Rw4G024850

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013259
Physical Location & Seq
Reverse (-)
50784026 .. 50786071
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G024850.1

Sequence Viewer

Length: 726 bp
ATGCCTAATGTGAAATTGTCGGAGGAGCATGCCCAACATCACTATTTGTTAGCAAAGGGCGTCTATGGCCCTTGGTCTGTTGACTCCACCGACGTTAAAGAGGTGATTCTGTACAGGTCAGGATTGGTGACTGCAGCTACGTCATTTGTAATTGCAGCTTCTACTGCTTTTTTGCCCGACAGTTTCTTGGCCACTGAGATTGTTAAGCCCAATCTTGATTACATATACGCAATTGGAGCCGGTGGGTTGGGTTTGTCGTTGGTTCTGATTCACATTTATGTGACTGAGATTAAGCGCACTTTGCAAGCGTTTTGGGGGCTTGGTGTTGTTGGGTCTTTGGCTACATATGTATGCTTGGCTCAACCAGCTGGAGAGGGCTTGGTTCAGTATGTTGTTGATAACCCAGTTGCTGTTTGGTTTGTTGGTCCTTTGTTTGCAGCGCTCACAGGACTGGTTTTCAAGGAAGGTCTTTGCTATGGAAAGTTGGAAGCTGGAATTCTCACATTCATTATACCCATTGTTCTTCTTGGGCACCTGAGTGGGTTGATGGATGATGGAGTGAAACTTTCTCTACTGGGTTCATGGATGGCCCTGTTTGTAATATTTGCTGGCAGGAAGTTCACACAACCCATTAAGGATGACATCGGTGACAAGTCTGTCTTCATATTCCAGTCTCTGCCCGAAGACGAGAAGAAGGCCTTGGTTAAGAAGCTTGAGCAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

241

Amino Acids

26.12

Weight (kDa)

5.71

Isoelectric Point (pI)

29.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 85 - 223 6.7e-55 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 531
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 495
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 113
AfeI AGCGCT 1 cut(s) 441
AgsI TTSAA 1 cut(s) 460
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AleI CACNNNNGTG 1 cut(s) 537
AluBI AGCT 5 cut(s) 137, 158, 368, 491, 712
AluI AGCT 5 cut(s) 137, 158, 368, 491, 712
Alw26I GTCTC 1 cut(s) 678
AlwNI CAGNNNCTG 2 cut(s) 410, 676
Aor51HI AGCGCT 1 cut(s) 441
AoxI GGCC 4 cut(s) 67, 189, 588, 696
ApeKI GCWGC 3 cut(s) 134, 155, 437
ApoI RAATTY 1 cut(s) 495
AspLEI GCGC 2 cut(s) 297, 442
AspS9I GGNCC 3 cut(s) 68, 425, 589
AsuHPI GGTGA 3 cut(s) 115, 139, 659
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 534
BalI TGGCCA 1 cut(s) 191
BanI GGYRCC 1 cut(s) 531
BbsI GAAGAC 2 cut(s) 652, 690
BbvI GCAGC 3 cut(s) 146, 167, 449
BccI CCATC 3 cut(s) 541, 548, 580
BcoDI GTCTC 1 cut(s) 678
BfmI CTRYAG 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 443
BisI GCNGC 3 cut(s) 135, 156, 438
BlsI GCNGC 3 cut(s) 136, 157, 439
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 3 cut(s) 68, 425, 589
BmiI GGNNCC 2 cut(s) 238, 533
BmrI ACTGGG 2 cut(s) 398, 584
BmuI ACTGGG 2 cut(s) 398, 584
BpiI GAAGAC 2 cut(s) 652, 690
BpmI CTGGAG 1 cut(s) 390
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 2 cut(s) 71, 699
Bse118I RCCGGY 1 cut(s) 239
Bse1I ACTGG 4 cut(s) 404, 456, 579, 670
BseDI CCNNGG 2 cut(s) 71, 699
BseGI GGATG 3 cut(s) 556, 591, 643
BseMII CTCAG 3 cut(s) 186, 276, 527
BseNI ACTGG 4 cut(s) 404, 456, 579, 670
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 3 cut(s) 146, 167, 449
BshFI GGCC 4 cut(s) 69, 191, 590, 698
BshNI GGYRCC 1 cut(s) 531
BsiSI CCGG 1 cut(s) 240
BsmAI GTCTC 1 cut(s) 678
BsnI GGCC 4 cut(s) 69, 191, 590, 698
Bsp1286I GDGCHC 1 cut(s) 534
Bsp1407I TGTACA 1 cut(s) 111
BspANI GGCC 4 cut(s) 69, 191, 590, 698
BspCNI CTCAG 3 cut(s) 187, 277, 528
BspLI GGNNCC 2 cut(s) 238, 533
BspMAI CTGCAG 1 cut(s) 136
BspT107I GGYRCC 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 239
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 4 cut(s) 404, 456, 579, 670
BssAI RCCGGY 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 71, 699
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 2 cut(s) 71, 699
Bst4CI ACNGT 2 cut(s) 182, 723
BstACI GRCGYC 1 cut(s) 60
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 30, 306, 610
BstDEI CTNAG 3 cut(s) 195, 285, 536
BstF5I GGATG 3 cut(s) 556, 591, 643
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 2 cut(s) 297, 442
BstMAI GTCTC 1 cut(s) 678
BstMWI GCNNNNNNNGC 5 cut(s) 66, 164, 236, 301, 365
BstNSI RCATGY 1 cut(s) 32
BstSFI CTRYAG 1 cut(s) 132
BstSLI GKGCMC 1 cut(s) 534
BstV1I GCAGC 3 cut(s) 146, 167, 449
BstV2I GAAGAC 2 cut(s) 652, 690
BsuRI GGCC 4 cut(s) 69, 191, 590, 698
BtsCI GGATG 3 cut(s) 556, 591, 643
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 3 cut(s) 30, 306, 610
CaiI CAGNNNCTG 2 cut(s) 410, 676
CfoI GCGC 2 cut(s) 297, 442
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 3 cut(s) 68, 425, 589
CseI GACGC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 112
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 2 cut(s) 29, 582
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 3 cut(s) 195, 285, 536
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
EaeI YGGCCR 1 cut(s) 189
Eco130I CCWWGG 2 cut(s) 71, 699
Eco147I AGGCCT 1 cut(s) 698
Eco47I GGWCC 1 cut(s) 425
Eco47III AGCGCT 1 cut(s) 441
EcoRI GAATTC 1 cut(s) 495
EcoT14I CCWWGG 2 cut(s) 71, 699
ErhI CCWWGG 2 cut(s) 71, 699
FaeI CATG 2 cut(s) 32, 585
FalI AAGNNNNNCTT 4 cut(s) 644, 676, 683, 715
FatI CATG 2 cut(s) 28, 581
FauNDI CATATG 1 cut(s) 346
Fnu4HI GCNGC 3 cut(s) 135, 156, 438
FokI GGATG 3 cut(s) 563, 598, 650
Fsp4HI GCNGC 3 cut(s) 135, 156, 438
GlaI GCGC 2 cut(s) 296, 441
GluI GCNGC 3 cut(s) 135, 156, 438
GsuI CTGGAG 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 4 cut(s) 69, 191, 590, 698
HapII CCGG 1 cut(s) 240
HgaI GACGC 1 cut(s) 49
HhaI GCGC 2 cut(s) 297, 442
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 2 cut(s) 32, 585
Hin6I GCGC 2 cut(s) 295, 440
HinP1I GCGC 2 cut(s) 295, 440
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 3 cut(s) 83, 106, 268
HpaII CCGG 1 cut(s) 240
HphI GGTGA 3 cut(s) 115, 139, 659
Hpy166II GTNNAC 2 cut(s) 82, 621
Hpy188I TCNGA 2 cut(s) 22, 267
Hpy188III TCNNGA 2 cut(s) 120, 215
Hpy8I GTNNAC 2 cut(s) 82, 621
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 458, 688
HpyCH4III ACNGT 2 cut(s) 182, 723
HpyCH4IV ACGT 2 cut(s) 93, 140
HpyCH4V TGCA 4 cut(s) 134, 155, 304, 437
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 164, 236, 301, 365
HpyF3I CTNAG 3 cut(s) 195, 285, 536
HpySE526I ACGT 2 cut(s) 93, 140
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 2 cut(s) 32, 585
HspAI GCGC 2 cut(s) 295, 440
LmnI GCTCC 2 cut(s) 25, 236
Lsp1109I GCAGC 3 cut(s) 146, 167, 449
MaeII ACGT 2 cut(s) 93, 140
MaeIII GTNAC 3 cut(s) 127, 280, 647
MboII GAAGA 4 cut(s) 515, 652, 695, 703
MfeI CAATTG 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 534
MlsI TGGCCA 1 cut(s) 191
MluCI AATT 4 cut(s) 14, 150, 231, 495
MluNI TGGCCA 1 cut(s) 191
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 465
MnlI CCTC 3 cut(s) 16, 94, 367
Mox20I TGGCCA 1 cut(s) 191
MscI TGGCCA 1 cut(s) 191
MseI TTAA 5 cut(s) 96, 204, 291, 633, 705
MslI CAYNNNNRTG 4 cut(s) 276, 278, 349, 537
Msp20I TGGCCA 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 1 cut(s) 240
MunI CAATTG 1 cut(s) 231
MwoI GCNNNNNNNGC 5 cut(s) 66, 164, 236, 301, 365
NdeI CATATG 1 cut(s) 346
NlaIII CATG 2 cut(s) 32, 585
NlaIV GGNNCC 2 cut(s) 238, 533
NmuCI GTSAC 3 cut(s) 127, 280, 647
NspI RCATGY 1 cut(s) 32
OliI CACNNNNGTG 1 cut(s) 537
PaeI GCATGC 1 cut(s) 32
PceI AGGCCT 1 cut(s) 698
PfeI GAWTC 2 cut(s) 106, 268
PkrI GCNGC 3 cut(s) 136, 157, 439
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 238, 533
PspPI GGNCC 3 cut(s) 68, 425, 589
PstI CTGCAG 1 cut(s) 136
PstNI CAGNNNCTG 2 cut(s) 410, 676
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 4 cut(s) 276, 278, 349, 537
SaqAI TTAA 5 cut(s) 96, 204, 291, 633, 705
SatI GCNGC 3 cut(s) 135, 156, 438
Sau96I GGNCC 3 cut(s) 68, 425, 589
SchI GAGTC 1 cut(s) 77
SduI GDGCHC 1 cut(s) 534
SfcI CTRYAG 1 cut(s) 132
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 4 cut(s) 276, 278, 349, 537
SmlI CTYRAG 1 cut(s) 713
SmoI CTYRAG 1 cut(s) 713
SphI GCATGC 1 cut(s) 32
Sse9I AATT 4 cut(s) 14, 150, 231, 495
SseBI AGGCCT 1 cut(s) 698
SspI AATATT 1 cut(s) 603
StuI AGGCCT 1 cut(s) 698
StyI CCWWGG 2 cut(s) 71, 699
TaaI ACNGT 2 cut(s) 182, 723
TaiI ACGT 2 cut(s) 96, 143
TasI AATT 4 cut(s) 14, 150, 231, 495
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 2 cut(s) 106, 268
Tru1I TTAA 5 cut(s) 96, 204, 291, 633, 705
Tru9I TTAA 5 cut(s) 96, 204, 291, 633, 705
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 3 cut(s) 127, 280, 647
TseI GCWGC 3 cut(s) 134, 155, 437
Tsp45I GTSAC 3 cut(s) 127, 280, 647
TspDTI ATGAA 3 cut(s) 496, 570, 652
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 1 cut(s) 495
XceI RCATGY 1 cut(s) 32
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.