pycom06g01910

Phosphoribulokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014052
Physical Location & Seq
Forward (+)
2397569 .. 2400500
2932 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g01910.5

Sequence Viewer

Length: 1215 bp
ATGGCGATTTGTGCAGTGCACACAAGTGGCCAGCTGAACCCGACGTGTTCGTTTTCCTCCTCGTTGGGGTTCAGCGGCCAAAAGCAGCAGCAGGTGGCGGTGTTCTACAAGCAGAGAAGCAGCAGCAGCAGGAGATCAGTTGGAGTGAGTGTGATAAGATGCGAGCAAGAGACGATTGTTATCGGTTTAGCAGCGGACTCAGGGTGTGGGAAGAGCACATTCATGAGGAGGCTGACGAGCGTGTTTGGAGGAGCAGCGGAGCCGCCTAAAGGAGGAAACCCTGACTCCAACACGCTCATCAGCGACACCACGACGGTGATATGCTTGGATGATTACCATTCCCTGGACAGGACAGGGAGGAAGGAGAAAGGAGTGACCGCGCTGGACCCAAGAGCTAACGACTTCGATCTTATGTATGAGCAGGTGAAGGCCATCAAGGAAGGGAAGGCTGTTGAGAAGCCTATTTACAACCATGTTTCTGGTCTCTTCGATCCTCCCGAGCTCATCAAGCCCCCCAAGATTCTTGTCATTGAAGGTTTACATCCAATGTACGATCAGCGAGTTAGGGATCTGTTGGACTTCAGCATCTACTTGGACATCAGCAATGAGGTTAAATTTGCATGGAAAATTCAGAGGGACATGGCGGAGCGTGGACACAGTCTCGAGAGCATCAAAGCCAGTATTGAAGCCCGAAAGCCTGATTTCGATGCTTATATTGATCCACAAAAGCAGTACGCAGATGCAGTGATAGAAGTGTTGCCAACCCAATTGATTCCAGGTGACAATGAGGGGAAGGTGTTGAGAGTGAGGTTGATTATGAAGGAAGGTGTGAAATATTTCAACCCAGTCTACTTGTTTGATGAGGGTTCCACCATCTCATGGATTCCCTGTGGAAGGAAGCTCACCTGCTCTTACCCTGGAATCAAATTCAACTATGGCCCTGACACTTACTTCAGCAACGAGGTGTCATTGTTGGAGATGGATGGGCAATTTGACAGATTAGATGAGCTGATCTATGTGGAGAGTCATCTAAGCAACATCTCCACCAAGTTCTATGGAGAAGTCACCCAACAAATGTTGAAGCACTCTGATTTCCCCGGCAGCAACAACGGAACTGGCCTCTTCCAAACCATCGTCGGATTGAAGATCCGGGACCTCTACGAGCAGCTTATTGCAGCCAGGGCCAAAACTCCAGTAGAAGCAACCAAAGCTTAG

Protein Analysis

405

Amino Acids

45.01

Weight (kDa)

6.04

Isoelectric Point (pI)

29.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32060
fragaria_vesca FvH4_5g23520
malus_domestica MD00G1068500.v1.1 MD06G1023500.v1.1
prunus_persica Prupe.5G024600_v2.0.a1
pyrus_communis pycom06g01910 pycom16g25320
rosa_chinensis RchiOBHm_Chr7g0212791
rosa_laevigata RLG00000002868
rosa_multiflora Rmu_sc0000394.1_g000036
rosa_roxburghii Rroxscaffold_3G00246550
rosa_rugosa Rorug07G0136800
rosa_samantha Rh7AG268300 Rh7BG263700 Rh7CG287300
rosa_wichuraiana Rw7G023180 Rw7G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 82, 412, 914
Acc36I ACCTGC 3 cut(s) 82, 412, 914
AccB7I CCANNNNNTGG 2 cut(s) 343, 879
AccI GTMKAC 1 cut(s) 849
AccII CGCG 1 cut(s) 380
AciI CCGC 7 cut(s) 75, 98, 194, 257, 263, 378, 644
AclWI GGATC 4 cut(s) 485, 576, 713, 1141
AcoI YGGCCR 2 cut(s) 28, 76
AcsI RAATTY 3 cut(s) 614, 627, 926
AcuI CTGAAG 2 cut(s) 565, 937
AfaI GTAC 2 cut(s) 551, 734
AfiI CCNNNNNNNGG 7 cut(s) 66, 269, 272, 343, 348, 879, 894
AflIII ACRYGT 1 cut(s) 44
AgsI TTSAA 6 cut(s) 533, 686, 841, 931, 1081, 1144
AjiI CACGTC 1 cut(s) 45
AjnI CCWGG 4 cut(s) 342, 775, 916, 1178
AleI CACNNNNGTG 2 cut(s) 24, 314
AluBI AGCT 7 cut(s) 34, 395, 502, 901, 1009, 1168, 1211
AluI AGCT 7 cut(s) 34, 395, 502, 901, 1009, 1168, 1211
Alw21I GWGCWC 3 cut(s) 21, 218, 504
Alw26I GTCTC 3 cut(s) 164, 488, 665
Alw44I GTGCAC 1 cut(s) 17
AlwI GGATC 4 cut(s) 485, 576, 713, 1141
Ama87I CYCGRG 2 cut(s) 497, 662
AoxI GGCC 6 cut(s) 28, 76, 429, 937, 1117, 1182
ApaLI GTGCAC 1 cut(s) 17
ApoI RAATTY 3 cut(s) 614, 627, 926
Asp700I GAANNNNTTC 1 cut(s) 836
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 4 cut(s) 385, 938, 1153, 1182
AsuC2I CCSGG 2 cut(s) 1098, 1151
AsuHPI GGTGA 5 cut(s) 328, 436, 791, 895, 1057
AvaI CYCGRG 2 cut(s) 497, 662
AvaII GGWCC 2 cut(s) 385, 1153
BaeGI GKGCMC 1 cut(s) 21
BalI TGGCCA 1 cut(s) 30
BanII GRGCYC 1 cut(s) 504
BarI GAAGNNNNNNTAC 2 cut(s) 449, 481
Bbv12I GWGCWC 3 cut(s) 21, 218, 504
BccI CCATC 5 cut(s) 440, 881, 973, 977, 1139
BciT130I CCWGG 4 cut(s) 344, 777, 918, 1180
BcnI CCSGG 2 cut(s) 1098, 1151
BcoDI GTCTC 3 cut(s) 164, 488, 665
BfuAI ACCTGC 3 cut(s) 82, 412, 914
Bme1390I CCNGG 6 cut(s) 344, 777, 918, 1098, 1151, 1180
Bme18I GGWCC 2 cut(s) 385, 1153
BmeT110I CYCGRG 2 cut(s) 497, 662
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 4 cut(s) 385, 938, 1153, 1182
BmiI GGNNCC 4 cut(s) 261, 387, 868, 1154
BmrFI CCNGG 6 cut(s) 344, 777, 918, 1098, 1151, 1180
BmrI ACTGGG 1 cut(s) 839
BmsI GCATC 5 cut(s) 149, 594, 678, 697, 730
BmuI ACTGGG 1 cut(s) 839
BpmI CTGGAG 1 cut(s) 1176
BpuMI CCSGG 2 cut(s) 1098, 1151
BsaBI GATNNNNATC 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 4 cut(s) 342, 916, 1096, 1179
BsaXI ACNNNNNCTCC 4 cut(s) 135, 165, 269, 299
Bsc4I CCNNNNNNNGG 7 cut(s) 66, 269, 272, 343, 348, 879, 894
Bse1I ACTGG 4 cut(s) 678, 845, 1120, 1193
Bse3DI GCAATG 1 cut(s) 610
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 4 cut(s) 344, 777, 918, 1180
BseDI CCNNGG 4 cut(s) 342, 916, 1096, 1179
BseGI GGATG 3 cut(s) 334, 541, 988
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 7 cut(s) 66, 269, 272, 343, 348, 879, 894
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 1 cut(s) 213
BseNI ACTGG 4 cut(s) 678, 845, 1120, 1193
BseRI GAGGAG 3 cut(s) 49, 241, 264
BseSI GKGCMC 1 cut(s) 21
BsgI GTGCAG 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 6 cut(s) 30, 78, 431, 939, 1119, 1184
BsiHKAI GWGCWC 3 cut(s) 21, 218, 504
BsiHKCI CYCGRG 2 cut(s) 497, 662
BsiSI CCGG 2 cut(s) 1098, 1150
BslFI GGGAC 2 cut(s) 650, 1166
BslI CCNNNNNNNGG 7 cut(s) 66, 269, 272, 343, 348, 879, 894
BsmAI GTCTC 3 cut(s) 164, 488, 665
BsmBI CGTCTC 1 cut(s) 164
BsmFI GGGAC 2 cut(s) 650, 1166
BsnI GGCC 6 cut(s) 30, 78, 431, 939, 1119, 1184
Bso31I GGTCTC 1 cut(s) 488
BsoBI CYCGRG 2 cut(s) 497, 662
Bsp1286I GDGCHC 3 cut(s) 21, 218, 504
Bsp143I GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
BspACI CCGC 7 cut(s) 75, 98, 194, 257, 263, 378, 644
BspANI GGCC 6 cut(s) 30, 78, 431, 939, 1119, 1184
BspCNI CTCAG 1 cut(s) 212
BspFNI CGCG 1 cut(s) 380
BspHI TCATGA 1 cut(s) 222
BspLI GGNNCC 4 cut(s) 261, 387, 868, 1154
BspMI ACCTGC 3 cut(s) 82, 412, 914
BspPI GGATC 4 cut(s) 485, 576, 713, 1141
BspQI GCTCTTC 1 cut(s) 206
BspTNI GGTCTC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 610
BsrI ACTGG 4 cut(s) 678, 845, 1120, 1193
BssECI CCNNGG 4 cut(s) 342, 916, 1096, 1179
BssMI GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
Bst2UI CCWGG 4 cut(s) 344, 777, 918, 1180
Bst4CI ACNGT 2 cut(s) 316, 659
Bst6I CTCTTC 3 cut(s) 206, 491, 1127
BstC8I GCNNGC 2 cut(s) 32, 164
BstDEI CTNAG 3 cut(s) 199, 1031, 1212
BstENI CCTNNNNNAGG 2 cut(s) 270, 892
BstF5I GGATG 3 cut(s) 334, 541, 988
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 8 cut(s) 137, 409, 493, 556, 571, 721, 1014, 1149
BstMAI GTCTC 3 cut(s) 164, 488, 665
BstMBI GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
BstMWI GCNNNNNNNGC 5 cut(s) 11, 126, 508, 1181, 1208
BstNI CCWGG 4 cut(s) 344, 777, 918, 1180
BstSCI CCNGG 6 cut(s) 342, 775, 916, 1096, 1149, 1178
BstSLI GKGCMC 1 cut(s) 21
BstUI CGCG 1 cut(s) 380
BstX2I RGATCY 2 cut(s) 568, 1146
BstXI CCANNNNNNTGG 1 cut(s) 479
BstYI RGATCY 2 cut(s) 568, 1146
BsuRI GGCC 6 cut(s) 30, 78, 431, 939, 1119, 1184
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 3 cut(s) 334, 541, 988
BtsI GCAGTG 2 cut(s) 21, 750
BtsIMutI CAGTG 2 cut(s) 21, 750
BveI ACCTGC 3 cut(s) 82, 412, 914
Cac8I GCNNGC 2 cut(s) 32, 164
CciI TCATGA 1 cut(s) 222
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 4 cut(s) 385, 938, 1153, 1182
Csp6I GTAC 2 cut(s) 550, 733
CviAII CATG 5 cut(s) 223, 473, 621, 640, 879
CviQI GTAC 2 cut(s) 550, 733
DdeI CTNAG 3 cut(s) 199, 1031, 1212
DpnI GATC 8 cut(s) 136, 408, 492, 555, 570, 720, 1013, 1148
DpnII GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
EaeI YGGCCR 2 cut(s) 28, 76
Eam1104I CTCTTC 3 cut(s) 206, 491, 1127
EarI CTCTTC 3 cut(s) 206, 491, 1127
EciI GGCGGA 1 cut(s) 659
Ecl136II GAGCTC 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 504
Eco31I GGTCTC 1 cut(s) 488
Eco47I GGWCC 2 cut(s) 385, 1153
Eco53kI GAGCTC 1 cut(s) 502
Eco57I CTGAAG 2 cut(s) 565, 937
Eco88I CYCGRG 2 cut(s) 497, 662
EcoICRI GAGCTC 1 cut(s) 502
EcoNI CCTNNNNNAGG 2 cut(s) 270, 892
EcoO109I RGGNCCY 1 cut(s) 1153
EcoRII CCWGG 4 cut(s) 342, 775, 916, 1178
EcoT38I GRGCYC 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 5 cut(s) 226, 476, 624, 643, 882
FaqI GGGAC 2 cut(s) 650, 1166
FatI CATG 5 cut(s) 222, 472, 620, 639, 878
FblI GTMKAC 1 cut(s) 849
FokI GGATG 3 cut(s) 341, 528, 995
FriOI GRGCYC 1 cut(s) 504
GlaI GCGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 1176
HaeIII GGCC 6 cut(s) 30, 78, 431, 939, 1119, 1184
HapII CCGG 2 cut(s) 1098, 1150
HhaI GCGC 1 cut(s) 382
Hin1II CATG 5 cut(s) 226, 476, 624, 643, 882
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HindIII AAGCTT 1 cut(s) 1209
HinfI GANTC 7 cut(s) 197, 284, 520, 772, 883, 921, 1024
HpaII CCGG 2 cut(s) 1098, 1150
HphI GGTGA 5 cut(s) 328, 436, 791, 895, 1057
Hpy166II GTNNAC 4 cut(s) 19, 539, 653, 850
Hpy188I TCNGA 3 cut(s) 633, 1090, 1139
Hpy188III TCNNGA 4 cut(s) 223, 497, 662, 664
Hpy8I GTNNAC 4 cut(s) 19, 539, 653, 850
Hpy99I CGWCG 3 cut(s) 46, 316, 1139
HpyAV CCTTC 9 cut(s) 355, 421, 434, 439, 527, 787, 814, 818, 888
HpyCH4III ACNGT 2 cut(s) 316, 659
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 5 cut(s) 14, 19, 620, 743, 1175
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 126, 508, 1181, 1208
HpyF3I CTNAG 3 cut(s) 199, 1031, 1212
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 5 cut(s) 226, 476, 624, 643, 882
HspAI GCGC 1 cut(s) 380
Kzo9I GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
LguI GCTCTTC 1 cut(s) 206
LmnI GCTCC 3 cut(s) 251, 259, 646
LweI GCATC 5 cut(s) 149, 594, 678, 697, 730
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 3 cut(s) 373, 779, 1063
MalI GATC 8 cut(s) 136, 408, 492, 555, 570, 720, 1013, 1148
MboI GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
MboII GAAGA 4 cut(s) 223, 478, 1114, 1156
MfeI CAATTG 1 cut(s) 767
MflI RGATCY 2 cut(s) 568, 1146
MhlI GDGCHC 3 cut(s) 21, 218, 504
MlsI TGGCCA 1 cut(s) 30
MluCI AATT 5 cut(s) 614, 627, 767, 926, 989
MluNI TGGCCA 1 cut(s) 30
MlyI GAGTC 3 cut(s) 191, 278, 1033
MmeI TCCRAC 5 cut(s) 121, 312, 555, 954, 1117
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 836
MscI TGGCCA 1 cut(s) 30
MseI TTAA 1 cut(s) 612
MslI CAYNNNNRTG 3 cut(s) 24, 221, 314
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 4 cut(s) 34, 75, 194, 257
MspI CCGG 2 cut(s) 1098, 1150
MspR9I CCNGG 6 cut(s) 344, 777, 918, 1098, 1151, 1180
MunI CAATTG 1 cut(s) 767
MvaI CCWGG 4 cut(s) 344, 777, 918, 1180
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 5 cut(s) 11, 126, 508, 1181, 1208
NciI CCSGG 2 cut(s) 1098, 1151
NdeII GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
NlaIII CATG 5 cut(s) 226, 476, 624, 643, 882
NlaIV GGNNCC 4 cut(s) 261, 387, 868, 1154
NmuCI GTSAC 3 cut(s) 373, 779, 1063
OliI CACNNNNGTG 2 cut(s) 24, 314
PaeR7I CTCGAG 1 cut(s) 662
PagI TCATGA 1 cut(s) 222
PaqCI CACCTGC 3 cut(s) 82, 412, 914
PciSI GCTCTTC 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 836
PfeI GAWTC 4 cut(s) 520, 772, 883, 921
PflFI GACNNNGTC 1 cut(s) 657
PflMI CCANNNNNTGG 2 cut(s) 343, 879
PfoI TCCNGGA 1 cut(s) 1149
PleI GAGTC 3 cut(s) 191, 278, 1032
PpsI GAGTC 3 cut(s) 191, 278, 1032
PpuMI RGGWCCY 1 cut(s) 1153
Psp124BI GAGCTC 1 cut(s) 504
Psp5II RGGWCCY 1 cut(s) 1153
Psp6I CCWGG 4 cut(s) 342, 775, 916, 1178
PspGI CCWGG 4 cut(s) 342, 775, 916, 1178
PspN4I GGNNCC 4 cut(s) 261, 387, 868, 1154
PspPI GGNCC 4 cut(s) 385, 938, 1153, 1182
PspPPI RGGWCCY 1 cut(s) 1153
PsuI RGATCY 2 cut(s) 568, 1146
PsyI GACNNNGTC 1 cut(s) 657
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 2 cut(s) 551, 734
RsaNI GTAC 2 cut(s) 550, 733
RseI CAYNNNNRTG 3 cut(s) 24, 221, 314
SacI GAGCTC 1 cut(s) 504
SapI GCTCTTC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 612
Sau3AI GATC 8 cut(s) 134, 406, 490, 553, 568, 718, 1011, 1146
Sau96I GGNCC 4 cut(s) 385, 938, 1153, 1182
SchI GAGTC 3 cut(s) 191, 278, 1033
ScrFI CCNGG 6 cut(s) 344, 777, 918, 1098, 1151, 1180
SduI GDGCHC 3 cut(s) 21, 218, 504
SfaNI GCATC 5 cut(s) 149, 594, 678, 697, 730
Sfr274I CTCGAG 1 cut(s) 662
SinI GGWCC 2 cut(s) 385, 1153
SlaI CTCGAG 1 cut(s) 662
SmiMI CAYNNNNRTG 3 cut(s) 24, 221, 314
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
Sse9I AATT 5 cut(s) 614, 627, 767, 926, 989
SsiI CCGC 7 cut(s) 75, 98, 194, 257, 263, 378, 644
SspI AATATT 1 cut(s) 836
SstI GAGCTC 1 cut(s) 504
StyD4I CCNGG 6 cut(s) 342, 775, 916, 1096, 1149, 1178
TaaI ACNGT 2 cut(s) 316, 659
TaiI ACGT 1 cut(s) 47
TaqI TCGA 4 cut(s) 405, 489, 663, 705
TasI AATT 5 cut(s) 614, 627, 767, 926, 989
TauI GCSGC 2 cut(s) 78, 265
TfiI GAWTC 4 cut(s) 520, 772, 883, 921
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 2 cut(s) 21, 750
TseFI GTSAC 3 cut(s) 373, 779, 1063
Tsp45I GTSAC 3 cut(s) 373, 779, 1063
TspDTI ATGAA 2 cut(s) 211, 833
TspGWI ACGGA 1 cut(s) 1125
TspRI CASTG 2 cut(s) 21, 750
Tth111I GACNNNGTC 1 cut(s) 657
Van91I CCANNNNNTGG 2 cut(s) 343, 879
VneI GTGCAC 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 385, 1153
XagI CCTNNNNNAGG 2 cut(s) 270, 892
XapI RAATTY 3 cut(s) 614, 627, 926
XhoI CTCGAG 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 849
XmnI GAANNNNTTC 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.