AT1G30840

Purine nucleobase transmembrane transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015201
Physical Location & Seq
Forward (+)
10974383 .. 10976298
1916 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30840.1

Sequence Viewer

Length: 1149 bp
ATGAGTGATGGTCGAGTTAACGCAGATCCTCAACAAGAAGAGAACATGGTTAAACCACCGGTTAAGAGATCTCTCACTCTCCTCATCGTGACTTACTTTTTTCTCTTCTTCGGCTCTATCGCTTCGAGCCTTCTCGCGAAGTACTATTTCGTCTACGGTGGCTCGAGCCGGTGGGTCTCCACGTGGGTTCAATCTGCTGGCTTCCCTCTCCTCCTCATCCTAATCTACTTCCCTCACTACGTTCTCAAAACCACCACTCGCCGTCCTTTCACGCGCTTCACGCTCCGCCATCTCATCTTCTCCGTGTTGATCGGGCTTGTTCTCGGTTTCAACAACTTCCTCTTCTCATGGGGAACCTCGTACCTTCCGGTGTCCACGTCATCGCTTCTCCTCTCGACACAACTCGTCTTCACTCTCATTTTGTCTAGGATCATAGTGAAACAGAAGATCACTTTCTCAAACCTCAACTGCGTTGTCCTGTTGACGTTAAGCTCTGTTTTGTTGGCTCTTGATTCGAGTAAAGATAAACCGTCCGGTTTAACCAAAACCAAGTATTTCATCGGGTATGTATCCACGATCGGAGCCGGTTTACTCTTCGCTCTTTACCTCCCCGTGACGGAGAAGCTATACCGTACCGTTTATTGTTACGCGATGGTCATGGAGGTGCAACTGGTGATGGAATTCGCAGCGACGGTTTTCGCGACAATCGGTATGGCTTGCGAAGGCGGGTTTAAGGAAATGGTTAAGGAAGCGAACCATGTTTTCACCAAAGGACCAACATTTTATTGGACGTTTGCGATACTAGCAAATGTGGTGACGTGGCAGCTCTCTTTCGCAGCCACGTCAGGGATGGTTTACTTGACGTCAGGTATCACCGGAGGTATCTGCATGACGGCGTTGCTCGCGATGAATGTGATAGGAGGTGTGGTGGCTTACGGTGACGTGTTTGGTGGAGTGAAGATTGTGTCGACGGTGCTATGTATTTGGGGATTCTCATCTTACACATACGGGATGTATATGAAGATGAAGAAGGAGGAGAAGGAGAAGGGAGAATATTCCGGCGTAAAGACGACGGAAGACAGCGGAGAGATGGAGGTGGAAATGGGAAATGTTAAAGATGACGTGGCGGCGGCGGATGATAGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

382

Amino Acids

42.32

Weight (kDa)

8.87

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 27 - 344 8.4e-107 Purine nucleobase transmembrane transport
EamA PF00892 52 - 168 3.2e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30840 AT1G30840
fragaria_vesca FvH4_3g13350 FvH4_6g07670
malus_domestica MD05G1242500.v1.1 MD10G1224300.v1.1
prunus_persica Prupe.4G120900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0022031
rosa_laevigata RLG00000032639
rosa_multiflora Rmu_sc0001585.1_g000011
rosa_rugosa Rorug05G0070800
rosa_samantha Rh5AG158700 Rh5BG158000 Rh5CG172500 Rh5DG158200
rosa_wichuraiana Rw5G014140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 866
AccI GTMKAC 2 cut(s) 153, 968
AccII CGCG 5 cut(s) 137, 274, 650, 701, 905
AciI CCGC 6 cut(s) 286, 726, 1083, 1127, 1130, 1133
AclWI GGATC 2 cut(s) 20, 437
AcsI RAATTY 1 cut(s) 680
AcvI CACGTG 1 cut(s) 183
AcyI GRCGYC 1 cut(s) 863
AfaI GTAC 3 cut(s) 143, 362, 634
AfiI CCNNNNNNNGG 2 cut(s) 616, 846
AflIII ACRYGT 1 cut(s) 942
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 2 cut(s) 191, 331
AjiI CACGTC 5 cut(s) 378, 819, 843, 943, 1123
AluBI AGCT 3 cut(s) 492, 625, 826
AluI AGCT 3 cut(s) 492, 625, 826
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 2 cut(s) 20, 437
Ama87I CYCGRG 1 cut(s) 163
ApeKI GCWGC 3 cut(s) 686, 823, 836
ApoI RAATTY 1 cut(s) 680
AsiGI ACCGGT 1 cut(s) 58
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 773
AsuHPI GGTGA 5 cut(s) 685, 757, 826, 865, 950
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 1 cut(s) 773
BbrPI CACGTG 1 cut(s) 183
BbsI GAAGAC 2 cut(s) 400, 1083
BbvI GCAGC 3 cut(s) 698, 835, 848
BccI CCATC 6 cut(s) 2, 297, 646, 670, 844, 1084
BceAI ACGGC 2 cut(s) 246, 909
BciVI GTATCC 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 2 cut(s) 426, 803
BfuI GTATCC 1 cut(s) 580
BglII AGATCT 1 cut(s) 68
BisI GCNGC 5 cut(s) 687, 824, 837, 1128, 1131
BlsI GCNGC 5 cut(s) 688, 825, 838, 1129, 1132
BmcAI AGTACT 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 773
BmeT110I CYCGRG 1 cut(s) 163
BmgBI CACGTC 5 cut(s) 378, 819, 843, 943, 1123
BmgT120I GGNCC 1 cut(s) 773
BmiI GGNNCC 2 cut(s) 355, 583
BpiI GAAGAC 2 cut(s) 400, 1083
BsaAI YACGTR 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 994
BsaHI GRCGYC 1 cut(s) 863
BsaI GGTCTC 1 cut(s) 181
BsaWI WCCGGW 4 cut(s) 58, 367, 533, 875
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 616, 846
Bse118I RCCGGY 3 cut(s) 58, 168, 584
Bse1I ACTGG 1 cut(s) 675
Bse8I GATNNNNATC 1 cut(s) 994
BseGI GGATG 4 cut(s) 216, 855, 1017, 1141
BseJI GATNNNNATC 1 cut(s) 994
BseLI CCNNNNNNNGG 2 cut(s) 616, 846
BseNI ACTGG 1 cut(s) 675
BseRI GAGGAG 5 cut(s) 71, 200, 203, 380, 1049
BseXI GCAGC 3 cut(s) 698, 835, 848
Bsh1236I CGCG 5 cut(s) 137, 274, 650, 701, 905
Bsh1285I CGRYCG 1 cut(s) 579
BshTI ACCGGT 1 cut(s) 58
BsiEI CGRYCG 1 cut(s) 579
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 7 cut(s) 59, 169, 368, 534, 585, 876, 1059
BslI CCNNNNNNNGG 2 cut(s) 616, 846
BsmAI GTCTC 1 cut(s) 181
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 1 cut(s) 163
Bsp143I GATC 6 cut(s) 25, 68, 309, 429, 447, 576
Bsp68I TCGCGA 3 cut(s) 137, 701, 905
BspACI CCGC 6 cut(s) 286, 726, 1083, 1127, 1130, 1133
BspFNI CGCG 5 cut(s) 137, 274, 650, 701, 905
BspLI GGNNCC 2 cut(s) 355, 583
BspPI GGATC 2 cut(s) 20, 437
BspTNI GGTCTC 1 cut(s) 181
BsrFI RCCGGY 3 cut(s) 58, 168, 584
BsrI ACTGG 1 cut(s) 675
BssAI RCCGGY 3 cut(s) 58, 168, 584
BssMI GATC 6 cut(s) 25, 68, 309, 429, 447, 576
BssNI GRCGYC 1 cut(s) 863
Bst4CI ACNGT 7 cut(s) 158, 531, 632, 637, 694, 938, 973
Bst6I CTCTTC 4 cut(s) 33, 110, 347, 599
BstACI GRCGYC 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 3 cut(s) 199, 718, 903
BstF5I GGATG 4 cut(s) 216, 855, 1017, 1141
BstFNI CGCG 5 cut(s) 137, 274, 650, 701, 905
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 6 cut(s) 28, 71, 312, 432, 450, 579
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 6 cut(s) 25, 68, 309, 429, 447, 576
BstMCI CGRYCG 1 cut(s) 579
BstMWI GCNNNNNNNGC 3 cut(s) 280, 803, 902
BstUI CGCG 5 cut(s) 137, 274, 650, 701, 905
BstV1I GCAGC 3 cut(s) 698, 835, 848
BstV2I GAAGAC 2 cut(s) 400, 1083
BstX2I RGATCY 2 cut(s) 25, 68
BstYI RGATCY 2 cut(s) 25, 68
BsuI GTATCC 1 cut(s) 580
BtgZI GCGATG 3 cut(s) 366, 665, 920
BtrI CACGTC 5 cut(s) 378, 819, 843, 943, 1123
BtsCI GGATG 4 cut(s) 216, 855, 1017, 1141
BtuMI TCGCGA 3 cut(s) 137, 701, 905
Cac8I GCNNGC 3 cut(s) 199, 718, 903
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 3 cut(s) 58, 168, 584
Cfr13I GGNCC 1 cut(s) 773
Csp6I GTAC 3 cut(s) 142, 361, 633
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 5 cut(s) 46, 348, 658, 758, 889
CviQI GTAC 3 cut(s) 142, 361, 633
DpnI GATC 6 cut(s) 27, 70, 311, 431, 449, 578
DpnII GATC 6 cut(s) 25, 68, 309, 429, 447, 576
Eam1104I CTCTTC 4 cut(s) 33, 110, 347, 599
EarI CTCTTC 4 cut(s) 33, 110, 347, 599
EciI GGCGGA 2 cut(s) 275, 1148
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 773
Eco72I CACGTG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 163
EcoRI GAATTC 1 cut(s) 680
FaeI CATG 5 cut(s) 49, 351, 661, 761, 892
FatI CATG 5 cut(s) 45, 347, 657, 757, 888
FauI CCCGC 1 cut(s) 719
FblI GTMKAC 2 cut(s) 153, 968
Fnu4HI GCNGC 5 cut(s) 687, 824, 837, 1128, 1131
FokI GGATG 3 cut(s) 203, 862, 1024
Fsp4HI GCNGC 5 cut(s) 687, 824, 837, 1128, 1131
FspBI CTAG 2 cut(s) 426, 803
GlaI GCGC 1 cut(s) 275
GluI GCNGC 5 cut(s) 687, 824, 837, 1128, 1131
HapII CCGG 7 cut(s) 59, 169, 368, 534, 585, 876, 1059
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 863
Hin1II CATG 5 cut(s) 49, 351, 661, 761, 892
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 3 cut(s) 19, 483, 969
HindII GTYRAC 3 cut(s) 19, 483, 969
HinfI GANTC 2 cut(s) 512, 990
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 7 cut(s) 59, 169, 368, 534, 585, 876, 1059
HphI GGTGA 5 cut(s) 685, 757, 826, 865, 950
Hpy166II GTNNAC 7 cut(s) 19, 154, 375, 483, 590, 856, 969
Hpy188I TCNGA 1 cut(s) 581
Hpy188III TCNNGA 6 cut(s) 88, 136, 394, 509, 700, 904
Hpy8I GTNNAC 7 cut(s) 19, 154, 375, 483, 590, 856, 969
Hpy99I CGWCG 3 cut(s) 694, 973, 1075
HpyAV CCTTC 6 cut(s) 140, 374, 716, 1024, 1033, 1039
HpyCH4III ACNGT 7 cut(s) 158, 531, 632, 637, 694, 938, 973
HpyCH4V TGCA 2 cut(s) 667, 888
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 803, 902
Hsp92I GRCGYC 1 cut(s) 863
Hsp92II CATG 5 cut(s) 49, 351, 661, 761, 892
HspAI GCGC 1 cut(s) 274
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 6 cut(s) 25, 68, 309, 429, 447, 576
LmnI GCTCC 2 cut(s) 288, 581
Lsp1109I GCAGC 3 cut(s) 698, 835, 848
MaeI CTAG 2 cut(s) 426, 803
MaeIII GTNAC 5 cut(s) 88, 613, 644, 814, 938
MalI GATC 6 cut(s) 27, 70, 311, 431, 449, 578
MboI GATC 6 cut(s) 25, 68, 309, 429, 447, 576
MflI RGATCY 2 cut(s) 25, 68
MluCI AATT 1 cut(s) 680
MseI TTAA 8 cut(s) 18, 51, 63, 488, 539, 732, 744, 1113
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 1083
MspI CCGG 7 cut(s) 59, 169, 368, 534, 585, 876, 1059
MvnI CGCG 5 cut(s) 137, 274, 650, 701, 905
MwoI GCNNNNNNNGC 3 cut(s) 280, 803, 902
NdeII GATC 6 cut(s) 25, 68, 309, 429, 447, 576
NlaIII CATG 5 cut(s) 49, 351, 661, 761, 892
NlaIV GGNNCC 2 cut(s) 355, 583
NmuCI GTSAC 4 cut(s) 88, 613, 814, 938
NruI TCGCGA 3 cut(s) 137, 701, 905
PaeR7I CTCGAG 1 cut(s) 163
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PfeI GAWTC 2 cut(s) 512, 990
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 5 cut(s) 688, 825, 838, 1129, 1132
Ple19I CGATCG 1 cut(s) 579
PmaCI CACGTG 1 cut(s) 183
PmlI CACGTG 1 cut(s) 183
Ppu21I YACGTR 1 cut(s) 183
PspCI CACGTG 1 cut(s) 183
PspN4I GGNNCC 2 cut(s) 355, 583
PspPI GGNCC 1 cut(s) 773
PspXI VCTCGAGB 1 cut(s) 163
PsuI RGATCY 2 cut(s) 25, 68
PvuI CGATCG 1 cut(s) 579
RruI TCGCGA 3 cut(s) 137, 701, 905
RsaI GTAC 3 cut(s) 143, 362, 634
RsaNI GTAC 3 cut(s) 142, 361, 633
RseI CAYNNNNRTG 1 cut(s) 662
SalI GTCGAC 1 cut(s) 967
SaqAI TTAA 8 cut(s) 18, 51, 63, 488, 539, 732, 744, 1113
SatI GCNGC 5 cut(s) 687, 824, 837, 1128, 1131
Sau3AI GATC 6 cut(s) 25, 68, 309, 429, 447, 576
Sau96I GGNCC 1 cut(s) 773
ScaI AGTACT 1 cut(s) 143
Sfr274I CTCGAG 1 cut(s) 163
SinI GGWCC 1 cut(s) 773
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
Sse9I AATT 1 cut(s) 680
SsiI CCGC 6 cut(s) 286, 726, 1083, 1127, 1130, 1133
SspI AATATT 1 cut(s) 1055
SspMI CTAG 2 cut(s) 426, 803
TaaI ACNGT 7 cut(s) 158, 531, 632, 637, 694, 938, 973
TaqI TCGA 6 cut(s) 13, 125, 164, 395, 515, 968
TasI AATT 1 cut(s) 680
TatI WGTACW 1 cut(s) 141
TauI GCSGC 2 cut(s) 1130, 1133
TfiI GAWTC 2 cut(s) 512, 990
Tru1I TTAA 8 cut(s) 18, 51, 63, 488, 539, 732, 744, 1113
Tru9I TTAA 8 cut(s) 18, 51, 63, 488, 539, 732, 744, 1113
TseFI GTSAC 4 cut(s) 88, 613, 814, 938
TseI GCWGC 3 cut(s) 686, 823, 836
Tsp45I GTSAC 4 cut(s) 88, 613, 814, 938
TspDTI ATGAA 4 cut(s) 547, 923, 1034, 1040
TspGWI ACGGA 3 cut(s) 292, 632, 1088
VpaK11BI GGWCC 1 cut(s) 773
XapI RAATTY 1 cut(s) 680
XcmI CCANNNNNNNNNTGG 2 cut(s) 783, 847
XhoI CTCGAG 1 cut(s) 163
XmiI GTMKAC 2 cut(s) 153, 968
XspI CTAG 2 cut(s) 426, 803
ZraI GACGTC 1 cut(s) 864
ZrmI AGTACT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.