MD00G1068500.v1.1

Phosphoribulokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0014052
Physical Location & Seq
Reverse (-)
13445550 .. 13448978
3429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1068500.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGCGATTTGTGCAGTGCACACAAGTGGCCAGCTGAACCCGACGTGTTCATTTTCCTCGTCCTTGGGATTCAACGGCCAAAAGCAGCAGCAGGTGGCGGTGTTTTACAAGCAGAGAAGCAGCAGCAGCAGGAGAGTTGGAGTGAGTGTGATAAGATGCGAGCAAGAGACGATTGTTATCGGTTTAGCAGCAGACTCAGGGTGTGGGAAGAGCACATTCATGAGGAGGCTGACGAGCGTGTTTGGAGGAGCAGCGGAGCCGCCTAAAGGTGGAAACCCTGATTCCAACACGCTCATCAGCGACACCACGACGGTGATATGCTTGGATGATTACCATTCCCTGGACAGGACAGGGAGGAAGGAGAAAGGAGTGACGGCGCTGGACCCAAGAGCAAACGACTTCGATCTTATGTATGAACAGGTGAAGGCCATCAAGGAAGGCAAGGCTGTTGAGAAACCTATTTACAACCATGTTTCTGGTCTCTTGGATCCTCCTGAGCTCATCAAGCCCCCCAAGATTCTTGTCATTGAAGGTTTACACCCAATGTATGATCAGCGAGTTAGGGACTTATTGGACTTCAGTATCTACTTGGACATCAGCAATGAGGTTAAGTTTGCATGGAAAATTCAGAGAGATATGGCGGAGCGTGGGCACAGTCTTGAGAGCATCAAAGCCAGTATTGAAGCCCGAAAGCCTGACTTCGATGCTTATATTGATCCCCAAAAGCAGTATGCAGATGCAGTTATAGAAGTGTTGCCAACCCAATTGATTCCAGGAGACAATGAGGGGAAGGTGTTGAGAGTGAGGTTGATAATGAAGGAAGGTGTGAAATATTTCAACCCAGTCTACTTGTTTGATGAGGGCTCCACCATCTCATGGATTCCCTGTGGAAGGAAACTCACCTGCTCTTACCCTGGAATCAAATTCAACTACGGCCCCGACACTTACTTTGGCAACGAGGTGTCGGTGTTGGAGATGGACGGGCAATTTGACAGATTAGATGAGCTGATCTATGTAGAGAGCCATCTAAGCAACATCTCCACCAAATTCTATGGAGAAGTCACCCAGCAAATGTTGAAGCATTCTGATTTCCCAGGGAGCAACAACGGAACCGGTCTCTTCCAAACCATCGTCGGATTGAAGATACGCGACCTCTACGAGCAGCTCATTGCAGCCAGGGCCAAAACTCCAGCAGAAGCAAAAGCTTAG

Protein Analysis

403

Amino Acids

44.74

Weight (kDa)

6.04

Isoelectric Point (pI)

27.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRK PF00485 59 - 257 5.1e-80 Phosphoribulokinase / Uridine kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32060
fragaria_vesca FvH4_5g23520
malus_domestica MD00G1068500.v1.1 MD06G1023500.v1.1
prunus_persica Prupe.5G024600_v2.0.a1
pyrus_communis pycom06g01910 pycom16g25320
rosa_chinensis RchiOBHm_Chr7g0212791
rosa_laevigata RLG00000002868
rosa_multiflora Rmu_sc0000394.1_g000036
rosa_roxburghii Rroxscaffold_3G00246550
rosa_rugosa Rorug07G0136800
rosa_samantha Rh7AG268300 Rh7BG263700 Rh7CG287300
rosa_wichuraiana Rw7G023180 Rw7G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 82, 911
Acc36I ACCTGC 2 cut(s) 82, 911
AccB7I CCANNNNNTGG 2 cut(s) 340, 876
AccI GTMKAC 1 cut(s) 846
AccII CGCG 1 cut(s) 1149
AciI CCGC 4 cut(s) 98, 254, 260, 641
AclWI GGATC 3 cut(s) 482, 495, 710
AcoI YGGCCR 2 cut(s) 28, 76
AcsI RAATTY 3 cut(s) 624, 923, 1046
AcuI CTGAAG 1 cut(s) 562
AfiI CCNNNNNNNGG 6 cut(s) 266, 269, 340, 345, 876, 891
AflIII ACRYGT 1 cut(s) 44
AgeI ACCGGT 1 cut(s) 1112
AgsI TTSAA 7 cut(s) 73, 530, 683, 838, 928, 1078, 1141
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 5 cut(s) 339, 772, 913, 1093, 1175
AleI CACNNNNGTG 2 cut(s) 24, 311
AluBI AGCT 5 cut(s) 34, 499, 1006, 1165, 1205
AluI AGCT 5 cut(s) 34, 499, 1006, 1165, 1205
Alw21I GWGCWC 3 cut(s) 21, 215, 501
Alw26I GTCTC 4 cut(s) 161, 485, 771, 1121
Alw44I GTGCAC 1 cut(s) 17
AlwI GGATC 3 cut(s) 482, 495, 710
AoxI GGCC 5 cut(s) 28, 76, 426, 934, 1179
ApaLI GTGCAC 1 cut(s) 17
ApeKI GCWGC 9 cut(s) 85, 88, 120, 123, 126, 188, 251, 1162, 1172
ApoI RAATTY 3 cut(s) 624, 923, 1046
ArsI GACNNNNNNTTYG 4 cut(s) 932, 964, 971, 1003
AsiGI ACCGGT 1 cut(s) 1112
Asp700I GAANNNNTTC 1 cut(s) 833
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 3 cut(s) 382, 935, 1179
AsuHPI GGTGA 4 cut(s) 325, 433, 892, 1054
AvaII GGWCC 1 cut(s) 382
BaeGI GKGCMC 2 cut(s) 21, 654
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 487
BanII GRGCYC 2 cut(s) 501, 866
Bbv12I GWGCWC 3 cut(s) 21, 215, 501
BbvI GCAGC 9 cut(s) 97, 100, 132, 135, 138, 200, 263, 1174, 1184
BccI CCATC 5 cut(s) 437, 878, 970, 1032, 1136
BceAI ACGGC 3 cut(s) 91, 390, 949
BciT130I CCWGG 5 cut(s) 341, 774, 915, 1095, 1177
BclI TGATCA 1 cut(s) 550
BcoDI GTCTC 4 cut(s) 161, 485, 771, 1121
BfoI RGCGCY 1 cut(s) 380
BfuAI ACCTGC 2 cut(s) 82, 911
Bme1390I CCNGG 5 cut(s) 341, 774, 915, 1095, 1177
Bme18I GGWCC 1 cut(s) 382
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 3 cut(s) 382, 935, 1179
BmiI GGNNCC 6 cut(s) 258, 384, 489, 865, 937, 1111
BmrFI CCNGG 5 cut(s) 341, 774, 915, 1095, 1177
BmrI ACTGGG 1 cut(s) 836
BmsI GCATC 4 cut(s) 146, 675, 694, 727
BmuI ACTGGG 1 cut(s) 836
BpmI CTGGAG 1 cut(s) 1173
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 680
BsaBI GATNNNNATC 1 cut(s) 176
BsaI GGTCTC 2 cut(s) 485, 1121
BsaJI CCNNGG 6 cut(s) 63, 339, 913, 1093, 1094, 1176
BsaWI WCCGGW 1 cut(s) 1112
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 6 cut(s) 266, 269, 340, 345, 876, 891
Bse118I RCCGGY 1 cut(s) 1112
Bse1I ACTGG 2 cut(s) 675, 842
Bse3DI GCAATG 2 cut(s) 607, 1167
Bse8I GATNNNNATC 1 cut(s) 176
BseBI CCWGG 5 cut(s) 341, 774, 915, 1095, 1177
BseDI CCNNGG 6 cut(s) 63, 339, 913, 1093, 1094, 1176
BseGI GGATG 1 cut(s) 331
BseJI GATNNNNATC 1 cut(s) 176
BseLI CCNNNNNNNGG 6 cut(s) 266, 269, 340, 345, 876, 891
BseMI GCAATG 2 cut(s) 607, 1167
BseMII CTCAG 2 cut(s) 210, 486
BseNI ACTGG 2 cut(s) 675, 842
BseRI GAGGAG 2 cut(s) 238, 261
BseSI GKGCMC 2 cut(s) 21, 654
BseXI GCAGC 9 cut(s) 97, 100, 132, 135, 138, 200, 263, 1174, 1184
BseYI CCCAGC 1 cut(s) 1065
BsgI GTGCAG 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 1149
BshFI GGCC 5 cut(s) 30, 78, 428, 936, 1181
BshTI ACCGGT 1 cut(s) 1112
BsiHKAI GWGCWC 3 cut(s) 21, 215, 501
BsiSI CCGG 1 cut(s) 1113
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 6 cut(s) 266, 269, 340, 345, 876, 891
BsmAI GTCTC 4 cut(s) 161, 485, 771, 1121
BsmBI CGTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 5 cut(s) 30, 78, 428, 936, 1181
Bso31I GGTCTC 2 cut(s) 485, 1121
Bsp1286I GDGCHC 5 cut(s) 21, 215, 501, 654, 866
Bsp143I GATC 5 cut(s) 403, 487, 550, 715, 1008
BspACI CCGC 4 cut(s) 98, 254, 260, 641
BspANI GGCC 5 cut(s) 30, 78, 428, 936, 1181
BspCNI CTCAG 2 cut(s) 209, 487
BspFNI CGCG 1 cut(s) 1149
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 6 cut(s) 258, 384, 489, 865, 937, 1111
BspMI ACCTGC 2 cut(s) 82, 911
BspPI GGATC 3 cut(s) 482, 495, 710
BspQI GCTCTTC 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 485, 1121
BsrDI GCAATG 2 cut(s) 607, 1167
BsrFI RCCGGY 1 cut(s) 1112
BsrI ACTGG 2 cut(s) 675, 842
BssAI RCCGGY 1 cut(s) 1112
BssECI CCNNGG 6 cut(s) 63, 339, 913, 1093, 1094, 1176
BssMI GATC 5 cut(s) 403, 487, 550, 715, 1008
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 5 cut(s) 341, 774, 915, 1095, 1177
Bst4CI ACNGT 2 cut(s) 313, 656
Bst6I CTCTTC 2 cut(s) 203, 1124
BstC8I GCNNGC 2 cut(s) 32, 161
BstDEI CTNAG 4 cut(s) 196, 495, 1028, 1206
BstENI CCTNNNNNAGG 1 cut(s) 889
BstF5I GGATG 1 cut(s) 331
BstFNI CGCG 1 cut(s) 1149
BstH2I RGCGCY 1 cut(s) 380
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 5 cut(s) 406, 490, 553, 718, 1011
BstMAI GTCTC 4 cut(s) 161, 485, 771, 1121
BstMBI GATC 5 cut(s) 403, 487, 550, 715, 1008
BstMWI GCNNNNNNNGC 5 cut(s) 11, 126, 505, 1029, 1178
BstNI CCWGG 5 cut(s) 341, 774, 915, 1095, 1177
BstSCI CCNGG 5 cut(s) 339, 772, 913, 1093, 1175
BstSLI GKGCMC 2 cut(s) 21, 654
BstUI CGCG 1 cut(s) 1149
BstV1I GCAGC 9 cut(s) 97, 100, 132, 135, 138, 200, 263, 1174, 1184
BstX2I RGATCY 1 cut(s) 487
BstXI CCANNNNNNTGG 1 cut(s) 476
BstYI RGATCY 1 cut(s) 487
BsuRI GGCC 5 cut(s) 30, 78, 428, 936, 1181
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 1 cut(s) 331
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 1 cut(s) 21
BveI ACCTGC 2 cut(s) 82, 911
Cac8I GCNNGC 2 cut(s) 32, 161
CciI TCATGA 1 cut(s) 219
CfoI GCGC 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 1112
Cfr13I GGNCC 3 cut(s) 382, 935, 1179
CspAI ACCGGT 1 cut(s) 1112
CviAII CATG 4 cut(s) 220, 470, 618, 876
DdeI CTNAG 4 cut(s) 196, 495, 1028, 1206
DpnI GATC 5 cut(s) 405, 489, 552, 717, 1010
DpnII GATC 5 cut(s) 403, 487, 550, 715, 1008
EaeI YGGCCR 2 cut(s) 28, 76
Eam1104I CTCTTC 2 cut(s) 203, 1124
EarI CTCTTC 2 cut(s) 203, 1124
EciI GGCGGA 1 cut(s) 656
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 2 cut(s) 501, 866
Eco31I GGTCTC 2 cut(s) 485, 1121
Eco47I GGWCC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 562
EcoICRI GAGCTC 1 cut(s) 499
EcoNI CCTNNNNNAGG 1 cut(s) 889
EcoRII CCWGG 5 cut(s) 339, 772, 913, 1093, 1175
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 2 cut(s) 501, 866
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 161
FaeI CATG 4 cut(s) 223, 473, 621, 879
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FaqI GGGAC 1 cut(s) 578
FatI CATG 4 cut(s) 219, 469, 617, 875
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 1 cut(s) 846
FokI GGATG 1 cut(s) 338
FriOI GRGCYC 2 cut(s) 501, 866
GlaI GCGC 1 cut(s) 378
GsaI CCCAGC 1 cut(s) 1069
GsuI CTGGAG 1 cut(s) 1173
HaeII RGCGCY 1 cut(s) 380
HaeIII GGCC 5 cut(s) 30, 78, 428, 936, 1181
HapII CCGG 1 cut(s) 1113
HhaI GCGC 1 cut(s) 379
Hin1II CATG 4 cut(s) 223, 473, 621, 879
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HindIII AAGCTT 1 cut(s) 1203
HinfI GANTC 7 cut(s) 69, 194, 281, 517, 769, 880, 918
HpaII CCGG 1 cut(s) 1113
HphI GGTGA 4 cut(s) 325, 433, 892, 1054
Hpy166II GTNNAC 3 cut(s) 19, 536, 847
Hpy188I TCNGA 3 cut(s) 630, 1087, 1136
Hpy188III TCNNGA 3 cut(s) 220, 494, 659
Hpy8I GTNNAC 3 cut(s) 19, 536, 847
Hpy99I CGWCG 3 cut(s) 46, 313, 1136
HpyAV CCTTC 8 cut(s) 352, 418, 431, 524, 784, 811, 815, 885
HpyCH4III ACNGT 2 cut(s) 313, 656
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 6 cut(s) 14, 19, 617, 734, 740, 1172
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 126, 505, 1029, 1178
HpyF3I CTNAG 4 cut(s) 196, 495, 1028, 1206
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 4 cut(s) 223, 473, 621, 879
HspAI GCGC 1 cut(s) 377
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 5 cut(s) 403, 487, 550, 715, 1008
LguI GCTCTTC 1 cut(s) 203
LmnI GCTCC 5 cut(s) 248, 256, 643, 869, 1098
Lsp1109I GCAGC 9 cut(s) 97, 100, 132, 135, 138, 200, 263, 1174, 1184
LweI GCATC 4 cut(s) 146, 675, 694, 727
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 2 cut(s) 370, 1060
MalI GATC 5 cut(s) 405, 489, 552, 717, 1010
MboI GATC 5 cut(s) 403, 487, 550, 715, 1008
MboII GAAGA 3 cut(s) 220, 1111, 1153
MfeI CAATTG 1 cut(s) 764
MflI RGATCY 1 cut(s) 487
MhlI GDGCHC 5 cut(s) 21, 215, 501, 654, 866
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 5 cut(s) 624, 764, 923, 986, 1046
MluNI TGGCCA 1 cut(s) 30
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 4 cut(s) 118, 309, 951, 1114
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 833
MscI TGGCCA 1 cut(s) 30
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 3 cut(s) 24, 218, 311
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 2 cut(s) 34, 254
MspI CCGG 1 cut(s) 1113
MspR9I CCNGG 5 cut(s) 341, 774, 915, 1095, 1177
MunI CAATTG 1 cut(s) 764
Mva1269I GAATGC 1 cut(s) 1081
MvaI CCWGG 5 cut(s) 341, 774, 915, 1095, 1177
MvnI CGCG 1 cut(s) 1149
MwoI GCNNNNNNNGC 5 cut(s) 11, 126, 505, 1029, 1178
NdeII GATC 5 cut(s) 403, 487, 550, 715, 1008
NlaIII CATG 4 cut(s) 223, 473, 621, 879
NlaIV GGNNCC 6 cut(s) 258, 384, 489, 865, 937, 1111
NmuCI GTSAC 2 cut(s) 370, 1060
OliI CACNNNNGTG 2 cut(s) 24, 311
PagI TCATGA 1 cut(s) 219
PaqCI CACCTGC 2 cut(s) 82, 911
PasI CCCWGGG 1 cut(s) 1094
PciSI GCTCTTC 1 cut(s) 203
PctI GAATGC 1 cut(s) 1081
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 6 cut(s) 69, 281, 517, 769, 880, 918
PflMI CCANNNNNTGG 2 cut(s) 340, 876
PfoI TCCNGGA 1 cut(s) 772
PinAI ACCGGT 1 cut(s) 1112
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 5 cut(s) 339, 772, 913, 1093, 1175
PspFI CCCAGC 1 cut(s) 1065
PspGI CCWGG 5 cut(s) 339, 772, 913, 1093, 1175
PspN4I GGNNCC 6 cut(s) 258, 384, 489, 865, 937, 1111
PspPI GGNCC 3 cut(s) 382, 935, 1179
PsuI RGATCY 1 cut(s) 487
PvuII CAGCTG 1 cut(s) 34
RseI CAYNNNNRTG 3 cut(s) 24, 218, 311
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 609
Sau3AI GATC 5 cut(s) 403, 487, 550, 715, 1008
Sau96I GGNCC 3 cut(s) 382, 935, 1179
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 5 cut(s) 341, 774, 915, 1095, 1177
SduI GDGCHC 5 cut(s) 21, 215, 501, 654, 866
SfaNI GCATC 4 cut(s) 146, 675, 694, 727
SinI GGWCC 1 cut(s) 382
SmiMI CAYNNNNRTG 3 cut(s) 24, 218, 311
SmlI CTYRAG 1 cut(s) 659
SmoI CTYRAG 1 cut(s) 659
Sse9I AATT 5 cut(s) 624, 764, 923, 986, 1046
SsiI CCGC 4 cut(s) 98, 254, 260, 641
SspI AATATT 1 cut(s) 833
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 5 cut(s) 339, 772, 913, 1093, 1175
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 2 cut(s) 313, 656
TaiI ACGT 1 cut(s) 47
TaqI TCGA 2 cut(s) 402, 702
TasI AATT 5 cut(s) 624, 764, 923, 986, 1046
TauI GCSGC 1 cut(s) 262
TfiI GAWTC 6 cut(s) 69, 281, 517, 769, 880, 918
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 21
TseFI GTSAC 2 cut(s) 370, 1060
TseI GCWGC 9 cut(s) 85, 88, 120, 123, 126, 188, 251, 1162, 1172
Tsp45I GTSAC 2 cut(s) 370, 1060
TspDTI ATGAA 4 cut(s) 39, 208, 429, 830
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 1 cut(s) 21
Van91I CCANNNNNTGG 2 cut(s) 340, 876
VneI GTGCAC 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 382
XagI CCTNNNNNAGG 1 cut(s) 889
XapI RAATTY 3 cut(s) 624, 923, 1046
XmiI GTMKAC 1 cut(s) 846
XmnI GAANNNNTTC 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.