Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012273

Physical Location & Seq

Genomic Coordinates Forward (+)

5283394 .. 5284995

Gene Structure 1602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1077300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1602 bp

ATGGGCCAGTCATCCTCGGCGGCCGCAAATTCAAGCCGCCGTGACAGCAACAACCACAGCCATCGATCCAAGTCCAAAGCAACGGCCGCGATCTCCCCGATGCTGCAGCCCGACGAGGAACGTTCTGAGATCGTCGACGTGGCGTCCGATTTCATCTCCGATCTTCCCGACGAGTGCTTGGCCTGCATATTCCACTCCCTCGGGTCCGGCGACCGGAAACGTTGCTCTCTGGTTTGCCGACGGTGGCTAAGGATCGAGGGACAGAACCGTCACCGTCTCTCCCTCAACGCCCAATCGGATCTCCTCCCCATCATCCCTTCCCTCTTCTCCCGCTTCGACGCCGTCACCAAGCTCGCCCTCAAATGCGACCGCAGATCCGTCAGCGTCGGCGACGAGGCGCTCGTCCTCATTTCTATGCGATGCCGTAACCTCACGCGCCTGAAGATCCGCGCGTGCCGCGAGTTGACCGATGCTGGGATGACGTCGCTCGCCAAAAACTGCAAAAGGTTGAAGAAGCTGTCGTGCGGGTCGTGCACGTTCGGAGCCAAAGGAATGAACGCAGTACTAGACAATTGCTCGGCGCTCGAAGAGTTATCTGTGAAGCGGCTCCGTGGCATCACCGACGGAGCGGCCTCGGAGCCGATCGGGCCTGGAGTGGCTGCCGCGTCGCTCAAAACGATTTGCCTGAAAGAACTCTACAATGGACAGTGCTTCGCGCCTTTGATAATGGGCGCGAAGAAACTGAGGACTCTGAAGCTCTTCAGGTGCACCGGCGATTGGGACAAACTTCTGCAAGTGATAGCCGAGCAAGTGACCTCCATGGTTGAGATCCACCTGGAAAAGCTTCAGGTCAGCGATGTAGGACTGGCCGCCATCTCCAATTGCTTGGATCTGGAGATTCTGCACCTGGTCAAGAGCCCCGAGTGGACCAATGTAGGATTGGTCTCCGTCGCCGAACGCTGCAAGCTGCTAAGGAAGCTTCACATCGACGGATGGAAGGCTAATCGAGTAGGCGACGAGGGTTTAGTCTCGGTGGCAAAGAACTGCTCTAACCTTCAGGAATTGGTGCTAATCGGTGTGAATCCGACCAAAGTGAGCTTGGAGGCATTGGCTTCCAATTGCCCCAATCTCGAAAGGTTGGCATTGTGCGAAAGCGTCACCGTGGGCGATGTGGAGATTTCGTCCATTGCGGTGAAGTGCGTGGCGTTGAAGAAGCTCTGCATAAAGAACTGCCCAGTCTCGGACATCGGCCTCGAAGCATTGGCCAGTGGTTGCCCTAACATGGTGAAAGTGAAGCTCAAGAAATGCAGAGGAGTAACAGCACAAGGAGCTGATTGGTTGAGGGCAAGTCGGCCTTTGCTGGCTGTGAATTTAGATACAGGTGAAACAATCCAAGAGGCCGCCATCGATAACTCCGATGTGGCCCACAATAACTCCGTCGAGTTCCCGCCGATGGCCATCCAACCGGCGTTTCCGGCCATTGCCTCAAGCAGTGCAGGGCGGTCGACGTCGTTTAAGTCGAGGTTAGGACTTTGGACAGGGAGGAGGGGTTTTGTGGCTTGCACTCTGAGAAGGTGGTCGTCAAGTCGGAATAATACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like LRR_6

Protein Analysis

534

Amino Acids

57.72

Weight (kDa)

9.11

Isoelectric Point (pI)

47.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box-like	PF12937	52 - 85	7.5e-08	F-box-like
F-box	PF00646	52 - 85	1.3e-07	F-box domain
DUF7885	PF25372	283 - 445	1.3e-10	Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370	PF24758	298 - 409	3.8e-10	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012273)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47056 AT4G07400
fragaria_vesca	FvH4_2g33740
malus_domestica	MD08G1093600.v1.1 MD15G1077300.v1.1
prunus_persica	Prupe.1G430700_v2.0.a1
pyrus_communis	pycom08g07610 pycom12g16540 pycom15g07200
rosa_chinensis	RchiOBHm_Chr6g0308811
rosa_laevigata	RLG00000010616
rosa_multiflora	Rmu_sc0004046.1_g000006
rosa_roxburghii	Rroxscaffold_7G00159900
rosa_rugosa	Rorug06G0368300
rosa_samantha	Rh6AG479700 Rh6BG489300 Rh6CG494800 Rh6DG480000
rosa_wichuraiana	Rw6G041820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	267
AatII	GACGTC	2 cut(s)	485, 1511
AccBSI	CCGCTC	1 cut(s)	629
AccI	GTMKAC	2 cut(s)	135, 1505
AccII	CGCG	8 cut(s)	89, 436, 450, 452, 459, 665, 716, 734
AclI	AACGTT	2 cut(s)	121, 220
AclWI	GGATC	7 cut(s)	60, 260, 306, 369, 439, 823, 897
AcoI	YGGCCR	6 cut(s)	21, 84, 867, 1263, 1455, 1476
AcsI	RAATTY	2 cut(s)	28, 1369
AcuI	CTGAAG	5 cut(s)	461, 745, 773, 830, 1040
AcyI	GRCGYC	4 cut(s)	143, 339, 482, 1508
AfaI	GTAC	1 cut(s)	564
AfiI	CCNNNNNNNGG	6 cut(s)	213, 474, 777, 1240, 1282, 1453
AgsI	TTSAA	3 cut(s)	33, 511, 1210
AhdI	GACNNNNNGTC	1 cut(s)	142
AjiI	CACGTC	1 cut(s)	139
AjnI	CCWGG	3 cut(s)	649, 834, 906
AjuI	GAANNNNNNNTTGG	2 cut(s)	1455, 1487
Alw21I	GWGCWC	2 cut(s)	536, 770
Alw26I	GTCTC	4 cut(s)	281, 949, 1033, 1243
Alw44I	GTGCAC	2 cut(s)	532, 766
AlwI	GGATC	7 cut(s)	60, 260, 306, 369, 439, 823, 897
Ama87I	CYCGRG	2 cut(s)	200, 920
ApaLI	GTGCAC	2 cut(s)	532, 766
ApeKI	GCWGC	5 cut(s)	103, 106, 659, 960, 967
ApoI	RAATTY	2 cut(s)	28, 1369
Asp700I	GAANNNNTTC	2 cut(s)	758, 843
AspLEI	GCGC	6 cut(s)	400, 438, 452, 583, 718, 734
AspS9I	GGNCC	5 cut(s)	4, 204, 647, 927, 1423
AsuHPI	GGTGA	7 cut(s)	263, 337, 610, 1150, 1204, 1297, 1394
AvaI	CYCGRG	2 cut(s)	200, 920
AvaII	GGWCC	2 cut(s)	204, 927
BaeGI	GKGCMC	2 cut(s)	536, 770
BalI	TGGCCA	2 cut(s)	1265, 1457
BanII	GRGCYC	1 cut(s)	920
Bbv12I	GWGCWC	2 cut(s)	536, 770
BbvI	GCAGC	5 cut(s)	90, 118, 646, 947, 954
BccI	CCATC	7 cut(s)	69, 317, 881, 987, 1412, 1447, 1466
BceAI	ACGGC	4 cut(s)	24, 99, 326, 408
BcgI	CGANNNNNNTGC	2 cut(s)	1485, 1519
BciT130I	CCWGG	3 cut(s)	651, 836, 908
BcoDI	GTCTC	4 cut(s)	281, 949, 1033, 1243
BfaI	CTAG	2 cut(s)	566, 1600
BfmI	CTRYAG	1 cut(s)	104
BfoI	RGCGCY	2 cut(s)	401, 584
BglI	GCCNNNNNGGC	1 cut(s)	646
BmcAI	AGTACT	1 cut(s)	564
Bme1390I	CCNGG	3 cut(s)	651, 836, 908
Bme18I	GGWCC	2 cut(s)	204, 927
BmeRI	GACNNNNNGTC	1 cut(s)	142
BmeT110I	CYCGRG	2 cut(s)	200, 920
BmgBI	CACGTC	1 cut(s)	139
BmgT120I	GGNCC	5 cut(s)	4, 204, 647, 927, 1423
BmiI	GGNNCC	4 cut(s)	205, 544, 608, 639
BmrFI	CCNGG	3 cut(s)	651, 836, 908
BmrI	ACTGGG	1 cut(s)	1229
BmsI	GCATC	4 cut(s)	90, 410, 460, 624
BmuI	ACTGGG	1 cut(s)	1229
BpmI	CTGGAG	2 cut(s)	672, 914
Bpu10I	CCTNAGC	2 cut(s)	248, 971
BpuEI	CTTGAG	2 cut(s)	1283, 1471
Bsa29I	ATCGAT	2 cut(s)	64, 1407
BsaBI	GATNNNNATC	1 cut(s)	1457
BsaHI	GRCGYC	4 cut(s)	143, 339, 482, 1508
BsaI	GGTCTC	1 cut(s)	949
BsaJI	CCNNGG	6 cut(s)	15, 199, 610, 633, 819, 1161
BsaWI	WCCGGW	1 cut(s)	213
BsaXI	ACNNNNNCTCC	8 cut(s)	263, 293, 1166, 1196, 1418, 1448, 1534, 1564
Bsc4I	CCNNNNNNNGG	6 cut(s)	213, 474, 777, 1240, 1282, 1453
Bse118I	RCCGGY	2 cut(s)	770, 1465
Bse1I	ACTGG	4 cut(s)	7, 870, 1235, 1266
Bse3DI	GCAATG	2 cut(s)	1185, 1479
Bse8I	GATNNNNATC	1 cut(s)	1457
BseBI	CCWGG	3 cut(s)	651, 836, 908
BseCI	ATCGAT	2 cut(s)	64, 1407
BseDI	CCNNGG	6 cut(s)	15, 199, 610, 633, 819, 1161
BseGI	GGATG	5 cut(s)	11, 312, 483, 998, 1458
BseJI	GATNNNNATC	1 cut(s)	1457
BseLI	CCNNNNNNNGG	6 cut(s)	213, 474, 777, 1240, 1282, 1453
BseMI	GCAATG	2 cut(s)	1185, 1479
BseMII	CTCAG	3 cut(s)	117, 734, 1559
BseNI	ACTGG	4 cut(s)	7, 870, 1235, 1266
BseRI	GAGGAG	3 cut(s)	293, 1326, 1558
BseSI	GKGCMC	2 cut(s)	536, 770
BseX3I	CGGCCG	2 cut(s)	21, 84
BseXI	GCAGC	5 cut(s)	90, 118, 646, 947, 954
BseYI	CCCAGC	1 cut(s)	473
BsgI	GTGCAG	2 cut(s)	887, 1515
Bsh1236I	CGCG	8 cut(s)	89, 436, 450, 452, 459, 665, 716, 734
Bsh1285I	CGRYCG	6 cut(s)	24, 87, 214, 370, 645, 1505
BshVI	ATCGAT	2 cut(s)	64, 1407
BsiEI	CGRYCG	6 cut(s)	24, 87, 214, 370, 645, 1505
BsiHKAI	GWGCWC	2 cut(s)	536, 770
BsiHKCI	CYCGRG	2 cut(s)	200, 920
BsiSI	CCGG	5 cut(s)	207, 214, 771, 1466, 1475
BslFI	GGGAC	2 cut(s)	273, 794
BslI	CCNNNNNNNGG	6 cut(s)	213, 474, 777, 1240, 1282, 1453
BsmAI	GTCTC	4 cut(s)	281, 949, 1033, 1243
BsmBI	CGTCTC	1 cut(s)	281
BsmFI	GGGAC	2 cut(s)	273, 794
Bso31I	GGTCTC	1 cut(s)	949
BsoBI	CYCGRG	2 cut(s)	200, 920
Bsp1286I	GDGCHC	3 cut(s)	536, 770, 920
Bsp19I	CCATGG	1 cut(s)	819
BspCNI	CTCAG	3 cut(s)	118, 735, 1560
BspDI	ATCGAT	2 cut(s)	64, 1407
BspFNI	CGCG	8 cut(s)	89, 436, 450, 452, 459, 665, 716, 734
BspLI	GGNNCC	4 cut(s)	205, 544, 608, 639
BspMAI	CTGCAG	1 cut(s)	108
BspPI	GGATC	7 cut(s)	60, 260, 306, 369, 439, 823, 897
BspQI	GCTCTTC	1 cut(s)	764
BspTNI	GGTCTC	1 cut(s)	949
BsrBI	CCGCTC	1 cut(s)	629
BsrDI	GCAATG	2 cut(s)	1185, 1479
BsrFI	RCCGGY	2 cut(s)	770, 1465
BsrI	ACTGG	4 cut(s)	7, 870, 1235, 1266
BssAI	RCCGGY	2 cut(s)	770, 1465
BssECI	CCNNGG	6 cut(s)	15, 199, 610, 633, 819, 1161
BssNI	GRCGYC	4 cut(s)	143, 339, 482, 1508
BssT1I	CCWWGG	1 cut(s)	819
Bst2UI	CCWGG	3 cut(s)	651, 836, 908
Bst4CI	ACNGT	5 cut(s)	243, 269, 275, 708, 1162
Bst6I	CTCTTC	3 cut(s)	329, 582, 764
BstACI	GRCGYC	4 cut(s)	143, 339, 482, 1508
BstC8I	GCNNGC	7 cut(s)	184, 354, 454, 489, 965, 1362, 1561
BstDEI	CTNAG	5 cut(s)	126, 248, 743, 971, 1568
BstDSI	CCRYGG	3 cut(s)	610, 819, 1161
BstF5I	GGATG	5 cut(s)	11, 312, 483, 998, 1458
BstFNI	CGCG	8 cut(s)	89, 436, 450, 452, 459, 665, 716, 734
BstH2I	RGCGCY	2 cut(s)	401, 584
BstHHI	GCGC	6 cut(s)	400, 438, 452, 583, 718, 734
BstMAI	GTCTC	4 cut(s)	281, 949, 1033, 1243
BstMCI	CGRYCG	6 cut(s)	24, 87, 214, 370, 645, 1505
BstMWI	GCNNNNNNNGC	9 cut(s)	45, 86, 183, 456, 531, 646, 976, 1328, 1475
BstNI	CCWGG	3 cut(s)	651, 836, 908
BstSCI	CCNGG	3 cut(s)	649, 834, 906
BstSFI	CTRYAG	1 cut(s)	104
BstSLI	GKGCMC	2 cut(s)	536, 770
BstUI	CGCG	8 cut(s)	89, 436, 450, 452, 459, 665, 716, 734
BstV1I	GCAGC	5 cut(s)	90, 118, 646, 947, 954
BstX2I	RGATCY	5 cut(s)	298, 374, 444, 828, 889
BstXI	CCANNNNNNTGG	1 cut(s)	886
BstYI	RGATCY	5 cut(s)	298, 374, 444, 828, 889
BstZI	CGGCCG	2 cut(s)	21, 84
Bsu15I	ATCGAT	2 cut(s)	64, 1407
BsuTUI	ATCGAT	2 cut(s)	64, 1407
BtgI	CCRYGG	3 cut(s)	610, 819, 1161
BtgZI	GCGATG	3 cut(s)	433, 870, 1182
BtrI	CACGTC	1 cut(s)	139
BtsCI	GGATG	5 cut(s)	11, 312, 483, 998, 1458
BtsI	GCAGTG	1 cut(s)	1498
BtsIMutI	CAGTG	3 cut(s)	713, 1273, 1498
Cac8I	GCNNGC	7 cut(s)	184, 354, 454, 489, 965, 1362, 1561
CciNI	GCGGCCGC	1 cut(s)	21
CfoI	GCGC	6 cut(s)	400, 438, 452, 583, 718, 734
Cfr10I	RCCGGY	2 cut(s)	770, 1465
Cfr13I	GGNCC	5 cut(s)	4, 204, 647, 927, 1423
ClaI	ATCGAT	2 cut(s)	64, 1407
CseI	GACGC	5 cut(s)	132, 347, 373, 654, 1144
CsiI	ACCWGGT	1 cut(s)	906
Csp6I	GTAC	1 cut(s)	563
CviAII	CATG	2 cut(s)	820, 1282
CviQI	GTAC	1 cut(s)	563
DdeI	CTNAG	5 cut(s)	126, 248, 743, 971, 1568
DrdI	GACNNNNNNGTC	1 cut(s)	267
DriI	GACNNNNNGTC	1 cut(s)	142
DseDI	GACNNNNNNGTC	1 cut(s)	267
EaeI	YGGCCR	6 cut(s)	21, 84, 867, 1263, 1455, 1476
EagI	CGGCCG	2 cut(s)	21, 84
Eam1104I	CTCTTC	3 cut(s)	329, 582, 764
Eam1105I	GACNNNNNGTC	1 cut(s)	142
EarI	CTCTTC	3 cut(s)	329, 582, 764
EclXI	CGGCCG	2 cut(s)	21, 84
Eco130I	CCWWGG	1 cut(s)	819
Eco24I	GRGCYC	1 cut(s)	920
Eco31I	GGTCTC	1 cut(s)	949
Eco47I	GGWCC	2 cut(s)	204, 927
Eco52I	CGGCCG	2 cut(s)	21, 84
Eco57I	CTGAAG	5 cut(s)	461, 745, 773, 830, 1040
Eco88I	CYCGRG	2 cut(s)	200, 920
EcoRII	CCWGG	3 cut(s)	649, 834, 906
EcoT14I	CCWWGG	1 cut(s)	819
EcoT38I	GRGCYC	1 cut(s)	920
ErhI	CCWWGG	1 cut(s)	819
Esp3I	CGTCTC	1 cut(s)	281
FaeI	CATG	2 cut(s)	823, 1285
FaiI	YATR	5 cut(s)	188, 416, 821, 1223, 1283
FalI	AAGNNNNNCTT	2 cut(s)	1339, 1371
FaqI	GGGAC	2 cut(s)	273, 794
FatI	CATG	2 cut(s)	819, 1281
FauI	CCCGC	3 cut(s)	338, 518, 1455
FblI	GTMKAC	2 cut(s)	135, 1505
FokI	GGATG	4 cut(s)	299, 490, 1005, 1445
FriOI	GRGCYC	1 cut(s)	920
FspBI	CTAG	2 cut(s)	566, 1600
GlaI	GCGC	6 cut(s)	399, 437, 451, 582, 717, 733
GsaI	CCCAGC	1 cut(s)	477
GsuI	CTGGAG	2 cut(s)	672, 914
HaeII	RGCGCY	2 cut(s)	401, 584
HapII	CCGG	5 cut(s)	207, 214, 771, 1466, 1475
HgaI	GACGC	5 cut(s)	132, 347, 373, 654, 1144
HhaI	GCGC	6 cut(s)	400, 438, 452, 583, 718, 734
Hin1I	GRCGYC	4 cut(s)	143, 339, 482, 1508
Hin1II	CATG	2 cut(s)	823, 1285
Hin6I	GCGC	6 cut(s)	398, 436, 450, 581, 716, 732
HinP1I	GCGC	6 cut(s)	398, 436, 450, 581, 716, 732
HincII	GTYRAC	3 cut(s)	136, 465, 1506
HindII	GTYRAC	3 cut(s)	136, 465, 1506
HindIII	AAGCTT	2 cut(s)	842, 977
HinfI	GANTC	3 cut(s)	748, 898, 1081
HpaII	CCGG	5 cut(s)	207, 214, 771, 1466, 1475
HphI	GGTGA	7 cut(s)	263, 337, 610, 1150, 1204, 1297, 1394
Hpy166II	GTNNAC	6 cut(s)	136, 465, 534, 768, 927, 1506
Hpy188III	TCNNGA	6 cut(s)	167, 893, 913, 1058, 1130, 1300
Hpy8I	GTNNAC	6 cut(s)	136, 465, 534, 768, 927, 1506
HpyAV	CCTTC	4 cut(s)	327, 991, 1064, 1566
HpyCH4III	ACNGT	5 cut(s)	243, 269, 275, 708, 1162
HpyCH4IV	ACGT	6 cut(s)	121, 138, 220, 482, 536, 1508
HpyF10VI	GCNNNNNNNGC	9 cut(s)	45, 86, 183, 456, 531, 646, 976, 1328, 1475
HpyF3I	CTNAG	5 cut(s)	126, 248, 743, 971, 1568
HpySE526I	ACGT	6 cut(s)	121, 138, 220, 482, 536, 1508
Hsp92I	GRCGYC	4 cut(s)	143, 339, 482, 1508
Hsp92II	CATG	2 cut(s)	823, 1285
HspAI	GCGC	6 cut(s)	398, 436, 450, 581, 716, 732
LguI	GCTCTTC	1 cut(s)	764
LmnI	GCTCC	5 cut(s)	542, 612, 626, 637, 1328
Lsp1109I	GCAGC	5 cut(s)	90, 118, 646, 947, 954
LweI	GCATC	4 cut(s)	90, 410, 460, 624
MabI	ACCWGGT	1 cut(s)	906
MaeI	CTAG	2 cut(s)	566, 1600
MaeII	ACGT	6 cut(s)	121, 138, 220, 482, 536, 1508
MaeIII	GTNAC	7 cut(s)	41, 269, 343, 425, 811, 1156, 1315
MbiI	CCGCTC	1 cut(s)	629
MboII	GAAGA	8 cut(s)	155, 316, 454, 523, 599, 748, 751, 1222
MfeI	CAATTG	3 cut(s)	571, 880, 1117
MflI	RGATCY	5 cut(s)	298, 374, 444, 828, 889
MhlI	GDGCHC	3 cut(s)	536, 770, 920
MlsI	TGGCCA	2 cut(s)	1265, 1457
MluCI	AATT	6 cut(s)	28, 571, 880, 1061, 1117, 1369
MluNI	TGGCCA	2 cut(s)	1265, 1457
MlyI	GAGTC	1 cut(s)	742
MmeI	TCCRAC	3 cut(s)	1109, 1486, 1568
Mox20I	TGGCCA	2 cut(s)	1265, 1457
MroXI	GAANNNNTTC	2 cut(s)	758, 843
MscI	TGGCCA	2 cut(s)	1265, 1457
MseI	TTAA	1 cut(s)	1515
MslI	CAYNNNNRTG	2 cut(s)	413, 1190
Msp20I	TGGCCA	2 cut(s)	1265, 1457
MspI	CCGG	5 cut(s)	207, 214, 771, 1466, 1475
MspR9I	CCNGG	3 cut(s)	651, 836, 908
MunI	CAATTG	3 cut(s)	571, 880, 1117
MvaI	CCWGG	3 cut(s)	651, 836, 908
MvnI	CGCG	8 cut(s)	89, 436, 450, 452, 459, 665, 716, 734
MwoI	GCNNNNNNNGC	9 cut(s)	45, 86, 183, 456, 531, 646, 976, 1328, 1475
NcoI	CCATGG	1 cut(s)	819
NlaIII	CATG	2 cut(s)	823, 1285
NlaIV	GGNNCC	4 cut(s)	205, 544, 608, 639
NmeAIII	GCCGAG	2 cut(s)	557, 829
NmuCI	GTSAC	5 cut(s)	41, 269, 343, 811, 1156
NotI	GCGGCCGC	1 cut(s)	21
PciSI	GCTCTTC	1 cut(s)	764
PcsI	WCGNNNNNNNCGW	7 cut(s)	144, 207, 399, 527, 674, 1413, 1517
PdmI	GAANNNNTTC	2 cut(s)	758, 843
PfeI	GAWTC	2 cut(s)	898, 1081
PflFI	GACNNNGTC	1 cut(s)	341
Ple19I	CGATCG	1 cut(s)	645
PleI	GAGTC	1 cut(s)	742
PpsI	GAGTC	1 cut(s)	742
Psp1406I	AACGTT	2 cut(s)	121, 220
Psp6I	CCWGG	3 cut(s)	649, 834, 906
PspFI	CCCAGC	1 cut(s)	473
PspGI	CCWGG	3 cut(s)	649, 834, 906
PspN4I	GGNNCC	4 cut(s)	205, 544, 608, 639
PspPI	GGNCC	5 cut(s)	4, 204, 647, 927, 1423
PstI	CTGCAG	1 cut(s)	108
PsuI	RGATCY	5 cut(s)	298, 374, 444, 828, 889
PsyI	GACNNNGTC	1 cut(s)	341
PvuI	CGATCG	1 cut(s)	645
RsaI	GTAC	1 cut(s)	564
RsaNI	GTAC	1 cut(s)	563
RseI	CAYNNNNRTG	2 cut(s)	413, 1190
SalI	GTCGAC	2 cut(s)	134, 1504
SapI	GCTCTTC	1 cut(s)	764
SaqAI	TTAA	1 cut(s)	1515
Sau96I	GGNCC	5 cut(s)	4, 204, 647, 927, 1423
ScaI	AGTACT	1 cut(s)	564
SchI	GAGTC	1 cut(s)	742
ScrFI	CCNGG	3 cut(s)	651, 836, 908
SduI	GDGCHC	3 cut(s)	536, 770, 920
SexAI	ACCWGGT	1 cut(s)	906
SfaNI	GCATC	4 cut(s)	90, 410, 460, 624
SfcI	CTRYAG	1 cut(s)	104
SgrAI	CRCCGGYG	1 cut(s)	770
SgrDI	CGTCGACG	1 cut(s)	134
SinI	GGWCC	2 cut(s)	204, 927
SmiMI	CAYNNNNRTG	2 cut(s)	413, 1190
SmlI	CTYRAG	2 cut(s)	1298, 1486
SmoI	CTYRAG	2 cut(s)	1298, 1486
Sse9I	AATT	6 cut(s)	28, 571, 880, 1061, 1117, 1369
SspMI	CTAG	2 cut(s)	566, 1600
StyD4I	CCNGG	3 cut(s)	649, 834, 906
StyI	CCWWGG	1 cut(s)	819
TaaI	ACNGT	5 cut(s)	243, 269, 275, 708, 1162
TaiI	ACGT	6 cut(s)	124, 141, 223, 485, 539, 1511
TaqII	GACCGA	1 cut(s)	482
TasI	AATT	6 cut(s)	28, 571, 880, 1061, 1117, 1369
TatI	WGTACW	1 cut(s)	562
TfiI	GAWTC	2 cut(s)	898, 1081
Tru1I	TTAA	1 cut(s)	1515
Tru9I	TTAA	1 cut(s)	1515
TscAI	CASTG	3 cut(s)	713, 1273, 1498
TseFI	GTSAC	5 cut(s)	41, 269, 343, 811, 1156
TseI	GCWGC	5 cut(s)	103, 106, 659, 960, 967
Tsp45I	GTSAC	5 cut(s)	41, 269, 343, 811, 1156
TspDTI	ATGAA	2 cut(s)	142, 569
TspGWI	ACGGA	6 cut(s)	367, 599, 639, 937, 1005, 1426
TspRI	CASTG	3 cut(s)	713, 1273, 1498
Tth111I	GACNNNGTC	1 cut(s)	341
VneI	GTGCAC	2 cut(s)	532, 766
VpaK11BI	GGWCC	2 cut(s)	204, 927
XapI	RAATTY	2 cut(s)	28, 1369
XcmI	CCANNNNNNNNNTGG	2 cut(s)	937, 1096
XmiI	GTMKAC	2 cut(s)	135, 1505
XmnI	GAANNNNTTC	2 cut(s)	758, 843
XspI	CTAG	2 cut(s)	566, 1600
ZraI	GACGTC	2 cut(s)	483, 1509
ZrmI	AGTACT	1 cut(s)	564

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.