Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014494

Physical Location & Seq

Genomic Coordinates Forward (+)

10269053 .. 10274300

Gene Structure 5248 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1134100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGGCAGATAATCATCCGTCGACGCCGGATTCGCCGACGTCGGTCGGGTTCAACACCGACCAGCTGCCGCACACGAGCCGCACCTCCGAGAACTTCTCATCCTCCTCTTCCGACGACGAGGCCGCCGTCGACCCCGAAATCGTCCGAGATGAACCCGACGACGACCCCCTCCTTGAAGAGGAGGAGGGAGAGGATCTCTTCCACGACAATTTCTTGGATGATTATCGGAGATTGGATGGGCACGATCAATACGAGTCGGTGGGGCTGGATGATTCCGTTGAGGACGAGAGGGATTTGGATCAGATCATGGCCGACCGCCGGGCCGCGGAAGTCGAGCTTGATACCCGCGACGGCGTCCACACCTCCCGCAAGCTCCCCCATCTTCTCCATGACCAAGATACTGATGATGATAGCTACCGGCCTTCGAAGAGAGCAAGGGCAGATTTCAGGCCTCCGAGAAGCTACGATGATACAGATGGAATGCAGAGTTCACCCGGAAGATCACAGTCACAGCCTGAACACTCCAGAGAGACTGATGTTGACGGCGATGAGGATGATGAAGACGAAGACTTTAATATGTATTGTGTTCAGGGAACGCTTAGAGAGTGGGTTACGAGAGACGAAGTGCGAAGATTCATAGGCAGAAAATTCAAAGAGTTTTTACTCACCTATGTGAAAAAGGATCACAGTGATATCGAATGTGTGCGCCTCATTAATGAGATGGTGTTAGCCAATAAGTGTAGTCTGGAGATTGATTACAAGCAATTCATCCATGTTCATCCCAATATTGCCATTTGGTTGGCTGATGCCCCCCAGTCTGTCCTTGAAGTTATGGAAGATGTTGCCAAGAATGTTGTGTTTACTTTACACCCCAATTACAAACGTATCCATCAAAAGATCTATGTTCGGATAACTAACTTACCTGTTTACGATCAGATTCGCAACATTAGGCAGGTCCATTTGAATACCATGATTCGTATTGGAGGGGTTGTGACCCGACGCTCTGGGGTGTTTCCCCAGTTGCAGCAAGTAAAGTATGATTGCAATAAATGTGGGGCAATCTTGGGGCCATTCTTTCAGAATTCTTATTCTGAAGTCAAAGTTGGTTCTTGCCCTGAATGCCAATCAAAAGGACCATTCAGTGTCAATATTGAGCAGACAATATACAGGAACTATCAAAAACTTACACTCCAAGAGAGCCCTGGAATTGTCCCCGCAGGTCGGCTTCCAAGATACAAGGAAGTGATACTATTGAATGATCTAATTGATTGTGCTCGTCCAGGGGAAGAGATTGAGGTCACTGGCATTTATTCAAATAATTTTGACTTGTCTTTGAATACAAAGAACGGGTTTCCTGTATTTGCCACTGTGGTTGAAGCAAATTATATCACTAAGAAGCAAGACCTCTTTTCTGCTTACAAACTTACTCAGGAAGACAAAGAAGAGATTGAAAAGTTGTCAAAAGACCCAAGAATAGGAGAAAGGCTTATAAAGTCAATTGCGCCATCAATCTATGGCCATGAGGACATAAAGACTGCAATAGCTCTTGCTATGTTTGGAGGCCAAGAGAAAAATGTTGAGGGAAAACACAGGCTAAGAGGGGATATAAATGTACTTCTCTTAGGTGATCCTGGCACGGCAAAATCACAGTTTCTTAAGTATGTTGAGAAGACTGGGCAAAGGGCTGTGTATACTACTGGTAAAGGAGCATCTGCTGTGGGGCTCACAGCAGCTGTTCACAAGGATCCTGTTACAAGGGAGTGGACTCTTGAAGGAGGGGCGCTTGTTCTAGCTGACAAGGGAATATGTCTTATTGATGAATTTGACAAAATGAACGATCAGGATAGGGTGAGTATTCATGAAGCAATGGAGCAGCAAAGCATTAGCATATCAAAAGCTGGGATTGTCACTTCTCTCCAAGCACGCTGCTCTGTCATTGCAGCTGCAAACCCTGTTGGAGGAAGATATGATTCCTCTAAAACCTTTGCACAAAATGTTGAGTTAACAGATCCGATCATCTCTCGTTTTGACATCCTCTGTGTTGTTAAGGATGTGGTTGACCCAGTCACTGATGAAATGCTTGCAAAGTTTGTCGTTGATAGTCACTTCAAGTCACAAGCCAAGGGAGCTAACATGGATGATATGCCTATAAGCGAATCCCACGAAGATAGTGAAGCCTCTGCCAACCCAGTAGATCCAGAGATAATTCCTCAAGATATGCTGAAGAAATACATAACATATGCCAAGTTAAATGTATTCCCAAGGTTGCACGATGCTGATTTGGAAAAGATCACACGTGTTTATGCTGATATGCGGAGAGAATCTTCACATGGACAAGGAGTACCCATAGCTGTAAGGCATATTGAATCAATGATCAGAATGTCTGAAGCACATGCAAGAATGCACCTTAGACAGCATGTAATACAAGAAGACGTGGACATGGCGATTCGTGTATTACTTGATTCATTCATCTCAACACAAAAATTTGGGGTGCAGAAAGCTCTGCAAAAGAAATTCAGAGAATACATGACTTACAAGAAGGATTACAACAGTGCGGTCCTTCATTTACTCCGTCAGCTCGTGAGGGATGCAATCCGCTTTGAAGAAATTGTTTCCGGATCAGCCGCAGGGCTCACCCACATTGATGTAAAAGTGGCAGATTTGCAGAGGATGGCGCAGGAGTATGAGATTCTTGATCTGCGACCCTTCTTTACAAGCGCTAGTTTCTCAGGAGCCAACTTTGAGTTGGATGAAGAGCGTGGATTTATAAGGCATCACCTTGCAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM2_N MCM_N MCM_OB

Protein Analysis

930

Amino Acids

105.29

Weight (kDa)

5.17

Isoelectric Point (pI)

47.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM2_N	PF12619	38 - 154	1.4e-12	Mini-chromosome maintenance protein 2
MCM_N	PF14551	215 - 306	2.6e-16	MCM N-terminal domain
MCM_OB	PF17207	312 - 439	2.4e-34	MCM OB domain
MCM	PF00493	480 - 702	1.8e-104	MCM P-loop domain
AAA_5	PF07728	537 - 655	6.3e-07	AAA domain (dynein-related subfamily)
Mg_chelatase	PF01078	591 - 682	1.2e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	739 - 823	5.2e-27	MCM AAA-lid domain
WHD_MCM2	PF23669	847 - 923	1.1e-21	DNA replication licensing factor MCM2-like, winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44900 AT1G44900
fragaria_vesca	FvH4_4g22180
malus_domestica	MD13G1134100.v1.1 MD16G1144700.v1.1
prunus_persica	Prupe.1G211500_v2.0.a1
pyrus_communis	pycom13g11680 pycom16g11400
rosa_chinensis	RchiOBHm_Chr4g0428411
rosa_laevigata	RLG00000007153
rosa_multiflora	Rmu_sc0004698.1_g000003
rosa_roxburghii	Rroxscaffold_5G00370080
rosa_rugosa	Rorug04G0225200
rosa_samantha	Rh4BG288100 Rh4CG303800 Rh4DG285200
rosa_wichuraiana	Rw4G024520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1490, 2768
AatII	GACGTC	1 cut(s)	41
Acc36I	ACCTGC	2 cut(s)	943, 1208
AccI	GTMKAC	3 cut(s)	20, 129, 1691
AccII	CGCG	2 cut(s)	326, 348
AccIII	TCCGGA	1 cut(s)	2615
AclWI	GGATC	9 cut(s)	201, 306, 690, 1622, 1739, 1752, 2003, 2189, 2626
AcoI	YGGCCR	2 cut(s)	309, 1516
AcsI	RAATTY	5 cut(s)	647, 1081, 1820, 2483, 2513
AcuI	CTGAAG	3 cut(s)	1113, 2243, 2406
AcvI	CACGTG	1 cut(s)	2297
AcyI	GRCGYC	3 cut(s)	23, 38, 354
AfaI	GTAC	2 cut(s)	1614, 2343
AfeI	AGCGCT	1 cut(s)	2719
AfiI	CCNNNNNNNGG	6 cut(s)	178, 318, 325, 1221, 1475, 1958
AflII	CTTAAG	1 cut(s)	1655
AflIII	ACRYGT	2 cut(s)	2294, 2296
AjiI	CACGTC	1 cut(s)	2434
AjnI	CCWGG	3 cut(s)	1201, 1279, 1630
AjuI	GAANNNNNNNTTGG	2 cut(s)	1086, 1118
AleI	CACNNNNGTG	1 cut(s)	671
AloI	GAACNNNNNNTCC	2 cut(s)	83, 115
Alw21I	GWGCWC	1 cut(s)	1276
Alw26I	GTCTC	2 cut(s)	524, 612
AlwI	GGATC	9 cut(s)	201, 306, 690, 1622, 1739, 1752, 2003, 2189, 2626
AlwNI	CAGNNNCTG	2 cut(s)	1733, 2069
Aor13HI	TCCGGA	1 cut(s)	2615
Aor51HI	AGCGCT	1 cut(s)	2719
AoxI	GGCC	8 cut(s)	120, 309, 321, 419, 449, 1067, 1516, 1561
ApeKI	GCWGC	7 cut(s)	64, 1024, 1730, 1873, 1926, 1940, 1943
ApoI	RAATTY	5 cut(s)	647, 1081, 1820, 2483, 2513
ArsI	GACNNNNNNTTYG	2 cut(s)	13, 45
AseI	ATTAAT	1 cut(s)	714
AspLEI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
AspS9I	GGNCC	5 cut(s)	321, 955, 1067, 1133, 2557
AsuC2I	CCSGG	2 cut(s)	320, 495
AsuHPI	GGTGA	6 cut(s)	483, 658, 1637, 1861, 2626, 2768
AsuII	TTCGAA	1 cut(s)	425
AvaII	GGWCC	3 cut(s)	955, 1133, 2557
BaeGI	GKGCMC	1 cut(s)	243
BaeI	ACNNNNGTAYC	2 cut(s)	868, 901
BalI	TGGCCA	1 cut(s)	1518
BamHI	GGATCC	1 cut(s)	1744
BanII	GRGCYC	3 cut(s)	1202, 1725, 2634
BauI	CACGAG	2 cut(s)	73, 2579
BbrPI	CACGTG	1 cut(s)	2297
BbsI	GAAGAC	5 cut(s)	567, 573, 1440, 1676, 2436
Bbv12I	GWGCWC	1 cut(s)	1276
BbvI	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
BccI	CCATC	7 cut(s)	230, 387, 470, 715, 897, 1513, 2665
BceAI	ACGGC	4 cut(s)	110, 367, 559, 1653
BciT130I	CCWGG	3 cut(s)	1203, 1281, 1632
BciVI	GTATCC	1 cut(s)	896
BclI	TGATCA	1 cut(s)	2373
BcnI	CCSGG	2 cut(s)	320, 495
BcoDI	GTCTC	2 cut(s)	524, 612
BfaI	CTAG	2 cut(s)	1790, 2721
BfoI	RGCGCY	2 cut(s)	1784, 2721
BfrI	CTTAAG	1 cut(s)	1655
BfuAI	ACCTGC	2 cut(s)	943, 1208
BfuI	GTATCC	1 cut(s)	896
BglII	AGATCT	1 cut(s)	897
Bme1390I	CCNGG	5 cut(s)	320, 495, 1203, 1281, 1632
Bme18I	GGWCC	3 cut(s)	955, 1133, 2557
BmgBI	CACGTC	1 cut(s)	2434
BmgT120I	GGNCC	5 cut(s)	321, 955, 1067, 1133, 2557
BmiI	GGNNCC	3 cut(s)	1068, 1746, 2734
BmrFI	CCNGG	5 cut(s)	320, 495, 1203, 1281, 1632
BmrI	ACTGGG	5 cut(s)	808, 1012, 1683, 2057, 2183
BmsI	GCATC	5 cut(s)	796, 1718, 2263, 2578, 2782
BmuI	ACTGGG	5 cut(s)	808, 1012, 1683, 2057, 2183
BoxI	GACNNNNGTC	1 cut(s)	41
BpiI	GAAGAC	5 cut(s)	567, 573, 1440, 1676, 2436
BplI	GAGNNNNNCTC	4 cut(s)	80, 112, 180, 212
BpmI	CTGGAG	2 cut(s)	508, 767
Bpu14I	TTCGAA	1 cut(s)	425
BpuEI	CTTGAG	1 cut(s)	2196
BpuMI	CCSGG	2 cut(s)	320, 495
BsaAI	YACGTR	1 cut(s)	2297
BsaBI	GATNNNNATC	3 cut(s)	12, 222, 297
BsaHI	GRCGYC	3 cut(s)	23, 38, 354
BsaJI	CCNNGG	5 cut(s)	324, 1201, 1280, 2121, 2261
BsaWI	WCCGGW	1 cut(s)	2615
BsaXI	ACNNNNNCTCC	4 cut(s)	975, 1005, 1173, 1203
Bsc4I	CCNNNNNNNGG	6 cut(s)	178, 318, 325, 1221, 1475, 1958
Bse118I	RCCGGY	1 cut(s)	417
Bse1I	ACTGG	7 cut(s)	814, 1018, 1306, 1678, 1702, 2063, 2189
Bse3DI	GCAATG	2 cut(s)	1872, 1935
Bse8I	GATNNNNATC	3 cut(s)	12, 222, 297
BseAI	TCCGGA	1 cut(s)	2615
BseBI	CCWGG	3 cut(s)	1203, 1281, 1632
BseDI	CCNNGG	5 cut(s)	324, 1201, 1280, 2121, 2261
BseJI	GATNNNNATC	3 cut(s)	12, 222, 297
BseLI	CCNNNNNNNGG	6 cut(s)	178, 318, 325, 1221, 1475, 1958
BseMI	GCAATG	2 cut(s)	1872, 1935
BseMII	CTCAG	2 cut(s)	1442, 2742
BseNI	ACTGG	7 cut(s)	814, 1018, 1306, 1678, 1702, 2063, 2189
BseRI	GAGGAG	3 cut(s)	94, 194, 197
BseSI	GKGCMC	1 cut(s)	243
BseXI	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
BseYI	CCCAGC	1 cut(s)	1898
BsgI	GTGCAG	1 cut(s)	2513
Bsh1236I	CGCG	2 cut(s)	326, 348
Bsh1285I	CGRYCG	2 cut(s)	45, 316
BshFI	GGCC	8 cut(s)	122, 311, 323, 421, 451, 1069, 1518, 1563
BsiEI	CGRYCG	2 cut(s)	45, 316
BsiHKAI	GWGCWC	1 cut(s)	1276
BsiSI	CCGG	5 cut(s)	26, 319, 418, 495, 2616
BslFI	GGGAC	1 cut(s)	1196
BslI	CCNNNNNNNGG	6 cut(s)	178, 318, 325, 1221, 1475, 1958
BsmAI	GTCTC	2 cut(s)	524, 612
BsmBI	CGTCTC	1 cut(s)	612
BsmFI	GGGAC	1 cut(s)	1196
BsmI	GAATGC	3 cut(s)	486, 1124, 2406
BsnI	GGCC	8 cut(s)	122, 311, 323, 421, 451, 1069, 1518, 1563
Bsp119I	TTCGAA	1 cut(s)	425
Bsp1286I	GDGCHC	5 cut(s)	243, 1202, 1276, 1725, 2634
Bsp13I	TCCGGA	1 cut(s)	2615
BspANI	GGCC	8 cut(s)	122, 311, 323, 421, 451, 1069, 1518, 1563
BspCNI	CTCAG	2 cut(s)	1441, 2741
BspEI	TCCGGA	1 cut(s)	2615
BspFNI	CGCG	2 cut(s)	326, 348
BspHI	TCATGA	1 cut(s)	1858
BspLI	GGNNCC	3 cut(s)	1068, 1746, 2734
BspMI	ACCTGC	2 cut(s)	943, 1208
BspPI	GGATC	9 cut(s)	201, 306, 690, 1622, 1739, 1752, 2003, 2189, 2626
BspQI	GCTCTTC	1 cut(s)	2748
BspT104I	TTCGAA	1 cut(s)	425
BspTI	CTTAAG	1 cut(s)	1655
BsrDI	GCAATG	2 cut(s)	1872, 1935
BsrFI	RCCGGY	1 cut(s)	417
BsrI	ACTGG	7 cut(s)	814, 1018, 1306, 1678, 1702, 2063, 2189
BssAI	RCCGGY	1 cut(s)	417
BssECI	CCNNGG	5 cut(s)	324, 1201, 1280, 2121, 2261
BssNAI	GTATAC	1 cut(s)	1692
BssNI	GRCGYC	3 cut(s)	23, 38, 354
BssSI	CACGAG	2 cut(s)	73, 2579
BssT1I	CCWWGG	2 cut(s)	2121, 2261
Bst1107I	GTATAC	1 cut(s)	1692
Bst2BI	CACGAG	2 cut(s)	73, 2579
Bst2UI	CCWGG	3 cut(s)	1203, 1281, 1632
Bst4CI	ACNGT	5 cut(s)	507, 689, 1369, 1650, 2552
Bst6I	CTCTTC	7 cut(s)	112, 171, 203, 422, 1281, 1437, 2748
BstACI	GRCGYC	3 cut(s)	23, 38, 354
BstAFI	CTTAAG	1 cut(s)	1655
BstBAI	YACGTR	1 cut(s)	2297
BstBI	TTCGAA	1 cut(s)	425
BstC8I	GCNNGC	3 cut(s)	371, 1924, 2082
BstDEI	CTNAG	7 cut(s)	599, 1392, 1428, 1595, 1621, 2408, 2728
BstDSI	CCRYGG	1 cut(s)	324
BstENI	CCTNNNNNAGG	2 cut(s)	176, 1956
BstFNI	CGCG	2 cut(s)	326, 348
BstH2I	RGCGCY	2 cut(s)	1784, 2721
BstHHI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
BstMAI	GTCTC	2 cut(s)	524, 612
BstMCI	CGRYCG	2 cut(s)	45, 316
BstMWI	GCNNNNNNNGC	3 cut(s)	31, 1119, 2126
BstNI	CCWGG	3 cut(s)	1203, 1281, 1632
BstNSI	RCATGY	2 cut(s)	2396, 2420
BstPAI	GACNNNNGTC	1 cut(s)	41
BstSCI	CCNGG	5 cut(s)	318, 493, 1201, 1279, 1630
BstSLI	GKGCMC	1 cut(s)	243
BstUI	CGCG	2 cut(s)	326, 348
BstV1I	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
BstV2I	GAAGAC	5 cut(s)	567, 573, 1440, 1676, 2436
BstX2I	RGATCY	5 cut(s)	193, 897, 1744, 2008, 2194
BstXI	CCANNNNNNTGG	1 cut(s)	799
BstYI	RGATCY	5 cut(s)	193, 897, 1744, 2008, 2194
BstZ17I	GTATAC	1 cut(s)	1692
BsuI	GTATCC	1 cut(s)	896
BsuRI	GGCC	8 cut(s)	122, 311, 323, 421, 451, 1069, 1518, 1563
BtgI	CCRYGG	1 cut(s)	324
BtgZI	GCGATG	1 cut(s)	561
BtrI	CACGTC	1 cut(s)	2434
BtsIMutI	CAGTG	6 cut(s)	694, 1147, 1299, 1365, 2067, 2557
BveI	ACCTGC	2 cut(s)	943, 1208
Cac8I	GCNNGC	3 cut(s)	371, 1924, 2082
CaiI	CAGNNNCTG	2 cut(s)	1733, 2069
CciI	TCATGA	1 cut(s)	1858
CfoI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
Cfr10I	RCCGGY	1 cut(s)	417
Cfr13I	GGNCC	5 cut(s)	321, 955, 1067, 1133, 2557
Cfr42I	CCGCGG	1 cut(s)	327
CseI	GACGC	3 cut(s)	31, 343, 1008
Csp6I	GTAC	2 cut(s)	1613, 2342
CspCI	CAANNNNNGTGG	4 cut(s)	1033, 1068, 1354, 1389
CviQI	GTAC	2 cut(s)	1613, 2342
DdeI	CTNAG	7 cut(s)	599, 1392, 1428, 1595, 1621, 2408, 2728
EaeI	YGGCCR	2 cut(s)	309, 1516
Eam1104I	CTCTTC	7 cut(s)	112, 171, 203, 422, 1281, 1437, 2748
EarI	CTCTTC	7 cut(s)	112, 171, 203, 422, 1281, 1437, 2748
Eco130I	CCWWGG	2 cut(s)	2121, 2261
Eco147I	AGGCCT	1 cut(s)	451
Eco24I	GRGCYC	3 cut(s)	1202, 1725, 2634
Eco32I	GATATC	1 cut(s)	694
Eco47I	GGWCC	3 cut(s)	955, 1133, 2557
Eco47III	AGCGCT	1 cut(s)	2719
Eco57I	CTGAAG	3 cut(s)	1113, 2243, 2406
Eco72I	CACGTG	1 cut(s)	2297
EcoNI	CCTNNNNNAGG	2 cut(s)	176, 1956
EcoRI	GAATTC	1 cut(s)	1081
EcoRII	CCWGG	3 cut(s)	1201, 1279, 1630
EcoRV	GATATC	1 cut(s)	694
EcoT14I	CCWWGG	2 cut(s)	2121, 2261
EcoT38I	GRGCYC	3 cut(s)	1202, 1725, 2634
ErhI	CCWWGG	2 cut(s)	2121, 2261
Esp3I	CGTCTC	1 cut(s)	612
FalI	AAGNNNNNCTT	2 cut(s)	321, 353
FaqI	GGGAC	1 cut(s)	1196
FauI	CCCGC	3 cut(s)	353, 374, 1222
FauNDI	CATATG	1 cut(s)	2239
FbaI	TGATCA	1 cut(s)	2373
FblI	GTMKAC	3 cut(s)	20, 129, 1691
FriOI	GRGCYC	3 cut(s)	1202, 1725, 2634
FspBI	CTAG	2 cut(s)	1790, 2721
GlaI	GCGC	5 cut(s)	707, 1503, 1782, 2676, 2719
GsaI	CCCAGC	1 cut(s)	1902
GsuI	CTGGAG	2 cut(s)	508, 767
HaeII	RGCGCY	2 cut(s)	1784, 2721
HaeIII	GGCC	8 cut(s)	122, 311, 323, 421, 451, 1069, 1518, 1563
HapII	CCGG	5 cut(s)	26, 319, 418, 495, 2616
HgaI	GACGC	3 cut(s)	31, 343, 1008
HhaI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
Hin1I	GRCGYC	3 cut(s)	23, 38, 354
Hin6I	GCGC	5 cut(s)	706, 1502, 1781, 2675, 2718
HinP1I	GCGC	5 cut(s)	706, 1502, 1781, 2675, 2718
HincII	GTYRAC	5 cut(s)	21, 130, 541, 2004, 2059
HindII	GTYRAC	5 cut(s)	21, 130, 541, 2004, 2059
HpaI	GTTAAC	1 cut(s)	2004
HpaII	CCGG	5 cut(s)	26, 319, 418, 495, 2616
HphI	GGTGA	6 cut(s)	483, 658, 1637, 1861, 2626, 2768
HpyAV	CCTTC	5 cut(s)	432, 1766, 2533, 2570, 2716
HpyCH4III	ACNGT	5 cut(s)	507, 689, 1369, 1650, 2552
HpyCH4IV	ACGT	4 cut(s)	38, 883, 2296, 2433
HpyF10VI	GCNNNNNNNGC	3 cut(s)	31, 1119, 2126
HpyF3I	CTNAG	7 cut(s)	599, 1392, 1428, 1595, 1621, 2408, 2728
HpySE526I	ACGT	4 cut(s)	38, 883, 2296, 2433
Hsp92I	GRCGYC	3 cut(s)	23, 38, 354
HspAI	GCGC	5 cut(s)	706, 1502, 1781, 2675, 2718
Kpn2I	TCCGGA	1 cut(s)	2615
Ksp22I	TGATCA	1 cut(s)	2373
KspAI	GTTAAC	1 cut(s)	2004
KspI	CCGCGG	1 cut(s)	327
LguI	GCTCTTC	1 cut(s)	2748
LmnI	GCTCC	5 cut(s)	378, 1706, 1870, 2126, 2732
Lsp1109I	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
LweI	GCATC	5 cut(s)	796, 1718, 2263, 2578, 2782
MaeI	CTAG	2 cut(s)	1790, 2721
MaeII	ACGT	4 cut(s)	38, 883, 2296, 2433
MaeIII	GTNAC	9 cut(s)	507, 610, 991, 1297, 1750, 1906, 2065, 2102, 2112
MfeI	CAATTG	1 cut(s)	1497
MflI	RGATCY	5 cut(s)	193, 897, 1744, 2008, 2194
MhlI	GDGCHC	5 cut(s)	243, 1202, 1276, 1725, 2634
MlsI	TGGCCA	1 cut(s)	1518
MluNI	TGGCCA	1 cut(s)	1518
MlyI	GAGTC	2 cut(s)	263, 1759
MmeI	TCCRAC	3 cut(s)	135, 1936, 2727
Mox20I	TGGCCA	1 cut(s)	1518
MroI	TCCGGA	1 cut(s)	2615
MscI	TGGCCA	1 cut(s)	1518
MseI	TTAA	6 cut(s)	573, 714, 1656, 2003, 2046, 2249
MslI	CAYNNNNRTG	2 cut(s)	671, 2643
Msp20I	TGGCCA	1 cut(s)	1518
MspA1I	CMGCKG	4 cut(s)	64, 326, 1733, 1943
MspCI	CTTAAG	1 cut(s)	1655
MspI	CCGG	5 cut(s)	26, 319, 418, 495, 2616
MspR9I	CCNGG	5 cut(s)	320, 495, 1203, 1281, 1632
MunI	CAATTG	1 cut(s)	1497
Mva1269I	GAATGC	3 cut(s)	486, 1124, 2406
MvaI	CCWGG	3 cut(s)	1203, 1281, 1632
MvnI	CGCG	2 cut(s)	326, 348
MwoI	GCNNNNNNNGC	3 cut(s)	31, 1119, 2126
NciI	CCSGG	2 cut(s)	320, 495
NdeI	CATATG	1 cut(s)	2239
NlaIV	GGNNCC	3 cut(s)	1068, 1746, 2734
NmuCI	GTSAC	7 cut(s)	507, 991, 1297, 1906, 2065, 2102, 2112
NspI	RCATGY	2 cut(s)	2396, 2420
NspV	TTCGAA	1 cut(s)	425
OliI	CACNNNNGTG	1 cut(s)	671
PagI	TCATGA	1 cut(s)	1858
PceI	AGGCCT	1 cut(s)	451
PciSI	GCTCTTC	1 cut(s)	2748
PcsI	WCGNNNNNNNCGW	2 cut(s)	123, 249
PctI	GAATGC	3 cut(s)	486, 1124, 2406
PflFI	GACNNNGTC	2 cut(s)	353, 2063
PleI	GAGTC	2 cut(s)	262, 1759
PmaCI	CACGTG	1 cut(s)	2297
PmlI	CACGTG	1 cut(s)	2297
PpsI	GAGTC	2 cut(s)	262, 1759
Ppu21I	YACGTR	1 cut(s)	2297
PshAI	GACNNNNGTC	1 cut(s)	41
PshBI	ATTAAT	1 cut(s)	714
PsiI	TTATAA	2 cut(s)	1490, 2768
Psp6I	CCWGG	3 cut(s)	1201, 1279, 1630
PspCI	CACGTG	1 cut(s)	2297
PspFI	CCCAGC	1 cut(s)	1898
PspGI	CCWGG	3 cut(s)	1201, 1279, 1630
PspN4I	GGNNCC	3 cut(s)	1068, 1746, 2734
PspPI	GGNCC	5 cut(s)	321, 955, 1067, 1133, 2557
PstNI	CAGNNNCTG	2 cut(s)	1733, 2069
PsuI	RGATCY	5 cut(s)	193, 897, 1744, 2008, 2194
PsyI	GACNNNGTC	2 cut(s)	353, 2063
PvuII	CAGCTG	3 cut(s)	64, 1733, 1943
RsaI	GTAC	2 cut(s)	1614, 2343
RsaNI	GTAC	2 cut(s)	1613, 2342
RseI	CAYNNNNRTG	2 cut(s)	671, 2643
SacII	CCGCGG	1 cut(s)	327
SalI	GTCGAC	2 cut(s)	19, 128
SapI	GCTCTTC	1 cut(s)	2748
SaqAI	TTAA	6 cut(s)	573, 714, 1656, 2003, 2046, 2249
Sau96I	GGNCC	5 cut(s)	321, 955, 1067, 1133, 2557
SchI	GAGTC	2 cut(s)	263, 1759
ScrFI	CCNGG	5 cut(s)	320, 495, 1203, 1281, 1632
SduI	GDGCHC	5 cut(s)	243, 1202, 1276, 1725, 2634
SfaNI	GCATC	5 cut(s)	796, 1718, 2263, 2578, 2782
Sfr303I	CCGCGG	1 cut(s)	327
SfuI	TTCGAA	1 cut(s)	425
SgrBI	CCGCGG	1 cut(s)	327
SgrDI	CGTCGACG	1 cut(s)	19
SinI	GGWCC	3 cut(s)	955, 1133, 2557
SmiMI	CAYNNNNRTG	2 cut(s)	671, 2643
SmlI	CTYRAG	2 cut(s)	1655, 2211
SmoI	CTYRAG	2 cut(s)	1655, 2211
SseBI	AGGCCT	1 cut(s)	451
SspI	AATATT	2 cut(s)	787, 1150
SspMI	CTAG	2 cut(s)	1790, 2721
StuI	AGGCCT	1 cut(s)	451
StyD4I	CCNGG	5 cut(s)	318, 493, 1201, 1279, 1630
StyI	CCWWGG	2 cut(s)	2121, 2261
TaaI	ACNGT	5 cut(s)	507, 689, 1369, 1650, 2552
TaiI	ACGT	4 cut(s)	41, 886, 2299, 2436
TaqI	TCGA	5 cut(s)	20, 129, 333, 425, 696
TaqII	GACCGA	1 cut(s)	31
TatI	WGTACW	1 cut(s)	1612
TauI	GCSGC	5 cut(s)	70, 81, 125, 326, 2627
Tru1I	TTAA	6 cut(s)	573, 714, 1656, 2003, 2046, 2249
Tru9I	TTAA	6 cut(s)	573, 714, 1656, 2003, 2046, 2249
TscAI	CASTG	6 cut(s)	694, 1147, 1306, 1372, 2074, 2557
TseFI	GTSAC	7 cut(s)	507, 991, 1297, 1906, 2065, 2102, 2112
TseI	GCWGC	7 cut(s)	64, 1024, 1730, 1873, 1926, 1940, 1943
Tsp45I	GTSAC	7 cut(s)	507, 991, 1297, 1906, 2065, 2102, 2112
TspGWI	ACGGA	3 cut(s)	6, 265, 2561
TspRI	CASTG	6 cut(s)	694, 1147, 1306, 1372, 2074, 2557
Tth111I	GACNNNGTC	2 cut(s)	353, 2063
Vha464I	CTTAAG	1 cut(s)	1655
VpaK11BI	GGWCC	3 cut(s)	955, 1133, 2557
VspI	ATTAAT	1 cut(s)	714
XagI	CCTNNNNNAGG	2 cut(s)	176, 1956
XapI	RAATTY	5 cut(s)	647, 1081, 1820, 2483, 2513
XceI	RCATGY	2 cut(s)	2396, 2420
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1199, 2743
XmiI	GTMKAC	3 cut(s)	20, 129, 1691
XspI	CTAG	2 cut(s)	1790, 2721
ZraI	GACGTC	1 cut(s)	39

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.