FvH4_3g13350

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015201
Physical Location & Seq
Reverse (-)
7978430 .. 7979521
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13350.t1

Sequence Viewer

Length: 1092 bp
ATGCCTCCCTCCGCCGATATGACCCCCCACACTCCCAAAAACAAAGGCTCTCTCACCTTGTTGGTCCTAAACTACCTTCTTCTCTTCGTTGGCTCAGTTTCAGCCACCCTCCTCTCTAAATACTATTTCATTCATAAAGGCTCAAACATTTGGCTTGCTACATGGGTTCAGTGTGCTGGCTTTCCTCTTCTTCTCCCCCCAATTCTCCTCCCTTATTTCCTCAAACTCACCACCAGAAGACCTTTCACCCAATTTACCTCCAAAATTTTCTCCATCTCCATTTTCGTTGGCCTAATGTTGGGACTCAACAACCTCCTCTTCTCTCTCGGCAACTCCAACCTCCCGGTCTCCACCTCCTCCCTCCTCCTCTCTTCACAACTCGTCTTCAACCTCATCCTCTCCGTTCTCATCGTCAAACAGAAGGTCACATTCTCCAACCTCAACTGCGTCATCCTCCTAACCGTCAGCTCTATTCTCTTAGCCCTTGGCTCGAGCCACGACAAGCCTCCCGGTCTAACACGTGGAAAGTACTTCGTCGGATTCTTCTCCACCGTCGGGGCCGGCTTGCTCTTTGCTCTCTACCTCCCCGTCATGGAAAAAGTCTACAGAAAAGTTGACTGCTATTCCATGATGATGGAAATGCAGGTGGCGATGGAGGCAGCAGCGACGGCGCTGTCGACGGTGTTGATGGTGTCGAACGGTGCGTTTGGTGGCATGGTGAGGGAGGGGAGGGAGGTGTTTGACAAGGGAGAGACGCTGTATTGGGTGACGGTGGTGTTTAACGTCGTGACATGGCAGCTGTGCTTTATGGGGACGGCGGGGATGGTTTTCTTGACGACGTCGCTGACCGGAGGGATATGCATGACGGCGTTGATGGGGATAAACGTGTTGGGGGGAGTGGTGGTTTACGGGGACAAGTTTGGTGGGGTGAAAGTGGTTTCGACTGTGCTCTGTGTATGGGGGTTTTGTTCTTATGTGTACGGGATTTACATCAAGTCGAAGAAGATTAGGGAGGAGGAGGACGAGGACCGCACTGAGATGATAGAGATGAGTAGAAGTCCAAGTGTGCATAACGGTGAAAATAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

364

Amino Acids

39.82

Weight (kDa)

8.97

Isoelectric Point (pI)

25.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 20 - 335 1.3e-95 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30840 AT1G30840
fragaria_vesca FvH4_3g13350 FvH4_6g07670
malus_domestica MD05G1242500.v1.1 MD10G1224300.v1.1
prunus_persica Prupe.4G120900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0022031
rosa_laevigata RLG00000032639
rosa_multiflora Rmu_sc0001585.1_g000011
rosa_rugosa Rorug05G0070800
rosa_samantha Rh5AG158700 Rh5BG158000 Rh5CG172500 Rh5DG158200
rosa_wichuraiana Rw5G014140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 634
AasI GACNNNNNNGTC 1 cut(s) 673
AatII GACGTC 1 cut(s) 842
Acc36I ACCTGC 1 cut(s) 634
AccI GTMKAC 2 cut(s) 603, 677
AciI CCGC 3 cut(s) 12, 818, 1030
AcsI RAATTY 1 cut(s) 264
AcvI CACGTG 1 cut(s) 521
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 2 cut(s) 530, 980
AfiI CCNNNNNNNGG 3 cut(s) 298, 555, 592
AflIII ACRYGT 2 cut(s) 518, 885
AgsI TTSAA 1 cut(s) 388
AluBI AGCT 2 cut(s) 468, 799
AluI AGCT 2 cut(s) 468, 799
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 2 cut(s) 352, 746
Ama87I CYCGRG 1 cut(s) 490
AoxI GGCC 2 cut(s) 289, 558
ApeKI GCWGC 3 cut(s) 659, 662, 796
ApoI RAATTY 1 cut(s) 264
ArsI GACNNNNNNTTYG 2 cut(s) 408, 440
AspLEI GCGC 1 cut(s) 673
AspS9I GGNCC 3 cut(s) 64, 558, 1027
AsuC2I CCSGG 2 cut(s) 344, 510
AsuHPI GGTGA 7 cut(s) 46, 220, 238, 730, 778, 940, 1088
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 2 cut(s) 64, 1027
BbrPI CACGTG 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 244, 376
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 3 cut(s) 671, 674, 808
BccI CCATC 6 cut(s) 281, 628, 646, 682, 817, 868
BceAI ACGGC 3 cut(s) 684, 831, 882
BcnI CCSGG 2 cut(s) 344, 510
BcoDI GTCTC 2 cut(s) 352, 746
BfmI CTRYAG 1 cut(s) 604
BfoI RGCGCY 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 634
BisI GCNGC 3 cut(s) 660, 663, 797
BlsI GCNGC 3 cut(s) 661, 664, 798
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 344, 510
Bme18I GGWCC 2 cut(s) 64, 1027
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 3 cut(s) 64, 558, 1027
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 2 cut(s) 344, 510
BpiI GAAGAC 2 cut(s) 244, 376
BpuMI CCSGG 2 cut(s) 344, 510
BsaAI YACGTR 1 cut(s) 521
BsaBI GATNNNNATC 1 cut(s) 989
BsaHI GRCGYC 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 1 cut(s) 484
BsaWI WCCGGW 1 cut(s) 848
BsaXI ACNNNNNCTCC 2 cut(s) 888, 918
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 555, 592
Bse118I RCCGGY 1 cut(s) 560
Bse8I GATNNNNATC 1 cut(s) 989
BseDI CCNNGG 1 cut(s) 484
BseGI GGATG 3 cut(s) 393, 450, 828
BseJI GATNNNNATC 1 cut(s) 989
BseLI CCNNNNNNNGG 3 cut(s) 298, 555, 592
BseMII CTCAG 2 cut(s) 108, 1026
BseRI GAGGAG 8 cut(s) 101, 197, 305, 346, 353, 356, 1028, 1031
BseXI GCAGC 3 cut(s) 671, 674, 808
BshFI GGCC 2 cut(s) 291, 560
BsiHKAI GWGCWC 1 cut(s) 951
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 4 cut(s) 344, 510, 561, 849
BslFI GGGAC 3 cut(s) 315, 826, 926
BslI CCNNNNNNNGG 3 cut(s) 298, 555, 592
BsmAI GTCTC 2 cut(s) 352, 746
BsmBI CGTCTC 1 cut(s) 746
BsmFI GGGAC 3 cut(s) 315, 826, 926
BsnI GGCC 2 cut(s) 291, 560
Bso31I GGTCTC 1 cut(s) 352
BsoBI CYCGRG 1 cut(s) 490
Bsp1286I GDGCHC 1 cut(s) 951
BspACI CCGC 3 cut(s) 12, 818, 1030
BspANI GGCC 2 cut(s) 291, 560
BspCNI CTCAG 2 cut(s) 107, 1027
BspLI GGNNCC 1 cut(s) 559
BspMI ACCTGC 1 cut(s) 634
BspTNI GGTCTC 1 cut(s) 352
BsrFI RCCGGY 1 cut(s) 560
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 1 cut(s) 484
BssNI GRCGYC 1 cut(s) 839
BssT1I CCWWGG 1 cut(s) 484
Bst4CI ACNGT 7 cut(s) 463, 553, 682, 701, 772, 946, 1076
Bst6I CTCTTC 4 cut(s) 89, 192, 323, 376
BstACI GRCGYC 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 156, 178, 562, 566
BstDEI CTNAG 3 cut(s) 94, 478, 1035
BstF5I GGATG 3 cut(s) 393, 450, 828
BstH2I RGCGCY 1 cut(s) 674
BstHHI GCGC 1 cut(s) 673
BstMAI GTCTC 2 cut(s) 352, 746
BstMWI GCNNNNNNNGC 2 cut(s) 656, 668
BstSCI CCNGG 2 cut(s) 342, 508
BstSFI CTRYAG 1 cut(s) 604
BstV1I GCAGC 3 cut(s) 671, 674, 808
BstV2I GAAGAC 2 cut(s) 244, 376
BstXI CCANNNNNNTGG 1 cut(s) 634
BsuRI GGCC 2 cut(s) 291, 560
BtgZI GCGATG 1 cut(s) 665
BtsCI GGATG 3 cut(s) 393, 450, 828
BtsIMutI CAGTG 2 cut(s) 176, 1032
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 4 cut(s) 156, 178, 562, 566
CfoI GCGC 1 cut(s) 673
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 3 cut(s) 64, 558, 1027
CseI GACGC 2 cut(s) 436, 763
Csp6I GTAC 2 cut(s) 529, 979
CspCI CAANNNNNGTGG 2 cut(s) 904, 939
CviAII CATG 6 cut(s) 162, 592, 628, 715, 792, 862
CviQI GTAC 2 cut(s) 529, 979
DdeI CTNAG 3 cut(s) 94, 478, 1035
DrdI GACNNNNNNGTC 1 cut(s) 673
DseDI GACNNNNNNGTC 1 cut(s) 673
Eam1104I CTCTTC 4 cut(s) 89, 192, 323, 376
EarI CTCTTC 4 cut(s) 89, 192, 323, 376
Eco130I CCWWGG 1 cut(s) 484
Eco31I GGTCTC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 64, 1027
Eco72I CACGTG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 1 cut(s) 863
ErhI CCWWGG 1 cut(s) 484
Esp3I CGTCTC 1 cut(s) 746
FaeI CATG 6 cut(s) 165, 595, 631, 718, 795, 865
FaqI GGGAC 3 cut(s) 315, 826, 926
FatI CATG 6 cut(s) 161, 591, 627, 714, 791, 861
FauI CCCGC 1 cut(s) 811
FblI GTMKAC 2 cut(s) 603, 677
Fnu4HI GCNGC 3 cut(s) 660, 663, 797
FokI GGATG 3 cut(s) 380, 437, 835
Fsp4HI GCNGC 3 cut(s) 660, 663, 797
GlaI GCGC 1 cut(s) 672
GluI GCNGC 3 cut(s) 660, 663, 797
HaeII RGCGCY 1 cut(s) 674
HaeIII GGCC 2 cut(s) 291, 560
HapII CCGG 4 cut(s) 344, 510, 561, 849
HgaI GACGC 2 cut(s) 436, 763
HhaI GCGC 1 cut(s) 673
Hin1I GRCGYC 1 cut(s) 839
Hin1II CATG 6 cut(s) 165, 595, 631, 718, 795, 865
Hin6I GCGC 1 cut(s) 671
HinP1I GCGC 1 cut(s) 671
HincII GTYRAC 2 cut(s) 616, 678
HindII GTYRAC 2 cut(s) 616, 678
HinfI GANTC 2 cut(s) 303, 540
HpaII CCGG 4 cut(s) 344, 510, 561, 849
HphI GGTGA 7 cut(s) 46, 220, 238, 730, 778, 940, 1088
Hpy166II GTNNAC 5 cut(s) 604, 616, 678, 907, 979
Hpy188I TCNGA 1 cut(s) 539
Hpy188III TCNNGA 2 cut(s) 787, 832
Hpy8I GTNNAC 5 cut(s) 604, 616, 678, 907, 979
Hpy99I CGWCG 7 cut(s) 539, 557, 670, 682, 788, 841, 844
HpyAV CCTTC 2 cut(s) 86, 415
HpyCH4III ACNGT 7 cut(s) 463, 553, 682, 701, 772, 946, 1076
HpyCH4IV ACGT 4 cut(s) 520, 783, 839, 885
HpyCH4V TGCA 3 cut(s) 643, 861, 1069
HpyF10VI GCNNNNNNNGC 2 cut(s) 656, 668
HpyF3I CTNAG 3 cut(s) 94, 478, 1035
HpySE526I ACGT 4 cut(s) 520, 783, 839, 885
Hsp92I GRCGYC 1 cut(s) 839
Hsp92II CATG 6 cut(s) 165, 595, 631, 718, 795, 865
HspAI GCGC 1 cut(s) 671
KroI GCCGGC 1 cut(s) 560
KroNI GCCGGC 1 cut(s) 562
LpnPI CCDG 7 cut(s) 162, 247, 357, 523, 574, 629, 862
Lsp1109I GCAGC 3 cut(s) 671, 674, 808
MaeII ACGT 4 cut(s) 520, 783, 839, 885
MaeIII GTNAC 3 cut(s) 424, 766, 787
MhlI GDGCHC 1 cut(s) 951
MluCI AATT 3 cut(s) 201, 251, 264
MlyI GAGTC 1 cut(s) 297
MmeI TCCRAC 3 cut(s) 360, 459, 517
Mph1103I ATGCAT 1 cut(s) 863
MroNI GCCGGC 1 cut(s) 560
MseI TTAA 1 cut(s) 780
MslI CAYNNNNRTG 3 cut(s) 632, 1037, 1074
MspA1I CMGCKG 1 cut(s) 799
MspI CCGG 4 cut(s) 344, 510, 561, 849
MspR9I CCNGG 2 cut(s) 344, 510
MwoI GCNNNNNNNGC 2 cut(s) 656, 668
NaeI GCCGGC 1 cut(s) 562
NciI CCSGG 2 cut(s) 344, 510
NgoMIV GCCGGC 1 cut(s) 560
NlaIII CATG 6 cut(s) 165, 595, 631, 718, 795, 865
NlaIV GGNNCC 1 cut(s) 559
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 3 cut(s) 424, 766, 787
NsiI ATGCAT 1 cut(s) 863
PaeR7I CTCGAG 1 cut(s) 490
PaqCI CACCTGC 1 cut(s) 634
PcsI WCGNNNNNNNCGW 2 cut(s) 674, 701
PdiI GCCGGC 1 cut(s) 562
PfeI GAWTC 1 cut(s) 540
PflFI GACNNNGTC 1 cut(s) 838
PkrI GCNGC 3 cut(s) 661, 664, 798
PleI GAGTC 1 cut(s) 297
PmaCI CACGTG 1 cut(s) 521
PmlI CACGTG 1 cut(s) 521
PpsI GAGTC 1 cut(s) 297
Ppu21I YACGTR 1 cut(s) 521
PspCI CACGTG 1 cut(s) 521
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 3 cut(s) 64, 558, 1027
PspXI VCTCGAGB 1 cut(s) 490
PsyI GACNNNGTC 1 cut(s) 838
PvuII CAGCTG 1 cut(s) 799
RsaI GTAC 2 cut(s) 530, 980
RsaNI GTAC 2 cut(s) 529, 979
RseI CAYNNNNRTG 3 cut(s) 632, 1037, 1074
SalI GTCGAC 1 cut(s) 676
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 3 cut(s) 660, 663, 797
Sau96I GGNCC 3 cut(s) 64, 558, 1027
ScaI AGTACT 1 cut(s) 530
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 2 cut(s) 344, 510
SduI GDGCHC 1 cut(s) 951
SfcI CTRYAG 1 cut(s) 604
Sfr274I CTCGAG 1 cut(s) 490
SinI GGWCC 2 cut(s) 64, 1027
SlaI CTCGAG 1 cut(s) 490
SmiMI CAYNNNNRTG 3 cut(s) 632, 1037, 1074
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
Sse9I AATT 3 cut(s) 201, 251, 264
SsiI CCGC 3 cut(s) 12, 818, 1030
StyD4I CCNGG 2 cut(s) 342, 508
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 7 cut(s) 463, 553, 682, 701, 772, 946, 1076
TaiI ACGT 4 cut(s) 523, 786, 842, 888
TaqI TCGA 5 cut(s) 491, 677, 695, 941, 998
TasI AATT 3 cut(s) 201, 251, 264
TatI WGTACW 1 cut(s) 528
TfiI GAWTC 1 cut(s) 540
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 2 cut(s) 176, 1039
TseFI GTSAC 3 cut(s) 424, 766, 787
TseI GCWGC 3 cut(s) 659, 662, 796
Tsp45I GTSAC 3 cut(s) 424, 766, 787
TspDTI ATGAA 2 cut(s) 118, 122
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 2 cut(s) 176, 1039
Tth111I GACNNNGTC 1 cut(s) 838
VpaK11BI GGWCC 2 cut(s) 64, 1027
XapI RAATTY 1 cut(s) 264
XhoI CTCGAG 1 cut(s) 490
XmiI GTMKAC 2 cut(s) 603, 677
ZraI GACGTC 1 cut(s) 840
ZrmI AGTACT 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.