Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015201

Physical Location & Seq

Genomic Coordinates Forward (+)

32204218 .. 32205489

Gene Structure 1272 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1224300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGAGAGTTTAGTCCAATCTTCACCCCAAAACTCCCCCAAACCCCATAACCACCAACACCACCACCACCATGATCACGACAGCCCCACCCTCACCACCACCACCAACCACCACCTCCAACAAACCAGAACCAATTCATCATCATTATTCATCAATAAGACCACAGCAACCCAACTGAGTAACAAAGGCTCGATGATCAAACGCAACAAGCGCTCCATGCCTCTCCTGGTGATCAACTACTTGTGCCTCTTCATTGGATCAGTCTCTTCAAGCCTCCTCTCAAAGTACTATTTCATCCACAAAGGCTCAAGCATTTGGGTGTCCACCTGGGTTCAATGCTCCGGCTTCCCTCTCCTCCTTCCATTTATTTTCATTCCTTATTATCTCCTCCATTGCACACAAAGAAAACCCTTCTCTCACTTCACCCCCAAAATCTTAGTCCTATCAGTTCTCATAGGTCTTATGCTGGGTCTAAACAACCTCCTCTTCTCTTGGGGTAACTCCTATTTACCAGTCTCAACCTCCTCCCTCCTCCTCTCCTCACAACTCGTTTTTAATCTCATCCTCTCTGTGATTATTGTCAAGCAAAAAGTAACTTTCTCAAATCTCAACTGTGTTATCCTCCTAACCGTAAGCTCAGTCCTTCTGGCCTTGGGGTCCCACCATGACAAGCCACATGGCCTTACACGTAGCAAGTATTTCCTAGGGTTCTTCTCAACAATCGGGGCAGGCTTGTTGTTTGCTCTGTACCTCCCGGTCATGGAGAAGATATACAGCAAGGTTTACTGCTACGCCATGGTGATCGAAATGCAGCTGGTAATGGAGGCTGCGGCCACGGTGTTCGCCATGGCAGGGATGGCCTTGAACGGCGGGTTTCGTGAGATGAAGGCAGAGAGCGCGAGGGTGTATGACAAAGGAGAAAACGTGTATTGGGTGACTCTGTGGTGCAACGTTGTGACGTGGCAGCTTTGCTTCATGGGGACAGCTGGCATGGTGTTCTTGACCTCTTCGTTGACGGGTGGGATTTGTATGACGGCGCTGATAGGGATGAACGTGTTGGGAGGGGTTCTGGTGTATGGGGATTCGTTTGGGGGTGTGAAGGCGGTTTCCACTTTCTTGTGTGGGTGGGGTTTCTGTTCTTATGTGTACGGCCTTTACATGAAGGCTAGAGATATTGGGGAAGAAGATGGTGGTGAAGGAGATGGAGACAAAGAAGGAACCCACCAAGAGATAGAGATGGATGTGCATAAATTAAATGATAGTAGTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUNUT

Protein Analysis

424

Amino Acids

46.87

Weight (kDa)

8.57

Isoelectric Point (pI)

38.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUNUT	PF16913	75 - 390	1.7e-98	Purine nucleobase transmembrane transport
EamA	PF00892	87 - 216	2.9e-07	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015201)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30840 AT1G30840
fragaria_vesca	FvH4_3g13350 FvH4_6g07670
malus_domestica	MD05G1242500.v1.1 MD10G1224300.v1.1
prunus_persica	Prupe.4G120900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0022031
rosa_laevigata	RLG00000032639
rosa_multiflora	Rmu_sc0001585.1_g000011
rosa_rugosa	Rorug05G0070800
rosa_samantha	Rh5AG158700 Rh5BG158000 Rh5CG172500 Rh5DG158200
rosa_wichuraiana	Rw5G014140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	899
AciI	CCGC	3 cut(s)	830, 870, 1103
AclI	AACGTT	1 cut(s)	951
AclWI	GGATC	1 cut(s)	265
AcoI	YGGCCR	1 cut(s)	831
AfaI	GTAC	3 cut(s)	287, 749, 1148
AfeI	AGCGCT	1 cut(s)	212
AfiI	CCNNNNNNNGG	2 cut(s)	760, 852
AflIII	ACRYGT	3 cut(s)	686, 924, 1053
AgsI	TTSAA	3 cut(s)	270, 335, 865
AjiI	CACGTC	1 cut(s)	960
AjnI	CCWGG	2 cut(s)	225, 326
AluBI	AGCT	4 cut(s)	636, 814, 967, 986
AluI	AGCT	4 cut(s)	636, 814, 967, 986
Alw26I	GTCTC	3 cut(s)	268, 520, 1200
AlwI	GGATC	1 cut(s)	265
Aor51HI	AGCGCT	1 cut(s)	212
AoxI	GGCC	5 cut(s)	648, 679, 831, 858, 1150
ApeKI	GCWGC	3 cut(s)	811, 827, 964
ArsI	GACNNNNNNTTYG	2 cut(s)	423, 455
Asp700I	GAANNNNTTC	1 cut(s)	133
AspA2I	CCTAGG	1 cut(s)	703
AspLEI	GCGC	3 cut(s)	213, 899, 1039
AspS9I	GGNCC	1 cut(s)	657
AsuC2I	CCSGG	1 cut(s)	755
AsuHPI	GGTGA	7 cut(s)	15, 85, 241, 415, 811, 946, 1205
AvaII	GGWCC	1 cut(s)	657
AvrII	CCTAGG	1 cut(s)	703
BbvI	GCAGC	3 cut(s)	814, 823, 976
BccI	CCATC	4 cut(s)	850, 1181, 1196, 1231
BceAI	ACGGC	3 cut(s)	883, 1050, 1165
BciT130I	CCWGG	2 cut(s)	227, 328
BclI	TGATCA	3 cut(s)	73, 195, 231
BcnI	CCSGG	1 cut(s)	755
BcoDI	GTCTC	3 cut(s)	268, 520, 1200
BfaI	CTAG	2 cut(s)	704, 1167
BfoI	RGCGCY	2 cut(s)	214, 1040
BisI	GCNGC	4 cut(s)	812, 828, 831, 965
BlnI	CCTAGG	1 cut(s)	703
BlsI	GCNGC	4 cut(s)	813, 829, 832, 966
BmcAI	AGTACT	1 cut(s)	287
Bme1390I	CCNGG	3 cut(s)	227, 328, 755
Bme18I	GGWCC	1 cut(s)	657
BmgBI	CACGTC	1 cut(s)	960
BmgT120I	GGNCC	1 cut(s)	657
BmiI	GGNNCC	3 cut(s)	658, 659, 1219
BmrFI	CCNGG	3 cut(s)	227, 328, 755
BpuEI	CTTGAG	1 cut(s)	292
BpuMI	CCSGG	1 cut(s)	755
BsaAI	YACGTR	1 cut(s)	689
BsaJI	CCNNGG	6 cut(s)	327, 651, 703, 795, 834, 846
BsaXI	ACNNNNNCTCC	4 cut(s)	197, 227, 909, 939
Bsc4I	CCNNNNNNNGG	2 cut(s)	760, 852
Bse1I	ACTGG	1 cut(s)	512
Bse3DI	GCAATG	1 cut(s)	391
BseBI	CCWGG	2 cut(s)	227, 328
BseDI	CCNNGG	6 cut(s)	327, 651, 703, 795, 834, 846
BseGI	GGATG	5 cut(s)	294, 561, 861, 1053, 1246
BseLI	CCNNNNNNNGG	2 cut(s)	760, 852
BseMI	GCAATG	1 cut(s)	391
BseMII	CTCAG	2 cut(s)	167, 651
BseNI	ACTGG	1 cut(s)	512
BseRI	GAGGAG	8 cut(s)	266, 344, 377, 473, 514, 521, 524, 529
BseXI	GCAGC	3 cut(s)	814, 823, 976
BseYI	CCCAGC	1 cut(s)	466
Bsh1236I	CGCG	1 cut(s)	899
BshFI	GGCC	5 cut(s)	650, 681, 833, 860, 1152
BsiSI	CCGG	2 cut(s)	342, 755
BslFI	GGGAC	2 cut(s)	643, 994
BslI	CCNNNNNNNGG	2 cut(s)	760, 852
BsmAI	GTCTC	3 cut(s)	268, 520, 1200
BsmFI	GGGAC	2 cut(s)	643, 994
BsnI	GGCC	5 cut(s)	650, 681, 833, 860, 1152
Bsp143I	GATC	5 cut(s)	73, 195, 231, 257, 801
Bsp19I	CCATGG	2 cut(s)	795, 846
BspACI	CCGC	3 cut(s)	830, 870, 1103
BspANI	GGCC	5 cut(s)	650, 681, 833, 860, 1152
BspCNI	CTCAG	2 cut(s)	168, 650
BspFNI	CGCG	1 cut(s)	899
BspLI	GGNNCC	3 cut(s)	658, 659, 1219
BspPI	GGATC	1 cut(s)	265
BsrDI	GCAATG	1 cut(s)	391
BsrI	ACTGG	1 cut(s)	512
BssECI	CCNNGG	6 cut(s)	327, 651, 703, 795, 834, 846
BssMI	GATC	5 cut(s)	73, 195, 231, 257, 801
BssT1I	CCWWGG	4 cut(s)	651, 703, 795, 846
Bst2UI	CCWGG	2 cut(s)	227, 328
Bst4CI	ACNGT	3 cut(s)	614, 631, 838
Bst6I	CTCTTC	4 cut(s)	254, 271, 491, 1012
BstBAI	YACGTR	1 cut(s)	689
BstC8I	GCNNGC	2 cut(s)	730, 988
BstDEI	CTNAG	3 cut(s)	176, 436, 637
BstDSI	CCRYGG	3 cut(s)	795, 834, 846
BstF5I	GGATG	5 cut(s)	294, 561, 861, 1053, 1246
BstFNI	CGCG	1 cut(s)	899
BstH2I	RGCGCY	2 cut(s)	214, 1040
BstHHI	GCGC	3 cut(s)	213, 899, 1039
BstKTI	GATC	5 cut(s)	76, 198, 234, 260, 804
BstMAI	GTCTC	3 cut(s)	268, 520, 1200
BstMBI	GATC	5 cut(s)	73, 195, 231, 257, 801
BstMWI	GCNNNNNNNGC	4 cut(s)	210, 217, 857, 896
BstNI	CCWGG	2 cut(s)	227, 328
BstSCI	CCNGG	3 cut(s)	225, 326, 753
BstUI	CGCG	1 cut(s)	899
BstV1I	GCAGC	3 cut(s)	814, 823, 976
BsuRI	GGCC	5 cut(s)	650, 681, 833, 860, 1152
BtgI	CCRYGG	3 cut(s)	795, 834, 846
BtrI	CACGTC	1 cut(s)	960
BtsCI	GGATG	5 cut(s)	294, 561, 861, 1053, 1246
Cac8I	GCNNGC	2 cut(s)	730, 988
CfoI	GCGC	3 cut(s)	213, 899, 1039
Cfr13I	GGNCC	1 cut(s)	657
Csp6I	GTAC	3 cut(s)	286, 748, 1147
CviQI	GTAC	3 cut(s)	286, 748, 1147
DdeI	CTNAG	3 cut(s)	176, 436, 637
DpnI	GATC	5 cut(s)	75, 197, 233, 259, 803
DpnII	GATC	5 cut(s)	73, 195, 231, 257, 801
EaeI	YGGCCR	1 cut(s)	831
Eam1104I	CTCTTC	4 cut(s)	254, 271, 491, 1012
EarI	CTCTTC	4 cut(s)	254, 271, 491, 1012
Eco130I	CCWWGG	4 cut(s)	651, 703, 795, 846
Eco47I	GGWCC	1 cut(s)	657
Eco47III	AGCGCT	1 cut(s)	212
EcoO109I	RGGNCCY	1 cut(s)	657
EcoRII	CCWGG	2 cut(s)	225, 326
EcoT14I	CCWWGG	4 cut(s)	651, 703, 795, 846
ErhI	CCWWGG	4 cut(s)	651, 703, 795, 846
FaqI	GGGAC	2 cut(s)	643, 994
FauI	CCCGC	1 cut(s)	863
FbaI	TGATCA	3 cut(s)	73, 195, 231
Fnu4HI	GCNGC	4 cut(s)	812, 828, 831, 965
FokI	GGATG	5 cut(s)	281, 548, 868, 1060, 1253
Fsp4HI	GCNGC	4 cut(s)	812, 828, 831, 965
FspBI	CTAG	2 cut(s)	704, 1167
GlaI	GCGC	3 cut(s)	212, 898, 1038
GluI	GCNGC	4 cut(s)	812, 828, 831, 965
GsaI	CCCAGC	1 cut(s)	470
HaeII	RGCGCY	2 cut(s)	214, 1040
HaeIII	GGCC	5 cut(s)	650, 681, 833, 860, 1152
HapII	CCGG	2 cut(s)	342, 755
HhaI	GCGC	3 cut(s)	213, 899, 1039
Hin6I	GCGC	3 cut(s)	211, 897, 1037
HinP1I	GCGC	3 cut(s)	211, 897, 1037
HincII	GTYRAC	1 cut(s)	1014
HindII	GTYRAC	1 cut(s)	1014
HinfI	GANTC	2 cut(s)	937, 1082
HpaII	CCGG	2 cut(s)	342, 755
HphI	GGTGA	7 cut(s)	15, 85, 241, 415, 811, 946, 1205
Hpy166II	GTNNAC	4 cut(s)	324, 784, 1014, 1147
Hpy188III	TCNNGA	3 cut(s)	77, 878, 1000
Hpy8I	GTNNAC	4 cut(s)	324, 784, 1014, 1147
HpyAV	CCTTC	8 cut(s)	368, 421, 653, 880, 1093, 1156, 1190, 1208
HpyCH4III	ACNGT	3 cut(s)	614, 631, 838
HpyCH4IV	ACGT	5 cut(s)	688, 924, 951, 959, 1053
HpyCH4V	TGCA	4 cut(s)	396, 811, 948, 1246
HpyF10VI	GCNNNNNNNGC	4 cut(s)	210, 217, 857, 896
HpyF3I	CTNAG	3 cut(s)	176, 436, 637
HpySE526I	ACGT	5 cut(s)	688, 924, 951, 959, 1053
HspAI	GCGC	3 cut(s)	211, 897, 1037
KflI	GGGWCCC	1 cut(s)	657
Ksp22I	TGATCA	3 cut(s)	73, 195, 231
Kzo9I	GATC	5 cut(s)	73, 195, 231, 257, 801
LmnI	GCTCC	2 cut(s)	218, 344
Lsp1109I	GCAGC	3 cut(s)	814, 823, 976
MaeI	CTAG	2 cut(s)	704, 1167
MaeII	ACGT	5 cut(s)	688, 924, 951, 959, 1053
MaeIII	GTNAC	5 cut(s)	179, 497, 592, 934, 955
MalI	GATC	5 cut(s)	75, 197, 233, 259, 803
MboI	GATC	5 cut(s)	73, 195, 231, 257, 801
MboII	GAAGA	9 cut(s)	12, 241, 258, 478, 703, 778, 999, 1193, 1196
MluCI	AATT	2 cut(s)	133, 1250
MlyI	GAGTC	1 cut(s)	931
MmeI	TCCRAC	1 cut(s)	142
MroXI	GAANNNNTTC	1 cut(s)	133
MseI	TTAA	2 cut(s)	555, 1253
MslI	CAYNNNNRTG	2 cut(s)	69, 317
MspA1I	CMGCKG	2 cut(s)	814, 986
MspI	CCGG	2 cut(s)	342, 755
MspR9I	CCNGG	3 cut(s)	227, 328, 755
MvaI	CCWGG	2 cut(s)	227, 328
MvnI	CGCG	1 cut(s)	899
MwoI	GCNNNNNNNGC	4 cut(s)	210, 217, 857, 896
NciI	CCSGG	1 cut(s)	755
NcoI	CCATGG	2 cut(s)	795, 846
NdeII	GATC	5 cut(s)	73, 195, 231, 257, 801
NlaIV	GGNNCC	3 cut(s)	658, 659, 1219
NmuCI	GTSAC	2 cut(s)	934, 955
PdmI	GAANNNNTTC	1 cut(s)	133
PfeI	GAWTC	1 cut(s)	1082
PkrI	GCNGC	4 cut(s)	813, 829, 832, 966
PleI	GAGTC	1 cut(s)	931
PpsI	GAGTC	1 cut(s)	931
Ppu21I	YACGTR	1 cut(s)	689
PpuMI	RGGWCCY	1 cut(s)	657
Psp1406I	AACGTT	1 cut(s)	951
Psp5II	RGGWCCY	1 cut(s)	657
Psp6I	CCWGG	2 cut(s)	225, 326
PspFI	CCCAGC	1 cut(s)	466
PspGI	CCWGG	2 cut(s)	225, 326
PspN4I	GGNNCC	3 cut(s)	658, 659, 1219
PspPI	GGNCC	1 cut(s)	657
PspPPI	RGGWCCY	1 cut(s)	657
PvuII	CAGCTG	2 cut(s)	814, 986
RsaI	GTAC	3 cut(s)	287, 749, 1148
RsaNI	GTAC	3 cut(s)	286, 748, 1147
RseI	CAYNNNNRTG	2 cut(s)	69, 317
SaqAI	TTAA	2 cut(s)	555, 1253
SatI	GCNGC	4 cut(s)	812, 828, 831, 965
Sau3AI	GATC	5 cut(s)	73, 195, 231, 257, 801
Sau96I	GGNCC	1 cut(s)	657
ScaI	AGTACT	1 cut(s)	287
SchI	GAGTC	1 cut(s)	931
ScrFI	CCNGG	3 cut(s)	227, 328, 755
SinI	GGWCC	1 cut(s)	657
SmiMI	CAYNNNNRTG	2 cut(s)	69, 317
SmlI	CTYRAG	1 cut(s)	307
SmoI	CTYRAG	1 cut(s)	307
Sse9I	AATT	2 cut(s)	133, 1250
SsiI	CCGC	3 cut(s)	830, 870, 1103
SspMI	CTAG	2 cut(s)	704, 1167
StyD4I	CCNGG	3 cut(s)	225, 326, 753
StyI	CCWWGG	4 cut(s)	651, 703, 795, 846
TaaI	ACNGT	3 cut(s)	614, 631, 838
TaiI	ACGT	5 cut(s)	691, 927, 954, 962, 1056
TaqI	TCGA	2 cut(s)	191, 804
TasI	AATT	2 cut(s)	133, 1250
TatI	WGTACW	1 cut(s)	285
TauI	GCSGC	1 cut(s)	833
TfiI	GAWTC	1 cut(s)	1082
Tru1I	TTAA	2 cut(s)	555, 1253
Tru9I	TTAA	2 cut(s)	555, 1253
TseFI	GTSAC	2 cut(s)	934, 955
TseI	GCWGC	3 cut(s)	811, 827, 964
Tsp45I	GTSAC	2 cut(s)	934, 955
TspDTI	ATGAA	9 cut(s)	126, 139, 241, 283, 361, 899, 964, 1064, 1175
VpaK11BI	GGWCC	1 cut(s)	657
XcmI	CCANNNNNNNNNTGG	2 cut(s)	223, 853
XmaJI	CCTAGG	1 cut(s)	703
XmnI	GAANNNNTTC	1 cut(s)	133
XspI	CTAG	2 cut(s)	704, 1167
ZrmI	AGTACT	1 cut(s)	287

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.