Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0014494

Physical Location & Seq

Genomic Coordinates Reverse (-)

50364849 .. 50370401

Gene Structure 5553 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G024520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGGAAATGGCAGACAACTCCGGGAATCCGCCGTCGACGCCGGATTCACCAACGTCGGTAGGGTTCAACACCGACCAGCTGCCGCACACGAGCCGCACCTCCGAGAACTTCTCCTCCTCCGGCGACGAGGCCGACGTCGACCCCAATATCCTCCAAGATGACGTGGAGGACGACCTCCTTGTCGAAGAAGAGCCGGAGGGGGAGGATCTCCTGGAAGGAAATTACATGGAGGACTACCGGAGAATGGATGAGATGGATCAGTACGAGTCGGTGGGGCTGGATGATTCGGCTGAGGACGAGAGGGATTTGGATCAGATCATGGCGGATCGCCGGGCGGCTGAACTTGAGCTTGATAAACGCGACGGCGTACCGGCCTCTCGCAAGCTTCCTCATCTTCTTCATGACCAAGATACTGATGAGGACAACTACAGGCCTTCAAAGAGGGCGAGAGCTGACTTCAGGCCTCCGAGGAGCGCAGATGATACCGATGGAATGCCGAGTTCGCCTGGAAGATCACAGCCTGGACACTCCAGAGAGGATGTTCCCATGACTGATCAGACTGATGATCAATATGAGGATGATGAAGACGAAGATGGGTTTGACATGTATCGTGTCCAGGGAACACTCCGAGAGTGGGTTACTAGAGATGAAGTACGAAGATTTATTGCCAAGAAATTTAAAGAGTTTCTACTCACCTATACAAATCAAAGGAACGATCATGGTGACATTGAATATGTTCGCCTTATTAATGAGATGGTGTTAGCGAACAAGTGTAGTCTGGAGATTGACTACAAGCAATTCATCTATGTTCATCCCAATATTGCTATTTGGTTGGCTGATGCGCCCCAGTCTGTCCTGGAAGTCATGGAAGATGTTGCGAAGAATGTTGTGTTTAATTTACACCCCAATTACAAACTTATCCATCAAAAGATCTATGTTCGCATAACTAACTTACCAGTTTATGATCAGATTCGTAACATTAGGCAGATTCATTTGAATACCATGATTCGTATTGGAGGGGTTGTGACCCGACGCTCTGGGGTGTTTCCCCAGTTGCAACAAGTAAAGTATGATTGCAATAAATGTGGAGCAGTCTTGGGGCCATTCTTTCAGAATTCTTATTCGGAAGTTAAAGTTGGTTCTTGCCCCGAGTGTCAATCCAAAGGGCCATTTACTGTCAATATTGAGCAGACAATATACAGGAATTATCAAAAGCTTACGCTCCAGGAGAGCCCAGGAATAGTGCCTGCAGGTCGGCTTCCAAGATACAAGGAAGTGATACTATTGAATGATCTAATTGATTGTGCTCGTCCAGGGGAAGAGATTGAGGTCACTGGTATTTATTCAAACAATTTTGACTTGTCTTTGAATACAAAGAATGGTTTTCCTGTATTTGCTACTGTGGTTGAAGCAAATTATATCACGAAGAAGCAGGACCTCTTTTCTGCTTACAAACTTACTGAGGAAGACAAACAACAGATCGAAGCGTTATCTAAAGACCCAAGAATCGGAGAAAAGATTGTCAAGTCCATTGCTCCATCTATATATGGCCATGAGGACATAAAGACTGCAATAGCTCTTGCCATGTTTGGAGGCCAAGAGAAAAATGTTGAGGGAAAACACCGACTGCGAGGTGATATAAACGTACTTCTCCTAGGTGATCCAGGCACAGCAAAATCACAATTTCTTAAGTATGTTGAAAAGACTGGGCAGAGGGCTGTGTATACCACTGGTAAAGGAGCATCTGCCGTGGGGCTTACAGCAGCTGTGCACAAGGATCCAGTTACAAAGGAGTGGACCCTTGAAGGAGGAGCCCTTGTTCTAGCAGACAAGGGAATATGCCTTATTGATGAATTTGACAAAATGAATGATCAGGACAGGGTGAGTATTCATGAAGCAATGGAGCAGCAAAGTATTAGCATATCGAAAGCTGGGATTGTCACTTCTCTCCAAGCACGCTGCTCTGTCATTGCAGCTGCAAACCCTGTTGGAGGAAGATATGATTCCTCAAAAACATTTACACAAAATGTTGAGTTGACAGATCCCATCATCTCTCGTTTTGACATCCTTTGTGTTGTGAAGGATGTGGTTGACCCCGTCACTGATGAAATGCTTGCAAAGTTTGTAGTTGATAGTCATTTCAAGTCGCAACCCAAGGGGGCTAATATGGATGATATAACCTTAGACGAGTCTCAAGAAGATATCCAAGCCTCCACCAGTCCAGTAGATCCTGATATACTTCCTCAAGACTTGCTAAAGAAATACTTGACTTTTGCCAAGTTATATGTATTCCCAAGGTTGCACGATGCTGATTTGGATAAACTCTCAAATGTTTATGCTGAACTGCGGCGAGAATCTTCGCATGGACAAGGAGTACCAATAGCCGTAAGGCACATAGAATCAATGATACGAATGTCTGAAGCACATGCAAGAATGCACCTTAGACATCAAGTAACCGGAGATGATGTGGACATGGCAATTCGCGTAGCACTCGATTCATTCATCTCAACCCAAAAATTTGGAGTGCAGAAAGCTCTACAAAAGAGTTTCAAAAGGCATATCACTTTCAAGAAAGATAGGAATGAACTGCTCCTCTTTCTTCTCAAAGAGCTTGTGAAGAATGCATTGCAGTTTGAAGAAATTGTTTCTGGCTCTTCTTTAGGGCTTACCCATATTGATGTAAAAGTGGGAGATTTGCTGAACAAGGCAAACGAGCATGAAATCTATGATCTAAACCCCTTCTTTACCAGTGCTCTTTTCACACGTGCCAATTTTGTGTTGGATGAAGAGCGGGGATTGATAAGGCATCGCCTTTCAGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM2_N MCM_N MCM_OB

Protein Analysis

940

Amino Acids

106.09

Weight (kDa)

4.99

Isoelectric Point (pI)

47.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM2_N	PF12619	50 - 158	8.8e-12	Mini-chromosome maintenance protein 2
MCM_N	PF14551	224 - 317	1.6e-17	MCM N-terminal domain
MCM_OB	PF17207	323 - 450	2.2e-34	MCM OB domain
MCM	PF00493	491 - 713	6.1e-104	MCM P-loop domain
AAA_5	PF07728	548 - 666	7.5e-07	AAA domain (dynein-related subfamily)
Mg_chelatase	PF01078	602 - 693	1.1e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	750 - 833	8.9e-25	MCM AAA-lid domain
WHD_MCM2	PF23669	858 - 934	6.3e-25	DNA replication licensing factor MCM2-like, winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0014494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44900 AT1G44900
fragaria_vesca	FvH4_4g22180
malus_domestica	MD13G1134100.v1.1 MD16G1144700.v1.1
prunus_persica	Prupe.1G211500_v2.0.a1
pyrus_communis	pycom13g11680 pycom16g11400
rosa_chinensis	RchiOBHm_Chr4g0428411
rosa_laevigata	RLG00000007153
rosa_multiflora	Rmu_sc0004698.1_g000003
rosa_roxburghii	Rroxscaffold_5G00370080
rosa_rugosa	Rorug04G0225200
rosa_samantha	Rh4BG288100 Rh4CG303800 Rh4DG285200
rosa_wichuraiana	Rw4G024520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	179
AatII	GACGTC	1 cut(s)	138
Acc36I	ACCTGC	1 cut(s)	1241
AccBSI	CCGCTC	1 cut(s)	2791
AccI	GTMKAC	3 cut(s)	35, 138, 1724
AccII	CGCG	2 cut(s)	360, 2484
AciI	CCGC	7 cut(s)	29, 83, 94, 323, 335, 2347, 2791
AclWI	GGATC	9 cut(s)	213, 264, 318, 333, 1655, 1772, 1785, 2036, 2222
AcoI	YGGCCR	1 cut(s)	1549
AcsI	RAATTY	4 cut(s)	674, 1114, 1853, 2516
AcuI	CTGAAG	2 cut(s)	442, 2439
AcvI	CACGTG	1 cut(s)	2765
AcyI	GRCGYC	2 cut(s)	38, 135
AfaI	GTAC	5 cut(s)	263, 369, 654, 1647, 2376
AfiI	CCNNNNNNNGG	4 cut(s)	244, 634, 1508, 1991
AflII	CTTAAG	1 cut(s)	1688
AflIII	ACRYGT	2 cut(s)	603, 2762
AjiI	CACGTC	1 cut(s)	163
AjnI	CCWGG	9 cut(s)	210, 505, 520, 615, 855, 1224, 1234, 1312, 1663
AjuI	GAANNNNNNNTTGG	2 cut(s)	1119, 1151
AloI	GAACNNNNNNTCC	4 cut(s)	98, 130, 1803, 1835
Alw21I	GWGCWC	3 cut(s)	1309, 1773, 2755
Alw26I	GTCTC	1 cut(s)	2196
Alw44I	GTGCAC	1 cut(s)	1769
AlwI	GGATC	9 cut(s)	213, 264, 318, 333, 1655, 1772, 1785, 2036, 2222
AlwNI	CAGNNNCTG	1 cut(s)	1766
Ama87I	CYCGRG	1 cut(s)	1148
AoxI	GGCC	8 cut(s)	129, 372, 431, 461, 1100, 1166, 1549, 1594
ApaLI	GTGCAC	1 cut(s)	1769
ApeKI	GCWGC	6 cut(s)	79, 1763, 1906, 1959, 1973, 1976
ApoI	RAATTY	4 cut(s)	674, 1114, 1853, 2516
AseI	ATTAAT	1 cut(s)	747
Asp700I	GAANNNNTTC	1 cut(s)	735
AspA2I	CCTAGG	1 cut(s)	1654
AspLEI	GCGC	2 cut(s)	476, 844
AspS9I	GGNCC	4 cut(s)	1100, 1166, 1435, 1797
AsuC2I	CCSGG	2 cut(s)	22, 332
AsuHPI	GGTGA	6 cut(s)	39, 685, 734, 1646, 1670, 1894
AvaI	CYCGRG	1 cut(s)	1148
AvaII	GGWCC	2 cut(s)	1435, 1797
AvrII	CCTAGG	1 cut(s)	1654
BaeGI	GKGCMC	1 cut(s)	1773
BaeI	ACNNNNGTAYC	2 cut(s)	2399, 2432
BalI	TGGCCA	1 cut(s)	1551
BamHI	GGATCC	1 cut(s)	1777
BanII	GRGCYC	2 cut(s)	1235, 1816
BauI	CACGAG	1 cut(s)	88
BbrPI	CACGTG	1 cut(s)	2765
BbsI	GAAGAC	2 cut(s)	591, 1473
Bbv12I	GWGCWC	3 cut(s)	1309, 1773, 2755
BbvCI	CCTCAGC	1 cut(s)	291
BbvI	GCAGC	6 cut(s)	66, 1775, 1918, 1946, 1963, 1985
BccI	CCATC	7 cut(s)	247, 482, 587, 748, 930, 1546, 2054
BceAI	ACGGC	4 cut(s)	16, 379, 1733, 2369
BciT130I	CCWGG	9 cut(s)	212, 507, 522, 617, 857, 1226, 1236, 1314, 1665
BclI	TGATCA	4 cut(s)	553, 565, 964, 1870
BcnI	CCSGG	2 cut(s)	22, 332
BcoDI	GTCTC	1 cut(s)	2196
BfaI	CTAG	3 cut(s)	642, 1655, 1823
BfmI	CTRYAG	2 cut(s)	427, 1248
BfrI	CTTAAG	1 cut(s)	1688
BfuAI	ACCTGC	1 cut(s)	1241
BglII	AGATCT	1 cut(s)	930
BlnI	CCTAGG	1 cut(s)	1654
Bme18I	GGWCC	2 cut(s)	1435, 1797
BmeT110I	CYCGRG	1 cut(s)	1148
BmgBI	CACGTC	1 cut(s)	163
BmgT120I	GGNCC	4 cut(s)	1100, 1166, 1435, 1797
BmiI	GGNNCC	4 cut(s)	1101, 1779, 1799, 1813
BmrI	ACTGGG	3 cut(s)	841, 1045, 1716
BmsI	GCATC	4 cut(s)	829, 1751, 2296, 2815
BmuI	ACTGGG	3 cut(s)	841, 1045, 1716
BpiI	GAAGAC	2 cut(s)	591, 1473
BplI	GAGNNNNNCTC	2 cut(s)	95, 127
BpmI	CTGGAG	3 cut(s)	514, 800, 1208
Bpu10I	CCTNAGC	1 cut(s)	291
BpuEI	CTTGAG	3 cut(s)	365, 2178, 2229
BpuMI	CCSGG	2 cut(s)	22, 332
BsaAI	YACGTR	1 cut(s)	2765
BsaBI	GATNNNNATC	1 cut(s)	309
BsaHI	GRCGYC	2 cut(s)	38, 135
BsaJI	CCNNGG	8 cut(s)	467, 616, 1234, 1313, 1654, 1749, 2154, 2294
BsaWI	WCCGGW	2 cut(s)	237, 2456
BsaXI	ACNNNNNCTCC	8 cut(s)	98, 128, 1008, 1038, 1803, 1833, 2451, 2481
Bsc4I	CCNNNNNNNGG	4 cut(s)	244, 634, 1508, 1991
Bse118I	RCCGGY	1 cut(s)	370
Bse3DI	GCAATG	4 cut(s)	1530, 1905, 1968, 2624
Bse8I	GATNNNNATC	1 cut(s)	309
BseBI	CCWGG	9 cut(s)	212, 507, 522, 617, 857, 1226, 1236, 1314, 1665
BseDI	CCNNGG	8 cut(s)	467, 616, 1234, 1313, 1654, 1749, 2154, 2294
BseGI	GGATG	9 cut(s)	253, 286, 544, 583, 811, 2064, 2089, 2176, 2788
BseJI	GATNNNNATC	1 cut(s)	309
BseLI	CCNNNNNNNGG	4 cut(s)	244, 634, 1508, 1991
BseMI	GCAATG	4 cut(s)	1530, 1905, 1968, 2624
BseMII	CTCAG	2 cut(s)	282, 1452
BseRI	GAGGAG	5 cut(s)	103, 106, 484, 1824, 2582
BseSI	GKGCMC	1 cut(s)	1773
BseXI	GCAGC	6 cut(s)	66, 1775, 1918, 1946, 1963, 1985
BseYI	CCCAGC	1 cut(s)	1931
BsgI	GTGCAG	1 cut(s)	2546
Bsh1236I	CGCG	2 cut(s)	360, 2484
BshFI	GGCC	8 cut(s)	131, 374, 433, 463, 1102, 1168, 1551, 1596
BsiHKAI	GWGCWC	3 cut(s)	1309, 1773, 2755
BsiHKCI	CYCGRG	1 cut(s)	1148
BsiSI	CCGG	8 cut(s)	21, 41, 120, 194, 238, 331, 371, 2457
BslI	CCNNNNNNNGG	4 cut(s)	244, 634, 1508, 1991
BsmAI	GTCTC	1 cut(s)	2196
BsmI	GAATGC	3 cut(s)	498, 2439, 2626
BsnI	GGCC	8 cut(s)	131, 374, 433, 463, 1102, 1168, 1551, 1596
BsoBI	CYCGRG	1 cut(s)	1148
Bsp1286I	GDGCHC	5 cut(s)	1235, 1309, 1773, 1816, 2755
BspACI	CCGC	7 cut(s)	29, 83, 94, 323, 335, 2347, 2791
BspANI	GGCC	8 cut(s)	131, 374, 433, 463, 1102, 1168, 1551, 1596
BspCNI	CTCAG	2 cut(s)	283, 1453
BspFNI	CGCG	2 cut(s)	360, 2484
BspHI	TCATGA	2 cut(s)	400, 1891
BspLI	GGNNCC	4 cut(s)	1101, 1779, 1799, 1813
BspMAI	CTGCAG	1 cut(s)	1252
BspMI	ACCTGC	1 cut(s)	1241
BspPI	GGATC	9 cut(s)	213, 264, 318, 333, 1655, 1772, 1785, 2036, 2222
BspQI	GCTCTTC	3 cut(s)	183, 2659, 2781
BspTI	CTTAAG	1 cut(s)	1688
BsrBI	CCGCTC	1 cut(s)	2791
BsrDI	GCAATG	4 cut(s)	1530, 1905, 1968, 2624
BsrFI	RCCGGY	1 cut(s)	370
BssAI	RCCGGY	1 cut(s)	370
BssECI	CCNNGG	8 cut(s)	467, 616, 1234, 1313, 1654, 1749, 2154, 2294
BssNAI	GTATAC	1 cut(s)	1725
BssNI	GRCGYC	2 cut(s)	38, 135
BssSI	CACGAG	1 cut(s)	88
BssT1I	CCWWGG	3 cut(s)	1654, 2154, 2294
Bst1107I	GTATAC	1 cut(s)	1725
Bst2BI	CACGAG	1 cut(s)	88
Bst2UI	CCWGG	9 cut(s)	212, 507, 522, 617, 857, 1226, 1236, 1314, 1665
Bst4CI	ACNGT	2 cut(s)	1177, 1402
Bst6I	CTCTTC	4 cut(s)	183, 1314, 2659, 2781
BstACI	GRCGYC	2 cut(s)	38, 135
BstAFI	CTTAAG	1 cut(s)	1688
BstBAI	YACGTR	1 cut(s)	2765
BstC8I	GCNNGC	4 cut(s)	383, 1248, 1957, 2115
BstDEI	CTNAG	4 cut(s)	291, 1461, 2182, 2441
BstDSI	CCRYGG	1 cut(s)	1749
BstENI	CCTNNNNNAGG	1 cut(s)	1989
BstF5I	GGATG	9 cut(s)	253, 286, 544, 583, 811, 2064, 2089, 2176, 2788
BstFNI	CGCG	2 cut(s)	360, 2484
BstHHI	GCGC	2 cut(s)	476, 844
BstMAI	GTCTC	1 cut(s)	2196
BstMWI	GCNNNNNNNGC	2 cut(s)	37, 502
BstNI	CCWGG	9 cut(s)	212, 507, 522, 617, 857, 1226, 1236, 1314, 1665
BstNSI	RCATGY	2 cut(s)	607, 2429
BstSFI	CTRYAG	2 cut(s)	427, 1248
BstSLI	GKGCMC	1 cut(s)	1773
BstUI	CGCG	2 cut(s)	360, 2484
BstV1I	GCAGC	6 cut(s)	66, 1775, 1918, 1946, 1963, 1985
BstV2I	GAAGAC	2 cut(s)	591, 1473
BstX2I	RGATCY	5 cut(s)	205, 930, 1777, 2041, 2227
BstXI	CCANNNNNNTGG	1 cut(s)	2519
BstYI	RGATCY	5 cut(s)	205, 930, 1777, 2041, 2227
BstZ17I	GTATAC	1 cut(s)	1725
BsuRI	GGCC	8 cut(s)	131, 374, 433, 463, 1102, 1168, 1551, 1596
BtgI	CCRYGG	1 cut(s)	1749
BtgZI	GCGATG	1 cut(s)	2792
BtrI	CACGTC	1 cut(s)	163
BtsCI	GGATG	9 cut(s)	253, 286, 544, 583, 811, 2064, 2089, 2176, 2788
BtsIMutI	CAGTG	4 cut(s)	1332, 1728, 2100, 2755
BveI	ACCTGC	1 cut(s)	1241
Cac8I	GCNNGC	4 cut(s)	383, 1248, 1957, 2115
CaiI	CAGNNNCTG	1 cut(s)	1766
CciI	TCATGA	2 cut(s)	400, 1891
CfoI	GCGC	2 cut(s)	476, 844
Cfr10I	RCCGGY	1 cut(s)	370
Cfr13I	GGNCC	4 cut(s)	1100, 1166, 1435, 1797
CseI	GACGC	2 cut(s)	46, 1041
Csp6I	GTAC	5 cut(s)	262, 368, 653, 1646, 2375
CspCI	CAANNNNNGTGG	2 cut(s)	1066, 1101
CviQI	GTAC	5 cut(s)	262, 368, 653, 1646, 2375
DdeI	CTNAG	4 cut(s)	291, 1461, 2182, 2441
DraI	TTTAAA	1 cut(s)	679
DrdI	GACNNNNNNGTC	1 cut(s)	179
DseDI	GACNNNNNNGTC	1 cut(s)	179
EaeI	YGGCCR	1 cut(s)	1549
Eam1104I	CTCTTC	4 cut(s)	183, 1314, 2659, 2781
EarI	CTCTTC	4 cut(s)	183, 1314, 2659, 2781
EciI	GGCGGA	2 cut(s)	18, 338
Eco130I	CCWWGG	3 cut(s)	1654, 2154, 2294
Eco147I	AGGCCT	2 cut(s)	433, 463
Eco24I	GRGCYC	2 cut(s)	1235, 1816
Eco32I	GATATC	1 cut(s)	2203
Eco47I	GGWCC	2 cut(s)	1435, 1797
Eco57I	CTGAAG	2 cut(s)	442, 2439
Eco72I	CACGTG	1 cut(s)	2765
Eco88I	CYCGRG	1 cut(s)	1148
EcoNI	CCTNNNNNAGG	1 cut(s)	1989
EcoO109I	RGGNCCY	1 cut(s)	1435
EcoRI	GAATTC	1 cut(s)	1114
EcoRII	CCWGG	9 cut(s)	210, 505, 520, 615, 855, 1224, 1234, 1312, 1663
EcoRV	GATATC	1 cut(s)	2203
EcoT14I	CCWWGG	3 cut(s)	1654, 2154, 2294
EcoT22I	ATGCAT	1 cut(s)	2626
EcoT38I	GRGCYC	2 cut(s)	1235, 1816
ErhI	CCWWGG	3 cut(s)	1654, 2154, 2294
FalI	AAGNNNNNCTT	2 cut(s)	2249, 2281
FauI	CCCGC	1 cut(s)	2784
FbaI	TGATCA	4 cut(s)	553, 565, 964, 1870
FblI	GTMKAC	3 cut(s)	35, 138, 1724
FokI	GGATG	9 cut(s)	260, 293, 551, 590, 798, 2051, 2096, 2183, 2795
FriOI	GRGCYC	2 cut(s)	1235, 1816
FspBI	CTAG	3 cut(s)	642, 1655, 1823
GlaI	GCGC	2 cut(s)	475, 843
GsaI	CCCAGC	1 cut(s)	1935
GsuI	CTGGAG	3 cut(s)	514, 800, 1208
HaeIII	GGCC	8 cut(s)	131, 374, 433, 463, 1102, 1168, 1551, 1596
HapII	CCGG	8 cut(s)	21, 41, 120, 194, 238, 331, 371, 2457
HgaI	GACGC	2 cut(s)	46, 1041
HhaI	GCGC	2 cut(s)	476, 844
Hin1I	GRCGYC	2 cut(s)	38, 135
Hin6I	GCGC	2 cut(s)	474, 842
HinP1I	GCGC	2 cut(s)	474, 842
HincII	GTYRAC	4 cut(s)	36, 139, 2037, 2092
HindII	GTYRAC	4 cut(s)	36, 139, 2037, 2092
HindIII	AAGCTT	2 cut(s)	383, 1214
HpaII	CCGG	8 cut(s)	21, 41, 120, 194, 238, 331, 371, 2457
HphI	GGTGA	6 cut(s)	39, 685, 734, 1646, 1670, 1894
Hpy166II	GTNNAC	8 cut(s)	36, 139, 1725, 1771, 1797, 2037, 2092, 2470
Hpy8I	GTNNAC	8 cut(s)	36, 139, 1725, 1771, 1797, 2037, 2092, 2470
Hpy99I	CGWCG	8 cut(s)	37, 40, 58, 128, 137, 140, 365, 1035
HpyAV	CCTTC	5 cut(s)	209, 444, 1799, 2074, 2749
HpyCH4III	ACNGT	2 cut(s)	1177, 1402
HpyCH4IV	ACGT	5 cut(s)	53, 135, 162, 1644, 2764
HpyF10VI	GCNNNNNNNGC	2 cut(s)	37, 502
HpyF3I	CTNAG	4 cut(s)	291, 1461, 2182, 2441
HpySE526I	ACGT	5 cut(s)	53, 135, 162, 1644, 2764
Hsp92I	GRCGYC	2 cut(s)	38, 135
HspAI	GCGC	2 cut(s)	474, 842
Ksp22I	TGATCA	4 cut(s)	553, 565, 964, 1870
LguI	GCTCTTC	3 cut(s)	183, 2659, 2781
LmnI	GCTCC	8 cut(s)	471, 1088, 1227, 1540, 1739, 1811, 1903, 2595
Lsp1109I	GCAGC	6 cut(s)	66, 1775, 1918, 1946, 1963, 1985
LweI	GCATC	4 cut(s)	829, 1751, 2296, 2815
MaeI	CTAG	3 cut(s)	642, 1655, 1823
MaeII	ACGT	5 cut(s)	53, 135, 162, 1644, 2764
MaeIII	GTNAC	9 cut(s)	637, 722, 974, 1024, 1330, 1783, 1939, 2098, 2452
MbiI	CCGCTC	1 cut(s)	2791
MflI	RGATCY	5 cut(s)	205, 930, 1777, 2041, 2227
MhlI	GDGCHC	5 cut(s)	1235, 1309, 1773, 1816, 2755
MlsI	TGGCCA	1 cut(s)	1551
MluNI	TGGCCA	1 cut(s)	1551
MlyI	GAGTC	2 cut(s)	275, 2198
MmeI	TCCRAC	2 cut(s)	1969, 2760
Mox20I	TGGCCA	1 cut(s)	1551
Mph1103I	ATGCAT	1 cut(s)	2626
MroXI	GAANNNNTTC	1 cut(s)	735
MscI	TGGCCA	1 cut(s)	1551
MseI	TTAA	5 cut(s)	678, 747, 894, 1131, 1689
MslI	CAYNNNNRTG	1 cut(s)	2676
Msp20I	TGGCCA	1 cut(s)	1551
MspA1I	CMGCKG	3 cut(s)	79, 1766, 1976
MspCI	CTTAAG	1 cut(s)	1688
MspI	CCGG	8 cut(s)	21, 41, 120, 194, 238, 331, 371, 2457
Mva1269I	GAATGC	3 cut(s)	498, 2439, 2626
MvaI	CCWGG	9 cut(s)	212, 507, 522, 617, 857, 1226, 1236, 1314, 1665
MvnI	CGCG	2 cut(s)	360, 2484
MwoI	GCNNNNNNNGC	2 cut(s)	37, 502
NciI	CCSGG	2 cut(s)	22, 332
NlaIV	GGNNCC	4 cut(s)	1101, 1779, 1799, 1813
NmeAIII	GCCGAG	1 cut(s)	522
NmuCI	GTSAC	5 cut(s)	722, 1024, 1330, 1939, 2098
NsiI	ATGCAT	1 cut(s)	2626
NspI	RCATGY	2 cut(s)	607, 2429
PagI	TCATGA	2 cut(s)	400, 1891
PceI	AGGCCT	2 cut(s)	433, 463
PciI	ACATGT	1 cut(s)	603
PciSI	GCTCTTC	3 cut(s)	183, 2659, 2781
PcsI	WCGNNNNNNNCGW	2 cut(s)	132, 168
PctI	GAATGC	3 cut(s)	498, 2439, 2626
PdmI	GAANNNNTTC	1 cut(s)	735
PflFI	GACNNNGTC	1 cut(s)	2096
PfoI	TCCNGGA	4 cut(s)	20, 210, 855, 1224
PleI	GAGTC	2 cut(s)	274, 2197
PmaCI	CACGTG	1 cut(s)	2765
PmlI	CACGTG	1 cut(s)	2765
PpsI	GAGTC	2 cut(s)	274, 2197
Ppu21I	YACGTR	1 cut(s)	2765
PpuMI	RGGWCCY	1 cut(s)	1435
PscI	ACATGT	1 cut(s)	603
PshBI	ATTAAT	1 cut(s)	747
Psp5II	RGGWCCY	1 cut(s)	1435
Psp6I	CCWGG	9 cut(s)	210, 505, 520, 615, 855, 1224, 1234, 1312, 1663
PspCI	CACGTG	1 cut(s)	2765
PspFI	CCCAGC	1 cut(s)	1931
PspGI	CCWGG	9 cut(s)	210, 505, 520, 615, 855, 1224, 1234, 1312, 1663
PspN4I	GGNNCC	4 cut(s)	1101, 1779, 1799, 1813
PspPI	GGNCC	4 cut(s)	1100, 1166, 1435, 1797
PspPPI	RGGWCCY	1 cut(s)	1435
PstI	CTGCAG	1 cut(s)	1252
PstNI	CAGNNNCTG	1 cut(s)	1766
PsuI	RGATCY	5 cut(s)	205, 930, 1777, 2041, 2227
PsyI	GACNNNGTC	1 cut(s)	2096
PvuII	CAGCTG	3 cut(s)	79, 1766, 1976
RsaI	GTAC	5 cut(s)	263, 369, 654, 1647, 2376
RsaNI	GTAC	5 cut(s)	262, 368, 653, 1646, 2375
RseI	CAYNNNNRTG	1 cut(s)	2676
SalI	GTCGAC	2 cut(s)	34, 137
SapI	GCTCTTC	3 cut(s)	183, 2659, 2781
SaqAI	TTAA	5 cut(s)	678, 747, 894, 1131, 1689
Sau96I	GGNCC	4 cut(s)	1100, 1166, 1435, 1797
SbfI	CCTGCAGG	1 cut(s)	1252
SchI	GAGTC	2 cut(s)	275, 2198
SdaI	CCTGCAGG	1 cut(s)	1252
SduI	GDGCHC	5 cut(s)	1235, 1309, 1773, 1816, 2755
SfaNI	GCATC	4 cut(s)	829, 1751, 2296, 2815
SfcI	CTRYAG	2 cut(s)	427, 1248
SgrDI	CGTCGACG	1 cut(s)	34
SinI	GGWCC	2 cut(s)	1435, 1797
SmiMI	CAYNNNNRTG	1 cut(s)	2676
SmlI	CTYRAG	4 cut(s)	344, 1688, 2193, 2244
SmoI	CTYRAG	4 cut(s)	344, 1688, 2193, 2244
Sse8387I	CCTGCAGG	1 cut(s)	1252
SseBI	AGGCCT	2 cut(s)	433, 463
SsiI	CCGC	7 cut(s)	29, 83, 94, 323, 335, 2347, 2791
SspI	AATATT	2 cut(s)	820, 1183
SspMI	CTAG	3 cut(s)	642, 1655, 1823
StuI	AGGCCT	2 cut(s)	433, 463
StyI	CCWWGG	3 cut(s)	1654, 2154, 2294
TaaI	ACNGT	2 cut(s)	1177, 1402
TaiI	ACGT	5 cut(s)	56, 138, 165, 1647, 2767
TaqI	TCGA	6 cut(s)	35, 138, 183, 1482, 1925, 2493
TauI	GCSGC	4 cut(s)	85, 96, 338, 2350
Tru1I	TTAA	5 cut(s)	678, 747, 894, 1131, 1689
Tru9I	TTAA	5 cut(s)	678, 747, 894, 1131, 1689
TscAI	CASTG	4 cut(s)	1339, 1735, 2107, 2755
TseFI	GTSAC	5 cut(s)	722, 1024, 1330, 1939, 2098
TseI	GCWGC	6 cut(s)	79, 1763, 1906, 1959, 1973, 1976
Tsp45I	GTSAC	5 cut(s)	722, 1024, 1330, 1939, 2098
TspRI	CASTG	4 cut(s)	1339, 1735, 2107, 2755
Tth111I	GACNNNGTC	1 cut(s)	2096
Vha464I	CTTAAG	1 cut(s)	1688
VneI	GTGCAC	1 cut(s)	1769
VpaK11BI	GGWCC	2 cut(s)	1435, 1797
VspI	ATTAAT	1 cut(s)	747
XagI	CCTNNNNNAGG	1 cut(s)	1989
XapI	RAATTY	4 cut(s)	674, 1114, 1853, 2516
XceI	RCATGY	2 cut(s)	607, 2429
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2776
XmaJI	CCTAGG	1 cut(s)	1654
XmiI	GTMKAC	3 cut(s)	35, 138, 1724
XmnI	GAANNNNTTC	1 cut(s)	735
XspI	CTAG	3 cut(s)	642, 1655, 1823
ZraI	GACGTC	1 cut(s)	136
Zsp2I	ATGCAT	1 cut(s)	2626

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.