RchiOBHm_Chr5g0008891

Glyoxysomal processing protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007916
Physical Location & Seq
Reverse (-)
5835580 .. 5837576
1997 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28982

Sequence Viewer

Length: 1107 bp
ATGCATATTCAGGAGCATCTTTCTACTAGTTGCTCCCCATCAGCTGTTGAGAAGGCAATAGCTTCTGTTTGTCTTATAACCATAGATGATGGAGTATGGGCATCTGGTGTTTTCCTCAACAAGCAAGGCCTGATACTGACAAATGCTCACCTCTTAGAACCATGGAGATTTGGTAAAAGGACTGCAAGTGACGGATCCATTTCTGAGGCTTCTCCCCTCCTATCTGATGGATCTCTATCTCCAGAGCAAAAGATTCAAGGCTTTCTGCCTGGGCTTCACAAGCATGGAGATCCCTCAGTTGGGAATGAACATGGAGCATTGAACTCATCTTACAAAGGCCATAGAAATATACGTGTCCGCCTGGATCATATGGACCCTTGGATTTGGTGTGACGCCAAAGTAGTCTATGTTTGTAAAGGACCTTTAGATGTTGCCTTGTTACAGCTCCAGTACATCCCAGATCAGCTTTCCCCAGTTGTCATGGACTTTTCATCGCCATCTTTAGGATCAAAGGCATATGTTATTGGACATGGACTGTTTGGACCCAGATGTGGCTTTTCCCCATCAATTTGTTCTGGTGTGGTGGCAAAAGTTGTCAAAGCAAAGTTTCAACTATCACATCAACCCAGTCAACCAGGAAATACTCGAGGGCACTCTCCTGTGATGCTTGAAACAACTGCAGCTGTCCATCCAGGTGGTAGTGGTGGTGCTGTTGTAAATTCAGATGGTCATATGATTGGGCTTGTTACAAGCAACGCTAGACATGGTGGAGGAACAGTTATACCACATCTGAACTTCAGCATTCCATGTGCGGCTCTCCTGCCAATTTTCAAGTTCTCAAAAGACATGCAAGATCTTTCACATCTTCAGGTTCTCGACCAACCAAACAAACATCTTTCTTCTGTGTGGGCGTTGATGCCGCATCTGTCTCCAAAGCCCCCTCCTTTGCCTCATATGCAACAGTCATTACCAAATGATCACAACAAAGAAGAGAAAGGTTCTCGCTTTGCAAAATTTTTAGCTGAAAGACGAGATGTGTTTTCAAAGCCAACACAGCTTAACAGGGCTGGAAGACTATTGAACGACATTGTTCCTAGCAAGTTATGA

Protein Analysis

368

Amino Acids

39.76

Weight (kDa)

8.91

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin_2 PF13365 34 - 248 1.2e-18 Trypsin-like peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 77
AciI CCGC 3 cut(s) 358, 812, 920
AclWI GGATC 6 cut(s) 189, 202, 238, 284, 372, 514
AcsI RAATTY 2 cut(s) 718, 1013
AcuI CTGAAG 2 cut(s) 781, 851
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 4 cut(s) 299, 300, 503, 551
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 7 cut(s) 257, 322, 611, 671, 832, 1044, 1081
AhlI ACTAGT 1 cut(s) 26
AjnI CCWGG 4 cut(s) 268, 360, 634, 691
AluBI AGCT 7 cut(s) 44, 62, 445, 466, 683, 1022, 1057
AluI AGCT 7 cut(s) 44, 62, 445, 466, 683, 1022, 1057
Alw26I GTCTC 1 cut(s) 933
AlwI GGATC 6 cut(s) 189, 202, 238, 284, 372, 514
Ama87I CYCGRG 1 cut(s) 645
AoxI GGCC 2 cut(s) 127, 337
ApeKI GCWGC 1 cut(s) 680
ApoI RAATTY 2 cut(s) 718, 1013
AspS9I GGNCC 3 cut(s) 373, 419, 542
AsuHPI GGTGA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 645
AvaII GGWCC 3 cut(s) 373, 419, 542
BaeGI GKGCMC 1 cut(s) 654
BamHI GGATCC 1 cut(s) 194
BbsI GAAGAC 1 cut(s) 1078
BbvI GCAGC 1 cut(s) 692
BccI CCATC 7 cut(s) 46, 83, 221, 505, 571, 696, 719
BciT130I CCWGG 4 cut(s) 270, 362, 636, 693
BclI TGATCA 1 cut(s) 976
BcoDI GTCTC 1 cut(s) 933
BcuI ACTAGT 1 cut(s) 26
BfaI CTAG 3 cut(s) 27, 759, 1095
BfmI CTRYAG 1 cut(s) 678
BglII AGATCT 1 cut(s) 853
BisI GCNGC 3 cut(s) 681, 813, 920
BlsI GCNGC 3 cut(s) 682, 814, 921
Bme1390I CCNGG 4 cut(s) 270, 362, 636, 693
Bme18I GGWCC 3 cut(s) 373, 419, 542
BmeT110I CYCGRG 1 cut(s) 645
BmgT120I GGNCC 3 cut(s) 373, 419, 542
BmiI GGNNCC 3 cut(s) 196, 375, 544
BmrFI CCNGG 4 cut(s) 270, 362, 636, 693
BmrI ACTGGG 2 cut(s) 467, 621
BmsI GCATC 5 cut(s) 25, 110, 654, 906, 931
BmuI ACTGGG 2 cut(s) 467, 621
BpiI GAAGAC 1 cut(s) 1078
BpmI CTGGAG 2 cut(s) 225, 431
BsaAI YACGTR 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 235
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 3 cut(s) 161, 269, 377
BsaXI ACNNNNNCTCC 4 cut(s) 157, 187, 762, 792
Bsc4I CCNNNNNNNGG 4 cut(s) 299, 300, 503, 551
Bse1I ACTGG 3 cut(s) 448, 473, 627
Bse8I GATNNNNATC 1 cut(s) 235
BseBI CCWGG 4 cut(s) 270, 362, 636, 693
BseDI CCNNGG 3 cut(s) 161, 269, 377
BseGI GGATG 2 cut(s) 453, 688
BseJI GATNNNNATC 1 cut(s) 235
BseLI CCNNNNNNNGG 4 cut(s) 299, 300, 503, 551
BseMII CTCAG 2 cut(s) 195, 309
BseNI ACTGG 3 cut(s) 448, 473, 627
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 1 cut(s) 692
BshFI GGCC 2 cut(s) 129, 339
BsiHKCI CYCGRG 1 cut(s) 645
BslI CCNNNNNNNGG 4 cut(s) 299, 300, 503, 551
BsmAI GTCTC 1 cut(s) 933
BsmI GAATGC 1 cut(s) 801
BsnI GGCC 2 cut(s) 129, 339
BsoBI CYCGRG 1 cut(s) 645
Bsp1286I GDGCHC 1 cut(s) 654
Bsp143I GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 3 cut(s) 358, 812, 920
BspANI GGCC 2 cut(s) 129, 339
BspCNI CTCAG 2 cut(s) 196, 308
BspLI GGNNCC 3 cut(s) 196, 375, 544
BspMAI CTGCAG 1 cut(s) 682
BspPI GGATC 6 cut(s) 189, 202, 238, 284, 372, 514
BsrI ACTGG 3 cut(s) 448, 473, 627
BssECI CCNNGG 3 cut(s) 161, 269, 377
BssMI GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 2 cut(s) 161, 377
Bst2UI CCWGG 4 cut(s) 270, 362, 636, 693
Bst4CI ACNGT 3 cut(s) 537, 778, 963
Bst6I CTCTTC 1 cut(s) 984
BstACI GRCGYC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 353
BstDEI CTNAG 3 cut(s) 154, 204, 295
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 2 cut(s) 453, 688
BstKTI GATC 8 cut(s) 197, 233, 292, 367, 463, 509, 856, 979
BstMAI GTCTC 1 cut(s) 933
BstMBI GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
BstMWI GCNNNNNNNGC 3 cut(s) 280, 955, 1054
BstNI CCWGG 4 cut(s) 270, 362, 636, 693
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 4 cut(s) 268, 360, 634, 691
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 692
BstV2I GAAGAC 1 cut(s) 1078
BstX2I RGATCY 4 cut(s) 194, 230, 289, 853
BstXI CCANNNNNNTGG 1 cut(s) 695
BstYI RGATCY 4 cut(s) 194, 230, 289, 853
BsuRI GGCC 2 cut(s) 129, 339
BtgI CCRYGG 1 cut(s) 161
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 453, 688
Cfr13I GGNCC 3 cut(s) 373, 419, 542
CseI GACGC 1 cut(s) 401
Csp6I GTAC 1 cut(s) 451
CviAII CATG 8 cut(s) 162, 284, 311, 481, 530, 764, 807, 847
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 3 cut(s) 154, 204, 295
DpnI GATC 8 cut(s) 196, 232, 291, 366, 462, 508, 855, 978
DpnII GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
Eam1104I CTCTTC 1 cut(s) 984
EarI CTCTTC 1 cut(s) 984
EciI GGCGGA 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 161, 377
Eco147I AGGCCT 1 cut(s) 129
Eco47I GGWCC 3 cut(s) 373, 419, 542
Eco57I CTGAAG 2 cut(s) 781, 851
Eco88I CYCGRG 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 419
EcoRII CCWGG 4 cut(s) 268, 360, 634, 691
EcoT14I CCWWGG 2 cut(s) 161, 377
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 161, 377
FaeI CATG 8 cut(s) 165, 287, 314, 484, 533, 767, 810, 850
FatI CATG 8 cut(s) 161, 283, 310, 480, 529, 763, 806, 846
FauNDI CATATG 4 cut(s) 369, 517, 732, 954
FbaI TGATCA 1 cut(s) 976
Fnu4HI GCNGC 3 cut(s) 681, 813, 920
FokI GGATG 2 cut(s) 440, 675
Fsp4HI GCNGC 3 cut(s) 681, 813, 920
FspBI CTAG 3 cut(s) 27, 759, 1095
GluI GCNGC 3 cut(s) 681, 813, 920
GsuI CTGGAG 2 cut(s) 225, 431
HaeIII GGCC 2 cut(s) 129, 339
HgaI GACGC 1 cut(s) 401
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 8 cut(s) 165, 287, 314, 484, 533, 767, 810, 850
HincII GTYRAC 1 cut(s) 632
HindII GTYRAC 1 cut(s) 632
HinfI GANTC 1 cut(s) 253
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 1 cut(s) 632
Hpy188I TCNGA 4 cut(s) 205, 226, 724, 792
Hpy188III TCNNGA 3 cut(s) 11, 242, 875
Hpy8I GTNNAC 1 cut(s) 632
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 3 cut(s) 537, 778, 963
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 6 cut(s) 4, 185, 680, 850, 958, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 955, 1054
HpyF3I CTNAG 3 cut(s) 154, 204, 295
HpySE526I ACGT 1 cut(s) 352
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 8 cut(s) 165, 287, 314, 484, 533, 767, 810, 850
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
LmnI GCTCC 4 cut(s) 13, 38, 314, 450
Lsp1109I GCAGC 1 cut(s) 692
LweI GCATC 5 cut(s) 25, 110, 654, 906, 931
MaeI CTAG 3 cut(s) 27, 759, 1095
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 4 cut(s) 188, 389, 438, 745
MalI GATC 8 cut(s) 196, 232, 291, 366, 462, 508, 855, 978
MboI GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
MboII GAAGA 4 cut(s) 857, 891, 1001, 1083
MflI RGATCY 4 cut(s) 194, 230, 289, 853
MhlI GDGCHC 1 cut(s) 654
MluCI AATT 4 cut(s) 567, 718, 825, 1013
MnlI CCTC 9 cut(s) 125, 161, 199, 227, 304, 641, 764, 951, 960
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 1059
MslI CAYNNNNRTG 2 cut(s) 282, 693
MspA1I CMGCKG 2 cut(s) 44, 683
MspR9I CCNGG 4 cut(s) 270, 362, 636, 693
Mva1269I GAATGC 1 cut(s) 801
MvaI CCWGG 4 cut(s) 270, 362, 636, 693
MwoI GCNNNNNNNGC 3 cut(s) 280, 955, 1054
NcoI CCATGG 1 cut(s) 161
NdeI CATATG 4 cut(s) 369, 517, 732, 954
NdeII GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
NlaIII CATG 8 cut(s) 165, 287, 314, 484, 533, 767, 810, 850
NlaIV GGNNCC 3 cut(s) 196, 375, 544
NmuCI GTSAC 2 cut(s) 188, 389
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 850
PaeR7I CTCGAG 1 cut(s) 645
PceI AGGCCT 1 cut(s) 129
PctI GAATGC 1 cut(s) 801
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 3 cut(s) 682, 814, 921
Ppu21I YACGTR 1 cut(s) 353
PpuMI RGGWCCY 1 cut(s) 419
PsiI TTATAA 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 4 cut(s) 268, 360, 634, 691
PspGI CCWGG 4 cut(s) 268, 360, 634, 691
PspN4I GGNNCC 3 cut(s) 196, 375, 544
PspPI GGNCC 3 cut(s) 373, 419, 542
PspPPI RGGWCCY 1 cut(s) 419
PspXI VCTCGAGB 1 cut(s) 645
PsrI GAACNNNNNNTAC 2 cut(s) 314, 346
PstI CTGCAG 1 cut(s) 682
PsuI RGATCY 4 cut(s) 194, 230, 289, 853
PvuII CAGCTG 2 cut(s) 44, 683
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 2 cut(s) 282, 693
SaqAI TTAA 1 cut(s) 1059
SatI GCNGC 3 cut(s) 681, 813, 920
Sau3AI GATC 8 cut(s) 194, 230, 289, 364, 460, 506, 853, 976
Sau96I GGNCC 3 cut(s) 373, 419, 542
ScrFI CCNGG 4 cut(s) 270, 362, 636, 693
SduI GDGCHC 1 cut(s) 654
SfaNI GCATC 5 cut(s) 25, 110, 654, 906, 931
SfcI CTRYAG 1 cut(s) 678
Sfr274I CTCGAG 1 cut(s) 645
SinI GGWCC 3 cut(s) 373, 419, 542
SlaI CTCGAG 1 cut(s) 645
SmiMI CAYNNNNRTG 2 cut(s) 282, 693
SmlI CTYRAG 1 cut(s) 645
SmoI CTYRAG 1 cut(s) 645
SpeI ACTAGT 1 cut(s) 26
Sse9I AATT 4 cut(s) 567, 718, 825, 1013
SseBI AGGCCT 1 cut(s) 129
SsiI CCGC 3 cut(s) 358, 812, 920
SspMI CTAG 3 cut(s) 27, 759, 1095
StuI AGGCCT 1 cut(s) 129
StyD4I CCNGG 4 cut(s) 268, 360, 634, 691
StyI CCWWGG 2 cut(s) 161, 377
TaaI ACNGT 3 cut(s) 537, 778, 963
TaiI ACGT 1 cut(s) 355
TaqI TCGA 2 cut(s) 646, 876
TasI AATT 4 cut(s) 567, 718, 825, 1013
TatI WGTACW 1 cut(s) 450
TauI GCSGC 2 cut(s) 815, 922
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TseFI GTSAC 2 cut(s) 188, 389
TseI GCWGC 1 cut(s) 680
Tsp45I GTSAC 2 cut(s) 188, 389
TspDTI ATGAA 2 cut(s) 321, 480
TspGWI ACGGA 1 cut(s) 207
VpaK11BI GGWCC 3 cut(s) 373, 419, 542
XapI RAATTY 2 cut(s) 718, 1013
XceI RCATGY 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 645
XspI CTAG 3 cut(s) 27, 759, 1095
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.