Rh7BG263900

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001305
Physical Location & Seq
Forward (+)
24103598 .. 24104881
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG263900.1

Sequence Viewer

Length: 1284 bp
ATGAGAAAAAAGTATAAGAAACTAGCTCTTGTTCTTCACCCTGATAAGAACGGTTCTCCAGGAGCTGATGGAGCATTCATGCTGATTGCAGAGGCGTGGAGTTTGTTGTCTGATAAAGCCAAGAGAGCAGCATATGACCAGAAGATCAGGAATGAAAGATTTCACAGAAATGTTTCGAGTACGGTGGCCCAGGATTTTCCCAATTTCACACAAAGCACAAACTTCAGTGCTGCAAACGGTCAGACGACAACCACACAGGCGGCTAGTTTTTCGACTCCGGCTGCTTCATCCCAAACACCCAATACCTTTAGGACCATGTGTCCTACTTGCTTGTTGACATATGAGCATCTCCTCATTTACCTTCATCATTATCTTCGTTGCCATCGTTGCCAAAGTACACTTTTACCAATAGACATTGCTCCACCTATAATTGCGTCAGAGCAGTTCACCCCATCAAATTTAGCAGAGCAGTGGCAGAATTCAAATCATGAAACAACAGCGAATAGAAGCACATTCAATACAGGAAGGAGTAGTGCAGCCTCTGCAATTAACCAAAGCTTCTACCATACGTTCTCGAGGACTGCTAGTGGTTTTACAGCTGCCCATGCCCCAAGCGTGGATCAGATTATGGCACAAACGGCAACTAGAATGCCTCATGCCTTCAATCAGCCCAGTGGTGCTTCAACTACAGGATATGCTAATAATGCTGCAAAGAGAAGATGCATGGATGATGTTGGAATGAGCAGATATGGAAAGGAAGCTAAAAATCATATGGATCGGGGAGCTGTGGAGGCTACTTTTGGACCATTCAGGGCTTATGGAGCCTCGAATACCATGTTCACAAGTAATGATAGCTCCCTACATGCAACACAGAATATACAAAAAGAGAAGACTAAGAAGGAAATGAAGGGACAGGGATCATCTAGTGTAGCCAAGACTGCAGTAAAGGCGGAGAATGGAAATGTGAGAGGAAAGTCTTCGGGGCACTCTGATGTACAGTGTGATCACAGCATGTCTGGCATTGGCAAGCAGGTGGATACAAAGAATACCATTCTCACAAGTAATGATATCTCCCTACATGCAACACAGAATATACTAAAAGAGAAGACTAGGAAGAAAATGAGTGAACGAGGATCATCTAGTGTAGCCAAAACTGCAGTAAACAAGGCAGAGAATGCAAATGAGAGAGGAAAATCTTCAAGAAACTCTGATATGCAGTGTGATCGCAACATGTCCGGCATTGGCAAGCTGGTGGATACAAAGAACATTGGTACATGCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

427

Amino Acids

46.55

Weight (kDa)

9.66

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 2 - 46 6.2e-14 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1021
Acc36I ACCTGC 1 cut(s) 1021
AciI CCGC 2 cut(s) 260, 950
AclWI GGATC 4 cut(s) 627, 783, 925, 1141
AcsI RAATTY 2 cut(s) 457, 478
AcuI CTGAAG 1 cut(s) 208
AfaI GTAC 4 cut(s) 181, 397, 996, 1273
AfiI CCNNNNNNNGG 1 cut(s) 616
AflIII ACRYGT 1 cut(s) 1230
AgsI TTSAA 5 cut(s) 483, 517, 664, 684, 1200
AhdI GACNNNNNGTC 1 cut(s) 318
AjnI CCWGG 2 cut(s) 58, 189
AluBI AGCT 8 cut(s) 26, 65, 558, 599, 761, 785, 855, 1249
AluI AGCT 8 cut(s) 26, 65, 558, 599, 761, 785, 855, 1249
AlwI GGATC 4 cut(s) 627, 783, 925, 1141
AlwNI CAGNNNCTG 2 cut(s) 65, 542
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 1 cut(s) 186
ApeKI GCWGC 6 cut(s) 128, 230, 281, 536, 599, 707
ApoI RAATTY 2 cut(s) 457, 478
Asp700I GAANNNNTTC 4 cut(s) 159, 172, 976, 1195
AspS9I GGNCC 3 cut(s) 187, 312, 803
AsuHPI GGTGA 2 cut(s) 29, 439
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 2 cut(s) 312, 803
BaeGI GKGCMC 1 cut(s) 987
BbsI GAAGAC 3 cut(s) 896, 969, 1112
BbvI GCAGC 6 cut(s) 140, 217, 268, 548, 586, 694
BccI CCATC 3 cut(s) 62, 390, 460
BceAI ACGGC 1 cut(s) 654
BcgI CGANNNNNNTGC 2 cut(s) 1205, 1239
BciT130I CCWGG 2 cut(s) 60, 191
BciVI GTATCC 2 cut(s) 1030, 1249
BclI TGATCA 1 cut(s) 1003
BfaI CTAG 7 cut(s) 23, 264, 585, 645, 924, 1110, 1140
BfmI CTRYAG 3 cut(s) 687, 939, 1155
BfuAI ACCTGC 1 cut(s) 1021
BfuI GTATCC 2 cut(s) 1030, 1249
BisI GCNGC 7 cut(s) 129, 231, 261, 282, 537, 600, 708
BlsI GCNGC 7 cut(s) 130, 232, 262, 283, 538, 601, 709
Bme1390I CCNGG 2 cut(s) 60, 191
Bme18I GGWCC 2 cut(s) 312, 803
BmeRI GACNNNNNGTC 1 cut(s) 318
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 3 cut(s) 187, 312, 803
BmiI GGNNCC 1 cut(s) 823
BmrFI CCNGG 2 cut(s) 60, 191
BmrI ACTGGG 1 cut(s) 666
BmsI GCATC 2 cut(s) 355, 710
BmuI ACTGGG 1 cut(s) 666
BpiI GAAGAC 3 cut(s) 896, 969, 1112
BpmI CTGGAG 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse1I ACTGG 1 cut(s) 672
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 2 cut(s) 60, 191
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 287, 733
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMI GCAATG 1 cut(s) 414
BseNI ACTGG 1 cut(s) 672
BseRI GAGGAG 1 cut(s) 341
BseSI GKGCMC 1 cut(s) 987
BseXI GCAGC 6 cut(s) 140, 217, 268, 548, 586, 694
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 2 cut(s) 278, 1236
BslFI GGGAC 1 cut(s) 924
BslI CCNNNNNNNGG 1 cut(s) 616
BsmFI GGGAC 1 cut(s) 924
BsmI GAATGC 3 cut(s) 74, 654, 1180
BsnI GGCC 1 cut(s) 188
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 1 cut(s) 987
Bsp1407I TGTACA 1 cut(s) 994
Bsp143I GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
BspACI CCGC 2 cut(s) 260, 950
BspANI GGCC 1 cut(s) 188
BspHI TCATGA 1 cut(s) 487
BspLI GGNNCC 1 cut(s) 823
BspMAI CTGCAG 2 cut(s) 943, 1159
BspMI ACCTGC 1 cut(s) 1021
BspPI GGATC 4 cut(s) 627, 783, 925, 1141
BsrDI GCAATG 1 cut(s) 414
BsrGI TGTACA 1 cut(s) 994
BsrI ACTGG 1 cut(s) 672
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
Bst2UI CCWGG 2 cut(s) 60, 191
Bst4CI ACNGT 4 cut(s) 53, 184, 239, 999
BstAPI GCANNNNNTGC 2 cut(s) 542, 1175
BstAUI TGTACA 1 cut(s) 994
BstC8I GCNNGC 2 cut(s) 1028, 1247
BstDEI CTNAG 1 cut(s) 894
BstF5I GGATG 2 cut(s) 287, 733
BstKTI GATC 7 cut(s) 147, 622, 778, 920, 1006, 1136, 1225
BstMBI GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
BstNI CCWGG 2 cut(s) 60, 191
BstNSI RCATGY 6 cut(s) 866, 1015, 1082, 1234, 1278, 1282
BstSCI CCNGG 2 cut(s) 58, 189
BstSFI CTRYAG 3 cut(s) 687, 939, 1155
BstSLI GKGCMC 1 cut(s) 987
BstV1I GCAGC 6 cut(s) 140, 217, 268, 548, 586, 694
BstV2I GAAGAC 3 cut(s) 896, 969, 1112
BsuI GTATCC 2 cut(s) 1030, 1249
BsuRI GGCC 1 cut(s) 188
BtsCI GGATG 2 cut(s) 287, 733
BtsI GCAGTG 2 cut(s) 476, 1223
BtsIMutI CAGTG 5 cut(s) 232, 476, 679, 1004, 1223
BveI ACCTGC 1 cut(s) 1021
Cac8I GCNNGC 2 cut(s) 1028, 1247
CaiI CAGNNNCTG 2 cut(s) 65, 542
CciI TCATGA 1 cut(s) 487
Cfr13I GGNCC 3 cut(s) 187, 312, 803
CseI GACGC 1 cut(s) 423
Csp6I GTAC 4 cut(s) 180, 396, 995, 1272
CviQI GTAC 4 cut(s) 180, 396, 995, 1272
DdeI CTNAG 1 cut(s) 894
DpnI GATC 7 cut(s) 146, 621, 777, 919, 1005, 1135, 1224
DpnII GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
DriI GACNNNNNGTC 1 cut(s) 318
Eam1105I GACNNNNNGTC 1 cut(s) 318
EciI GGCGGA 1 cut(s) 965
Eco32I GATATC 1 cut(s) 1069
Eco47I GGWCC 2 cut(s) 312, 803
Eco57I CTGAAG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 574
EcoRI GAATTC 1 cut(s) 478
EcoRII CCWGG 2 cut(s) 58, 189
EcoRV GATATC 1 cut(s) 1069
EcoT22I ATGCAT 2 cut(s) 725, 1280
FaqI GGGAC 1 cut(s) 924
FauNDI CATATG 3 cut(s) 133, 340, 771
FbaI TGATCA 1 cut(s) 1003
Fnu4HI GCNGC 7 cut(s) 129, 231, 261, 282, 537, 600, 708
FokI GGATG 2 cut(s) 274, 740
Fsp4HI GCNGC 7 cut(s) 129, 231, 261, 282, 537, 600, 708
FspBI CTAG 7 cut(s) 23, 264, 585, 645, 924, 1110, 1140
GluI GCNGC 7 cut(s) 129, 231, 261, 282, 537, 600, 708
GsuI CTGGAG 1 cut(s) 42
HaeIII GGCC 1 cut(s) 188
HapII CCGG 2 cut(s) 278, 1236
HgaI GACGC 1 cut(s) 423
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 556
HinfI GANTC 1 cut(s) 274
HpaII CCGG 2 cut(s) 278, 1236
HphI GGTGA 2 cut(s) 29, 439
Hpy166II GTNNAC 6 cut(s) 336, 398, 447, 840, 1127, 1162
Hpy188I TCNGA 6 cut(s) 112, 243, 439, 624, 991, 1210
Hpy188III TCNNGA 4 cut(s) 148, 488, 574, 1200
Hpy8I GTNNAC 6 cut(s) 336, 398, 447, 840, 1127, 1162
HpyAV CCTTC 5 cut(s) 371, 519, 670, 892, 901
HpyCH4III ACNGT 4 cut(s) 53, 184, 239, 999
HpyCH4IV ACGT 1 cut(s) 569
HpyF3I CTNAG 1 cut(s) 894
HpySE526I ACGT 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 1003
Kzo9I GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
LmnI GCTCC 6 cut(s) 62, 71, 424, 782, 821, 860
Lsp1109I GCAGC 6 cut(s) 140, 217, 268, 548, 586, 694
LweI GCATC 2 cut(s) 355, 710
MaeI CTAG 7 cut(s) 23, 264, 585, 645, 924, 1110, 1140
MaeII ACGT 1 cut(s) 569
MalI GATC 7 cut(s) 146, 621, 777, 919, 1005, 1135, 1224
MboI GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
MboII GAAGA 9 cut(s) 26, 154, 365, 729, 901, 969, 1117, 1126, 1188
MhlI GDGCHC 1 cut(s) 987
MluCI AATT 5 cut(s) 202, 429, 457, 478, 546
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 1 cut(s) 715
Mph1103I ATGCAT 2 cut(s) 725, 1280
MroXI GAANNNNTTC 4 cut(s) 159, 172, 976, 1195
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 3 cut(s) 168, 990, 1279
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 2 cut(s) 278, 1236
MspR9I CCNGG 2 cut(s) 60, 191
Mva1269I GAATGC 3 cut(s) 74, 654, 1180
MvaI CCWGG 2 cut(s) 60, 191
NdeI CATATG 3 cut(s) 133, 340, 771
NdeII GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
NlaIV GGNNCC 1 cut(s) 823
NsiI ATGCAT 2 cut(s) 725, 1280
NspI RCATGY 6 cut(s) 866, 1015, 1082, 1234, 1278, 1282
PaeR7I CTCGAG 1 cut(s) 574
PagI TCATGA 1 cut(s) 487
PaqCI CACCTGC 1 cut(s) 1021
PciI ACATGT 1 cut(s) 1230
PcsI WCGNNNNNNNCGW 1 cut(s) 382
PctI GAATGC 3 cut(s) 74, 654, 1180
PdmI GAANNNNTTC 4 cut(s) 159, 172, 976, 1195
PfoI TCCNGGA 1 cut(s) 58
PkrI GCNGC 7 cut(s) 130, 232, 262, 283, 538, 601, 709
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PscI ACATGT 1 cut(s) 1230
Psp6I CCWGG 2 cut(s) 58, 189
PspGI CCWGG 2 cut(s) 58, 189
PspN4I GGNNCC 1 cut(s) 823
PspPI GGNCC 3 cut(s) 187, 312, 803
PstI CTGCAG 2 cut(s) 943, 1159
PstNI CAGNNNCTG 2 cut(s) 65, 542
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 4 cut(s) 181, 397, 996, 1273
RsaNI GTAC 4 cut(s) 180, 396, 995, 1272
RseI CAYNNNNRTG 3 cut(s) 168, 990, 1279
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 7 cut(s) 129, 231, 261, 282, 537, 600, 708
Sau3AI GATC 7 cut(s) 144, 619, 775, 917, 1003, 1133, 1222
Sau96I GGNCC 3 cut(s) 187, 312, 803
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 2 cut(s) 60, 191
SduI GDGCHC 1 cut(s) 987
SfaNI GCATC 2 cut(s) 355, 710
SfcI CTRYAG 3 cut(s) 687, 939, 1155
Sfr274I CTCGAG 1 cut(s) 574
SinI GGWCC 2 cut(s) 312, 803
SlaI CTCGAG 1 cut(s) 574
SmiMI CAYNNNNRTG 3 cut(s) 168, 990, 1279
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
Sse9I AATT 5 cut(s) 202, 429, 457, 478, 546
SsiI CCGC 2 cut(s) 260, 950
SspMI CTAG 7 cut(s) 23, 264, 585, 645, 924, 1110, 1140
StyD4I CCNGG 2 cut(s) 58, 189
TaaI ACNGT 4 cut(s) 53, 184, 239, 999
TaiI ACGT 1 cut(s) 572
TaqI TCGA 4 cut(s) 176, 272, 575, 827
TasI AATT 5 cut(s) 202, 429, 457, 478, 546
TatI WGTACW 2 cut(s) 395, 994
TauI GCSGC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 5 cut(s) 232, 476, 679, 1004, 1223
TseI GCWGC 6 cut(s) 128, 230, 281, 536, 599, 707
TspDTI ATGAA 6 cut(s) 67, 168, 276, 353, 504, 920
TspRI CASTG 5 cut(s) 232, 476, 679, 1004, 1223
VpaK11BI GGWCC 2 cut(s) 312, 803
XapI RAATTY 2 cut(s) 457, 478
XceI RCATGY 6 cut(s) 866, 1015, 1082, 1234, 1278, 1282
XhoI CTCGAG 1 cut(s) 574
XmnI GAANNNNTTC 4 cut(s) 159, 172, 976, 1195
XspI CTAG 7 cut(s) 23, 264, 585, 645, 924, 1110, 1140
Zsp2I ATGCAT 2 cut(s) 725, 1280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.