmrI ACTGGG 2 cut(s) 509, 695
BmsI GCATC 2 cut(s) 32, 56
BmuI ACTGGG 2 cut(s) 509, 695
BoxI GACNNNNGTC 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 763
BpmI CTGGAG 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 124
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 4 cut(s) 68, 182, 792, 810
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 4 cut(s) 515, 567, 690, 781
BseDI CCNNGG 4 cut(s) 68, 182, 792, 810
BseGI GGATG 4 cut(s) 23, 667, 702, 754
BseMII CTCAG 5 cut(s) 161, 167, 297, 387, 638
BseNI ACTGG 4 cut(s) 515, 567, 690, 781
BseSI GKGCMC 1 cut(s) 645
BseXI GCAGC 3 cut(s) 257, 278, 560
BshFI GGCC 5 cut(s) 73, 180, 302, 701, 809
BshNI GGYRCC 1 cut(s) 642
BsiHKCI CYCGRG 2 cut(s) 110, 791
BsiSI CCGG 1 cut(s) 351
BsmAI GTCTC 2 cut(s) 156, 789
BsnI GGCC 5 cut(s) 73, 180, 302, 701, 809
BsoBI CYCGRG 2 cut(s) 110, 791
Bsp1286I GDGCHC 1 cut(s) 645
Bsp1407I TGTACA 1 cut(s) 222
BspANI GGCC 5 cut(s) 73, 180, 302, 701, 809
BspCNI CTCAG 5 cut(s) 160, 166, 298, 388, 639
BspLI GGNNCC 2 cut(s) 349, 644
BspMAI CTGCAG 1 cut(s) 247
BspT107I GGYRCC 1 cut(s) 642
BsrFI RCCGGY 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 4 cut(s) 515, 567, 690, 781
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 4 cut(s) 68, 182, 792, 810
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 3 cut(s) 68, 182, 810
Bst4CI ACNGT 3 cut(s) 160, 293, 834
BstACI GRCGYC 1 cut(s) 171
BstAUI TGTACA 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 417, 721
BstDEI CTNAG 5 cut(s) 147, 153, 306, 396, 647
BstF5I GGATG 4 cut(s) 23, 667, 702, 754
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 2 cut(s) 408, 553
BstMAI GTCTC 2 cut(s) 156, 789
BstMWI GCNNNNNNNGC 6 cut(s) 70, 177, 275, 347, 412, 476
BstPAI GACNNNNGTC 1 cut(s) 112
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 645
BstV1I GCAGC 3 cut(s) 257, 278, 560
BstV2I GAAGAC 1 cut(s) 763
BsuRI GGCC 5 cut(s) 73, 180, 302, 701, 809
BtsCI GGATG 4 cut(s) 23, 667, 702, 754
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 417, 721
CaiI CAGNNNCTG 2 cut(s) 521, 787
CfoI GCGC 2 cut(s) 408, 553
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 3 cut(s) 179, 536, 700
CseI GACGC 1 cut(s) 160
Csp6I GTAC 1 cut(s) 223
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 1 cut(s) 693
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 5 cut(s) 147, 153, 306, 396, 647
DrdI GACNNNNNNGTC 1 cut(s) 767
DseDI GACNNNNNNGTC 1 cut(s) 767
EaeI YGGCCR 1 cut(s) 300
Eco130I CCWWGG 3 cut(s) 68, 182, 810
Eco147I AGGCCT 2 cut(s) 73, 809
Eco47I GGWCC 1 cut(s) 536
Eco47III AGCGCT 1 cut(s) 552
Eco88I CYCGRG 2 cut(s) 110, 791
EcoRI GAATTC 1 cut(s) 606
EcoT14I CCWWGG 3 cut(s) 68, 182, 810
ErhI CCWWGG 3 cut(s) 68, 182, 810
FaeI CATG 1 cut(s) 696
FalI AAGNNNNNCTT 6 cut(s) 61, 93, 755, 787, 794, 826
FatI CATG 1 cut(s) 692
FauNDI CATATG 2 cut(s) 12, 457
Fnu4HI GCNGC 3 cut(s) 246, 267, 549
FokI GGATG 4 cut(s) 10, 674, 709, 761
Fsp4HI GCNGC 3 cut(s) 246, 267, 549
GlaI GCGC 2 cut(s) 407, 552
GluI GCNGC 3 cut(s) 246, 267, 549
GsuI CTGGAG 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 5 cut(s) 73, 180, 302, 701, 809
HapII CCGG 1 cut(s) 351
HgaI GACGC 1 cut(s) 160
HhaI GCGC 2 cut(s) 408, 553
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 1 cut(s) 696
Hin6I GCGC 2 cut(s) 406, 551
HinP1I GCGC 2 cut(s) 406, 551
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 2 cut(s) 62, 821
HinfI GANTC 4 cut(s) 108, 194, 217, 379
HpaII CCGG 1 cut(s) 351
HphI GGTGA 5 cut(s) 49, 81, 226, 250, 770
Hpy166II GTNNAC 2 cut(s) 193, 732
Hpy188I TCNGA 2 cut(s) 156, 378
Hpy188III TCNNGA 2 cut(s) 231, 326
Hpy8I GTNNAC 2 cut(s) 193, 732
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 3 cut(s) 84, 569, 799
HpyCH4III ACNGT 3 cut(s) 160, 293, 834
HpyCH4IV ACGT 2 cut(s) 204, 251
HpyCH4V TGCA 7 cut(s) 23, 47, 126, 245, 266, 415, 548
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 177, 275, 347, 412, 476
HpyF3I CTNAG 5 cut(s) 147, 153, 306, 396, 647
HpySE526I ACGT 2 cut(s) 204, 251
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 1 cut(s) 696
HspAI GCGC 2 cut(s) 406, 551
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 3 cut(s) 257, 278, 560
LweI GCATC 2 cut(s) 32, 56
MaeII ACGT 2 cut(s) 204, 251
MaeIII GTNAC 4 cut(s) 31, 238, 391, 758
MboII GAAGA 7 cut(s) 69, 72, 75, 78, 626, 763, 814
MfeI CAATTG 1 cut(s) 342
MhlI GDGCHC 1 cut(s) 645
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 4 cut(s) 102, 261, 342, 606
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 2 cut(s) 102, 188
MmeI TCCRAC 1 cut(s) 576
MnlI CCTC 4 cut(s) 65, 205, 478, 787
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 5 cut(s) 207, 315, 402, 744, 816
MslI CAYNNNNRTG 4 cut(s) 387, 389, 460, 648
Msp20I TGGCCA 1 cut(s) 302
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 1 cut(s) 351
MunI CAATTG 1 cut(s) 342
MwoI GCNNNNNNNGC 6 cut(s) 70, 177, 275, 347, 412, 476
NdeI CATATG 2 cut(s) 12, 457
NlaIII CATG 1 cut(s) 696
NlaIV GGNNCC 2 cut(s) 349, 644
NmuCI GTSAC 4 cut(s) 31, 238, 391, 758
OliI CACNNNNGTG 1 cut(s) 648
PceI AGGCCT 2 cut(s) 73, 809
PfeI GAWTC 2 cut(s) 217, 379
PkrI GCNGC 3 cut(s) 247, 268, 550
PleI GAGTC 2 cut(s) 102, 188
PpsI GAGTC 2 cut(s) 102, 188
PshAI GACNNNNGTC 1 cut(s) 112
PspN4I GGNNCC 2 cut(s) 349, 644
PspPI GGNCC 3 cut(s) 179, 536, 700
PstI CTGCAG 1 cut(s) 247
PstNI CAGNNNCTG 2 cut(s) 521, 787
PvuII CAGCTG 1 cut(s) 479
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 4 cut(s) 387, 389, 460, 648
SaqAI TTAA 5 cut(s) 207, 315, 402, 744, 816
SatI GCNGC 3 cut(s) 246, 267, 549
Sau96I GGNCC 3 cut(s) 179, 536, 700
SchI GAGTC 2 cut(s) 102, 188
SduI GDGCHC 1 cut(s) 645
SfaNI GCATC 2 cut(s) 32, 56
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 4 cut(s) 387, 389, 460, 648
SmlI CTYRAG 2 cut(s) 139, 824
SmoI CTYRAG 2 cut(s) 139, 824
Sse9I AATT 4 cut(s) 102, 261, 342, 606
SseBI AGGCCT 2 cut(s) 73, 809
SspI AATATT 1 cut(s) 714
StuI AGGCCT 2 cut(s) 73, 809
StyI CCWWGG 3 cut(s) 68, 182, 810
TaaI ACNGT 3 cut(s) 160, 293, 834
TaiI ACGT 2 cut(s) 207, 254
TaqI TCGA 1 cut(s) 106
TasI AATT 4 cut(s) 102, 261, 342, 606
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 2 cut(s) 217, 379
Tru1I TTAA 5 cut(s) 207, 315, 402, 744, 816
Tru9I TTAA 5 cut(s) 207, 315, 402, 744, 816
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 4 cut(s) 31, 238, 391, 758
TseI GCWGC 3 cut(s) 245, 266, 548
Tsp45I GTSAC 4 cut(s) 31, 238, 391, 758
TspDTI ATGAA 3 cut(s) 607, 681, 763
TspRI CASTG 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 536
XapI RAATTY 1 cut(s) 606
XcmI CCANNNNNNNNNTGG 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.