Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007916

Physical Location & Seq

Genomic Coordinates Forward (+)

38007858 .. 38013573

Gene Structure 5716 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1291600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGGGTCTGCCGGAAATCGTAGATTTCGCGCGCAATTTCGCCGTCATGGTCAGAGTCAAAGGCCCTGATCCAAAGGGCCTCAAAATGCGAAACCACGCTTTCCACCATTACCACTCTGGGACGACGACCATTTCGGCGTCGGGAATGTTATTGCCGGACTCCCTTTACGACTCTGGTGTTGCTAAGCAACTTTTTGGCGGCGGTGGAGACGGTGGAGGCGGTGGGGGCGGCAGCCAACGGTCTCCTGTTGTCGTGGTGACTGTTGCTTCCATTGTTGAGCCTTTTCTCTCTTTGGAGCACAGAAAGGGCCTTGCCCAGGGCAGGCATCAGTTGATTCCTGGGGTTCAGATTGATATTATGGTAGAGGATGAAATGAGGTTGGAGAAAGAATCTCGGGATTCGGATAAAGCACATCCCTGCTGGTTTGCAGCACAGCTTTTAACACTGATTGACGTTCCTGCATCTGCTGCTGCAATCCAATCACTTATTGAAGCTTCTTTAAGCTCCCCCGATCATGGATGGGAGGTCGGTTGGTCTTTAGCCTCACATAGTAATGCTCCTCAGGCCCAGGTGGACTTTGGAGTCAGAGAATTGGGGAATTTAACTCTCACAGGCAAGTCAAATACAAGAATCGCCATTCTTGGAGTATCCCTAATTCCCAAGGACGTGCCAAATATAACCATATCACCCTCCAATCAAAGGGGGGACTTTCTTGTGGCTGTGGGTTCTCCTTTTGGTATCTTGTCAACTTTGCACTTCTTTAACAGTATATCAGTGGGATCCATTGCCAACTGTTATCCTCCTAATTCATCTTCTAGTTCATTGCTGATGGCAGACATACGCTGTCTTCCTGGAGTGGAAGGTGGTCCAGTTTTGAGTGAGCAGGCACAACTTATTGGTATGTTGATTAGACCGTTGAGGCAGAAGACTAGTGGGGCTGAGATTCAGCTGGTGATTTCATGGGAAGCCATTGCAACTGCTTGCAGTGACTTGCTGCGGAAGGAGCCCCATTATGTAGAAAAAGGGATTTCTGTTGACAAGGGAACCTCAGATGATGTAGGGAAGCCAATTTTAGCTGAGAGCCACGGTTCAAATGGAGCCATAACACATATTCAGGAATGCATTTATTCTAGTTGCTCCTCCCCGTCACCAGTTGAGAAGGCAATGGGTTCTGTTTGTCTTATAAACATTGATGATGGAGCATGGGCATCAGGTGTCTTTCTAAACAAGCAAGGTCTCATACTCACAAATGCTCACCTGTTAGAACCATGGAGATTTGGTAAAAGAACTGCAAGTGATGGAAGGCATGGATCCATTTCTGAGGCATTATCTCCTAGGCATAGTGGACTATATGGCAAGCAAAAGAGTGAAGGTTGTCCCCCAATGGTTCACAGTAATGCAGATCCCTCAGTTGGCGATGAACATGGAGGATATGTATTGAGTTCTTACAGAGGTCAGAGAAGGATACGTGTACGCCTAGATCATAGAGACCCTTGGATTTGGTGTGATGCTAAAGTAGTGTATGTTTGCAAGGGACCCGTGGATGTTGCCTTGTTGCAACTCAAGTACATCACTGATCAGCTTTCTCCAATTGTCATGGATTTTATGTCACCATCTGTAGGATCAAAGGCATATGTTATTGGACATGGACTATTAGGACCAAGATGCGGGTTCTCCCCATCAATTTGTTCTGGTGTTGTCGCAAAAGTTTTAAAGTCAAAGTTTCCGCACTCATATCAAACGAACCGAAGTACTCAAGGAGATCTTCCTGCAATGCTTGAAACAACAGCTGCTGTCCATCCTGGTGGTAGTGGTGGCGCTGTCGTTAATTCAGAGGGTCATATGATTGGACTTGTTACAAGTAATGCAAGGCATGGTGGAGGAACAATTATACCACATCTGAACTTCAGCATTCCATGTGCTGCTCTCCTGCCAATCTTCAAGTTCTCAAAAGACATGCAGGATCTGTCACTTCTCCAAGTTCTTGACCAACCAAACGAATATCTTTCTTCCGTATGGGCGTTGATGCCACCTCTGTCTCCCAAGCCCCCTCCTTTGCCGCCTCATATTCCAGAGTCATTACTACAAGATAACAACAAAGAAGCAAAAGGCTCTCGTTTTGCAAGATTTGTCGCCGAAAGAAAAGATGTGTTTGAAAACCCAAACCAACTTGGCAAGGAAAGAAGACTTCCTAACAACCTTGTTCCTAGCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0010467 GO:0016485 GO:0016787 GO:0017171 GO:0019538 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051604 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Trypsin_2

Protein Analysis

740

Amino Acids

79.16

Weight (kDa)

7.36

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	200 - 301	8.1e-08	Trypsin-like peptidase domain
Trypsin_2	PF13365	403 - 618	2e-16	Trypsin-like peptidase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007916)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28320
fragaria_vesca	FvH4_3g08860 FvH4_3g08860 FvH4_3g08860
malus_domestica	MD10G1291600.v1.1
prunus_persica	Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1
pyrus_communis	pycom10g24380
rosa_chinensis	RchiOBHm_Chr5g0008891 RchiOBHm_Chr5g0008901
rosa_laevigata	RLG00000022692 RLG00000031614
rosa_multiflora	Rmu_co8519179.1_g000001 Rmu_sc0004677.1_g000010
rosa_roxburghii	Rroxscaffold_1G00067110 Rroxscaffold_1G00067730
rosa_rugosa	Rorug04G0440000 Rorug04G0440000
rosa_samantha	Rh5AG068700 Rh5BG065400 Rh5CG076600 Rh5DG064400
rosa_wichuraiana	Rw5G006430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1184
AasI	GACNNNNNNGTC	1 cut(s)	581
AccII	CGCG	2 cut(s)	29, 31
AciI	CCGC	8 cut(s)	198, 201, 219, 228, 997, 1668, 1727, 2060
AclWI	GGATC	8 cut(s)	62, 774, 787, 1305, 1318, 1397, 1630, 1971
AcsI	RAATTY	1 cut(s)	598
AcuI	CTGAAG	1 cut(s)	1891
AcyI	GRCGYC	1 cut(s)	137
AfaI	GTAC	3 cut(s)	1473, 1568, 1753
AfiI	CCNNNNNNNGG	7 cut(s)	316, 321, 515, 699, 1412, 1619, 1667
AflIII	ACRYGT	1 cut(s)	1468
AgsI	TTSAA	5 cut(s)	491, 1092, 1781, 1942, 2156
AhlI	ACTAGT	1 cut(s)	929
AjiI	CACGTC	1 cut(s)	667
AjnI	CCWGG	5 cut(s)	315, 337, 567, 850, 1801
AluBI	AGCT	7 cut(s)	436, 494, 504, 949, 1076, 1582, 1790
AluI	AGCT	7 cut(s)	436, 494, 504, 949, 1076, 1582, 1790
Alw21I	GWGCWC	1 cut(s)	300
Alw26I	GTCTC	5 cut(s)	201, 246, 1241, 1482, 2043
AlwI	GGATC	8 cut(s)	62, 774, 787, 1305, 1318, 1397, 1630, 1971
AlwNI	CAGNNNCTG	2 cut(s)	1793, 1966
Ama87I	CYCGRG	1 cut(s)	393
AoxI	GGCC	4 cut(s)	61, 76, 307, 564
ApeKI	GCWGC	7 cut(s)	231, 428, 467, 470, 994, 1790, 1922
ApoI	RAATTY	1 cut(s)	598
ArsI	GACNNNNNNTTYG	2 cut(s)	115, 147
AspA2I	CCTAGG	1 cut(s)	1334
AspLEI	GCGC	3 cut(s)	31, 33, 1820
AspS9I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
AsuHPI	GGTGA	6 cut(s)	268, 678, 964, 1140, 1247, 1602
AvaI	CYCGRG	1 cut(s)	393
AvaII	GGWCC	3 cut(s)	866, 1535, 1658
AvrII	CCTAGG	1 cut(s)	1334
AxyI	CCTNAGG	1 cut(s)	561
BamHI	GGATCC	2 cut(s)	779, 1310
BanII	GRGCYC	1 cut(s)	1008
BbsI	GAAGAC	3 cut(s)	839, 932, 2191
Bbv12I	GWGCWC	1 cut(s)	300
BbvI	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1777, 1909
BccI	CCATC	7 cut(s)	513, 823, 1190, 1292, 1621, 1687, 1806
BceAI	ACGGC	1 cut(s)	26
BciT130I	CCWGG	5 cut(s)	317, 339, 569, 852, 1803
BciVI	GTATCC	2 cut(s)	658, 1458
BclI	TGATCA	1 cut(s)	1576
BcoDI	GTCTC	5 cut(s)	201, 246, 1241, 1482, 2043
BcuI	ACTAGT	1 cut(s)	929
BfaI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2208
BfmI	CTRYAG	1 cut(s)	1617
BfoI	RGCGCY	1 cut(s)	1821
BfuI	GTATCC	2 cut(s)	658, 1458
BglII	AGATCT	1 cut(s)	1762
BlnI	CCTAGG	1 cut(s)	1334
BlpI	GCTNAGC	1 cut(s)	183
BmcAI	AGTACT	1 cut(s)	1753
Bme1390I	CCNGG	5 cut(s)	317, 339, 569, 852, 1803
Bme18I	GGWCC	3 cut(s)	866, 1535, 1658
BmeT110I	CYCGRG	1 cut(s)	393
BmgBI	CACGTC	1 cut(s)	667
BmgT120I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
BmiI	GGNNCC	7 cut(s)	781, 1005, 1045, 1099, 1312, 1536, 1537
BmrFI	CCNGG	5 cut(s)	317, 339, 569, 852, 1803
BmsI	GCATC	6 cut(s)	334, 470, 1217, 1498, 1655, 2016
BpiI	GAAGAC	3 cut(s)	839, 932, 2191
BpmI	CTGGAG	1 cut(s)	873
Bpu1102I	GCTNAGC	1 cut(s)	183
BpuEI	CTTGAG	2 cut(s)	1547, 1740
BsaAI	YACGTR	1 cut(s)	1469
BsaHI	GRCGYC	1 cut(s)	137
BsaI	GGTCTC	3 cut(s)	246, 1241, 1482
BsaXI	ACNNNNNCTCC	4 cut(s)	1264, 1294, 1419, 1449
Bsc4I	CCNNNNNNNGG	7 cut(s)	316, 321, 515, 699, 1412, 1619, 1667
Bse1I	ACTGG	2 cut(s)	869, 1151
Bse21I	CCTNAGG	1 cut(s)	561
Bse3DI	GCAATG	5 cut(s)	783, 821, 969, 1170, 1779
BseBI	CCWGG	5 cut(s)	317, 339, 569, 852, 1803
BseGI	GGATG	5 cut(s)	373, 412, 524, 1549, 1798
BseLI	CCNNNNNNNGG	7 cut(s)	316, 321, 515, 699, 1412, 1619, 1667
BseMI	GCAATG	5 cut(s)	783, 821, 969, 1170, 1779
BseMII	CTCAG	6 cut(s)	575, 930, 1062, 1068, 1311, 1422
BseNI	ACTGG	2 cut(s)	869, 1151
BsePI	GCGCGC	1 cut(s)	29
BseRI	GAGGAG	2 cut(s)	549, 1129
BseXI	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1777, 1909
Bsh1236I	CGCG	2 cut(s)	29, 31
BshFI	GGCC	4 cut(s)	63, 78, 309, 566
BsiHKAI	GWGCWC	1 cut(s)	300
BsiHKCI	CYCGRG	1 cut(s)	393
BsiSI	CCGG	2 cut(s)	11, 155
BslFI	GGGAC	4 cut(s)	133, 719, 1362, 1548
BslI	CCNNNNNNNGG	7 cut(s)	316, 321, 515, 699, 1412, 1619, 1667
BsmAI	GTCTC	5 cut(s)	201, 246, 1241, 1482, 2043
BsmBI	CGTCTC	1 cut(s)	201
BsmFI	GGGAC	4 cut(s)	133, 719, 1362, 1548
BsmI	GAATGC	2 cut(s)	1124, 1911
BsnI	GGCC	4 cut(s)	63, 78, 309, 566
Bso31I	GGTCTC	3 cut(s)	246, 1241, 1482
BsoBI	CYCGRG	1 cut(s)	393
Bsp1286I	GDGCHC	2 cut(s)	300, 1008
Bsp1720I	GCTNAGC	1 cut(s)	183
Bsp19I	CCATGG	1 cut(s)	1268
BspACI	CCGC	8 cut(s)	198, 201, 219, 228, 997, 1668, 1727, 2060
BspANI	GGCC	4 cut(s)	63, 78, 309, 566
BspCNI	CTCAG	6 cut(s)	574, 931, 1061, 1069, 1312, 1421
BspFNI	CGCG	2 cut(s)	29, 31
BspLI	GGNNCC	7 cut(s)	781, 1005, 1045, 1099, 1312, 1536, 1537
BspPI	GGATC	8 cut(s)	62, 774, 787, 1305, 1318, 1397, 1630, 1971
BspTNI	GGTCTC	3 cut(s)	246, 1241, 1482
BsrDI	GCAATG	5 cut(s)	783, 821, 969, 1170, 1779
BsrI	ACTGG	2 cut(s)	869, 1151
BssHII	GCGCGC	1 cut(s)	29
BssNI	GRCGYC	1 cut(s)	137
BssT1I	CCWWGG	4 cut(s)	660, 1268, 1334, 1493
Bst2UI	CCWGG	5 cut(s)	317, 339, 569, 852, 1803
Bst4CI	ACNGT	8 cut(s)	212, 240, 262, 767, 794, 915, 1088, 1394
BstACI	GRCGYC	1 cut(s)	137
BstAPI	GCANNNNNTGC	1 cut(s)	467
BstBAI	YACGTR	1 cut(s)	1469
BstC8I	GCNNGC	5 cut(s)	31, 323, 885, 982, 1358
BstDEI	CTNAG	7 cut(s)	183, 561, 939, 1048, 1077, 1320, 1408
BstDSI	CCRYGG	3 cut(s)	1084, 1268, 1539
BstENI	CCTNNNNNAGG	1 cut(s)	314
BstF5I	GGATG	5 cut(s)	373, 412, 524, 1549, 1798
BstFNI	CGCG	2 cut(s)	29, 31
BstH2I	RGCGCY	1 cut(s)	1821
BstHHI	GCGC	3 cut(s)	31, 33, 1820
BstMAI	GTCTC	5 cut(s)	201, 246, 1241, 1482, 2043
BstMWI	GCNNNNNNNGC	4 cut(s)	225, 467, 563, 1003
BstNI	CCWGG	5 cut(s)	317, 339, 569, 852, 1803
BstNSI	RCATGY	1 cut(s)	1960
BstSCI	CCNGG	5 cut(s)	315, 337, 567, 850, 1801
BstSFI	CTRYAG	1 cut(s)	1617
BstUI	CGCG	2 cut(s)	29, 31
BstV1I	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1777, 1909
BstV2I	GAAGAC	3 cut(s)	839, 932, 2191
BstX2I	RGATCY	5 cut(s)	779, 1310, 1402, 1762, 1963
BstXI	CCANNNNNNTGG	1 cut(s)	1805
BstYI	RGATCY	5 cut(s)	779, 1310, 1402, 1762, 1963
Bsu36I	CCTNAGG	1 cut(s)	561
BsuI	GTATCC	2 cut(s)	658, 1458
BsuRI	GGCC	4 cut(s)	63, 78, 309, 566
BtgI	CCRYGG	3 cut(s)	1084, 1268, 1539
BtgZI	GCGATG	1 cut(s)	1431
BtrI	CACGTC	1 cut(s)	667
BtsCI	GGATG	5 cut(s)	373, 412, 524, 1549, 1798
BtsI	GCAGTG	1 cut(s)	991
BtsIMutI	CAGTG	4 cut(s)	443, 780, 991, 1572
Cac8I	GCNNGC	5 cut(s)	31, 323, 885, 982, 1358
CaiI	CAGNNNCTG	2 cut(s)	1793, 1966
CfoI	GCGC	3 cut(s)	31, 33, 1820
Cfr13I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
CseI	GACGC	1 cut(s)	126
Csp6I	GTAC	3 cut(s)	1472, 1567, 1752
CviQI	GTAC	3 cut(s)	1472, 1567, 1752
DdeI	CTNAG	7 cut(s)	183, 561, 939, 1048, 1077, 1320, 1408
DraI	TTTAAA	1 cut(s)	1713
DrdI	GACNNNNNNGTC	1 cut(s)	581
DseDI	GACNNNNNNGTC	1 cut(s)	581
Eco130I	CCWWGG	4 cut(s)	660, 1268, 1334, 1493
Eco24I	GRGCYC	1 cut(s)	1008
Eco31I	GGTCTC	3 cut(s)	246, 1241, 1482
Eco47I	GGWCC	3 cut(s)	866, 1535, 1658
Eco57I	CTGAAG	1 cut(s)	1891
Eco81I	CCTNAGG	1 cut(s)	561
Eco88I	CYCGRG	1 cut(s)	393
EcoNI	CCTNNNNNAGG	1 cut(s)	314
EcoO109I	RGGNCCY	4 cut(s)	62, 76, 307, 1535
EcoRII	CCWGG	5 cut(s)	315, 337, 567, 850, 1801
EcoT14I	CCWWGG	4 cut(s)	660, 1268, 1334, 1493
EcoT22I	ATGCAT	1 cut(s)	1124
EcoT38I	GRGCYC	1 cut(s)	1008
ErhI	CCWWGG	4 cut(s)	660, 1268, 1334, 1493
Esp3I	CGTCTC	1 cut(s)	201
FalI	AAGNNNNNCTT	2 cut(s)	1749, 1781
FaqI	GGGAC	4 cut(s)	133, 719, 1362, 1548
FauI	CCCGC	1 cut(s)	1661
FauNDI	CATATG	2 cut(s)	1633, 1842
FbaI	TGATCA	1 cut(s)	1576
FokI	GGATG	5 cut(s)	380, 399, 531, 1556, 1785
FriOI	GRGCYC	1 cut(s)	1008
FspBI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2208
GlaI	GCGC	3 cut(s)	30, 32, 1819
GsuI	CTGGAG	1 cut(s)	873
HaeII	RGCGCY	1 cut(s)	1821
HaeIII	GGCC	4 cut(s)	63, 78, 309, 566
HapII	CCGG	2 cut(s)	11, 155
HgaI	GACGC	1 cut(s)	126
HhaI	GCGC	3 cut(s)	31, 33, 1820
Hin1I	GRCGYC	1 cut(s)	137
Hin6I	GCGC	3 cut(s)	29, 31, 1818
HinP1I	GCGC	3 cut(s)	29, 31, 1818
HincII	GTYRAC	2 cut(s)	747, 1036
HindII	GTYRAC	2 cut(s)	747, 1036
HindIII	AAGCTT	1 cut(s)	492
HpaII	CCGG	2 cut(s)	11, 155
HphI	GGTGA	6 cut(s)	268, 678, 964, 1140, 1247, 1602
Hpy166II	GTNNAC	6 cut(s)	574, 747, 1036, 1346, 1390, 1472
Hpy188I	TCNGA	9 cut(s)	53, 348, 403, 587, 1051, 1321, 1458, 1834, 1902
Hpy188III	TCNNGA	5 cut(s)	141, 395, 1115, 1985, 2072
Hpy8I	GTNNAC	6 cut(s)	574, 747, 1036, 1346, 1390, 1472
Hpy99I	CGWCG	2 cut(s)	127, 142
HpyAV	CCTTC	6 cut(s)	854, 994, 1153, 1296, 1364, 1455
HpyCH4III	ACNGT	8 cut(s)	212, 240, 262, 767, 794, 915, 1088, 1394
HpyCH4IV	ACGT	3 cut(s)	453, 666, 1468
HpyF10VI	GCNNNNNNNGC	4 cut(s)	225, 467, 563, 1003
HpyF3I	CTNAG	7 cut(s)	183, 561, 939, 1048, 1077, 1320, 1408
HpySE526I	ACGT	3 cut(s)	453, 666, 1468
Hsp92I	GRCGYC	1 cut(s)	137
HspAI	GCGC	3 cut(s)	29, 31, 1818
KflI	GGGWCCC	1 cut(s)	1535
Ksp22I	TGATCA	1 cut(s)	1576
LmnI	GCTCC	7 cut(s)	295, 509, 562, 1003, 1097, 1142, 1199
Lsp1109I	GCAGC	7 cut(s)	243, 440, 454, 457, 981, 1777, 1909
LweI	GCATC	6 cut(s)	334, 470, 1217, 1498, 1655, 2016
MaeI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2208
MaeII	ACGT	3 cut(s)	453, 666, 1468
MaeIII	GTNAC	6 cut(s)	256, 986, 1146, 1608, 1855, 1968
MboII	GAAGA	7 cut(s)	804, 839, 937, 1757, 1930, 2001, 2196
MfeI	CAATTG	1 cut(s)	1590
MflI	RGATCY	5 cut(s)	779, 1310, 1402, 1762, 1963
MhlI	GDGCHC	2 cut(s)	300, 1008
MlyI	GAGTC	5 cut(s)	63, 152, 164, 591, 2084
MmeI	TCCRAC	1 cut(s)	360
Mph1103I	ATGCAT	1 cut(s)	1124
MseI	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1827
MslI	CAYNNNNRTG	3 cut(s)	552, 1395, 1803
MspA1I	CMGCKG	2 cut(s)	949, 1790
MspI	CCGG	2 cut(s)	11, 155
MspR9I	CCNGG	5 cut(s)	317, 339, 569, 852, 1803
MunI	CAATTG	1 cut(s)	1590
Mva1269I	GAATGC	2 cut(s)	1124, 1911
MvaI	CCWGG	5 cut(s)	317, 339, 569, 852, 1803
MvnI	CGCG	2 cut(s)	29, 31
MwoI	GCNNNNNNNGC	4 cut(s)	225, 467, 563, 1003
NcoI	CCATGG	1 cut(s)	1268
NdeI	CATATG	2 cut(s)	1633, 1842
NlaIV	GGNNCC	7 cut(s)	781, 1005, 1045, 1099, 1312, 1536, 1537
NmuCI	GTSAC	5 cut(s)	256, 986, 1146, 1608, 1968
NsiI	ATGCAT	1 cut(s)	1124
NspI	RCATGY	1 cut(s)	1960
PasI	CCCWGGG	1 cut(s)	316
PauI	GCGCGC	1 cut(s)	29
PctI	GAATGC	2 cut(s)	1124, 1911
PfeI	GAWTC	5 cut(s)	334, 389, 398, 630, 943
PfoI	TCCNGGA	1 cut(s)	850
PleI	GAGTC	5 cut(s)	62, 152, 164, 590, 2083
PpsI	GAGTC	5 cut(s)	62, 152, 164, 590, 2083
Ppu21I	YACGTR	1 cut(s)	1469
PpuMI	RGGWCCY	1 cut(s)	1535
PsiI	TTATAA	1 cut(s)	1184
Psp5II	RGGWCCY	1 cut(s)	1535
Psp6I	CCWGG	5 cut(s)	315, 337, 567, 850, 1801
PspGI	CCWGG	5 cut(s)	315, 337, 567, 850, 1801
PspN4I	GGNNCC	7 cut(s)	781, 1005, 1045, 1099, 1312, 1536, 1537
PspPI	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
PspPPI	RGGWCCY	1 cut(s)	1535
PstNI	CAGNNNCTG	2 cut(s)	1793, 1966
PsuI	RGATCY	5 cut(s)	779, 1310, 1402, 1762, 1963
PteI	GCGCGC	1 cut(s)	29
PvuII	CAGCTG	2 cut(s)	949, 1790
RsaI	GTAC	3 cut(s)	1473, 1568, 1753
RsaNI	GTAC	3 cut(s)	1472, 1567, 1752
RseI	CAYNNNNRTG	3 cut(s)	552, 1395, 1803
SaqAI	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1827
Sau96I	GGNCC	7 cut(s)	62, 76, 307, 565, 866, 1535, 1658
ScaI	AGTACT	1 cut(s)	1753
SchI	GAGTC	5 cut(s)	63, 152, 164, 591, 2084
ScrFI	CCNGG	5 cut(s)	317, 339, 569, 852, 1803
SduI	GDGCHC	2 cut(s)	300, 1008
SfaNI	GCATC	6 cut(s)	334, 470, 1217, 1498, 1655, 2016
SfcI	CTRYAG	1 cut(s)	1617
SinI	GGWCC	3 cut(s)	866, 1535, 1658
SmiMI	CAYNNNNRTG	3 cut(s)	552, 1395, 1803
SmlI	CTYRAG	2 cut(s)	1562, 1755
SmoI	CTYRAG	2 cut(s)	1562, 1755
SpeI	ACTAGT	1 cut(s)	929
SsiI	CCGC	8 cut(s)	198, 201, 219, 228, 997, 1668, 1727, 2060
SspMI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2208
StyD4I	CCNGG	5 cut(s)	315, 337, 567, 850, 1801
StyI	CCWWGG	4 cut(s)	660, 1268, 1334, 1493
TaaI	ACNGT	8 cut(s)	212, 240, 262, 767, 794, 915, 1088, 1394
TaiI	ACGT	3 cut(s)	456, 669, 1471
TatI	WGTACW	2 cut(s)	1566, 1751
TauI	GCSGC	3 cut(s)	201, 231, 2062
TfiI	GAWTC	5 cut(s)	334, 389, 398, 630, 943
Tru1I	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1827
Tru9I	TTAA	6 cut(s)	440, 500, 602, 762, 1712, 1827
TscAI	CASTG	4 cut(s)	450, 780, 991, 1579
TseFI	GTSAC	5 cut(s)	256, 986, 1146, 1608, 1968
TseI	GCWGC	7 cut(s)	231, 428, 467, 470, 994, 1790, 1922
Tsp45I	GTSAC	5 cut(s)	256, 986, 1146, 1608, 1968
TspDTI	ATGAA	5 cut(s)	384, 798, 810, 948, 1434
TspGWI	ACGGA	1 cut(s)	2002
TspRI	CASTG	4 cut(s)	450, 780, 991, 1579
VpaK11BI	GGWCC	3 cut(s)	866, 1535, 1658
XagI	CCTNNNNNAGG	1 cut(s)	314
XapI	RAATTY	1 cut(s)	598
XceI	RCATGY	1 cut(s)	1960
XcmI	CCANNNNNNNNNTGG	3 cut(s)	113, 575, 1091
XmaJI	CCTAGG	1 cut(s)	1334
XspI	CTAG	6 cut(s)	816, 930, 1131, 1335, 1478, 2208
ZrmI	AGTACT	1 cut(s)	1753
Zsp2I	ATGCAT	1 cut(s)	1124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.