Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0007916

Physical Location & Seq

Genomic Coordinates Reverse (-)

6619003 .. 6623550

Gene Structure 4548 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G006430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGGGCTTACCGGAAATCGTCGGTTTTGCTCGGAATTTCGCCGTCATGGTCAGAGTCAAAGGCCCTGACCCCAAAGGCCTCAAAATGCGAAACCATGCTTTCCACCAGTACAACTCTGGGACGACGACTATATCAGCTTCCGGAATGTTATTGCCGGAAACCCTTTACGACGGTGAGGTCGCGAAGCAGCTATGCGGCGGCGGAAGTGACCGGTCACCGGCTCTGGTTGTGACTGTTGCTTCAGTTGTTGAGCCGTTTCTGTCTTTGCAACACAGAGAGAGCCTCGCCCAGGGCAGGCCTGAGTTGATTCCTGGGGTTGAGATTGATGTTATGGCAGAGGATGACATGTTGTTGGAGAAAGGCTCGGAGAAAGGACCTCCATGCTGGTTTGCAGCGCAGCTTTTGACCCTGATTGACGTTCCTGCATCCGCTATTGCACTCCAATCTCTTATTGATGCTTCTATAAGCTCTCCAGAGCATGGATGGGAGGTCGGTTGGTCTTTAGCCTCACATTATAATCCTCAGCCAGTTATGGATGTAAATTTGGCAGTTAGAGAATTGAGGAACGCAAGTCTTACAGGAAAGTCAACTACAAGAATCGCAATTCTTGGAGTATCCTTGCTTTCTAAGGATGTGCCAAATATAACTATATCACCCTCCAATAAAAGGGGAGACTTTCTTATAGCAGTGGGTTCTCCTTTTGGTATCCTGTCACCTGTGCACTTCTTTAACAGCATATCAGTGGGGTCCATTGCCAACTGCTATCCTCCTAATTCATCTTATAGTCCATTGCTGATGGCAGACATACGGTGTCTTCCTGGAGCGGAAGGTGGTCCAGTTTTGAGCGAGAATGCACAACTTATTGGTATGTTGATTAGACCATTGAGGCAGAAGACTAGCGGTGCTGAAGTTCAGCTGGTAATTTCGTGGGAAGCCATTGCAACTGCTTGCAGTGACTTGCTGCAGAAGGAGCCCCATTATGCAGAAAAAGGGATTTATTATGACAAAGGAAACTTAAATGCTGTAGGGAAAGCATTTTTAGCAAACACCAACGGCTCAAATGGACCCATAACGCATATTCAGGAGCATCTTTCTACTAGTTGCTCCCCATCAGCTGTTGAGAAGGCAATAGCTTCTGTTTGTCTTATAACCATAGATGATGGAGTATGGGCATCTGGTGTTTTCCTCAACAAGCAAGGCCTGATACTGACAAATGCTCACCTCTTAGAACCATGGAGATTTGGTAAAAGGACTGCAAGTGACGGATCCATTTCTGAGGCTCCTCCCTTCGTATCTGATGGATCTCTATCTCCAGAGCAAAAGATTCAAGGCTTTCTGCCTGGGCTTCACAAGCATGGAGATCCCTCAGTTGGGAATGAACATGGAGCATTGAACTCATCTTACAAAGGCCATAGAAATATACGTGTCCGCCTGGATCATATGGACCCTTGGATTTGGTGTGATGCCAAAGTAGTCTATGTTTGTAAAGGACCTTTAGATGTTGCCTTGTTACAGCTCCAGTACATCCCAGATCAGCTTTCCCCAGTTGTCATGGACTTTTCATCGCCATCTTTAGGATCAAAGGCATATGTTATTGGACATGGACTGTTTGGACCCAGATGTGGCTTTTCCCCATCAATTTGTTCTGGTGTGGTGGCAAAAGTTGTCAAAGCAAAGTTTCTACTATCATATCAACCCAGTCAACCAGGAAATACTCGAGGGCACTCTCCTGTGATGCTTGAAACAACAGCAGCTGTCCATCCAGGTGGTAGTGGTGGTGCTGTTGTAAATTCAGATGGTCATATGATTGGGCTTGTTACAAGCAACGCTAGACATGGTGGAGGAACAGTTATACCACATCTGAACTTCAGCATTCCATGTGCGGTTCTCCTGCCAATTTTCAAGTTCTCAAAAGACATGCAAGATCTTTCACATCTTCAGGTTCTCGACCAACCAAACAAACATCTTTCTTCTGTATGGGCGTTGATGCCGCATCTGTCTCCAAAGCCCCCTCCTTTGCCTCATATGCAACAGTCATTACCAAATGATCACAACAAAGAAGAGAAAGGTTCTCGCTTTGCAAAATTTTTAGCTGAAAGACGAGATGTGTTTTCAAAGCCAACGCAGCTTAACAGGGCTGGAAGACTATTGAATGACATTGTTCCTAGCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0010467 GO:0016485 GO:0016787 GO:0017171 GO:0019538 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0051604 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Trypsin_2

Protein Analysis

725

Amino Acids

77.79

Weight (kDa)

7.36

Isoelectric Point (pI)

45.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin_2	PF13365	185 - 290	1.1e-08	Trypsin-like peptidase domain
Trypsin_2	PF13365	391 - 605	6e-18	Trypsin-like peptidase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007916)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28320
fragaria_vesca	FvH4_3g08860 FvH4_3g08860 FvH4_3g08860
malus_domestica	MD10G1291600.v1.1
prunus_persica	Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1 Prupe.4G050600_v2.0.a1
pyrus_communis	pycom10g24380
rosa_chinensis	RchiOBHm_Chr5g0008891 RchiOBHm_Chr5g0008901
rosa_laevigata	RLG00000022692 RLG00000031614
rosa_multiflora	Rmu_co8519179.1_g000001 Rmu_sc0004677.1_g000010
rosa_roxburghii	Rroxscaffold_1G00067110 Rroxscaffold_1G00067730
rosa_rugosa	Rorug04G0440000 Rorug04G0440000
rosa_samantha	Rh5AG068700 Rh5BG065400 Rh5CG076600 Rh5DG064400
rosa_wichuraiana	Rw5G006430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	516, 1148
AasI	GACNNNNNNGTC	1 cut(s)	176
AccB7I	CCANNNNNTGG	1 cut(s)	479
AccBSI	CCGCTC	1 cut(s)	824
AccII	CGCG	1 cut(s)	182
AccIII	TCCGGA	1 cut(s)	140
AciI	CCGC	9 cut(s)	195, 198, 201, 429, 824, 900, 1429, 1883, 1991
AclWI	GGATC	6 cut(s)	1260, 1273, 1309, 1355, 1443, 1585
AcsI	RAATTY	4 cut(s)	34, 541, 1789, 2084
AcuI	CTGAAG	4 cut(s)	225, 927, 1852, 1922
AfaI	GTAC	2 cut(s)	110, 1523
AfiI	CCNNNNNNNGG	9 cut(s)	217, 289, 294, 479, 666, 1370, 1371, 1574, 1622
AflIII	ACRYGT	2 cut(s)	345, 1423
AgeI	ACCGGT	1 cut(s)	210
AgsI	TTSAA	6 cut(s)	1328, 1393, 1742, 1903, 2115, 2152
AhlI	ACTAGT	1 cut(s)	1097
AjnI	CCWGG	7 cut(s)	288, 310, 817, 1339, 1431, 1705, 1762
Alw21I	GWGCWC	1 cut(s)	723
Alw26I	GTCTC	2 cut(s)	666, 2004
Alw44I	GTGCAC	1 cut(s)	719
AlwI	GGATC	6 cut(s)	1260, 1273, 1309, 1355, 1443, 1585
AlwNI	CAGNNNCTG	1 cut(s)	1754
Ama87I	CYCGRG	1 cut(s)	1716
Aor13HI	TCCGGA	1 cut(s)	140
AoxI	GGCC	5 cut(s)	61, 76, 296, 1198, 1408
ApaLI	GTGCAC	1 cut(s)	719
ApeKI	GCWGC	6 cut(s)	187, 392, 397, 961, 1751, 2125
ApoI	RAATTY	4 cut(s)	34, 541, 1789, 2084
AsiGI	ACCGGT	1 cut(s)	210
AspLEI	GCGC	1 cut(s)	397
AspS9I	GGNCC	8 cut(s)	62, 374, 747, 833, 1064, 1444, 1490, 1613
AsuHPI	GGTGA	5 cut(s)	185, 207, 645, 705, 1211
AvaI	CYCGRG	1 cut(s)	1716
AvaII	GGWCC	7 cut(s)	374, 747, 833, 1064, 1444, 1490, 1613
BaeGI	GKGCMC	2 cut(s)	723, 1725
BamHI	GGATCC	1 cut(s)	1265
BanII	GRGCYC	1 cut(s)	975
BbsI	GAAGAC	3 cut(s)	806, 899, 2149
Bbv12I	GWGCWC	1 cut(s)	723
BbvCI	CCTCAGC	1 cut(s)	522
BbvI	GCAGC	6 cut(s)	199, 404, 409, 948, 1763, 2137
BccI	CCATC	9 cut(s)	477, 790, 1117, 1154, 1292, 1576, 1642, 1767, 1790
BceAI	ACGGC	3 cut(s)	26, 238, 1069
BciT130I	CCWGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
BciVI	GTATCC	2 cut(s)	625, 716
BclI	TGATCA	1 cut(s)	2047
BcoDI	GTCTC	2 cut(s)	666, 2004
BcuI	ACTAGT	1 cut(s)	1097
BfaI	CTAG	4 cut(s)	897, 1098, 1830, 2166
BfmI	CTRYAG	2 cut(s)	962, 1023
BfuI	GTATCC	2 cut(s)	625, 716
BglII	AGATCT	1 cut(s)	1924
BisI	GCNGC	9 cut(s)	188, 196, 199, 393, 398, 962, 1752, 1991, 2126
BlsI	GCNGC	9 cut(s)	189, 197, 200, 394, 399, 963, 1753, 1992, 2127
Bme1390I	CCNGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
Bme18I	GGWCC	7 cut(s)	374, 747, 833, 1064, 1444, 1490, 1613
BmeT110I	CYCGRG	1 cut(s)	1716
BmgT120I	GGNCC	8 cut(s)	62, 374, 747, 833, 1064, 1444, 1490, 1613
BmiI	GGNNCC	7 cut(s)	748, 972, 1066, 1267, 1281, 1446, 1615
BmrFI	CCNGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
BmrI	ACTGGG	2 cut(s)	1538, 1692
BmsI	GCATC	8 cut(s)	434, 445, 1096, 1181, 1453, 1725, 1977, 2002
BmuI	ACTGGG	2 cut(s)	1538, 1692
BpiI	GAAGAC	3 cut(s)	806, 899, 2149
BplI	GAGNNNNNCTC	4 cut(s)	267, 299, 347, 379
BpmI	CTGGAG	4 cut(s)	456, 840, 1296, 1502
Bpu10I	CCTNAGC	1 cut(s)	522
BsaAI	YACGTR	1 cut(s)	1424
BsaBI	GATNNNNATC	1 cut(s)	1306
BsaJI	CCNNGG	6 cut(s)	288, 289, 311, 1232, 1340, 1448
BsaWI	WCCGGW	3 cut(s)	10, 140, 210
BsaXI	ACNNNNNCTCC	4 cut(s)	1228, 1258, 1833, 1863
Bsc4I	CCNNNNNNNGG	9 cut(s)	217, 289, 294, 479, 666, 1370, 1371, 1574, 1622
Bse118I	RCCGGY	2 cut(s)	210, 217
Bse1I	ACTGG	6 cut(s)	106, 527, 836, 1519, 1544, 1698
Bse3DI	GCAATG	3 cut(s)	750, 788, 936
Bse8I	GATNNNNATC	1 cut(s)	1306
BseAI	TCCGGA	1 cut(s)	140
BseBI	CCWGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
BseDI	CCNNGG	6 cut(s)	288, 289, 311, 1232, 1340, 1448
BseGI	GGATG	7 cut(s)	346, 425, 488, 541, 637, 1524, 1759
BseJI	GATNNNNATC	1 cut(s)	1306
BseLI	CCNNNNNNNGG	9 cut(s)	217, 289, 294, 479, 666, 1370, 1371, 1574, 1622
BseMI	GCAATG	3 cut(s)	750, 788, 936
BseMII	CTCAG	4 cut(s)	291, 536, 1266, 1380
BseNI	ACTGG	6 cut(s)	106, 527, 836, 1519, 1544, 1698
BseRI	GAGGAG	1 cut(s)	1272
BseSI	GKGCMC	2 cut(s)	723, 1725
BseXI	GCAGC	6 cut(s)	199, 404, 409, 948, 1763, 2137
Bsh1236I	CGCG	1 cut(s)	182
BshFI	GGCC	5 cut(s)	63, 78, 298, 1200, 1410
BshTI	ACCGGT	1 cut(s)	210
BsiHKAI	GWGCWC	1 cut(s)	723
BsiHKCI	CYCGRG	1 cut(s)	1716
BsiSI	CCGG	5 cut(s)	11, 141, 155, 211, 218
BslFI	GGGAC	1 cut(s)	133
BslI	CCNNNNNNNGG	9 cut(s)	217, 289, 294, 479, 666, 1370, 1371, 1574, 1622
BsmAI	GTCTC	2 cut(s)	666, 2004
BsmFI	GGGAC	1 cut(s)	133
BsmI	GAATGC	2 cut(s)	856, 1872
BsnI	GGCC	5 cut(s)	63, 78, 298, 1200, 1410
BsoBI	CYCGRG	1 cut(s)	1716
Bsp1286I	GDGCHC	3 cut(s)	723, 975, 1725
Bsp13I	TCCGGA	1 cut(s)	140
Bsp143I	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
Bsp19I	CCATGG	1 cut(s)	1232
Bsp68I	TCGCGA	1 cut(s)	182
BspACI	CCGC	9 cut(s)	195, 198, 201, 429, 824, 900, 1429, 1883, 1991
BspANI	GGCC	5 cut(s)	63, 78, 298, 1200, 1410
BspCNI	CTCAG	4 cut(s)	292, 535, 1267, 1379
BspEI	TCCGGA	1 cut(s)	140
BspFNI	CGCG	1 cut(s)	182
BspLI	GGNNCC	7 cut(s)	748, 972, 1066, 1267, 1281, 1446, 1615
BspMAI	CTGCAG	1 cut(s)	966
BspPI	GGATC	6 cut(s)	1260, 1273, 1309, 1355, 1443, 1585
BsrBI	CCGCTC	1 cut(s)	824
BsrDI	GCAATG	3 cut(s)	750, 788, 936
BsrFI	RCCGGY	2 cut(s)	210, 217
BsrI	ACTGG	6 cut(s)	106, 527, 836, 1519, 1544, 1698
BssAI	RCCGGY	2 cut(s)	210, 217
BssECI	CCNNGG	6 cut(s)	288, 289, 311, 1232, 1340, 1448
BssMI	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
BssT1I	CCWWGG	2 cut(s)	1232, 1448
Bst2UI	CCWGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
Bst4CI	ACNGT	6 cut(s)	173, 235, 810, 1608, 1849, 2034
Bst6I	CTCTTC	1 cut(s)	2055
BstBAI	YACGTR	1 cut(s)	1424
BstC8I	GCNNGC	2 cut(s)	296, 949
BstDEI	CTNAG	6 cut(s)	300, 522, 627, 1225, 1275, 1366
BstDSI	CCRYGG	1 cut(s)	1232
BstEII	GGTNACC	1 cut(s)	213
BstENI	CCTNNNNNAGG	1 cut(s)	287
BstF5I	GGATG	7 cut(s)	346, 425, 488, 541, 637, 1524, 1759
BstFNI	CGCG	1 cut(s)	182
BstHHI	GCGC	1 cut(s)	397
BstKTI	GATC	8 cut(s)	1268, 1304, 1363, 1438, 1534, 1580, 1927, 2050
BstMAI	GTCTC	2 cut(s)	666, 2004
BstMBI	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
BstMWI	GCNNNNNNNGC	5 cut(s)	970, 1040, 1351, 2026, 2125
BstNI	CCWGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
BstNSI	RCATGY	2 cut(s)	349, 1921
BstPI	GGTNACC	1 cut(s)	213
BstSCI	CCNGG	7 cut(s)	288, 310, 817, 1339, 1431, 1705, 1762
BstSFI	CTRYAG	2 cut(s)	962, 1023
BstSLI	GKGCMC	2 cut(s)	723, 1725
BstUI	CGCG	1 cut(s)	182
BstV1I	GCAGC	6 cut(s)	199, 404, 409, 948, 1763, 2137
BstV2I	GAAGAC	3 cut(s)	806, 899, 2149
BstX2I	RGATCY	4 cut(s)	1265, 1301, 1360, 1924
BstXI	CCANNNNNNTGG	1 cut(s)	1766
BstYI	RGATCY	4 cut(s)	1265, 1301, 1360, 1924
BsuI	GTATCC	2 cut(s)	625, 716
BsuRI	GGCC	5 cut(s)	63, 78, 298, 1200, 1410
BtgI	CCRYGG	1 cut(s)	1232
BtgZI	GCGATG	1 cut(s)	1548
BtsCI	GGATG	7 cut(s)	346, 425, 488, 541, 637, 1524, 1759
BtsI	GCAGTG	2 cut(s)	693, 958
BtsIMutI	CAGTG	3 cut(s)	693, 747, 958
BtuMI	TCGCGA	1 cut(s)	182
Cac8I	GCNNGC	2 cut(s)	296, 949
CaiI	CAGNNNCTG	1 cut(s)	1754
CfoI	GCGC	1 cut(s)	397
Cfr10I	RCCGGY	2 cut(s)	210, 217
Cfr13I	GGNCC	8 cut(s)	62, 374, 747, 833, 1064, 1444, 1490, 1613
Csp6I	GTAC	2 cut(s)	109, 1522
CspAI	ACCGGT	1 cut(s)	210
CviQI	GTAC	2 cut(s)	109, 1522
DdeI	CTNAG	6 cut(s)	300, 522, 627, 1225, 1275, 1366
DpnI	GATC	8 cut(s)	1267, 1303, 1362, 1437, 1533, 1579, 1926, 2049
DpnII	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
DrdI	GACNNNNNNGTC	1 cut(s)	176
DseDI	GACNNNNNNGTC	1 cut(s)	176
Eam1104I	CTCTTC	1 cut(s)	2055
EarI	CTCTTC	1 cut(s)	2055
EciI	GGCGGA	2 cut(s)	216, 1418
Eco130I	CCWWGG	2 cut(s)	1232, 1448
Eco147I	AGGCCT	3 cut(s)	78, 298, 1200
Eco24I	GRGCYC	1 cut(s)	975
Eco47I	GGWCC	7 cut(s)	374, 747, 833, 1064, 1444, 1490, 1613
Eco57I	CTGAAG	4 cut(s)	225, 927, 1852, 1922
Eco88I	CYCGRG	1 cut(s)	1716
Eco91I	GGTNACC	1 cut(s)	213
EcoNI	CCTNNNNNAGG	1 cut(s)	287
EcoO109I	RGGNCCY	3 cut(s)	62, 374, 1490
EcoO65I	GGTNACC	1 cut(s)	213
EcoRII	CCWGG	7 cut(s)	288, 310, 817, 1339, 1431, 1705, 1762
EcoT14I	CCWWGG	2 cut(s)	1232, 1448
EcoT38I	GRGCYC	1 cut(s)	975
ErhI	CCWWGG	2 cut(s)	1232, 1448
FaqI	GGGAC	1 cut(s)	133
FauNDI	CATATG	4 cut(s)	1440, 1588, 1803, 2025
FbaI	TGATCA	1 cut(s)	2047
Fnu4HI	GCNGC	9 cut(s)	188, 196, 199, 393, 398, 962, 1752, 1991, 2126
FokI	GGATG	7 cut(s)	353, 412, 495, 548, 644, 1511, 1746
FriOI	GRGCYC	1 cut(s)	975
Fsp4HI	GCNGC	9 cut(s)	188, 196, 199, 393, 398, 962, 1752, 1991, 2126
FspBI	CTAG	4 cut(s)	897, 1098, 1830, 2166
GlaI	GCGC	1 cut(s)	396
GluI	GCNGC	9 cut(s)	188, 196, 199, 393, 398, 962, 1752, 1991, 2126
GsuI	CTGGAG	4 cut(s)	456, 840, 1296, 1502
HaeIII	GGCC	5 cut(s)	63, 78, 298, 1200, 1410
HapII	CCGG	5 cut(s)	11, 141, 155, 211, 218
HhaI	GCGC	1 cut(s)	397
Hin6I	GCGC	1 cut(s)	395
HinP1I	GCGC	1 cut(s)	395
HincII	GTYRAC	2 cut(s)	588, 1703
HindII	GTYRAC	2 cut(s)	588, 1703
HinfI	GANTC	4 cut(s)	54, 307, 597, 1324
HpaII	CCGG	5 cut(s)	11, 141, 155, 211, 218
HphI	GGTGA	5 cut(s)	185, 207, 645, 705, 1211
Hpy166II	GTNNAC	3 cut(s)	588, 721, 1703
Hpy188I	TCNGA	7 cut(s)	33, 53, 367, 1276, 1297, 1795, 1863
Hpy188III	TCNNGA	6 cut(s)	141, 181, 473, 1082, 1313, 1946
Hpy8I	GTNNAC	3 cut(s)	588, 721, 1703
Hpy99I	CGWCG	3 cut(s)	23, 127, 173
HpyAV	CCTTC	4 cut(s)	821, 961, 1117, 1297
HpyCH4III	ACNGT	6 cut(s)	173, 235, 810, 1608, 1849, 2034
HpyCH4IV	ACGT	2 cut(s)	417, 1423
HpyF10VI	GCNNNNNNNGC	5 cut(s)	970, 1040, 1351, 2026, 2125
HpyF3I	CTNAG	6 cut(s)	300, 522, 627, 1225, 1275, 1366
HpySE526I	ACGT	2 cut(s)	417, 1423
HspAI	GCGC	1 cut(s)	395
Kpn2I	TCCGGA	1 cut(s)	140
Ksp22I	TGATCA	1 cut(s)	2047
Kzo9I	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
LmnI	GCTCC	7 cut(s)	821, 970, 1084, 1109, 1285, 1385, 1521
Lsp1109I	GCAGC	6 cut(s)	199, 404, 409, 948, 1763, 2137
LweI	GCATC	8 cut(s)	434, 445, 1096, 1181, 1453, 1725, 1977, 2002
MaeI	CTAG	4 cut(s)	897, 1098, 1830, 2166
MaeII	ACGT	2 cut(s)	417, 1423
MaeIII	GTNAC	8 cut(s)	206, 213, 229, 711, 953, 1259, 1509, 1816
MalI	GATC	8 cut(s)	1267, 1303, 1362, 1437, 1533, 1579, 1926, 2049
MbiI	CCGCTC	1 cut(s)	824
MboI	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
MboII	GAAGA	6 cut(s)	806, 904, 1928, 1962, 2072, 2154
MflI	RGATCY	4 cut(s)	1265, 1301, 1360, 1924
MhlI	GDGCHC	3 cut(s)	723, 975, 1725
MlyI	GAGTC	1 cut(s)	63
MmeI	TCCRAC	1 cut(s)	333
MroI	TCCGGA	1 cut(s)	140
MseI	TTAA	3 cut(s)	729, 1016, 2130
MslI	CAYNNNNRTG	3 cut(s)	740, 1353, 1764
MspA1I	CMGCKG	3 cut(s)	916, 1115, 1754
MspI	CCGG	5 cut(s)	11, 141, 155, 211, 218
MspR9I	CCNGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
Mva1269I	GAATGC	2 cut(s)	856, 1872
MvaI	CCWGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
MvnI	CGCG	1 cut(s)	182
MwoI	GCNNNNNNNGC	5 cut(s)	970, 1040, 1351, 2026, 2125
NcoI	CCATGG	1 cut(s)	1232
NdeI	CATATG	4 cut(s)	1440, 1588, 1803, 2025
NdeII	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
NlaIV	GGNNCC	7 cut(s)	748, 972, 1066, 1267, 1281, 1446, 1615
NmuCI	GTSAC	6 cut(s)	206, 213, 229, 711, 953, 1259
NruI	TCGCGA	1 cut(s)	182
NspI	RCATGY	2 cut(s)	349, 1921
PaeR7I	CTCGAG	1 cut(s)	1716
PasI	CCCWGGG	1 cut(s)	289
PceI	AGGCCT	3 cut(s)	78, 298, 1200
PciI	ACATGT	1 cut(s)	345
PctI	GAATGC	2 cut(s)	856, 1872
PfeI	GAWTC	3 cut(s)	307, 597, 1324
PflMI	CCANNNNNTGG	1 cut(s)	479
PfoI	TCCNGGA	1 cut(s)	817
PinAI	ACCGGT	1 cut(s)	210
PkrI	GCNGC	9 cut(s)	189, 197, 200, 394, 399, 963, 1753, 1992, 2127
PleI	GAGTC	1 cut(s)	62
PpsI	GAGTC	1 cut(s)	62
Ppu21I	YACGTR	1 cut(s)	1424
PpuMI	RGGWCCY	2 cut(s)	374, 1490
PscI	ACATGT	1 cut(s)	345
PsiI	TTATAA	2 cut(s)	516, 1148
Psp5II	RGGWCCY	2 cut(s)	374, 1490
Psp6I	CCWGG	7 cut(s)	288, 310, 817, 1339, 1431, 1705, 1762
PspEI	GGTNACC	1 cut(s)	213
PspGI	CCWGG	7 cut(s)	288, 310, 817, 1339, 1431, 1705, 1762
PspN4I	GGNNCC	7 cut(s)	748, 972, 1066, 1267, 1281, 1446, 1615
PspPI	GGNCC	8 cut(s)	62, 374, 747, 833, 1064, 1444, 1490, 1613
PspPPI	RGGWCCY	2 cut(s)	374, 1490
PspXI	VCTCGAGB	1 cut(s)	1716
PsrI	GAACNNNNNNTAC	2 cut(s)	1385, 1417
PstI	CTGCAG	1 cut(s)	966
PstNI	CAGNNNCTG	1 cut(s)	1754
PsuI	RGATCY	4 cut(s)	1265, 1301, 1360, 1924
PvuII	CAGCTG	3 cut(s)	916, 1115, 1754
RruI	TCGCGA	1 cut(s)	182
RsaI	GTAC	2 cut(s)	110, 1523
RsaNI	GTAC	2 cut(s)	109, 1522
RseI	CAYNNNNRTG	3 cut(s)	740, 1353, 1764
SaqAI	TTAA	3 cut(s)	729, 1016, 2130
SatI	GCNGC	9 cut(s)	188, 196, 199, 393, 398, 962, 1752, 1991, 2126
Sau3AI	GATC	8 cut(s)	1265, 1301, 1360, 1435, 1531, 1577, 1924, 2047
Sau96I	GGNCC	8 cut(s)	62, 374, 747, 833, 1064, 1444, 1490, 1613
SchI	GAGTC	1 cut(s)	63
ScrFI	CCNGG	7 cut(s)	290, 312, 819, 1341, 1433, 1707, 1764
SduI	GDGCHC	3 cut(s)	723, 975, 1725
SfaNI	GCATC	8 cut(s)	434, 445, 1096, 1181, 1453, 1725, 1977, 2002
SfcI	CTRYAG	2 cut(s)	962, 1023
Sfr274I	CTCGAG	1 cut(s)	1716
SinI	GGWCC	7 cut(s)	374, 747, 833, 1064, 1444, 1490, 1613
SlaI	CTCGAG	1 cut(s)	1716
SmiMI	CAYNNNNRTG	3 cut(s)	740, 1353, 1764
SmlI	CTYRAG	1 cut(s)	1716
SmoI	CTYRAG	1 cut(s)	1716
SpeI	ACTAGT	1 cut(s)	1097
SseBI	AGGCCT	3 cut(s)	78, 298, 1200
SsiI	CCGC	9 cut(s)	195, 198, 201, 429, 824, 900, 1429, 1883, 1991
SspMI	CTAG	4 cut(s)	897, 1098, 1830, 2166
StuI	AGGCCT	3 cut(s)	78, 298, 1200
StyD4I	CCNGG	7 cut(s)	288, 310, 817, 1339, 1431, 1705, 1762
StyI	CCWWGG	2 cut(s)	1232, 1448
TaaI	ACNGT	6 cut(s)	173, 235, 810, 1608, 1849, 2034
TaiI	ACGT	2 cut(s)	420, 1426
TaqI	TCGA	2 cut(s)	1717, 1947
TatI	WGTACW	2 cut(s)	108, 1521
TauI	GCSGC	3 cut(s)	198, 201, 1993
TfiI	GAWTC	3 cut(s)	307, 597, 1324
Tru1I	TTAA	3 cut(s)	729, 1016, 2130
Tru9I	TTAA	3 cut(s)	729, 1016, 2130
TscAI	CASTG	3 cut(s)	693, 747, 958
TseFI	GTSAC	6 cut(s)	206, 213, 229, 711, 953, 1259
TseI	GCWGC	6 cut(s)	187, 392, 397, 961, 1751, 2125
Tsp45I	GTSAC	6 cut(s)	206, 213, 229, 711, 953, 1259
TspDTI	ATGAA	3 cut(s)	765, 1392, 1551
TspGWI	ACGGA	1 cut(s)	1278
TspRI	CASTG	3 cut(s)	693, 747, 958
Van91I	CCANNNNNTGG	1 cut(s)	479
VneI	GTGCAC	1 cut(s)	719
VpaK11BI	GGWCC	7 cut(s)	374, 747, 833, 1064, 1444, 1490, 1613
XagI	CCTNNNNNAGG	1 cut(s)	287
XapI	RAATTY	4 cut(s)	34, 541, 1789, 2084
XceI	RCATGY	2 cut(s)	349, 1921
XcmI	CCANNNNNNNNNTGG	2 cut(s)	113, 480
XhoI	CTCGAG	1 cut(s)	1716
XspI	CTAG	4 cut(s)	897, 1098, 1830, 2166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.