pycom15g07200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012273
Physical Location & Seq
Forward (+)
4416217 .. 4417818
1602 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g07200.1

Sequence Viewer

Length: 1602 bp
ATGGGCCAGTCATCCTCGGCGGCCGCAAATTCAAGCCGCCTTGACAGCAACAACCACAGCCATCGATCCAAGTCCAAAGCAACGGCCGCGATCTCCCCGATGCTGCAGGCCGACGAGGAACGTTCTGGGATCGTCGACGTGGCGTCCGATTTAATCTCCGATCTTCCCGACGAGTGTTTGGCCTGCATATTCCAGTCCCTCGGGTCCGGCGACCGGAAACGTTGCTCTCTGGTTTGCCGACGGTGGCTAAGGATCGAGGGACAGAACTGTCACCGTCTCTCCCTCAACGCCCAATCGGATCTCCTCCCCATCATCCCTTCCCTCTTCTCCCGCTTCGACGCCGTCACCAAGCTCGCCCTCAAATGCGACCGCAGATCCGTCAGCGTCGGCGACGAGGCACTCGTCCTCATTTCTATGCGATGCCGTAACCTCACGCGCCTGAAGATCCGCGCGTGCCGCGAGTTGACCGATGCTGGGATGATGTCGCTCGCCAAAAACTGCAAAAGGTTGAAGAAGCTGTCGTGCGGGTGGTGCACGTTCGGAGCCGAAGGAATGAACGCAGTACTAGACAATTGCTCGGCGCTCGAAGAGTTATCGGTGAAGCGGCTCCGTGGCAACACCGACGGAGCGGCCTCGGAGCCGATCGGGCCCGGAGTGGCTGCCGCGTCGCTCAAAACGATTTGCCTGAAAGAACTCTACAATGGACAGTGCTTCGCGCCCTTGATAATGGGCGCGAAGAAACTGAGGACTCTGAAGCTCTTCAGGTGCACCGGCGATTGGGACAAACTTCTGCAAGTGATAGCCGAGCAAGTGACCTCCATGGTTGAGATCCACCTGGAAAAGCTTCAGGTCAGCGATGTAGGACTGGCCGCCATCTCCAATTGCTTGGATCTGGAGATTCTGCACCTGGTCAAGAGCCCCGAGTGGACCAATGTAGGATTGGTCTCCGTCGCCGAACGCTGCAAGCTGCTAAGGAAGCTTCACATCGACGGATGGAAGGCTAATCGAGTAGGCGACGAGGGTTTAGTCTCGGTGGCAAAGAACTGCTCCAACCTTCAGGAATTGGTGCTAATCGGTGTGAATCCGACCAAAGTGAGCTTGGAGGCATTGGCTTCCAATTGCCCCAATCTCGAAAGGTTGGCATTGTGCGAAAGCATCACCGTGGGCGATGTGGAGATTTCGTGCATTGCGGTGAAGTGCGTGGCGTTGAAGAAGCTCTGCATAAAGAACTGCCCAGTCTCGAACATCGGCCTCGAGGCATTGGCCAGTGGTTGCCCTAACATGGTGAAAGTGAAGCTCAAGAAATGCAGAGGAGTAACAGCAGAAGGAGCTGATTGGTTGAGGGCAAGTCGGCCTTTGCTGGCTGTGAATTTAGATACAGGTGAAACAATCCAAGAGGCCGCCATCGATAACTCCGATATGGCCCACAATAACTCCGTCGAGTTCCCGCCGATGGCCATCCAACCGGCGTTTCCGGCCATTGCCTCAAGCAGTGCAGGGCGGTCGACGTCGTTTAAGTCGAGGTTAGGACTTTTGACCGGGAGGAGGGGTTTTGTGGCTTGCACTCTGAGAAGGTGGTCGTCAAGTCGGAATAATAGTTAG

Protein Analysis

534

Amino Acids

57.59

Weight (kDa)

8.93

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 52 - 85 4.7e-08 F-box domain
F-box-like PF12937 52 - 85 8.2e-08 F-box-like
DUF7885 PF25372 283 - 445 1.2e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370 PF24758 298 - 407 2.4e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47056 AT4G07400
fragaria_vesca FvH4_2g33740
malus_domestica MD08G1093600.v1.1 MD15G1077300.v1.1
prunus_persica Prupe.1G430700_v2.0.a1
pyrus_communis pycom08g07610 pycom12g16540 pycom15g07200
rosa_chinensis RchiOBHm_Chr6g0308811
rosa_laevigata RLG00000010616
rosa_multiflora Rmu_sc0004046.1_g000006
rosa_roxburghii Rroxscaffold_7G00159900
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479700 Rh6BG489300 Rh6CG494800 Rh6DG480000
rosa_wichuraiana Rw6G041820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 267
AatII GACGTC 1 cut(s) 1511
AbsI CCTCGAGG 1 cut(s) 1253
AccBSI CCGCTC 1 cut(s) 629
AccI GTMKAC 2 cut(s) 135, 1505
AccII CGCG 8 cut(s) 89, 436, 450, 452, 459, 665, 716, 734
AclI AACGTT 2 cut(s) 121, 220
AclWI GGATC 8 cut(s) 60, 137, 260, 306, 369, 439, 823, 897
AcoI YGGCCR 6 cut(s) 21, 84, 867, 1263, 1455, 1476
AcsI RAATTY 2 cut(s) 28, 1369
AcuI CTGAAG 5 cut(s) 461, 745, 773, 830, 1040
AcyI GRCGYC 3 cut(s) 143, 339, 1508
AfaI GTAC 1 cut(s) 564
AfiI CCNNNNNNNGG 6 cut(s) 213, 474, 777, 1282, 1453, 1545
AgsI TTSAA 3 cut(s) 33, 511, 1210
AhdI GACNNNNNGTC 1 cut(s) 142
AjiI CACGTC 1 cut(s) 139
AjnI CCWGG 2 cut(s) 834, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 1455, 1487
Alw21I GWGCWC 2 cut(s) 536, 770
Alw26I GTCTC 4 cut(s) 281, 949, 1033, 1243
Alw44I GTGCAC 2 cut(s) 532, 766
AlwI GGATC 8 cut(s) 60, 137, 260, 306, 369, 439, 823, 897
Ama87I CYCGRG 3 cut(s) 200, 920, 1253
ApaI GGGCCC 1 cut(s) 651
ApaLI GTGCAC 2 cut(s) 532, 766
ApeKI GCWGC 4 cut(s) 103, 659, 960, 967
ApoI RAATTY 2 cut(s) 28, 1369
Asp700I GAANNNNTTC 2 cut(s) 758, 843
AspLEI GCGC 5 cut(s) 438, 452, 583, 718, 734
AspS9I GGNCC 6 cut(s) 4, 204, 647, 648, 927, 1423
AsuC2I CCSGG 2 cut(s) 651, 1540
AsuHPI GGTGA 7 cut(s) 263, 337, 610, 1150, 1204, 1297, 1394
AvaI CYCGRG 3 cut(s) 200, 920, 1253
AvaII GGWCC 2 cut(s) 204, 927
BaeGI GKGCMC 3 cut(s) 536, 651, 770
BalI TGGCCA 2 cut(s) 1265, 1457
BanII GRGCYC 2 cut(s) 651, 920
Bbv12I GWGCWC 2 cut(s) 536, 770
BbvI GCAGC 4 cut(s) 90, 646, 947, 954
BccI CCATC 7 cut(s) 69, 317, 881, 987, 1412, 1447, 1466
BceAI ACGGC 3 cut(s) 99, 326, 408
BcgI CGANNNNNNTGC 2 cut(s) 1485, 1519
BciT130I CCWGG 2 cut(s) 836, 908
BcnI CCSGG 2 cut(s) 651, 1540
BcoDI GTCTC 4 cut(s) 281, 949, 1033, 1243
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 104
BfoI RGCGCY 1 cut(s) 584
BglI GCCNNNNNGGC 1 cut(s) 646
BmcAI AGTACT 1 cut(s) 564
Bme1390I CCNGG 4 cut(s) 651, 836, 908, 1540
Bme18I GGWCC 2 cut(s) 204, 927
BmeRI GACNNNNNGTC 1 cut(s) 142
BmeT110I CYCGRG 3 cut(s) 200, 920, 1253
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 6 cut(s) 4, 204, 647, 648, 927, 1423
BmiI GGNNCC 5 cut(s) 205, 544, 608, 639, 649
BmrFI CCNGG 4 cut(s) 651, 836, 908, 1540
BmrI ACTGGG 1 cut(s) 1229
BmsI GCATC 4 cut(s) 90, 410, 460, 1164
BmuI ACTGGG 1 cut(s) 1229
BpmI CTGGAG 1 cut(s) 914
Bpu10I CCTNAGC 2 cut(s) 248, 971
BpuEI CTTGAG 2 cut(s) 1283, 1471
BpuMI CCSGG 2 cut(s) 651, 1540
Bsa29I ATCGAT 2 cut(s) 64, 1407
BsaBI GATNNNNATC 1 cut(s) 1457
BsaHI GRCGYC 3 cut(s) 143, 339, 1508
BsaI GGTCTC 1 cut(s) 949
BsaJI CCNNGG 6 cut(s) 15, 199, 610, 633, 819, 1161
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 8 cut(s) 263, 293, 1166, 1196, 1418, 1448, 1534, 1564
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 474, 777, 1282, 1453, 1545
Bse118I RCCGGY 2 cut(s) 770, 1465
Bse1I ACTGG 5 cut(s) 7, 193, 870, 1235, 1266
Bse3DI GCAATG 2 cut(s) 1185, 1479
Bse8I GATNNNNATC 1 cut(s) 1457
BseBI CCWGG 2 cut(s) 836, 908
BseCI ATCGAT 2 cut(s) 64, 1407
BseDI CCNNGG 6 cut(s) 15, 199, 610, 633, 819, 1161
BseGI GGATG 5 cut(s) 11, 312, 483, 998, 1458
BseJI GATNNNNATC 1 cut(s) 1457
BseLI CCNNNNNNNGG 6 cut(s) 213, 474, 777, 1282, 1453, 1545
BseMI GCAATG 2 cut(s) 1185, 1479
BseMII CTCAG 2 cut(s) 734, 1559
BseNI ACTGG 5 cut(s) 7, 193, 870, 1235, 1266
BseRI GAGGAG 3 cut(s) 293, 1326, 1558
BseSI GKGCMC 3 cut(s) 536, 651, 770
BseX3I CGGCCG 2 cut(s) 21, 84
BseXI GCAGC 4 cut(s) 90, 646, 947, 954
BseYI CCCAGC 1 cut(s) 473
BsgI GTGCAG 2 cut(s) 887, 1515
Bsh1236I CGCG 8 cut(s) 89, 436, 450, 452, 459, 665, 716, 734
Bsh1285I CGRYCG 6 cut(s) 24, 87, 214, 370, 645, 1505
BshVI ATCGAT 2 cut(s) 64, 1407
BsiEI CGRYCG 6 cut(s) 24, 87, 214, 370, 645, 1505
BsiHKAI GWGCWC 2 cut(s) 536, 770
BsiHKCI CYCGRG 3 cut(s) 200, 920, 1253
BsiSI CCGG 7 cut(s) 207, 214, 651, 771, 1466, 1475, 1539
BslFI GGGAC 3 cut(s) 181, 273, 794
BslI CCNNNNNNNGG 6 cut(s) 213, 474, 777, 1282, 1453, 1545
BsmAI GTCTC 4 cut(s) 281, 949, 1033, 1243
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 3 cut(s) 181, 273, 794
Bso31I GGTCTC 1 cut(s) 949
BsoBI CYCGRG 3 cut(s) 200, 920, 1253
Bsp120I GGGCCC 1 cut(s) 647
Bsp1286I GDGCHC 4 cut(s) 536, 651, 770, 920
Bsp19I CCATGG 1 cut(s) 819
BspCNI CTCAG 2 cut(s) 735, 1560
BspDI ATCGAT 2 cut(s) 64, 1407
BspFNI CGCG 8 cut(s) 89, 436, 450, 452, 459, 665, 716, 734
BspLI GGNNCC 5 cut(s) 205, 544, 608, 639, 649
BspMAI CTGCAG 1 cut(s) 108
BspPI GGATC 8 cut(s) 60, 137, 260, 306, 369, 439, 823, 897
BspQI GCTCTTC 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 949
BsrBI CCGCTC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 1185, 1479
BsrFI RCCGGY 2 cut(s) 770, 1465
BsrI ACTGG 5 cut(s) 7, 193, 870, 1235, 1266
BssAI RCCGGY 2 cut(s) 770, 1465
BssECI CCNNGG 6 cut(s) 15, 199, 610, 633, 819, 1161
BssNI GRCGYC 3 cut(s) 143, 339, 1508
BssT1I CCWWGG 1 cut(s) 819
Bst2UI CCWGG 2 cut(s) 836, 908
Bst4CI ACNGT 5 cut(s) 243, 269, 275, 708, 1162
Bst6I CTCTTC 3 cut(s) 329, 582, 764
BstACI GRCGYC 3 cut(s) 143, 339, 1508
BstC8I GCNNGC 8 cut(s) 108, 184, 354, 454, 489, 965, 1362, 1561
BstDEI CTNAG 4 cut(s) 248, 743, 971, 1568
BstDSI CCRYGG 3 cut(s) 610, 819, 1161
BstF5I GGATG 5 cut(s) 11, 312, 483, 998, 1458
BstFNI CGCG 8 cut(s) 89, 436, 450, 452, 459, 665, 716, 734
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 5 cut(s) 438, 452, 583, 718, 734
BstMAI GTCTC 4 cut(s) 281, 949, 1033, 1243
BstMCI CGRYCG 6 cut(s) 24, 87, 214, 370, 645, 1505
BstMWI GCNNNNNNNGC 8 cut(s) 45, 86, 456, 531, 646, 976, 1328, 1475
BstNI CCWGG 2 cut(s) 836, 908
BstSCI CCNGG 4 cut(s) 649, 834, 906, 1538
BstSFI CTRYAG 1 cut(s) 104
BstSLI GKGCMC 3 cut(s) 536, 651, 770
BstUI CGCG 8 cut(s) 89, 436, 450, 452, 459, 665, 716, 734
BstV1I GCAGC 4 cut(s) 90, 646, 947, 954
BstX2I RGATCY 5 cut(s) 298, 374, 444, 828, 889
BstXI CCANNNNNNTGG 1 cut(s) 886
BstYI RGATCY 5 cut(s) 298, 374, 444, 828, 889
BstZI CGGCCG 2 cut(s) 21, 84
Bsu15I ATCGAT 2 cut(s) 64, 1407
BsuTUI ATCGAT 2 cut(s) 64, 1407
BtgI CCRYGG 3 cut(s) 610, 819, 1161
BtgZI GCGATG 3 cut(s) 433, 870, 1182
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 5 cut(s) 11, 312, 483, 998, 1458
BtsI GCAGTG 1 cut(s) 1498
BtsIMutI CAGTG 3 cut(s) 713, 1273, 1498
Cac8I GCNNGC 8 cut(s) 108, 184, 354, 454, 489, 965, 1362, 1561
CciNI GCGGCCGC 1 cut(s) 21
CfoI GCGC 5 cut(s) 438, 452, 583, 718, 734
Cfr10I RCCGGY 2 cut(s) 770, 1465
Cfr13I GGNCC 6 cut(s) 4, 204, 647, 648, 927, 1423
ClaI ATCGAT 2 cut(s) 64, 1407
CseI GACGC 4 cut(s) 132, 347, 373, 654
CsiI ACCWGGT 1 cut(s) 906
Csp6I GTAC 1 cut(s) 563
CviAII CATG 2 cut(s) 820, 1282
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 4 cut(s) 248, 743, 971, 1568
DrdI GACNNNNNNGTC 1 cut(s) 267
DriI GACNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 267
EaeI YGGCCR 6 cut(s) 21, 84, 867, 1263, 1455, 1476
EagI CGGCCG 2 cut(s) 21, 84
Eam1104I CTCTTC 3 cut(s) 329, 582, 764
Eam1105I GACNNNNNGTC 1 cut(s) 142
EarI CTCTTC 3 cut(s) 329, 582, 764
EclXI CGGCCG 2 cut(s) 21, 84
Eco130I CCWWGG 1 cut(s) 819
Eco24I GRGCYC 2 cut(s) 651, 920
Eco31I GGTCTC 1 cut(s) 949
Eco47I GGWCC 2 cut(s) 204, 927
Eco52I CGGCCG 2 cut(s) 21, 84
Eco57I CTGAAG 5 cut(s) 461, 745, 773, 830, 1040
Eco88I CYCGRG 3 cut(s) 200, 920, 1253
EcoRII CCWGG 2 cut(s) 834, 906
EcoT14I CCWWGG 1 cut(s) 819
EcoT38I GRGCYC 2 cut(s) 651, 920
ErhI CCWWGG 1 cut(s) 819
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 2 cut(s) 823, 1285
FaiI YATR 6 cut(s) 188, 416, 821, 1223, 1283, 1421
FalI AAGNNNNNCTT 2 cut(s) 1339, 1371
FaqI GGGAC 3 cut(s) 181, 273, 794
FatI CATG 2 cut(s) 819, 1281
FauI CCCGC 3 cut(s) 338, 518, 1455
FblI GTMKAC 2 cut(s) 135, 1505
FokI GGATG 4 cut(s) 299, 490, 1005, 1445
FriOI GRGCYC 2 cut(s) 651, 920
FspBI CTAG 1 cut(s) 566
GlaI GCGC 5 cut(s) 437, 451, 582, 717, 733
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 1 cut(s) 914
HaeII RGCGCY 1 cut(s) 584
HapII CCGG 7 cut(s) 207, 214, 651, 771, 1466, 1475, 1539
HgaI GACGC 4 cut(s) 132, 347, 373, 654
HhaI GCGC 5 cut(s) 438, 452, 583, 718, 734
Hin1I GRCGYC 3 cut(s) 143, 339, 1508
Hin1II CATG 2 cut(s) 823, 1285
Hin6I GCGC 5 cut(s) 436, 450, 581, 716, 732
HinP1I GCGC 5 cut(s) 436, 450, 581, 716, 732
HincII GTYRAC 3 cut(s) 136, 465, 1506
HindII GTYRAC 3 cut(s) 136, 465, 1506
HindIII AAGCTT 2 cut(s) 842, 977
HinfI GANTC 3 cut(s) 748, 898, 1081
HpaII CCGG 7 cut(s) 207, 214, 651, 771, 1466, 1475, 1539
HphI GGTGA 7 cut(s) 263, 337, 610, 1150, 1204, 1297, 1394
Hpy166II GTNNAC 6 cut(s) 136, 465, 534, 768, 927, 1506
Hpy188III TCNNGA 7 cut(s) 167, 893, 913, 1058, 1130, 1240, 1300
Hpy8I GTNNAC 6 cut(s) 136, 465, 534, 768, 927, 1506
HpyAV CCTTC 6 cut(s) 327, 542, 991, 1064, 1319, 1566
HpyCH4III ACNGT 5 cut(s) 243, 269, 275, 708, 1162
HpyCH4IV ACGT 5 cut(s) 121, 138, 220, 536, 1508
HpyF10VI GCNNNNNNNGC 8 cut(s) 45, 86, 456, 531, 646, 976, 1328, 1475
HpyF3I CTNAG 4 cut(s) 248, 743, 971, 1568
HpySE526I ACGT 5 cut(s) 121, 138, 220, 536, 1508
Hsp92I GRCGYC 3 cut(s) 143, 339, 1508
Hsp92II CATG 2 cut(s) 823, 1285
HspAI GCGC 5 cut(s) 436, 450, 581, 716, 732
LguI GCTCTTC 1 cut(s) 764
LmnI GCTCC 6 cut(s) 542, 612, 626, 637, 1052, 1328
Lsp1109I GCAGC 4 cut(s) 90, 646, 947, 954
LweI GCATC 4 cut(s) 90, 410, 460, 1164
MabI ACCWGGT 1 cut(s) 906
MaeI CTAG 1 cut(s) 566
MaeII ACGT 5 cut(s) 121, 138, 220, 536, 1508
MaeIII GTNAC 5 cut(s) 269, 343, 425, 811, 1315
MbiI CCGCTC 1 cut(s) 629
MboII GAAGA 8 cut(s) 155, 316, 454, 523, 599, 748, 751, 1222
MfeI CAATTG 3 cut(s) 571, 880, 1117
MflI RGATCY 5 cut(s) 298, 374, 444, 828, 889
MhlI GDGCHC 4 cut(s) 536, 651, 770, 920
MlsI TGGCCA 2 cut(s) 1265, 1457
MluCI AATT 6 cut(s) 28, 571, 880, 1061, 1117, 1369
MluNI TGGCCA 2 cut(s) 1265, 1457
MlyI GAGTC 1 cut(s) 742
MmeI TCCRAC 4 cut(s) 1074, 1109, 1486, 1568
Mox20I TGGCCA 2 cut(s) 1265, 1457
MroXI GAANNNNTTC 2 cut(s) 758, 843
MscI TGGCCA 2 cut(s) 1265, 1457
MseI TTAA 2 cut(s) 152, 1515
MslI CAYNNNNRTG 3 cut(s) 413, 1160, 1190
Msp20I TGGCCA 2 cut(s) 1265, 1457
MspI CCGG 7 cut(s) 207, 214, 651, 771, 1466, 1475, 1539
MspR9I CCNGG 4 cut(s) 651, 836, 908, 1540
MunI CAATTG 3 cut(s) 571, 880, 1117
MvaI CCWGG 2 cut(s) 836, 908
MvnI CGCG 8 cut(s) 89, 436, 450, 452, 459, 665, 716, 734
MwoI GCNNNNNNNGC 8 cut(s) 45, 86, 456, 531, 646, 976, 1328, 1475
NciI CCSGG 2 cut(s) 651, 1540
NcoI CCATGG 1 cut(s) 819
NlaIII CATG 2 cut(s) 823, 1285
NlaIV GGNNCC 5 cut(s) 205, 544, 608, 639, 649
NmeAIII GCCGAG 2 cut(s) 557, 829
NmuCI GTSAC 3 cut(s) 269, 343, 811
NotI GCGGCCGC 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 1253
PciSI GCTCTTC 1 cut(s) 764
PcsI WCGNNNNNNNCGW 6 cut(s) 144, 207, 399, 674, 1413, 1517
PdmI GAANNNNTTC 2 cut(s) 758, 843
PfeI GAWTC 2 cut(s) 898, 1081
PflFI GACNNNGTC 1 cut(s) 341
Ple19I CGATCG 1 cut(s) 645
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
Psp1406I AACGTT 2 cut(s) 121, 220
Psp6I CCWGG 2 cut(s) 834, 906
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 2 cut(s) 834, 906
PspN4I GGNNCC 5 cut(s) 205, 544, 608, 639, 649
PspOMI GGGCCC 1 cut(s) 647
PspPI GGNCC 6 cut(s) 4, 204, 647, 648, 927, 1423
PspXI VCTCGAGB 1 cut(s) 1253
PstI CTGCAG 1 cut(s) 108
PsuI RGATCY 5 cut(s) 298, 374, 444, 828, 889
PsyI GACNNNGTC 1 cut(s) 341
PvuI CGATCG 1 cut(s) 645
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
RseI CAYNNNNRTG 3 cut(s) 413, 1160, 1190
SalI GTCGAC 2 cut(s) 134, 1504
SapI GCTCTTC 1 cut(s) 764
SaqAI TTAA 2 cut(s) 152, 1515
Sau96I GGNCC 6 cut(s) 4, 204, 647, 648, 927, 1423
ScaI AGTACT 1 cut(s) 564
SchI GAGTC 1 cut(s) 742
ScrFI CCNGG 4 cut(s) 651, 836, 908, 1540
SduI GDGCHC 4 cut(s) 536, 651, 770, 920
SexAI ACCWGGT 1 cut(s) 906
SfaNI GCATC 4 cut(s) 90, 410, 460, 1164
SfcI CTRYAG 1 cut(s) 104
Sfr274I CTCGAG 1 cut(s) 1253
SgrAI CRCCGGYG 1 cut(s) 770
SgrDI CGTCGACG 1 cut(s) 134
SinI GGWCC 2 cut(s) 204, 927
SlaI CTCGAG 1 cut(s) 1253
SmiMI CAYNNNNRTG 3 cut(s) 413, 1160, 1190
SmlI CTYRAG 3 cut(s) 1253, 1298, 1486
SmoI CTYRAG 3 cut(s) 1253, 1298, 1486
Sse9I AATT 6 cut(s) 28, 571, 880, 1061, 1117, 1369
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 4 cut(s) 649, 834, 906, 1538
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 5 cut(s) 243, 269, 275, 708, 1162
TaiI ACGT 5 cut(s) 124, 141, 223, 539, 1511
TaqII GACCGA 1 cut(s) 482
TasI AATT 6 cut(s) 28, 571, 880, 1061, 1117, 1369
TatI WGTACW 1 cut(s) 562
TfiI GAWTC 2 cut(s) 898, 1081
Tru1I TTAA 2 cut(s) 152, 1515
Tru9I TTAA 2 cut(s) 152, 1515
TscAI CASTG 3 cut(s) 713, 1273, 1498
TseFI GTSAC 3 cut(s) 269, 343, 811
TseI GCWGC 4 cut(s) 103, 659, 960, 967
Tsp45I GTSAC 3 cut(s) 269, 343, 811
TspDTI ATGAA 1 cut(s) 569
TspGWI ACGGA 6 cut(s) 367, 599, 639, 937, 1005, 1426
TspRI CASTG 3 cut(s) 713, 1273, 1498
Tth111I GACNNNGTC 1 cut(s) 341
VneI GTGCAC 2 cut(s) 532, 766
VpaK11BI GGWCC 2 cut(s) 204, 927
XapI RAATTY 2 cut(s) 28, 1369
XcmI CCANNNNNNNNNTGG 2 cut(s) 937, 1096
XhoI CTCGAG 1 cut(s) 1253
XmiI GTMKAC 2 cut(s) 135, 1505
XmnI GAANNNNTTC 2 cut(s) 758, 843
XspI CTAG 1 cut(s) 566
ZraI GACGTC 1 cut(s) 1509
ZrmI AGTACT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.