Rorug07G0136800

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001305
Physical Location & Seq
Forward (+)
10797987 .. 10800767
2781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0136800.1

Sequence Viewer

Length: 1023 bp
ATGGAGGAAGGTGCAAGAGTATATGTACCCGTATCGATTGCGGAGGGTAAAGTTTCAAGGAGATTTGATTGCATACCAAGTGAGACATTGTACCCAAATGCGGATGAGATTGACTACTTGCAAAGGCTTGTCAAGTACAAGGATTCTGCTATAATAGTGCTGAACAAGCCCCCAAAATTGCCAGTCAAGGGTAATGTGCCAGTCCATAACAGCATGGATGCATTGGCAGCAGCAGCATTATCTTATGATTATGATGAAGGTCCTAAATTGGTTCATCGGATTGACAGAGAGAGCAGTGGCCTCCTTTTAATGGGAAGAACAAAAGACAGTGTTTCTCTTCTGCATTCGTTATACACTGACATCAACATTGGGAAATCATCATGCAGGTCTTGGAATGATGCATGTAGAACAAAATGTCAGAGGTACTGGGCATTGGTTATAGGGTCGCCCAAGGAAAAGGAAGGCTTAATTTGTGCTCCTCTCATGAAGGTGCTTCTTGACGATGGGAAGACAGAGAGGGTCATCTTGGCTCATCAATCAGGATTAGAAGCTTCCCAACCGGCTCTAACTCAATATCGGGTGCTTGGTCCTACGATAAATGGATGCTCATGGATTGAATTACAACCACTCACTAGCCGAAAGCATCAGCTCCGTGTTCATTGTGCTGAAGCTCTAGGCACGCCAATTGTGGGTGACTATAAGTATGGGTGGTTTGTTCACCGGAGATGGAAACAAATGCCTCAAGTCGATATTGAGCCGGCAACTGGGCTACAATACAAGTTGCGGAGGCCAGCGGGTCTGGATGTACAGAAGGGAAGTGTTCTGTCAAAAGTCCCTCTTTTGCATCTACACTGTAGAGAGCTCGTACTCCCCAATATTGCTAAGTTCCTTGTTTTATTGGATCAGAAGTCAGACAACCGGCACCCTGTAGACAGTTTGAAGCCAGATATCCTTCGGTTTGTGGCATCGATGCCAACCCACATGAAACTTAGTTGGAATCTCATGTCATCTTATTTGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

340

Amino Acids

38.26

Weight (kDa)

9.13

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 51 - 222 2.8e-21 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 375
AccB1I GGYRCC 1 cut(s) 921
AccI GTMKAC 1 cut(s) 930
AciI CCGC 4 cut(s) 41, 101, 784, 794
AclWI GGATC 1 cut(s) 909
AcuI CTGAAG 1 cut(s) 687
AfaI GTAC 6 cut(s) 27, 92, 137, 425, 807, 867
AfiI CCNNNNNNNGG 5 cut(s) 100, 188, 310, 689, 764
AgsI TTSAA 3 cut(s) 57, 617, 940
AluBI AGCT 4 cut(s) 551, 649, 671, 862
AluI AGCT 4 cut(s) 551, 649, 671, 862
Alw21I GWGCWC 2 cut(s) 478, 864
Alw26I GTCTC 1 cut(s) 77
AlwI GGATC 1 cut(s) 909
AoxI GGCC 2 cut(s) 298, 788
ApeKI GCWGC 3 cut(s) 227, 230, 233
AspS9I GGNCC 2 cut(s) 260, 587
AsuHPI GGTGA 2 cut(s) 704, 710
AvaII GGWCC 2 cut(s) 260, 587
BanI GGYRCC 1 cut(s) 921
BanII GRGCYC 1 cut(s) 864
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 2 cut(s) 478, 864
BbvI GCAGC 3 cut(s) 239, 242, 245
BccI CCATC 2 cut(s) 497, 720
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 2 cut(s) 633, 674
BfmI CTRYAG 2 cut(s) 853, 927
BfuAI ACCTGC 1 cut(s) 375
BisI GCNGC 3 cut(s) 228, 231, 234
BlsI GCNGC 3 cut(s) 229, 232, 235
Bme18I GGWCC 2 cut(s) 260, 587
BmgT120I GGNCC 2 cut(s) 260, 587
BmiI GGNNCC 1 cut(s) 923
BmrI ACTGGG 2 cut(s) 436, 774
BmsI GCATC 7 cut(s) 208, 388, 593, 652, 853, 960, 974
BmuI ACTGGG 2 cut(s) 436, 774
BpiI GAAGAC 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 726
Bsa29I ATCGAT 2 cut(s) 35, 968
BsaJI CCNNGG 1 cut(s) 450
BsaWI WCCGGW 1 cut(s) 720
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 188, 310, 689, 764
Bse118I RCCGGY 3 cut(s) 559, 757, 918
Bse1I ACTGG 4 cut(s) 182, 200, 431, 769
BseCI ATCGAT 2 cut(s) 35, 968
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 4 cut(s) 109, 223, 608, 808
BseLI CCNNNNNNNGG 5 cut(s) 100, 188, 310, 689, 764
BseNI ACTGG 4 cut(s) 182, 200, 431, 769
BseRI GAGGAG 1 cut(s) 468
BseXI GCAGC 3 cut(s) 239, 242, 245
BshFI GGCC 2 cut(s) 300, 790
BshNI GGYRCC 1 cut(s) 921
BshVI ATCGAT 2 cut(s) 35, 968
BsiHKAI GWGCWC 2 cut(s) 478, 864
BsiSI CCGG 4 cut(s) 560, 721, 758, 919
BslFI GGGAC 1 cut(s) 818
BslI CCNNNNNNNGG 5 cut(s) 100, 188, 310, 689, 764
BsmAI GTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 818
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 2 cut(s) 300, 790
Bsp1286I GDGCHC 2 cut(s) 478, 864
Bsp1407I TGTACA 1 cut(s) 805
Bsp143I GATC 1 cut(s) 901
BspACI CCGC 4 cut(s) 41, 101, 784, 794
BspANI GGCC 2 cut(s) 300, 790
BspDI ATCGAT 2 cut(s) 35, 968
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 1 cut(s) 923
BspMI ACCTGC 1 cut(s) 375
BspPI GGATC 1 cut(s) 909
BspT107I GGYRCC 1 cut(s) 921
BsrFI RCCGGY 3 cut(s) 559, 757, 918
BsrGI TGTACA 1 cut(s) 805
BsrI ACTGG 4 cut(s) 182, 200, 431, 769
BssAI RCCGGY 3 cut(s) 559, 757, 918
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 1 cut(s) 901
BssT1I CCWWGG 1 cut(s) 450
Bst4CI ACNGT 3 cut(s) 329, 854, 935
Bst6I CTCTTC 1 cut(s) 342
BstAUI TGTACA 1 cut(s) 805
BstC8I GCNNGC 3 cut(s) 680, 759, 792
BstDEI CTNAG 2 cut(s) 882, 989
BstF5I GGATG 4 cut(s) 109, 223, 608, 808
BstKTI GATC 1 cut(s) 904
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 1 cut(s) 901
BstMWI GCNNNNNNNGC 3 cut(s) 166, 227, 233
BstNSI RCATGY 1 cut(s) 405
BstSFI CTRYAG 2 cut(s) 853, 927
BstV1I GCAGC 3 cut(s) 239, 242, 245
BstV2I GAAGAC 1 cut(s) 515
Bsu15I ATCGAT 2 cut(s) 35, 968
BsuRI GGCC 2 cut(s) 300, 790
BsuTUI ATCGAT 2 cut(s) 35, 968
BtsCI GGATG 4 cut(s) 109, 223, 608, 808
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 4 cut(s) 301, 334, 354, 850
BveI ACCTGC 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 680, 759, 792
CciI TCATGA 1 cut(s) 483
Cfr10I RCCGGY 3 cut(s) 559, 757, 918
Cfr13I GGNCC 2 cut(s) 260, 587
ClaI ATCGAT 2 cut(s) 35, 968
Csp6I GTAC 6 cut(s) 26, 91, 136, 424, 806, 866
CviAII CATG 7 cut(s) 214, 381, 402, 484, 609, 982, 1003
CviQI GTAC 6 cut(s) 26, 91, 136, 424, 806, 866
DdeI CTNAG 2 cut(s) 882, 989
DpnI GATC 1 cut(s) 903
DpnII GATC 1 cut(s) 901
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Ecl136II GAGCTC 1 cut(s) 862
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 864
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 2 cut(s) 260, 587
Eco53kI GAGCTC 1 cut(s) 862
Eco57I CTGAAG 1 cut(s) 687
EcoICRI GAGCTC 1 cut(s) 862
EcoO109I RGGNCCY 1 cut(s) 260
EcoRV GATATC 1 cut(s) 949
EcoT14I CCWWGG 1 cut(s) 450
EcoT22I ATGCAT 2 cut(s) 223, 403
EcoT38I GRGCYC 1 cut(s) 864
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 7 cut(s) 217, 384, 405, 487, 612, 985, 1006
FalI AAGNNNNNCTT 4 cut(s) 449, 481, 822, 854
FaqI GGGAC 1 cut(s) 818
FatI CATG 7 cut(s) 213, 380, 401, 483, 608, 981, 1002
FauI CCCGC 1 cut(s) 787
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 3 cut(s) 228, 231, 234
FokI GGATG 4 cut(s) 116, 230, 615, 815
FriOI GRGCYC 1 cut(s) 864
Fsp4HI GCNGC 3 cut(s) 228, 231, 234
FspBI CTAG 2 cut(s) 633, 674
GluI GCNGC 3 cut(s) 228, 231, 234
HaeIII GGCC 2 cut(s) 300, 790
HapII CCGG 4 cut(s) 560, 721, 758, 919
Hin1II CATG 7 cut(s) 217, 384, 405, 487, 612, 985, 1006
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 2 cut(s) 143, 997
HpaII CCGG 4 cut(s) 560, 721, 758, 919
HphI GGTGA 2 cut(s) 704, 710
Hpy166II GTNNAC 2 cut(s) 718, 931
Hpy188I TCNGA 4 cut(s) 279, 420, 906, 913
Hpy188III TCNNGA 4 cut(s) 484, 497, 540, 800
Hpy8I GTNNAC 2 cut(s) 718, 931
HpyAV CCTTC 5 cut(s) 251, 455, 481, 805, 962
HpyCH4III ACNGT 3 cut(s) 329, 854, 935
HpyCH4V TGCA 8 cut(s) 14, 72, 121, 221, 343, 384, 401, 844
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 227, 233
HpyF3I CTNAG 2 cut(s) 882, 989
Hsp92II CATG 7 cut(s) 217, 384, 405, 487, 612, 985, 1006
KroI GCCGGC 1 cut(s) 757
KroNI GCCGGC 1 cut(s) 759
Kzo9I GATC 1 cut(s) 901
LmnI GCTCC 2 cut(s) 481, 654
Lsp1109I GCAGC 3 cut(s) 239, 242, 245
LweI GCATC 7 cut(s) 208, 388, 593, 652, 853, 960, 974
MaeI CTAG 2 cut(s) 633, 674
MaeIII GTNAC 1 cut(s) 692
MalI GATC 1 cut(s) 903
MboI GATC 1 cut(s) 901
MboII GAAGA 3 cut(s) 327, 329, 520
MfeI CAATTG 1 cut(s) 684
MhlI GDGCHC 2 cut(s) 478, 864
MluCI AATT 5 cut(s) 176, 266, 468, 617, 684
MmeI TCCRAC 1 cut(s) 974
MnlI CCTC 8 cut(s) 37, 311, 414, 489, 510, 750, 780, 846
Mph1103I ATGCAT 2 cut(s) 223, 403
MroNI GCCGGC 1 cut(s) 757
MseI TTAA 2 cut(s) 308, 467
MslI CAYNNNNRTG 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 794
MspI CCGG 4 cut(s) 560, 721, 758, 919
MunI CAATTG 1 cut(s) 684
Mva1269I GAATGC 1 cut(s) 343
MwoI GCNNNNNNNGC 3 cut(s) 166, 227, 233
NaeI GCCGGC 1 cut(s) 759
NdeII GATC 1 cut(s) 901
NgoMIV GCCGGC 1 cut(s) 757
NlaIII CATG 7 cut(s) 217, 384, 405, 487, 612, 985, 1006
NlaIV GGNNCC 1 cut(s) 923
NmuCI GTSAC 1 cut(s) 692
NsiI ATGCAT 2 cut(s) 223, 403
NspI RCATGY 1 cut(s) 405
PagI TCATGA 1 cut(s) 483
PctI GAATGC 1 cut(s) 343
PdiI GCCGGC 1 cut(s) 759
PfeI GAWTC 2 cut(s) 143, 997
PkrI GCNGC 3 cut(s) 229, 232, 235
PpuMI RGGWCCY 1 cut(s) 260
Psp124BI GAGCTC 1 cut(s) 864
Psp5II RGGWCCY 1 cut(s) 260
PspN4I GGNNCC 1 cut(s) 923
PspPI GGNCC 2 cut(s) 260, 587
PspPPI RGGWCCY 1 cut(s) 260
RsaI GTAC 6 cut(s) 27, 92, 137, 425, 807, 867
RsaNI GTAC 6 cut(s) 26, 91, 136, 424, 806, 866
RseI CAYNNNNRTG 1 cut(s) 488
SacI GAGCTC 1 cut(s) 864
SaqAI TTAA 2 cut(s) 308, 467
SatI GCNGC 3 cut(s) 228, 231, 234
Sau3AI GATC 1 cut(s) 901
Sau96I GGNCC 2 cut(s) 260, 587
SduI GDGCHC 2 cut(s) 478, 864
SetI ASST 9 cut(s) 13, 262, 389, 425, 492, 553, 651, 673, 864
SfaNI GCATC 7 cut(s) 208, 388, 593, 652, 853, 960, 974
SfcI CTRYAG 2 cut(s) 853, 927
SinI GGWCC 2 cut(s) 260, 587
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 741
SmoI CTYRAG 1 cut(s) 741
Sse9I AATT 5 cut(s) 176, 266, 468, 617, 684
SsiI CCGC 4 cut(s) 41, 101, 784, 794
SspI AATATT 1 cut(s) 877
SspMI CTAG 2 cut(s) 633, 674
SstI GAGCTC 1 cut(s) 864
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 3 cut(s) 329, 854, 935
TaqI TCGA 3 cut(s) 35, 747, 968
TasI AATT 5 cut(s) 176, 266, 468, 617, 684
TatI WGTACW 2 cut(s) 135, 805
TfiI GAWTC 2 cut(s) 143, 997
Tru1I TTAA 2 cut(s) 308, 467
Tru9I TTAA 2 cut(s) 308, 467
TscAI CASTG 4 cut(s) 301, 334, 361, 857
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 3 cut(s) 227, 230, 233
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 5 cut(s) 263, 270, 500, 647, 998
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 4 cut(s) 301, 334, 361, 857
VpaK11BI GGWCC 2 cut(s) 260, 587
XceI RCATGY 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 2 cut(s) 633, 674
Zsp2I ATGCAT 2 cut(s) 223, 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.