Rh4BG303000

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001305
Physical Location & Seq
Forward (+)
47380354 .. 47384165
3812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG303000.1

Sequence Viewer

Length: 2421 bp
ATGGAGTGCAATAGAGATGAGGCCACCAGGGCGAAAGAGATTGCGGAGAAGAAGTTTGCGGCAAGGGATGTAATGGGAGCCAAGAAATTTGCTTTGAAGGCTCAAAATTTGTTTCCGGGGCTGGAGGGTATGCCCCAAATGTTGGCAACACTCGATGTACATGTTGCTGCAGAGAACAGAGTAAATGGGGAAGTAGATTGGTATGGGGTACTTGGTCTCGAGCCGAGAGCAGATGACGAGGCGGTGAGGAAACAGTATAGGAAACTAGCTCTTATGCTTCACCCCGATAAGAACAAGTCTGTCGGGGCTGATGGAGCATTTAAGCTACTTTCCGAGGCATGGAGTTTGTTGTCTGATAAAGCTAAGAGGGGAGCGTATGACCAGAGGAGGAAGGCTTCGACTTCACAGGCGGCCCCTCCTGGAGCCAATGGGTTCTACAATTTCACAAAAAGTTCAACGTCCAGTACAAAGCCTCCAAAGAGTACTTCAAGGGCAGCTCGCTCTTCAGCTCCTGGTTCAAATCTTAAACCGAAACCCAGTACCTTTTGGACCGTCTGTCACAAGTGCAGGATGCAGTATGAGTACATGAGAGTTTATCTTAATCATAACCTTCTATGCCCGAATTGCCATGAAGCATTTTTTGCAGTGGAAATAACTCCTCCACCTACAGGTGCCTCCAAGTCATCCACCTCATGGAATCCATCACATCAGCGACAGAATTCGAATCATGAAAAAAGCACATATAATACAGGAAGAAGTAGAGATATAGGAGGGCCTTCAGGATTCACTGAATCATATCACCAAAACAACTTCCAGTGGGGTCCATTCTCCAAAGCATCTGGTGCTTCTTCGGCTGCCCAAGCTGCAAGCGTGGTTCAACAGGCATACGAGAAAGCAAAGAGAGAGCGCGAGGAAGCTCAAGCAGCAACAAAAAGAGAGGAGGCCTTGCGGAGGAAGCATCAAGCCTCCTCCAAGAAGGCGAGTGGCACTTCATCTAATGCAGCAAAGAGAAGAAGAGGAATGGATGATGTTGGTGGTAGCATCAAGCCTCCAAGGGACGTTACGAATCAAATGGGTGGGGTAGCTGGAGCTGTAAACTTTTTTGGATCTAGACAGCCTAATTTTGAGTCAGGTAGGGTCAATGGAACCACCAAGTCCAGTATCACAAGGGATTTCTCCGTGCTTGAAGTTCATAATTTACTGGCGGACAAGGCTAGGAAGGAAATTCGCAGGAAACTGAATGAACAGAAATCTGAAACTGCAGTCAAACAGGTGGTAAATGGGAATGAGACAGAGAAGTCTTTGAAAAACGTCGATGCACGGCGTGATCAGAACAAGTCTGGTGAGTCAGTTGATAGAAAGAATGGAGCTAGTGAGAGGAAACATTCTGTCACTTCTAGTGCCATAGCAGATGCAGAAACCTTGCAGTCAATGACAATAACTGTTCCAGATTCAGATTTTCATGATTTTGACAGGGACCGAACTGAAGAGTCTTTTGGAGAAAATCAGGTCTGGGCCGCATATGATGGCGATGATGGGATGCCTCGATATTATGCCATAATTCATAGTGTGATATCTTTAAACCCATTCAAAATGCGGATCAGTTGGCTTAACTCCAAAACAAATAGTGAATTGGGCCCTCTAAACTGGGTTGCTTCTGGTTTCACAAAAACATGTGGGGATTTCAGAGTTGGCAAGTATGAAATCAACAAATCACTCAATTCTTTTTCGCACAAAGTTCGGTGGACAAAAGGTGCACGAGGGATTATCTGTATATATCCCAAGAAGGGTGATGTTTGGGCTCTCTATAGGAATTGGTCCCCTGATTGGAATGAATTGACAGCCGACGAAGTAATACACAAGTATGATATGGTGGAAGTGGTTGAAGACTATAATGAAGAACTAGGTGTAACTGTAACTCCTCTGGTGAAAGTGGCTGGTTTCAAGTCAGTGTTCCACCGGCATTTGGATCCCAGAGAGGTTAGGAGGATTCCGAGAGAAGAGATGTTCCGATTCTCTCATCATGTTCCTTCATATTTGCATACCGGTCTAGAAGCATCAAATGCTCCAAAGGGTTGTCGGGAGCTAGATCCAGCAGCTACACCGTTAGAACTTCTTCAGGTAGTAACTGATGTGAAAGATGAAGAGATTATGGAGATAGATGAGGAGAAACTTAAGGAAGTTGATGTGATAGATGAAGATGAAGAGATTATGGAGATAGATGAGAAACCTAAGGAAGTTGATGTGATAGATGTTGATGTTGGAAATACTAATGGCATAGAGGAGATAGCAGAGAATTCCAGAACTGTTGGGGAAGAAGAGAGAGGGAGTTCAGTCACTCCGAAAATAGAAATTGAGGAGAGCCAAGAGACAAAGCAAGATGAAAGATTGGAGCATGCTTCGGAAGTTCAAGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

806

Amino Acids

90.09

Weight (kDa)

6.64

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 66 - 127 2.5e-20 DnaJ domain
Zn_ribbon_20 PF23551 182 - 216 5.7e-20 Zinc beta-ribbon domain
DUF3444 PF11926 477 - 683 6.1e-81 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1297
AccB1I GGYRCC 1 cut(s) 671
AccB7I CCANNNNNTGG 2 cut(s) 142, 693
AccII CGCG 1 cut(s) 909
AciI CCGC 8 cut(s) 44, 59, 242, 410, 949, 1205, 1518, 1597
AclWI GGATC 5 cut(s) 1114, 1607, 1964, 1977, 2084
AcsI RAATTY 5 cut(s) 86, 106, 718, 1224, 2296
AcuI CTGAAG 4 cut(s) 489, 762, 1506, 2102
AdeI CACNNNGTG 1 cut(s) 1325
AfaI GTAC 6 cut(s) 159, 210, 466, 484, 541, 584
AfiI CCNNNNNNNGG 8 cut(s) 142, 339, 668, 693, 951, 1787, 1827, 1966
AflII CTTAAG 1 cut(s) 2174
AflIII ACRYGT 2 cut(s) 160, 1673
AgeI ACCGGT 1 cut(s) 2045
AhdI GACNNNNNGTC 1 cut(s) 555
AjnI CCWGG 3 cut(s) 26, 418, 511
AjuI GAANNNNNNNTTGG 5 cut(s) 469, 501, 1479, 1511, 2397
Alw21I GWGCWC 1 cut(s) 1759
Alw26I GTCTC 3 cut(s) 221, 1283, 2363
Alw44I GTGCAC 1 cut(s) 1755
AlwI GGATC 5 cut(s) 1114, 1607, 1964, 1977, 2084
AlwNI CAGNNNCTG 1 cut(s) 512
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 6 cut(s) 21, 411, 773, 942, 1515, 1636
ApaI GGGCCC 1 cut(s) 1640
ApaLI GTGCAC 1 cut(s) 1755
ApeKI GCWGC 7 cut(s) 167, 494, 854, 863, 923, 1001, 2096
ApoI RAATTY 5 cut(s) 86, 106, 718, 1224, 2296
AsiGI ACCGGT 1 cut(s) 2045
Asp700I GAANNNNTTC 1 cut(s) 2115
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 9 cut(s) 412, 549, 773, 821, 1477, 1515, 1636, 1637, 1818
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 6 cut(s) 256, 272, 791, 1355, 1802, 1939
AsuII TTCGAA 1 cut(s) 722
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 4 cut(s) 549, 821, 1477, 1818
AxyI CCTNAGG 1 cut(s) 2232
BaeGI GKGCMC 2 cut(s) 1640, 1759
BamHI GGATCC 1 cut(s) 1969
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 2 cut(s) 1640, 1804
BauI CACGAG 1 cut(s) 1758
BbsI GAAGAC 1 cut(s) 1893
Bbv12I GWGCWC 1 cut(s) 1759
BbvI GCAGC 7 cut(s) 154, 506, 841, 850, 935, 1013, 2108
BccI CCATC 4 cut(s) 305, 709, 1520, 1529
BceAI ACGGC 1 cut(s) 1337
BcgI CGANNNNNNTGC 2 cut(s) 1721, 1755
BciT130I CCWGG 3 cut(s) 28, 420, 513
BclI TGATCA 1 cut(s) 1327
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 3 cut(s) 221, 1283, 2363
BfaI CTAG 8 cut(s) 266, 1110, 1215, 1371, 1398, 1904, 2051, 2087
BfmI CTRYAG 4 cut(s) 168, 666, 1260, 1807
BfrI CTTAAG 1 cut(s) 2174
BglI GCCNNNNNGGC 1 cut(s) 29
BmcAI AGTACT 1 cut(s) 484
Bme1390I CCNGG 4 cut(s) 28, 117, 420, 513
Bme18I GGWCC 4 cut(s) 549, 821, 1477, 1818
BmeRI GACNNNNNGTC 1 cut(s) 555
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 9 cut(s) 412, 549, 773, 821, 1477, 1515, 1636, 1637, 1818
BmrFI CCNGG 4 cut(s) 28, 117, 420, 513
BmrI ACTGGG 2 cut(s) 531, 1657
BmsI GCATC 8 cut(s) 561, 845, 967, 1050, 1306, 1402, 1530, 2066
BmuI ACTGGG 2 cut(s) 531, 1657
BpiI GAAGAC 1 cut(s) 1893
BpmI CTGGAG 3 cut(s) 143, 441, 1107
Bpu14I TTCGAA 1 cut(s) 722
BpuEI CTTGAG 1 cut(s) 903
BpuMI CCSGG 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 4 cut(s) 27, 116, 333, 1052
BsaWI WCCGGW 1 cut(s) 2045
BsaXI ACNNNNNCTCC 6 cut(s) 38, 68, 457, 487, 1903, 1933
Bsc4I CCNNNNNNNGG 8 cut(s) 142, 339, 668, 693, 951, 1787, 1827, 1966
Bse118I RCCGGY 2 cut(s) 1959, 2045
Bse1I ACTGG 6 cut(s) 462, 537, 814, 1158, 1206, 1652
Bse21I CCTNAGG 1 cut(s) 2232
BseBI CCWGG 3 cut(s) 28, 420, 513
BseDI CCNNGG 4 cut(s) 27, 116, 333, 1052
BseGI GGATG 5 cut(s) 73, 576, 683, 1030, 1545
BseLI CCNNNNNNNGG 8 cut(s) 142, 339, 668, 693, 951, 1787, 1827, 1966
BseNI ACTGG 6 cut(s) 462, 537, 814, 1158, 1206, 1652
BseRI GAGGAG 8 cut(s) 400, 648, 953, 958, 1911, 2180, 2297, 2372
BseSI GKGCMC 2 cut(s) 1640, 1759
BseXI GCAGC 7 cut(s) 154, 506, 841, 850, 935, 1013, 2108
BsgI GTGCAG 1 cut(s) 586
Bsh1236I CGCG 1 cut(s) 909
BshFI GGCC 6 cut(s) 23, 413, 775, 944, 1517, 1638
BshNI GGYRCC 1 cut(s) 671
BshTI ACCGGT 1 cut(s) 2045
BsiHKAI GWGCWC 1 cut(s) 1759
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 3 cut(s) 116, 1960, 2046
BslFI GGGAC 3 cut(s) 1070, 1490, 1804
BslI CCNNNNNNNGG 8 cut(s) 142, 339, 668, 693, 951, 1787, 1827, 1966
BsmAI GTCTC 3 cut(s) 221, 1283, 2363
BsmFI GGGAC 3 cut(s) 1070, 1490, 1804
BsnI GGCC 6 cut(s) 23, 413, 775, 944, 1517, 1638
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 722
Bsp120I GGGCCC 1 cut(s) 1636
Bsp1286I GDGCHC 3 cut(s) 1640, 1759, 1804
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
BspACI CCGC 8 cut(s) 44, 59, 242, 410, 949, 1205, 1518, 1597
BspANI GGCC 6 cut(s) 23, 413, 775, 944, 1517, 1638
BspFNI CGCG 1 cut(s) 909
BspHI TCATGA 2 cut(s) 727, 1462
BspMAI CTGCAG 2 cut(s) 172, 1264
BspPI GGATC 5 cut(s) 1114, 1607, 1964, 1977, 2084
BspQI GCTCTTC 1 cut(s) 508
BspT104I TTCGAA 1 cut(s) 722
BspT107I GGYRCC 1 cut(s) 671
BspTI CTTAAG 1 cut(s) 2174
BspTNI GGTCTC 1 cut(s) 221
BsrFI RCCGGY 2 cut(s) 1959, 2045
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 6 cut(s) 462, 537, 814, 1158, 1206, 1652
BssAI RCCGGY 2 cut(s) 1959, 2045
BssECI CCNNGG 4 cut(s) 27, 116, 333, 1052
BssMI GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
BssSI CACGAG 1 cut(s) 1758
BssT1I CCWWGG 1 cut(s) 1052
Bst2BI CACGAG 1 cut(s) 1758
Bst2UI CCWGG 3 cut(s) 28, 420, 513
Bst4CI ACNGT 6 cut(s) 255, 553, 1444, 1915, 2106, 2308
Bst6I CTCTTC 7 cut(s) 508, 1009, 1482, 1995, 2139, 2199, 2313
BstAFI CTTAAG 1 cut(s) 2174
BstAPI GCANNNNNTGC 3 cut(s) 641, 842, 2063
BstAUI TGTACA 1 cut(s) 157
BstBI TTCGAA 1 cut(s) 722
BstC8I GCNNGC 3 cut(s) 499, 868, 2397
BstDEI CTNAG 2 cut(s) 363, 2232
BstENI CCTNNNNNAGG 1 cut(s) 949
BstF5I GGATG 5 cut(s) 73, 576, 683, 1030, 1545
BstFNI CGCG 1 cut(s) 909
BstHHI GCGC 1 cut(s) 909
BstKTI GATC 5 cut(s) 1109, 1330, 1602, 1972, 2092
BstMAI GTCTC 3 cut(s) 221, 1283, 2363
BstMBI GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
BstNI CCWGG 3 cut(s) 28, 420, 513
BstNSI RCATGY 3 cut(s) 164, 1677, 2399
BstSCI CCNGG 4 cut(s) 26, 115, 418, 511
BstSFI CTRYAG 4 cut(s) 168, 666, 1260, 1807
BstSLI GKGCMC 2 cut(s) 1640, 1759
BstUI CGCG 1 cut(s) 909
BstV1I GCAGC 7 cut(s) 154, 506, 841, 850, 935, 1013, 2108
BstV2I GAAGAC 1 cut(s) 1893
BstX2I RGATCY 3 cut(s) 1106, 1969, 2089
BstYI RGATCY 3 cut(s) 1106, 1969, 2089
Bsu36I CCTNAGG 1 cut(s) 2232
BsuRI GGCC 6 cut(s) 23, 413, 775, 944, 1517, 1638
BtgZI GCGATG 1 cut(s) 1545
BtsCI GGATG 5 cut(s) 73, 576, 683, 1030, 1545
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 4 cut(s) 651, 786, 821, 1956
Cac8I GCNNGC 3 cut(s) 499, 868, 2397
CaiI CAGNNNCTG 1 cut(s) 512
CciI TCATGA 2 cut(s) 727, 1462
CfoI GCGC 1 cut(s) 909
Cfr10I RCCGGY 2 cut(s) 1959, 2045
Cfr13I GGNCC 9 cut(s) 412, 549, 773, 821, 1477, 1515, 1636, 1637, 1818
Csp6I GTAC 6 cut(s) 158, 209, 465, 483, 540, 583
CspAI ACCGGT 1 cut(s) 2045
CviQI GTAC 6 cut(s) 158, 209, 465, 483, 540, 583
DdeI CTNAG 2 cut(s) 363, 2232
DpnI GATC 5 cut(s) 1108, 1329, 1601, 1971, 2091
DpnII GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
DraI TTTAAA 1 cut(s) 1581
DraIII CACNNNGTG 1 cut(s) 1325
DrdI GACNNNNNNGTC 1 cut(s) 1297
DriI GACNNNNNGTC 1 cut(s) 555
DseDI GACNNNNNNGTC 1 cut(s) 1297
Eam1104I CTCTTC 7 cut(s) 508, 1009, 1482, 1995, 2139, 2199, 2313
Eam1105I GACNNNNNGTC 1 cut(s) 555
EarI CTCTTC 7 cut(s) 508, 1009, 1482, 1995, 2139, 2199, 2313
EciI GGCGGA 1 cut(s) 1220
Eco130I CCWWGG 1 cut(s) 1052
Eco147I AGGCCT 1 cut(s) 944
Eco24I GRGCYC 2 cut(s) 1640, 1804
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 1575
Eco47I GGWCC 4 cut(s) 549, 821, 1477, 1818
Eco57I CTGAAG 4 cut(s) 489, 762, 1506, 2102
Eco81I CCTNAGG 1 cut(s) 2232
Eco88I CYCGRG 1 cut(s) 218
EcoNI CCTNNNNNAGG 1 cut(s) 949
EcoO109I RGGNCCY 2 cut(s) 773, 1637
EcoRI GAATTC 2 cut(s) 718, 2296
EcoRII CCWGG 3 cut(s) 26, 418, 511
EcoRV GATATC 1 cut(s) 1575
EcoT14I CCWWGG 1 cut(s) 1052
EcoT38I GRGCYC 2 cut(s) 1640, 1804
ErhI CCWWGG 1 cut(s) 1052
FaqI GGGAC 3 cut(s) 1070, 1490, 1804
FauNDI CATATG 1 cut(s) 1522
FbaI TGATCA 1 cut(s) 1327
FokI GGATG 5 cut(s) 80, 583, 670, 1037, 1552
FriOI GRGCYC 2 cut(s) 1640, 1804
FspBI CTAG 8 cut(s) 266, 1110, 1215, 1371, 1398, 1904, 2051, 2087
GlaI GCGC 1 cut(s) 908
GsuI CTGGAG 3 cut(s) 143, 441, 1107
HaeIII GGCC 6 cut(s) 23, 413, 775, 944, 1517, 1638
HapII CCGG 3 cut(s) 116, 1960, 2046
HhaI GCGC 1 cut(s) 909
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HpaII CCGG 3 cut(s) 116, 1960, 2046
HphI GGTGA 6 cut(s) 256, 272, 791, 1355, 1802, 1939
Hpy166II GTNNAC 3 cut(s) 1096, 1746, 1757
Hpy188III TCNNGA 9 cut(s) 218, 728, 780, 1110, 1448, 1463, 2051, 2081, 2301
Hpy8I GTNNAC 3 cut(s) 1096, 1746, 1757
Hpy99I CGWCG 2 cut(s) 1316, 1850
HpyAV CCTTC 8 cut(s) 91, 385, 620, 786, 970, 1213, 1780, 2040
HpyCH4III ACNGT 6 cut(s) 255, 553, 1444, 1915, 2106, 2308
HpyCH4IV ACGT 3 cut(s) 458, 1059, 1311
HpyF3I CTNAG 2 cut(s) 363, 2232
HpySE526I ACGT 3 cut(s) 458, 1059, 1311
HspAI GCGC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 1327
Kzo9I GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
LguI GCTCTTC 1 cut(s) 508
Lsp1109I GCAGC 7 cut(s) 154, 506, 841, 850, 935, 1013, 2108
LweI GCATC 8 cut(s) 561, 845, 967, 1050, 1306, 1402, 1530, 2066
MaeI CTAG 8 cut(s) 266, 1110, 1215, 1371, 1398, 1904, 2051, 2087
MaeII ACGT 3 cut(s) 458, 1059, 1311
MaeIII GTNAC 7 cut(s) 557, 1060, 1390, 1909, 1915, 2125, 2335
MalI GATC 5 cut(s) 1108, 1329, 1601, 1971, 2091
MboI GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
MflI RGATCY 3 cut(s) 1106, 1969, 2089
MhlI GDGCHC 3 cut(s) 1640, 1759, 1804
MlyI GAGTC 3 cut(s) 1136, 1355, 1499
MmeI TCCRAC 1 cut(s) 2242
MroXI GAANNNNTTC 1 cut(s) 2115
MseI TTAA 6 cut(s) 321, 525, 600, 1580, 1611, 2175
MslI CAYNNNNRTG 1 cut(s) 1863
MspCI CTTAAG 1 cut(s) 2174
MspI CCGG 3 cut(s) 116, 1960, 2046
MspR9I CCNGG 4 cut(s) 28, 117, 420, 513
MvaI CCWGG 3 cut(s) 28, 420, 513
MvnI CGCG 1 cut(s) 909
NciI CCSGG 1 cut(s) 117
NdeI CATATG 1 cut(s) 1522
NdeII GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
NmeAIII GCCGAG 1 cut(s) 249
NmuCI GTSAC 3 cut(s) 557, 1390, 2335
NspI RCATGY 3 cut(s) 164, 1677, 2399
NspV TTCGAA 1 cut(s) 722
PaeI GCATGC 1 cut(s) 2399
PaeR7I CTCGAG 1 cut(s) 218
PagI TCATGA 2 cut(s) 727, 1462
PceI AGGCCT 1 cut(s) 944
PciI ACATGT 2 cut(s) 160, 1673
PciSI GCTCTTC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 2115
PfeI GAWTC 8 cut(s) 697, 724, 783, 791, 1066, 1451, 1990, 2013
PflMI CCANNNNNTGG 2 cut(s) 142, 693
PfoI TCCNGGA 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 2045
PleI GAGTC 3 cut(s) 1135, 1354, 1498
PpsI GAGTC 3 cut(s) 1135, 1354, 1498
PscI ACATGT 2 cut(s) 160, 1673
Psp6I CCWGG 3 cut(s) 26, 418, 511
PspGI CCWGG 3 cut(s) 26, 418, 511
PspOMI GGGCCC 1 cut(s) 1636
PspPI GGNCC 9 cut(s) 412, 549, 773, 821, 1477, 1515, 1636, 1637, 1818
PstI CTGCAG 2 cut(s) 172, 1264
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 3 cut(s) 1106, 1969, 2089
RsaI GTAC 6 cut(s) 159, 210, 466, 484, 541, 584
RsaNI GTAC 6 cut(s) 158, 209, 465, 483, 540, 583
RseI CAYNNNNRTG 1 cut(s) 1863
SapI GCTCTTC 1 cut(s) 508
SaqAI TTAA 6 cut(s) 321, 525, 600, 1580, 1611, 2175
Sau3AI GATC 5 cut(s) 1106, 1327, 1599, 1969, 2089
Sau96I GGNCC 9 cut(s) 412, 549, 773, 821, 1477, 1515, 1636, 1637, 1818
ScaI AGTACT 1 cut(s) 484
SchI GAGTC 3 cut(s) 1136, 1355, 1499
ScrFI CCNGG 4 cut(s) 28, 117, 420, 513
SduI GDGCHC 3 cut(s) 1640, 1759, 1804
SfaNI GCATC 8 cut(s) 561, 845, 967, 1050, 1306, 1402, 1530, 2066
SfcI CTRYAG 4 cut(s) 168, 666, 1260, 1807
Sfr274I CTCGAG 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 722
SinI GGWCC 4 cut(s) 549, 821, 1477, 1818
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 1863
SmlI CTYRAG 3 cut(s) 218, 918, 2174
SmoI CTYRAG 3 cut(s) 218, 918, 2174
SphI GCATGC 1 cut(s) 2399
SseBI AGGCCT 1 cut(s) 944
SsiI CCGC 8 cut(s) 44, 59, 242, 410, 949, 1205, 1518, 1597
SspMI CTAG 8 cut(s) 266, 1110, 1215, 1371, 1398, 1904, 2051, 2087
StuI AGGCCT 1 cut(s) 944
StyD4I CCNGG 4 cut(s) 26, 115, 418, 511
StyI CCWWGG 1 cut(s) 1052
TaaI ACNGT 6 cut(s) 255, 553, 1444, 1915, 2106, 2308
TaiI ACGT 3 cut(s) 461, 1062, 1314
TaqI TCGA 6 cut(s) 153, 219, 398, 722, 1314, 1546
TaqII GACCGA 1 cut(s) 1494
TatI WGTACW 4 cut(s) 157, 464, 482, 582
TauI GCSGC 3 cut(s) 62, 413, 1520
TfiI GAWTC 8 cut(s) 697, 724, 783, 791, 1066, 1451, 1990, 2013
Tru1I TTAA 6 cut(s) 321, 525, 600, 1580, 1611, 2175
Tru9I TTAA 6 cut(s) 321, 525, 600, 1580, 1611, 2175
TscAI CASTG 4 cut(s) 651, 793, 821, 1956
TseFI GTSAC 3 cut(s) 557, 1390, 2335
TseI GCWGC 7 cut(s) 167, 494, 854, 863, 923, 1001, 2096
Tsp45I GTSAC 3 cut(s) 557, 1390, 2335
TspGWI ACGGA 1 cut(s) 1168
TspRI CASTG 4 cut(s) 651, 793, 821, 1956
Van91I CCANNNNNTGG 2 cut(s) 142, 693
Vha464I CTTAAG 1 cut(s) 2174
VneI GTGCAC 1 cut(s) 1755
VpaK11BI GGWCC 4 cut(s) 549, 821, 1477, 1818
XagI CCTNNNNNAGG 1 cut(s) 949
XapI RAATTY 5 cut(s) 86, 106, 718, 1224, 2296
XbaI TCTAGA 2 cut(s) 1109, 2050
XceI RCATGY 3 cut(s) 164, 1677, 2399
XhoI CTCGAG 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 2115
XspI CTAG 8 cut(s) 266, 1110, 1215, 1371, 1398, 1904, 2051, 2087
ZrmI AGTACT 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.