Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0014494

Physical Location & Seq

Genomic Coordinates Reverse (-)

56404077 .. 56409468

Gene Structure 5392 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG303800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2817 bp

ATGGCAGACAACTCCGGGAATCCGCCGTCGACGCCGGACTCACCAACGTCGGTAGGGTTCAACACCGACCAGCTGCCGCACACGAGCCGCACCTCCGAGAACTTCTCCTCCTCCGGCGACGAGGCCGACGTCGACCCCAATATCCTCCAAGATGACGTGGAGGACGACCTCCTTGTCGAAGAAGAGCCGGAGGGGGAGGATCTCCTGGAAGGAAATTACATGGAGGACTACCGGAGAATGGATGAGATGGATCAGTACGAGTCGGTGGGGCTGGATGATTCGGCTGAGGACGAGAGGGATTTGGATCAGATCATGGCGGATCGCCGGGCGGCTGAACTTGAGCTTGATAAACGCGACGGCGTACCGGCCTCTCGCAAGCTTCCTCATCTTCTTCATGACCAAGATACTGATGAGGACAACTACAGGCCTTCAAAGAGGGCGAGAGCTGACTTCAGGCCTCCGAGGAGCGCAGATGATACCGATGGAATGCCGAGCTCGCCTGGAAGATCACAGCCTGGACACTCCAGAGAGGATGTTCCCATGACTGATCAGACTGATGATCAATATGAGGATGATGAAGACGAAGATGGGTTTGACATGTATCGTGTCCAGGGAACACTCCGAGAGTGGGTTACTAGAGATGAAGTACGAAGATTTATTGCCAAGAAATTTAAAGAGTTTCTACTCACCTATACAAATCAAAGGAACGATCATGGTGACATTGAATATGTTCGCCTTATTAATGAGATGGTGTTAGCGAACAAGTGTAGTCTGGAGATTGACTACAAGCAATTCATCTATGTTCATCCCAATATTGCTATTTGGTTGGCTGATGCGCCCCAGTCTGTCCTGGAAGTCATGGAAGATGTTGCGAAGAATGTTGTGTTTAATTTACACCCCAATTACAAACTTATCCATCAAAAGATCTATGTTCGCATAACTAACTTACCTGTTTATGATCAGATTCGTAACATTAGGCAGATTCATTTGAATACCATGATTCGTATTGGAGGAGTTGTAACCCGACGCTCTGGGGTGTTTCCCCAGTTGCAACAAGTAAAGTATGATTGCAATAAATGTGGAGCAGTCTTGGGGCCATTCTTTCAGAATTCTTATTCGGAAGTTAAAGTTGGTTCTTGCCCTGAGTGTCAATCCAAAGGGCCATTTACTGTCAATATTGAGCAGACAATATACAGGAATTATCAAAAGCTTACGCTCCAGGAGAGCCCAGGAATAGTGCCTGCAGGTCGGCTTCCAAGATACAAGGAAGTGATACTATTGAATGATCTAATTGATTGTGCTCGTCCAGGGGAAGAGATCGAGGTCACTGGTATTTATTCAAACAATTTTGACTTGTCTTTGAATACAAAGAATGGTTTTCCTGTATTTGCTACTGTGGTTGAAGCAAATTATATCACGAAGAAGCAAGACCTCTTTTCTGCTTACAAACTTACTGAGGAAGACAAACAACAGATCGAAGCGTTATCTAAAGACCCAAGAATCGGAGAAAAGCTTGTCAAGTCCATTGCTCCATCTATATATGGCCATGAGGACATAAAGACTGCAATAGCTCTTGCCATGTTTGGAGGCCAAGAGAAAAATGTTGAGGGAAAACACCGACTGCGAGGTGATATAAACGTACTTCTCCTAGGTGATCCAGGCACAGCAAAATCACAATTTCTTAAGTATGTTGAAAAGACTGGGCAGAGGGCTGTGTATACCACTGGTAAAGGAGCATCTGCTGTGGGGCTTACAGCAGCTGTGCACAAGGATCCAGTTACAAAGGAGTGGACCCTTGAAGGAGGAGCCCTTGTTCTAGCAGACAAGGGAATATGCCTTATTGATGAATTTGACAAAATGAATGATCAGGACAGGGTGAGTATTCATGAAGCAATGGAGCAGCAAAGTATTAGCATATCGAAAGCTGGGATTGTCACTTCTCTCCAAGCACGCTGCTCTGTCATTGCAGCTGCAAACCCTGTTGGAGGAAGATATGATTCCTCAAAAACATTTACACAAAATGTTGAGTTGACAGATCCCATCATCTCTCGTTTTGACATCCTTTGTGTTGTGAAGGATGTGGTTGACCCCGTCACTGATGAAATGCTTGCAAAGTTTGTAGTTGATAGTCATTTCAAGTCGCAACCCAAGGGGGCCAATATGGATGATATAACCTTAGACGAGTCTCAAGAAGATATCCAAGCCTCCACCAGTCCAGTAGATCCTGATATACTTCCTCAAGACTTGCTAAAGAAATACTTGACTTTTGCCAAGTTATATGTATTCCCAAGGTTGCACGATGCTGATTTGGATAAACTCTCAAATGTTTATGCTGAACTGCGGCGAGAATCTTCGCATGGACAAGGAGTACCAATAGCCGTAAGGCACATAGAATCAATGATACGAATGTCTGAAGCACATGCCAGAATGCACCTTAGACATCAAGTAACCGGAGATGATGTGGACATGGCAATTCGCGTAGCACTCGACTCATTCATCTCAACCCAAAAATTTGGAGTGCAGAAAGCTCTACAAAAGAGTTTCAAAAGGCATATCACTTTCAAGAAAGATAGGAATGAACTGCTCCTCTTTCTTCTCAAAGAGCTTGTGAAGAATGCATTGCAGTTTGAAGAAATTGTTTCTGGCTCTTCTTTAGGGCTTACCCATATTGATGTAAAAGTGGGAGATTTGCTGAACAAGGCAAACGAGCATGAAATCTATGATCTAAACCCCTTCTTTACCAGTGCTCTTTTTACACGTGCCAATTTTGTGTTGGATGAAGAGCGGGGATTGATAAGGCATCGCCTTTCAGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM2_N MCM_N MCM_OB

Protein Analysis

938

Amino Acids

105.83

Weight (kDa)

5.01

Isoelectric Point (pI)

47.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM2_N	PF12619	48 - 156	8.7e-12	Mini-chromosome maintenance protein 2
MCM_N	PF14551	222 - 315	1.5e-17	MCM N-terminal domain
MCM_OB	PF17207	321 - 448	2.2e-34	MCM OB domain
MCM	PF00493	489 - 711	4.9e-104	MCM P-loop domain
AAA_5	PF07728	546 - 664	7.4e-07	AAA domain (dynein-related subfamily)
Mg_chelatase	PF01078	600 - 691	1.1e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	748 - 831	8.9e-25	MCM AAA-lid domain
WHD_MCM2	PF23669	856 - 932	6.3e-25	DNA replication licensing factor MCM2-like, winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0014494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44900 AT1G44900
fragaria_vesca	FvH4_4g22180
malus_domestica	MD13G1134100.v1.1 MD16G1144700.v1.1
prunus_persica	Prupe.1G211500_v2.0.a1
pyrus_communis	pycom13g11680 pycom16g11400
rosa_chinensis	RchiOBHm_Chr4g0428411
rosa_laevigata	RLG00000007153
rosa_multiflora	Rmu_sc0004698.1_g000003
rosa_roxburghii	Rroxscaffold_5G00370080
rosa_rugosa	Rorug04G0225200
rosa_samantha	Rh4BG288100 Rh4CG303800 Rh4DG285200
rosa_wichuraiana	Rw4G024520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	173
AatII	GACGTC	1 cut(s)	132
Acc36I	ACCTGC	1 cut(s)	1235
AccBSI	CCGCTC	1 cut(s)	2785
AccI	GTMKAC	3 cut(s)	29, 132, 1718
AccII	CGCG	2 cut(s)	354, 2478
AciI	CCGC	7 cut(s)	23, 77, 88, 317, 329, 2341, 2785
AclWI	GGATC	9 cut(s)	207, 258, 312, 327, 1649, 1766, 1779, 2030, 2216
AcoI	YGGCCR	1 cut(s)	1543
AcsI	RAATTY	4 cut(s)	668, 1108, 1847, 2510
AcuI	CTGAAG	2 cut(s)	436, 2433
AcvI	CACGTG	1 cut(s)	2759
AcyI	GRCGYC	2 cut(s)	32, 129
AfaI	GTAC	5 cut(s)	257, 363, 648, 1641, 2370
AfiI	CCNNNNNNNGG	4 cut(s)	238, 628, 1502, 1985
AflII	CTTAAG	1 cut(s)	1682
AflIII	ACRYGT	2 cut(s)	597, 2756
AjiI	CACGTC	1 cut(s)	157
AjnI	CCWGG	9 cut(s)	204, 499, 514, 609, 849, 1218, 1228, 1306, 1657
AjuI	GAANNNNNNNTTGG	2 cut(s)	1113, 1145
AloI	GAACNNNNNNTCC	4 cut(s)	92, 124, 1797, 1829
Alw21I	GWGCWC	4 cut(s)	497, 1303, 1767, 2749
Alw26I	GTCTC	1 cut(s)	2190
Alw44I	GTGCAC	1 cut(s)	1763
AlwI	GGATC	9 cut(s)	207, 258, 312, 327, 1649, 1766, 1779, 2030, 2216
AlwNI	CAGNNNCTG	1 cut(s)	1760
AoxI	GGCC	9 cut(s)	123, 366, 425, 455, 1094, 1160, 1543, 1588, 2154
ApaLI	GTGCAC	1 cut(s)	1763
ApeKI	GCWGC	6 cut(s)	73, 1757, 1900, 1953, 1967, 1970
ApoI	RAATTY	4 cut(s)	668, 1108, 1847, 2510
AseI	ATTAAT	1 cut(s)	741
Asp700I	GAANNNNTTC	1 cut(s)	729
AspA2I	CCTAGG	1 cut(s)	1648
AspLEI	GCGC	2 cut(s)	470, 838
AspS9I	GGNCC	4 cut(s)	1094, 1160, 1791, 2154
AsuC2I	CCSGG	2 cut(s)	16, 326
AsuHPI	GGTGA	6 cut(s)	33, 679, 728, 1640, 1664, 1888
AvaII	GGWCC	1 cut(s)	1791
AvrII	CCTAGG	1 cut(s)	1648
BaeGI	GKGCMC	1 cut(s)	1767
BaeI	ACNNNNGTAYC	2 cut(s)	2393, 2426
BalI	TGGCCA	1 cut(s)	1545
BamHI	GGATCC	1 cut(s)	1771
BanII	GRGCYC	3 cut(s)	497, 1229, 1810
BauI	CACGAG	1 cut(s)	82
BbrPI	CACGTG	1 cut(s)	2759
BbsI	GAAGAC	2 cut(s)	585, 1467
Bbv12I	GWGCWC	4 cut(s)	497, 1303, 1767, 2749
BbvCI	CCTCAGC	1 cut(s)	285
BbvI	GCAGC	6 cut(s)	60, 1769, 1912, 1940, 1957, 1979
BccI	CCATC	7 cut(s)	241, 476, 581, 742, 924, 1540, 2048
BceAI	ACGGC	3 cut(s)	10, 373, 2363
BciT130I	CCWGG	9 cut(s)	206, 501, 516, 611, 851, 1220, 1230, 1308, 1659
BclI	TGATCA	4 cut(s)	547, 559, 958, 1864
BcnI	CCSGG	2 cut(s)	16, 326
BcoDI	GTCTC	1 cut(s)	2190
BfaI	CTAG	3 cut(s)	636, 1649, 1817
BfmI	CTRYAG	2 cut(s)	421, 1242
BfrI	CTTAAG	1 cut(s)	1682
BfuAI	ACCTGC	1 cut(s)	1235
BglII	AGATCT	1 cut(s)	924
BlnI	CCTAGG	1 cut(s)	1648
Bme18I	GGWCC	1 cut(s)	1791
BmgBI	CACGTC	1 cut(s)	157
BmgT120I	GGNCC	4 cut(s)	1094, 1160, 1791, 2154
BmiI	GGNNCC	5 cut(s)	1095, 1773, 1793, 1807, 2155
BmrI	ACTGGG	3 cut(s)	835, 1039, 1710
BmsI	GCATC	4 cut(s)	823, 1745, 2290, 2809
BmuI	ACTGGG	3 cut(s)	835, 1039, 1710
BpiI	GAAGAC	2 cut(s)	585, 1467
BplI	GAGNNNNNCTC	2 cut(s)	89, 121
BpmI	CTGGAG	3 cut(s)	508, 794, 1202
Bpu10I	CCTNAGC	1 cut(s)	285
BpuEI	CTTGAG	3 cut(s)	359, 2172, 2223
BpuMI	CCSGG	2 cut(s)	16, 326
BsaAI	YACGTR	1 cut(s)	2759
BsaBI	GATNNNNATC	1 cut(s)	303
BsaHI	GRCGYC	2 cut(s)	32, 129
BsaJI	CCNNGG	7 cut(s)	461, 610, 1228, 1307, 1648, 2148, 2288
BsaWI	WCCGGW	2 cut(s)	231, 2450
BsaXI	ACNNNNNCTCC	8 cut(s)	92, 122, 1002, 1032, 1797, 1827, 2445, 2475
Bsc4I	CCNNNNNNNGG	4 cut(s)	238, 628, 1502, 1985
Bse118I	RCCGGY	1 cut(s)	364
Bse1I	ACTGG	9 cut(s)	841, 1045, 1333, 1705, 1729, 1775, 2211, 2216, 2742
Bse3DI	GCAATG	4 cut(s)	1524, 1899, 1962, 2618
Bse8I	GATNNNNATC	1 cut(s)	303
BseBI	CCWGG	9 cut(s)	206, 501, 516, 611, 851, 1220, 1230, 1308, 1659
BseDI	CCNNGG	7 cut(s)	461, 610, 1228, 1307, 1648, 2148, 2288
BseGI	GGATG	9 cut(s)	247, 280, 538, 577, 805, 2058, 2083, 2170, 2782
BseJI	GATNNNNATC	1 cut(s)	303
BseLI	CCNNNNNNNGG	4 cut(s)	238, 628, 1502, 1985
BseMI	GCAATG	4 cut(s)	1524, 1899, 1962, 2618
BseMII	CTCAG	3 cut(s)	276, 1134, 1446
BseNI	ACTGG	9 cut(s)	841, 1045, 1333, 1705, 1729, 1775, 2211, 2216, 2742
BseRI	GAGGAG	6 cut(s)	97, 100, 478, 1026, 1818, 2576
BseSI	GKGCMC	1 cut(s)	1767
BseXI	GCAGC	6 cut(s)	60, 1769, 1912, 1940, 1957, 1979
BseYI	CCCAGC	1 cut(s)	1925
BsgI	GTGCAG	1 cut(s)	2540
Bsh1236I	CGCG	2 cut(s)	354, 2478
BshFI	GGCC	9 cut(s)	125, 368, 427, 457, 1096, 1162, 1545, 1590, 2156
BsiHKAI	GWGCWC	4 cut(s)	497, 1303, 1767, 2749
BsiSI	CCGG	8 cut(s)	15, 35, 114, 188, 232, 325, 365, 2451
BslI	CCNNNNNNNGG	4 cut(s)	238, 628, 1502, 1985
BsmAI	GTCTC	1 cut(s)	2190
BsmI	GAATGC	3 cut(s)	492, 2433, 2620
BsnI	GGCC	9 cut(s)	125, 368, 427, 457, 1096, 1162, 1545, 1590, 2156
Bsp1286I	GDGCHC	6 cut(s)	497, 1229, 1303, 1767, 1810, 2749
BspACI	CCGC	7 cut(s)	23, 77, 88, 317, 329, 2341, 2785
BspANI	GGCC	9 cut(s)	125, 368, 427, 457, 1096, 1162, 1545, 1590, 2156
BspCNI	CTCAG	3 cut(s)	277, 1135, 1447
BspFNI	CGCG	2 cut(s)	354, 2478
BspHI	TCATGA	2 cut(s)	394, 1885
BspLI	GGNNCC	5 cut(s)	1095, 1773, 1793, 1807, 2155
BspMAI	CTGCAG	1 cut(s)	1246
BspMI	ACCTGC	1 cut(s)	1235
BspPI	GGATC	9 cut(s)	207, 258, 312, 327, 1649, 1766, 1779, 2030, 2216
BspQI	GCTCTTC	3 cut(s)	177, 2653, 2775
BspTI	CTTAAG	1 cut(s)	1682
BsrBI	CCGCTC	1 cut(s)	2785
BsrDI	GCAATG	4 cut(s)	1524, 1899, 1962, 2618
BsrFI	RCCGGY	1 cut(s)	364
BsrI	ACTGG	9 cut(s)	841, 1045, 1333, 1705, 1729, 1775, 2211, 2216, 2742
BssAI	RCCGGY	1 cut(s)	364
BssECI	CCNNGG	7 cut(s)	461, 610, 1228, 1307, 1648, 2148, 2288
BssNAI	GTATAC	1 cut(s)	1719
BssNI	GRCGYC	2 cut(s)	32, 129
BssSI	CACGAG	1 cut(s)	82
BssT1I	CCWWGG	3 cut(s)	1648, 2148, 2288
Bst1107I	GTATAC	1 cut(s)	1719
Bst2BI	CACGAG	1 cut(s)	82
Bst2UI	CCWGG	9 cut(s)	206, 501, 516, 611, 851, 1220, 1230, 1308, 1659
Bst4CI	ACNGT	2 cut(s)	1171, 1396
Bst6I	CTCTTC	4 cut(s)	177, 1308, 2653, 2775
BstACI	GRCGYC	2 cut(s)	32, 129
BstAFI	CTTAAG	1 cut(s)	1682
BstBAI	YACGTR	1 cut(s)	2759
BstC8I	GCNNGC	5 cut(s)	377, 497, 1242, 1951, 2109
BstDEI	CTNAG	5 cut(s)	285, 1143, 1455, 2176, 2435
BstENI	CCTNNNNNAGG	1 cut(s)	1983
BstF5I	GGATG	9 cut(s)	247, 280, 538, 577, 805, 2058, 2083, 2170, 2782
BstFNI	CGCG	2 cut(s)	354, 2478
BstHHI	GCGC	2 cut(s)	470, 838
BstMAI	GTCTC	1 cut(s)	2190
BstMWI	GCNNNNNNNGC	2 cut(s)	31, 496
BstNI	CCWGG	9 cut(s)	206, 501, 516, 611, 851, 1220, 1230, 1308, 1659
BstNSI	RCATGY	2 cut(s)	601, 2423
BstSFI	CTRYAG	2 cut(s)	421, 1242
BstSLI	GKGCMC	1 cut(s)	1767
BstUI	CGCG	2 cut(s)	354, 2478
BstV1I	GCAGC	6 cut(s)	60, 1769, 1912, 1940, 1957, 1979
BstV2I	GAAGAC	2 cut(s)	585, 1467
BstX2I	RGATCY	5 cut(s)	199, 924, 1771, 2035, 2221
BstXI	CCANNNNNNTGG	1 cut(s)	2513
BstYI	RGATCY	5 cut(s)	199, 924, 1771, 2035, 2221
BstZ17I	GTATAC	1 cut(s)	1719
BsuRI	GGCC	9 cut(s)	125, 368, 427, 457, 1096, 1162, 1545, 1590, 2156
BtgZI	GCGATG	1 cut(s)	2786
BtrI	CACGTC	1 cut(s)	157
BtsCI	GGATG	9 cut(s)	247, 280, 538, 577, 805, 2058, 2083, 2170, 2782
BtsIMutI	CAGTG	4 cut(s)	1326, 1722, 2094, 2749
BveI	ACCTGC	1 cut(s)	1235
Cac8I	GCNNGC	5 cut(s)	377, 497, 1242, 1951, 2109
CaiI	CAGNNNCTG	1 cut(s)	1760
CciI	TCATGA	2 cut(s)	394, 1885
CfoI	GCGC	2 cut(s)	470, 838
Cfr10I	RCCGGY	1 cut(s)	364
Cfr13I	GGNCC	4 cut(s)	1094, 1160, 1791, 2154
CseI	GACGC	2 cut(s)	40, 1035
Csp6I	GTAC	5 cut(s)	256, 362, 647, 1640, 2369
CspCI	CAANNNNNGTGG	2 cut(s)	1060, 1095
CviQI	GTAC	5 cut(s)	256, 362, 647, 1640, 2369
DdeI	CTNAG	5 cut(s)	285, 1143, 1455, 2176, 2435
DraI	TTTAAA	1 cut(s)	673
DrdI	GACNNNNNNGTC	1 cut(s)	173
DseDI	GACNNNNNNGTC	1 cut(s)	173
EaeI	YGGCCR	1 cut(s)	1543
Eam1104I	CTCTTC	4 cut(s)	177, 1308, 2653, 2775
EarI	CTCTTC	4 cut(s)	177, 1308, 2653, 2775
EciI	GGCGGA	2 cut(s)	12, 332
Ecl136II	GAGCTC	1 cut(s)	495
Eco130I	CCWWGG	3 cut(s)	1648, 2148, 2288
Eco147I	AGGCCT	2 cut(s)	427, 457
Eco24I	GRGCYC	3 cut(s)	497, 1229, 1810
Eco32I	GATATC	1 cut(s)	2197
Eco47I	GGWCC	1 cut(s)	1791
Eco53kI	GAGCTC	1 cut(s)	495
Eco57I	CTGAAG	2 cut(s)	436, 2433
Eco72I	CACGTG	1 cut(s)	2759
EcoICRI	GAGCTC	1 cut(s)	495
EcoNI	CCTNNNNNAGG	1 cut(s)	1983
EcoRI	GAATTC	1 cut(s)	1108
EcoRII	CCWGG	9 cut(s)	204, 499, 514, 609, 849, 1218, 1228, 1306, 1657
EcoRV	GATATC	1 cut(s)	2197
EcoT14I	CCWWGG	3 cut(s)	1648, 2148, 2288
EcoT22I	ATGCAT	1 cut(s)	2620
EcoT38I	GRGCYC	3 cut(s)	497, 1229, 1810
ErhI	CCWWGG	3 cut(s)	1648, 2148, 2288
FalI	AAGNNNNNCTT	2 cut(s)	2243, 2275
FauI	CCCGC	1 cut(s)	2778
FbaI	TGATCA	4 cut(s)	547, 559, 958, 1864
FblI	GTMKAC	3 cut(s)	29, 132, 1718
FokI	GGATG	9 cut(s)	254, 287, 545, 584, 792, 2045, 2090, 2177, 2789
FriOI	GRGCYC	3 cut(s)	497, 1229, 1810
FspBI	CTAG	3 cut(s)	636, 1649, 1817
GlaI	GCGC	2 cut(s)	469, 837
GsaI	CCCAGC	1 cut(s)	1929
GsuI	CTGGAG	3 cut(s)	508, 794, 1202
HaeIII	GGCC	9 cut(s)	125, 368, 427, 457, 1096, 1162, 1545, 1590, 2156
HapII	CCGG	8 cut(s)	15, 35, 114, 188, 232, 325, 365, 2451
HgaI	GACGC	2 cut(s)	40, 1035
HhaI	GCGC	2 cut(s)	470, 838
Hin1I	GRCGYC	2 cut(s)	32, 129
Hin6I	GCGC	2 cut(s)	468, 836
HinP1I	GCGC	2 cut(s)	468, 836
HincII	GTYRAC	4 cut(s)	30, 133, 2031, 2086
HindII	GTYRAC	4 cut(s)	30, 133, 2031, 2086
HindIII	AAGCTT	3 cut(s)	377, 1208, 1511
HpaII	CCGG	8 cut(s)	15, 35, 114, 188, 232, 325, 365, 2451
HphI	GGTGA	6 cut(s)	33, 679, 728, 1640, 1664, 1888
Hpy166II	GTNNAC	8 cut(s)	30, 133, 1719, 1765, 1791, 2031, 2086, 2464
Hpy8I	GTNNAC	8 cut(s)	30, 133, 1719, 1765, 1791, 2031, 2086, 2464
Hpy99I	CGWCG	8 cut(s)	31, 34, 52, 122, 131, 134, 359, 1029
HpyAV	CCTTC	5 cut(s)	203, 438, 1793, 2068, 2743
HpyCH4III	ACNGT	2 cut(s)	1171, 1396
HpyCH4IV	ACGT	5 cut(s)	47, 129, 156, 1638, 2758
HpyF10VI	GCNNNNNNNGC	2 cut(s)	31, 496
HpyF3I	CTNAG	5 cut(s)	285, 1143, 1455, 2176, 2435
HpySE526I	ACGT	5 cut(s)	47, 129, 156, 1638, 2758
Hsp92I	GRCGYC	2 cut(s)	32, 129
HspAI	GCGC	2 cut(s)	468, 836
Ksp22I	TGATCA	4 cut(s)	547, 559, 958, 1864
LguI	GCTCTTC	3 cut(s)	177, 2653, 2775
LmnI	GCTCC	8 cut(s)	465, 1082, 1221, 1534, 1733, 1805, 1897, 2589
Lsp1109I	GCAGC	6 cut(s)	60, 1769, 1912, 1940, 1957, 1979
LweI	GCATC	4 cut(s)	823, 1745, 2290, 2809
MaeI	CTAG	3 cut(s)	636, 1649, 1817
MaeII	ACGT	5 cut(s)	47, 129, 156, 1638, 2758
MaeIII	GTNAC	9 cut(s)	631, 716, 968, 1018, 1324, 1777, 1933, 2092, 2446
MbiI	CCGCTC	1 cut(s)	2785
MflI	RGATCY	5 cut(s)	199, 924, 1771, 2035, 2221
MhlI	GDGCHC	6 cut(s)	497, 1229, 1303, 1767, 1810, 2749
MlsI	TGGCCA	1 cut(s)	1545
MluNI	TGGCCA	1 cut(s)	1545
MlyI	GAGTC	4 cut(s)	32, 269, 2192, 2483
MmeI	TCCRAC	2 cut(s)	1963, 2754
Mox20I	TGGCCA	1 cut(s)	1545
Mph1103I	ATGCAT	1 cut(s)	2620
MroXI	GAANNNNTTC	1 cut(s)	729
MscI	TGGCCA	1 cut(s)	1545
MseI	TTAA	5 cut(s)	672, 741, 888, 1125, 1683
MslI	CAYNNNNRTG	1 cut(s)	2670
Msp20I	TGGCCA	1 cut(s)	1545
MspA1I	CMGCKG	3 cut(s)	73, 1760, 1970
MspCI	CTTAAG	1 cut(s)	1682
MspI	CCGG	8 cut(s)	15, 35, 114, 188, 232, 325, 365, 2451
Mva1269I	GAATGC	3 cut(s)	492, 2433, 2620
MvaI	CCWGG	9 cut(s)	206, 501, 516, 611, 851, 1220, 1230, 1308, 1659
MvnI	CGCG	2 cut(s)	354, 2478
MwoI	GCNNNNNNNGC	2 cut(s)	31, 496
NciI	CCSGG	2 cut(s)	16, 326
NlaIV	GGNNCC	5 cut(s)	1095, 1773, 1793, 1807, 2155
NmeAIII	GCCGAG	1 cut(s)	516
NmuCI	GTSAC	4 cut(s)	716, 1324, 1933, 2092
NsiI	ATGCAT	1 cut(s)	2620
NspI	RCATGY	2 cut(s)	601, 2423
PagI	TCATGA	2 cut(s)	394, 1885
PceI	AGGCCT	2 cut(s)	427, 457
PciI	ACATGT	1 cut(s)	597
PciSI	GCTCTTC	3 cut(s)	177, 2653, 2775
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 162
PctI	GAATGC	3 cut(s)	492, 2433, 2620
PdmI	GAANNNNTTC	1 cut(s)	729
PfeI	GAWTC	9 cut(s)	19, 278, 964, 982, 1000, 1500, 1997, 2348, 2393
PflFI	GACNNNGTC	1 cut(s)	2090
PfoI	TCCNGGA	4 cut(s)	14, 204, 849, 1218
PleI	GAGTC	4 cut(s)	32, 268, 2191, 2483
PmaCI	CACGTG	1 cut(s)	2759
PmlI	CACGTG	1 cut(s)	2759
PpsI	GAGTC	4 cut(s)	32, 268, 2191, 2483
Ppu21I	YACGTR	1 cut(s)	2759
PscI	ACATGT	1 cut(s)	597
PshBI	ATTAAT	1 cut(s)	741
Psp124BI	GAGCTC	1 cut(s)	497
Psp6I	CCWGG	9 cut(s)	204, 499, 514, 609, 849, 1218, 1228, 1306, 1657
PspCI	CACGTG	1 cut(s)	2759
PspFI	CCCAGC	1 cut(s)	1925
PspGI	CCWGG	9 cut(s)	204, 499, 514, 609, 849, 1218, 1228, 1306, 1657
PspN4I	GGNNCC	5 cut(s)	1095, 1773, 1793, 1807, 2155
PspPI	GGNCC	4 cut(s)	1094, 1160, 1791, 2154
PstI	CTGCAG	1 cut(s)	1246
PstNI	CAGNNNCTG	1 cut(s)	1760
PsuI	RGATCY	5 cut(s)	199, 924, 1771, 2035, 2221
PsyI	GACNNNGTC	1 cut(s)	2090
PvuII	CAGCTG	3 cut(s)	73, 1760, 1970
RsaI	GTAC	5 cut(s)	257, 363, 648, 1641, 2370
RsaNI	GTAC	5 cut(s)	256, 362, 647, 1640, 2369
RseI	CAYNNNNRTG	1 cut(s)	2670
SacI	GAGCTC	1 cut(s)	497
SalI	GTCGAC	2 cut(s)	28, 131
SapI	GCTCTTC	3 cut(s)	177, 2653, 2775
SaqAI	TTAA	5 cut(s)	672, 741, 888, 1125, 1683
Sau96I	GGNCC	4 cut(s)	1094, 1160, 1791, 2154
SbfI	CCTGCAGG	1 cut(s)	1246
SchI	GAGTC	4 cut(s)	32, 269, 2192, 2483
SdaI	CCTGCAGG	1 cut(s)	1246
SduI	GDGCHC	6 cut(s)	497, 1229, 1303, 1767, 1810, 2749
SfaNI	GCATC	4 cut(s)	823, 1745, 2290, 2809
SfcI	CTRYAG	2 cut(s)	421, 1242
SgrDI	CGTCGACG	1 cut(s)	28
SinI	GGWCC	1 cut(s)	1791
SmiMI	CAYNNNNRTG	1 cut(s)	2670
SmlI	CTYRAG	4 cut(s)	338, 1682, 2187, 2238
SmoI	CTYRAG	4 cut(s)	338, 1682, 2187, 2238
Sse8387I	CCTGCAGG	1 cut(s)	1246
SseBI	AGGCCT	2 cut(s)	427, 457
SsiI	CCGC	7 cut(s)	23, 77, 88, 317, 329, 2341, 2785
SspI	AATATT	2 cut(s)	814, 1177
SspMI	CTAG	3 cut(s)	636, 1649, 1817
SstI	GAGCTC	1 cut(s)	497
StuI	AGGCCT	2 cut(s)	427, 457
StyI	CCWWGG	3 cut(s)	1648, 2148, 2288
TaaI	ACNGT	2 cut(s)	1171, 1396
TaiI	ACGT	5 cut(s)	50, 132, 159, 1641, 2761
TaqI	TCGA	7 cut(s)	29, 132, 177, 1320, 1476, 1919, 2487
TauI	GCSGC	4 cut(s)	79, 90, 332, 2344
TfiI	GAWTC	9 cut(s)	19, 278, 964, 982, 1000, 1500, 1997, 2348, 2393
Tru1I	TTAA	5 cut(s)	672, 741, 888, 1125, 1683
Tru9I	TTAA	5 cut(s)	672, 741, 888, 1125, 1683
TscAI	CASTG	4 cut(s)	1333, 1729, 2101, 2749
TseFI	GTSAC	4 cut(s)	716, 1324, 1933, 2092
TseI	GCWGC	6 cut(s)	73, 1757, 1900, 1953, 1967, 1970
Tsp45I	GTSAC	4 cut(s)	716, 1324, 1933, 2092
TspRI	CASTG	4 cut(s)	1333, 1729, 2101, 2749
Tth111I	GACNNNGTC	1 cut(s)	2090
Vha464I	CTTAAG	1 cut(s)	1682
VneI	GTGCAC	1 cut(s)	1763
VpaK11BI	GGWCC	1 cut(s)	1791
VspI	ATTAAT	1 cut(s)	741
XagI	CCTNNNNNAGG	1 cut(s)	1983
XapI	RAATTY	4 cut(s)	668, 1108, 1847, 2510
XceI	RCATGY	2 cut(s)	601, 2423
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2770
XmaJI	CCTAGG	1 cut(s)	1648
XmiI	GTMKAC	3 cut(s)	29, 132, 1718
XmnI	GAANNNNTTC	1 cut(s)	729
XspI	CTAG	3 cut(s)	636, 1649, 1817
ZraI	GACGTC	1 cut(s)	130
Zsp2I	ATGCAT	1 cut(s)	2620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.