AT1G32060

Phosphoribulokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014052
Physical Location & Seq
Forward (+)
11532199 .. 11534639
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32060.1

Sequence Viewer

Length: 1188 bp
ATGGCTGTCTCAACTATCTACTCAACACAAGCTCTCAATTCAACTCATTTCTTAACCTCTTCTTCCTCCTCCAAACAAGTCTTCCTCTACCGTCGTCAACCACAAACCAACCGTAGATTCAACACACTCATCACTTGCGCACAAGAAACCATCGTGATCGGACTAGCTGCTGACTCTGGCTGCGGCAAAAGTACCTTTATGCGGAGGCTCACCAGCGTCTTTGGTGGCGCTGCTAAGCCACCAAAAGGCGGGAACCCTGATTCCAACACACTCATCAGCGACACGACCACTGTGATCTGTCTTGATGATTACCATTCTTTGGATAGGTACGGTAGGAAAGAGCAGAAAGTCACCGCTTTGGACCCACGCGCCAATGACTTTGATCTCATGTATGAGCAAGTCAAAGCTCTTAAGAATGGTATAGCCGTCGAGAAACCGATTTATAACCATGTCACTGGACTTCTTGACCCTCCGGAGCTTATTCAGCCTCCTAAGATTCTTGTCATCGAAGGTCTTCACCCAATGTTTGATGAGCGAGTAAGAGACTTACTAGACTTCAGTATCTACTTGGACATTAGCAACGAAGTCAAATTCGCTTGGAAAATTCAGAGGGACATGGCTGAAAGAGGTCACAGTTTGGAGAGCATCAAAGCGAGTATCGAAGCCCGAAAGCCCGACTTCGATGCATTCATCGACCCGCAAAAGCAGTACGCGGATGCGGTCATAGAAGTGCTTCCTACGACTCTGATCCCAGATGACAACGAAGGGAAAGTGTTGAGAGTGAGATTGATAATGAAGGAAGGTGTTAAGTACTTCAGCCCGGTTTACCTGTTCGATGAAGGTTCAACCATCTCGTGGATTCCTTGCGGCCGCAAACTCACTTGCTCGTACCCTGGCATCAAGTTCAACTACGAACCTGACTCCTACTTCGACCATGAGGTATCAGTTTTGGAGATGGATGGACAATTTGATAGACTGGACGAGCTGATTTACGTGGAAAGTCACTTGAGCAACCTCTCGACCAAATTCTACGGAGAAGTCACTCAACAAATGCTCAAACATGCTGATTTCCCGGGTAGCAACAACGGTACTGGTCTTTTCCAAACCATTGTTGGATTGAAGATCAGAGATCTCTATGAGCAGCTCATTGCCAACAAAGCCACTGCTCGTGCAGAAGCTAAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004849 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006206 GO:0006213 GO:0006220 GO:0006221 GO:0006222 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008655 GO:0008974 GO:0009058 GO:0009112 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009173 GO:0009174 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0010319 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0019904 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042455 GO:0042651 GO:0042742 GO:0042802 GO:0042803 GO:0043094 GO:0043097 GO:0043174 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046049 GO:0046131 GO:0046132 GO:0046134 GO:0046390 GO:0046483 GO:0046983 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055035 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:0097718 GO:0098542 GO:0099080 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659
KEGG Pathways
Metabolic & Signaling
ko00710 ko01100 ko01120 ko01200 map00710 map01100 map01120 map01200
Pfam Domains
Protein Families
PRK

Protein Analysis

395

Amino Acids

44.46

Weight (kDa)

5.71

Isoelectric Point (pI)

25.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRK PF00485 52 - 250 9.4e-81 Phosphoribulokinase / Uridine kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32060
fragaria_vesca FvH4_5g23520
malus_domestica MD00G1068500.v1.1 MD06G1023500.v1.1
prunus_persica Prupe.5G024600_v2.0.a1
pyrus_communis pycom06g01910 pycom16g25320
rosa_chinensis RchiOBHm_Chr7g0212791
rosa_laevigata RLG00000002868
rosa_multiflora Rmu_sc0000394.1_g000036
rosa_roxburghii Rroxscaffold_3G00246550
rosa_rugosa Rorug07G0136800
rosa_samantha Rh7AG268300 Rh7BG263700 Rh7CG287300
rosa_wichuraiana Rw7G023180 Rw7G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 444
Acc16I TGCGCA 1 cut(s) 139
AccB7I CCANNNNNTGG 2 cut(s) 319, 855
AccII CGCG 2 cut(s) 369, 713
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 9 cut(s) 183, 202, 249, 354, 698, 713, 719, 867, 871
AclWI GGATC 1 cut(s) 742
AcoI YGGCCR 1 cut(s) 868
AcsI RAATTY 3 cut(s) 590, 603, 1025
AcuI CTGAAG 2 cut(s) 541, 799
AfaI GTAC 6 cut(s) 193, 329, 710, 812, 890, 1090
AfiI CCNNNNNNNGG 5 cut(s) 201, 245, 248, 319, 855
AflII CTTAAG 1 cut(s) 410
AgsI TTSAA 5 cut(s) 42, 121, 846, 907, 1120
AjnI CCWGG 1 cut(s) 892
AjuI GAANNNNNNNTTGG 4 cut(s) 65, 97, 101, 133
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
AluBI AGCT 7 cut(s) 32, 167, 407, 478, 985, 1144, 1178
AluI AGCT 7 cut(s) 32, 167, 407, 478, 985, 1144, 1178
Alw26I GTCTC 2 cut(s) 13, 537
AlwI GGATC 1 cut(s) 742
Ama87I CYCGRG 1 cut(s) 1072
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 1 cut(s) 868
ApeKI GCWGC 4 cut(s) 167, 180, 230, 1141
ApoI RAATTY 3 cut(s) 590, 603, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 911, 943
Asp700I GAANNNNTTC 2 cut(s) 513, 732
AspLEI GCGC 3 cut(s) 140, 230, 371
AspS9I GGNCC 1 cut(s) 361
AsuC2I CCSGG 3 cut(s) 821, 1073, 1074
AsuHPI GGTGA 3 cut(s) 202, 343, 509
AvaI CYCGRG 1 cut(s) 1072
AvaII GGWCC 1 cut(s) 361
BaeI ACNNNNGTAYC 2 cut(s) 544, 577
BauI CACGAG 2 cut(s) 853, 1167
BbsI GAAGAC 2 cut(s) 73, 506
BbvI GCAGC 4 cut(s) 154, 167, 217, 1153
BccI CCATC 4 cut(s) 158, 857, 949, 953
BceAI ACGGC 1 cut(s) 410
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BciT130I CCWGG 1 cut(s) 894
BcnI CCSGG 3 cut(s) 821, 1073, 1074
BcoDI GTCTC 2 cut(s) 13, 537
BfaI CTAG 2 cut(s) 164, 551
BfoI RGCGCY 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 410
BglII AGATCT 1 cut(s) 1129
BisI GCNGC 7 cut(s) 168, 181, 184, 231, 868, 871, 1142
BlpI GCTNAGC 1 cut(s) 234
BlsI GCNGC 7 cut(s) 169, 182, 185, 232, 869, 872, 1143
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 4 cut(s) 821, 894, 1073, 1074
Bme18I GGWCC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 1072
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 2 cut(s) 254, 363
BmrFI CCNGG 4 cut(s) 821, 894, 1073, 1074
BmsI GCATC 4 cut(s) 654, 673, 706, 906
BpiI GAAGAC 2 cut(s) 73, 506
Bpu1102I GCTNAGC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 1027
BpuMI CCSGG 3 cut(s) 821, 1073, 1074
BsaAI YACGTR 1 cut(s) 994
BsaJI CCNNGG 2 cut(s) 892, 1072
BsaWI WCCGGW 1 cut(s) 472
Bsc4I CCNNNNNNNGG 5 cut(s) 201, 245, 248, 319, 855
Bse1I ACTGG 3 cut(s) 460, 981, 1096
Bse3DI GCAATG 1 cut(s) 1146
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 1 cut(s) 894
BseDI CCNNGG 2 cut(s) 892, 1072
BseGI GGATG 2 cut(s) 721, 964
BseLI CCNNNNNNNGG 5 cut(s) 201, 245, 248, 319, 855
BseMI GCAATG 1 cut(s) 1146
BseNI ACTGG 3 cut(s) 460, 981, 1096
BseRI GAGGAG 1 cut(s) 58
BseX3I CGGCCG 1 cut(s) 868
BseXI GCAGC 4 cut(s) 154, 167, 217, 1153
Bsh1236I CGCG 2 cut(s) 369, 713
Bsh1285I CGRYCG 1 cut(s) 871
BshFI GGCC 1 cut(s) 870
BsiEI CGRYCG 1 cut(s) 871
BsiHKCI CYCGRG 1 cut(s) 1072
BsiSI CCGG 3 cut(s) 473, 821, 1073
BslFI GGGAC 1 cut(s) 626
BslI CCNNNNNNNGG 5 cut(s) 201, 245, 248, 319, 855
BsmAI GTCTC 2 cut(s) 13, 537
BsmFI GGGAC 1 cut(s) 626
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 1 cut(s) 870
BsoBI CYCGRG 1 cut(s) 1072
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
Bsp1720I GCTNAGC 1 cut(s) 234
BspACI CCGC 9 cut(s) 183, 202, 249, 354, 698, 713, 719, 867, 871
BspANI GGCC 1 cut(s) 870
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 2 cut(s) 369, 713
BspLI GGNNCC 2 cut(s) 254, 363
BspPI GGATC 1 cut(s) 742
BspTI CTTAAG 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 1146
BsrI ACTGG 3 cut(s) 460, 981, 1096
BssECI CCNNGG 2 cut(s) 892, 1072
BssMI GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
BssSI CACGAG 2 cut(s) 853, 1167
Bst2BI CACGAG 2 cut(s) 853, 1167
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 6 cut(s) 92, 113, 292, 332, 635, 1088
Bst6I CTCTTC 1 cut(s) 64
BstAFI CTTAAG 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 994
BstDEI CTNAG 2 cut(s) 234, 492
BstF5I GGATG 2 cut(s) 721, 964
BstFNI CGCG 2 cut(s) 369, 713
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 3 cut(s) 140, 230, 371
BstKTI GATC 6 cut(s) 159, 297, 385, 750, 1125, 1132
BstMAI GTCTC 2 cut(s) 13, 537
BstMBI GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
BstMCI CGRYCG 1 cut(s) 871
BstMWI GCNNNNNNNGC 2 cut(s) 484, 1157
BstNI CCWGG 1 cut(s) 894
BstNSI RCATGY 1 cut(s) 1064
BstSCI CCNGG 4 cut(s) 819, 892, 1071, 1072
BstUI CGCG 2 cut(s) 369, 713
BstV1I GCAGC 4 cut(s) 154, 167, 217, 1153
BstV2I GAAGAC 2 cut(s) 73, 506
BstX2I RGATCY 1 cut(s) 1129
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 1 cut(s) 1129
BstZI CGGCCG 1 cut(s) 868
BsuRI GGCC 1 cut(s) 870
BtsCI GGATG 2 cut(s) 721, 964
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 3 cut(s) 288, 453, 1161
CciNI GCGGCCGC 1 cut(s) 868
CfoI GCGC 3 cut(s) 140, 230, 371
Cfr13I GGNCC 1 cut(s) 361
Cfr9I CCCGGG 1 cut(s) 1072
CseI GACGC 1 cut(s) 205
Csp6I GTAC 6 cut(s) 192, 328, 709, 811, 889, 1089
CviAII CATG 5 cut(s) 388, 449, 616, 935, 1061
CviQI GTAC 6 cut(s) 192, 328, 709, 811, 889, 1089
DdeI CTNAG 2 cut(s) 234, 492
DpnI GATC 6 cut(s) 158, 296, 384, 749, 1124, 1131
DpnII GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
EaeI YGGCCR 1 cut(s) 868
EagI CGGCCG 1 cut(s) 868
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
EclXI CGGCCG 1 cut(s) 868
Eco47I GGWCC 1 cut(s) 361
Eco52I CGGCCG 1 cut(s) 868
Eco57I CTGAAG 2 cut(s) 541, 799
Eco88I CYCGRG 1 cut(s) 1072
EcoRII CCWGG 1 cut(s) 892
EcoT22I ATGCAT 1 cut(s) 688
FaeI CATG 5 cut(s) 391, 452, 619, 938, 1064
FalI AAGNNNNNCTT 2 cut(s) 662, 694
FaqI GGGAC 1 cut(s) 626
FatI CATG 5 cut(s) 387, 448, 615, 934, 1060
FauI CCCGC 2 cut(s) 242, 705
Fnu4HI GCNGC 7 cut(s) 168, 181, 184, 231, 868, 871, 1142
FokI GGATG 2 cut(s) 728, 971
Fsp4HI GCNGC 7 cut(s) 168, 181, 184, 231, 868, 871, 1142
FspBI CTAG 2 cut(s) 164, 551
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 3 cut(s) 139, 229, 370
GluI GCNGC 7 cut(s) 168, 181, 184, 231, 868, 871, 1142
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 1 cut(s) 870
HapII CCGG 3 cut(s) 473, 821, 1073
HgaI GACGC 1 cut(s) 205
HhaI GCGC 3 cut(s) 140, 230, 371
Hin1II CATG 5 cut(s) 391, 452, 619, 938, 1064
Hin6I GCGC 3 cut(s) 138, 228, 369
HinP1I GCGC 3 cut(s) 138, 228, 369
HincII GTYRAC 1 cut(s) 98
HindII GTYRAC 1 cut(s) 98
HinfI GANTC 7 cut(s) 117, 173, 260, 496, 742, 859, 920
HpaII CCGG 3 cut(s) 473, 821, 1073
HphI GGTGA 3 cut(s) 202, 343, 509
Hpy166II GTNNAC 2 cut(s) 98, 826
Hpy188I TCNGA 4 cut(s) 161, 609, 747, 1127
Hpy188III TCNNGA 6 cut(s) 154, 302, 430, 464, 473, 1018
Hpy8I GTNNAC 2 cut(s) 98, 826
Hpy99I CGWCG 2 cut(s) 96, 431
HpyAV CCTTC 5 cut(s) 503, 758, 790, 794, 833
HpyCH4III ACNGT 6 cut(s) 92, 113, 292, 332, 635, 1088
HpyCH4IV ACGT 1 cut(s) 993
HpyCH4V TGCA 2 cut(s) 686, 1172
HpyF10VI GCNNNNNNNGC 2 cut(s) 484, 1157
HpyF3I CTNAG 2 cut(s) 234, 492
HpySE526I ACGT 1 cut(s) 993
Hsp92II CATG 5 cut(s) 391, 452, 619, 938, 1064
HspAI GCGC 3 cut(s) 138, 228, 369
Kpn2I TCCGGA 1 cut(s) 472
Kzo9I GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 4 cut(s) 154, 167, 217, 1153
LweI GCATC 4 cut(s) 654, 673, 706, 906
MaeI CTAG 2 cut(s) 164, 551
MaeII ACGT 1 cut(s) 993
MaeIII GTNAC 5 cut(s) 349, 451, 629, 1001, 1039
MalI GATC 6 cut(s) 158, 296, 384, 749, 1124, 1131
MboI GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
MboII GAAGA 5 cut(s) 51, 54, 73, 506, 1132
MflI RGATCY 1 cut(s) 1129
MluCI AATT 5 cut(s) 37, 590, 603, 965, 1025
MlyI GAGTC 3 cut(s) 167, 736, 914
MmeI TCCRAC 2 cut(s) 288, 1093
Mph1103I ATGCAT 1 cut(s) 688
MroI TCCGGA 1 cut(s) 472
MroXI GAANNNNTTC 2 cut(s) 513, 732
MseI TTAA 3 cut(s) 53, 411, 807
MslI CAYNNNNRTG 1 cut(s) 728
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 3 cut(s) 473, 821, 1073
MspR9I CCNGG 4 cut(s) 821, 894, 1073, 1074
Mva1269I GAATGC 1 cut(s) 686
MvaI CCWGG 1 cut(s) 894
MvnI CGCG 2 cut(s) 369, 713
MwoI GCNNNNNNNGC 2 cut(s) 484, 1157
NciI CCSGG 3 cut(s) 821, 1073, 1074
NdeII GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
NlaIII CATG 5 cut(s) 391, 452, 619, 938, 1064
NlaIV GGNNCC 2 cut(s) 254, 363
NmuCI GTSAC 5 cut(s) 349, 451, 629, 1001, 1039
NotI GCGGCCGC 1 cut(s) 868
NsbI TGCGCA 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 1 cut(s) 1064
PctI GAATGC 1 cut(s) 686
PdmI GAANNNNTTC 2 cut(s) 513, 732
PfeI GAWTC 4 cut(s) 117, 260, 496, 859
PflMI CCANNNNNTGG 2 cut(s) 319, 855
PkrI GCNGC 7 cut(s) 169, 182, 185, 232, 869, 872, 1143
PleI GAGTC 3 cut(s) 167, 736, 914
PpsI GAGTC 3 cut(s) 167, 736, 914
Ppu21I YACGTR 1 cut(s) 994
PsiI TTATAA 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 892
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 2 cut(s) 254, 363
PspPI GGNCC 1 cut(s) 361
PsuI RGATCY 1 cut(s) 1129
RsaI GTAC 6 cut(s) 193, 329, 710, 812, 890, 1090
RsaNI GTAC 6 cut(s) 192, 328, 709, 811, 889, 1089
RseI CAYNNNNRTG 1 cut(s) 728
SaqAI TTAA 3 cut(s) 53, 411, 807
SatI GCNGC 7 cut(s) 168, 181, 184, 231, 868, 871, 1142
Sau3AI GATC 6 cut(s) 156, 294, 382, 747, 1122, 1129
Sau96I GGNCC 1 cut(s) 361
ScaI AGTACT 1 cut(s) 812
SchI GAGTC 3 cut(s) 167, 736, 914
ScrFI CCNGG 4 cut(s) 821, 894, 1073, 1074
SfaNI GCATC 4 cut(s) 654, 673, 706, 906
SinI GGWCC 1 cut(s) 361
SmaI CCCGGG 1 cut(s) 1074
SmiMI CAYNNNNRTG 1 cut(s) 728
SmlI CTYRAG 2 cut(s) 410, 1006
SmoI CTYRAG 2 cut(s) 410, 1006
Sse9I AATT 5 cut(s) 37, 590, 603, 965, 1025
SsiI CCGC 9 cut(s) 183, 202, 249, 354, 698, 713, 719, 867, 871
SspMI CTAG 2 cut(s) 164, 551
StyD4I CCNGG 4 cut(s) 819, 892, 1071, 1072
TaaI ACNGT 6 cut(s) 92, 113, 292, 332, 635, 1088
TaiI ACGT 1 cut(s) 996
TaqI TCGA 8 cut(s) 429, 507, 660, 681, 693, 834, 930, 1019
TasI AATT 5 cut(s) 37, 590, 603, 965, 1025
TatI WGTACW 1 cut(s) 810
TauI GCSGC 3 cut(s) 186, 870, 873
TfiI GAWTC 4 cut(s) 117, 260, 496, 859
Tru1I TTAA 3 cut(s) 53, 411, 807
Tru9I TTAA 3 cut(s) 53, 411, 807
TscAI CASTG 3 cut(s) 295, 460, 1168
TseFI GTSAC 5 cut(s) 349, 451, 629, 1001, 1039
TseI GCWGC 4 cut(s) 167, 180, 230, 1141
Tsp45I GTSAC 5 cut(s) 349, 451, 629, 1001, 1039
TspDTI ATGAA 3 cut(s) 679, 809, 852
TspGWI ACGGA 1 cut(s) 1047
TspMI CCCGGG 1 cut(s) 1072
TspRI CASTG 3 cut(s) 295, 460, 1168
Van91I CCANNNNNTGG 2 cut(s) 319, 855
Vha464I CTTAAG 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 361
XapI RAATTY 3 cut(s) 590, 603, 1025
XceI RCATGY 1 cut(s) 1064
XcmI CCANNNNNNNNNTGG 1 cut(s) 1109
XmaI CCCGGG 1 cut(s) 1072
XmnI GAANNNNTTC 2 cut(s) 513, 732
XspI CTAG 2 cut(s) 164, 551
ZrmI AGTACT 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.