AT1G47056

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012273
Physical Location & Seq
Reverse (-)
17275890 .. 17277839
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G47056.1

Sequence Viewer

Length: 1557 bp
ATGGGCCAATCAACCTCCGCCGCCGGAAATTCCATCCTCAATCGACGACGAAGCAAATCGTTTACACTGAAATTCCCGATTGAATCAATCGAATCTGAGATTTCACAACCTGACTACACATCGAGTTTACCTGACGAGTGCTTAGCTTTGGTGTTCCAGTTCTTAAACTCCGGTAATCGGAAAAGATGTGCTCTGGTTTGTCGTCGGTGGATGATCGTCGAAGGACAGAATCGTTACCGTCTTTCTCTCCACGCTCGCTCAGATCTGATTACTTCGATTCCTTCTCTCTTCTCTCGATTCGATTCCGTTACTAAGCTTTCGTTGAAATGCGATCGTAGATCTGTTAGTATCGGCGACGAAGCGCTTGTTAAGATCTCTCTCCGGTGTAGGAATTTGAAGAGACTTAAGCTTAGAGCTTGCCGTGAGCTCACCGATGTAGGTATGGCTGCTTTTGCTGAGAATTGTAAGGATTTGAAGATATTCTCTTGTGGTTCTTGTGATTTTGGAGCTAAAGGTGTGAAAGCTGTGCTTGATCATTGCTCGAATCTCGAGGAGTTATCGATTAAGCGTCTCCGTGGATTCACCGATATAGCTCCGGAGATGATTGGTCCTGGTGTTGCTGCTTCGTCTCTTAAGTCGATTTGCTTGAAGGAGCTTTATAATGGTCAGTGTTTCGGTCCTGTGATCGTTGGTGCGAAGAATCTTAAGTCTCTGAAGCTTTTTAGGTGTTCTGGTGATTGGGACTTGCTTCTTCAAGAGATGTCTGGTAAGGATCATGGTGTAGTGGAGATACATTTGGAACGTATGCAAGTTAGTGATGTAGCTTTATCTGCGATATCGTATTGCTCGAGTCTCGAGAGTTTGCATCTTGTGAAGACACCTGAATGCACGAATTTCGGATTAGCTGCGATTGCAGAGAAGTGCAAGCGTTTGAGGAAGCTTCATATTGATGGATGGAAAGCGAATTTGATTGGTGATGAAGGGCTTGTGGCTGTGGCTAAGTTTTGTTCTCAGTTACAGGAACTGGTACTGATTGGAGTGAATCCAACGACACTTAGTTTAGGAATGTTGGCTGCGAAATGTTTGAATCTTGAAAGATTGGCACTTTGTGGTTGTGATACGTTTGGTGATCCTGAGCTTTCTTGTATCGCAGCGAAATGTCCGGCTTTAAGGAAATTGTGTATCAAGAACTGTCCGATTTCGGATGTGGGAATCGAGAATCTTGCAAACGGATGTCCTGGTTTAACCAAAGTGAAAATCAAGAAGTGCAAAGGAGTATTGGGAGGGTGCGCAGACTGGTTAAGAACAGTTAGGCCTATGCTTTCGGTGAACGCAGACACCATGGAACAAGAACATGAAGAAGCAGCAAGCAATGATGTTGTAGGAGGATCGCAAGAAAACGGAATCGAATTCCCTCAATTGAACAGTCAGATCATGGCATCAAGCATTGCATCAAGTAGTAGAAACCGGTCAGGGTATTTCAAATCAGGCATTGGATTGTTCAGCGGAATGAGTCTAGTGCCTTGCACTTCGAGACAACGACGGGCAAGCAGGTGA

Protein Analysis

518

Amino Acids

56.5

Weight (kDa)

8.93

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 41 - 70 8.7e-07 F-box-like
F-box PF00646 41 - 71 3.3e-06 F-box domain
DUF7885 PF25372 279 - 426 3.5e-10 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370 PF24758 338 - 426 4.4e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47056 AT4G07400
fragaria_vesca FvH4_2g33740
malus_domestica MD08G1093600.v1.1 MD15G1077300.v1.1
prunus_persica Prupe.1G430700_v2.0.a1
pyrus_communis pycom08g07610 pycom12g16540 pycom15g07200
rosa_chinensis RchiOBHm_Chr6g0308811
rosa_laevigata RLG00000010616
rosa_multiflora Rmu_sc0004046.1_g000006
rosa_roxburghii Rroxscaffold_7G00159900
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479700 Rh6BG489300 Rh6CG494800 Rh6DG480000
rosa_wichuraiana Rw6G041820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 660
AarI CACCTGC 1 cut(s) 1542
Acc16I TGCGCA 1 cut(s) 1291
Acc36I ACCTGC 1 cut(s) 1542
AccIII TCCGGA 1 cut(s) 595
AciI CCGC 3 cut(s) 18, 21, 1506
AclWI GGATC 3 cut(s) 780, 1124, 1396
AcsI RAATTY 6 cut(s) 28, 71, 391, 892, 964, 1409
AcuI CTGAAG 1 cut(s) 734
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 1 cut(s) 1029
AfeI AGCGCT 1 cut(s) 363
AfiI CCNNNNNNNGG 1 cut(s) 177
AflII CTTAAG 3 cut(s) 404, 632, 704
AgeI ACCGGT 1 cut(s) 1467
AjnI CCWGG 2 cut(s) 610, 1237
Alw21I GWGCWC 2 cut(s) 193, 429
Alw26I GTCTC 6 cut(s) 394, 575, 633, 714, 857, 1528
AlwI GGATC 3 cut(s) 780, 1124, 1396
AlwNI CAGNNNCTG 1 cut(s) 1024
Ama87I CYCGRG 3 cut(s) 548, 847, 854
Aor13HI TCCGGA 1 cut(s) 595
Aor51HI AGCGCT 1 cut(s) 363
AoxI GGCC 2 cut(s) 4, 1313
ApeKI GCWGC 6 cut(s) 446, 620, 905, 1073, 1151, 1364
ApoI RAATTY 6 cut(s) 28, 71, 391, 892, 964, 1409
AsiGI ACCGGT 1 cut(s) 1467
Asp700I GAANNNNTTC 1 cut(s) 479
AspLEI GCGC 2 cut(s) 364, 1292
AspS9I GGNCC 3 cut(s) 4, 608, 677
AsuHPI GGTGA 6 cut(s) 421, 574, 746, 986, 1139, 1339
AvaI CYCGRG 3 cut(s) 548, 847, 854
AvaII GGWCC 2 cut(s) 608, 677
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 881
Bbv12I GWGCWC 2 cut(s) 193, 429
BbvI GCAGC 6 cut(s) 433, 607, 892, 1060, 1163, 1376
BccI CCATC 3 cut(s) 41, 944, 948
BceAI ACGGC 1 cut(s) 405
BcgI CGANNNNNNTGC 4 cut(s) 877, 911, 1205, 1239
BciT130I CCWGG 2 cut(s) 612, 1239
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 6 cut(s) 394, 575, 633, 714, 857, 1528
BfaI CTAG 1 cut(s) 1517
BfoI RGCGCY 1 cut(s) 365
BfrI CTTAAG 3 cut(s) 404, 632, 704
BfuAI ACCTGC 1 cut(s) 1542
BglII AGATCT 3 cut(s) 262, 338, 372
BisI GCNGC 7 cut(s) 21, 447, 621, 906, 1074, 1152, 1365
BlpI GCTNAGC 1 cut(s) 142
BlsI GCNGC 7 cut(s) 22, 448, 622, 907, 1075, 1153, 1366
Bme1390I CCNGG 2 cut(s) 612, 1239
Bme18I GGWCC 2 cut(s) 608, 677
BmeT110I CYCGRG 3 cut(s) 548, 847, 854
BmgT120I GGNCC 3 cut(s) 4, 608, 677
BmrFI CCNGG 2 cut(s) 612, 1239
BmsI GCATC 3 cut(s) 874, 1448, 1460
BpiI GAAGAC 1 cut(s) 881
Bpu10I CCTNAGC 1 cut(s) 1134
Bpu1102I GCTNAGC 1 cut(s) 142
Bsa29I ATCGAT 1 cut(s) 560
BsaJI CCNNGG 2 cut(s) 574, 1341
BsaWI WCCGGW 4 cut(s) 170, 381, 595, 1467
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse118I RCCGGY 1 cut(s) 1467
Bse1I ACTGG 3 cut(s) 157, 1029, 1301
Bse3DI GCAATG 3 cut(s) 535, 1378, 1446
BseAI TCCGGA 1 cut(s) 595
BseBI CCWGG 2 cut(s) 612, 1239
BseCI ATCGAT 1 cut(s) 560
BseDI CCNNGG 2 cut(s) 574, 1341
BseGI GGATG 5 cut(s) 33, 216, 959, 1210, 1238
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMI GCAATG 3 cut(s) 535, 1378, 1446
BseMII CTCAG 5 cut(s) 87, 273, 447, 1025, 1125
BseNI ACTGG 3 cut(s) 157, 1029, 1301
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 6 cut(s) 433, 607, 892, 1060, 1163, 1376
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 2 cut(s) 6, 1315
BshTI ACCGGT 1 cut(s) 1467
BshVI ATCGAT 1 cut(s) 560
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 2 cut(s) 193, 429
BsiHKCI CYCGRG 3 cut(s) 548, 847, 854
BsiSI CCGG 6 cut(s) 24, 171, 382, 596, 1163, 1468
BslFI GGGAC 1 cut(s) 755
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 6 cut(s) 394, 575, 633, 714, 857, 1528
BsmBI CGTCTC 2 cut(s) 575, 633
BsmFI GGGAC 1 cut(s) 755
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 2 cut(s) 6, 1315
BsoBI CYCGRG 3 cut(s) 548, 847, 854
Bsp1286I GDGCHC 2 cut(s) 193, 429
Bsp13I TCCGGA 1 cut(s) 595
Bsp1720I GCTNAGC 1 cut(s) 142
Bsp19I CCATGG 1 cut(s) 1341
BspACI CCGC 3 cut(s) 18, 21, 1506
BspANI GGCC 2 cut(s) 6, 1315
BspCNI CTCAG 5 cut(s) 88, 272, 448, 1024, 1126
BspDI ATCGAT 1 cut(s) 560
BspEI TCCGGA 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 1542
BspPI GGATC 3 cut(s) 780, 1124, 1396
BspTI CTTAAG 3 cut(s) 404, 632, 704
BsrDI GCAATG 3 cut(s) 535, 1378, 1446
BsrFI RCCGGY 1 cut(s) 1467
BsrI ACTGG 3 cut(s) 157, 1029, 1301
BssAI RCCGGY 1 cut(s) 1467
BssECI CCNNGG 2 cut(s) 574, 1341
BssT1I CCWWGG 1 cut(s) 1341
Bst2UI CCWGG 2 cut(s) 612, 1239
Bst4CI ACNGT 4 cut(s) 239, 1193, 1309, 1427
Bst6I CTCTTC 2 cut(s) 293, 392
BstAFI CTTAAG 3 cut(s) 404, 632, 704
BstC8I GCNNGC 5 cut(s) 256, 418, 926, 1369, 1549
BstDSI CCRYGG 2 cut(s) 574, 1341
BstF5I GGATG 5 cut(s) 33, 216, 959, 1210, 1238
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 2 cut(s) 364, 1292
BstMAI GTCTC 6 cut(s) 394, 575, 633, 714, 857, 1528
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 3 cut(s) 452, 830, 911
BstNI CCWGG 2 cut(s) 612, 1239
BstSCI CCNGG 2 cut(s) 610, 1237
BstV1I GCAGC 6 cut(s) 433, 607, 892, 1060, 1163, 1376
BstV2I GAAGAC 1 cut(s) 881
BstX2I RGATCY 3 cut(s) 262, 338, 372
BstYI RGATCY 3 cut(s) 262, 338, 372
Bsu15I ATCGAT 1 cut(s) 560
BsuRI GGCC 2 cut(s) 6, 1315
BsuTUI ATCGAT 1 cut(s) 560
BtgI CCRYGG 2 cut(s) 574, 1341
BtsCI GGATG 5 cut(s) 33, 216, 959, 1210, 1238
BtsIMutI CAGTG 2 cut(s) 65, 674
BveI ACCTGC 1 cut(s) 1542
Cac8I GCNNGC 5 cut(s) 256, 418, 926, 1369, 1549
CaiI CAGNNNCTG 1 cut(s) 1024
CfoI GCGC 2 cut(s) 364, 1292
Cfr10I RCCGGY 1 cut(s) 1467
Cfr13I GGNCC 3 cut(s) 4, 608, 677
ClaI ATCGAT 1 cut(s) 560
CseI GACGC 1 cut(s) 557
Csp6I GTAC 1 cut(s) 1028
CspAI ACCGGT 1 cut(s) 1467
CviAII CATG 4 cut(s) 776, 1342, 1355, 1435
CviQI GTAC 1 cut(s) 1028
DraIII CACNNNGTG 1 cut(s) 1109
Eam1104I CTCTTC 2 cut(s) 293, 392
EarI CTCTTC 2 cut(s) 293, 392
EciI GGCGGA 1 cut(s) 7
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 1341
Eco147I AGGCCT 1 cut(s) 1315
Eco24I GRGCYC 1 cut(s) 429
Eco32I GATATC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 608, 677
Eco47III AGCGCT 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 734
Eco88I CYCGRG 3 cut(s) 548, 847, 854
EcoICRI GAGCTC 1 cut(s) 427
EcoRI GAATTC 1 cut(s) 1409
EcoRII CCWGG 2 cut(s) 610, 1237
EcoRV GATATC 1 cut(s) 837
EcoT14I CCWWGG 1 cut(s) 1341
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 1341
Esp3I CGTCTC 2 cut(s) 575, 633
FaeI CATG 4 cut(s) 779, 1345, 1358, 1438
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FaqI GGGAC 1 cut(s) 755
FatI CATG 4 cut(s) 775, 1341, 1354, 1434
FbaI TGATCA 1 cut(s) 532
Fnu4HI GCNGC 7 cut(s) 21, 447, 621, 906, 1074, 1152, 1365
FokI GGATG 5 cut(s) 20, 223, 966, 1217, 1245
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 7 cut(s) 21, 447, 621, 906, 1074, 1152, 1365
FspBI CTAG 1 cut(s) 1517
FspI TGCGCA 1 cut(s) 1291
GlaI GCGC 2 cut(s) 363, 1291
GluI GCNGC 7 cut(s) 21, 447, 621, 906, 1074, 1152, 1365
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 2 cut(s) 6, 1315
HapII CCGG 6 cut(s) 24, 171, 382, 596, 1163, 1468
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 364, 1292
Hin1II CATG 4 cut(s) 779, 1345, 1358, 1438
Hin6I GCGC 2 cut(s) 362, 1290
HinP1I GCGC 2 cut(s) 362, 1290
HindIII AAGCTT 4 cut(s) 314, 407, 716, 938
HpaII CCGG 6 cut(s) 24, 171, 382, 596, 1163, 1468
HphI GGTGA 6 cut(s) 421, 574, 746, 986, 1139, 1339
Hpy166II GTNNAC 3 cut(s) 63, 128, 1330
Hpy188I TCNGA 9 cut(s) 97, 180, 262, 267, 714, 899, 1197, 1204, 1431
Hpy8I GTNNAC 3 cut(s) 63, 128, 1330
Hpy99I CGWCG 6 cut(s) 48, 51, 207, 221, 359, 1545
HpyAV CCTTC 4 cut(s) 215, 291, 643, 974
HpyCH4III ACNGT 4 cut(s) 239, 1193, 1309, 1427
HpyCH4IV ACGT 2 cut(s) 802, 1121
HpyCH4V TGCA 9 cut(s) 808, 865, 888, 914, 924, 1226, 1269, 1451, 1527
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 830, 911
HpySE526I ACGT 2 cut(s) 802, 1121
Hsp92II CATG 4 cut(s) 779, 1345, 1358, 1438
HspAI GCGC 2 cut(s) 362, 1290
Kpn2I TCCGGA 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 532
LmnI GCTCC 3 cut(s) 506, 598, 652
Lsp1109I GCAGC 6 cut(s) 433, 607, 892, 1060, 1163, 1376
LweI GCATC 3 cut(s) 874, 1448, 1460
MaeI CTAG 1 cut(s) 1517
MaeII ACGT 2 cut(s) 802, 1121
MaeIII GTNAC 3 cut(s) 233, 307, 1014
MboII GAAGA 7 cut(s) 280, 409, 487, 709, 743, 886, 1370
MfeI CAATTG 1 cut(s) 1418
MflI RGATCY 3 cut(s) 262, 338, 372
MhlI GDGCHC 2 cut(s) 193, 429
MluCI AATT 9 cut(s) 28, 71, 391, 460, 892, 964, 1175, 1409, 1418
MlyI GAGTC 2 cut(s) 859, 1522
MmeI TCCRAC 1 cut(s) 1070
MnlI CCTC 7 cut(s) 25, 47, 544, 927, 1277, 1379, 1425
MroI TCCGGA 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 9 cut(s) 164, 369, 405, 564, 633, 705, 1169, 1244, 1301
MslI CAYNNNNRTG 2 cut(s) 883, 948
MspA1I CMGCKG 1 cut(s) 1506
MspCI CTTAAG 3 cut(s) 404, 632, 704
MspI CCGG 6 cut(s) 24, 171, 382, 596, 1163, 1468
MspR9I CCNGG 2 cut(s) 612, 1239
MunI CAATTG 1 cut(s) 1418
Mva1269I GAATGC 1 cut(s) 890
MvaI CCWGG 2 cut(s) 612, 1239
MwoI GCNNNNNNNGC 3 cut(s) 452, 830, 911
NcoI CCATGG 1 cut(s) 1341
NlaIII CATG 4 cut(s) 779, 1345, 1358, 1438
NsbI TGCGCA 1 cut(s) 1291
PaeR7I CTCGAG 3 cut(s) 548, 847, 854
PaqCI CACCTGC 1 cut(s) 1542
PceI AGGCCT 1 cut(s) 1315
PcsI WCGNNNNNNNCGW 1 cut(s) 845
PctI GAATGC 1 cut(s) 890
PdmI GAANNNNTTC 1 cut(s) 479
PinAI ACCGGT 1 cut(s) 1467
PkrI GCNGC 7 cut(s) 22, 448, 622, 907, 1075, 1153, 1366
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 2 cut(s) 858, 1521
PpsI GAGTC 2 cut(s) 858, 1521
PsiI TTATAA 1 cut(s) 660
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 610, 1237
PspGI CCWGG 2 cut(s) 610, 1237
PspPI GGNCC 3 cut(s) 4, 608, 677
PspXI VCTCGAGB 1 cut(s) 847
PstNI CAGNNNCTG 1 cut(s) 1024
PsuI RGATCY 3 cut(s) 262, 338, 372
PvuI CGATCG 1 cut(s) 334
RsaI GTAC 1 cut(s) 1029
RsaNI GTAC 1 cut(s) 1028
RseI CAYNNNNRTG 2 cut(s) 883, 948
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 9 cut(s) 164, 369, 405, 564, 633, 705, 1169, 1244, 1301
SatI GCNGC 7 cut(s) 21, 447, 621, 906, 1074, 1152, 1365
Sau96I GGNCC 3 cut(s) 4, 608, 677
SchI GAGTC 2 cut(s) 859, 1522
ScrFI CCNGG 2 cut(s) 612, 1239
SduI GDGCHC 2 cut(s) 193, 429
SfaNI GCATC 3 cut(s) 874, 1448, 1460
Sfr274I CTCGAG 3 cut(s) 548, 847, 854
SinI GGWCC 2 cut(s) 608, 677
SlaI CTCGAG 3 cut(s) 548, 847, 854
SmiMI CAYNNNNRTG 2 cut(s) 883, 948
SmlI CTYRAG 6 cut(s) 404, 548, 632, 704, 847, 854
SmoI CTYRAG 6 cut(s) 404, 548, 632, 704, 847, 854
Sse9I AATT 9 cut(s) 28, 71, 391, 460, 892, 964, 1175, 1409, 1418
SseBI AGGCCT 1 cut(s) 1315
SsiI CCGC 3 cut(s) 18, 21, 1506
SspMI CTAG 1 cut(s) 1517
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 1315
StyD4I CCNGG 2 cut(s) 610, 1237
StyI CCWWGG 1 cut(s) 1341
TaaI ACNGT 4 cut(s) 239, 1193, 1309, 1427
TaiI ACGT 2 cut(s) 805, 1124
TaqII GACCGA 1 cut(s) 665
TasI AATT 9 cut(s) 28, 71, 391, 460, 892, 964, 1175, 1409, 1418
TauI GCSGC 1 cut(s) 23
Tru1I TTAA 9 cut(s) 164, 369, 405, 564, 633, 705, 1169, 1244, 1301
Tru9I TTAA 9 cut(s) 164, 369, 405, 564, 633, 705, 1169, 1244, 1301
TscAI CASTG 2 cut(s) 72, 674
TseI GCWGC 6 cut(s) 446, 620, 905, 1073, 1151, 1364
TspDTI ATGAA 3 cut(s) 932, 993, 1371
TspGWI ACGGA 4 cut(s) 295, 563, 1245, 1416
TspRI CASTG 2 cut(s) 72, 674
Vha464I CTTAAG 3 cut(s) 404, 632, 704
VpaK11BI GGWCC 2 cut(s) 608, 677
XapI RAATTY 6 cut(s) 28, 71, 391, 892, 964, 1409
XhoI CTCGAG 3 cut(s) 548, 847, 854
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 1 cut(s) 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.