Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014494

Physical Location & Seq

Genomic Coordinates Forward (+)

16970214 .. 16974753

Gene Structure 4540 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G44900.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2811 bp

ATGGCGGGAGAAAATTCCGATAACGAGCCGTCTTCACCAGCTTCACCTTCTTCCGCTGGATTCAACACAGATCAGTTACCAATCAGTACAAGCCAAAACTCCGAAAATTTCTCCGATGAAGAAGAAGCAGCTGTAGATACCCAAGTCATTAGAGATGAGCCTGACGAAGCGGAGGATGAAGAAGAAGAAGAAGGAGAAGATCTCTTCAATGATACTTTTATGAACGATTATCGGAAGATGGATGAGAATGATCAGTATGAGTCTAATGGAATAGATGATTCCGTCGATGATGAAAGAGATTTAGGTCAGGCTATGCTTGACCGTCGTGCTGCTGACGCTGATCTTGATGCTCGTGAAAATCGACTTGCGAATCGCAAGCTTCCTCATCTTCTTCACGATAACGATTCAGATGATTGGAACTATAGACCTTCGAAGAGGTCTCGAACTACGGTTCCACCGAGAGGTAATGGTGGTGATCCTGATGGGAATCCTCCAAGTTCGCCAGGAGTGTCACAGCCAGATATCTCTATGACTGATCAAACTGATGATTATCAAGATGAGGATGATAATGATGATGAAGCAGAGTTTGAGATGTATAGGATTCAAGGAACTCTGAGGGAATGGGTTATGAGAGATGAAGTGAGACGGTTTATTGCTAAGAAGTTTAAAGACTTTTTGCTTACGTATGTTAAACCAAAGAATGAGAATGGAGATATTGAATATGTTCGTTTAATTAACGAAATGGTTTCAGCTAATAAGTGCAGTCTGGAAATTGATTACAAAGAATTCATACACGTCCACCCGAATATTGCAATCTGGCTTGCGGATGCTCCACAACCTGTGCTTGAAGTAATGGAGGAAGTTTCTGAAAAGGTCATCTTTGATCTGCATCCAAACTACAAGAATATTCACACGAAGATATATGTTCGTGTTACCAACCTACCAGTTAATGATCAGATTCGGAACATCAGGCAGATTCATCTTAACACGATGATCCGTATCGGTGGAGTTGTCACTAGGCGTTCTGGGGTCTTTCCTCAGCTGCAGCAAGTGAAATATGATTGTAACAAGTGTGGAGCAGTTTTGGGGCCTTTCTTCCAAAACTCTTATTCAGAAGTCAAGGTCGGTTCTTGTTCTGAATGCCAGTCAAAAGGACCGTTTACTGTTAATGTCGAACAGACAATATACAGGAACTATCAGAAGCTTACAATCCAAGAGAGTCCAGGAACAGTGCCTGCTGGACGACTTCCAAGACACAAAGAAGTTATCCTGCTGAATGACCTTATTGATTGCGCTCGTCCTGGGGAAGAAATTGAGGTTACTGGCATATATACCAACAACTTTGACCTATCTTTGAACACAAAGAATGGGTTTCCAGTCTTTGCCACTGTGGTGGAAGCTAACTATGTTACAAAGAAACAAGACCTCTTCTCTGCGTACAAGCTAACCCAAGAAGACAAGACACAAATCGAAGAGCTATCCAAGGATCCACGAATAGTTGAACGTATCATTAAGTCAATTGCTCCGTCAATATATGGCCACGAAGATATCAAAACAGCTCTTGCTCTTGCTATGTTTGGTGGCCAAGAGAAAAATATCAAAGGGAAACACAGATTGCGTGGAGATATTAATGTACTTTTACTAGGTGATCCAGGAACAGCAAAATCACAATTTCTGAAGTATGTTGAAAAAACTGGTCAAAGAGCTGTCTACACGACTGGAAAGGGAGCTTCTGCTGTTGGTCTAACCGCAGCAGTACACAAAGATCCAGTTACAAGGGAGTGGACGCTTGAAGGAGGGGCTCTTGTACTTGCTGACCGAGGAATTTGTCTTATCGATGAGTTTGACAAGATGAACGACCAGGACAGGGTGAGTATCCATGAAGCCATGGAACAACAGAGTATTAGTATATCGAAGGCAGGAATAGTTACTTCTCTTCAAGCTCGTTGCTCTGTTATTGCTGCAGCTAATCCAGTTGGTGGAAGGTATGATTCATCGAAATCATTTGCACAAAATGTCGAGTTGACAGATCCGATCCTTTCTCGTTTTGATATCTTGTGTGTTGTCAAGGATGTGGTTGACCCAGTTACAGATGAAATGCTAGCTGAATTTGTTGTCAATAGCCACTTCAAGTCACAACCCAAAGGTGGTAAGATGGAAGATAGCGATCCCGAAGATGGCATTCAGGGATCTAGCGGGTCAACTGATCCGGAGGTCCTTCCTCAGAACCTGCTGAAGAAATACTTAACATACTCGAAGTTGTATGTATTCCCAAAACTGGGTGAACTAGATGCCAAGAAGTTAGAGACTGTTTATGCTAATCTAAGACGAGAATCAATGAACGGACAAGGAGTTTCTATAGCGACAAGGCACCTTGAGTCCATGATCCGAATGTCTGAAGCACATGCTAGGATGCACCTTAGGCAATATGTCACAGAAGAGGATGTTAATATGGCCATTCGAGTCCTACTTGACTCTTTCATATCAACCCAGAAATTTGGTGTCCAGAGAACTCTAAGAGAGAGTTTCAAACGGTACATTACATACAAAAAGGACTTCAACTCATTGCTGCTCGTTCTTCTCAAAGAGCTCGTAAAGAACGCCTTGAAGTTTGAAGAAATCATTTCAGGATCGAACTCAGGACTACCTACAATCGAGGTTAAGATAGAGGAACTGCAGACAAAGGCCAAGGAATACGACATTGCAGATCTACGACCCTTCTTTTCAAGCACAGATTTTTCCAAAGCTCACTTCGAGTTGGATCATGGCCGAGGAATGATCAAGTGTCCAAAAAGGCTGATCACTTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000347 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010015 GO:0010073 GO:0010075 GO:0010082 GO:0010154 GO:0016043 GO:0022414 GO:0022622 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0040008 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048364 GO:0048507 GO:0048509 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051239 GO:0051276 GO:0061458 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0099402 GO:1901360 GO:1901576 GO:1905392 GO:2000026 GO:2000280

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM2_N MCM_N MCM_OB

Protein Analysis

936

Amino Acids

105.58

Weight (kDa)

5.0

Isoelectric Point (pI)

42.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM2_N	PF12619	40 - 138	5.7e-15	Mini-chromosome maintenance protein 2
MCM_N	PF14551	218 - 311	1.4e-16	MCM N-terminal domain
MCM_OB	PF17207	317 - 444	1.2e-35	MCM OB domain
MCM	PF00493	485 - 707	2.4e-104	MCM P-loop domain
AAA_5	PF07728	542 - 661	1.8e-06	AAA domain (dynein-related subfamily)
Mg_chelatase	PF01078	596 - 687	9.7e-07	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	742 - 825	1.9e-25	MCM AAA-lid domain
WHD_MCM2	PF23669	849 - 925	3.6e-24	DNA replication licensing factor MCM2-like, winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44900 AT1G44900
fragaria_vesca	FvH4_4g22180
malus_domestica	MD13G1134100.v1.1 MD16G1144700.v1.1
prunus_persica	Prupe.1G211500_v2.0.a1
pyrus_communis	pycom13g11680 pycom16g11400
rosa_chinensis	RchiOBHm_Chr4g0428411
rosa_laevigata	RLG00000007153
rosa_multiflora	Rmu_sc0004698.1_g000003
rosa_roxburghii	Rroxscaffold_5G00370080
rosa_rugosa	Rorug04G0225200
rosa_samantha	Rh4BG288100 Rh4CG303800 Rh4DG285200
rosa_wichuraiana	Rw4G024520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2238
AccB1I	GGYRCC	1 cut(s)	2370
AccB7I	CCANNNNNTGG	1 cut(s)	1979
AccI	GTMKAC	1 cut(s)	1710
AccIII	TCCGGA	1 cut(s)	2209
AciI	CCGC	6 cut(s)	5, 54, 170, 824, 1749, 2196
AcoI	YGGCCR	4 cut(s)	1537, 1582, 2454, 2767
AcsI	RAATTY	6 cut(s)	13, 106, 785, 1824, 2108, 2495
AcuI	CTGAAG	3 cut(s)	1697, 2255, 2418
AfaI	GTAC	6 cut(s)	88, 1439, 1635, 1758, 1809, 2537
AfiI	CCNNNNNNNGG	7 cut(s)	461, 1867, 1979, 2147, 2177, 2278, 2279
AflIII	ACRYGT	1 cut(s)	793
AjiI	CACGTC	1 cut(s)	796
AjnI	CCWGG	5 cut(s)	502, 1222, 1300, 1651, 1860
AjuI	GAANNNNNNNTTGG	2 cut(s)	2735, 2767
AleI	CACNNNNGTG	1 cut(s)	1391
AloI	GAACNNNNNNTCC	2 cut(s)	436, 468
Alw21I	GWGCWC	1 cut(s)	2592
Alw26I	GTCTC	3 cut(s)	444, 637, 2300
AlwNI	CAGNNNCTG	2 cut(s)	1235, 2230
Aor13HI	TCCGGA	1 cut(s)	2209
AoxI	GGCC	6 cut(s)	1088, 1537, 1582, 2454, 2685, 2767
ApeKI	GCWGC	8 cut(s)	128, 329, 1042, 1045, 1751, 1961, 1964, 2569
ApoI	RAATTY	6 cut(s)	13, 106, 785, 1824, 2108, 2495
AseI	ATTAAT	1 cut(s)	1629
Asp700I	GAANNNNTTC	1 cut(s)	723
AspLEI	GCGC	1 cut(s)	1295
AspS9I	GGNCC	3 cut(s)	1088, 1154, 2215
AsuHPI	GGTGA	6 cut(s)	27, 36, 485, 1658, 1882, 2294
AsuII	TTCGAA	1 cut(s)	431
AsuNHI	GCTAGC	1 cut(s)	2101
AvaII	GGWCC	2 cut(s)	1154, 2215
AxyI	CCTNAGG	1 cut(s)	2420
BaeI	ACNNNNGTAYC	2 cut(s)	129, 162
BalI	TGGCCA	3 cut(s)	1539, 1584, 2456
BamHI	GGATCC	1 cut(s)	1486
BanI	GGYRCC	1 cut(s)	2370
BanII	GRGCYC	2 cut(s)	1804, 2592
BauI	CACGAG	1 cut(s)	351
BbsI	GAAGAC	2 cut(s)	24, 1461
Bbv12I	GWGCWC	1 cut(s)	2592
BbvCI	CCTCAGC	1 cut(s)	1038
BbvI	GCAGC	8 cut(s)	140, 316, 1029, 1057, 1763, 1948, 1976, 2556
BccI	CCATC	4 cut(s)	232, 476, 2149, 2171
BceAI	ACGGC	1 cut(s)	13
BciT130I	CCWGG	5 cut(s)	504, 1224, 1302, 1653, 1862
BciVI	GTATCC	1 cut(s)	1886
BclI	TGATCA	5 cut(s)	250, 535, 952, 2778, 2799
BcoDI	GTCTC	3 cut(s)	444, 637, 2300
BfaI	CTAG	6 cut(s)	1017, 1643, 2102, 2193, 2288, 2409
BfmI	CTRYAG	6 cut(s)	132, 421, 1043, 1962, 2358, 2675
BfuAI	ACCTGC	1 cut(s)	2238
BfuI	GTATCC	1 cut(s)	1886
BglII	AGATCT	2 cut(s)	199, 2707
BisI	GCNGC	8 cut(s)	129, 330, 1043, 1046, 1752, 1962, 1965, 2570
BlsI	GCNGC	8 cut(s)	130, 331, 1044, 1047, 1753, 1963, 1966, 2571
Bme1390I	CCNGG	5 cut(s)	504, 1224, 1302, 1653, 1862
Bme18I	GGWCC	2 cut(s)	1154, 2215
BmgBI	CACGTC	1 cut(s)	796
BmgT120I	GGNCC	3 cut(s)	1088, 1154, 2215
BmiI	GGNNCC	4 cut(s)	453, 1089, 1488, 2372
BmrFI	CCNGG	5 cut(s)	504, 1224, 1302, 1653, 1862
BmrI	ACTGGG	2 cut(s)	2078, 2288
BmsI	GCATC	5 cut(s)	337, 817, 898, 2281, 2403
BmtI	GCTAGC	1 cut(s)	2105
BmuI	ACTGGG	2 cut(s)	2078, 2288
BpiI	GAAGAC	2 cut(s)	24, 1461
BplI	GAGNNNNNCTC	2 cut(s)	186, 218
Bpu10I	CCTNAGC	1 cut(s)	1038
Bpu14I	TTCGAA	1 cut(s)	431
BpuEI	CTTGAG	1 cut(s)	2396
Bsa29I	ATCGAT	1 cut(s)	1836
BsaAI	YACGTR	1 cut(s)	684
BsaBI	GATNNNNATC	5 cut(s)	486, 549, 888, 998, 2166
BsaI	GGTCTC	1 cut(s)	444
BsaJI	CCNNGG	6 cut(s)	1301, 1482, 1819, 1887, 2688, 2770
BsaWI	WCCGGW	1 cut(s)	2209
Bsc4I	CCNNNNNNNGG	7 cut(s)	461, 1867, 1979, 2147, 2177, 2278, 2279
Bse21I	CCTNAGG	1 cut(s)	2420
Bse3DI	GCAATG	2 cut(s)	2564, 2700
Bse8I	GATNNNNATC	5 cut(s)	486, 549, 888, 998, 2166
BseAI	TCCGGA	1 cut(s)	2209
BseBI	CCWGG	5 cut(s)	504, 1224, 1302, 1653, 1862
BseCI	ATCGAT	1 cut(s)	1836
BseDI	CCNNGG	6 cut(s)	1301, 1482, 1819, 1887, 2688, 2770
BseGI	GGATG	8 cut(s)	181, 247, 568, 832, 889, 2077, 2418, 2449
BseJI	GATNNNNATC	5 cut(s)	486, 549, 888, 998, 2166
BseLI	CCNNNNNNNGG	7 cut(s)	461, 1867, 1979, 2147, 2177, 2278, 2279
BseMI	GCAATG	2 cut(s)	2564, 2700
BseMII	CTCAG	4 cut(s)	605, 1052, 2237, 2652
BseXI	GCAGC	8 cut(s)	140, 316, 1029, 1057, 1763, 1948, 1976, 2556
BsgI	GTGCAG	1 cut(s)	781
BshFI	GGCC	6 cut(s)	1090, 1539, 1584, 2456, 2687, 2769
BshNI	GGYRCC	1 cut(s)	2370
BshVI	ATCGAT	1 cut(s)	1836
BsiHKAI	GWGCWC	1 cut(s)	2592
BsiSI	CCGG	1 cut(s)	2210
BslI	CCNNNNNNNGG	7 cut(s)	461, 1867, 1979, 2147, 2177, 2278, 2279
BsmAI	GTCTC	3 cut(s)	444, 637, 2300
BsmBI	CGTCTC	1 cut(s)	637
BsmI	GAATGC	2 cut(s)	1145, 2181
BsnI	GGCC	6 cut(s)	1090, 1539, 1584, 2456, 2687, 2769
Bso31I	GGTCTC	1 cut(s)	444
Bsp119I	TTCGAA	1 cut(s)	431
Bsp1286I	GDGCHC	2 cut(s)	1804, 2592
Bsp13I	TCCGGA	1 cut(s)	2209
Bsp19I	CCATGG	1 cut(s)	1887
BspACI	CCGC	6 cut(s)	5, 54, 170, 824, 1749, 2196
BspANI	GGCC	6 cut(s)	1090, 1539, 1584, 2456, 2687, 2769
BspCNI	CTCAG	4 cut(s)	606, 1051, 2236, 2651
BspDI	ATCGAT	1 cut(s)	1836
BspEI	TCCGGA	1 cut(s)	2209
BspLI	GGNNCC	4 cut(s)	453, 1089, 1488, 2372
BspMAI	CTGCAG	3 cut(s)	1047, 1966, 2679
BspMI	ACCTGC	1 cut(s)	2238
BspOI	GCTAGC	1 cut(s)	2105
BspQI	GCTCTTC	1 cut(s)	1467
BspT104I	TTCGAA	1 cut(s)	431
BspT107I	GGYRCC	1 cut(s)	2370
BspTNI	GGTCTC	1 cut(s)	444
BsrDI	GCAATG	2 cut(s)	2564, 2700
BssECI	CCNNGG	6 cut(s)	1301, 1482, 1819, 1887, 2688, 2770
BssSI	CACGAG	1 cut(s)	351
BssT1I	CCWWGG	3 cut(s)	1482, 1887, 2688
Bst2BI	CACGAG	1 cut(s)	351
Bst2UI	CCWGG	5 cut(s)	504, 1224, 1302, 1653, 1862
Bst4CI	ACNGT	9 cut(s)	323, 451, 648, 1158, 1165, 1231, 1390, 2311, 2535
Bst6I	CTCTTC	6 cut(s)	209, 428, 1433, 1467, 1941, 2433
BstBAI	YACGTR	1 cut(s)	684
BstBI	TTCGAA	1 cut(s)	431
BstC8I	GCNNGC	4 cut(s)	377, 822, 1236, 2103
BstDEI	CTNAG	8 cut(s)	614, 657, 1038, 2223, 2324, 2420, 2516, 2638
BstDSI	CCRYGG	1 cut(s)	1887
BstF5I	GGATG	8 cut(s)	181, 247, 568, 832, 889, 2077, 2418, 2449
BstHHI	GCGC	1 cut(s)	1295
BstMAI	GTCTC	3 cut(s)	444, 637, 2300
BstMWI	GCNNNNNNNGC	2 cut(s)	335, 2422
BstNI	CCWGG	5 cut(s)	504, 1224, 1302, 1653, 1862
BstNSI	RCATGY	1 cut(s)	2408
BstSCI	CCNGG	5 cut(s)	502, 1222, 1300, 1651, 1860
BstSFI	CTRYAG	6 cut(s)	132, 421, 1043, 1962, 2358, 2675
BstSNI	TACGTA	1 cut(s)	684
BstV1I	GCAGC	8 cut(s)	140, 316, 1029, 1057, 1763, 1948, 1976, 2556
BstV2I	GAAGAC	2 cut(s)	24, 1461
BstX2I	RGATCY	6 cut(s)	199, 1486, 1765, 2029, 2189, 2707
BstXI	CCANNNNNNTGG	2 cut(s)	1393, 2498
BstYI	RGATCY	6 cut(s)	199, 1486, 1765, 2029, 2189, 2707
Bsu15I	ATCGAT	1 cut(s)	1836
Bsu36I	CCTNAGG	1 cut(s)	2420
BsuI	GTATCC	1 cut(s)	1886
BsuRI	GGCC	6 cut(s)	1090, 1539, 1584, 2456, 2687, 2769
BsuTUI	ATCGAT	1 cut(s)	1836
BtgI	CCRYGG	1 cut(s)	1887
BtrI	CACGTC	1 cut(s)	796
BtsCI	GGATG	8 cut(s)	181, 247, 568, 832, 889, 2077, 2418, 2449
BtsIMutI	CAGTG	2 cut(s)	1236, 1386
BveI	ACCTGC	1 cut(s)	2238
Cac8I	GCNNGC	4 cut(s)	377, 822, 1236, 2103
CaiI	CAGNNNCTG	2 cut(s)	1235, 2230
CfoI	GCGC	1 cut(s)	1295
Cfr13I	GGNCC	3 cut(s)	1088, 1154, 2215
ClaI	ATCGAT	1 cut(s)	1836
CseI	GACGC	2 cut(s)	344, 1795
Csp6I	GTAC	6 cut(s)	87, 1438, 1634, 1757, 1808, 2536
CviAII	CATG	5 cut(s)	1880, 1888, 2383, 2405, 2765
CviQI	GTAC	6 cut(s)	87, 1438, 1634, 1757, 1808, 2536
DdeI	CTNAG	8 cut(s)	614, 657, 1038, 2223, 2324, 2420, 2516, 2638
DraI	TTTAAA	1 cut(s)	667
EaeI	YGGCCR	4 cut(s)	1537, 1582, 2454, 2767
Eam1104I	CTCTTC	6 cut(s)	209, 428, 1433, 1467, 1941, 2433
EarI	CTCTTC	6 cut(s)	209, 428, 1433, 1467, 1941, 2433
Ecl136II	GAGCTC	1 cut(s)	2590
Eco105I	TACGTA	1 cut(s)	684
Eco130I	CCWWGG	3 cut(s)	1482, 1887, 2688
Eco24I	GRGCYC	2 cut(s)	1804, 2592
Eco31I	GGTCTC	1 cut(s)	444
Eco32I	GATATC	3 cut(s)	523, 1549, 2053
Eco47I	GGWCC	2 cut(s)	1154, 2215
Eco53kI	GAGCTC	1 cut(s)	2590
Eco57I	CTGAAG	3 cut(s)	1697, 2255, 2418
Eco81I	CCTNAGG	1 cut(s)	2420
EcoICRI	GAGCTC	1 cut(s)	2590
EcoO109I	RGGNCCY	2 cut(s)	1088, 2215
EcoRI	GAATTC	1 cut(s)	785
EcoRII	CCWGG	5 cut(s)	502, 1222, 1300, 1651, 1860
EcoRV	GATATC	3 cut(s)	523, 1549, 2053
EcoT14I	CCWWGG	3 cut(s)	1482, 1887, 2688
EcoT38I	GRGCYC	2 cut(s)	1804, 2592
ErhI	CCWWGG	3 cut(s)	1482, 1887, 2688
Esp3I	CGTCTC	1 cut(s)	637
FaeI	CATG	5 cut(s)	1883, 1891, 2386, 2408, 2768
FalI	AAGNNNNNCTT	6 cut(s)	863, 895, 2228, 2260, 2588, 2620
FatI	CATG	5 cut(s)	1879, 1887, 2382, 2404, 2764
FauI	CCCGC	1 cut(s)	2189
FbaI	TGATCA	5 cut(s)	250, 535, 952, 2778, 2799
FblI	GTMKAC	1 cut(s)	1710
Fnu4HI	GCNGC	8 cut(s)	129, 330, 1043, 1046, 1752, 1962, 1965, 2570
FokI	GGATG	8 cut(s)	188, 254, 575, 839, 876, 2084, 2425, 2456
FriOI	GRGCYC	2 cut(s)	1804, 2592
Fsp4HI	GCNGC	8 cut(s)	129, 330, 1043, 1046, 1752, 1962, 1965, 2570
FspBI	CTAG	6 cut(s)	1017, 1643, 2102, 2193, 2288, 2409
GlaI	GCGC	1 cut(s)	1294
GluI	GCNGC	8 cut(s)	129, 330, 1043, 1046, 1752, 1962, 1965, 2570
HaeIII	GGCC	6 cut(s)	1090, 1539, 1584, 2456, 2687, 2769
HapII	CCGG	1 cut(s)	2210
HgaI	GACGC	2 cut(s)	344, 1795
HhaI	GCGC	1 cut(s)	1295
Hin1II	CATG	5 cut(s)	1883, 1891, 2386, 2408, 2768
Hin6I	GCGC	1 cut(s)	1293
HinP1I	GCGC	1 cut(s)	1293
HincII	GTYRAC	3 cut(s)	2025, 2080, 2202
HindII	GTYRAC	3 cut(s)	2025, 2080, 2202
HindIII	AAGCTT	2 cut(s)	377, 1202
HpaII	CCGG	1 cut(s)	2210
HphI	GGTGA	6 cut(s)	27, 36, 485, 1658, 1882, 2294
Hpy166II	GTNNAC	9 cut(s)	799, 1161, 1711, 1759, 1785, 2025, 2080, 2202, 2285
Hpy8I	GTNNAC	9 cut(s)	799, 1161, 1711, 1759, 1785, 2025, 2080, 2202, 2285
Hpy99I	CGWCG	2 cut(s)	287, 327
HpyAV	CCTTC	8 cut(s)	57, 185, 438, 1787, 1909, 1977, 2228, 2728
HpyCH4III	ACNGT	9 cut(s)	323, 451, 648, 1158, 1165, 1231, 1390, 2311, 2535
HpyCH4IV	ACGT	3 cut(s)	683, 795, 1504
HpyCH4V	TGCA	9 cut(s)	762, 812, 889, 1045, 1964, 2009, 2416, 2677, 2705
HpyF10VI	GCNNNNNNNGC	2 cut(s)	335, 2422
HpyF3I	CTNAG	8 cut(s)	614, 657, 1038, 2223, 2324, 2420, 2516, 2638
HpySE526I	ACGT	3 cut(s)	683, 795, 1504
Hsp92II	CATG	5 cut(s)	1883, 1891, 2386, 2408, 2768
HspAI	GCGC	1 cut(s)	1293
Kpn2I	TCCGGA	1 cut(s)	2209
Ksp22I	TGATCA	5 cut(s)	250, 535, 952, 2778, 2799
LguI	GCTCTTC	1 cut(s)	1467
LmnI	GCTCC	4 cut(s)	835, 1076, 1528, 1727
Lsp1109I	GCAGC	8 cut(s)	140, 316, 1029, 1057, 1763, 1948, 1976, 2556
LweI	GCATC	5 cut(s)	337, 817, 898, 2281, 2403
MaeI	CTAG	6 cut(s)	1017, 1643, 2102, 2193, 2288, 2409
MaeII	ACGT	3 cut(s)	683, 795, 1504
MfeI	CAATTG	1 cut(s)	1518
MflI	RGATCY	6 cut(s)	199, 1486, 1765, 2029, 2189, 2707
MhlI	GDGCHC	2 cut(s)	1804, 2592
MlsI	TGGCCA	3 cut(s)	1539, 1584, 2456
MluNI	TGGCCA	3 cut(s)	1539, 1584, 2456
MlyI	GAGTC	5 cut(s)	269, 1228, 2387, 2468, 2472
MmeI	TCCRAC	1 cut(s)	2739
Mox20I	TGGCCA	3 cut(s)	1539, 1584, 2456
MroI	TCCGGA	1 cut(s)	2209
MroXI	GAANNNNTTC	1 cut(s)	723
MscI	TGGCCA	3 cut(s)	1539, 1584, 2456
MslI	CAYNNNNRTG	1 cut(s)	1391
Msp20I	TGGCCA	3 cut(s)	1539, 1584, 2456
MspA1I	CMGCKG	3 cut(s)	56, 131, 1042
MspI	CCGG	1 cut(s)	2210
MspR9I	CCNGG	5 cut(s)	504, 1224, 1302, 1653, 1862
MunI	CAATTG	1 cut(s)	1518
Mva1269I	GAATGC	2 cut(s)	1145, 2181
MvaI	CCWGG	5 cut(s)	504, 1224, 1302, 1653, 1862
MwoI	GCNNNNNNNGC	2 cut(s)	335, 2422
NcoI	CCATGG	1 cut(s)	1887
NheI	GCTAGC	1 cut(s)	2101
NlaIII	CATG	5 cut(s)	1883, 1891, 2386, 2408, 2768
NlaIV	GGNNCC	4 cut(s)	453, 1089, 1488, 2372
NmeAIII	GCCGAG	1 cut(s)	2795
NmuCI	GTSAC	4 cut(s)	510, 1012, 2133, 2431
NspI	RCATGY	1 cut(s)	2408
NspV	TTCGAA	1 cut(s)	431
OliI	CACNNNNGTG	1 cut(s)	1391
PacI	TTAATTAA	1 cut(s)	735
PciSI	GCTCTTC	1 cut(s)	1467
PcsI	WCGNNNNNNNCGW	2 cut(s)	358, 455
PctI	GAATGC	2 cut(s)	1145, 2181
PdmI	GAANNNNTTC	1 cut(s)	723
PflMI	CCANNNNNTGG	1 cut(s)	1979
PfoI	TCCNGGA	2 cut(s)	1222, 1651
PkrI	GCNGC	8 cut(s)	130, 331, 1044, 1047, 1753, 1963, 1966, 2571
PleI	GAGTC	5 cut(s)	268, 1227, 2386, 2468, 2471
PpsI	GAGTC	5 cut(s)	268, 1227, 2386, 2468, 2471
Ppu21I	YACGTR	1 cut(s)	684
PpuMI	RGGWCCY	1 cut(s)	2215
PshBI	ATTAAT	1 cut(s)	1629
Psp124BI	GAGCTC	1 cut(s)	2592
Psp5II	RGGWCCY	1 cut(s)	2215
Psp6I	CCWGG	5 cut(s)	502, 1222, 1300, 1651, 1860
PspGI	CCWGG	5 cut(s)	502, 1222, 1300, 1651, 1860
PspN4I	GGNNCC	4 cut(s)	453, 1089, 1488, 2372
PspPI	GGNCC	3 cut(s)	1088, 1154, 2215
PspPPI	RGGWCCY	1 cut(s)	2215
PstI	CTGCAG	3 cut(s)	1047, 1966, 2679
PstNI	CAGNNNCTG	2 cut(s)	1235, 2230
PsuI	RGATCY	6 cut(s)	199, 1486, 1765, 2029, 2189, 2707
PvuII	CAGCTG	2 cut(s)	131, 1042
RsaI	GTAC	6 cut(s)	88, 1439, 1635, 1758, 1809, 2537
RsaNI	GTAC	6 cut(s)	87, 1438, 1634, 1757, 1808, 2536
RseI	CAYNNNNRTG	1 cut(s)	1391
SacI	GAGCTC	1 cut(s)	2592
SapI	GCTCTTC	1 cut(s)	1467
SatI	GCNGC	8 cut(s)	129, 330, 1043, 1046, 1752, 1962, 1965, 2570
Sau96I	GGNCC	3 cut(s)	1088, 1154, 2215
SchI	GAGTC	5 cut(s)	269, 1228, 2387, 2468, 2472
ScrFI	CCNGG	5 cut(s)	504, 1224, 1302, 1653, 1862
SduI	GDGCHC	2 cut(s)	1804, 2592
SfaNI	GCATC	5 cut(s)	337, 817, 898, 2281, 2403
SfcI	CTRYAG	6 cut(s)	132, 421, 1043, 1962, 2358, 2675
SfuI	TTCGAA	1 cut(s)	431
SinI	GGWCC	2 cut(s)	1154, 2215
SmiMI	CAYNNNNRTG	1 cut(s)	1391
SmlI	CTYRAG	1 cut(s)	2375
SmoI	CTYRAG	1 cut(s)	2375
SnaBI	TACGTA	1 cut(s)	684
SsiI	CCGC	6 cut(s)	5, 54, 170, 824, 1749, 2196
SspI	AATATT	2 cut(s)	808, 907
SspMI	CTAG	6 cut(s)	1017, 1643, 2102, 2193, 2288, 2409
SstI	GAGCTC	1 cut(s)	2592
StyD4I	CCNGG	5 cut(s)	502, 1222, 1300, 1651, 1860
StyI	CCWWGG	3 cut(s)	1482, 1887, 2688
TaaI	ACNGT	9 cut(s)	323, 451, 648, 1158, 1165, 1231, 1390, 2311, 2535
TaiI	ACGT	3 cut(s)	686, 798, 1507
TaqII	GACCGA	1 cut(s)	1833
TatI	WGTACW	4 cut(s)	86, 1633, 1756, 1807
TscAI	CASTG	2 cut(s)	1236, 1393
TseFI	GTSAC	4 cut(s)	510, 1012, 2133, 2431
TseI	GCWGC	8 cut(s)	128, 329, 1042, 1045, 1751, 1961, 1964, 2569
Tsp45I	GTSAC	4 cut(s)	510, 1012, 2133, 2431
TspGWI	ACGGA	4 cut(s)	271, 986, 1515, 2358
TspRI	CASTG	2 cut(s)	1236, 1393
Van91I	CCANNNNNTGG	1 cut(s)	1979
VpaK11BI	GGWCC	2 cut(s)	1154, 2215
VspI	ATTAAT	1 cut(s)	1629
XapI	RAATTY	6 cut(s)	13, 106, 785, 1824, 2108, 2495
XceI	RCATGY	1 cut(s)	2408
XmiI	GTMKAC	1 cut(s)	1710
XmnI	GAANNNNTTC	1 cut(s)	723
XspI	CTAG	6 cut(s)	1017, 1643, 2102, 2193, 2288, 2409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.