Rh4AG286600

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013259
Physical Location & Seq
Reverse (-)
60068101 .. 60070064
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG286600.1

Sequence Viewer

Length: 765 bp
ATGGCTTCACATATGTTGTGTGCATCCATCGTCACTCCTACTCCTGCATCAAACCTCACCAAGCTTCCAAGGCCTTCTTCTTCTTCTTCATCACCACCCAGACTTCCAATTCGACTCGGGTCTTTCAAGTGCAAGGCTCAAAGCTCAAGCTCTCAGTCTCAGACTGTTTATCAGGGCGTCTATGGCCCTTGGTCTGTTGACTCCACCGACGTTAAAGAGGTGATTCTGTACAGGTCAGGATTGGTGACTGCAGCTACGTCATTTGTAATTGCAGCTTCTACTGCTTTTTTGCCCGACAGTTTCTTGGCCACTGAGATTGTTAAGCCCAATCTTGATTTCATATACGCAATTGGAGCTGGTGGGTTGGGTTTGTCGTTGGTTCTGATTCACATTTATGTGACTGAGATTAAGCGCACTTTGCAAGCGTTTTGGGGGCTTGGTGTTGTTGGGTCTTTGGCTACATATGTATGCTTGGCTCAACCAGCTGGAGAGGGCTTGGTTCAGTATGTTGTTGATAACCCAGTTGCTGTTTGGTTTGTTGGTCCTTTGTTTGCAGCGCTCACAGGACTGGTTTTCAAGGAAGGTCTTTGCTATGGAAAGTTGGAAGCTGGAATTCTCACATTTATTATACCCATTGTTCTTCTTGGGCACCTGAGTGGCTTGATGGATGATGGAGTGAAACTTTCTCTACTGGGTTCATGGATGGCCCTGTTTGTATTATTTGCTGGAAGGAAGTTCACACAACCCATTAAGGTAGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

254

Amino Acids

27.01

Weight (kDa)

8.87

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 124 - 251 5.7e-46 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 648
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 230
AfeI AGCGCT 1 cut(s) 558
AgsI TTSAA 2 cut(s) 127, 577
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 320, 352
AleI CACNNNNGTG 1 cut(s) 654
AluBI AGCT 8 cut(s) 64, 144, 150, 254, 275, 356, 485, 608
AluI AGCT 8 cut(s) 64, 144, 150, 254, 275, 356, 485, 608
Alw26I GTCTC 1 cut(s) 162
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 116
Aor51HI AGCGCT 1 cut(s) 558
AoxI GGCC 4 cut(s) 71, 184, 306, 705
ApeKI GCWGC 3 cut(s) 251, 272, 554
ApoI RAATTY 1 cut(s) 612
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
AspLEI GCGC 2 cut(s) 414, 559
AspS9I GGNCC 3 cut(s) 185, 542, 706
AsuHPI GGTGA 4 cut(s) 49, 84, 232, 256
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 651
BalI TGGCCA 1 cut(s) 308
BanI GGYRCC 1 cut(s) 648
BbsI GAAGAC 1 cut(s) 751
BbvI GCAGC 3 cut(s) 263, 284, 566
BccI CCATC 4 cut(s) 35, 658, 665, 697
BcoDI GTCTC 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 560
BisI GCNGC 3 cut(s) 252, 273, 555
BlsI GCNGC 3 cut(s) 253, 274, 556
Bme18I GGWCC 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 3 cut(s) 185, 542, 706
BmiI GGNNCC 1 cut(s) 650
BmrI ACTGGG 2 cut(s) 515, 701
BmsI GCATC 2 cut(s) 32, 56
BmuI ACTGGG 2 cut(s) 515, 701
BoxI GACNNNNGTC 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 751
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 68, 188
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bse1I ACTGG 3 cut(s) 521, 573, 696
BseDI CCNNGG 2 cut(s) 68, 188
BseGI GGATG 3 cut(s) 23, 673, 708
BseMII CTCAG 5 cut(s) 167, 173, 303, 393, 644
BseNI ACTGG 3 cut(s) 521, 573, 696
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 3 cut(s) 263, 284, 566
BshFI GGCC 4 cut(s) 73, 186, 308, 707
BshNI GGYRCC 1 cut(s) 648
BsiHKCI CYCGRG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 162
BsnI GGCC 4 cut(s) 73, 186, 308, 707
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 651
Bsp1407I TGTACA 1 cut(s) 228
BspANI GGCC 4 cut(s) 73, 186, 308, 707
BspCNI CTCAG 5 cut(s) 166, 172, 304, 394, 645
BspLI GGNNCC 1 cut(s) 650
BspMAI CTGCAG 1 cut(s) 253
BspT107I GGYRCC 1 cut(s) 648
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 3 cut(s) 521, 573, 696
BssECI CCNNGG 2 cut(s) 68, 188
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 2 cut(s) 68, 188
Bst4CI ACNGT 2 cut(s) 166, 299
BstACI GRCGYC 1 cut(s) 177
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 5 cut(s) 153, 159, 312, 402, 653
BstF5I GGATG 3 cut(s) 23, 673, 708
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 2 cut(s) 414, 559
BstMAI GTCTC 1 cut(s) 162
BstMWI GCNNNNNNNGC 6 cut(s) 70, 183, 281, 353, 418, 482
BstPAI GACNNNNGTC 1 cut(s) 118
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 1 cut(s) 651
BstV1I GCAGC 3 cut(s) 263, 284, 566
BstV2I GAAGAC 1 cut(s) 751
BsuRI GGCC 4 cut(s) 73, 186, 308, 707
BtsCI GGATG 3 cut(s) 23, 673, 708
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 1 cut(s) 423
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 2 cut(s) 414, 559
Cfr13I GGNCC 3 cut(s) 185, 542, 706
CseI GACGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 229
CspCI CAANNNNNGTGG 2 cut(s) 298, 333
CviAII CATG 1 cut(s) 699
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 5 cut(s) 153, 159, 312, 402, 653
EaeI YGGCCR 1 cut(s) 306
Eco130I CCWWGG 2 cut(s) 68, 188
Eco147I AGGCCT 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 542
Eco47III AGCGCT 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 116
EcoRI GAATTC 1 cut(s) 612
EcoT14I CCWWGG 2 cut(s) 68, 188
ErhI CCWWGG 2 cut(s) 68, 188
FaeI CATG 1 cut(s) 702
FalI AAGNNNNNCTT 3 cut(s) 61, 93, 743
FatI CATG 1 cut(s) 698
FauNDI CATATG 2 cut(s) 12, 463
Fnu4HI GCNGC 3 cut(s) 252, 273, 555
FokI GGATG 3 cut(s) 10, 680, 715
Fsp4HI GCNGC 3 cut(s) 252, 273, 555
GlaI GCGC 2 cut(s) 413, 558
GluI GCNGC 3 cut(s) 252, 273, 555
GsuI CTGGAG 1 cut(s) 507
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 4 cut(s) 73, 186, 308, 707
HgaI GACGC 1 cut(s) 166
HhaI GCGC 2 cut(s) 414, 559
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 1 cut(s) 702
Hin6I GCGC 2 cut(s) 412, 557
HinP1I GCGC 2 cut(s) 412, 557
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 4 cut(s) 114, 200, 223, 385
HphI GGTGA 4 cut(s) 49, 84, 232, 256
Hpy166II GTNNAC 2 cut(s) 199, 738
Hpy188I TCNGA 2 cut(s) 162, 384
Hpy188III TCNNGA 2 cut(s) 237, 332
Hpy8I GTNNAC 2 cut(s) 199, 738
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 3 cut(s) 84, 575, 723
HpyCH4III ACNGT 2 cut(s) 166, 299
HpyCH4IV ACGT 2 cut(s) 210, 257
HpyCH4V TGCA 7 cut(s) 23, 47, 132, 251, 272, 421, 554
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 183, 281, 353, 418, 482
HpyF3I CTNAG 5 cut(s) 153, 159, 312, 402, 653
HpySE526I ACGT 2 cut(s) 210, 257
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 1 cut(s) 702
HspAI GCGC 2 cut(s) 412, 557
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 3 cut(s) 263, 284, 566
LweI GCATC 2 cut(s) 32, 56
MaeII ACGT 2 cut(s) 210, 257
MaeIII GTNAC 3 cut(s) 31, 244, 397
MboII GAAGA 6 cut(s) 69, 72, 75, 78, 632, 751
MfeI CAATTG 1 cut(s) 348
MhlI GDGCHC 1 cut(s) 651
MlsI TGGCCA 1 cut(s) 308
MluCI AATT 4 cut(s) 108, 267, 348, 612
MluNI TGGCCA 1 cut(s) 308
MlyI GAGTC 2 cut(s) 108, 194
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 3 cut(s) 65, 211, 484
Mox20I TGGCCA 1 cut(s) 308
MscI TGGCCA 1 cut(s) 308
MseI TTAA 4 cut(s) 213, 321, 408, 750
MslI CAYNNNNRTG 4 cut(s) 393, 395, 466, 654
Msp20I TGGCCA 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 485
MunI CAATTG 1 cut(s) 348
MwoI GCNNNNNNNGC 6 cut(s) 70, 183, 281, 353, 418, 482
NdeI CATATG 2 cut(s) 12, 463
NlaIII CATG 1 cut(s) 702
NlaIV GGNNCC 1 cut(s) 650
NmuCI GTSAC 3 cut(s) 31, 244, 397
OliI CACNNNNGTG 1 cut(s) 654
PceI AGGCCT 1 cut(s) 73
PfeI GAWTC 2 cut(s) 223, 385
PkrI GCNGC 3 cut(s) 253, 274, 556
PleI GAGTC 2 cut(s) 108, 194
PpsI GAGTC 2 cut(s) 108, 194
PshAI GACNNNNGTC 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 650
PspPI GGNCC 3 cut(s) 185, 542, 706
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 527
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 4 cut(s) 393, 395, 466, 654
SaqAI TTAA 4 cut(s) 213, 321, 408, 750
SatI GCNGC 3 cut(s) 252, 273, 555
Sau96I GGNCC 3 cut(s) 185, 542, 706
SchI GAGTC 2 cut(s) 108, 194
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 2 cut(s) 32, 56
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 4 cut(s) 393, 395, 466, 654
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 4 cut(s) 108, 267, 348, 612
SseBI AGGCCT 1 cut(s) 73
StuI AGGCCT 1 cut(s) 73
StyI CCWWGG 2 cut(s) 68, 188
TaaI ACNGT 2 cut(s) 166, 299
TaiI ACGT 2 cut(s) 213, 260
TaqI TCGA 1 cut(s) 112
TasI AATT 4 cut(s) 108, 267, 348, 612
TatI WGTACW 1 cut(s) 228
TfiI GAWTC 2 cut(s) 223, 385
Tru1I TTAA 4 cut(s) 213, 321, 408, 750
Tru9I TTAA 4 cut(s) 213, 321, 408, 750
TscAI CASTG 1 cut(s) 316
TseFI GTSAC 3 cut(s) 31, 244, 397
TseI GCWGC 3 cut(s) 251, 272, 554
Tsp45I GTSAC 3 cut(s) 31, 244, 397
TspDTI ATGAA 3 cut(s) 78, 328, 687
TspRI CASTG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 542
XapI RAATTY 1 cut(s) 612
XcmI CCANNNNNNNNNTGG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.