Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014494

Physical Location & Seq

Genomic Coordinates Forward (+)

11131028 .. 11136384

Gene Structure 5357 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1144700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGGCAGAGAATCATCCGTCGACGCCGGATTCGCCGACGTCAGTCGGGTTCAACACGGATCAGCTGCCTCACACGAGCCGCACCTCCGAGAACTTCTCGTCCTCCTCTTCCGACGACGAGGCCGCCGTCGACCCCGAAATCGTCCGAGATGAACCCGACGACGATCCCCTCCTTGAAGAGGAGGAGGGAGAGGATCTCTTCCACGACAATTTTTTGGATGATTATCGGAGAATGGATGGGCACGACCAGTACGAGTCGGTGGGGCTGGATGACTCCGTTGAGGACGAAAGGGATTTGGATCAGATCATGGCTGACCGCCGTGCCGCGGAAGTCGAGCTCGATACCCGTGACGGCGTCCACACCTCACGCAAGCTCCCCCATCTTCTCCATGACCAAGATACTGACGATGACAGCTACCGGCCTTCGAAGAGGGCAAGGGCGGATTTCAGGCCTCCGAGAAGCTACGATGATACAGATGGAATGCAGAGTTCACCCGGAAGATCACAGTCACAGCCCGAACACTCCAGAGAGACTGATGATGACGGCGATGAGAATGATGAAGAAGAAGAATTTGAAATGTATCGTGTTCAAGGAACACTCAGAGAGTGGGTTACTAGAGACGAAGTGCGAAGATTTATAGGCAAGAAATTCAAAGAGTTTTTACTCACCTATGTGAAAAAGGATCACAATGATATCGAATGTGTGCGCCTCATTAATGAGATGGTGTTAGCCAATAAGTGTAGTCTGGAGATTGATTACAAGCAATTCATCCATGTTCATCCCAATATTGCCATTTGGTTGGCTGATGCTCCCCAGTCTGTCCTGGAAGTTATGGAAGATGTTGCCAAGAATGTTGTGTTTACTTTGCACCCCAATTACAAACGTATCCATCAAAAAATCTATGTTCGGATAACTAACTTACCTGTTTATGATCAGATTCGCAACATTAGGCAGGTCCATTTGAATACCATGATTCGTATTGGAGGGGTTGTGACCCGACGCTCTGGGGTCTTTCCCCAATTGCAGCAAGTAAAGTATGATTGCAATAAATGTGGGGCAATCTTGGGGCCATTCTTTCAGAATTCTTATTCAGAAGTGAAAGTTGGTTCTTGCCCTGAATGCCAATCAAAAGGACCATTCAGTGTCAATATTGAGCAGACAATATACAGGAATTATCAAAAGCTTACACTCCAAGAGAGCCCTGGAATTGTCCCCGCAGGTCGGCTTCCAAGATACAAGGAAGTGATACTATTGAATGATCTAATTGATTGTGCTCGTCCAGGGGAAGAGATTGAGGTCACTGGCATTTATTCAAATAATTTTGACTTGTCTTTGAATACAAAGAACGGGTTTCCTGTATTTGCCACTGTGGTTGAAGCAAATTATATCACTAAGAAGCAAGACCTCTTTTCTGCTTACAAACTTACTCAGGAAGACAAAGAAGAGATAGAAAAGTTGTCTAAGGACCCAAGAATAGGAGAAAGGCTTATCAAGTCTATTGCGCCATCAATCTATGGCCATGAGGACATAAAGACTGCAATAGCTCTTGCCATGTTTGGAGGCCAAGAGAAAAATGTTGAGGGAAAGCACAGACTAAGAGGGGATATAAATGTTCTTCTCTTAGGTGATCCTGGCACGGCAAAATCACAGTTTCTTAAGTATGTTGAAAAGACTGGGCAGAGGGCGGTGTATACTACTGGAAAAGGAGCATCTGCTGTGGGGCTCACAGCAGCTGTCCACAAGGATCCTGTTACAAGGGAGTGGACTCTTGAAGGAGGAGCGCTTGTTCTAGCTGACAAAGGAATATGTCTTATTGATGAATTTGACAAAATGAACGATCAGGATAGGGTGAGTATTCATGAAGCAATGGAGCAGCAAAGTATTAGCATATCAAAAGCTGGGATTGTCACTTCTCTACAAGCACGCTGCTCTGTCATTGCAGCTGCAAACCCTGTTGGAGGAAGATATGATTCCTCTAAAACATTTGCACAAAATGTTGAGTTAACAGATCCAATCATCTCTCGTTTTGACATACTCTGTGTTGTTAAGGATGTGGTTGACCCAGTCACTGATGAAATGCTTGCAAAGTTTGTCGTTGATAGTCACTTCAAGTCACAAGCCAAGGGAGCTAACATGGATGATATGCCTATAAGCGAATCCCACGAAGACAGTGAAGCCTCTGCCAACCCAGTAGATCCAGAGATAATTCCTCAAGATATGCTGAAGAAATACGTAACATATGCCAAGTTAAATGTATTCCCAAGGTTGCACGATGCTGATTTGGAAAAGATCACACGTGTTTATGCTGATATGCGGAGAGAATCTTCACATGGACAAGGCGTACCCATAGCTGTAAGGCACATTGAATCAATGATACGAATGTCTGAAGCACATGCAAGAATGCACCTTAGACAGCATGTTATACAAGAAGACGTGGACATGGCAATTCGTGTATTACTCGATTCATTCATCTCAACCCAAAAATTTGGGGTGCAGAAAGCTCTGCAAAAGAAATTCAGAGAATACATGACTTATAAGAAGGATTACAACAGTGCGGTCCTTCATTTACTCCGTCAGCTCGTGAGGGATGCAATCCGCTTTGAAGAAATTGTTTCTGGCTCTGCCTCAGGGCTCACCCACATTGATGTAAAAGTGGCAGATTTGCAGAGGATGGCGCAGGAGTATGAGATCCTTGATCTGCAACCCTTCTTCACAAGCGCTAGTTTCTCAGGAGCCAACTTTGAGTTGGATGAAGAGCGTGGATTGATAAGGCATCACCTTGCAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006275 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030174 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032991 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051276 GO:0060255 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko03030 ko04110 ko04111 ko04113 map03030 map04110 map04111 map04113

Pfam Domains

Protein Families

MCM MCM2_N MCM_N MCM_OB

Protein Analysis

930

Amino Acids

105.37

Weight (kDa)

5.16

Isoelectric Point (pI)

48.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM2_N	PF12619	38 - 159	1.4e-12	Mini-chromosome maintenance protein 2
MCM_N	PF14551	215 - 306	1.7e-16	MCM N-terminal domain
MCM_OB	PF17207	312 - 439	2.4e-34	MCM OB domain
MCM	PF00493	480 - 702	1.8e-104	MCM P-loop domain
AAA_5	PF07728	537 - 655	6.3e-07	AAA domain (dynein-related subfamily)
Mg_chelatase	PF01078	591 - 682	1.2e-06	Magnesium chelatase, subunit ChlI
MCM_lid	PF17855	739 - 823	1e-26	MCM AAA-lid domain
WHD_MCM2	PF23669	847 - 923	6.1e-22	DNA replication licensing factor MCM2-like, winged-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014494)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44900 AT1G44900
fragaria_vesca	FvH4_4g22180
malus_domestica	MD13G1134100.v1.1 MD16G1144700.v1.1
prunus_persica	Prupe.1G211500_v2.0.a1
pyrus_communis	pycom13g11680 pycom16g11400
rosa_chinensis	RchiOBHm_Chr4g0428411
rosa_laevigata	RLG00000007153
rosa_multiflora	Rmu_sc0004698.1_g000003
rosa_roxburghii	Rroxscaffold_5G00370080
rosa_rugosa	Rorug04G0225200
rosa_samantha	Rh4BG288100 Rh4CG303800 Rh4DG285200
rosa_wichuraiana	Rw4G024520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2535
AatII	GACGTC	1 cut(s)	41
Acc36I	ACCTGC	2 cut(s)	943, 1208
AccI	GTMKAC	3 cut(s)	20, 129, 1691
AccII	CGCG	1 cut(s)	326
AcoI	YGGCCR	1 cut(s)	1516
AcsI	RAATTY	6 cut(s)	569, 647, 1081, 1820, 2483, 2513
AcuI	CTGAAG	2 cut(s)	2243, 2406
AcvI	CACGTG	1 cut(s)	2297
AcyI	GRCGYC	3 cut(s)	23, 38, 354
AfaI	GTAC	2 cut(s)	251, 2343
AfeI	AGCGCT	2 cut(s)	1782, 2719
AfiI	CCNNNNNNNGG	5 cut(s)	178, 325, 1221, 1475, 1958
AflII	CTTAAG	1 cut(s)	1655
AflIII	ACRYGT	2 cut(s)	2294, 2296
AjiI	CACGTC	1 cut(s)	2434
AjnI	CCWGG	4 cut(s)	822, 1201, 1279, 1630
AjuI	GAANNNNNNNTTGG	2 cut(s)	1086, 1118
AleI	CACNNNNGTG	1 cut(s)	671
AloI	GAACNNNNNNTCC	4 cut(s)	83, 115, 1770, 1802
Alw21I	GWGCWC	2 cut(s)	339, 1276
Alw26I	GTCTC	2 cut(s)	524, 612
AlwNI	CAGNNNCTG	2 cut(s)	1733, 2069
Aor51HI	AGCGCT	2 cut(s)	1782, 2719
AoxI	GGCC	6 cut(s)	120, 419, 449, 1067, 1516, 1561
ApeKI	GCWGC	7 cut(s)	64, 1024, 1730, 1873, 1926, 1940, 1943
ApoI	RAATTY	6 cut(s)	569, 647, 1081, 1820, 2483, 2513
ArsI	GACNNNNNNTTYG	2 cut(s)	13, 45
AseI	ATTAAT	1 cut(s)	714
AspLEI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
AspS9I	GGNCC	5 cut(s)	955, 1067, 1133, 1465, 2557
AsuC2I	CCSGG	1 cut(s)	495
AsuHPI	GGTGA	6 cut(s)	483, 658, 1637, 1861, 2626, 2768
AsuII	TTCGAA	1 cut(s)	425
AvaII	GGWCC	4 cut(s)	955, 1133, 1465, 2557
AxyI	CCTNAGG	1 cut(s)	2626
BaeGI	GKGCMC	1 cut(s)	243
BaeI	ACNNNNGTAYC	4 cut(s)	868, 901, 2366, 2399
BalI	TGGCCA	1 cut(s)	1518
BamHI	GGATCC	1 cut(s)	1744
BanII	GRGCYC	4 cut(s)	339, 1202, 1725, 2634
BauI	CACGAG	2 cut(s)	73, 2579
BbrPI	CACGTG	1 cut(s)	2297
BbsI	GAAGAC	3 cut(s)	1440, 2172, 2436
Bbv12I	GWGCWC	2 cut(s)	339, 1276
BbvI	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
BccI	CCATC	7 cut(s)	230, 387, 470, 715, 897, 1513, 2665
BceAI	ACGGC	5 cut(s)	110, 303, 367, 559, 1653
BciT130I	CCWGG	4 cut(s)	824, 1203, 1281, 1632
BciVI	GTATCC	1 cut(s)	896
BclI	TGATCA	1 cut(s)	931
BcnI	CCSGG	1 cut(s)	495
BcoDI	GTCTC	2 cut(s)	524, 612
BfaI	CTAG	3 cut(s)	615, 1790, 2721
BfoI	RGCGCY	2 cut(s)	1784, 2721
BfrI	CTTAAG	1 cut(s)	1655
BfuAI	ACCTGC	2 cut(s)	943, 1208
BfuI	GTATCC	1 cut(s)	896
Bme1390I	CCNGG	5 cut(s)	495, 824, 1203, 1281, 1632
Bme18I	GGWCC	4 cut(s)	955, 1133, 1465, 2557
BmgBI	CACGTC	1 cut(s)	2434
BmgT120I	GGNCC	5 cut(s)	955, 1067, 1133, 1465, 2557
BmiI	GGNNCC	4 cut(s)	1068, 1467, 1746, 2734
BmrFI	CCNGG	5 cut(s)	495, 824, 1203, 1281, 1632
BmrI	ACTGGG	4 cut(s)	808, 1683, 2057, 2183
BmsI	GCATC	5 cut(s)	796, 1718, 2263, 2578, 2782
BmuI	ACTGGG	4 cut(s)	808, 1683, 2057, 2183
BoxI	GACNNNNGTC	1 cut(s)	41
BpiI	GAAGAC	3 cut(s)	1440, 2172, 2436
BplI	GAGNNNNNCTC	4 cut(s)	80, 112, 180, 212
BpmI	CTGGAG	2 cut(s)	508, 767
Bpu14I	TTCGAA	1 cut(s)	425
BpuEI	CTTGAG	1 cut(s)	2196
BpuMI	CCSGG	1 cut(s)	495
BsaAI	YACGTR	2 cut(s)	2233, 2297
BsaBI	GATNNNNATC	2 cut(s)	222, 297
BsaHI	GRCGYC	3 cut(s)	23, 38, 354
BsaJI	CCNNGG	5 cut(s)	324, 1201, 1280, 2121, 2261
BsaXI	ACNNNNNCTCC	6 cut(s)	357, 387, 975, 1005, 1770, 1800
Bsc4I	CCNNNNNNNGG	5 cut(s)	178, 325, 1221, 1475, 1958
Bse118I	RCCGGY	1 cut(s)	417
Bse1I	ACTGG	7 cut(s)	247, 814, 1306, 1678, 1702, 2063, 2189
Bse21I	CCTNAGG	1 cut(s)	2626
Bse3DI	GCAATG	2 cut(s)	1872, 1935
Bse8I	GATNNNNATC	2 cut(s)	222, 297
BseBI	CCWGG	4 cut(s)	824, 1203, 1281, 1632
BseDI	CCNNGG	5 cut(s)	324, 1201, 1280, 2121, 2261
BseJI	GATNNNNATC	2 cut(s)	222, 297
BseLI	CCNNNNNNNGG	5 cut(s)	178, 325, 1221, 1475, 1958
BseMI	GCAATG	2 cut(s)	1872, 1935
BseMII	CTCAG	4 cut(s)	613, 1442, 2640, 2742
BseNI	ACTGG	7 cut(s)	247, 814, 1306, 1678, 1702, 2063, 2189
BseRI	GAGGAG	4 cut(s)	94, 194, 197, 1791
BseSI	GKGCMC	1 cut(s)	243
BseXI	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
BseYI	CCCAGC	1 cut(s)	1898
BsgI	GTGCAG	1 cut(s)	2513
Bsh1236I	CGCG	1 cut(s)	326
BshFI	GGCC	6 cut(s)	122, 421, 451, 1069, 1518, 1563
BsiHKAI	GWGCWC	2 cut(s)	339, 1276
BsiSI	CCGG	3 cut(s)	26, 418, 495
BslFI	GGGAC	1 cut(s)	1196
BslI	CCNNNNNNNGG	5 cut(s)	178, 325, 1221, 1475, 1958
BsmAI	GTCTC	2 cut(s)	524, 612
BsmBI	CGTCTC	1 cut(s)	612
BsmFI	GGGAC	1 cut(s)	1196
BsmI	GAATGC	3 cut(s)	486, 1124, 2406
BsnI	GGCC	6 cut(s)	122, 421, 451, 1069, 1518, 1563
Bsp119I	TTCGAA	1 cut(s)	425
Bsp1286I	GDGCHC	6 cut(s)	243, 339, 1202, 1276, 1725, 2634
BspANI	GGCC	6 cut(s)	122, 421, 451, 1069, 1518, 1563
BspCNI	CTCAG	4 cut(s)	612, 1441, 2639, 2741
BspFNI	CGCG	1 cut(s)	326
BspHI	TCATGA	1 cut(s)	1858
BspLI	GGNNCC	4 cut(s)	1068, 1467, 1746, 2734
BspMI	ACCTGC	2 cut(s)	943, 1208
BspQI	GCTCTTC	1 cut(s)	2748
BspT104I	TTCGAA	1 cut(s)	425
BspTI	CTTAAG	1 cut(s)	1655
BsrDI	GCAATG	2 cut(s)	1872, 1935
BsrFI	RCCGGY	1 cut(s)	417
BsrI	ACTGG	7 cut(s)	247, 814, 1306, 1678, 1702, 2063, 2189
BssAI	RCCGGY	1 cut(s)	417
BssECI	CCNNGG	5 cut(s)	324, 1201, 1280, 2121, 2261
BssNAI	GTATAC	1 cut(s)	1692
BssNI	GRCGYC	3 cut(s)	23, 38, 354
BssSI	CACGAG	2 cut(s)	73, 2579
BssT1I	CCWWGG	2 cut(s)	2121, 2261
Bst1107I	GTATAC	1 cut(s)	1692
Bst2BI	CACGAG	2 cut(s)	73, 2579
Bst2UI	CCWGG	4 cut(s)	824, 1203, 1281, 1632
Bst4CI	ACNGT	5 cut(s)	507, 1369, 1650, 2171, 2552
Bst6I	CTCTTC	7 cut(s)	112, 171, 203, 422, 1281, 1437, 2748
BstACI	GRCGYC	3 cut(s)	23, 38, 354
BstAFI	CTTAAG	1 cut(s)	1655
BstBAI	YACGTR	2 cut(s)	2233, 2297
BstBI	TTCGAA	1 cut(s)	425
BstC8I	GCNNGC	3 cut(s)	371, 1924, 2082
BstDEI	CTNAG	9 cut(s)	599, 1392, 1428, 1461, 1595, 1621, 2408, 2626, 2728
BstDSI	CCRYGG	1 cut(s)	324
BstENI	CCTNNNNNAGG	2 cut(s)	176, 1956
BstFNI	CGCG	1 cut(s)	326
BstH2I	RGCGCY	2 cut(s)	1784, 2721
BstHHI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
BstMAI	GTCTC	2 cut(s)	524, 612
BstMWI	GCNNNNNNNGC	3 cut(s)	31, 1119, 2126
BstNI	CCWGG	4 cut(s)	824, 1203, 1281, 1632
BstNSI	RCATGY	2 cut(s)	2396, 2420
BstPAI	GACNNNNGTC	1 cut(s)	41
BstSCI	CCNGG	5 cut(s)	493, 822, 1201, 1279, 1630
BstSLI	GKGCMC	1 cut(s)	243
BstSNI	TACGTA	1 cut(s)	2233
BstUI	CGCG	1 cut(s)	326
BstV1I	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
BstV2I	GAAGAC	3 cut(s)	1440, 2172, 2436
BstX2I	RGATCY	5 cut(s)	193, 1744, 2008, 2194, 2688
BstXI	CCANNNNNNTGG	2 cut(s)	799, 2486
BstYI	RGATCY	5 cut(s)	193, 1744, 2008, 2194, 2688
BstZ17I	GTATAC	1 cut(s)	1692
Bsu36I	CCTNAGG	1 cut(s)	2626
BsuI	GTATCC	1 cut(s)	896
BsuRI	GGCC	6 cut(s)	122, 421, 451, 1069, 1518, 1563
BtgI	CCRYGG	1 cut(s)	324
BtgZI	GCGATG	1 cut(s)	561
BtrI	CACGTC	1 cut(s)	2434
BtsIMutI	CAGTG	6 cut(s)	1147, 1299, 1365, 2067, 2176, 2557
BveI	ACCTGC	2 cut(s)	943, 1208
Cac8I	GCNNGC	3 cut(s)	371, 1924, 2082
CaiI	CAGNNNCTG	2 cut(s)	1733, 2069
CciI	TCATGA	1 cut(s)	1858
CfoI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
Cfr10I	RCCGGY	1 cut(s)	417
Cfr13I	GGNCC	5 cut(s)	955, 1067, 1133, 1465, 2557
Cfr42I	CCGCGG	1 cut(s)	327
CseI	GACGC	3 cut(s)	31, 343, 1008
Csp6I	GTAC	2 cut(s)	250, 2342
CspCI	CAANNNNNGTGG	4 cut(s)	1033, 1068, 1354, 1389
CviQI	GTAC	2 cut(s)	250, 2342
DdeI	CTNAG	9 cut(s)	599, 1392, 1428, 1461, 1595, 1621, 2408, 2626, 2728
EaeI	YGGCCR	1 cut(s)	1516
Eam1104I	CTCTTC	7 cut(s)	112, 171, 203, 422, 1281, 1437, 2748
EarI	CTCTTC	7 cut(s)	112, 171, 203, 422, 1281, 1437, 2748
EciI	GGCGGA	1 cut(s)	455
Ecl136II	GAGCTC	1 cut(s)	337
Eco105I	TACGTA	1 cut(s)	2233
Eco130I	CCWWGG	2 cut(s)	2121, 2261
Eco147I	AGGCCT	1 cut(s)	451
Eco24I	GRGCYC	4 cut(s)	339, 1202, 1725, 2634
Eco32I	GATATC	1 cut(s)	694
Eco47I	GGWCC	4 cut(s)	955, 1133, 1465, 2557
Eco47III	AGCGCT	2 cut(s)	1782, 2719
Eco53kI	GAGCTC	1 cut(s)	337
Eco57I	CTGAAG	2 cut(s)	2243, 2406
Eco72I	CACGTG	1 cut(s)	2297
Eco81I	CCTNAGG	1 cut(s)	2626
EcoICRI	GAGCTC	1 cut(s)	337
EcoNI	CCTNNNNNAGG	2 cut(s)	176, 1956
EcoO109I	RGGNCCY	1 cut(s)	1465
EcoRI	GAATTC	1 cut(s)	1081
EcoRII	CCWGG	4 cut(s)	822, 1201, 1279, 1630
EcoRV	GATATC	1 cut(s)	694
EcoT14I	CCWWGG	2 cut(s)	2121, 2261
EcoT38I	GRGCYC	4 cut(s)	339, 1202, 1725, 2634
ErhI	CCWWGG	2 cut(s)	2121, 2261
Esp3I	CGTCTC	1 cut(s)	612
FaqI	GGGAC	1 cut(s)	1196
FauI	CCCGC	1 cut(s)	1222
FauNDI	CATATG	1 cut(s)	2239
FbaI	TGATCA	1 cut(s)	931
FblI	GTMKAC	3 cut(s)	20, 129, 1691
FriOI	GRGCYC	4 cut(s)	339, 1202, 1725, 2634
FspBI	CTAG	3 cut(s)	615, 1790, 2721
GlaI	GCGC	5 cut(s)	707, 1503, 1782, 2676, 2719
GsaI	CCCAGC	1 cut(s)	1902
GsuI	CTGGAG	2 cut(s)	508, 767
HaeII	RGCGCY	2 cut(s)	1784, 2721
HaeIII	GGCC	6 cut(s)	122, 421, 451, 1069, 1518, 1563
HapII	CCGG	3 cut(s)	26, 418, 495
HgaI	GACGC	3 cut(s)	31, 343, 1008
HhaI	GCGC	5 cut(s)	708, 1504, 1783, 2677, 2720
Hin1I	GRCGYC	3 cut(s)	23, 38, 354
Hin6I	GCGC	5 cut(s)	706, 1502, 1781, 2675, 2718
HinP1I	GCGC	5 cut(s)	706, 1502, 1781, 2675, 2718
HincII	GTYRAC	4 cut(s)	21, 130, 2004, 2059
HindII	GTYRAC	4 cut(s)	21, 130, 2004, 2059
HindIII	AAGCTT	1 cut(s)	1181
HpaI	GTTAAC	1 cut(s)	2004
HpaII	CCGG	3 cut(s)	26, 418, 495
HphI	GGTGA	6 cut(s)	483, 658, 1637, 1861, 2626, 2768
Hpy99I	CGWCG	9 cut(s)	22, 25, 40, 116, 119, 131, 161, 164, 1002
HpyAV	CCTTC	5 cut(s)	432, 1766, 2533, 2570, 2716
HpyCH4III	ACNGT	5 cut(s)	507, 1369, 1650, 2171, 2552
HpyCH4IV	ACGT	5 cut(s)	38, 883, 2232, 2296, 2433
HpyF10VI	GCNNNNNNNGC	3 cut(s)	31, 1119, 2126
HpyF3I	CTNAG	9 cut(s)	599, 1392, 1428, 1461, 1595, 1621, 2408, 2626, 2728
HpySE526I	ACGT	5 cut(s)	38, 883, 2232, 2296, 2433
Hsp92I	GRCGYC	3 cut(s)	23, 38, 354
HspAI	GCGC	5 cut(s)	706, 1502, 1781, 2675, 2718
Ksp22I	TGATCA	1 cut(s)	931
KspAI	GTTAAC	1 cut(s)	2004
KspI	CCGCGG	1 cut(s)	327
LguI	GCTCTTC	1 cut(s)	2748
LmnI	GCTCC	7 cut(s)	378, 814, 1706, 1778, 1870, 2126, 2732
Lsp1109I	GCAGC	7 cut(s)	51, 1036, 1742, 1885, 1913, 1930, 1952
LweI	GCATC	5 cut(s)	796, 1718, 2263, 2578, 2782
MaeI	CTAG	3 cut(s)	615, 1790, 2721
MaeII	ACGT	5 cut(s)	38, 883, 2232, 2296, 2433
MfeI	CAATTG	1 cut(s)	1019
MflI	RGATCY	5 cut(s)	193, 1744, 2008, 2194, 2688
MhlI	GDGCHC	6 cut(s)	243, 339, 1202, 1276, 1725, 2634
MlsI	TGGCCA	1 cut(s)	1518
MluNI	TGGCCA	1 cut(s)	1518
MlyI	GAGTC	3 cut(s)	263, 266, 1759
MmeI	TCCRAC	3 cut(s)	135, 1936, 2727
Mox20I	TGGCCA	1 cut(s)	1518
MscI	TGGCCA	1 cut(s)	1518
MseI	TTAA	5 cut(s)	714, 1656, 2003, 2046, 2249
MslI	CAYNNNNRTG	2 cut(s)	671, 2643
Msp20I	TGGCCA	1 cut(s)	1518
MspA1I	CMGCKG	4 cut(s)	64, 326, 1733, 1943
MspCI	CTTAAG	1 cut(s)	1655
MspI	CCGG	3 cut(s)	26, 418, 495
MspR9I	CCNGG	5 cut(s)	495, 824, 1203, 1281, 1632
MunI	CAATTG	1 cut(s)	1019
Mva1269I	GAATGC	3 cut(s)	486, 1124, 2406
MvaI	CCWGG	4 cut(s)	824, 1203, 1281, 1632
MvnI	CGCG	1 cut(s)	326
MwoI	GCNNNNNNNGC	3 cut(s)	31, 1119, 2126
NciI	CCSGG	1 cut(s)	495
NdeI	CATATG	1 cut(s)	2239
NlaIV	GGNNCC	4 cut(s)	1068, 1467, 1746, 2734
NmuCI	GTSAC	8 cut(s)	347, 507, 991, 1297, 1906, 2065, 2102, 2112
NspI	RCATGY	2 cut(s)	2396, 2420
NspV	TTCGAA	1 cut(s)	425
OliI	CACNNNNGTG	1 cut(s)	671
PagI	TCATGA	1 cut(s)	1858
PceI	AGGCCT	1 cut(s)	451
PciSI	GCTCTTC	1 cut(s)	2748
PcsI	WCGNNNNNNNCGW	2 cut(s)	123, 249
PctI	GAATGC	3 cut(s)	486, 1124, 2406
PfeI	GAWTC	9 cut(s)	10, 29, 937, 973, 1970, 2156, 2321, 2366, 2462
PflFI	GACNNNGTC	2 cut(s)	353, 2063
PfoI	TCCNGGA	1 cut(s)	822
PleI	GAGTC	3 cut(s)	262, 266, 1759
PmaCI	CACGTG	1 cut(s)	2297
PmlI	CACGTG	1 cut(s)	2297
PpsI	GAGTC	3 cut(s)	262, 266, 1759
Ppu21I	YACGTR	2 cut(s)	2233, 2297
PpuMI	RGGWCCY	1 cut(s)	1465
PshAI	GACNNNNGTC	1 cut(s)	41
PshBI	ATTAAT	1 cut(s)	714
PsiI	TTATAA	1 cut(s)	2535
Psp124BI	GAGCTC	1 cut(s)	339
Psp5II	RGGWCCY	1 cut(s)	1465
Psp6I	CCWGG	4 cut(s)	822, 1201, 1279, 1630
PspCI	CACGTG	1 cut(s)	2297
PspFI	CCCAGC	1 cut(s)	1898
PspGI	CCWGG	4 cut(s)	822, 1201, 1279, 1630
PspN4I	GGNNCC	4 cut(s)	1068, 1467, 1746, 2734
PspPI	GGNCC	5 cut(s)	955, 1067, 1133, 1465, 2557
PspPPI	RGGWCCY	1 cut(s)	1465
PstNI	CAGNNNCTG	2 cut(s)	1733, 2069
PsuI	RGATCY	5 cut(s)	193, 1744, 2008, 2194, 2688
PsyI	GACNNNGTC	2 cut(s)	353, 2063
PvuII	CAGCTG	3 cut(s)	64, 1733, 1943
RsaI	GTAC	2 cut(s)	251, 2343
RsaNI	GTAC	2 cut(s)	250, 2342
RseI	CAYNNNNRTG	2 cut(s)	671, 2643
SacI	GAGCTC	1 cut(s)	339
SacII	CCGCGG	1 cut(s)	327
SalI	GTCGAC	2 cut(s)	19, 128
SapI	GCTCTTC	1 cut(s)	2748
SaqAI	TTAA	5 cut(s)	714, 1656, 2003, 2046, 2249
Sau96I	GGNCC	5 cut(s)	955, 1067, 1133, 1465, 2557
SchI	GAGTC	3 cut(s)	263, 266, 1759
ScrFI	CCNGG	5 cut(s)	495, 824, 1203, 1281, 1632
SduI	GDGCHC	6 cut(s)	243, 339, 1202, 1276, 1725, 2634
SfaNI	GCATC	5 cut(s)	796, 1718, 2263, 2578, 2782
Sfr303I	CCGCGG	1 cut(s)	327
SfuI	TTCGAA	1 cut(s)	425
SgrBI	CCGCGG	1 cut(s)	327
SgrDI	CGTCGACG	1 cut(s)	19
SinI	GGWCC	4 cut(s)	955, 1133, 1465, 2557
SmiMI	CAYNNNNRTG	2 cut(s)	671, 2643
SmlI	CTYRAG	2 cut(s)	1655, 2211
SmoI	CTYRAG	2 cut(s)	1655, 2211
SnaBI	TACGTA	1 cut(s)	2233
SseBI	AGGCCT	1 cut(s)	451
SspI	AATATT	2 cut(s)	787, 1150
SspMI	CTAG	3 cut(s)	615, 1790, 2721
SstI	GAGCTC	1 cut(s)	339
StuI	AGGCCT	1 cut(s)	451
StyD4I	CCNGG	5 cut(s)	493, 822, 1201, 1279, 1630
StyI	CCWWGG	2 cut(s)	2121, 2261
TaaI	ACNGT	5 cut(s)	507, 1369, 1650, 2171, 2552
TaiI	ACGT	5 cut(s)	41, 886, 2235, 2299, 2436
TaqI	TCGA	7 cut(s)	20, 129, 333, 339, 425, 696, 2460
TauI	GCSGC	3 cut(s)	81, 125, 326
TfiI	GAWTC	9 cut(s)	10, 29, 937, 973, 1970, 2156, 2321, 2366, 2462
Tru1I	TTAA	5 cut(s)	714, 1656, 2003, 2046, 2249
Tru9I	TTAA	5 cut(s)	714, 1656, 2003, 2046, 2249
TscAI	CASTG	6 cut(s)	1147, 1306, 1372, 2074, 2176, 2557
TseFI	GTSAC	8 cut(s)	347, 507, 991, 1297, 1906, 2065, 2102, 2112
TseI	GCWGC	7 cut(s)	64, 1024, 1730, 1873, 1926, 1940, 1943
Tsp45I	GTSAC	8 cut(s)	347, 507, 991, 1297, 1906, 2065, 2102, 2112
TspGWI	ACGGA	4 cut(s)	6, 71, 265, 2561
TspRI	CASTG	6 cut(s)	1147, 1306, 1372, 2074, 2176, 2557
Tth111I	GACNNNGTC	2 cut(s)	353, 2063
Vha464I	CTTAAG	1 cut(s)	1655
VpaK11BI	GGWCC	4 cut(s)	955, 1133, 1465, 2557
VspI	ATTAAT	1 cut(s)	714
XagI	CCTNNNNNAGG	2 cut(s)	176, 1956
XapI	RAATTY	6 cut(s)	569, 647, 1081, 1820, 2483, 2513
XceI	RCATGY	2 cut(s)	2396, 2420
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1199, 2743
XmiI	GTMKAC	3 cut(s)	20, 129, 1691
XspI	CTAG	3 cut(s)	615, 1790, 2721
ZraI	GACGTC	1 cut(s)	39

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.