AT4G07400

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012273
Physical Location & Seq
Reverse (-)
4197666 .. 4199511
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G07400.1

Sequence Viewer

Length: 1665 bp
ATGAATATTTACATTTTTAGCAAAGAAAAAAAAAGAAAAAAAGTTGTTTGCGACAATTTATCCAAAACCAGAGGGAGTAGGCGGGAACATTGTGAACCGCATCGCCGTCGAATGGGCCAATCCACTTCCAAATTCCGGCGATCTAAAACTACCTTCACTTCTCCGGTACTACCTAATTTACGGGAACAAAACTCTGGTGCCGATGAGCCGTACGATTACATTTCTAACTTACCAGACGAATGCTTATCTTTAATTTTCCAATCGCTAACTTGCGCCGACCTAAAACGTTGCTCACTGGTGTGTCGTCGGTGGTTAACAATTGAAGGACAGTGTCGTCATCGTCTCTCTCTCAAAGCACAATCAGATCTTATTTCAGTGATACCATCTCTATTTACTCGGTTTGATTCTGTTACTAAACTTGTTCTTAGAAGTGACCGTAGATCACTTGGTATATGTGACAACGCATTCGTTATGATTTCTGTTAGATGTCGGAATTTAACGCGGTTGAAGTTACGCGGTTGTCCGGAGATTTCGGATTTAGGGATTATAGGTTTCACTGAGAATTGTAGGAGTTTGAAGAAGGTTTCATTTGGATCTTGTGGGTTTGGTGTTAAAGGAATGAATGCTTTGTTGAATACTTGTTTAGGTCTAGAGGAATTGTCGGTTAAACGGTTACGCGGAATCGGTGCTGGTGCTGAGCTTATAGGACCTGGGGGAGCTGCGGGTTCGTTGAAGGTTATTTGCTTAAAGGAGCTGCATAATGGTCAGTGTTTTGCTCCATTGTTAAGTGGAGCTAAAGGACTTAGGATTTTGAAGATTTTCAGGTGTTCAGGGGATTGGGATCGGGTTTTTGAGGCGGTTCGTGATAAGGTTAACGCTATTGTTGAGATACATTTAGAGAGGATTCAGATGAGTGATTTGGGTTTAACCGCGTTATCGAAATGTTCGGGTGTTGAGGTTCTGCATCTTGTGAAAACTCCTGATTGTACGAATGTTGGTTTAGCTTTGGTGGCTGAGCGTTGTAAATTGCTAAGGAAGCTTCATATTGATGGATGGAAGACGAATCGAATAGGCGATGAGGGGCTTATAGTTGTTGCTAAGTACTGTTGGAATTTGCAGGAGTTGGTTTTGATTGGTGTGAATCCTACTAAGTTAAGTTTGGAAGCGATTGTTTCGAATTGTCTTAACTTGGAGAGATTGGCATTGTGTGGAAGTGATACGGTAGGTGACACTGAGTTATGTTGTATTGCTGAGAAATGTTTGGCGTTGAGGAAGCTTTGTATTAAGAACTGTCCTATTACTGATGATGGCATCAAAGCTCTTGGGAACGGATGTCCGAATCTGCTAAAAGTGAAGGTTAAGAAGTGTAGAGGAGTGACTACTCAAGGAGCTGATTTGTTAAGGAAAAGAAGGGCGTTGCTTGTTGTCAATTTAGATGCGCCCGAGACACCAATTGTTGAGGGTAGTGTCGGTGAAGGTGGAGCACAGGAGAATGCAGTTGAGTTCCCTCCTTCCAGACTCCAAATTCCAACCATAGGCCTTGCATCTGGTAGCACCAGCCGGTCATCCTCGTTTAAGTTACGATTGGGGTTTTTGAGCCAGAGGAACTTTGTTTCTTGTGCACTGAGAAGGTTGGGAAGTCGAAGCAGGTCCCGGAATGAATAG

Protein Analysis

554

Amino Acids

61.0

Weight (kDa)

9.5

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 74 - 105 7.2e-09 F-box-like
F-box PF00646 74 - 106 3.9e-08 F-box domain
DUF7885 PF25372 305 - 469 1.6e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47056 AT4G07400
fragaria_vesca FvH4_2g33740
malus_domestica MD08G1093600.v1.1 MD15G1077300.v1.1
prunus_persica Prupe.1G430700_v2.0.a1
pyrus_communis pycom08g07610 pycom12g16540 pycom15g07200
rosa_chinensis RchiOBHm_Chr6g0308811
rosa_laevigata RLG00000010616
rosa_multiflora Rmu_sc0004046.1_g000006
rosa_roxburghii Rroxscaffold_7G00159900
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479700 Rh6BG489300 Rh6CG494800 Rh6DG480000
rosa_wichuraiana Rw6G041820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 333
Acc36I ACCTGC 1 cut(s) 1638
AccB1I GGYRCC 1 cut(s) 197
AccII CGCG 4 cut(s) 502, 516, 678, 932
AccIII TCCGGA 1 cut(s) 523
AciI CCGC 8 cut(s) 82, 98, 502, 516, 678, 722, 857, 930
AclI AACGTT 1 cut(s) 286
AclWI GGATC 2 cut(s) 601, 849
AcsI RAATTY 4 cut(s) 131, 493, 1111, 1524
AfaI GTAC 4 cut(s) 168, 212, 988, 1103
AfiI CCNNNNNNNGG 3 cut(s) 112, 135, 1535
AgsI TTSAA 6 cut(s) 323, 508, 577, 634, 733, 814
AjnI CCWGG 1 cut(s) 709
AleI CACNNNNGTG 1 cut(s) 298
AluBI AGCT 9 cut(s) 700, 719, 754, 794, 1004, 1039, 1276, 1319, 1391
AluI AGCT 9 cut(s) 700, 719, 754, 794, 1004, 1039, 1276, 1319, 1391
Alw21I GWGCWC 2 cut(s) 1486, 1624
Alw26I GTCTC 2 cut(s) 347, 1439
Alw44I GTGCAC 1 cut(s) 1620
AlwI GGATC 2 cut(s) 601, 849
Ama87I CYCGRG 1 cut(s) 1442
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 2 cut(s) 115, 1537
ApaLI GTGCAC 1 cut(s) 1620
ApeKI GCWGC 2 cut(s) 719, 754
ApoI RAATTY 4 cut(s) 131, 493, 1111, 1524
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
Asp700I GAANNNNTTC 1 cut(s) 818
AspLEI GCGC 2 cut(s) 275, 1441
AspS9I GGNCC 3 cut(s) 115, 707, 1650
AsuC2I CCSGG 1 cut(s) 1654
AsuHPI GGTGA 2 cut(s) 1238, 1484
AsuII TTCGAA 1 cut(s) 1175
AvaI CYCGRG 1 cut(s) 1442
AvaII GGWCC 2 cut(s) 707, 1650
BaeGI GKGCMC 1 cut(s) 1624
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 1064
Bbv12I GWGCWC 2 cut(s) 1486, 1624
BbvI GCAGC 2 cut(s) 706, 741
BccI CCATC 4 cut(s) 391, 1043, 1047, 1301
BceAI ACGGC 2 cut(s) 90, 193
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BciT130I CCWGG 1 cut(s) 711
BcnI CCSGG 1 cut(s) 1654
BcoDI GTCTC 2 cut(s) 347, 1439
BfaI CTAG 1 cut(s) 650
BfuAI ACCTGC 1 cut(s) 1638
BglII AGATCT 1 cut(s) 364
BisI GCNGC 2 cut(s) 720, 755
BlpI GCTNAGC 2 cut(s) 696, 1014
BlsI GCNGC 2 cut(s) 721, 756
BmcAI AGTACT 1 cut(s) 1103
Bme1390I CCNGG 2 cut(s) 711, 1654
Bme18I GGWCC 2 cut(s) 707, 1650
BmeT110I CYCGRG 1 cut(s) 1442
BmgT120I GGNCC 3 cut(s) 115, 707, 1650
BmiI GGNNCC 2 cut(s) 199, 1652
BmrFI CCNGG 2 cut(s) 711, 1654
BmsI GCATC 5 cut(s) 109, 973, 1320, 1426, 1553
BpiI GAAGAC 1 cut(s) 1064
Bpu10I CCTNAGC 1 cut(s) 1031
Bpu1102I GCTNAGC 2 cut(s) 696, 1014
Bpu14I TTCGAA 1 cut(s) 1175
BpuEI CTTGAG 1 cut(s) 1368
BpuMI CCSGG 1 cut(s) 1654
BsaBI GATNNNNATC 1 cut(s) 840
BsaJI CCNNGG 1 cut(s) 710
BsaWI WCCGGW 2 cut(s) 163, 523
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 135, 1535
Bse118I RCCGGY 1 cut(s) 1560
Bse1I ACTGG 1 cut(s) 300
Bse8I GATNNNNATC 1 cut(s) 840
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 3 cut(s) 1058, 1337, 1565
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 3 cut(s) 112, 135, 1535
BseMII CTCAG 6 cut(s) 549, 687, 1005, 1224, 1242, 1616
BseNI ACTGG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 1386
BseSI GKGCMC 1 cut(s) 1624
BseXI GCAGC 2 cut(s) 706, 741
Bsh1236I CGCG 4 cut(s) 502, 516, 678, 932
BshFI GGCC 2 cut(s) 117, 1539
BshNI GGYRCC 1 cut(s) 197
BsiHKAI GWGCWC 2 cut(s) 1486, 1624
BsiHKCI CYCGRG 1 cut(s) 1442
BsiSI CCGG 5 cut(s) 136, 164, 524, 1561, 1654
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 1636
BslI CCNNNNNNNGG 3 cut(s) 112, 135, 1535
BsmAI GTCTC 2 cut(s) 347, 1439
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 1636
BsmI GAATGC 4 cut(s) 245, 464, 628, 1498
BsnI GGCC 2 cut(s) 117, 1539
BsoBI CYCGRG 1 cut(s) 1442
Bsp119I TTCGAA 1 cut(s) 1175
Bsp1286I GDGCHC 2 cut(s) 1486, 1624
Bsp13I TCCGGA 1 cut(s) 523
Bsp143I GATC 5 cut(s) 140, 364, 440, 593, 841
Bsp1720I GCTNAGC 2 cut(s) 696, 1014
BspACI CCGC 8 cut(s) 82, 98, 502, 516, 678, 722, 857, 930
BspANI GGCC 2 cut(s) 117, 1539
BspCNI CTCAG 6 cut(s) 550, 688, 1006, 1225, 1243, 1617
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 4 cut(s) 502, 516, 678, 932
BspLI GGNNCC 2 cut(s) 199, 1652
BspMI ACCTGC 1 cut(s) 1638
BspPI GGATC 2 cut(s) 601, 849
BspT104I TTCGAA 1 cut(s) 1175
BspT107I GGYRCC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 1560
BsrI ACTGG 1 cut(s) 300
BssAI RCCGGY 1 cut(s) 1560
BssECI CCNNGG 1 cut(s) 710
BssMI GATC 5 cut(s) 140, 364, 440, 593, 841
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 6 cut(s) 330, 437, 672, 1106, 1222, 1292
BstBI TTCGAA 1 cut(s) 1175
BstF5I GGATG 3 cut(s) 1058, 1337, 1565
BstFNI CGCG 4 cut(s) 502, 516, 678, 932
BstHHI GCGC 2 cut(s) 275, 1441
BstKTI GATC 5 cut(s) 143, 367, 443, 596, 844
BstMAI GTCTC 2 cut(s) 347, 1439
BstMBI GATC 5 cut(s) 140, 364, 440, 593, 841
BstMWI GCNNNNNNNGC 2 cut(s) 1010, 1036
BstNI CCWGG 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 709, 1652
BstSLI GKGCMC 1 cut(s) 1624
BstUI CGCG 4 cut(s) 502, 516, 678, 932
BstV1I GCAGC 2 cut(s) 706, 741
BstV2I GAAGAC 1 cut(s) 1064
BstX2I RGATCY 2 cut(s) 364, 593
BstYI RGATCY 2 cut(s) 364, 593
BsuRI GGCC 2 cut(s) 117, 1539
BtgZI GCGATG 2 cut(s) 86, 1089
BtsCI GGATG 3 cut(s) 1058, 1337, 1565
BtsIMutI CAGTG 7 cut(s) 293, 335, 381, 555, 773, 1230, 1622
BveI ACCTGC 1 cut(s) 1638
CfoI GCGC 2 cut(s) 275, 1441
Cfr10I RCCGGY 1 cut(s) 1560
Cfr13I GGNCC 3 cut(s) 115, 707, 1650
Csp6I GTAC 4 cut(s) 167, 211, 987, 1102
CviQI GTAC 4 cut(s) 167, 211, 987, 1102
DpnI GATC 5 cut(s) 142, 366, 442, 595, 843
DpnII GATC 5 cut(s) 140, 364, 440, 593, 841
DrdI GACNNNNNNGTC 1 cut(s) 333
DseDI GACNNNNNNGTC 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 1539
Eco47I GGWCC 2 cut(s) 707, 1650
Eco88I CYCGRG 1 cut(s) 1442
EcoO109I RGGNCCY 2 cut(s) 707, 1650
EcoRII CCWGG 1 cut(s) 709
Esp3I CGTCTC 1 cut(s) 347
FaqI GGGAC 1 cut(s) 1636
FauI CCCGC 2 cut(s) 75, 715
Fnu4HI GCNGC 2 cut(s) 720, 755
FokI GGATG 3 cut(s) 1065, 1344, 1552
Fsp4HI GCNGC 2 cut(s) 720, 755
FspBI CTAG 1 cut(s) 650
GlaI GCGC 2 cut(s) 274, 1440
GluI GCNGC 2 cut(s) 720, 755
HaeIII GGCC 2 cut(s) 117, 1539
HapII CCGG 5 cut(s) 136, 164, 524, 1561, 1654
HhaI GCGC 2 cut(s) 275, 1441
Hin6I GCGC 2 cut(s) 273, 1439
HinP1I GCGC 2 cut(s) 273, 1439
HincII GTYRAC 2 cut(s) 315, 874
HindII GTYRAC 2 cut(s) 315, 874
HindIII AAGCTT 2 cut(s) 1037, 1274
HinfI GANTC 7 cut(s) 404, 681, 904, 1063, 1141, 1339, 1518
HpaI GTTAAC 2 cut(s) 315, 874
HpaII CCGG 5 cut(s) 136, 164, 524, 1561, 1654
HphI GGTGA 2 cut(s) 1238, 1484
Hpy166II GTNNAC 4 cut(s) 95, 315, 874, 1622
Hpy188I TCNGA 5 cut(s) 364, 492, 535, 909, 1338
Hpy188III TCNNGA 5 cut(s) 524, 650, 863, 980, 1515
Hpy8I GTNNAC 4 cut(s) 95, 315, 874, 1622
Hpy99I CGWCG 2 cut(s) 111, 309
HpyAV CCTTC 9 cut(s) 163, 317, 574, 727, 1348, 1404, 1469, 1521, 1623
HpyCH4III ACNGT 6 cut(s) 330, 437, 672, 1106, 1222, 1292
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 6 cut(s) 757, 964, 1117, 1496, 1544, 1622
HpyF10VI GCNNNNNNNGC 2 cut(s) 1010, 1036
HpySE526I ACGT 1 cut(s) 286
HspAI GCGC 2 cut(s) 273, 1439
Kpn2I TCCGGA 1 cut(s) 523
KspAI GTTAAC 2 cut(s) 315, 874
Kzo9I GATC 5 cut(s) 140, 364, 440, 593, 841
LmnI GCTCC 6 cut(s) 716, 751, 781, 791, 1388, 1481
Lsp1109I GCAGC 2 cut(s) 706, 741
LweI GCATC 5 cut(s) 109, 973, 1320, 1426, 1553
MaeI CTAG 1 cut(s) 650
MaeII ACGT 1 cut(s) 286
MaeIII GTNAC 8 cut(s) 409, 431, 455, 510, 672, 1226, 1375, 1578
MalI GATC 5 cut(s) 142, 366, 442, 595, 843
MboI GATC 5 cut(s) 140, 364, 440, 593, 841
MboII GAAGA 3 cut(s) 589, 826, 1069
MfeI CAATTG 2 cut(s) 318, 1452
MflI RGATCY 2 cut(s) 364, 593
MhlI GDGCHC 2 cut(s) 1486, 1624
MlyI GAGTC 1 cut(s) 1512
MmeI TCCRAC 3 cut(s) 470, 1088, 1553
MroI TCCGGA 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 818
MslI CAYNNNNRTG 2 cut(s) 298, 1047
MspI CCGG 5 cut(s) 136, 164, 524, 1561, 1654
MspR9I CCNGG 2 cut(s) 711, 1654
MunI CAATTG 2 cut(s) 318, 1452
Mva1269I GAATGC 4 cut(s) 245, 464, 628, 1498
MvaI CCWGG 1 cut(s) 711
MvnI CGCG 4 cut(s) 502, 516, 678, 932
MwoI GCNNNNNNNGC 2 cut(s) 1010, 1036
NciI CCSGG 1 cut(s) 1654
NdeII GATC 5 cut(s) 140, 364, 440, 593, 841
NlaIV GGNNCC 2 cut(s) 199, 1652
NmuCI GTSAC 4 cut(s) 431, 455, 1226, 1375
NspV TTCGAA 1 cut(s) 1175
OliI CACNNNNGTG 1 cut(s) 298
PceI AGGCCT 1 cut(s) 1539
PctI GAATGC 4 cut(s) 245, 464, 628, 1498
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 6 cut(s) 404, 681, 904, 1063, 1141, 1339
Pfl23II CGTACG 1 cut(s) 210
PflFI GACNNNGTC 1 cut(s) 330
PfoI TCCNGGA 1 cut(s) 1652
PkrI GCNGC 2 cut(s) 721, 756
PleI GAGTC 1 cut(s) 1512
PpsI GAGTC 1 cut(s) 1512
PpuMI RGGWCCY 2 cut(s) 707, 1650
Psp1406I AACGTT 1 cut(s) 286
Psp5II RGGWCCY 2 cut(s) 707, 1650
Psp6I CCWGG 1 cut(s) 709
PspGI CCWGG 1 cut(s) 709
PspLI CGTACG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 199, 1652
PspPI GGNCC 3 cut(s) 115, 707, 1650
PspPPI RGGWCCY 2 cut(s) 707, 1650
PsuI RGATCY 2 cut(s) 364, 593
PsyI GACNNNGTC 1 cut(s) 330
RsaI GTAC 4 cut(s) 168, 212, 988, 1103
RsaNI GTAC 4 cut(s) 167, 211, 987, 1102
RseI CAYNNNNRTG 2 cut(s) 298, 1047
SatI GCNGC 2 cut(s) 720, 755
Sau3AI GATC 5 cut(s) 140, 364, 440, 593, 841
Sau96I GGNCC 3 cut(s) 115, 707, 1650
ScaI AGTACT 1 cut(s) 1103
SchI GAGTC 1 cut(s) 1512
ScrFI CCNGG 2 cut(s) 711, 1654
SduI GDGCHC 2 cut(s) 1486, 1624
SfaNI GCATC 5 cut(s) 109, 973, 1320, 1426, 1553
SfuI TTCGAA 1 cut(s) 1175
SinI GGWCC 2 cut(s) 707, 1650
SmiMI CAYNNNNRTG 2 cut(s) 298, 1047
SmlI CTYRAG 1 cut(s) 1383
SmoI CTYRAG 1 cut(s) 1383
SseBI AGGCCT 1 cut(s) 1539
SsiI CCGC 8 cut(s) 82, 98, 502, 516, 678, 722, 857, 930
SspI AATATT 1 cut(s) 7
SspMI CTAG 1 cut(s) 650
StuI AGGCCT 1 cut(s) 1539
StyD4I CCNGG 2 cut(s) 709, 1652
TaaI ACNGT 6 cut(s) 330, 437, 672, 1106, 1222, 1292
TaiI ACGT 1 cut(s) 289
TaqI TCGA 5 cut(s) 109, 938, 1066, 1175, 1642
TatI WGTACW 1 cut(s) 1101
TfiI GAWTC 6 cut(s) 404, 681, 904, 1063, 1141, 1339
TscAI CASTG 7 cut(s) 300, 335, 381, 562, 773, 1237, 1629
TseFI GTSAC 4 cut(s) 431, 455, 1226, 1375
TseI GCWGC 2 cut(s) 719, 754
Tsp45I GTSAC 4 cut(s) 431, 455, 1226, 1375
TspDTI ATGAA 4 cut(s) 17, 576, 635, 1031
TspGWI ACGGA 1 cut(s) 1344
TspRI CASTG 7 cut(s) 300, 335, 381, 562, 773, 1237, 1629
Tth111I GACNNNGTC 1 cut(s) 330
VneI GTGCAC 1 cut(s) 1620
VpaK11BI GGWCC 2 cut(s) 707, 1650
XapI RAATTY 4 cut(s) 131, 493, 1111, 1524
XbaI TCTAGA 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 1 cut(s) 650
ZrmI AGTACT 1 cut(s) 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.