Rw7G023180

Phosphoribulokinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014052
Physical Location & Seq
Forward (+)
27789542 .. 27793031
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023180.1

Sequence Viewer

Length: 1224 bp
ATGGCCATTTGTGCAGTCCACACAAGTACTCAGTTCAACCCAACATGCTCATCAATCTCCACCCCCTCAAAATCCCACTTGGGATTCTGCCAAAGACAACAAGTAGTGTTCTACACCAAGAGGAGTAGCAAGAGATCCACCCCATGCGTAGGTCTGATAGCTTGCGCCGCGGGTGGCCAGGAGACTGTGGTGATTGGCCTCGCCGCCGACTCCGGTTGCGGGAAGAGCACATTCATGAGGAGGTTGACAAGTGTGTTTGGAGGAGCAGCAGAGCCACCAAAGGGAGGCAACCCAGACTCCAACACTTTGATAAGTGACACCACCACTGTGATATGCTTGGATGATTACCACTCACTTGATAGGACTGGAAGGAAGGAGAAGGGAGTCACTGCTCTTGACCCAAGAGCCAACGACTTTGACCTCATGTATGAGCAAGTCAAGGCTATCAAGGATGGCAAGGCTGTTGATAAACCCATATACAACCATGTTAGTGGACTCTTGGACCCTCCAGAGCTTATCAAGCCCCCAAAGATCCTTGTCATTGAAGGCTTGCACCCAATGTACGACCAGCGAGTCAGGGACCTCTTGGACTTCAGTATCTACTTGGACATCAGCAATGAGGTTAAGTTTGCATGGAAAATTCAGAGGGACATGGCCGAGCGTGGACACAGTCTCGAGAGCATCAAAGCCAGTATCGAAGCGCGAAAGCCCGATTTTGACGCTTATATTGATCCACAAAAGCAGTATGCTGATGCAGTCATTGAAGTGTTGCCAACCGAACTCATTCCAGGAGACAATGAGGGCAAGGTTTTGAGAGTGAGGTTGATAATGAAGGAGGGAGTCAAGTTCTTCAACCCAGTCTACTTGTTTGATGAGGGCTCTACCATCTCATGGATACCATGCGGAAGGAAGCTTACTTGCTCTTACCCTGGAATCAAATTCACCTATGGCCCTGACACTTACTATGGCCATGAGGTGTCAGTGTTGGAGATGGATGGACAGTTTGACAGATTAGATGAGCTGATCTATGTAGAAAGCCATCTAAGCAACATCTCCACCAAGTTCTACGGAGAAGTCACGCAACAGATGTTGAAGCATTCCGATTTCCCCGGGAGCAACAACGGGACTGGTCTCTTCCAAACCATCGTTGGATTGAAGATCAGAGACCTCTATGAGCAGCTCATTGCAAGCAAGGCCAAAAGACCAGTAGAAGCAAAAGCTTAA

Protein Analysis

407

Amino Acids

45.17

Weight (kDa)

6.33

Isoelectric Point (pI)

27.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRK PF00485 64 - 262 1.8e-79 Phosphoribulokinase / Uridine kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014052)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32060
fragaria_vesca FvH4_5g23520
malus_domestica MD00G1068500.v1.1 MD06G1023500.v1.1
prunus_persica Prupe.5G024600_v2.0.a1
pyrus_communis pycom06g01910 pycom16g25320
rosa_chinensis RchiOBHm_Chr7g0212791
rosa_laevigata RLG00000002868
rosa_multiflora Rmu_sc0000394.1_g000036
rosa_roxburghii Rroxscaffold_3G00246550
rosa_rugosa Rorug07G0136800
rosa_samantha Rh7AG268300 Rh7BG263700 Rh7CG287300
rosa_wichuraiana Rw7G023180 Rw7G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 572
AccB7I CCANNNNNTGG 1 cut(s) 891
AccI GTMKAC 1 cut(s) 861
AccII CGCG 2 cut(s) 170, 703
AciI CCGC 5 cut(s) 168, 170, 204, 219, 903
AclWI GGATC 3 cut(s) 129, 526, 725
AcoI YGGCCR 4 cut(s) 3, 175, 654, 967
AcsI RAATTY 2 cut(s) 639, 938
AcuI CTGAAG 1 cut(s) 577
AfaI GTAC 2 cut(s) 28, 563
AfiI CCNNNNNNNGG 5 cut(s) 149, 219, 281, 284, 891
AgsI TTSAA 6 cut(s) 37, 545, 764, 853, 1093, 1156
AjnI CCWGG 3 cut(s) 177, 787, 928
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 6 cut(s) 161, 514, 913, 1021, 1180, 1220
AluI AGCT 6 cut(s) 161, 514, 913, 1021, 1180, 1220
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 5 cut(s) 176, 677, 786, 1136, 1158
AlwI GGATC 3 cut(s) 129, 526, 725
Ama87I CYCGRG 2 cut(s) 674, 1109
AoxI GGCC 7 cut(s) 3, 175, 196, 654, 949, 967, 1194
ApeKI GCWGC 2 cut(s) 266, 1177
ApoI RAATTY 2 cut(s) 639, 938
Asp700I GAANNNNTTC 1 cut(s) 783
AspLEI GCGC 2 cut(s) 167, 703
AspS9I GGNCC 3 cut(s) 502, 580, 950
AsuC2I CCSGG 2 cut(s) 1110, 1111
AsuHPI GGTGA 2 cut(s) 202, 934
AvaI CYCGRG 2 cut(s) 674, 1109
AvaII GGWCC 2 cut(s) 502, 580
BaeI ACNNNNGTAYC 2 cut(s) 580, 613
BalI TGGCCA 3 cut(s) 5, 177, 969
BanII GRGCYC 1 cut(s) 881
BarI GAAGNNNNNNTAC 2 cut(s) 898, 930
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 278, 1189
BccI CCATC 6 cut(s) 446, 893, 985, 989, 1047, 1151
BciT130I CCWGG 3 cut(s) 179, 789, 930
BciVI GTATCC 1 cut(s) 888
BcnI CCSGG 2 cut(s) 1110, 1111
BcoDI GTCTC 5 cut(s) 176, 677, 786, 1136, 1158
BfuI GTATCC 1 cut(s) 888
BisI GCNGC 4 cut(s) 168, 204, 267, 1178
BlsI GCNGC 4 cut(s) 169, 205, 268, 1179
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 5 cut(s) 179, 789, 930, 1110, 1111
Bme18I GGWCC 2 cut(s) 502, 580
BmeT110I CYCGRG 2 cut(s) 674, 1109
BmgT120I GGNCC 3 cut(s) 502, 580, 950
BmiI GGNNCC 2 cut(s) 504, 581
BmrFI CCNGG 5 cut(s) 179, 789, 930, 1110, 1111
BmrI ACTGGG 1 cut(s) 851
BmsI GCATC 2 cut(s) 690, 742
BmuI ACTGGG 1 cut(s) 851
BpmI CTGGAG 1 cut(s) 492
BpuMI CCSGG 2 cut(s) 1110, 1111
BsaI GGTCTC 2 cut(s) 1136, 1158
BsaJI CCNNGG 4 cut(s) 168, 928, 1108, 1109
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 281, 311
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 219, 281, 284, 891
Bse1I ACTGG 5 cut(s) 370, 690, 857, 1132, 1205
Bse3DI GCAATG 2 cut(s) 622, 1182
BseBI CCWGG 3 cut(s) 179, 789, 930
BseDI CCNNGG 4 cut(s) 168, 928, 1108, 1109
BseGI GGATG 3 cut(s) 346, 457, 1000
BseLI CCNNNNNNNGG 5 cut(s) 149, 219, 281, 284, 891
BseMI GCAATG 2 cut(s) 622, 1182
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 5 cut(s) 370, 690, 857, 1132, 1205
BseRI GAGGAG 3 cut(s) 136, 253, 276
BseXI GCAGC 2 cut(s) 278, 1189
BsgI GTGCAG 1 cut(s) 33
Bsh1236I CGCG 2 cut(s) 170, 703
BshFI GGCC 7 cut(s) 5, 177, 198, 656, 951, 969, 1196
BsiHKAI GWGCWC 1 cut(s) 230
BsiHKCI CYCGRG 2 cut(s) 674, 1109
BsiSI CCGG 2 cut(s) 213, 1110
BslFI GGGAC 3 cut(s) 593, 662, 1138
BslI CCNNNNNNNGG 5 cut(s) 149, 219, 281, 284, 891
BsmAI GTCTC 5 cut(s) 176, 677, 786, 1136, 1158
BsmFI GGGAC 3 cut(s) 593, 662, 1138
BsmI GAATGC 1 cut(s) 1096
BsnI GGCC 7 cut(s) 5, 177, 198, 656, 951, 969, 1196
Bso31I GGTCTC 2 cut(s) 1136, 1158
BsoBI CYCGRG 2 cut(s) 674, 1109
Bsp1286I GDGCHC 2 cut(s) 230, 881
Bsp143I GATC 5 cut(s) 134, 531, 730, 1023, 1158
BspACI CCGC 5 cut(s) 168, 170, 204, 219, 903
BspANI GGCC 7 cut(s) 5, 177, 198, 656, 951, 969, 1196
BspCNI CTCAG 1 cut(s) 43
BspFNI CGCG 2 cut(s) 170, 703
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 504, 581
BspPI GGATC 3 cut(s) 129, 526, 725
BspQI GCTCTTC 1 cut(s) 218
BspTNI GGTCTC 2 cut(s) 1136, 1158
BsrDI GCAATG 2 cut(s) 622, 1182
BsrI ACTGG 5 cut(s) 370, 690, 857, 1132, 1205
BssECI CCNNGG 4 cut(s) 168, 928, 1108, 1109
BssMI GATC 5 cut(s) 134, 531, 730, 1023, 1158
Bst2UI CCWGG 3 cut(s) 179, 789, 930
Bst4CI ACNGT 4 cut(s) 187, 328, 671, 1002
Bst6I CTCTTC 2 cut(s) 218, 1139
BstC8I GCNNGC 3 cut(s) 163, 551, 1189
BstDEI CTNAG 2 cut(s) 30, 1043
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 3 cut(s) 346, 457, 1000
BstFNI CGCG 2 cut(s) 170, 703
BstHHI GCGC 2 cut(s) 167, 703
BstKTI GATC 5 cut(s) 137, 534, 733, 1026, 1161
BstMAI GTCTC 5 cut(s) 176, 677, 786, 1136, 1158
BstMBI GATC 5 cut(s) 134, 531, 730, 1023, 1158
BstMWI GCNNNNNNNGC 6 cut(s) 11, 167, 225, 520, 1044, 1193
BstNI CCWGG 3 cut(s) 179, 789, 930
BstNSI RCATGY 1 cut(s) 48
BstSCI CCNGG 5 cut(s) 177, 787, 928, 1108, 1109
BstUI CGCG 2 cut(s) 170, 703
BstV1I GCAGC 2 cut(s) 278, 1189
BstX2I RGATCY 2 cut(s) 134, 531
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 2 cut(s) 134, 531
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 7 cut(s) 5, 177, 198, 656, 951, 969, 1196
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 3 cut(s) 346, 457, 1000
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 3 cut(s) 324, 387, 987
Cac8I GCNNGC 3 cut(s) 163, 551, 1189
CciI TCATGA 1 cut(s) 234
CfoI GCGC 2 cut(s) 167, 703
Cfr13I GGNCC 3 cut(s) 502, 580, 950
Cfr42I CCGCGG 1 cut(s) 171
Cfr9I CCCGGG 1 cut(s) 1109
CseI GACGC 1 cut(s) 728
Csp6I GTAC 2 cut(s) 27, 562
CviQI GTAC 2 cut(s) 27, 562
DdeI CTNAG 2 cut(s) 30, 1043
DpnI GATC 5 cut(s) 136, 533, 732, 1025, 1160
DpnII GATC 5 cut(s) 134, 531, 730, 1023, 1158
DrdI GACNNNNNNGTC 1 cut(s) 572
DseDI GACNNNNNNGTC 1 cut(s) 572
EaeI YGGCCR 4 cut(s) 3, 175, 654, 967
Eam1104I CTCTTC 2 cut(s) 218, 1139
EarI CTCTTC 2 cut(s) 218, 1139
Eco24I GRGCYC 1 cut(s) 881
Eco31I GGTCTC 2 cut(s) 1136, 1158
Eco47I GGWCC 2 cut(s) 502, 580
Eco57I CTGAAG 1 cut(s) 577
Eco88I CYCGRG 2 cut(s) 674, 1109
EcoO109I RGGNCCY 1 cut(s) 580
EcoRII CCWGG 3 cut(s) 177, 787, 928
EcoT38I GRGCYC 1 cut(s) 881
FaqI GGGAC 3 cut(s) 593, 662, 1138
FauI CCCGC 2 cut(s) 163, 212
FblI GTMKAC 1 cut(s) 861
Fnu4HI GCNGC 4 cut(s) 168, 204, 267, 1178
FokI GGATG 3 cut(s) 353, 464, 1007
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 4 cut(s) 168, 204, 267, 1178
GlaI GCGC 2 cut(s) 166, 702
GluI GCNGC 4 cut(s) 168, 204, 267, 1178
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 7 cut(s) 5, 177, 198, 656, 951, 969, 1196
HapII CCGG 2 cut(s) 213, 1110
HgaI GACGC 1 cut(s) 728
HhaI GCGC 2 cut(s) 167, 703
Hin6I GCGC 2 cut(s) 165, 701
HinP1I GCGC 2 cut(s) 165, 701
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 2 cut(s) 911, 1218
HinfI GANTC 8 cut(s) 84, 209, 296, 384, 495, 573, 840, 933
HpaII CCGG 2 cut(s) 213, 1110
HphI GGTGA 2 cut(s) 202, 934
Hpy166II GTNNAC 5 cut(s) 19, 246, 494, 665, 862
Hpy188I TCNGA 4 cut(s) 156, 645, 1102, 1163
Hpy188III TCNNGA 5 cut(s) 235, 395, 509, 674, 676
Hpy8I GTNNAC 5 cut(s) 19, 246, 494, 665, 862
HpyAV CCTTC 6 cut(s) 363, 367, 373, 539, 826, 900
HpyCH4III ACNGT 4 cut(s) 187, 328, 671, 1002
HpyCH4V TGCA 5 cut(s) 14, 553, 632, 755, 1187
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 167, 225, 520, 1044, 1193
HpyF3I CTNAG 2 cut(s) 30, 1043
HspAI GCGC 2 cut(s) 165, 701
KspI CCGCGG 1 cut(s) 171
Kzo9I GATC 5 cut(s) 134, 531, 730, 1023, 1158
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 2 cut(s) 263, 1113
Lsp1109I GCAGC 2 cut(s) 278, 1189
LweI GCATC 2 cut(s) 690, 742
MaeIII GTNAC 3 cut(s) 314, 385, 1075
MalI GATC 5 cut(s) 136, 533, 732, 1025, 1160
MboI GATC 5 cut(s) 134, 531, 730, 1023, 1158
MboII GAAGA 4 cut(s) 235, 841, 1126, 1168
MflI RGATCY 2 cut(s) 134, 531
MhlI GDGCHC 2 cut(s) 230, 881
MlsI TGGCCA 3 cut(s) 5, 177, 969
MluCI AATT 2 cut(s) 639, 938
MluNI TGGCCA 3 cut(s) 5, 177, 969
MlyI GAGTC 6 cut(s) 203, 290, 393, 489, 582, 849
MmeI TCCRAC 3 cut(s) 324, 966, 1129
Mox20I TGGCCA 3 cut(s) 5, 177, 969
MroXI GAANNNNTTC 1 cut(s) 783
MscI TGGCCA 3 cut(s) 5, 177, 969
MseI TTAA 2 cut(s) 624, 1222
MslI CAYNNNNRTG 4 cut(s) 233, 326, 489, 764
Msp20I TGGCCA 3 cut(s) 5, 177, 969
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 213, 1110
MspR9I CCNGG 5 cut(s) 179, 789, 930, 1110, 1111
Mva1269I GAATGC 1 cut(s) 1096
MvaI CCWGG 3 cut(s) 179, 789, 930
MvnI CGCG 2 cut(s) 170, 703
MwoI GCNNNNNNNGC 6 cut(s) 11, 167, 225, 520, 1044, 1193
NciI CCSGG 2 cut(s) 1110, 1111
NdeII GATC 5 cut(s) 134, 531, 730, 1023, 1158
NlaIV GGNNCC 2 cut(s) 504, 581
NmeAIII GCCGAG 1 cut(s) 682
NmuCI GTSAC 3 cut(s) 314, 385, 1075
NspI RCATGY 1 cut(s) 48
OliI CACNNNNGTG 1 cut(s) 326
PaeR7I CTCGAG 1 cut(s) 674
PagI TCATGA 1 cut(s) 234
PciSI GCTCTTC 1 cut(s) 218
PctI GAATGC 1 cut(s) 1096
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 2 cut(s) 84, 933
PflFI GACNNNGTC 1 cut(s) 669
PflMI CCANNNNNTGG 1 cut(s) 891
PfoI TCCNGGA 1 cut(s) 787
PkrI GCNGC 4 cut(s) 169, 205, 268, 1179
PleI GAGTC 6 cut(s) 203, 290, 392, 489, 581, 848
PpsI GAGTC 6 cut(s) 203, 290, 392, 489, 581, 848
PpuMI RGGWCCY 1 cut(s) 580
Psp5II RGGWCCY 1 cut(s) 580
Psp6I CCWGG 3 cut(s) 177, 787, 928
PspGI CCWGG 3 cut(s) 177, 787, 928
PspN4I GGNNCC 2 cut(s) 504, 581
PspPI GGNCC 3 cut(s) 502, 580, 950
PspPPI RGGWCCY 1 cut(s) 580
PsuI RGATCY 2 cut(s) 134, 531
PsyI GACNNNGTC 1 cut(s) 669
RsaI GTAC 2 cut(s) 28, 563
RsaNI GTAC 2 cut(s) 27, 562
RseI CAYNNNNRTG 4 cut(s) 233, 326, 489, 764
SacII CCGCGG 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 2 cut(s) 624, 1222
SatI GCNGC 4 cut(s) 168, 204, 267, 1178
Sau3AI GATC 5 cut(s) 134, 531, 730, 1023, 1158
Sau96I GGNCC 3 cut(s) 502, 580, 950
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 6 cut(s) 203, 290, 393, 489, 582, 849
ScrFI CCNGG 5 cut(s) 179, 789, 930, 1110, 1111
SduI GDGCHC 2 cut(s) 230, 881
SfaNI GCATC 2 cut(s) 690, 742
Sfr274I CTCGAG 1 cut(s) 674
Sfr303I CCGCGG 1 cut(s) 171
SgrBI CCGCGG 1 cut(s) 171
SinI GGWCC 2 cut(s) 502, 580
SlaI CTCGAG 1 cut(s) 674
SmaI CCCGGG 1 cut(s) 1111
SmiMI CAYNNNNRTG 4 cut(s) 233, 326, 489, 764
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
Sse9I AATT 2 cut(s) 639, 938
SsiI CCGC 5 cut(s) 168, 170, 204, 219, 903
StyD4I CCNGG 5 cut(s) 177, 787, 928, 1108, 1109
TaaI ACNGT 4 cut(s) 187, 328, 671, 1002
TaqI TCGA 2 cut(s) 675, 696
TasI AATT 2 cut(s) 639, 938
TatI WGTACW 1 cut(s) 26
TauI GCSGC 2 cut(s) 170, 206
TfiI GAWTC 2 cut(s) 84, 933
Tru1I TTAA 2 cut(s) 624, 1222
Tru9I TTAA 2 cut(s) 624, 1222
TscAI CASTG 3 cut(s) 331, 394, 987
TseFI GTSAC 3 cut(s) 314, 385, 1075
TseI GCWGC 2 cut(s) 266, 1177
Tsp45I GTSAC 3 cut(s) 314, 385, 1075
TspDTI ATGAA 2 cut(s) 223, 845
TspGWI ACGGA 1 cut(s) 1083
TspMI CCCGGG 1 cut(s) 1109
TspRI CASTG 3 cut(s) 331, 394, 987
Tth111I GACNNNGTC 1 cut(s) 669
Van91I CCANNNNNTGG 1 cut(s) 891
VpaK11BI GGWCC 2 cut(s) 502, 580
XapI RAATTY 2 cut(s) 639, 938
XceI RCATGY 1 cut(s) 48
XcmI CCANNNNNNNNNTGG 1 cut(s) 1145
XhoI CTCGAG 1 cut(s) 674
XmaI CCCGGG 1 cut(s) 1109
XmiI GTMKAC 1 cut(s) 861
XmnI GAANNNNTTC 1 cut(s) 783
ZrmI AGTACT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.