Rh6BG489300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012273
Physical Location & Seq
Forward (+)
69333115 .. 69333888
774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG489300.1

Sequence Viewer

Length: 774 bp
ATGGTTGAAATCCATCTGGAAAAGCTTCAGGTTAGCGACGTTGCGCTGGCCGCAATCTCCAATTGCTTGGATCTGGAAATTTTACACCTGGTCAAAACGCCGGACTGCACAAATGTAGGTCTGATTTCGGTCTCGGAACGCTGCAAGCTCTTGAGGAAGCTTCACATTGACGGCTGGAAGTCAAACCGTGTGGGAGATGAAGGCTTGATTTCGGTGGCCAAGAGTTGCCCCAACCTTCAGGAATTGGTTCTTATCGGCGTGAATCCAACAAAAGCGAGCTTGGAAACGTTGGCGGCGAATTGCCCCAATCTGGAGAGGTTGGCATTGTGCGGGAGTGATACAGTAGGCGATCCGGAGCTTTTGTGCATTGCTGCGAAATGTGGAGCATTGAAGAAGCTTTGCATTAAGAGCTGCCCTGTGTCTGATGTTGGCCTCGAAGGACTGGCCAGTGGGTGCCCTAATATGGTGAAAGTGAAGGTTAAGAAGTGTAGGCTAGTAACACCCGAGGGTGCTGATTCTTTGAGGGCAAAGAGGCCTACATTGGCTGTGAATTTGGACACCGGTGAGCCTCCACAAGACGCCAGTGCCAGTGACGGAGGGGCACAAGATAACCCTGCTGTCGAATTCCTTCCTCTGGCCACACAACCAGTGTTTCCTGACATTGCTTCAAGCAGTACAGGGAGGTCTGCGTCCTTTAAGTCAAGGTTAAGCCTTTTATCTGGAAGGACCCTTGTGGCTTGCACTCTGAGAAGGTGGGTAAGTCGGAATAGTTAG

Protein Analysis

257

Amino Acids

27.19

Weight (kDa)

8.21

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 26 - 113 5.9e-08 FBD-associated F-box protein At5g56370, LRR repeats
DUF7885 PF25372 97 - 175 3.1e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47056 AT4G07400
fragaria_vesca FvH4_2g33740
malus_domestica MD08G1093600.v1.1 MD15G1077300.v1.1
prunus_persica Prupe.1G430700_v2.0.a1
pyrus_communis pycom08g07610 pycom12g16540 pycom15g07200
rosa_chinensis RchiOBHm_Chr6g0308811
rosa_laevigata RLG00000010616
rosa_multiflora Rmu_sc0004046.1_g000006
rosa_roxburghii Rroxscaffold_7G00159900
rosa_rugosa Rorug06G0368300
rosa_samantha Rh6AG479700 Rh6BG489300 Rh6CG494800 Rh6DG480000
rosa_wichuraiana Rw6G041820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 453
AccIII TCCGGA 1 cut(s) 352
AciI CCGC 3 cut(s) 51, 293, 330
AclI AACGTT 1 cut(s) 287
AclWI GGATC 2 cut(s) 78, 344
AcoI YGGCCR 4 cut(s) 48, 216, 444, 636
AcsI RAATTY 3 cut(s) 78, 550, 623
AcuI CTGAAG 2 cut(s) 11, 221
AcyI GRCGYC 1 cut(s) 579
AfaI GTAC 1 cut(s) 676
AfiI CCNNNNNNNGG 3 cut(s) 310, 463, 634
AgeI ACCGGT 1 cut(s) 560
AgsI TTSAA 3 cut(s) 8, 391, 669
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 7 cut(s) 25, 148, 160, 279, 358, 397, 411
AluI AGCT 7 cut(s) 25, 148, 160, 279, 358, 397, 411
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 2 cut(s) 78, 344
Ama87I CYCGRG 1 cut(s) 503
Aor13HI TCCGGA 1 cut(s) 352
AoxI GGCC 6 cut(s) 48, 216, 430, 444, 533, 636
ApeKI GCWGC 3 cut(s) 141, 371, 411
ApoI RAATTY 3 cut(s) 78, 550, 623
AsiGI ACCGGT 1 cut(s) 560
Asp700I GAANNNNTTC 3 cut(s) 24, 246, 627
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 726
AsuHPI GGTGA 2 cut(s) 478, 575
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 1 cut(s) 726
BaeGI GKGCMC 2 cut(s) 458, 604
BalI TGGCCA 3 cut(s) 218, 446, 638
BanI GGYRCC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 128, 358, 398
BccI CCATC 1 cut(s) 21
BceAI ACGGC 1 cut(s) 187
BciT130I CCWGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 1 cut(s) 494
BisI GCNGC 5 cut(s) 51, 142, 294, 372, 412
BlsI GCNGC 5 cut(s) 52, 143, 295, 373, 413
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 726
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 1 cut(s) 726
BmiI GGNNCC 2 cut(s) 455, 728
BmrFI CCNGG 1 cut(s) 89
BpmI CTGGAG 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 172
BsaHI GRCGYC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 1 cut(s) 504
BsaWI WCCGGW 2 cut(s) 352, 560
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 463, 634
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 5 cut(s) 447, 447, 582, 588, 647
Bse3DI GCAATG 2 cut(s) 366, 660
BseAI TCCGGA 1 cut(s) 352
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 504
BseLI CCNNNNNNNGG 3 cut(s) 310, 463, 634
BseMI GCAATG 2 cut(s) 366, 660
BseMII CTCAG 1 cut(s) 737
BseNI ACTGG 5 cut(s) 447, 447, 582, 588, 647
BseSI GKGCMC 2 cut(s) 458, 604
BseXI GCAGC 3 cut(s) 128, 358, 398
BsgI GTGCAG 1 cut(s) 91
BshFI GGCC 6 cut(s) 50, 218, 432, 446, 535, 638
BshNI GGYRCC 1 cut(s) 453
BshTI ACCGGT 1 cut(s) 560
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 3 cut(s) 101, 353, 561
BslI CCNNNNNNNGG 3 cut(s) 310, 463, 634
BsmAI GTCTC 1 cut(s) 136
BsnI GGCC 6 cut(s) 50, 218, 432, 446, 535, 638
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 1 cut(s) 503
Bsp1286I GDGCHC 2 cut(s) 458, 604
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 2 cut(s) 70, 349
BspACI CCGC 3 cut(s) 51, 293, 330
BspANI GGCC 6 cut(s) 50, 218, 432, 446, 535, 638
BspCNI CTCAG 1 cut(s) 738
BspEI TCCGGA 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 455, 728
BspPI GGATC 2 cut(s) 78, 344
BspT107I GGYRCC 1 cut(s) 453
BspTNI GGTCTC 1 cut(s) 136
BsrDI GCAATG 2 cut(s) 366, 660
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 5 cut(s) 447, 447, 582, 588, 647
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 2 cut(s) 70, 349
BssNI GRCGYC 1 cut(s) 579
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 2 cut(s) 188, 343
BstACI GRCGYC 1 cut(s) 579
BstC8I GCNNGC 4 cut(s) 48, 146, 277, 739
BstDEI CTNAG 1 cut(s) 746
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 2 cut(s) 73, 352
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 2 cut(s) 70, 349
BstMWI GCNNNNNNNGC 2 cut(s) 50, 408
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstSLI GKGCMC 2 cut(s) 458, 604
BstV1I GCAGC 3 cut(s) 128, 358, 398
BstX2I RGATCY 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 6 cut(s) 50, 218, 432, 446, 535, 638
BtsIMutI CAGTG 4 cut(s) 454, 589, 595, 654
Cac8I GCNNGC 4 cut(s) 48, 146, 277, 739
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 1 cut(s) 726
CseI GACGC 2 cut(s) 587, 678
CsiI ACCWGGT 1 cut(s) 87
Csp6I GTAC 1 cut(s) 675
CspAI ACCGGT 1 cut(s) 560
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviQI GTAC 1 cut(s) 675
DdeI CTNAG 1 cut(s) 746
DpnI GATC 2 cut(s) 72, 351
DpnII GATC 2 cut(s) 70, 349
EaeI YGGCCR 4 cut(s) 48, 216, 444, 636
Eco147I AGGCCT 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 726
Eco57I CTGAAG 2 cut(s) 11, 221
Eco88I CYCGRG 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 726
EcoRI GAATTC 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 87
FaiI YATR 1 cut(s) 464
FauI CCCGC 1 cut(s) 323
Fnu4HI GCNGC 5 cut(s) 51, 142, 294, 372, 412
Fsp4HI GCNGC 5 cut(s) 51, 142, 294, 372, 412
FspBI CTAG 1 cut(s) 494
GlaI GCGC 1 cut(s) 45
GluI GCNGC 5 cut(s) 51, 142, 294, 372, 412
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 6 cut(s) 50, 218, 432, 446, 535, 638
HapII CCGG 3 cut(s) 101, 353, 561
HgaI GACGC 2 cut(s) 587, 678
HhaI GCGC 1 cut(s) 46
Hin1I GRCGYC 1 cut(s) 579
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HindIII AAGCTT 3 cut(s) 23, 158, 395
HinfI GANTC 2 cut(s) 262, 515
HpaII CCGG 3 cut(s) 101, 353, 561
HphI GGTGA 2 cut(s) 478, 575
Hpy188I TCNGA 5 cut(s) 123, 136, 424, 747, 765
Hpy188III TCNNGA 8 cut(s) 17, 74, 151, 239, 311, 353, 656, 720
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 7 cut(s) 194, 245, 431, 469, 638, 717, 744
HpyCH4III ACNGT 2 cut(s) 188, 343
HpyCH4IV ACGT 2 cut(s) 39, 287
HpyCH4V TGCA 5 cut(s) 108, 144, 366, 402, 741
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 408
HpyF3I CTNAG 1 cut(s) 746
HpySE526I ACGT 2 cut(s) 39, 287
Hsp92I GRCGYC 1 cut(s) 579
HspAI GCGC 1 cut(s) 44
Kpn2I TCCGGA 1 cut(s) 352
Kzo9I GATC 2 cut(s) 70, 349
LmnI GCTCC 2 cut(s) 355, 383
Lsp1109I GCAGC 3 cut(s) 128, 358, 398
MabI ACCWGGT 1 cut(s) 87
MaeI CTAG 1 cut(s) 494
MaeII ACGT 2 cut(s) 39, 287
MaeIII GTNAC 2 cut(s) 496, 590
MalI GATC 2 cut(s) 72, 351
MboI GATC 2 cut(s) 70, 349
MboII GAAGA 1 cut(s) 403
MfeI CAATTG 1 cut(s) 61
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 2 cut(s) 458, 604
MlsI TGGCCA 3 cut(s) 218, 446, 638
MluCI AATT 6 cut(s) 61, 78, 242, 298, 550, 623
MluNI TGGCCA 3 cut(s) 218, 446, 638
MmeI TCCRAC 2 cut(s) 290, 743
Mox20I TGGCCA 3 cut(s) 218, 446, 638
MroI TCCGGA 1 cut(s) 352
MroXI GAANNNNTTC 3 cut(s) 24, 246, 627
MscI TGGCCA 3 cut(s) 218, 446, 638
MseI TTAA 4 cut(s) 405, 480, 696, 707
Msp20I TGGCCA 3 cut(s) 218, 446, 638
MspI CCGG 3 cut(s) 101, 353, 561
MspR9I CCNGG 1 cut(s) 89
MunI CAATTG 1 cut(s) 61
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 50, 408
NdeII GATC 2 cut(s) 70, 349
NlaIV GGNNCC 2 cut(s) 455, 728
NmuCI GTSAC 1 cut(s) 590
PceI AGGCCT 1 cut(s) 535
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PdmI GAANNNNTTC 3 cut(s) 24, 246, 627
PfeI GAWTC 2 cut(s) 262, 515
PinAI ACCGGT 1 cut(s) 560
PkrI GCNGC 5 cut(s) 52, 143, 295, 373, 413
PpuMI RGGWCCY 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 287
Psp5II RGGWCCY 1 cut(s) 726
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 455, 728
PspPI GGNCC 1 cut(s) 726
PspPPI RGGWCCY 1 cut(s) 726
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
SaqAI TTAA 4 cut(s) 405, 480, 696, 707
SatI GCNGC 5 cut(s) 51, 142, 294, 372, 412
Sau3AI GATC 2 cut(s) 70, 349
Sau96I GGNCC 1 cut(s) 726
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 2 cut(s) 458, 604
SexAI ACCWGGT 1 cut(s) 87
SgrAI CRCCGGYG 1 cut(s) 560
SinI GGWCC 1 cut(s) 726
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 6 cut(s) 61, 78, 242, 298, 550, 623
SseBI AGGCCT 1 cut(s) 535
SsiI CCGC 3 cut(s) 51, 293, 330
SspMI CTAG 1 cut(s) 494
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 188, 343
TaiI ACGT 2 cut(s) 42, 290
TaqI TCGA 2 cut(s) 435, 621
TaqII GACCGA 1 cut(s) 118
TasI AATT 6 cut(s) 61, 78, 242, 298, 550, 623
TatI WGTACW 1 cut(s) 674
TauI GCSGC 2 cut(s) 53, 296
TfiI GAWTC 2 cut(s) 262, 515
Tru1I TTAA 4 cut(s) 405, 480, 696, 707
Tru9I TTAA 4 cut(s) 405, 480, 696, 707
TscAI CASTG 4 cut(s) 454, 589, 595, 654
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 3 cut(s) 141, 371, 411
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 1 cut(s) 213
TspGWI ACGGA 1 cut(s) 609
TspRI CASTG 4 cut(s) 454, 589, 595, 654
VpaK11BI GGWCC 1 cut(s) 726
XapI RAATTY 3 cut(s) 78, 550, 623
XmnI GAANNNNTTC 3 cut(s) 24, 246, 627
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.