Domain of unknown function (DUF3444)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 668 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 142, 690 |
| AccII | CGCG | 1 cut(s) | 906 |
| AciI | CCGC | 7 cut(s) | 44, 59, 242, 946, 1146, 1515, 1594 |
| AclI | AACGTT | 1 cut(s) | 1308 |
| AclWI | GGATC | 5 cut(s) | 1111, 1604, 1961, 1974, 2081 |
| AcsI | RAATTY | 5 cut(s) | 86, 106, 715, 1221, 2314 |
| AcuI | CTGAAG | 4 cut(s) | 486, 759, 1503, 2099 |
| AdeI | CACNNNGTG | 1 cut(s) | 1322 |
| AfaI | GTAC | 6 cut(s) | 159, 210, 463, 481, 538, 581 |
| AflIII | ACRYGT | 2 cut(s) | 160, 1670 |
| AgeI | ACCGGT | 1 cut(s) | 2042 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 552 |
| AjnI | CCWGG | 3 cut(s) | 415, 508, 1503 |
| AjuI | GAANNNNNNNTTGG | 5 cut(s) | 466, 498, 1476, 1508, 2415 |
| Alw21I | GWGCWC | 1 cut(s) | 1756 |
| Alw26I | GTCTC | 2 cut(s) | 221, 1280 |
| Alw44I | GTGCAC | 1 cut(s) | 1752 |
| AlwI | GGATC | 5 cut(s) | 1111, 1604, 1961, 1974, 2081 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 509 |
| Ama87I | CYCGRG | 1 cut(s) | 218 |
| AoxI | GGCC | 4 cut(s) | 21, 939, 1512, 1633 |
| ApaI | GGGCCC | 1 cut(s) | 1637 |
| ApaLI | GTGCAC | 1 cut(s) | 1752 |
| ApeKI | GCWGC | 8 cut(s) | 167, 491, 851, 860, 920, 998, 1088, 2093 |
| ApoI | RAATTY | 5 cut(s) | 86, 106, 715, 1221, 2314 |
| AsiGI | ACCGGT | 1 cut(s) | 2042 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2112 |
| AspLEI | GCGC | 1 cut(s) | 906 |
| AspS9I | GGNCC | 8 cut(s) | 546, 770, 818, 1474, 1512, 1633, 1634, 1815 |
| AsuC2I | CCSGG | 1 cut(s) | 117 |
| AsuHPI | GGTGA | 6 cut(s) | 256, 272, 788, 1352, 1799, 1936 |
| AsuII | TTCGAA | 1 cut(s) | 719 |
| AvaI | CYCGRG | 1 cut(s) | 218 |
| AvaII | GGWCC | 5 cut(s) | 546, 770, 818, 1474, 1815 |
| AxyI | CCTNAGG | 2 cut(s) | 2169, 2250 |
| BaeGI | GKGCMC | 2 cut(s) | 1637, 1756 |
| BamHI | GGATCC | 1 cut(s) | 1966 |
| BanI | GGYRCC | 1 cut(s) | 668 |
| BanII | GRGCYC | 2 cut(s) | 1637, 1801 |
| BauI | CACGAG | 1 cut(s) | 1755 |
| BbsI | GAAGAC | 1 cut(s) | 1890 |
| Bbv12I | GWGCWC | 1 cut(s) | 1756 |
| BbvI | GCAGC | 8 cut(s) | 154, 503, 838, 847, 932, 1010, 1075, 2105 |
| BccI | CCATC | 6 cut(s) | 32, 305, 706, 1517, 1526, 2149 |
| BceAI | ACGGC | 2 cut(s) | 1334, 1895 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1718, 1752 |
| BciT130I | CCWGG | 3 cut(s) | 417, 510, 1505 |
| BclI | TGATCA | 1 cut(s) | 1324 |
| BcnI | CCSGG | 1 cut(s) | 117 |
| BcoDI | GTCTC | 2 cut(s) | 221, 1280 |
| BfaI | CTAG | 7 cut(s) | 266, 1107, 1212, 1368, 1395, 1901, 2084 |
| BfmI | CTRYAG | 4 cut(s) | 168, 663, 1257, 1804 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 29 |
| BmcAI | AGTACT | 1 cut(s) | 481 |
| Bme1390I | CCNGG | 4 cut(s) | 117, 417, 510, 1505 |
| Bme18I | GGWCC | 5 cut(s) | 546, 770, 818, 1474, 1815 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 552 |
| BmeT110I | CYCGRG | 1 cut(s) | 218 |
| BmgT120I | GGNCC | 8 cut(s) | 546, 770, 818, 1474, 1512, 1633, 1634, 1815 |
| BmiI | GGNNCC | 9 cut(s) | 79, 421, 670, 819, 1144, 1475, 1635, 1817, 1968 |
| BmrFI | CCNGG | 4 cut(s) | 117, 417, 510, 1505 |
| BmrI | ACTGGG | 2 cut(s) | 528, 1654 |
| BmsI | GCATC | 7 cut(s) | 558, 964, 1047, 1303, 1399, 1527, 2063 |
| BmuI | ACTGGG | 2 cut(s) | 528, 1654 |
| BpiI | GAAGAC | 1 cut(s) | 1890 |
| BpmI | CTGGAG | 3 cut(s) | 143, 438, 1104 |
| Bpu14I | TTCGAA | 1 cut(s) | 719 |
| BpuEI | CTTGAG | 1 cut(s) | 900 |
| BpuMI | CCSGG | 1 cut(s) | 117 |
| BsaI | GGTCTC | 1 cut(s) | 221 |
| BsaJI | CCNNGG | 3 cut(s) | 116, 333, 1049 |
| BsaWI | WCCGGW | 1 cut(s) | 2042 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 38, 68 |
| Bse118I | RCCGGY | 2 cut(s) | 1956, 2042 |
| Bse1I | ACTGG | 7 cut(s) | 459, 534, 811, 1155, 1203, 1649, 2212 |
| Bse21I | CCTNAGG | 2 cut(s) | 2169, 2250 |
| BseBI | CCWGG | 3 cut(s) | 417, 510, 1505 |
| BseDI | CCNNGG | 3 cut(s) | 116, 333, 1049 |
| BseGI | GGATG | 5 cut(s) | 73, 573, 1027, 1542, 2164 |
| BseNI | ACTGG | 7 cut(s) | 459, 534, 811, 1155, 1203, 1649, 2212 |
| BseRI | GAGGAG | 7 cut(s) | 400, 645, 950, 955, 1908, 2315, 2390 |
| BseSI | GKGCMC | 2 cut(s) | 1637, 1756 |
| BseXI | GCAGC | 8 cut(s) | 154, 503, 838, 847, 932, 1010, 1075, 2105 |
| BsgI | GTGCAG | 1 cut(s) | 583 |
| Bsh1236I | CGCG | 1 cut(s) | 906 |
| BshFI | GGCC | 4 cut(s) | 23, 941, 1514, 1635 |
| BshNI | GGYRCC | 1 cut(s) | 668 |
| BshTI | ACCGGT | 1 cut(s) | 2042 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1756 |
| BsiHKCI | CYCGRG | 1 cut(s) | 218 |
| BsiSI | CCGG | 3 cut(s) | 116, 1957, 2043 |
| BslFI | GGGAC | 3 cut(s) | 1067, 1487, 1801 |
| BsmAI | GTCTC | 2 cut(s) | 221, 1280 |
| BsmFI | GGGAC | 3 cut(s) | 1067, 1487, 1801 |
| BsnI | GGCC | 4 cut(s) | 23, 941, 1514, 1635 |
| Bso31I | GGTCTC | 1 cut(s) | 221 |
| BsoBI | CYCGRG | 1 cut(s) | 218 |
| Bsp119I | TTCGAA | 1 cut(s) | 719 |
| Bsp120I | GGGCCC | 1 cut(s) | 1633 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1637, 1756, 1801 |
| Bsp1407I | TGTACA | 1 cut(s) | 157 |
| Bsp143I | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| BspACI | CCGC | 7 cut(s) | 44, 59, 242, 946, 1146, 1515, 1594 |
| BspANI | GGCC | 4 cut(s) | 23, 941, 1514, 1635 |
| BspFNI | CGCG | 1 cut(s) | 906 |
| BspHI | TCATGA | 2 cut(s) | 724, 1459 |
| BspLI | GGNNCC | 9 cut(s) | 79, 421, 670, 819, 1144, 1475, 1635, 1817, 1968 |
| BspMAI | CTGCAG | 2 cut(s) | 172, 1261 |
| BspPI | GGATC | 5 cut(s) | 1111, 1604, 1961, 1974, 2081 |
| BspQI | GCTCTTC | 1 cut(s) | 505 |
| BspT104I | TTCGAA | 1 cut(s) | 719 |
| BspT107I | GGYRCC | 1 cut(s) | 668 |
| BspTNI | GGTCTC | 1 cut(s) | 221 |
| BsrFI | RCCGGY | 2 cut(s) | 1956, 2042 |
| BsrGI | TGTACA | 1 cut(s) | 157 |
| BsrI | ACTGG | 7 cut(s) | 459, 534, 811, 1155, 1203, 1649, 2212 |
| BssAI | RCCGGY | 2 cut(s) | 1956, 2042 |
| BssECI | CCNNGG | 3 cut(s) | 116, 333, 1049 |
| BssMI | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| BssSI | CACGAG | 1 cut(s) | 1755 |
| BssT1I | CCWWGG | 1 cut(s) | 1049 |
| Bst2BI | CACGAG | 1 cut(s) | 1755 |
| Bst2UI | CCWGG | 3 cut(s) | 417, 510, 1505 |
| Bst4CI | ACNGT | 5 cut(s) | 255, 550, 1441, 2103, 2326 |
| Bst6I | CTCTTC | 7 cut(s) | 505, 1006, 1479, 1992, 2136, 2217, 2331 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 638, 2060 |
| BstAUI | TGTACA | 1 cut(s) | 157 |
| BstBI | TTCGAA | 1 cut(s) | 719 |
| BstC8I | GCNNGC | 3 cut(s) | 496, 865, 2415 |
| BstDEI | CTNAG | 3 cut(s) | 363, 2169, 2250 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 946 |
| BstF5I | GGATG | 5 cut(s) | 73, 573, 1027, 1542, 2164 |
| BstFNI | CGCG | 1 cut(s) | 906 |
| BstHHI | GCGC | 1 cut(s) | 906 |
| BstKTI | GATC | 5 cut(s) | 1106, 1327, 1599, 1969, 2089 |
| BstMAI | GTCTC | 2 cut(s) | 221, 1280 |
| BstMBI | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| BstNI | CCWGG | 3 cut(s) | 417, 510, 1505 |
| BstNSI | RCATGY | 3 cut(s) | 164, 1674, 2417 |
| BstSCI | CCNGG | 4 cut(s) | 115, 415, 508, 1503 |
| BstSFI | CTRYAG | 4 cut(s) | 168, 663, 1257, 1804 |
| BstSLI | GKGCMC | 2 cut(s) | 1637, 1756 |
| BstUI | CGCG | 1 cut(s) | 906 |
| BstV1I | GCAGC | 8 cut(s) | 154, 503, 838, 847, 932, 1010, 1075, 2105 |
| BstV2I | GAAGAC | 1 cut(s) | 1890 |
| BstX2I | RGATCY | 3 cut(s) | 1103, 1966, 2086 |
| BstYI | RGATCY | 3 cut(s) | 1103, 1966, 2086 |
| Bsu36I | CCTNAGG | 2 cut(s) | 2169, 2250 |
| BsuRI | GGCC | 4 cut(s) | 23, 941, 1514, 1635 |
| BtgZI | GCGATG | 1 cut(s) | 1542 |
| BtsCI | GGATG | 5 cut(s) | 73, 573, 1027, 1542, 2164 |
| BtsI | GCAGTG | 1 cut(s) | 648 |
| BtsIMutI | CAGTG | 5 cut(s) | 648, 783, 818, 1953, 2358 |
| Cac8I | GCNNGC | 3 cut(s) | 496, 865, 2415 |
| CaiI | CAGNNNCTG | 1 cut(s) | 509 |
| CciI | TCATGA | 2 cut(s) | 724, 1459 |
| CfoI | GCGC | 1 cut(s) | 906 |
| Cfr10I | RCCGGY | 2 cut(s) | 1956, 2042 |
| Cfr13I | GGNCC | 8 cut(s) | 546, 770, 818, 1474, 1512, 1633, 1634, 1815 |
| CsiI | ACCWGGT | 1 cut(s) | 1503 |
| Csp6I | GTAC | 6 cut(s) | 158, 209, 462, 480, 537, 580 |
| CspAI | ACCGGT | 1 cut(s) | 2042 |
| CviQI | GTAC | 6 cut(s) | 158, 209, 462, 480, 537, 580 |
| DdeI | CTNAG | 3 cut(s) | 363, 2169, 2250 |
| DpnI | GATC | 5 cut(s) | 1105, 1326, 1598, 1968, 2088 |
| DpnII | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| DraI | TTTAAA | 1 cut(s) | 1578 |
| DraIII | CACNNNGTG | 1 cut(s) | 1322 |
| DriI | GACNNNNNGTC | 1 cut(s) | 552 |
| Eam1104I | CTCTTC | 7 cut(s) | 505, 1006, 1479, 1992, 2136, 2217, 2331 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 552 |
| EarI | CTCTTC | 7 cut(s) | 505, 1006, 1479, 1992, 2136, 2217, 2331 |
| Eco130I | CCWWGG | 1 cut(s) | 1049 |
| Eco147I | AGGCCT | 1 cut(s) | 941 |
| Eco24I | GRGCYC | 2 cut(s) | 1637, 1801 |
| Eco31I | GGTCTC | 1 cut(s) | 221 |
| Eco32I | GATATC | 1 cut(s) | 1572 |
| Eco47I | GGWCC | 5 cut(s) | 546, 770, 818, 1474, 1815 |
| Eco57I | CTGAAG | 4 cut(s) | 486, 759, 1503, 2099 |
| Eco81I | CCTNAGG | 2 cut(s) | 2169, 2250 |
| Eco88I | CYCGRG | 1 cut(s) | 218 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 946 |
| EcoO109I | RGGNCCY | 2 cut(s) | 770, 1634 |
| EcoRI | GAATTC | 2 cut(s) | 715, 2314 |
| EcoRII | CCWGG | 3 cut(s) | 415, 508, 1503 |
| EcoRV | GATATC | 1 cut(s) | 1572 |
| EcoT14I | CCWWGG | 1 cut(s) | 1049 |
| EcoT38I | GRGCYC | 2 cut(s) | 1637, 1801 |
| ErhI | CCWWGG | 1 cut(s) | 1049 |
| FaqI | GGGAC | 3 cut(s) | 1067, 1487, 1801 |
| FauNDI | CATATG | 1 cut(s) | 1519 |
| FbaI | TGATCA | 1 cut(s) | 1324 |
| FokI | GGATG | 5 cut(s) | 80, 580, 1034, 1549, 2171 |
| FriOI | GRGCYC | 2 cut(s) | 1637, 1801 |
| FspBI | CTAG | 7 cut(s) | 266, 1107, 1212, 1368, 1395, 1901, 2084 |
| GlaI | GCGC | 1 cut(s) | 905 |
| GsuI | CTGGAG | 3 cut(s) | 143, 438, 1104 |
| HaeIII | GGCC | 4 cut(s) | 23, 941, 1514, 1635 |
| HapII | CCGG | 3 cut(s) | 116, 1957, 2043 |
| HhaI | GCGC | 1 cut(s) | 906 |
| Hin6I | GCGC | 1 cut(s) | 904 |
| HinP1I | GCGC | 1 cut(s) | 904 |
| HpaII | CCGG | 3 cut(s) | 116, 1957, 2043 |
| HphI | GGTGA | 6 cut(s) | 256, 272, 788, 1352, 1799, 1936 |
| Hpy166II | GTNNAC | 3 cut(s) | 684, 1743, 1754 |
| Hpy188III | TCNNGA | 8 cut(s) | 218, 725, 777, 1107, 1445, 1460, 2078, 2319 |
| Hpy8I | GTNNAC | 3 cut(s) | 684, 1743, 1754 |
| Hpy99I | CGWCG | 1 cut(s) | 1847 |
| HpyAV | CCTTC | 8 cut(s) | 91, 385, 617, 783, 967, 1210, 1777, 2037 |
| HpyCH4III | ACNGT | 5 cut(s) | 255, 550, 1441, 2103, 2326 |
| HpyCH4IV | ACGT | 3 cut(s) | 455, 1056, 1308 |
| HpyF3I | CTNAG | 3 cut(s) | 363, 2169, 2250 |
| HpySE526I | ACGT | 3 cut(s) | 455, 1056, 1308 |
| HspAI | GCGC | 1 cut(s) | 904 |
| Ksp22I | TGATCA | 1 cut(s) | 1324 |
| Kzo9I | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| LguI | GCTCTTC | 1 cut(s) | 505 |
| Lsp1109I | GCAGC | 8 cut(s) | 154, 503, 838, 847, 932, 1010, 1075, 2105 |
| LweI | GCATC | 7 cut(s) | 558, 964, 1047, 1303, 1399, 1527, 2063 |
| MabI | ACCWGGT | 1 cut(s) | 1503 |
| MaeI | CTAG | 7 cut(s) | 266, 1107, 1212, 1368, 1395, 1901, 2084 |
| MaeII | ACGT | 3 cut(s) | 455, 1056, 1308 |
| MaeIII | GTNAC | 6 cut(s) | 554, 1057, 1387, 1912, 2122, 2353 |
| MalI | GATC | 5 cut(s) | 1105, 1326, 1598, 1968, 2088 |
| MboI | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| MflI | RGATCY | 3 cut(s) | 1103, 1966, 2086 |
| MhlI | GDGCHC | 3 cut(s) | 1637, 1756, 1801 |
| MlyI | GAGTC | 4 cut(s) | 1133, 1352, 1496, 2350 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2112 |
| MseI | TTAA | 6 cut(s) | 321, 522, 597, 1275, 1577, 1608 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1860, 2043 |
| MspI | CCGG | 3 cut(s) | 116, 1957, 2043 |
| MspR9I | CCNGG | 4 cut(s) | 117, 417, 510, 1505 |
| MvaI | CCWGG | 3 cut(s) | 417, 510, 1505 |
| MvnI | CGCG | 1 cut(s) | 906 |
| NciI | CCSGG | 1 cut(s) | 117 |
| NdeI | CATATG | 1 cut(s) | 1519 |
| NdeII | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| NlaIV | GGNNCC | 9 cut(s) | 79, 421, 670, 819, 1144, 1475, 1635, 1817, 1968 |
| NmeAIII | GCCGAG | 1 cut(s) | 249 |
| NmuCI | GTSAC | 3 cut(s) | 554, 1387, 2353 |
| NspI | RCATGY | 3 cut(s) | 164, 1674, 2417 |
| NspV | TTCGAA | 1 cut(s) | 719 |
| PaeI | GCATGC | 1 cut(s) | 2417 |
| PaeR7I | CTCGAG | 1 cut(s) | 218 |
| PagI | TCATGA | 2 cut(s) | 724, 1459 |
| PceI | AGGCCT | 1 cut(s) | 941 |
| PciI | ACATGT | 2 cut(s) | 160, 1670 |
| PciSI | GCTCTTC | 1 cut(s) | 505 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2112 |
| PfeI | GAWTC | 8 cut(s) | 694, 721, 780, 788, 1063, 1448, 1987, 2010 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 142, 690 |
| PfoI | TCCNGGA | 1 cut(s) | 415 |
| PinAI | ACCGGT | 1 cut(s) | 2042 |
| PleI | GAGTC | 4 cut(s) | 1132, 1351, 1495, 2350 |
| PpsI | GAGTC | 4 cut(s) | 1132, 1351, 1495, 2350 |
| PpuMI | RGGWCCY | 1 cut(s) | 770 |
| PscI | ACATGT | 2 cut(s) | 160, 1670 |
| Psp1406I | AACGTT | 1 cut(s) | 1308 |
| Psp5II | RGGWCCY | 1 cut(s) | 770 |
| Psp6I | CCWGG | 3 cut(s) | 415, 508, 1503 |
| PspGI | CCWGG | 3 cut(s) | 415, 508, 1503 |
| PspN4I | GGNNCC | 9 cut(s) | 79, 421, 670, 819, 1144, 1475, 1635, 1817, 1968 |
| PspOMI | GGGCCC | 1 cut(s) | 1633 |
| PspPI | GGNCC | 8 cut(s) | 546, 770, 818, 1474, 1512, 1633, 1634, 1815 |
| PspPPI | RGGWCCY | 1 cut(s) | 770 |
| PstI | CTGCAG | 2 cut(s) | 172, 1261 |
| PstNI | CAGNNNCTG | 1 cut(s) | 509 |
| PsuI | RGATCY | 3 cut(s) | 1103, 1966, 2086 |
| RsaI | GTAC | 6 cut(s) | 159, 210, 463, 481, 538, 581 |
| RsaNI | GTAC | 6 cut(s) | 158, 209, 462, 480, 537, 580 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1860, 2043 |
| SapI | GCTCTTC | 1 cut(s) | 505 |
| SaqAI | TTAA | 6 cut(s) | 321, 522, 597, 1275, 1577, 1608 |
| Sau3AI | GATC | 5 cut(s) | 1103, 1324, 1596, 1966, 2086 |
| Sau96I | GGNCC | 8 cut(s) | 546, 770, 818, 1474, 1512, 1633, 1634, 1815 |
| ScaI | AGTACT | 1 cut(s) | 481 |
| SchI | GAGTC | 4 cut(s) | 1133, 1352, 1496, 2350 |
| ScrFI | CCNGG | 4 cut(s) | 117, 417, 510, 1505 |
| SduI | GDGCHC | 3 cut(s) | 1637, 1756, 1801 |
| SexAI | ACCWGGT | 1 cut(s) | 1503 |
| SfaNI | GCATC | 7 cut(s) | 558, 964, 1047, 1303, 1399, 1527, 2063 |
| SfcI | CTRYAG | 4 cut(s) | 168, 663, 1257, 1804 |
| Sfr274I | CTCGAG | 1 cut(s) | 218 |
| SfuI | TTCGAA | 1 cut(s) | 719 |
| SinI | GGWCC | 5 cut(s) | 546, 770, 818, 1474, 1815 |
| SlaI | CTCGAG | 1 cut(s) | 218 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1860, 2043 |
| SmlI | CTYRAG | 2 cut(s) | 218, 915 |
| SmoI | CTYRAG | 2 cut(s) | 218, 915 |
| SphI | GCATGC | 1 cut(s) | 2417 |
| SseBI | AGGCCT | 1 cut(s) | 941 |
| SsiI | CCGC | 7 cut(s) | 44, 59, 242, 946, 1146, 1515, 1594 |
| SspMI | CTAG | 7 cut(s) | 266, 1107, 1212, 1368, 1395, 1901, 2084 |
| StuI | AGGCCT | 1 cut(s) | 941 |
| StyD4I | CCNGG | 4 cut(s) | 115, 415, 508, 1503 |
| StyI | CCWWGG | 1 cut(s) | 1049 |
| TaaI | ACNGT | 5 cut(s) | 255, 550, 1441, 2103, 2326 |
| TaiI | ACGT | 3 cut(s) | 458, 1059, 1311 |
| TaqI | TCGA | 5 cut(s) | 153, 219, 398, 719, 1543 |
| TaqII | GACCGA | 1 cut(s) | 1491 |
| TatI | WGTACW | 4 cut(s) | 157, 461, 479, 579 |
| TauI | GCSGC | 2 cut(s) | 62, 1517 |
| TfiI | GAWTC | 8 cut(s) | 694, 721, 780, 788, 1063, 1448, 1987, 2010 |
| Tru1I | TTAA | 6 cut(s) | 321, 522, 597, 1275, 1577, 1608 |
| Tru9I | TTAA | 6 cut(s) | 321, 522, 597, 1275, 1577, 1608 |
| TscAI | CASTG | 5 cut(s) | 648, 790, 818, 1953, 2358 |
| TseFI | GTSAC | 3 cut(s) | 554, 1387, 2353 |
| TseI | GCWGC | 8 cut(s) | 167, 491, 851, 860, 920, 998, 1088, 2093 |
| Tsp45I | GTSAC | 3 cut(s) | 554, 1387, 2353 |
| TspGWI | ACGGA | 2 cut(s) | 1165, 2246 |
| TspRI | CASTG | 5 cut(s) | 648, 790, 818, 1953, 2358 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 142, 690 |
| VneI | GTGCAC | 1 cut(s) | 1752 |
| VpaK11BI | GGWCC | 5 cut(s) | 546, 770, 818, 1474, 1815 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 946 |
| XapI | RAATTY | 5 cut(s) | 86, 106, 715, 1221, 2314 |
| XbaI | TCTAGA | 1 cut(s) | 1106 |
| XceI | RCATGY | 3 cut(s) | 164, 1674, 2417 |
| XhoI | CTCGAG | 1 cut(s) | 218 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2112 |
| XspI | CTAG | 7 cut(s) | 266, 1107, 1212, 1368, 1395, 1901, 2084 |
| ZrmI | AGTACT | 1 cut(s) | 481 |