Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0015201

Physical Location & Seq

Genomic Coordinates Forward (+)

37529472 .. 37530728

Gene Structure 1257 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1242500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGGAGAGTGTAGTCCAAACTTCACCCATGAACTCCCCCAATCCCCATAACCACCATGATCATGGCTGCCCCACCCTCATCACCACCAACCACCACATCCCACAAACCAGAACCAATTCATCATCATTCTTGATCAACAACAACACCACAACACAACTGAGCAACAAAGGCTTGATGATCAAACCCAACAAGCGCTCCATGCCTCTCCTTGTGATCAATTACTTGTGCCTCTTCATTGGATCAGTCTCTTCAAGCCTGCTCTCAAAGTACTATTTCATCCACAAAGGCTCAAGCATTTTGGTGTCAACGTGGGTTCAATGCTCTGGCTTCCCTCTCCTCCTTCCATTTATTTTCATTCCTTATTATCTCCTCCATTGCACCCAAAGAAAACCCTTCTCTCACTTCACCCCCAAAATCTTAGTCCTGTCAATTCTCATAGGTCTTATGCTGGGTCTAAACAACTTCCTCTTCTCTTGGGGTAACTCCTATTTACCAGTCTCCACCTCCTCCCTCCTCCTCTCCTCCCAACTTGTTTTCAATCTCATCCTCTCTGTAATTATTGTCAAGCAAAAAGTAACTTTCTCAAATCTCAATTGTGTTATCCTCCTCACCCTAAGCTCAGTCCTACTGGCTTTGGGGTCCAACCATGACAAGCCACATGGCCTGACACGTGGCAAGTATTTCTTAGGGTTCTTCTCAACAATTGGGGCAGGCTTGCTGTTTGCTCTGTATCTCCCGGTCATGGAGAAGATATACAGCAAGGTTTACTGCTACGCCATGGTGATCGAAATGCAGCTGGTGATGGAGGCTGCGGCCACGGTGTTGGCCACGGTCGGGATGGCCTTGAAAGGCGGGTTTCGCGAGATGAAGGTGGAGAGCGCGAGGGTGTTTGACAAAGGAGAAAACGTGTATTGGGTGACCGTGTGGTGCAACGTGGTGACGTGGCAGCTTTGCTTCATGGGGACAGCCGGCATGGTGTTCTTAACCTCTTCGTTGACCGGTGGGATTTGCATGACGGCGTTGATGGGGATGAATGTGTTGGGAGGCGTTTTGGTGTACGGGGATTCGTTTGGGGGCGTGAAGGTGGTTTCCACTTTCTTGTGTGGATGGGGTTTCTGTTCTTATGTATACGGCCTTTACATCAAAGCTAGAGATATTAGGGAAGAAGGCGGTGGTGAAGGAGGAGATGGAGACAAAGAAGGAACCCACCAAGAGATAGAGATGGATGTGCATAAAGTAAATGATAGTAGTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUNUT

Protein Analysis

419

Amino Acids

46.04

Weight (kDa)

8.66

Isoelectric Point (pI)

30.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUNUT	PF16913	69 - 384	2.5e-97	Purine nucleobase transmembrane transport
EamA	PF00892	81 - 210	1.8e-06	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015201)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30840 AT1G30840
fragaria_vesca	FvH4_3g13350 FvH4_6g07670
malus_domestica	MD05G1242500.v1.1 MD10G1224300.v1.1
prunus_persica	Prupe.4G120900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0022031
rosa_laevigata	RLG00000032639
rosa_multiflora	Rmu_sc0001585.1_g000011
rosa_rugosa	Rorug05G0070800
rosa_samantha	Rh5AG158700 Rh5BG158000 Rh5CG172500 Rh5DG158200
rosa_wichuraiana	Rw5G014140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1128
AccII	CGCG	2 cut(s)	861, 881
AciI	CCGC	3 cut(s)	812, 852, 1170
AclWI	GGATC	1 cut(s)	247
AcoI	YGGCCR	2 cut(s)	813, 825
AcvI	CACGTG	1 cut(s)	671
AfaI	GTAC	2 cut(s)	269, 1058
AfeI	AGCGCT	1 cut(s)	194
AfiI	CCNNNNNNNGG	2 cut(s)	742, 834
AflIII	ACRYGT	2 cut(s)	668, 906
AgeI	ACCGGT	1 cut(s)	998
AgsI	TTSAA	4 cut(s)	252, 317, 538, 847
AjiI	CACGTC	1 cut(s)	942
AluBI	AGCT	4 cut(s)	618, 796, 949, 1148
AluI	AGCT	4 cut(s)	618, 796, 949, 1148
Alw26I	GTCTC	3 cut(s)	250, 502, 1185
AlwI	GGATC	1 cut(s)	247
Aor51HI	AGCGCT	1 cut(s)	194
AoxI	GGCC	5 cut(s)	661, 813, 825, 840, 1132
ApeKI	GCWGC	4 cut(s)	66, 793, 809, 946
ArsI	GACNNNNNNTTYG	2 cut(s)	405, 437
AsiGI	ACCGGT	1 cut(s)	998
Asp700I	GAANNNNTTC	1 cut(s)	115
AspLEI	GCGC	2 cut(s)	195, 881
AspS9I	GGNCC	1 cut(s)	639
AsuC2I	CCSGG	1 cut(s)	737
AsuHPI	GGTGA	9 cut(s)	15, 73, 397, 601, 793, 811, 928, 949, 1187
AvaII	GGWCC	1 cut(s)	639
BalI	TGGCCA	1 cut(s)	827
BbrPI	CACGTG	1 cut(s)	671
BbvI	GCAGC	4 cut(s)	53, 796, 805, 958
BccI	CCATC	6 cut(s)	796, 832, 1018, 1101, 1181, 1216
BceAI	ACGGC	2 cut(s)	1032, 1147
BclI	TGATCA	4 cut(s)	58, 132, 177, 213
BcnI	CCSGG	1 cut(s)	737
BcoDI	GTCTC	3 cut(s)	250, 502, 1185
BfaI	CTAG	1 cut(s)	1149
BfoI	RGCGCY	1 cut(s)	196
BisI	GCNGC	5 cut(s)	67, 794, 810, 813, 947
BlsI	GCNGC	5 cut(s)	68, 795, 811, 814, 948
BmcAI	AGTACT	1 cut(s)	269
Bme1390I	CCNGG	1 cut(s)	737
Bme18I	GGWCC	1 cut(s)	639
BmgBI	CACGTC	1 cut(s)	942
BmgT120I	GGNCC	1 cut(s)	639
BmiI	GGNNCC	2 cut(s)	640, 1204
BmrFI	CCNGG	1 cut(s)	737
Bpu10I	CCTNAGC	1 cut(s)	614
BpuEI	CTTGAG	1 cut(s)	274
BpuMI	CCSGG	1 cut(s)	737
BsaAI	YACGTR	1 cut(s)	671
BsaJI	CCNNGG	3 cut(s)	777, 816, 828
BsaWI	WCCGGW	1 cut(s)	998
BsaXI	ACNNNNNCTCC	4 cut(s)	179, 209, 891, 921
Bsc4I	CCNNNNNNNGG	2 cut(s)	742, 834
Bse118I	RCCGGY	2 cut(s)	968, 998
Bse1I	ACTGG	2 cut(s)	494, 633
Bse3DI	GCAATG	1 cut(s)	373
BseDI	CCNNGG	3 cut(s)	777, 816, 828
BseGI	GGATG	7 cut(s)	96, 276, 543, 843, 1035, 1112, 1231
BseLI	CCNNNNNNNGG	2 cut(s)	742, 834
BseMI	GCAATG	1 cut(s)	373
BseMII	CTCAG	2 cut(s)	149, 633
BseNI	ACTGG	2 cut(s)	494, 633
BseRI	GAGGAG	8 cut(s)	326, 359, 496, 503, 506, 511, 596, 1197
BseXI	GCAGC	4 cut(s)	53, 796, 805, 958
BseYI	CCCAGC	1 cut(s)	448
Bsh1236I	CGCG	2 cut(s)	861, 881
Bsh1285I	CGRYCG	1 cut(s)	834
BshFI	GGCC	5 cut(s)	663, 815, 827, 842, 1134
BshTI	ACCGGT	1 cut(s)	998
BsiEI	CGRYCG	1 cut(s)	834
BsiSI	CCGG	3 cut(s)	737, 969, 999
BslFI	GGGAC	1 cut(s)	976
BslI	CCNNNNNNNGG	2 cut(s)	742, 834
BsmAI	GTCTC	3 cut(s)	250, 502, 1185
BsmFI	GGGAC	1 cut(s)	976
BsnI	GGCC	5 cut(s)	663, 815, 827, 842, 1134
Bsp143I	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
Bsp19I	CCATGG	1 cut(s)	777
Bsp68I	TCGCGA	1 cut(s)	861
BspACI	CCGC	3 cut(s)	812, 852, 1170
BspANI	GGCC	5 cut(s)	663, 815, 827, 842, 1134
BspCNI	CTCAG	2 cut(s)	150, 632
BspFNI	CGCG	2 cut(s)	861, 881
BspLI	GGNNCC	2 cut(s)	640, 1204
BspPI	GGATC	1 cut(s)	247
BsrDI	GCAATG	1 cut(s)	373
BsrFI	RCCGGY	2 cut(s)	968, 998
BsrI	ACTGG	2 cut(s)	494, 633
BssAI	RCCGGY	2 cut(s)	968, 998
BssECI	CCNNGG	3 cut(s)	777, 816, 828
BssMI	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
BssNAI	GTATAC	1 cut(s)	1129
BssT1I	CCWWGG	1 cut(s)	777
Bst1107I	GTATAC	1 cut(s)	1129
Bst4CI	ACNGT	3 cut(s)	820, 832, 922
Bst6I	CTCTTC	4 cut(s)	236, 253, 473, 994
BstBAI	YACGTR	1 cut(s)	671
BstC8I	GCNNGC	4 cut(s)	257, 712, 716, 970
BstDEI	CTNAG	5 cut(s)	158, 418, 614, 619, 685
BstDSI	CCRYGG	3 cut(s)	777, 816, 828
BstEII	GGTNACC	1 cut(s)	916
BstF5I	GGATG	7 cut(s)	96, 276, 543, 843, 1035, 1112, 1231
BstFNI	CGCG	2 cut(s)	861, 881
BstH2I	RGCGCY	1 cut(s)	196
BstHHI	GCGC	2 cut(s)	195, 881
BstKTI	GATC	6 cut(s)	61, 135, 180, 216, 242, 786
BstMAI	GTCTC	3 cut(s)	250, 502, 1185
BstMBI	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
BstMCI	CGRYCG	1 cut(s)	834
BstMWI	GCNNNNNNNGC	3 cut(s)	168, 199, 858
BstPI	GGTNACC	1 cut(s)	916
BstSCI	CCNGG	1 cut(s)	735
BstUI	CGCG	2 cut(s)	861, 881
BstV1I	GCAGC	4 cut(s)	53, 796, 805, 958
BstXI	CCANNNNNNTGG	2 cut(s)	62, 823
BstZ17I	GTATAC	1 cut(s)	1129
BsuRI	GGCC	5 cut(s)	663, 815, 827, 842, 1134
BtgI	CCRYGG	3 cut(s)	777, 816, 828
BtrI	CACGTC	1 cut(s)	942
BtsCI	GGATG	7 cut(s)	96, 276, 543, 843, 1035, 1112, 1231
BtuMI	TCGCGA	1 cut(s)	861
Cac8I	GCNNGC	4 cut(s)	257, 712, 716, 970
CfoI	GCGC	2 cut(s)	195, 881
Cfr10I	RCCGGY	2 cut(s)	968, 998
Cfr13I	GGNCC	1 cut(s)	639
Csp6I	GTAC	2 cut(s)	268, 1057
CspAI	ACCGGT	1 cut(s)	998
CviQI	GTAC	2 cut(s)	268, 1057
DdeI	CTNAG	5 cut(s)	158, 418, 614, 619, 685
DpnI	GATC	6 cut(s)	60, 134, 179, 215, 241, 785
DpnII	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
EaeI	YGGCCR	2 cut(s)	813, 825
Eam1104I	CTCTTC	4 cut(s)	236, 253, 473, 994
EarI	CTCTTC	4 cut(s)	236, 253, 473, 994
Eco130I	CCWWGG	1 cut(s)	777
Eco47I	GGWCC	1 cut(s)	639
Eco47III	AGCGCT	1 cut(s)	194
Eco72I	CACGTG	1 cut(s)	671
Eco91I	GGTNACC	1 cut(s)	916
EcoO65I	GGTNACC	1 cut(s)	916
EcoT14I	CCWWGG	1 cut(s)	777
ErhI	CCWWGG	1 cut(s)	777
FalI	AAGNNNNNCTT	2 cut(s)	668, 700
FaqI	GGGAC	1 cut(s)	976
FauI	CCCGC	1 cut(s)	845
FbaI	TGATCA	4 cut(s)	58, 132, 177, 213
FblI	GTMKAC	1 cut(s)	1128
Fnu4HI	GCNGC	5 cut(s)	67, 794, 810, 813, 947
FokI	GGATG	7 cut(s)	83, 263, 530, 850, 1042, 1119, 1238
Fsp4HI	GCNGC	5 cut(s)	67, 794, 810, 813, 947
FspBI	CTAG	1 cut(s)	1149
GlaI	GCGC	2 cut(s)	194, 880
GluI	GCNGC	5 cut(s)	67, 794, 810, 813, 947
GsaI	CCCAGC	1 cut(s)	452
HaeII	RGCGCY	1 cut(s)	196
HaeIII	GGCC	5 cut(s)	663, 815, 827, 842, 1134
HapII	CCGG	3 cut(s)	737, 969, 999
HhaI	GCGC	2 cut(s)	195, 881
Hin6I	GCGC	2 cut(s)	193, 879
HinP1I	GCGC	2 cut(s)	193, 879
HincII	GTYRAC	2 cut(s)	306, 996
HindII	GTYRAC	2 cut(s)	306, 996
HinfI	GANTC	1 cut(s)	1064
HpaII	CCGG	3 cut(s)	737, 969, 999
HphI	GGTGA	9 cut(s)	15, 73, 397, 601, 793, 811, 928, 949, 1187
Hpy166II	GTNNAC	5 cut(s)	306, 766, 996, 1057, 1129
Hpy188III	TCNNGA	3 cut(s)	130, 835, 860
Hpy8I	GTNNAC	5 cut(s)	306, 766, 996, 1057, 1129
HpyAV	CCTTC	7 cut(s)	350, 403, 862, 1075, 1160, 1172, 1193
HpyCH4III	ACNGT	3 cut(s)	820, 832, 922
HpyCH4IV	ACGT	5 cut(s)	308, 670, 906, 933, 941
HpyCH4V	TGCA	5 cut(s)	378, 793, 930, 1011, 1231
HpyF10VI	GCNNNNNNNGC	3 cut(s)	168, 199, 858
HpyF3I	CTNAG	5 cut(s)	158, 418, 614, 619, 685
HpySE526I	ACGT	5 cut(s)	308, 670, 906, 933, 941
HspAI	GCGC	2 cut(s)	193, 879
KroI	GCCGGC	1 cut(s)	968
KroNI	GCCGGC	1 cut(s)	970
Ksp22I	TGATCA	4 cut(s)	58, 132, 177, 213
Kzo9I	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
LmnI	GCTCC	1 cut(s)	200
Lsp1109I	GCAGC	4 cut(s)	53, 796, 805, 958
MaeI	CTAG	1 cut(s)	1149
MaeII	ACGT	5 cut(s)	308, 670, 906, 933, 941
MaeIII	GTNAC	4 cut(s)	479, 574, 916, 937
MalI	GATC	6 cut(s)	60, 134, 179, 215, 241, 785
MboI	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
MboII	GAAGA	7 cut(s)	223, 240, 460, 685, 760, 981, 1175
MfeI	CAATTG	2 cut(s)	592, 702
MlsI	TGGCCA	1 cut(s)	827
MluCI	AATT	6 cut(s)	115, 217, 429, 555, 592, 702
MluNI	TGGCCA	1 cut(s)	827
MmeI	TCCRAC	1 cut(s)	666
Mox20I	TGGCCA	1 cut(s)	827
MroNI	GCCGGC	1 cut(s)	968
MroXI	GAANNNNTTC	1 cut(s)	115
MscI	TGGCCA	1 cut(s)	827
MseI	TTAA	2 cut(s)	983, 1255
MslI	CAYNNNNRTG	2 cut(s)	60, 299
Msp20I	TGGCCA	1 cut(s)	827
MspA1I	CMGCKG	1 cut(s)	796
MspI	CCGG	3 cut(s)	737, 969, 999
MspR9I	CCNGG	1 cut(s)	737
MunI	CAATTG	2 cut(s)	592, 702
MvnI	CGCG	2 cut(s)	861, 881
MwoI	GCNNNNNNNGC	3 cut(s)	168, 199, 858
NaeI	GCCGGC	1 cut(s)	970
NciI	CCSGG	1 cut(s)	737
NcoI	CCATGG	1 cut(s)	777
NdeII	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
NgoMIV	GCCGGC	1 cut(s)	968
NlaIV	GGNNCC	2 cut(s)	640, 1204
NmuCI	GTSAC	2 cut(s)	916, 937
NruI	TCGCGA	1 cut(s)	861
PdiI	GCCGGC	1 cut(s)	970
PdmI	GAANNNNTTC	1 cut(s)	115
PfeI	GAWTC	1 cut(s)	1064
PinAI	ACCGGT	1 cut(s)	998
PkrI	GCNGC	5 cut(s)	68, 795, 811, 814, 948
PmaCI	CACGTG	1 cut(s)	671
PmlI	CACGTG	1 cut(s)	671
Ppu21I	YACGTR	1 cut(s)	671
PspCI	CACGTG	1 cut(s)	671
PspEI	GGTNACC	1 cut(s)	916
PspFI	CCCAGC	1 cut(s)	448
PspN4I	GGNNCC	2 cut(s)	640, 1204
PspPI	GGNCC	1 cut(s)	639
PvuII	CAGCTG	1 cut(s)	796
RruI	TCGCGA	1 cut(s)	861
RsaI	GTAC	2 cut(s)	269, 1058
RsaNI	GTAC	2 cut(s)	268, 1057
RseI	CAYNNNNRTG	2 cut(s)	60, 299
SaqAI	TTAA	2 cut(s)	983, 1255
SatI	GCNGC	5 cut(s)	67, 794, 810, 813, 947
Sau3AI	GATC	6 cut(s)	58, 132, 177, 213, 239, 783
Sau96I	GGNCC	1 cut(s)	639
ScaI	AGTACT	1 cut(s)	269
ScrFI	CCNGG	1 cut(s)	737
SinI	GGWCC	1 cut(s)	639
SmiMI	CAYNNNNRTG	2 cut(s)	60, 299
SmlI	CTYRAG	1 cut(s)	289
SmoI	CTYRAG	1 cut(s)	289
Sse9I	AATT	6 cut(s)	115, 217, 429, 555, 592, 702
SsiI	CCGC	3 cut(s)	812, 852, 1170
SspMI	CTAG	1 cut(s)	1149
StyD4I	CCNGG	1 cut(s)	735
StyI	CCWWGG	1 cut(s)	777
TaaI	ACNGT	3 cut(s)	820, 832, 922
TaiI	ACGT	5 cut(s)	311, 673, 909, 936, 944
TaqI	TCGA	1 cut(s)	786
TasI	AATT	6 cut(s)	115, 217, 429, 555, 592, 702
TatI	WGTACW	1 cut(s)	267
TauI	GCSGC	1 cut(s)	815
TfiI	GAWTC	1 cut(s)	1064
Tru1I	TTAA	2 cut(s)	983, 1255
Tru9I	TTAA	2 cut(s)	983, 1255
TseFI	GTSAC	2 cut(s)	916, 937
TseI	GCWGC	4 cut(s)	66, 793, 809, 946
Tsp45I	GTSAC	2 cut(s)	916, 937
TspDTI	ATGAA	8 cut(s)	44, 108, 223, 265, 343, 881, 946, 1046
VpaK11BI	GGWCC	1 cut(s)	639
XcmI	CCANNNNNNNNNTGG	2 cut(s)	59, 835
XmiI	GTMKAC	1 cut(s)	1128
XmnI	GAANNNNTTC	1 cut(s)	115
XspI	CTAG	1 cut(s)	1149
ZrmI	AGTACT	1 cut(s)	269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.