FvH4_4g23600

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001305
Physical Location & Seq
Forward (+)
26045168 .. 26048217
3050 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23600.t3

Sequence Viewer

Length: 1314 bp
ATGGAGTGCAATAAAGATGATGGCGTCAGGGCAAAAGAGATTGCGGAGAGGAAGTTCAGTGAAAGGGATATAATGGGCGCGAAGAAATTTGCGTTGAAGGCTCAAGCCTTGTATCCGGGGCTGGATGGTATCCGGCAACTTCTGGCGACACTTGATGTATATATGTGTGCCACTGCAGAGAATAAGATAGGTGGGGAACCTGATTGGTATGGCATACTTGGTGTGGATCCTGAGGCAGATGATGAGACGGTGAAGAAACGGTACAGAACACTAGCTCTTAACCTTCACCCTGATAAAAATAAGTCTGCAGGGGCTGGTGGAGCATTCAACTTAGTTAAGGACGCATGTGATTTGTTGTCTGATAAAAATAAGAGAGCAGCATATGACAGGAGAAGGGATGTACATGTGCGCCAAAATGTGGTACACCAGAATATTTCAACTACAAATCAGGCTTCAAAGGAGGCCAATGGTTCTGTACACCAGAAAGTTTCAACTGCGCAAAGTTCACAGGGGGCTAATGGTTCCGCTCACCAGAAATTTTCAACTGGAGCTCAAGGTTCACATGGGGCCAATGGTTCTGCTCACCAGAGAGTTCCAACTGGAGTTCAAGGTTCGCATGGGGCTAATGGTTCTGCTCACCAGAAATTTTCAACTGGAGCTCAAGATTCACATGGGGCCAATGGTACAACAACAAAGGGTAGAGAATCCAATGCAAAGGCTCAGACGCGCACCAAACGTCCAGCTCCCTCTTCTGCTCCTGCTTCTGTCAAACCAATACCCAAAAGCTTTTGGTGCGAGTGTCCAAAATGCTGGATGCGATTCGAGTACTTAGGAAAGTATCTCAGGTGGAATGGTTTTTGCCCGAATTGTAATAAACTATTTCAGGCAGTAGAAATTGTTCCACCAGCCATGTGTGGTTCAAAGTCTACCACCCCAGTCCATACAGGAAGGAGTACTTCAGCCTCTGAAAATGTTGGTGCGACAGGTTTTATCAGTCATGTGTCATTCACACAAAAGAGTGTTATCTGGGCTCCATTTTCAAGAGCTGCTAGTGCTTCTCAAGCTGCCAAAGCTGCAAAGGTAGACTTCCATGCATCTGGCAGAGCTGCCGGCCCTTCTAAATCTGCCAAAGCTTCAGGTGGGGATAAGCTAGCATCTAAGAGAGGGAGGAAATGCCCTTTTGATGAGGACACACTGGCAAGAGCAAAAGAGAGTTTCACTTGGGATCTAAATGATGACGAAAGAAAGAAGAGGTTCGATGCCAGAAGAAAGGACTTTGATGCCAATGGAAGGAGGGATGATGGTATTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

438

Amino Acids

47.16

Weight (kDa)

9.63

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 68 - 129 1.5e-20 DnaJ domain
Zn_ribbon_20 PF23551 227 - 261 5.2e-06 Zinc beta-ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 498
AccB7I CCANNNNNTGG 1 cut(s) 418
AccBSI CCGCTC 1 cut(s) 527
AccI GTMKAC 2 cut(s) 926, 1083
AccII CGCG 2 cut(s) 80, 727
AciI CCGC 2 cut(s) 44, 525
AclWI GGATC 3 cut(s) 221, 234, 1233
AcsI RAATTY 3 cut(s) 86, 536, 644
AcuI CTGAAG 2 cut(s) 942, 1119
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 7 cut(s) 263, 402, 423, 477, 685, 827, 955
AfiI CCNNNNNNNGG 2 cut(s) 418, 1290
AflIII ACRYGT 1 cut(s) 403
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
Alw21I GWGCWC 2 cut(s) 553, 661
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 3 cut(s) 221, 234, 1233
AlwNI CAGNNNCTG 2 cut(s) 314, 965
AoxI GGCC 4 cut(s) 462, 567, 675, 1111
ApeKI GCWGC 5 cut(s) 377, 1046, 1064, 1073, 1106
ApoI RAATTY 3 cut(s) 86, 536, 644
Asp700I GAANNNNTTC 2 cut(s) 897, 1253
AspLEI GCGC 4 cut(s) 80, 411, 499, 729
AspS9I GGNCC 3 cut(s) 567, 675, 1112
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 5 cut(s) 262, 278, 521, 575, 629
AsuNHI GCTAGC 1 cut(s) 1150
AxyI CCTNAGG 1 cut(s) 231
BamHI GGATCC 1 cut(s) 226
BanII GRGCYC 3 cut(s) 553, 661, 1033
BarI GAAGNNNNNNTAC 2 cut(s) 245, 277
Bbv12I GWGCWC 2 cut(s) 553, 661
BbvI GCAGC 5 cut(s) 389, 1033, 1051, 1060, 1093
BccI CCATC 3 cut(s) 14, 119, 1295
BciVI GTATCC 2 cut(s) 123, 140
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 3 cut(s) 272, 1050, 1151
BfmI CTRYAG 3 cut(s) 174, 306, 1310
BfuI GTATCC 2 cut(s) 123, 140
BisI GCNGC 5 cut(s) 378, 1047, 1065, 1074, 1107
BlsI GCNGC 5 cut(s) 379, 1048, 1066, 1075, 1108
BmcAI AGTACT 2 cut(s) 827, 955
Bme1390I CCNGG 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 567, 675, 1112
BmiI GGNNCC 6 cut(s) 198, 228, 523, 568, 676, 1032
BmrFI CCNGG 1 cut(s) 117
BmrI ACTGGG 1 cut(s) 929
BmsI GCATC 5 cut(s) 804, 1103, 1163, 1249, 1270
BmtI GCTAGC 1 cut(s) 1154
BmuI ACTGGG 1 cut(s) 929
BpmI CTGGAG 3 cut(s) 567, 621, 675
BpuEI CTTGAG 4 cut(s) 87, 537, 645, 1044
BpuMI CCSGG 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 1290
Bse118I RCCGGY 1 cut(s) 1109
Bse1I ACTGG 5 cut(s) 550, 604, 658, 935, 1200
Bse21I CCTNAGG 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 4 cut(s) 130, 403, 819, 1303
BseLI CCNNNNNNNGG 2 cut(s) 418, 1290
BseMII CTCAG 3 cut(s) 222, 734, 856
BseNI ACTGG 5 cut(s) 550, 604, 658, 935, 1200
BseXI GCAGC 5 cut(s) 389, 1033, 1051, 1060, 1093
Bsh1236I CGCG 2 cut(s) 80, 727
BshFI GGCC 4 cut(s) 464, 569, 677, 1113
BsiHKAI GWGCWC 2 cut(s) 553, 661
BsiSI CCGG 3 cut(s) 116, 133, 1110
BslI CCNNNNNNNGG 2 cut(s) 418, 1290
BsmAI GTCTC 1 cut(s) 239
BsmBI CGTCTC 1 cut(s) 239
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 4 cut(s) 464, 569, 677, 1113
Bsp1286I GDGCHC 3 cut(s) 553, 661, 1033
Bsp1407I TGTACA 2 cut(s) 400, 475
Bsp143I GATC 2 cut(s) 226, 1225
BspACI CCGC 2 cut(s) 44, 525
BspANI GGCC 4 cut(s) 464, 569, 677, 1113
BspCNI CTCAG 3 cut(s) 223, 733, 855
BspFNI CGCG 2 cut(s) 80, 727
BspLI GGNNCC 6 cut(s) 198, 228, 523, 568, 676, 1032
BspMAI CTGCAG 2 cut(s) 178, 310
BspOI GCTAGC 1 cut(s) 1154
BspPI GGATC 3 cut(s) 221, 234, 1233
BsrBI CCGCTC 1 cut(s) 527
BsrFI RCCGGY 1 cut(s) 1109
BsrGI TGTACA 2 cut(s) 400, 475
BsrI ACTGG 5 cut(s) 550, 604, 658, 935, 1200
BssAI RCCGGY 1 cut(s) 1109
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 2 cut(s) 226, 1225
BssNI GRCGYC 1 cut(s) 24
Bst4CI ACNGT 2 cut(s) 250, 261
Bst6I CTCTTC 2 cut(s) 754, 1244
BstACI GRCGYC 1 cut(s) 24
BstAUI TGTACA 2 cut(s) 400, 475
BstC8I GCNNGC 2 cut(s) 1111, 1152
BstDEI CTNAG 6 cut(s) 231, 331, 720, 829, 842, 1158
BstF5I GGATG 4 cut(s) 130, 403, 819, 1303
BstFNI CGCG 2 cut(s) 80, 727
BstHHI GCGC 4 cut(s) 80, 411, 499, 729
BstKTI GATC 2 cut(s) 229, 1228
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 2 cut(s) 226, 1225
BstMWI GCNNNNNNNGC 7 cut(s) 98, 320, 792, 1052, 1061, 1070, 1073
BstNSI RCATGY 2 cut(s) 348, 407
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 3 cut(s) 174, 306, 1310
BstUI CGCG 2 cut(s) 80, 727
BstV1I GCAGC 5 cut(s) 389, 1033, 1051, 1060, 1093
BstX2I RGATCY 2 cut(s) 226, 1225
BstXI CCANNNNNNTGG 2 cut(s) 810, 1097
BstYI RGATCY 2 cut(s) 226, 1225
Bsu36I CCTNAGG 1 cut(s) 231
BsuI GTATCC 2 cut(s) 123, 140
BsuRI GGCC 4 cut(s) 464, 569, 677, 1113
BtsCI GGATG 4 cut(s) 130, 403, 819, 1303
BtsI GCAGTG 1 cut(s) 171
BtsIMutI CAGTG 3 cut(s) 64, 171, 1193
Cac8I GCNNGC 2 cut(s) 1111, 1152
CaiI CAGNNNCTG 2 cut(s) 314, 965
CfoI GCGC 4 cut(s) 80, 411, 499, 729
Cfr10I RCCGGY 1 cut(s) 1109
Cfr13I GGNCC 3 cut(s) 567, 675, 1112
CseI GACGC 3 cut(s) 13, 350, 733
Csp6I GTAC 7 cut(s) 262, 401, 422, 476, 684, 826, 954
CviAII CATG 8 cut(s) 345, 404, 563, 617, 671, 910, 998, 1091
CviQI GTAC 7 cut(s) 262, 401, 422, 476, 684, 826, 954
DdeI CTNAG 6 cut(s) 231, 331, 720, 829, 842, 1158
DpnI GATC 2 cut(s) 228, 1227
DpnII GATC 2 cut(s) 226, 1225
Eam1104I CTCTTC 2 cut(s) 754, 1244
EarI CTCTTC 2 cut(s) 754, 1244
Ecl136II GAGCTC 2 cut(s) 551, 659
Eco24I GRGCYC 3 cut(s) 553, 661, 1033
Eco53kI GAGCTC 2 cut(s) 551, 659
Eco57I CTGAAG 2 cut(s) 942, 1119
Eco81I CCTNAGG 1 cut(s) 231
EcoICRI GAGCTC 2 cut(s) 551, 659
EcoT22I ATGCAT 1 cut(s) 1096
EcoT38I GRGCYC 3 cut(s) 553, 661, 1033
Esp3I CGTCTC 1 cut(s) 239
FaeI CATG 8 cut(s) 348, 407, 566, 620, 674, 913, 1001, 1094
FalI AAGNNNNNCTT 4 cut(s) 940, 972, 1070, 1102
FatI CATG 8 cut(s) 344, 403, 562, 616, 670, 909, 997, 1090
FauNDI CATATG 1 cut(s) 382
FblI GTMKAC 2 cut(s) 926, 1083
Fnu4HI GCNGC 5 cut(s) 378, 1047, 1065, 1074, 1107
FokI GGATG 4 cut(s) 137, 410, 826, 1310
FriOI GRGCYC 3 cut(s) 553, 661, 1033
Fsp4HI GCNGC 5 cut(s) 378, 1047, 1065, 1074, 1107
FspBI CTAG 3 cut(s) 272, 1050, 1151
FspI TGCGCA 1 cut(s) 498
GlaI GCGC 4 cut(s) 79, 410, 498, 728
GluI GCNGC 5 cut(s) 378, 1047, 1065, 1074, 1107
GsuI CTGGAG 3 cut(s) 567, 621, 675
HaeIII GGCC 4 cut(s) 464, 569, 677, 1113
HapII CCGG 3 cut(s) 116, 133, 1110
HgaI GACGC 3 cut(s) 13, 350, 733
HhaI GCGC 4 cut(s) 80, 411, 499, 729
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 8 cut(s) 348, 407, 566, 620, 674, 913, 1001, 1094
Hin6I GCGC 4 cut(s) 78, 409, 497, 727
HinP1I GCGC 4 cut(s) 78, 409, 497, 727
HindIII AAGCTT 2 cut(s) 784, 1131
HinfI GANTC 3 cut(s) 665, 704, 819
HpaII CCGG 3 cut(s) 116, 133, 1110
HphI GGTGA 5 cut(s) 262, 278, 521, 575, 629
Hpy166II GTNNAC 6 cut(s) 424, 478, 506, 560, 927, 1084
Hpy188I TCNGA 3 cut(s) 361, 723, 967
Hpy188III TCNNGA 3 cut(s) 230, 662, 1041
Hpy8I GTNNAC 6 cut(s) 424, 478, 506, 560, 927, 1084
HpyAV CCTTC 6 cut(s) 91, 293, 387, 942, 1125, 1284
HpyCH4III ACNGT 2 cut(s) 250, 261
HpyCH4IV ACGT 1 cut(s) 736
HpyCH4V TGCA 6 cut(s) 9, 176, 308, 713, 1076, 1094
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 320, 792, 1052, 1061, 1070, 1073
HpyF3I CTNAG 6 cut(s) 231, 331, 720, 829, 842, 1158
HpySE526I ACGT 1 cut(s) 736
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 8 cut(s) 348, 407, 566, 620, 674, 913, 1001, 1094
HspAI GCGC 4 cut(s) 78, 409, 497, 727
KroI GCCGGC 1 cut(s) 1109
KroNI GCCGGC 1 cut(s) 1111
Kzo9I GATC 2 cut(s) 226, 1225
LmnI GCTCC 6 cut(s) 320, 548, 656, 748, 760, 1036
Lsp1109I GCAGC 5 cut(s) 389, 1033, 1051, 1060, 1093
LweI GCATC 5 cut(s) 804, 1103, 1163, 1249, 1270
MaeI CTAG 3 cut(s) 272, 1050, 1151
MaeII ACGT 1 cut(s) 736
MalI GATC 2 cut(s) 228, 1227
MbiI CCGCTC 1 cut(s) 527
MboI GATC 2 cut(s) 226, 1225
MboII GAAGA 5 cut(s) 94, 265, 741, 1261, 1278
MflI RGATCY 2 cut(s) 226, 1225
MhlI GDGCHC 3 cut(s) 553, 661, 1033
MluCI AATT 5 cut(s) 86, 536, 644, 865, 894
MmeI TCCRAC 1 cut(s) 620
Mph1103I ATGCAT 1 cut(s) 1096
MroNI GCCGGC 1 cut(s) 1109
MroXI GAANNNNTTC 2 cut(s) 897, 1253
MseI TTAA 2 cut(s) 279, 336
MspI CCGG 3 cut(s) 116, 133, 1110
MspR9I CCNGG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 323
MvnI CGCG 2 cut(s) 80, 727
MwoI GCNNNNNNNGC 7 cut(s) 98, 320, 792, 1052, 1061, 1070, 1073
NaeI GCCGGC 1 cut(s) 1111
NciI CCSGG 1 cut(s) 117
NdeI CATATG 1 cut(s) 382
NdeII GATC 2 cut(s) 226, 1225
NgoMIV GCCGGC 1 cut(s) 1109
NheI GCTAGC 1 cut(s) 1150
NlaIII CATG 8 cut(s) 348, 407, 566, 620, 674, 913, 1001, 1094
NlaIV GGNNCC 6 cut(s) 198, 228, 523, 568, 676, 1032
NsbI TGCGCA 1 cut(s) 498
NsiI ATGCAT 1 cut(s) 1096
NspI RCATGY 2 cut(s) 348, 407
PciI ACATGT 1 cut(s) 403
PctI GAATGC 1 cut(s) 323
PdiI GCCGGC 1 cut(s) 1111
PdmI GAANNNNTTC 2 cut(s) 897, 1253
PfeI GAWTC 3 cut(s) 665, 704, 819
PflMI CCANNNNNTGG 1 cut(s) 418
PkrI GCNGC 5 cut(s) 379, 1048, 1066, 1075, 1108
PscI ACATGT 1 cut(s) 403
Psp124BI GAGCTC 2 cut(s) 553, 661
PspN4I GGNNCC 6 cut(s) 198, 228, 523, 568, 676, 1032
PspPI GGNCC 3 cut(s) 567, 675, 1112
PsrI GAACNNNNNNTAC 2 cut(s) 882, 914
PstI CTGCAG 2 cut(s) 178, 310
PstNI CAGNNNCTG 2 cut(s) 314, 965
PsuI RGATCY 2 cut(s) 226, 1225
RsaI GTAC 7 cut(s) 263, 402, 423, 477, 685, 827, 955
RsaNI GTAC 7 cut(s) 262, 401, 422, 476, 684, 826, 954
SacI GAGCTC 2 cut(s) 553, 661
SaqAI TTAA 2 cut(s) 279, 336
SatI GCNGC 5 cut(s) 378, 1047, 1065, 1074, 1107
Sau3AI GATC 2 cut(s) 226, 1225
Sau96I GGNCC 3 cut(s) 567, 675, 1112
ScaI AGTACT 2 cut(s) 827, 955
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 3 cut(s) 553, 661, 1033
SfaNI GCATC 5 cut(s) 804, 1103, 1163, 1249, 1270
SfcI CTRYAG 3 cut(s) 174, 306, 1310
SmlI CTYRAG 4 cut(s) 102, 552, 660, 1059
SmoI CTYRAG 4 cut(s) 102, 552, 660, 1059
Sse9I AATT 5 cut(s) 86, 536, 644, 865, 894
SsiI CCGC 2 cut(s) 44, 525
SspI AATATT 1 cut(s) 433
SspMI CTAG 3 cut(s) 272, 1050, 1151
SstI GAGCTC 2 cut(s) 553, 661
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 250, 261
TaiI ACGT 1 cut(s) 739
TaqI TCGA 2 cut(s) 822, 1257
TasI AATT 5 cut(s) 86, 536, 644, 865, 894
TatI WGTACW 4 cut(s) 400, 475, 825, 953
TfiI GAWTC 3 cut(s) 665, 704, 819
Tru1I TTAA 2 cut(s) 279, 336
Tru9I TTAA 2 cut(s) 279, 336
TscAI CASTG 3 cut(s) 64, 178, 1200
TseI GCWGC 5 cut(s) 377, 1046, 1064, 1073, 1106
TspRI CASTG 3 cut(s) 64, 178, 1200
Van91I CCANNNNNTGG 1 cut(s) 418
XapI RAATTY 3 cut(s) 86, 536, 644
XceI RCATGY 2 cut(s) 348, 407
XmiI GTMKAC 2 cut(s) 926, 1083
XmnI GAANNNNTTC 2 cut(s) 897, 1253
XspI CTAG 3 cut(s) 272, 1050, 1151
ZrmI AGTACT 2 cut(s) 827, 955
Zsp2I ATGCAT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.