Rh5BG158000

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015201
Physical Location & Seq
Reverse (-)
16125414 .. 16127074
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG158000.1

Sequence Viewer

Length: 1095 bp
ATGCCCCCATCCGCCGATATGACCCCCCACACTCCCAAAAACAAAGGCTCTCTCCCCTTGTTGGTGATGAACTACCTCCTCCTCTTTGTTGGCTCTGTCTCAGCCAGTCTCCTCTCCAAATACTATTTCATTCATAAAGGCTCAAGCATTTGGGTCGCTACATGGGTTCAGTGTGCTGGCTTTCCTCTTCTTCTCCCCCCAATTATCCTCCCTTATTTCCTCAACCTCACCACCAGAAGACCCTTCACCCAATTCACCTCCAAAATCTTCTCCATCTCCATCTTCGTGGGCCTTATGTTGGGCCTCAACAACCTCCTCTTCTCCGTCGGCAACTCCAACCTCCCGGTCTCCACCTCTTCCCTCCTTCTCTCCTCCCAACTCGTCTTCAACCTCCTCCTCTCCGTACTCATCGTCAAACAGAAGGTCACATTCTCCAACCTTAACTGCGTCATCCTCCTAACTGTCAGCTCCATCCTCTTAGCCCTTGGCTCGAGCCACGACAAGCCTCCCGGTCTGACACGTGGAAAGTACTTCGTCGGATTCTTCTCCACCATCGGCGCCGGCTTGCTCTTCGCTCTCTACCTCCCCATCATGGAAAAAGTCTACAGAAAAGTCGACTGCTACTCCATGATGATGGAAATGCAGGCGGTGATGGAGGCCGCCGCGACGGTGTTGTCGACGGCGTTGATGGTGTGGAACGGTGCGTTTGGTGGCATGGTGAGGGAGGGGAGGGAGCTGTTTGACAAGGGAGAGAGGGTATATTGGGTGACGGTGGCGTTTAACGTCGTGACGTGGCAGCTGTGCTTCATGGGGACGGCGGGGATGGTTTTCTTGACGACGTCGCTGACCGGCGGGATATGCATGACGGCGTTGATGGGGATGAACGTTTTGGGGGGAGTGGTGGTTTACGGGGACAAGTTTGGTGGGGTGAAAGCGGTTTCGACTGTGCTTTGTGTATGGGGGTTCTGTTCTTATGTGTACGGGATTTACATGAAGTCGAAGAAGATTAGGGAGGACGACGACGACGAGGACCGCACCGAGATGATAGAGATGGGTAGAAATCCGAGTGTACGTAACGGTGAAATTCATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

364

Amino Acids

40.06

Weight (kDa)

8.98

Isoelectric Point (pI)

21.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 20 - 335 2.6e-94 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015201)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30840 AT1G30840
fragaria_vesca FvH4_3g13350 FvH4_6g07670
malus_domestica MD05G1242500.v1.1 MD10G1224300.v1.1
prunus_persica Prupe.4G120900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0022031
rosa_laevigata RLG00000032639
rosa_multiflora Rmu_sc0001585.1_g000011
rosa_rugosa Rorug05G0070800
rosa_samantha Rh5AG158700 Rh5BG158000 Rh5CG172500 Rh5DG158200
rosa_wichuraiana Rw5G014140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 673
AatII GACGTC 1 cut(s) 842
AccB1I GGYRCC 1 cut(s) 557
AccI GTMKAC 3 cut(s) 603, 615, 677
AccII CGCG 1 cut(s) 665
AciI CCGC 8 cut(s) 12, 647, 660, 663, 818, 852, 935, 1033
AclI AACGTT 1 cut(s) 885
AcsI RAATTY 1 cut(s) 1083
AcvI CACGTG 1 cut(s) 521
AcyI GRCGYC 2 cut(s) 558, 839
AfaI GTAC 4 cut(s) 405, 530, 980, 1071
AfiI CCNNNNNNNGG 3 cut(s) 61, 298, 592
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 1 cut(s) 388
AjiI CACGTC 1 cut(s) 792
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 3 cut(s) 468, 736, 799
AluI AGCT 3 cut(s) 468, 736, 799
Alw26I GTCTC 3 cut(s) 103, 113, 352
Ama87I CYCGRG 1 cut(s) 490
AoxI GGCC 3 cut(s) 289, 301, 657
ApeKI GCWGC 1 cut(s) 796
ApoI RAATTY 1 cut(s) 1083
ArsI GACNNNNNNTTYG 2 cut(s) 408, 440
AspLEI GCGC 1 cut(s) 560
AspS9I GGNCC 3 cut(s) 289, 301, 1030
AsuC2I CCSGG 2 cut(s) 344, 510
AsuHPI GGTGA 9 cut(s) 76, 220, 238, 247, 661, 730, 778, 940, 1091
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 1 cut(s) 1030
BanI GGYRCC 1 cut(s) 557
BbrPI CACGTG 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 244, 376
BbvI GCAGC 1 cut(s) 808
BceAI ACGGC 3 cut(s) 696, 831, 882
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BcnI CCSGG 2 cut(s) 344, 510
BcoDI GTCTC 3 cut(s) 103, 113, 352
BfmI CTRYAG 1 cut(s) 604
BfoI RGCGCY 1 cut(s) 561
BisI GCNGC 3 cut(s) 660, 663, 797
BlsI GCNGC 3 cut(s) 661, 664, 798
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 344, 510
Bme18I GGWCC 1 cut(s) 1030
BmeT110I CYCGRG 1 cut(s) 490
BmgBI CACGTC 1 cut(s) 792
BmgT120I GGNCC 3 cut(s) 289, 301, 1030
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 2 cut(s) 344, 510
BpiI GAAGAC 2 cut(s) 244, 376
BpuEI CTTGAG 1 cut(s) 127
BpuMI CCSGG 2 cut(s) 344, 510
BsaAI YACGTR 2 cut(s) 521, 1073
BsaHI GRCGYC 2 cut(s) 558, 839
BsaI GGTCTC 1 cut(s) 352
BsaJI CCNNGG 1 cut(s) 484
BsaXI ACNNNNNCTCC 6 cut(s) 608, 638, 741, 771, 888, 918
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 298, 592
Bse118I RCCGGY 2 cut(s) 560, 848
Bse1I ACTGG 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 484
BseGI GGATG 5 cut(s) 8, 450, 471, 828, 885
BseLI CCNNNNNNNGG 3 cut(s) 61, 298, 592
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 7 cut(s) 68, 71, 101, 305, 361, 383, 386
BseXI GCAGC 1 cut(s) 808
Bsh1236I CGCG 1 cut(s) 665
BshFI GGCC 3 cut(s) 291, 303, 659
BshNI GGYRCC 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 4 cut(s) 344, 510, 561, 849
BslFI GGGAC 2 cut(s) 826, 926
BslI CCNNNNNNNGG 3 cut(s) 61, 298, 592
BsmAI GTCTC 3 cut(s) 103, 113, 352
BsmFI GGGAC 2 cut(s) 826, 926
BsnI GGCC 3 cut(s) 291, 303, 659
Bso31I GGTCTC 1 cut(s) 352
BsoBI CYCGRG 1 cut(s) 490
BspACI CCGC 8 cut(s) 12, 647, 660, 663, 818, 852, 935, 1033
BspANI GGCC 3 cut(s) 291, 303, 659
BspCNI CTCAG 1 cut(s) 113
BspFNI CGCG 1 cut(s) 665
BspLI GGNNCC 1 cut(s) 559
BspQI GCTCTTC 1 cut(s) 575
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 352
BsrFI RCCGGY 2 cut(s) 560, 848
BsrI ACTGG 1 cut(s) 105
BssAI RCCGGY 2 cut(s) 560, 848
BssECI CCNNGG 1 cut(s) 484
BssNI GRCGYC 2 cut(s) 558, 839
BssT1I CCWWGG 1 cut(s) 484
Bst4CI ACNGT 6 cut(s) 463, 670, 701, 772, 946, 1079
Bst6I CTCTTC 4 cut(s) 192, 323, 361, 575
BstACI GRCGYC 2 cut(s) 558, 839
BstBAI YACGTR 2 cut(s) 521, 1073
BstC8I GCNNGC 4 cut(s) 178, 562, 566, 645
BstDEI CTNAG 2 cut(s) 100, 478
BstF5I GGATG 5 cut(s) 8, 450, 471, 828, 885
BstFNI CGCG 1 cut(s) 665
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 1 cut(s) 560
BstMAI GTCTC 3 cut(s) 103, 113, 352
BstMWI GCNNNNNNNGC 1 cut(s) 858
BstSCI CCNGG 2 cut(s) 342, 508
BstSFI CTRYAG 1 cut(s) 604
BstSNI TACGTA 1 cut(s) 1073
BstUI CGCG 1 cut(s) 665
BstV1I GCAGC 1 cut(s) 808
BstV2I GAAGAC 2 cut(s) 244, 376
BstXI CCANNNNNNTGG 2 cut(s) 286, 634
BsuRI GGCC 3 cut(s) 291, 303, 659
BtrI CACGTC 1 cut(s) 792
BtsCI GGATG 5 cut(s) 8, 450, 471, 828, 885
BtsIMutI CAGTG 1 cut(s) 176
Cac8I GCNNGC 4 cut(s) 178, 562, 566, 645
CfoI GCGC 1 cut(s) 560
Cfr10I RCCGGY 2 cut(s) 560, 848
Cfr13I GGNCC 3 cut(s) 289, 301, 1030
CseI GACGC 1 cut(s) 436
Csp6I GTAC 4 cut(s) 404, 529, 979, 1070
CspCI CAANNNNNGTGG 2 cut(s) 904, 939
CviAII CATG 8 cut(s) 162, 592, 628, 715, 808, 862, 991, 1088
CviQI GTAC 4 cut(s) 404, 529, 979, 1070
DdeI CTNAG 2 cut(s) 100, 478
DinI GGCGCC 1 cut(s) 559
DrdI GACNNNNNNGTC 1 cut(s) 673
DseDI GACNNNNNNGTC 1 cut(s) 673
Eam1104I CTCTTC 4 cut(s) 192, 323, 361, 575
EarI CTCTTC 4 cut(s) 192, 323, 361, 575
Eco105I TACGTA 1 cut(s) 1073
Eco130I CCWWGG 1 cut(s) 484
Eco31I GGTCTC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 1030
Eco72I CACGTG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 1 cut(s) 863
EgeI GGCGCC 1 cut(s) 559
EheI GGCGCC 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 8 cut(s) 165, 595, 631, 718, 811, 865, 994, 1091
FaqI GGGAC 2 cut(s) 826, 926
FatI CATG 8 cut(s) 161, 591, 627, 714, 807, 861, 990, 1087
FauI CCCGC 2 cut(s) 811, 845
FblI GTMKAC 3 cut(s) 603, 615, 677
Fnu4HI GCNGC 3 cut(s) 660, 663, 797
FokI GGATG 4 cut(s) 437, 458, 835, 892
Fsp4HI GCNGC 3 cut(s) 660, 663, 797
GlaI GCGC 1 cut(s) 559
GluI GCNGC 3 cut(s) 660, 663, 797
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 3 cut(s) 291, 303, 659
HapII CCGG 4 cut(s) 344, 510, 561, 849
HgaI GACGC 1 cut(s) 436
HhaI GCGC 1 cut(s) 560
Hin1I GRCGYC 2 cut(s) 558, 839
Hin1II CATG 8 cut(s) 165, 595, 631, 718, 811, 865, 994, 1091
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HincII GTYRAC 2 cut(s) 616, 678
HindII GTYRAC 2 cut(s) 616, 678
HinfI GANTC 1 cut(s) 540
HpaII CCGG 4 cut(s) 344, 510, 561, 849
HphI GGTGA 9 cut(s) 76, 220, 238, 247, 661, 730, 778, 940, 1091
Hpy166II GTNNAC 6 cut(s) 604, 616, 678, 907, 979, 1070
Hpy188I TCNGA 3 cut(s) 516, 539, 1065
Hpy188III TCNNGA 2 cut(s) 787, 832
Hpy8I GTNNAC 6 cut(s) 604, 616, 678, 907, 979, 1070
HpyAV CCTTC 3 cut(s) 253, 374, 415
HpyCH4III ACNGT 6 cut(s) 463, 670, 701, 772, 946, 1079
HpyCH4IV ACGT 6 cut(s) 520, 783, 791, 839, 885, 1072
HpyCH4V TGCA 2 cut(s) 643, 861
HpyF10VI GCNNNNNNNGC 1 cut(s) 858
HpyF3I CTNAG 2 cut(s) 100, 478
HpySE526I ACGT 6 cut(s) 520, 783, 791, 839, 885, 1072
Hsp92I GRCGYC 2 cut(s) 558, 839
Hsp92II CATG 8 cut(s) 165, 595, 631, 718, 811, 865, 994, 1091
HspAI GCGC 1 cut(s) 558
KasI GGCGCC 1 cut(s) 557
KroI GCCGGC 1 cut(s) 560
KroNI GCCGGC 1 cut(s) 562
LguI GCTCTTC 1 cut(s) 575
LmnI GCTCC 2 cut(s) 473, 733
LpnPI CCDG 8 cut(s) 118, 162, 247, 357, 523, 574, 629, 862
Lsp1109I GCAGC 1 cut(s) 808
MaeII ACGT 6 cut(s) 520, 783, 791, 839, 885, 1072
MaeIII GTNAC 4 cut(s) 424, 766, 787, 1073
MluCI AATT 3 cut(s) 201, 251, 1083
Mly113I GGCGCC 1 cut(s) 558
MmeI TCCRAC 3 cut(s) 360, 459, 517
Mph1103I ATGCAT 1 cut(s) 863
MroNI GCCGGC 1 cut(s) 560
MseI TTAA 2 cut(s) 441, 780
MslI CAYNNNNRTG 3 cut(s) 284, 632, 1040
MspA1I CMGCKG 1 cut(s) 799
MspI CCGG 4 cut(s) 344, 510, 561, 849
MspR9I CCNGG 2 cut(s) 344, 510
MvnI CGCG 1 cut(s) 665
MwoI GCNNNNNNNGC 1 cut(s) 858
NaeI GCCGGC 1 cut(s) 562
NarI GGCGCC 1 cut(s) 558
NciI CCSGG 2 cut(s) 344, 510
NgoMIV GCCGGC 1 cut(s) 560
NlaIII CATG 8 cut(s) 165, 595, 631, 718, 811, 865, 994, 1091
NlaIV GGNNCC 1 cut(s) 559
NmuCI GTSAC 3 cut(s) 424, 766, 787
NsiI ATGCAT 1 cut(s) 863
PaeR7I CTCGAG 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 575
PcsI WCGNNNNNNNCGW 2 cut(s) 674, 1023
PdiI GCCGGC 1 cut(s) 562
PfeI GAWTC 1 cut(s) 540
PflFI GACNNNGTC 1 cut(s) 838
PkrI GCNGC 3 cut(s) 661, 664, 798
PluTI GGCGCC 1 cut(s) 561
PmaCI CACGTG 1 cut(s) 521
PmlI CACGTG 1 cut(s) 521
Ppu21I YACGTR 2 cut(s) 521, 1073
Psp1406I AACGTT 1 cut(s) 885
PspCI CACGTG 1 cut(s) 521
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 3 cut(s) 289, 301, 1030
PspXI VCTCGAGB 1 cut(s) 490
PsyI GACNNNGTC 1 cut(s) 838
PvuII CAGCTG 1 cut(s) 799
RsaI GTAC 4 cut(s) 405, 530, 980, 1071
RsaNI GTAC 4 cut(s) 404, 529, 979, 1070
RseI CAYNNNNRTG 3 cut(s) 284, 632, 1040
SalI GTCGAC 2 cut(s) 614, 676
SapI GCTCTTC 1 cut(s) 575
SaqAI TTAA 2 cut(s) 441, 780
SatI GCNGC 3 cut(s) 660, 663, 797
Sau96I GGNCC 3 cut(s) 289, 301, 1030
ScaI AGTACT 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 344, 510
SfcI CTRYAG 1 cut(s) 604
SfoI GGCGCC 1 cut(s) 559
Sfr274I CTCGAG 1 cut(s) 490
SinI GGWCC 1 cut(s) 1030
SlaI CTCGAG 1 cut(s) 490
SmiMI CAYNNNNRTG 3 cut(s) 284, 632, 1040
SmlI CTYRAG 2 cut(s) 142, 490
SmoI CTYRAG 2 cut(s) 142, 490
SnaBI TACGTA 1 cut(s) 1073
Sse9I AATT 3 cut(s) 201, 251, 1083
SsiI CCGC 8 cut(s) 12, 647, 660, 663, 818, 852, 935, 1033
SspDI GGCGCC 1 cut(s) 557
StyD4I CCNGG 2 cut(s) 342, 508
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 6 cut(s) 463, 670, 701, 772, 946, 1079
TaiI ACGT 6 cut(s) 523, 786, 794, 842, 888, 1075
TaqI TCGA 5 cut(s) 491, 615, 677, 941, 998
TasI AATT 3 cut(s) 201, 251, 1083
TatI WGTACW 1 cut(s) 528
TauI GCSGC 2 cut(s) 662, 665
TfiI GAWTC 1 cut(s) 540
Tru1I TTAA 2 cut(s) 441, 780
Tru9I TTAA 2 cut(s) 441, 780
TscAI CASTG 1 cut(s) 176
TseFI GTSAC 3 cut(s) 424, 766, 787
TseI GCWGC 1 cut(s) 796
Tsp45I GTSAC 3 cut(s) 424, 766, 787
TspDTI ATGAA 7 cut(s) 83, 118, 122, 796, 896, 1007, 1076
TspGWI ACGGA 2 cut(s) 313, 391
TspRI CASTG 1 cut(s) 176
Tth111I GACNNNGTC 1 cut(s) 838
VpaK11BI GGWCC 1 cut(s) 1030
XapI RAATTY 1 cut(s) 1083
XhoI CTCGAG 1 cut(s) 490
XmiI GTMKAC 3 cut(s) 603, 615, 677
ZraI GACGTC 1 cut(s) 840
ZrmI AGTACT 1 cut(s) 530
Zsp2I ATGCAT 1 cut(s) 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.