Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0014494

Physical Location & Seq

Genomic Coordinates Reverse (-)

25090407 .. 25095498

Gene Structure 5092 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g22180.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2748 bp

ATGGCAGATAACTCCGGCAACCCTCCGTCGACGCCGGACTCGCCGACGTCGGCAGGGTTCAACACCGATCAGCTCCCGCACACGAGCCGCACCTCCGAGAACTTCTCCTCCTCCGGCGACGAGGCCGACGTCGATCCCAATATCCTCCAAGATGACGTGGAGGACGATCTCCTTGTTGAAGAGGAGCCGGAGGGGGAGGATCTGTTGGAAGGAAACTACATGGATGACTACCGGAGAATGGATGAGATGGATCAGTATGAGTCGGTGGGGCTGGATGACTCGGCTGAGGACGAGAGGGATTTGGATCAGATCATGGCGGATCGCCGGGCGGCTGAGCTTGAGCTTGATAAGCGCGACGGCGTGCCGGCCTCTCGGAAGCTTCCTCATCTTCTTCATGACCAAGATACTGATGAGGACAACTACAGGCCTTCGAAGAGGGCCAGGGCTGATTTCAGGCCTCCGAGGAGCGCAGATGATGCCGATGGAATGGCGAGTTCACCTGGAAGATCACAGCCTGGACAATCCAGAGAGGATGTCCCCATGACTGATCAGACTGATGATCAATACGAGGATGATGAAGACGAAGATGGGTTTGACATGTATCGTGTCCAGGGAACACTCCGAGAGTGGGTTACTAGAGATGAAGTACGAAGATTTATTGCCAAGAAATTCAAAGAGTTTCTACTCACCTATACAAATCAGAGGAATGATCATGGTGACATTGAATATGTGCGCCTCATTAATGAAATGGTGTTAGCAAACAAGTGTAGTCTGGAGATTGATTACAAGCAATTTATCTATGTTCATCCCAATATTGCTATTTGGCTGGCTGATGCACCCCAGTCTGTCCTGGAAATCTATGTTCGCATAACTAACTTACCTGTTTATGATCAGATTCGTAACATTAGGCAGATTCATTTGAATACCATGATTCGTATCGGAGGGGTTGTGACCCGACGCACTGGAGTGTTTCCCCAGTTGCAACAAGTAAAGTATGATTGCAATAAATGTGGAGCAATTTTGGGGCCATTCTTTCAGAATTCTTATTCGGAAGTTAAAGTTGGTTCTTGCCCTGAATGTCAATCAAAAGGACCATTTACCGTCAATATTGAGCAGACAATATACAGGAACTATCAAAAGCTTACGCTACAGGAAAGCCCAGGAATAGTGCCTGCAGGTCGGCTTCCAAGATACAAGGAGGTTATATTATTGAATGATCTAATTGATTGTGCTCGTCCAGGGGAAGAGATTGAGGTCACTGGTATTTATTCAAATAATTTTGACCTGTCTTTGAATACAAAGAATGGGTTTCCTGTATTTGCCACTGTGGTTGAAGCAAATTATATCACAAAGAAGCAAGACCTCTTTTCTGCTTACAAACTTACTCAGGAAGACAAAGAACAGATTGAAGCGTTAGCTAAAGACCCAAGAATCGGGGAAAAGATTGTCAAGTCCATTGCTCCATCTATTTATGGCCATGAGGACATAAAGACTGCAATAGCTCTTGCCATGTTTGGAGGCCAAGAGAAAAATGTTGAGGGAAAACACCGATTGCGAGGTGATATAAACGTACTTCTCCTTGGTGATCCAGGCACAGCAAAATCACAGTTTCTTAAGTATGTCGAAAAGACTGGGCAGAGGGCTGTGTATACCACAGGTAAAGGAGCATCTGCTGTGGGGCTTACAGCAGCTGTGCACAAGGATCCTGTTACAAAGGAGTGGACCCTTGAAGGAGGAGCCCTTGTTCTAGCAGACAAGGGAATATGCCTTATTGATGAATTTGACAAAATGAATGATCAGGACAGGGTGAGTATTCATGAAGCAATGGAGCAGCAAAGTATTAGCATATCGAAAGCTGGGATTGTCACCTCTCTCCAAGCACGCTGCTCTGTCATTGCAGCTGCAAACCCTGTTGGAGGAAGATATGATTCCTCAAAAACATTTACACAAAATGTTGAGTTGACAGATCCGATCATCTCTCGTTTTGATATCCTTTGTGTTGTGAAGGATGTGGTTGACCCCGTCACTGATGAAATGCTTGCAAAGTTTGTAGTTGATAGTCATTTCAAGTCACAACCCAAGGGAGCCAATATGGATGATATAACCTTAGACGAGTCTCAAGAAGATATCCAAGCCTCCACCAGTCCAGTAGATCCTGATATACTTCCTCAAGACTTGCTAAAGAAGTACTTGACTTATGCAAAGTTAAATGTATTCCCAAGGTTGCATGATGCTGATTTGGATAAACTCTCAAATGTTTATGCTGAACTGCGGCGAGAATCTTCGCATGGACAAGGTGTACCAATAGCTGTAAGGCACATAGAATCAATGATACGAATGTCTGAAGCACATGCAAGAATGCACCTTAGACAACAAGTTACCGGAGATGATGTGGACATGGCAATTCGTGTGGCACTTGATTCATTCATCTCCACCCAAAAATTCGGGGTGCAGAAAGCTCTACAGAAGAGTTTCAAAAGGCATATCACTTTCAAGAAAGATAGGCATGAACTGGTCCTCTTTCTTCTCAAAGATCTGGTGAGGAATGCATTGCATTTTGAAGAAATCGTGTCCGGCTCTTCTTTAGGGCTCACCCATATTGATGTTAAAGTGTCAGATTTGCTGAACAAGGCAAATGAGCATGAAATCTATGATCTGAACCCCTTCTTTACTAGTGCTATTTTCACACGTGCCAATTTTGTGTTGGATGAGGAGAAGGGATTGATAAGGCATCGCCTATCAGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM2_N MCM_N MCM_OB

Protein Analysis

916

Amino Acids

103.0

Weight (kDa)

4.94

Isoelectric Point (pI)

47.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM2_N	PF12619	48 - 159	5.6e-12	Mini-chromosome maintenance protein 2
MCM_N	PF14551	222 - 289	6.2e-10	MCM N-terminal domain
MCM_OB	PF17207	298 - 425	4.8e-34	MCM OB domain
MCM	PF00493	466 - 688	4.1e-104	MCM P-loop domain
AAA_5	PF07728	523 - 641	7.2e-07	AAA domain (dynein-related subfamily)
Mg_chelatase	PF01078	577 - 668	1e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	725 - 808	3.7e-26	MCM AAA-lid domain
WHD_MCM2	PF23669	833 - 909	1.7e-23	DNA replication licensing factor MCM2-like, winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0014494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44900 AT1G44900
fragaria_vesca	FvH4_4g22180
malus_domestica	MD13G1134100.v1.1 MD16G1144700.v1.1
prunus_persica	Prupe.1G211500_v2.0.a1
pyrus_communis	pycom13g11680 pycom16g11400
rosa_chinensis	RchiOBHm_Chr4g0428411
rosa_laevigata	RLG00000007153
rosa_multiflora	Rmu_sc0004698.1_g000003
rosa_roxburghii	Rroxscaffold_5G00370080
rosa_rugosa	Rorug04G0225200
rosa_samantha	Rh4BG288100 Rh4CG303800 Rh4DG285200
rosa_wichuraiana	Rw4G024520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	50, 132
Acc36I	ACCTGC	1 cut(s)	1166
AccI	GTMKAC	2 cut(s)	29, 1649
AccII	CGCG	1 cut(s)	354
AciI	CCGC	5 cut(s)	77, 88, 317, 329, 2272
AcoI	YGGCCR	1 cut(s)	1474
AcsI	RAATTY	4 cut(s)	668, 1039, 1778, 2441
AcuI	CTGAAG	1 cut(s)	2364
AcvI	CACGTG	1 cut(s)	2690
AcyI	GRCGYC	3 cut(s)	32, 47, 129
AfaI	GTAC	4 cut(s)	648, 1572, 2189, 2301
AfiI	CCNNNNNNNGG	4 cut(s)	238, 628, 1433, 1916
AflII	CTTAAG	1 cut(s)	1613
AflIII	ACRYGT	2 cut(s)	597, 2687
AhlI	ACTAGT	1 cut(s)	2672
AjiI	CACGTC	1 cut(s)	157
AjnI	CCWGG	8 cut(s)	440, 499, 514, 609, 849, 1159, 1237, 1588
AjuI	GAANNNNNNNTTGG	2 cut(s)	1044, 1076
AleI	CACNNNNGTG	1 cut(s)	965
AloI	GAACNNNNNNTCC	4 cut(s)	92, 124, 1728, 1760
Alw21I	GWGCWC	2 cut(s)	1234, 1698
Alw26I	GTCTC	1 cut(s)	2121
Alw44I	GTGCAC	1 cut(s)	1694
AlwNI	CAGNNNCTG	1 cut(s)	1691
AoxI	GGCC	8 cut(s)	123, 366, 425, 438, 455, 1025, 1474, 1519
ApaLI	GTGCAC	1 cut(s)	1694
ApeKI	GCWGC	5 cut(s)	1688, 1831, 1884, 1898, 1901
ApoI	RAATTY	4 cut(s)	668, 1039, 1778, 2441
AseI	ATTAAT	1 cut(s)	741
Asp700I	GAANNNNTTC	2 cut(s)	2471, 2663
AspLEI	GCGC	3 cut(s)	354, 470, 735
AspS9I	GGNCC	5 cut(s)	438, 1025, 1091, 1722, 2515
AsuC2I	CCSGG	1 cut(s)	326
AsuHPI	GGTGA	9 cut(s)	489, 679, 728, 1571, 1595, 1819, 1858, 2551, 2584
AsuII	TTCGAA	1 cut(s)	431
AvaII	GGWCC	3 cut(s)	1091, 1722, 2515
BaeGI	GKGCMC	1 cut(s)	1698
BaeI	ACNNNNGTAYC	2 cut(s)	2324, 2357
BalI	TGGCCA	1 cut(s)	1476
BamHI	GGATCC	1 cut(s)	1702
BanII	GRGCYC	2 cut(s)	1741, 2592
BauI	CACGAG	1 cut(s)	82
BbrPI	CACGTG	1 cut(s)	2690
BbsI	GAAGAC	2 cut(s)	585, 1398
Bbv12I	GWGCWC	2 cut(s)	1234, 1698
BbvCI	CCTCAGC	1 cut(s)	285
BbvI	GCAGC	5 cut(s)	1700, 1843, 1871, 1888, 1910
BccI	CCATC	4 cut(s)	241, 476, 581, 1471
BceAI	ACGGC	1 cut(s)	373
BciT130I	CCWGG	8 cut(s)	442, 501, 516, 611, 851, 1161, 1239, 1590
BclI	TGATCA	5 cut(s)	547, 559, 709, 889, 1795
BcnI	CCSGG	1 cut(s)	326
BcoDI	GTCTC	1 cut(s)	2121
BcuI	ACTAGT	1 cut(s)	2672
BfaI	CTAG	3 cut(s)	636, 1748, 2673
BfmI	CTRYAG	4 cut(s)	421, 1148, 1173, 2462
BfrI	CTTAAG	1 cut(s)	1613
BfuAI	ACCTGC	1 cut(s)	1166
BglII	AGATCT	1 cut(s)	2533
BisI	GCNGC	8 cut(s)	88, 330, 1689, 1832, 1885, 1899, 1902, 2273
BlpI	GCTNAGC	1 cut(s)	333
BlsI	GCNGC	8 cut(s)	89, 331, 1690, 1833, 1886, 1900, 1903, 2274
BmcAI	AGTACT	1 cut(s)	2189
Bme1390I	CCNGG	9 cut(s)	326, 442, 501, 516, 611, 851, 1161, 1239, 1590
Bme18I	GGWCC	3 cut(s)	1091, 1722, 2515
BmgBI	CACGTC	1 cut(s)	157
BmgT120I	GGNCC	5 cut(s)	438, 1025, 1091, 1722, 2515
BmiI	GGNNCC	6 cut(s)	186, 1026, 1704, 1724, 1738, 2086
BmrFI	CCNGG	9 cut(s)	326, 442, 501, 516, 611, 851, 1161, 1239, 1590
BmrI	ACTGGG	3 cut(s)	835, 970, 1641
BmsI	GCATC	5 cut(s)	466, 823, 1676, 2221, 2740
BmuI	ACTGGG	3 cut(s)	835, 970, 1641
BpiI	GAAGAC	2 cut(s)	585, 1398
BplI	GAGNNNNNCTC	2 cut(s)	89, 121
BpmI	CTGGAG	2 cut(s)	794, 984
Bpu10I	CCTNAGC	1 cut(s)	285
Bpu1102I	GCTNAGC	1 cut(s)	333
Bpu14I	TTCGAA	1 cut(s)	431
BpuEI	CTTGAG	3 cut(s)	359, 2103, 2154
BpuMI	CCSGG	1 cut(s)	326
BsaAI	YACGTR	1 cut(s)	2690
BsaBI	GATNNNNATC	2 cut(s)	303, 935
BsaHI	GRCGYC	3 cut(s)	32, 47, 129
BsaJI	CCNNGG	8 cut(s)	441, 461, 610, 1159, 1238, 1579, 2079, 2219
BsaWI	WCCGGW	2 cut(s)	231, 2381
Bsc4I	CCNNNNNNNGG	4 cut(s)	238, 628, 1433, 1916
Bse118I	RCCGGY	1 cut(s)	364
Bse1I	ACTGG	8 cut(s)	841, 967, 976, 1264, 1636, 2142, 2147, 2517
Bse3DI	GCAATG	4 cut(s)	1455, 1830, 1893, 2549
Bse8I	GATNNNNATC	2 cut(s)	303, 935
BseBI	CCWGG	8 cut(s)	442, 501, 516, 611, 851, 1161, 1239, 1590
BseDI	CCNNGG	8 cut(s)	441, 461, 610, 1159, 1238, 1579, 2079, 2219
BseGI	GGATG	9 cut(s)	229, 247, 280, 538, 577, 805, 2014, 2101, 2713
BseJI	GATNNNNATC	2 cut(s)	303, 935
BseLI	CCNNNNNNNGG	4 cut(s)	238, 628, 1433, 1916
BseMI	GCAATG	4 cut(s)	1455, 1830, 1893, 2549
BseMII	CTCAG	3 cut(s)	276, 324, 1400
BseNI	ACTGG	8 cut(s)	841, 967, 976, 1264, 1636, 2142, 2147, 2517
BseRI	GAGGAG	6 cut(s)	97, 100, 197, 478, 1749, 2726
BseSI	GKGCMC	1 cut(s)	1698
BseXI	GCAGC	5 cut(s)	1700, 1843, 1871, 1888, 1910
BseYI	CCCAGC	1 cut(s)	1856
BsgI	GTGCAG	1 cut(s)	2471
Bsh1236I	CGCG	1 cut(s)	354
BshFI	GGCC	8 cut(s)	125, 368, 427, 440, 457, 1027, 1476, 1521
BsiHKAI	GWGCWC	2 cut(s)	1234, 1698
BsiSI	CCGG	9 cut(s)	15, 35, 114, 188, 232, 325, 365, 2382, 2574
BslFI	GGGAC	1 cut(s)	521
BslI	CCNNNNNNNGG	4 cut(s)	238, 628, 1433, 1916
BsmAI	GTCTC	1 cut(s)	2121
BsmFI	GGGAC	1 cut(s)	521
BsmI	GAATGC	2 cut(s)	2364, 2551
BsnI	GGCC	8 cut(s)	125, 368, 427, 440, 457, 1027, 1476, 1521
Bsp119I	TTCGAA	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	1234, 1698, 1741, 2592
Bsp1720I	GCTNAGC	1 cut(s)	333
BspACI	CCGC	5 cut(s)	77, 88, 317, 329, 2272
BspANI	GGCC	8 cut(s)	125, 368, 427, 440, 457, 1027, 1476, 1521
BspCNI	CTCAG	3 cut(s)	277, 325, 1399
BspFNI	CGCG	1 cut(s)	354
BspHI	TCATGA	2 cut(s)	394, 1816
BspLI	GGNNCC	6 cut(s)	186, 1026, 1704, 1724, 1738, 2086
BspMAI	CTGCAG	1 cut(s)	1177
BspMI	ACCTGC	1 cut(s)	1166
BspQI	GCTCTTC	1 cut(s)	2584
BspT104I	TTCGAA	1 cut(s)	431
BspTI	CTTAAG	1 cut(s)	1613
BsrDI	GCAATG	4 cut(s)	1455, 1830, 1893, 2549
BsrFI	RCCGGY	1 cut(s)	364
BsrI	ACTGG	8 cut(s)	841, 967, 976, 1264, 1636, 2142, 2147, 2517
BssAI	RCCGGY	1 cut(s)	364
BssECI	CCNNGG	8 cut(s)	441, 461, 610, 1159, 1238, 1579, 2079, 2219
BssNAI	GTATAC	1 cut(s)	1650
BssNI	GRCGYC	3 cut(s)	32, 47, 129
BssSI	CACGAG	1 cut(s)	82
BssT1I	CCWWGG	3 cut(s)	1579, 2079, 2219
Bst1107I	GTATAC	1 cut(s)	1650
Bst2BI	CACGAG	1 cut(s)	82
Bst2UI	CCWGG	8 cut(s)	442, 501, 516, 611, 851, 1161, 1239, 1590
Bst4CI	ACNGT	3 cut(s)	1102, 1327, 1608
Bst6I	CTCTTC	5 cut(s)	174, 428, 1239, 2462, 2584
BstACI	GRCGYC	3 cut(s)	32, 47, 129
BstAFI	CTTAAG	1 cut(s)	1613
BstAPI	GCANNNNNTGC	1 cut(s)	476
BstBAI	YACGTR	1 cut(s)	2690
BstBI	TTCGAA	1 cut(s)	431
BstC8I	GCNNGC	6 cut(s)	362, 366, 828, 1173, 1882, 2040
BstDEI	CTNAG	5 cut(s)	285, 333, 1386, 2107, 2366
BstENI	CCTNNNNNAGG	1 cut(s)	1914
BstF5I	GGATG	9 cut(s)	229, 247, 280, 538, 577, 805, 2014, 2101, 2713
BstFNI	CGCG	1 cut(s)	354
BstHHI	GCGC	3 cut(s)	354, 470, 735
BstMAI	GTCTC	1 cut(s)	2121
BstMWI	GCNNNNNNNGC	3 cut(s)	40, 349, 476
BstNI	CCWGG	8 cut(s)	442, 501, 516, 611, 851, 1161, 1239, 1590
BstNSI	RCATGY	2 cut(s)	601, 2354
BstSCI	CCNGG	9 cut(s)	324, 440, 499, 514, 609, 849, 1159, 1237, 1588
BstSFI	CTRYAG	4 cut(s)	421, 1148, 1173, 2462
BstSLI	GKGCMC	1 cut(s)	1698
BstUI	CGCG	1 cut(s)	354
BstV1I	GCAGC	5 cut(s)	1700, 1843, 1871, 1888, 1910
BstV2I	GAAGAC	2 cut(s)	585, 1398
BstX2I	RGATCY	5 cut(s)	199, 1702, 1966, 2152, 2533
BstYI	RGATCY	5 cut(s)	199, 1702, 1966, 2152, 2533
BstZ17I	GTATAC	1 cut(s)	1650
BsuRI	GGCC	8 cut(s)	125, 368, 427, 440, 457, 1027, 1476, 1521
BtgZI	GCGATG	1 cut(s)	2717
BtrI	CACGTC	1 cut(s)	157
BtsCI	GGATG	9 cut(s)	229, 247, 280, 538, 577, 805, 2014, 2101, 2713
BtsIMutI	CAGTG	4 cut(s)	960, 1257, 1323, 2025
BveI	ACCTGC	1 cut(s)	1166
Cac8I	GCNNGC	6 cut(s)	362, 366, 828, 1173, 1882, 2040
CaiI	CAGNNNCTG	1 cut(s)	1691
CciI	TCATGA	2 cut(s)	394, 1816
CfoI	GCGC	3 cut(s)	354, 470, 735
Cfr10I	RCCGGY	1 cut(s)	364
Cfr13I	GGNCC	5 cut(s)	438, 1025, 1091, 1722, 2515
CseI	GACGC	2 cut(s)	40, 966
Csp6I	GTAC	4 cut(s)	647, 1571, 2188, 2300
CspCI	CAANNNNNGTGG	6 cut(s)	991, 1026, 1312, 1347, 2391, 2426
CviQI	GTAC	4 cut(s)	647, 1571, 2188, 2300
DdeI	CTNAG	5 cut(s)	285, 333, 1386, 2107, 2366
EaeI	YGGCCR	1 cut(s)	1474
Eam1104I	CTCTTC	5 cut(s)	174, 428, 1239, 2462, 2584
EarI	CTCTTC	5 cut(s)	174, 428, 1239, 2462, 2584
EciI	GGCGGA	1 cut(s)	332
Eco130I	CCWWGG	3 cut(s)	1579, 2079, 2219
Eco147I	AGGCCT	2 cut(s)	427, 457
Eco24I	GRGCYC	2 cut(s)	1741, 2592
Eco32I	GATATC	2 cut(s)	1990, 2128
Eco47I	GGWCC	3 cut(s)	1091, 1722, 2515
Eco57I	CTGAAG	1 cut(s)	2364
Eco72I	CACGTG	1 cut(s)	2690
EcoNI	CCTNNNNNAGG	1 cut(s)	1914
EcoRI	GAATTC	1 cut(s)	1039
EcoRII	CCWGG	8 cut(s)	440, 499, 514, 609, 849, 1159, 1237, 1588
EcoRV	GATATC	2 cut(s)	1990, 2128
EcoT14I	CCWWGG	3 cut(s)	1579, 2079, 2219
EcoT22I	ATGCAT	1 cut(s)	2551
EcoT38I	GRGCYC	2 cut(s)	1741, 2592
ErhI	CCWWGG	3 cut(s)	1579, 2079, 2219
FalI	AAGNNNNNCTT	2 cut(s)	2174, 2206
FaqI	GGGAC	1 cut(s)	521
FauI	CCCGC	1 cut(s)	84
FbaI	TGATCA	5 cut(s)	547, 559, 709, 889, 1795
FblI	GTMKAC	2 cut(s)	29, 1649
Fnu4HI	GCNGC	8 cut(s)	88, 330, 1689, 1832, 1885, 1899, 1902, 2273
FokI	GGATG	9 cut(s)	236, 254, 287, 545, 584, 792, 2021, 2108, 2720
FriOI	GRGCYC	2 cut(s)	1741, 2592
Fsp4HI	GCNGC	8 cut(s)	88, 330, 1689, 1832, 1885, 1899, 1902, 2273
FspBI	CTAG	3 cut(s)	636, 1748, 2673
GlaI	GCGC	3 cut(s)	353, 469, 734
GluI	GCNGC	8 cut(s)	88, 330, 1689, 1832, 1885, 1899, 1902, 2273
GsaI	CCCAGC	1 cut(s)	1860
GsuI	CTGGAG	2 cut(s)	794, 984
HaeIII	GGCC	8 cut(s)	125, 368, 427, 440, 457, 1027, 1476, 1521
HapII	CCGG	9 cut(s)	15, 35, 114, 188, 232, 325, 365, 2382, 2574
HgaI	GACGC	2 cut(s)	40, 966
HhaI	GCGC	3 cut(s)	354, 470, 735
Hin1I	GRCGYC	3 cut(s)	32, 47, 129
Hin6I	GCGC	3 cut(s)	352, 468, 733
HinP1I	GCGC	3 cut(s)	352, 468, 733
HincII	GTYRAC	3 cut(s)	30, 1962, 2017
HindII	GTYRAC	3 cut(s)	30, 1962, 2017
HindIII	AAGCTT	2 cut(s)	377, 1139
HpaII	CCGG	9 cut(s)	15, 35, 114, 188, 232, 325, 365, 2382, 2574
HphI	GGTGA	9 cut(s)	489, 679, 728, 1571, 1595, 1819, 1858, 2551, 2584
Hpy166II	GTNNAC	9 cut(s)	30, 497, 1650, 1696, 1722, 1962, 2017, 2300, 2395
Hpy8I	GTNNAC	9 cut(s)	30, 497, 1650, 1696, 1722, 1962, 2017, 2300, 2395
Hpy99I	CGWCG	9 cut(s)	31, 34, 49, 52, 122, 131, 134, 359, 960
HpyAV	CCTTC	6 cut(s)	203, 438, 1724, 1999, 2674, 2710
HpyCH4III	ACNGT	3 cut(s)	1102, 1327, 1608
HpyCH4IV	ACGT	5 cut(s)	47, 129, 156, 1569, 2689
HpyF10VI	GCNNNNNNNGC	3 cut(s)	40, 349, 476
HpyF3I	CTNAG	5 cut(s)	285, 333, 1386, 2107, 2366
HpySE526I	ACGT	5 cut(s)	47, 129, 156, 1569, 2689
Hsp92I	GRCGYC	3 cut(s)	32, 47, 129
HspAI	GCGC	3 cut(s)	352, 468, 733
KroI	GCCGGC	1 cut(s)	364
KroNI	GCCGGC	1 cut(s)	366
Ksp22I	TGATCA	5 cut(s)	547, 559, 709, 889, 1795
LguI	GCTCTTC	1 cut(s)	2584
LmnI	GCTCC	9 cut(s)	78, 184, 465, 1013, 1465, 1664, 1736, 1828, 2084
Lsp1109I	GCAGC	5 cut(s)	1700, 1843, 1871, 1888, 1910
LweI	GCATC	5 cut(s)	466, 823, 1676, 2221, 2740
MaeI	CTAG	3 cut(s)	636, 1748, 2673
MaeII	ACGT	5 cut(s)	47, 129, 156, 1569, 2689
MflI	RGATCY	5 cut(s)	199, 1702, 1966, 2152, 2533
MhlI	GDGCHC	4 cut(s)	1234, 1698, 1741, 2592
MlsI	TGGCCA	1 cut(s)	1476
MluNI	TGGCCA	1 cut(s)	1476
MlyI	GAGTC	4 cut(s)	32, 269, 272, 2123
MmeI	TCCRAC	3 cut(s)	186, 1894, 2685
Mox20I	TGGCCA	1 cut(s)	1476
Mph1103I	ATGCAT	1 cut(s)	2551
MroNI	GCCGGC	1 cut(s)	364
MroXI	GAANNNNTTC	2 cut(s)	2471, 2663
MscI	TGGCCA	1 cut(s)	1476
MseI	TTAA	5 cut(s)	741, 1056, 1614, 2207, 2607
MslI	CAYNNNNRTG	2 cut(s)	965, 2601
Msp20I	TGGCCA	1 cut(s)	1476
MspA1I	CMGCKG	2 cut(s)	1691, 1901
MspCI	CTTAAG	1 cut(s)	1613
MspI	CCGG	9 cut(s)	15, 35, 114, 188, 232, 325, 365, 2382, 2574
MspR9I	CCNGG	9 cut(s)	326, 442, 501, 516, 611, 851, 1161, 1239, 1590
Mva1269I	GAATGC	2 cut(s)	2364, 2551
MvaI	CCWGG	8 cut(s)	442, 501, 516, 611, 851, 1161, 1239, 1590
MvnI	CGCG	1 cut(s)	354
MwoI	GCNNNNNNNGC	3 cut(s)	40, 349, 476
NaeI	GCCGGC	1 cut(s)	366
NciI	CCSGG	1 cut(s)	326
NgoMIV	GCCGGC	1 cut(s)	364
NlaIV	GGNNCC	6 cut(s)	186, 1026, 1704, 1724, 1738, 2086
NmeAIII	GCCGAG	1 cut(s)	260
NmuCI	GTSAC	6 cut(s)	716, 949, 1255, 1864, 2023, 2070
NsiI	ATGCAT	1 cut(s)	2551
NspI	RCATGY	2 cut(s)	601, 2354
NspV	TTCGAA	1 cut(s)	431
OliI	CACNNNNGTG	1 cut(s)	965
PagI	TCATGA	2 cut(s)	394, 1816
PceI	AGGCCT	2 cut(s)	427, 457
PciI	ACATGT	1 cut(s)	597
PciSI	GCTCTTC	1 cut(s)	2584
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 162
PctI	GAATGC	2 cut(s)	2364, 2551
PdiI	GCCGGC	1 cut(s)	366
PdmI	GAANNNNTTC	2 cut(s)	2471, 2663
PfeI	GAWTC	8 cut(s)	895, 913, 931, 1431, 1928, 2279, 2324, 2420
PflFI	GACNNNGTC	1 cut(s)	2021
PfoI	TCCNGGA	1 cut(s)	849
PkrI	GCNGC	8 cut(s)	89, 331, 1690, 1833, 1886, 1900, 1903, 2274
PleI	GAGTC	4 cut(s)	32, 268, 272, 2122
PmaCI	CACGTG	1 cut(s)	2690
PmlI	CACGTG	1 cut(s)	2690
PpsI	GAGTC	4 cut(s)	32, 268, 272, 2122
Ppu21I	YACGTR	1 cut(s)	2690
PscI	ACATGT	1 cut(s)	597
PshBI	ATTAAT	1 cut(s)	741
Psp6I	CCWGG	8 cut(s)	440, 499, 514, 609, 849, 1159, 1237, 1588
PspCI	CACGTG	1 cut(s)	2690
PspFI	CCCAGC	1 cut(s)	1856
PspGI	CCWGG	8 cut(s)	440, 499, 514, 609, 849, 1159, 1237, 1588
PspN4I	GGNNCC	6 cut(s)	186, 1026, 1704, 1724, 1738, 2086
PspPI	GGNCC	5 cut(s)	438, 1025, 1091, 1722, 2515
PstI	CTGCAG	1 cut(s)	1177
PstNI	CAGNNNCTG	1 cut(s)	1691
PsuI	RGATCY	5 cut(s)	199, 1702, 1966, 2152, 2533
PsyI	GACNNNGTC	1 cut(s)	2021
PvuII	CAGCTG	2 cut(s)	1691, 1901
RsaI	GTAC	4 cut(s)	648, 1572, 2189, 2301
RsaNI	GTAC	4 cut(s)	647, 1571, 2188, 2300
RseI	CAYNNNNRTG	2 cut(s)	965, 2601
SalI	GTCGAC	1 cut(s)	28
SapI	GCTCTTC	1 cut(s)	2584
SaqAI	TTAA	5 cut(s)	741, 1056, 1614, 2207, 2607
SatI	GCNGC	8 cut(s)	88, 330, 1689, 1832, 1885, 1899, 1902, 2273
Sau96I	GGNCC	5 cut(s)	438, 1025, 1091, 1722, 2515
SbfI	CCTGCAGG	1 cut(s)	1177
ScaI	AGTACT	1 cut(s)	2189
SchI	GAGTC	4 cut(s)	32, 269, 272, 2123
ScrFI	CCNGG	9 cut(s)	326, 442, 501, 516, 611, 851, 1161, 1239, 1590
SdaI	CCTGCAGG	1 cut(s)	1177
SduI	GDGCHC	4 cut(s)	1234, 1698, 1741, 2592
SfaNI	GCATC	5 cut(s)	466, 823, 1676, 2221, 2740
SfcI	CTRYAG	4 cut(s)	421, 1148, 1173, 2462
SfuI	TTCGAA	1 cut(s)	431
SgrDI	CGTCGACG	1 cut(s)	28
SinI	GGWCC	3 cut(s)	1091, 1722, 2515
SmiMI	CAYNNNNRTG	2 cut(s)	965, 2601
SmlI	CTYRAG	4 cut(s)	338, 1613, 2118, 2169
SmoI	CTYRAG	4 cut(s)	338, 1613, 2118, 2169
SpeI	ACTAGT	1 cut(s)	2672
Sse8387I	CCTGCAGG	1 cut(s)	1177
SseBI	AGGCCT	2 cut(s)	427, 457
SsiI	CCGC	5 cut(s)	77, 88, 317, 329, 2272
SspI	AATATT	2 cut(s)	814, 1108
SspMI	CTAG	3 cut(s)	636, 1748, 2673
StuI	AGGCCT	2 cut(s)	427, 457
StyD4I	CCNGG	9 cut(s)	324, 440, 499, 514, 609, 849, 1159, 1237, 1588
StyI	CCWWGG	3 cut(s)	1579, 2079, 2219
TaaI	ACNGT	3 cut(s)	1102, 1327, 1608
TaiI	ACGT	5 cut(s)	50, 132, 159, 1572, 2692
TaqI	TCGA	5 cut(s)	29, 132, 431, 1623, 1850
TatI	WGTACW	1 cut(s)	2187
TauI	GCSGC	3 cut(s)	90, 332, 2275
TfiI	GAWTC	8 cut(s)	895, 913, 931, 1431, 1928, 2279, 2324, 2420
Tru1I	TTAA	5 cut(s)	741, 1056, 1614, 2207, 2607
Tru9I	TTAA	5 cut(s)	741, 1056, 1614, 2207, 2607
TscAI	CASTG	4 cut(s)	967, 1264, 1330, 2032
TseFI	GTSAC	6 cut(s)	716, 949, 1255, 1864, 2023, 2070
TseI	GCWGC	5 cut(s)	1688, 1831, 1884, 1898, 1901
Tsp45I	GTSAC	6 cut(s)	716, 949, 1255, 1864, 2023, 2070
TspGWI	ACGGA	1 cut(s)	15
TspRI	CASTG	4 cut(s)	967, 1264, 1330, 2032
Tth111I	GACNNNGTC	1 cut(s)	2021
Vha464I	CTTAAG	1 cut(s)	1613
VneI	GTGCAC	1 cut(s)	1694
VpaK11BI	GGWCC	3 cut(s)	1091, 1722, 2515
VspI	ATTAAT	1 cut(s)	741
XagI	CCTNNNNNAGG	1 cut(s)	1914
XapI	RAATTY	4 cut(s)	668, 1039, 1778, 2441
XceI	RCATGY	2 cut(s)	601, 2354
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2701
XmiI	GTMKAC	2 cut(s)	29, 1649
XmnI	GAANNNNTTC	2 cut(s)	2471, 2663
XspI	CTAG	3 cut(s)	636, 1748, 2673
ZraI	GACGTC	2 cut(s)	48, 130
ZrmI	AGTACT	1 cut(s)	2189
Zsp2I	ATGCAT	1 cut(s)	2551

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.