Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007916

Physical Location & Seq

Genomic Coordinates Reverse (-)

9920219 .. 9924488

Gene Structure 4270 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G28320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGGATGTGTCTAAAGTTGTCAGCTTTTCCCGGAATTTCGCCGTCTTGGTCAAAGTCGAAGGCCCTGATCCAAAGGGATTGAAGATGAGGAAGCATGCTTTTCATCAATACCATTCTGGCAATGCAACACTTTCAGCTTCTGGGATCTTGCTTCCAAGAGATATTTTTTTGAGTGGTGAGGTCGCTGCTAAGGTTCTGTTTGAAGCTGGTCAGGACATGGCCCTGGTTTTGACCGTTGCATCTGTTGTTGAGCCATTCTTAACATTAGGTCACAGAACTAGTAGTAGTATCTCTCAGGATCCGGTGAAGCTTATTCCGGGTGCTATGATTGAGATTATGGTGGAGGGTCAGCTAAAGTCAGAAAAGGAAGCTCCTTTTTGGGTTCCTGCGCAACTTCTTTCGCTGGTTGATGTCCCTGTATCTTCTGCTGCACTTCAATCTCTTATTGAAGCTTCTTCTGGTTCGAAAGATTCTGGATGGGATATTGGTTGGTCTTTGGTTTCTGCTGCTAATGGTTCTCAGCCTTCAATCAATATTGAACATTACAGCAAGCCCTTGATGCAACTTGATGAGCCACACAACGCTAACTTCATGGCCAAGTCTGCCACTCGAATGGCTATTCTAGGAGTCCCCTTAAGTTTACTGGGTCAACCAAGCATGAATTTTGCTTCATCAAGTAGCAAGGGTGATACACTTGTAGCACTAGGCTCTCCCTTTGGAATCCTTTCACCTGTTAACTTTTTTAACAGCGTATCAACTGGTTCCATTGCGAATAGCTATCCTTCTGGATCCCTAAAGAAGTCACTGATGATAGCTGATGTTCGATGTCTCCCTGGAATGGAAGGGGCTCCGGTGTTTGCTAAGAATGGGCACTTAATTGGCATTTTGATTAGACCACTAAGGCAAAAGAATAGCGGCGTTGAAATTCAGCTGGTGGTTCCATGGGGAGCAATCACAACTGCTTGCAGCCACTTGCTGCTTGAGGAACCATCTGTAGAAGGAAAAGCAAGTCAGTGGGGGAGCGAAGTCCTAAGTGTTAAATCAGATGCTAGTATTCCTGCACAAGTGGCTATTGAGAAGGCGATGGAATCAGTTTGTCTTATTACGGTCAATGATGGTGTTTGGGCTTCTGGTATTATTCTTAACGAACATGGTCTCATACTAACAAATGCTCACCTGCTTGAGCCGTGGAGGTATGGAAAAGGTGGTGTATATGGTGAAGGATTTAAACCCTATGTCTTAGGAGCCGAGGAATTTTCTTCCACGGGAAGTAAATTTTGGGAACAGAAGAGTCAAACATTGCCACGGAAAGCTCCACGAAATCATTATTCGTCTGTTGGAGAGAACATCAGGGAATACAAACACAATTTCCTTCAGACTGGGCATAGAGACATACGTGTGCGTTTGTGTCACCTAGATTCTTGGACTTGGTGTCCTGCAAACGTGGTCTATATTTGCAAGGAACAATTAGATATTGCCTTACTGCAGCTAGAATATGTCCCTGGAAAGCTCCAACCTATTACTGCCAATTTTTCTTCTCCTCCTTTGGGTACAACAGCGCATGTTGTTGGACATGGACTCTTCGGACCAAGATGTGGGCTTTCTCCTTCTATTTGTTCTGGAGTTGTAGCAAAGGTAGTTCACGCAAAGAGGAGATTGAATACGCAATCCATTTCTCAAGAAGTCGCAGAGTTCCCCGCTATGCTCGAAACAACAGCTGCTGTGCATCCTGGTGGCAGTGGTGGTGCTGTTCTCAATTCAAGTGGCCATATGATTGGACTTGTTACCAGCAACGCAAGACATGGAGCGGGGACTGTTATACCGCATCTCAACTTCAGCATCCCATGTGCAGTCTTGGCACCAATCTTCAAGTTTGCAGAAGATATGCAGAACACAACGATCCTTCAAACACTTGATCAACCTAGCGAAGAACTCTCATCCATTTGGGCTTTGATGCCATCACTATCACCAAAGACTGAGCAGTCTCTACCCAATCTGCCTAAGTTACTCAAAGATGGTAATAACAAACAAACGAAAGGATCTCAGTTTGCCAAGTTCATTGCAGAGACCCAAGACATGTTTGTAAAGCCGACCAAGCTTTCCCGTGATGTGATCCCTAGCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0010467 GO:0016485 GO:0016787 GO:0017171 GO:0019538 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051604 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Trypsin_2

Protein Analysis

709

Amino Acids

76.12

Weight (kDa)

8.0

Isoelectric Point (pI)

42.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	191 - 295	2.2e-08	Trypsin-like peptidase domain
Trypsin_2	PF13365	376 - 594	3.3e-18	Trypsin-like peptidase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007916)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28320
fragaria_vesca	FvH4_3g08860 FvH4_3g08860 FvH4_3g08860
malus_domestica	MD10G1291600.v1.1
prunus_persica	Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1
pyrus_communis	pycom10g24380
rosa_chinensis	RchiOBHm_Chr5g0008891 RchiOBHm_Chr5g0008901
rosa_laevigata	RLG00000022692 RLG00000031614
rosa_multiflora	Rmu_co8519179.1_g000001 Rmu_sc0004677.1_g000010
rosa_roxburghii	Rroxscaffold_1G00067110 Rroxscaffold_1G00067730
rosa_rugosa	Rorug04G0440000 Rorug04G0440000
rosa_samantha	Rh5AG068700 Rh5BG065400 Rh5CG076600 Rh5DG064400
rosa_wichuraiana	Rw5G006430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1185
AasI	GACNNNNNNGTC	1 cut(s)	1981
Acc16I	TGCGCA	1 cut(s)	390
Acc36I	ACCTGC	1 cut(s)	1185
AccB1I	GGYRCC	1 cut(s)	1858
AccB7I	CCANNNNNTGG	1 cut(s)	1595
AccBSI	CCGCTC	1 cut(s)	1808
AciI	CCGC	4 cut(s)	915, 1698, 1808, 1823
AclWI	GGATC	9 cut(s)	62, 152, 293, 306, 783, 796, 1894, 2047, 2107
AcoI	YGGCCR	2 cut(s)	594, 1765
AcsI	RAATTY	5 cut(s)	34, 661, 924, 1253, 1274
AcuI	CTGAAG	2 cut(s)	1358, 1819
AfaI	GTAC	1 cut(s)	1552
AfiI	CCNNNNNNNGG	3 cut(s)	838, 1547, 1595
AflII	CTTAAG	1 cut(s)	634
AflIII	ACRYGT	2 cut(s)	1396, 2076
AhdI	GACNNNNNGTC	1 cut(s)	1431
AhlI	ACTAGT	1 cut(s)	278
AjnI	CCWGG	4 cut(s)	222, 832, 1501, 1729
AjuI	GAANNNNNNNTTGG	2 cut(s)	1261, 1293
Alw26I	GTCTC	5 cut(s)	833, 1160, 1383, 1989, 2060
AlwI	GGATC	9 cut(s)	62, 152, 293, 306, 783, 796, 1894, 2047, 2107
AlwNI	CAGNNNCTG	2 cut(s)	140, 1721
AoxI	GGCC	4 cut(s)	61, 219, 594, 1765
ApeKI	GCWGC	7 cut(s)	185, 428, 506, 966, 976, 1486, 1718
ApoI	RAATTY	5 cut(s)	34, 661, 924, 1253, 1274
Asp700I	GAANNNNTTC	1 cut(s)	724
AspLEI	GCGC	2 cut(s)	391, 1561
AspS9I	GGNCC	3 cut(s)	62, 220, 1586
AsuC2I	CCSGG	2 cut(s)	31, 318
AsuHPI	GGTGA	8 cut(s)	188, 316, 698, 720, 1166, 1229, 1403, 1959
AsuII	TTCGAA	1 cut(s)	464
AvaII	GGWCC	1 cut(s)	1586
BaeGI	GKGCMC	1 cut(s)	873
BaeI	ACNNNNGTAYC	2 cut(s)	681, 714
BalI	TGGCCA	2 cut(s)	596, 1767
BamHI	GGATCC	2 cut(s)	298, 788
BanI	GGYRCC	1 cut(s)	1858
BanII	GRGCYC	1 cut(s)	850
BbvI	GCAGC	7 cut(s)	172, 415, 493, 963, 978, 1498, 1705
BccI	CCATC	6 cut(s)	471, 997, 1078, 1109, 1966, 2009
BceAI	ACGGC	2 cut(s)	26, 1171
BciT130I	CCWGG	4 cut(s)	224, 834, 1503, 1731
BclI	TGATCA	1 cut(s)	1915
BcnI	CCSGG	2 cut(s)	31, 318
BcoDI	GTCTC	5 cut(s)	833, 1160, 1383, 1989, 2060
BcuI	ACTAGT	1 cut(s)	278
BfaI	CTAG	8 cut(s)	279, 623, 704, 1050, 1415, 1490, 1923, 2118
BfmI	CTRYAG	2 cut(s)	993, 1484
BfrI	CTTAAG	1 cut(s)	634
BfuAI	ACCTGC	1 cut(s)	1185
BisI	GCNGC	8 cut(s)	186, 429, 507, 916, 967, 977, 1487, 1719
BlsI	GCNGC	8 cut(s)	187, 430, 508, 917, 968, 978, 1488, 1720
Bme1390I	CCNGG	6 cut(s)	31, 224, 318, 834, 1503, 1731
Bme18I	GGWCC	1 cut(s)	1586
BmeRI	GACNNNNNGTC	1 cut(s)	1431
BmgT120I	GGNCC	3 cut(s)	62, 220, 1586
BmiI	GGNNCC	9 cut(s)	300, 384, 763, 790, 849, 939, 987, 1246, 1860
BmrFI	CCNGG	6 cut(s)	31, 224, 318, 834, 1503, 1731
BmrI	ACTGGG	2 cut(s)	653, 1389
BmsI	GCATC	7 cut(s)	248, 549, 1036, 1735, 1834, 1848, 1944
BmuI	ACTGGG	2 cut(s)	653, 1389
BpmI	CTGGAG	1 cut(s)	1641
Bpu10I	CCTNAGC	1 cut(s)	189
Bpu14I	TTCGAA	1 cut(s)	464
BpuEI	CTTGAG	3 cut(s)	1001, 1202, 1662
BpuMI	CCSGG	2 cut(s)	31, 318
BsaAI	YACGTR	1 cut(s)	1397
BsaI	GGTCTC	2 cut(s)	1160, 2060
BsaJI	CCNNGG	8 cut(s)	222, 832, 941, 1187, 1248, 1263, 1304, 1501
BsaWI	WCCGGW	2 cut(s)	301, 850
Bsc4I	CCNNNNNNNGG	3 cut(s)	838, 1547, 1595
Bse1I	ACTGG	3 cut(s)	648, 763, 1384
Bse3DI	GCAATG	4 cut(s)	127, 765, 1298, 2058
BseBI	CCWGG	4 cut(s)	224, 834, 1503, 1731
BseDI	CCNNGG	8 cut(s)	222, 832, 941, 1187, 1248, 1263, 1304, 1501
BseGI	GGATG	5 cut(s)	10, 482, 1726, 1839, 1937
BseLI	CCNNNNNNNGG	3 cut(s)	838, 1547, 1595
BseMI	GCAATG	4 cut(s)	127, 765, 1298, 2058
BseMII	CTCAG	4 cut(s)	308, 533, 1968, 2057
BseNI	ACTGG	3 cut(s)	648, 763, 1384
BseRI	GAGGAG	2 cut(s)	1530, 1666
BseSI	GKGCMC	1 cut(s)	873
BseXI	GCAGC	7 cut(s)	172, 415, 493, 963, 978, 1498, 1705
BsgI	GTGCAG	3 cut(s)	414, 1044, 1869
BshFI	GGCC	4 cut(s)	63, 221, 596, 1767
BshNI	GGYRCC	1 cut(s)	1858
BsiSI	CCGG	4 cut(s)	31, 302, 317, 851
BslFI	GGGAC	4 cut(s)	398, 614, 1484, 1825
BslI	CCNNNNNNNGG	3 cut(s)	838, 1547, 1595
BsmAI	GTCTC	5 cut(s)	833, 1160, 1383, 1989, 2060
BsmFI	GGGAC	4 cut(s)	398, 614, 1484, 1825
BsnI	GGCC	4 cut(s)	63, 221, 596, 1767
Bso31I	GGTCTC	2 cut(s)	1160, 2060
Bsp119I	TTCGAA	1 cut(s)	464
Bsp1286I	GDGCHC	2 cut(s)	850, 873
Bsp143I	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
Bsp19I	CCATGG	1 cut(s)	941
BspACI	CCGC	4 cut(s)	915, 1698, 1808, 1823
BspANI	GGCC	4 cut(s)	63, 221, 596, 1767
BspCNI	CTCAG	4 cut(s)	307, 532, 1969, 2056
BspLI	GGNNCC	9 cut(s)	300, 384, 763, 790, 849, 939, 987, 1246, 1860
BspMAI	CTGCAG	1 cut(s)	1488
BspMI	ACCTGC	1 cut(s)	1185
BspPI	GGATC	9 cut(s)	62, 152, 293, 306, 783, 796, 1894, 2047, 2107
BspT104I	TTCGAA	1 cut(s)	464
BspT107I	GGYRCC	1 cut(s)	1858
BspTI	CTTAAG	1 cut(s)	634
BspTNI	GGTCTC	2 cut(s)	1160, 2060
BsrBI	CCGCTC	1 cut(s)	1808
BsrDI	GCAATG	4 cut(s)	127, 765, 1298, 2058
BsrI	ACTGG	3 cut(s)	648, 763, 1384
BssECI	CCNNGG	8 cut(s)	222, 832, 941, 1187, 1248, 1263, 1304, 1501
BssMI	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
BssT1I	CCWWGG	1 cut(s)	941
Bst2UI	CCWGG	4 cut(s)	224, 834, 1503, 1731
Bst4CI	ACNGT	3 cut(s)	235, 1108, 1816
Bst6I	CTCTTC	2 cut(s)	1283, 1586
BstAFI	CTTAAG	1 cut(s)	634
BstBAI	YACGTR	1 cut(s)	1397
BstBI	TTCGAA	1 cut(s)	464
BstC8I	GCNNGC	3 cut(s)	96, 551, 964
BstDSI	CCRYGG	4 cut(s)	941, 1187, 1263, 1304
BstF5I	GGATG	5 cut(s)	10, 482, 1726, 1839, 1937
BstHHI	GCGC	2 cut(s)	391, 1561
BstKTI	GATC	8 cut(s)	70, 147, 301, 791, 1902, 1918, 2042, 2115
BstMAI	GTCTC	5 cut(s)	833, 1160, 1383, 1989, 2060
BstMBI	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
BstMWI	GCNNNNNNNGC	5 cut(s)	559, 602, 1067, 1856, 2095
BstNI	CCWGG	4 cut(s)	224, 834, 1503, 1731
BstNSI	RCATGY	3 cut(s)	98, 1565, 2080
BstSCI	CCNGG	6 cut(s)	29, 222, 316, 832, 1501, 1729
BstSFI	CTRYAG	2 cut(s)	993, 1484
BstSLI	GKGCMC	1 cut(s)	873
BstV1I	GCAGC	7 cut(s)	172, 415, 493, 963, 978, 1498, 1705
BstX2I	RGATCY	4 cut(s)	144, 298, 788, 2039
BstXI	CCANNNNNNTGG	2 cut(s)	613, 1775
BstYI	RGATCY	4 cut(s)	144, 298, 788, 2039
BsuRI	GGCC	4 cut(s)	63, 221, 596, 1767
BtgI	CCRYGG	4 cut(s)	941, 1187, 1263, 1304
BtgZI	GCGATG	1 cut(s)	1097
BtsCI	GGATG	5 cut(s)	10, 482, 1726, 1839, 1937
BtsI	GCAGTG	1 cut(s)	1744
BtsIMutI	CAGTG	3 cut(s)	803, 1019, 1744
BveI	ACCTGC	1 cut(s)	1185
Cac8I	GCNNGC	3 cut(s)	96, 551, 964
CaiI	CAGNNNCTG	2 cut(s)	140, 1721
CfoI	GCGC	2 cut(s)	391, 1561
Cfr13I	GGNCC	3 cut(s)	62, 220, 1586
Csp6I	GTAC	1 cut(s)	1551
CspCI	CAANNNNNGTGG	2 cut(s)	1744, 1779
CviQI	GTAC	1 cut(s)	1551
DpnI	GATC	8 cut(s)	69, 146, 300, 790, 1901, 1917, 2041, 2114
DpnII	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
DraI	TTTAAA	1 cut(s)	1228
DrdI	GACNNNNNNGTC	1 cut(s)	1981
DriI	GACNNNNNGTC	1 cut(s)	1431
DseDI	GACNNNNNNGTC	1 cut(s)	1981
EaeI	YGGCCR	2 cut(s)	594, 1765
Eam1104I	CTCTTC	2 cut(s)	1283, 1586
Eam1105I	GACNNNNNGTC	1 cut(s)	1431
EarI	CTCTTC	2 cut(s)	1283, 1586
Eco130I	CCWWGG	1 cut(s)	941
Eco24I	GRGCYC	1 cut(s)	850
Eco31I	GGTCTC	2 cut(s)	1160, 2060
Eco47I	GGWCC	1 cut(s)	1586
Eco57I	CTGAAG	2 cut(s)	1358, 1819
EcoO109I	RGGNCCY	1 cut(s)	62
EcoRII	CCWGG	4 cut(s)	222, 832, 1501, 1729
EcoT14I	CCWWGG	1 cut(s)	941
EcoT38I	GRGCYC	1 cut(s)	850
ErhI	CCWWGG	1 cut(s)	941
FaqI	GGGAC	4 cut(s)	398, 614, 1484, 1825
FauI	CCCGC	2 cut(s)	1705, 1801
FauNDI	CATATG	1 cut(s)	1770
FbaI	TGATCA	1 cut(s)	1915
Fnu4HI	GCNGC	8 cut(s)	186, 429, 507, 916, 967, 977, 1487, 1719
FokI	GGATG	5 cut(s)	17, 489, 1713, 1826, 1924
FriOI	GRGCYC	1 cut(s)	850
Fsp4HI	GCNGC	8 cut(s)	186, 429, 507, 916, 967, 977, 1487, 1719
FspBI	CTAG	8 cut(s)	279, 623, 704, 1050, 1415, 1490, 1923, 2118
FspI	TGCGCA	1 cut(s)	390
GlaI	GCGC	2 cut(s)	390, 1560
GluI	GCNGC	8 cut(s)	186, 429, 507, 916, 967, 977, 1487, 1719
GsuI	CTGGAG	1 cut(s)	1641
HaeIII	GGCC	4 cut(s)	63, 221, 596, 1767
HapII	CCGG	4 cut(s)	31, 302, 317, 851
HhaI	GCGC	2 cut(s)	391, 1561
Hin6I	GCGC	2 cut(s)	389, 1559
HinP1I	GCGC	2 cut(s)	389, 1559
HincII	GTYRAC	2 cut(s)	650, 736
HindII	GTYRAC	2 cut(s)	650, 736
HindIII	AAGCTT	3 cut(s)	308, 450, 2096
HinfI	GANTC	7 cut(s)	470, 627, 720, 1088, 1291, 1418, 1578
HpaI	GTTAAC	1 cut(s)	736
HpaII	CCGG	4 cut(s)	31, 302, 317, 851
HphI	GGTGA	8 cut(s)	188, 316, 698, 720, 1166, 1229, 1403, 1959
Hpy166II	GTNNAC	4 cut(s)	641, 650, 736, 1642
Hpy188I	TCNGA	4 cut(s)	361, 1045, 1377, 1586
Hpy188III	TCNNGA	6 cut(s)	212, 296, 474, 786, 1620, 1679
Hpy8I	GTNNAC	4 cut(s)	641, 650, 736, 1642
HpyCH4III	ACNGT	3 cut(s)	235, 1108, 1816
HpyCH4IV	ACGT	2 cut(s)	1396, 1443
HpyF10VI	GCNNNNNNNGC	5 cut(s)	559, 602, 1067, 1856, 2095
HpySE526I	ACGT	2 cut(s)	1396, 1443
HspAI	GCGC	2 cut(s)	389, 1559
Ksp22I	TGATCA	1 cut(s)	1915
KspAI	GTTAAC	1 cut(s)	736
Kzo9I	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
LmnI	GCTCC	8 cut(s)	376, 853, 947, 1020, 1244, 1318, 1515, 1805
Lsp1109I	GCAGC	7 cut(s)	172, 415, 493, 963, 978, 1498, 1705
LweI	GCATC	7 cut(s)	248, 549, 1036, 1735, 1834, 1848, 1944
MaeI	CTAG	8 cut(s)	279, 623, 704, 1050, 1415, 1490, 1923, 2118
MaeII	ACGT	2 cut(s)	1396, 1443
MaeIII	GTNAC	5 cut(s)	269, 801, 1409, 1783, 2004
MalI	GATC	8 cut(s)	69, 146, 300, 790, 1901, 1917, 2041, 2114
MbiI	CCGCTC	1 cut(s)	1808
MboI	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
MflI	RGATCY	4 cut(s)	144, 298, 788, 2039
MhlI	GDGCHC	2 cut(s)	850, 873
MlsI	TGGCCA	2 cut(s)	596, 1767
MluNI	TGGCCA	2 cut(s)	596, 1767
MlyI	GAGTC	3 cut(s)	636, 1300, 1572
MmeI	TCCRAC	3 cut(s)	1318, 1537, 1549
MnlI	CCTC	8 cut(s)	81, 172, 337, 976, 1185, 1243, 1551, 1644
Mox20I	TGGCCA	2 cut(s)	596, 1767
MroXI	GAANNNNTTC	1 cut(s)	724
MscI	TGGCCA	2 cut(s)	596, 1767
MseI	TTAA	8 cut(s)	260, 635, 735, 744, 875, 1038, 1143, 1227
MslI	CAYNNNNRTG	3 cut(s)	611, 1397, 1731
Msp20I	TGGCCA	2 cut(s)	596, 1767
MspA1I	CMGCKG	2 cut(s)	931, 1718
MspCI	CTTAAG	1 cut(s)	634
MspI	CCGG	4 cut(s)	31, 302, 317, 851
MspR9I	CCNGG	6 cut(s)	31, 224, 318, 834, 1503, 1731
MvaI	CCWGG	4 cut(s)	224, 834, 1503, 1731
MwoI	GCNNNNNNNGC	5 cut(s)	559, 602, 1067, 1856, 2095
NciI	CCSGG	2 cut(s)	31, 318
NcoI	CCATGG	1 cut(s)	941
NdeI	CATATG	1 cut(s)	1770
NdeII	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
NlaIV	GGNNCC	9 cut(s)	300, 384, 763, 790, 849, 939, 987, 1246, 1860
NmeAIII	GCCGAG	1 cut(s)	1273
NmuCI	GTSAC	3 cut(s)	269, 801, 1409
NsbI	TGCGCA	1 cut(s)	390
NspI	RCATGY	3 cut(s)	98, 1565, 2080
NspV	TTCGAA	1 cut(s)	464
PaeI	GCATGC	1 cut(s)	98
PaqCI	CACCTGC	1 cut(s)	1185
PciI	ACATGT	1 cut(s)	2076
PdmI	GAANNNNTTC	1 cut(s)	724
PfeI	GAWTC	4 cut(s)	470, 720, 1088, 1418
PflMI	CCANNNNNTGG	1 cut(s)	1595
PfoI	TCCNGGA	1 cut(s)	29
PkrI	GCNGC	8 cut(s)	187, 430, 508, 917, 968, 978, 1488, 1720
PleI	GAGTC	3 cut(s)	635, 1299, 1572
PpsI	GAGTC	3 cut(s)	635, 1299, 1572
Ppu21I	YACGTR	1 cut(s)	1397
PscI	ACATGT	1 cut(s)	2076
Psp6I	CCWGG	4 cut(s)	222, 832, 1501, 1729
PspGI	CCWGG	4 cut(s)	222, 832, 1501, 1729
PspN4I	GGNNCC	9 cut(s)	300, 384, 763, 790, 849, 939, 987, 1246, 1860
PspPI	GGNCC	3 cut(s)	62, 220, 1586
PstI	CTGCAG	1 cut(s)	1488
PstNI	CAGNNNCTG	2 cut(s)	140, 1721
PsuI	RGATCY	4 cut(s)	144, 298, 788, 2039
PvuII	CAGCTG	2 cut(s)	931, 1718
RsaI	GTAC	1 cut(s)	1552
RsaNI	GTAC	1 cut(s)	1551
RseI	CAYNNNNRTG	3 cut(s)	611, 1397, 1731
SaqAI	TTAA	8 cut(s)	260, 635, 735, 744, 875, 1038, 1143, 1227
SatI	GCNGC	8 cut(s)	186, 429, 507, 916, 967, 977, 1487, 1719
Sau3AI	GATC	8 cut(s)	67, 144, 298, 788, 1899, 1915, 2039, 2112
Sau96I	GGNCC	3 cut(s)	62, 220, 1586
SchI	GAGTC	3 cut(s)	636, 1300, 1572
ScrFI	CCNGG	6 cut(s)	31, 224, 318, 834, 1503, 1731
SduI	GDGCHC	2 cut(s)	850, 873
SfaNI	GCATC	7 cut(s)	248, 549, 1036, 1735, 1834, 1848, 1944
SfcI	CTRYAG	2 cut(s)	993, 1484
SfuI	TTCGAA	1 cut(s)	464
SinI	GGWCC	1 cut(s)	1586
SmiMI	CAYNNNNRTG	3 cut(s)	611, 1397, 1731
SmlI	CTYRAG	4 cut(s)	634, 980, 1181, 1677
SmoI	CTYRAG	4 cut(s)	634, 980, 1181, 1677
SpeI	ACTAGT	1 cut(s)	278
SphI	GCATGC	1 cut(s)	98
SsiI	CCGC	4 cut(s)	915, 1698, 1808, 1823
SspI	AATATT	1 cut(s)	535
SspMI	CTAG	8 cut(s)	279, 623, 704, 1050, 1415, 1490, 1923, 2118
StyD4I	CCNGG	6 cut(s)	29, 222, 316, 832, 1501, 1729
StyI	CCWWGG	1 cut(s)	941
TaaI	ACNGT	3 cut(s)	235, 1108, 1816
TaiI	ACGT	2 cut(s)	1399, 1446
TaqI	TCGA	5 cut(s)	57, 464, 610, 823, 1707
TauI	GCSGC	1 cut(s)	918
TfiI	GAWTC	4 cut(s)	470, 720, 1088, 1418
Tru1I	TTAA	8 cut(s)	260, 635, 735, 744, 875, 1038, 1143, 1227
Tru9I	TTAA	8 cut(s)	260, 635, 735, 744, 875, 1038, 1143, 1227
TscAI	CASTG	3 cut(s)	810, 1019, 1744
TseFI	GTSAC	3 cut(s)	269, 801, 1409
TseI	GCWGC	7 cut(s)	185, 428, 506, 966, 976, 1486, 1718
Tsp45I	GTSAC	3 cut(s)	269, 801, 1409
TspDTI	ATGAA	5 cut(s)	92, 580, 660, 674, 2047
TspGWI	ACGGA	1 cut(s)	1321
TspRI	CASTG	3 cut(s)	810, 1019, 1744
Van91I	CCANNNNNTGG	1 cut(s)	1595
Vha464I	CTTAAG	1 cut(s)	634
VpaK11BI	GGWCC	1 cut(s)	1586
XapI	RAATTY	5 cut(s)	34, 661, 924, 1253, 1274
XceI	RCATGY	3 cut(s)	98, 1565, 2080
XmnI	GAANNNNTTC	1 cut(s)	724
XspI	CTAG	8 cut(s)	279, 623, 704, 1050, 1415, 1490, 1923, 2118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.