AT1G35420

Dienelactone hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011294
Physical Location & Seq
Forward (+)
13026206 .. 13027691
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G35420.3

Sequence Viewer

Length: 933 bp
ATGGCTGCATCGTTAGCCTCTCTTTCCTCCTTAGCTAAAGCCGGAGCACCACGGTCACTGTTCAACCGCCATGGCAGCCACCGCCTTCCTCCTCCACAAATCATACCTCTTGCTTCCTCTATCAAGAAACATATCACGACGAGCTTCTATAGGAGAGGAAGTTCTCTGAAGAACATTTATGCCCTCGAGACCTCTGACGGCGCCATTAACGTGGAGGTCGATGATGATGAGGAAGAAGAAGCGTGTGAGCTTGTTAACGGAACAGAAGTGTCCGTTGACGGTGTTGAAGGTTATCTTTTAACGGCGGTGAAGAACAATAACGGAACTGGATTGCTTCTACTCTCTGATGTCTTTGGCTTTCAAGATTCAGCTACACGCGATTTCGCTTACCGTGTGGCTTGCAATGGCTATAACGTTCTTGTCCCGGACTTGTTTCGTGGAGATCCATGGTCGAAAAACCGACCAAAGTCCGAATACGAGGAATGGAGAAGAGGACATGACCCTAACCGTATCAGACAAGACACTACTACCTTCACAAAATGGATGGTTGAGGAATTTGCAGCGGCTGGAATCTCCAAGAAGCTAGGAGTGATGGGGTTCTGTTTTGGCGGTGGACGTGTGGTTGACGTGTTAGCCACGGATGAGAGCGGTTACTTTAGCACCGGTGTCTCGTTCTACGGTACGAGAATAGACTCGGCGGTGGCGGGAGATGTGAAAGTGCCAGTCTTGTTTATTGCTGGAGATAGAGACCCGCTTTGTGAAGTGAAGGGTTTGTATGAAATTGAGGAGAAGATTGGTGAAGGTTCAAAGGTTGTTGTGTATGAAGGAAGAGGTCACGGCTTTGTTCACCGGCCCGAGACACCCGAAGATGATCGAGACGCTGAGGAAGCGTTTGCTCTCATGAGAAATTGGTTACATCATCATTTGATTTGA

Protein Analysis

310

Amino Acids

34.17

Weight (kDa)

5.27

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 60 - 272 3.3e-29 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G35420 AT1G35420 AT1G35420
fragaria_vesca FvH4_5g31620 FvH4_5g31620
malus_domestica MD08G1217800.v1.1
prunus_persica Prupe.1G555600_v2.0.a1
pyrus_communis pycom08g18880
rosa_chinensis RchiOBHm_Chr7g0227151
rosa_laevigata RLG00000001664
rosa_multiflora Rmu_co8431879.1_g000001 Rmu_sc0003153.1_g000019
rosa_roxburghii Rroxscaffold_3G00232270
rosa_rugosa Rorug07G0241000 Rorug07G0241000
rosa_samantha Rh7AG390900 Rh7BG375900 Rh7CG409900 Rh7DG388200
rosa_wichuraiana Rw7G032700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AccBSI CCGCTC 1 cut(s) 648
AccII CGCG 1 cut(s) 378
AciI CCGC 9 cut(s) 67, 82, 305, 563, 609, 648, 698, 704, 752
AclI AACGTT 1 cut(s) 414
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 1 cut(s) 682
AflIII ACRYGT 2 cut(s) 616, 627
AgeI ACCGGT 1 cut(s) 662
AgsI TTSAA 4 cut(s) 64, 287, 362, 807
AjiI CACGTC 2 cut(s) 617, 628
AloI GAACNNNNNNTCC 2 cut(s) 145, 177
AluBI AGCT 5 cut(s) 35, 144, 250, 371, 583
AluI AGCT 5 cut(s) 35, 144, 250, 371, 583
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 5 cut(s) 182, 673, 741, 851, 870
AlwI GGATC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 2 cut(s) 185, 854
AoxI GGCC 1 cut(s) 851
ApeKI GCWGC 3 cut(s) 5, 75, 560
ApoI RAATTY 1 cut(s) 554
AsiGI ACCGGT 1 cut(s) 662
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 1 cut(s) 852
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 3 cut(s) 319, 809, 839
AvaI CYCGRG 2 cut(s) 185, 854
BanI GGYRCC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 49
BbvCI CCTCAGC 1 cut(s) 882
BbvI GCAGC 2 cut(s) 87, 572
BccI CCATC 2 cut(s) 538, 586
BceAI ACGGC 3 cut(s) 214, 318, 853
BcnI CCSGG 1 cut(s) 425
BcoDI GTCTC 5 cut(s) 182, 673, 741, 851, 870
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 148
BfoI RGCGCY 1 cut(s) 204
BisI GCNGC 4 cut(s) 6, 76, 561, 564
BlsI GCNGC 4 cut(s) 7, 77, 562, 565
Bme1390I CCNGG 1 cut(s) 425
BmeT110I CYCGRG 2 cut(s) 185, 854
BmgBI CACGTC 2 cut(s) 617, 628
BmgT120I GGNCC 1 cut(s) 852
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 17
BoxI GACNNNNGTC 1 cut(s) 466
BpmI CTGGAG 1 cut(s) 759
Bpu10I CCTNAGC 2 cut(s) 31, 882
BpuMI CCSGG 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 2 cut(s) 182, 741
BsaJI CCNNGG 4 cut(s) 50, 70, 446, 636
BsaWI WCCGGW 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bse118I RCCGGY 2 cut(s) 662, 849
Bse1I ACTGG 2 cut(s) 331, 722
Bse3DI GCAATG 1 cut(s) 409
BseDI CCNNGG 4 cut(s) 50, 70, 446, 636
BseGI GGATG 2 cut(s) 549, 646
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 1 cut(s) 873
BseNI ACTGG 2 cut(s) 331, 722
BseRI GAGGAG 2 cut(s) 81, 800
BseXI GCAGC 2 cut(s) 87, 572
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 853
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 1 cut(s) 662
BsiHKAI GWGCWC 1 cut(s) 49
BsiHKCI CYCGRG 2 cut(s) 185, 854
BsiSI CCGG 4 cut(s) 42, 425, 663, 850
BslFI GGGAC 1 cut(s) 407
BsmAI GTCTC 5 cut(s) 182, 673, 741, 851, 870
BsmBI CGTCTC 1 cut(s) 870
BsmFI GGGAC 1 cut(s) 407
BsnI GGCC 1 cut(s) 853
Bso31I GGTCTC 2 cut(s) 182, 741
BsoBI CYCGRG 2 cut(s) 185, 854
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 442, 871
Bsp19I CCATGG 2 cut(s) 70, 446
BspACI CCGC 9 cut(s) 67, 82, 305, 563, 609, 648, 698, 704, 752
BspANI GGCC 1 cut(s) 853
BspCNI CTCAG 1 cut(s) 874
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 1 cut(s) 202
BspPI GGATC 1 cut(s) 437
BspT107I GGYRCC 1 cut(s) 200
BspTNI GGTCTC 2 cut(s) 182, 741
BsrBI CCGCTC 1 cut(s) 648
BsrDI GCAATG 1 cut(s) 409
BsrFI RCCGGY 2 cut(s) 662, 849
BsrI ACTGG 2 cut(s) 331, 722
BssAI RCCGGY 2 cut(s) 662, 849
BssECI CCNNGG 4 cut(s) 50, 70, 446, 636
BssMI GATC 2 cut(s) 442, 871
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 2 cut(s) 70, 446
Bst4CI ACNGT 6 cut(s) 54, 60, 281, 392, 509, 680
Bst6I CTCTTC 2 cut(s) 484, 823
BstACI GRCGYC 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 2 cut(s) 31, 882
BstDSI CCRYGG 4 cut(s) 50, 70, 446, 636
BstF5I GGATG 2 cut(s) 549, 646
BstFNI CGCG 1 cut(s) 378
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 2 cut(s) 445, 874
BstMAI GTCTC 5 cut(s) 182, 673, 741, 851, 870
BstMBI GATC 2 cut(s) 442, 871
BstMWI GCNNNNNNNGC 4 cut(s) 14, 75, 81, 887
BstPAI GACNNNNGTC 1 cut(s) 466
BstSCI CCNGG 1 cut(s) 423
BstSFI CTRYAG 1 cut(s) 148
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 2 cut(s) 87, 572
BstX2I RGATCY 1 cut(s) 442
BstXI CCANNNNNNTGG 1 cut(s) 211
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 1 cut(s) 853
BtgI CCRYGG 4 cut(s) 50, 70, 446, 636
BtrI CACGTC 2 cut(s) 617, 628
BtsCI GGATG 2 cut(s) 549, 646
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 1 cut(s) 400
CaiI CAGNNNCTG 1 cut(s) 566
CciI TCATGA 1 cut(s) 900
CfoI GCGC 1 cut(s) 203
Cfr10I RCCGGY 2 cut(s) 662, 849
Cfr13I GGNCC 1 cut(s) 852
CseI GACGC 1 cut(s) 887
Csp6I GTAC 1 cut(s) 681
CspAI ACCGGT 1 cut(s) 662
CviAII CATG 4 cut(s) 71, 447, 497, 901
CviQI GTAC 1 cut(s) 681
DdeI CTNAG 2 cut(s) 31, 882
DinI GGCGCC 1 cut(s) 202
DpnI GATC 2 cut(s) 444, 873
DpnII GATC 2 cut(s) 442, 871
Eam1104I CTCTTC 2 cut(s) 484, 823
EarI CTCTTC 2 cut(s) 484, 823
Eco130I CCWWGG 2 cut(s) 70, 446
Eco31I GGTCTC 2 cut(s) 182, 741
Eco57I CTGAAG 1 cut(s) 188
Eco88I CYCGRG 2 cut(s) 185, 854
EcoT14I CCWWGG 2 cut(s) 70, 446
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 70, 446
Esp3I CGTCTC 1 cut(s) 870
FaeI CATG 4 cut(s) 74, 450, 500, 904
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 1 cut(s) 407
FatI CATG 4 cut(s) 70, 446, 496, 900
FauI CCCGC 2 cut(s) 697, 759
Fnu4HI GCNGC 4 cut(s) 6, 76, 561, 564
FokI GGATG 2 cut(s) 556, 653
Fsp4HI GCNGC 4 cut(s) 6, 76, 561, 564
FspBI CTAG 1 cut(s) 584
GlaI GCGC 1 cut(s) 202
GluI GCNGC 4 cut(s) 6, 76, 561, 564
GsuI CTGGAG 1 cut(s) 759
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 1 cut(s) 853
HapII CCGG 4 cut(s) 42, 425, 663, 850
HgaI GACGC 1 cut(s) 887
HhaI GCGC 1 cut(s) 203
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 4 cut(s) 74, 450, 500, 904
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HincII GTYRAC 3 cut(s) 256, 277, 625
HindII GTYRAC 3 cut(s) 256, 277, 625
HinfI GANTC 3 cut(s) 365, 570, 692
HpaI GTTAAC 1 cut(s) 256
HpaII CCGG 4 cut(s) 42, 425, 663, 850
HphI GGTGA 3 cut(s) 319, 809, 839
Hpy166II GTNNAC 5 cut(s) 256, 277, 614, 625, 847
Hpy188I TCNGA 5 cut(s) 168, 196, 346, 472, 515
Hpy188III TCNNGA 6 cut(s) 124, 136, 187, 362, 875, 901
Hpy8I GTNNAC 5 cut(s) 256, 277, 614, 625, 847
Hpy99I CGWCG 1 cut(s) 142
HpyAV CCTTC 6 cut(s) 95, 281, 541, 760, 794, 818
HpyCH4III ACNGT 6 cut(s) 54, 60, 281, 392, 509, 680
HpyCH4IV ACGT 4 cut(s) 210, 414, 616, 627
HpyCH4V TGCA 3 cut(s) 8, 402, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 75, 81, 887
HpyF3I CTNAG 2 cut(s) 31, 882
HpySE526I ACGT 4 cut(s) 210, 414, 616, 627
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 4 cut(s) 74, 450, 500, 904
HspAI GCGC 1 cut(s) 201
KasI GGCGCC 1 cut(s) 200
KspAI GTTAAC 1 cut(s) 256
Kzo9I GATC 2 cut(s) 442, 871
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 8 cut(s) 55, 312, 438, 552, 676, 723, 735, 863
Lsp1109I GCAGC 2 cut(s) 87, 572
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 584
MaeII ACGT 4 cut(s) 210, 414, 616, 627
MaeIII GTNAC 4 cut(s) 54, 650, 833, 912
MalI GATC 2 cut(s) 444, 873
MbiI CCGCTC 1 cut(s) 648
MboI GATC 2 cut(s) 442, 871
MboII GAAGA 8 cut(s) 181, 245, 248, 322, 501, 802, 840, 878
MflI RGATCY 1 cut(s) 442
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 3 cut(s) 554, 780, 907
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 1 cut(s) 686
MseI TTAA 3 cut(s) 207, 255, 299
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 4 cut(s) 42, 425, 663, 850
MspR9I CCNGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 4 cut(s) 14, 75, 81, 887
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 1 cut(s) 425
NcoI CCATGG 2 cut(s) 70, 446
NdeII GATC 2 cut(s) 442, 871
NlaIII CATG 4 cut(s) 74, 450, 500, 904
NlaIV GGNNCC 1 cut(s) 202
NmeAIII GCCGAG 1 cut(s) 674
NmuCI GTSAC 2 cut(s) 54, 833
PaeR7I CTCGAG 1 cut(s) 185
PagI TCATGA 1 cut(s) 900
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 2 cut(s) 365, 570
PfoI TCCNGGA 1 cut(s) 423
PinAI ACCGGT 1 cut(s) 662
PkrI GCNGC 4 cut(s) 7, 77, 562, 565
PleI GAGTC 1 cut(s) 686
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 686
PshAI GACNNNNGTC 1 cut(s) 466
Psp1406I AACGTT 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 1 cut(s) 852
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 1 cut(s) 682
RsaNI GTAC 1 cut(s) 681
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 3 cut(s) 207, 255, 299
SatI GCNGC 4 cut(s) 6, 76, 561, 564
Sau3AI GATC 2 cut(s) 442, 871
Sau96I GGNCC 1 cut(s) 852
SchI GAGTC 1 cut(s) 686
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 1 cut(s) 49
SfaNI GCATC 1 cut(s) 17
SfcI CTRYAG 1 cut(s) 148
SfoI GGCGCC 1 cut(s) 202
Sfr274I CTCGAG 1 cut(s) 185
SgrAI CRCCGGYG 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 185
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 3 cut(s) 554, 780, 907
SsiI CCGC 9 cut(s) 67, 82, 305, 563, 609, 648, 698, 704, 752
SspDI GGCGCC 1 cut(s) 200
SspMI CTAG 1 cut(s) 584
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 2 cut(s) 70, 446
TaaI ACNGT 6 cut(s) 54, 60, 281, 392, 509, 680
TaiI ACGT 4 cut(s) 213, 417, 619, 630
TaqI TCGA 4 cut(s) 186, 219, 452, 874
TasI AATT 3 cut(s) 554, 780, 907
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 2 cut(s) 365, 570
Tru1I TTAA 3 cut(s) 207, 255, 299
Tru9I TTAA 3 cut(s) 207, 255, 299
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 2 cut(s) 54, 833
TseI GCWGC 3 cut(s) 5, 75, 560
Tsp45I GTSAC 2 cut(s) 54, 833
TspDTI ATGAA 2 cut(s) 792, 837
TspGWI ACGGA 4 cut(s) 262, 273, 336, 653
TspRI CASTG 1 cut(s) 63
XapI RAATTY 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 185
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.