AT1G49040

DENN domain and WD repeat-containing protein SCD1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007797
Physical Location & Seq
Reverse (-)
18139174 .. 18149019
9846 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49040.1

Sequence Viewer

Length: 3564 bp
ATGGGACGGATCTTCGAGTACTTCGTCGTGTGCGGACTTGGTCCAGAGATGCGAACCGTGGATGGAGATCTTGGATTCCATGGAATGCAAACATTCTACTTGCCTGCTCTTCTTGACCAGTTCCCTCCTACTGATCAATCTCCTTACCCTGCCCCTCCTCCTCAGCTTCCTACTTGTGTTCTTCCTGCTGGGGTGGAGTTCCATTCTTCTGGTTTTGTTTCCAGTGACCCTGCAAGTTTCCCACGGAGCTATCCCATAGTCTTGACGGAGGGTGATGGATCAAAAATATTTGTCAGTTGCATTGCATTTCGGGATCGAGTCTGTGAGGATATTATTGAAGCTTATCGCTTACCGCCGAATACATACGCAGACAAGTGTATCTGTCTTGTGTCTCATGCCCCCAATTTCCGTGTTCTTCGTAATTCTCTCGAAGAGATTTTTGTTCTTTGCTTCTCTTCAGAAGGAAGCTGTAAGCCATTGTGGGATATTATCGCCTATATGGTATCTAATGTGCCATTGCCGACTCCCGGAAAAGACAGAGTCTTGTTTGCTGTCGAGAATTGCCTGCTCTCGGTGGAAGCACCTCCAGAGGATAGTCTTCCTCAGGCAGATATATCTCTCCAGCCTCTTGTACAATGCTTGGATGTCGACAACTTGATTAAGCTGTTTACATCTGTACTAGTTGAAAGGAGGATTTTGATTCGATCCAACAAGTATTCGCTTTTGACTCTGGTGTCTGAATCCATATGTCATTTGATTTATCCTTTTCGATGGCTGCAAGTCTACATTCCATTACTATTTTTTAGTGGAGTAGACTACATTGATGCACCAACACCATATATGATGGGTCTCCACTCTGATGTCGATACATCCAATCTTGCTATGGATGGCGTTGTAGTGGTGGATCTTGACATAAACCAAATTACTACTTCTGAAGAGATACCTCAGATACCAGAGCCTGAGTTCAGCACTTTGCGAAACGATATCTTGAAACTACTGCACCCTAATGTTGTAGCGATTGATCAGTTGAAGGGCTTTGGTAATTCCGTTGAGCAGTGCCCTAAAAGCCTTAGCAAACCTTGGGGAGAGGATCATGACCTTCAACTCAGGGTTATATTCTTAAAGTGTTTTGCATCTATCTTGGGTGGCTACCGCAATTTCATAGAAAACAAAGTTTTTAGCACTGATGCGTTTCTGAAGAGACGGTCTCGTTCCACAAATCAGCCACCAGAGCCTATGTTGGTTCAATTCTTGGGCTCTTTTGCATTCCTCGATTACCTTGAAAGGCGTCTAAGCTCTGATGAGAAGAGTACTAATCTCCTTGAGAAGTTACAAGATGCAGTTGGAAGGGGTCAAGATGCAATGTCGATTCTGCCTAAATCTTCTATGGAGCCTGAAATAATCACAATAGCCGAGCCAGAAGTTGAAGAATCAGCCACCAGGTATACCTATGACAGGTTTCCTGCGAGTGTTCGGTCAGAGGAACAAGAAGAAAAGCGAAAGCAGATCCTTGCAGCAGCAAGCGGGGCTCTTGAATCCAATGGAAGGCACCCTCCAAGCTCACCGCCAGGGAAGAACACCAAAGAAGACAATTTCAGTTCAATGGAGAGGGCTGCAGAAAGAGAACGAATGGTCTTGGATATACAAGTCAAGTTGCAGGGATTATGGCTCCGTCTTCTGAAACTGGGTTCAGATGAAGATCCTCTTTCTTCATTCGAATACGGCACAATATTGGCTCTGATTGAATCTGATGCTGAGGGGATTGGTGGGAGTGGCTTTATCGAGTGTATACGAGAGCATCTGTATTCGGGCTGGCATGGTCAATTGTCCGAGGAGCAGTTCATTGCGGTCAAAGAATTGCTCAAAATGGCTGTGGGCCGTGCTGCTTCAAGAAGCGATTTGTCGACAGTGCGGGATGCACTTGAAGTTTCTGCAGAAATGTTCAAGAAGGATGCCAATAATGTCTCTGACTATGTTCAGCGTCATCTCATCTCCATACCCATCTGGGAGGAGTTGAGATTTTGGGAAGGTTACTTCGAGTATCTCATGGAGCAGCCTGCAAATGAATCTGTGAACTATGCTACTTTGGTCACAGCCAGACTAATCATAGTGGCATCACATATGGCTGGCTTGGGACTTCCTGATACAGAAGCTTGGAACATGATCGAGACAATAGCAGAGAAACAAAAGCTTGGATATAAATTACTGATTAAACTTAGAGGGTTCCTGTCGCATGTTCAGCAACTTCGTGTCGGCTATTGGGGTGCTTCTTCATTTAAGCAGCAGGCCATATCTGCTGGGTTGCCATCCCCGCGTCCAAAAGATGTCTCTGTCTCTGATGAAACCCAGCAACCCTCAGAAGCTTCTGGAAGGAGTTGGGTTCAGAGTATGTTTAGCAGAGATACAGCATCGAGAGCGAACTCGTTCAGTCGTGTTCGTAAGTGGGTATCTGATAATGCATCTTCAGACATAACTGCTGCTGCGCAGAAGAAAATTCAGACCAATGTACGTGTTCTGAAGGGTCACGGTGGTGCTGTCACTGCCTTGCACTCTGTGACAAGAAGGGAAGTCTGTGATCTTGTGGGCGATCGCGAGGATGCTGGGTTCTTTATCAGCGGTAGTACAGACTGTCTGGTTAAAATTTGGGATCCAAGTCTGCGTGGTTCTGAACTTCGAGCAACTTTGAAAGGACATACTGGAACCGTGCGTGCTATAAGCTCTGACAGAGGAAAGATAGTTTCAGGATCAGATGATCTATCTGTGATCGTATGGGACAAACAGACAACTCAACTTCTAGAAGAACTGAAAGGCCATGATTCACAGGTAAGTTGTGTCAAGATGCTTTCAGGCGAACGTGTCCTCACTGCTGCACATGATGGAACAGTTAAGATGTGGGACGTTCGAACCGATATGTGTGTTGCAACTGTTGGCCGATGCTCTAGTGCCATTCTTTCACTAGAATATGATGACTCCACAGGAATCTTGGCTGCTGCTGGCAGAGATACGGTTGCAAATATATGGGATATTCGTTCAGGAAAGCAAATGCATAAGCTGAAAGGGCATACCAAATGGATACGATCAATTCGAATGGTCGAAGATACCTTGATTACTGGAAGTGATGACTGGACAGCAAGAGTGTGGTCTGTCTCCAGAGGATCATGCGATGCTGTTTTGGCATGCCATGCTGGGCCAGTACAATCTGTTGAATACTCCCCGTTTGACAAAGGAATAATCACAGGCTCGGCGGATGGGTTGCTACGCTTTTGGGAAAATGACGAAGGCGGCATTAAATGCGTGAAAAACATAACACTCCATAGTTCTTCTATACTATCAATCAACGCTGGTGAAAATTGGTTAGGCATTGGAGCAGCAGATAACTCAATGTCTCTTTTCCATCGTCCTTCTAACGCCGGAACCAAAGTCTCAGGCTGGCAACTTTATAGAGTACCACAAAGAACAGCCGCCGTGGTGAGATGTGTTGCGTCAGATCTCGAGAGGAAACGGATATGTAGCGGTGGCCGAAATGGAGTTCTCCGGCTGTGGGATGCCACCATCAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000278 GO:0000281 GO:0000819 GO:0000910 GO:0000911 GO:0001568 GO:0001570 GO:0001932 GO:0001934 GO:0001944 GO:0002682 GO:0002683 GO:0002761 GO:0002762 GO:0003674 GO:0003700 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005739 GO:0005783 GO:0005794 GO:0005886 GO:0006275 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007154 GO:0007165 GO:0007166 GO:0007176 GO:0007219 GO:0007275 GO:0008150 GO:0008152 GO:0008156 GO:0009056 GO:0009057 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009653 GO:0009791 GO:0009825 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009967 GO:0009987 GO:0010235 GO:0010374 GO:0010440 GO:0010468 GO:0010469 GO:0010498 GO:0010506 GO:0010556 GO:0010558 GO:0010562 GO:0010564 GO:0010594 GO:0010604 GO:0010605 GO:0010632 GO:0010638 GO:0010646 GO:0010647 GO:0010721 GO:0010821 GO:0010822 GO:0010941 GO:0010942 GO:0010948 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016049 GO:0016567 GO:0016740 GO:0019005 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0022402 GO:0022603 GO:0023051 GO:0023052 GO:0023056 GO:0030135 GO:0030136 GO:0030154 GO:0030162 GO:0030163 GO:0030234 GO:0030323 GO:0030324 GO:0030332 GO:0030334 GO:0030674 GO:0031146 GO:0031224 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0031347 GO:0031348 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031410 GO:0031461 GO:0031625 GO:0031647 GO:0031648 GO:0031974 GO:0031981 GO:0031982 GO:0032268 GO:0032270 GO:0032386 GO:0032388 GO:0032446 GO:0032501 GO:0032502 GO:0032506 GO:0032875 GO:0032876 GO:0032879 GO:0032880 GO:0032991 GO:0033043 GO:0033157 GO:0033554 GO:0033674 GO:0034644 GO:0035239 GO:0035295 GO:0036211 GO:0040007 GO:0040012 GO:0042058 GO:0042176 GO:0042325 GO:0042327 GO:0042802 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043392 GO:0043408 GO:0043410 GO:0043412 GO:0043523 GO:0043525 GO:0043535 GO:0043549 GO:0043632 GO:0044092 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045309 GO:0045595 GO:0045596 GO:0045637 GO:0045638 GO:0045670 GO:0045671 GO:0045732 GO:0045741 GO:0045742 GO:0045765 GO:0045786 GO:0045824 GO:0045859 GO:0045860 GO:0045862 GO:0045934 GO:0045937 GO:0048471 GO:0048513 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048731 GO:0048856 GO:0048869 GO:0050730 GO:0050731 GO:0050776 GO:0050777 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050816 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051052 GO:0051053 GO:0051093 GO:0051098 GO:0051100 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051219 GO:0051222 GO:0051223 GO:0051239 GO:0051241 GO:0051246 GO:0051247 GO:0051252 GO:0051270 GO:0051276 GO:0051301 GO:0051338 GO:0051347 GO:0051438 GO:0051443 GO:0051603 GO:0051716 GO:0051726 GO:0055106 GO:0060090 GO:0060255 GO:0060284 GO:0060341 GO:0060541 GO:0061097 GO:0061098 GO:0061136 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070201 GO:0070372 GO:0070374 GO:0070647 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071840 GO:0071944 GO:0072358 GO:0072359 GO:0080008 GO:0080090 GO:0080134 GO:0080135 GO:0090049 GO:0090087 GO:0090316 GO:0090329 GO:0090558 GO:0097027 GO:0097708 GO:0098772 GO:0098813 GO:0104004 GO:0140096 GO:0140110 GO:1900407 GO:1900409 GO:1901184 GO:1901186 GO:1901214 GO:1901216 GO:1901342 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1902105 GO:1902106 GO:1902175 GO:1902177 GO:1902410 GO:1902494 GO:1902531 GO:1902533 GO:1902882 GO:1902884 GO:1903010 GO:1903011 GO:1903025 GO:1903026 GO:1903047 GO:1903050 GO:1903052 GO:1903146 GO:1903201 GO:1903203 GO:1903209 GO:1903214 GO:1903223 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1903376 GO:1903378 GO:1903506 GO:1903533 GO:1903670 GO:1903706 GO:1903707 GO:1903747 GO:1903749 GO:1903827 GO:1903829 GO:1903955 GO:1904951 GO:1990234 GO:1990452 GO:2000026 GO:2000058 GO:2000060 GO:2000104 GO:2000112 GO:2000113 GO:2000145 GO:2000273 GO:2000677 GO:2000678 GO:2001141 GO:2001204 GO:2001205 GO:2001233 GO:2001235 GO:2001242 GO:2001244
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DENN WD40 dDENN uDENN

Protein Analysis

1187

Amino Acids

131.59

Weight (kDa)

5.53

Isoelectric Point (pI)

47.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
uDENN PF03456 35 - 103 2e-11 uDENN domain
DENN PF02141 123 - 304 8.1e-43 DENN (AEX-3) domain
Beta-prop_TEP1_2nd PF25047 807 - 967 1.2e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 865 - 966 1.1e-07 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 865 - 1048 1.1e-08 MABP1/WDR62 second WD40 domain
Beta-prop_WDR5 PF25175 866 - 1012 8.8e-26 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 869 - 1018 2.2e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd PF25047 869 - 1066 9.8e-18 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 870 - 970 7.4e-07 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 872 - 999 1.2e-06 MABP1/WDR62 first WD40 domain
WDR55 PF24796 874 - 1013 3.9e-15 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 887 - 1090 2.6e-15 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 888 - 983 6.6e-19 WDR3 first beta-propeller domain
Beta-prop_WDR41 PF25178 893 - 986 3.7e-08 WDR41
WD40_WDHD1_1st PF24817 898 - 976 2.7e-09 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 906 - 988 4.2e-12 THOC3 beta-propeller domain
WD40_Prp19 PF24814 907 - 986 6e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 908 - 1054 1.6e-15 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 935 - 1131 2.2e-15 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 936 - 1083 4.9e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_DCAF4 PF23761 938 - 1015 3.1e-09 DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_EML PF23409 960 - 1135 1e-05 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_2nd PF25172 979 - 1134 5.7e-11 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 980 - 1113 4.9e-23 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 981 - 1090 1.6e-21 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 981 - 1184 2.1e-19 THOC3 beta-propeller domain
WD40_Prp19 PF24814 982 - 1095 7.9e-18 Prp19 WD40 domain
EIF3I PF24805 983 - 1073 4.2e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 1016 - 1102 5.6e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 1032 - 1135 1.2e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 1033 - 1108 2.2e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 1035 - 1131 3.6e-10 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 733
Acc16I TGCGCA 1 cut(s) 2484
AccB1I GGYRCC 1 cut(s) 1548
AccI GTMKAC 6 cut(s) 648, 783, 813, 1445, 1789, 1904
AccII CGCG 2 cut(s) 2314, 2592
AcoI YGGCCR 2 cut(s) 2929, 3517
AcsI RAATTY 2 cut(s) 2493, 2640
AcuI CTGAAG 5 cut(s) 441, 954, 1217, 2448, 2537
AcyI GRCGYC 1 cut(s) 1288
AdeI CACNNNGTG 1 cut(s) 2554
AfaI GTAC 8 cut(s) 20, 633, 678, 1312, 2508, 2623, 3195, 3447
AfiI CCNNNNNNNGG 5 cut(s) 527, 571, 1455, 1545, 3541
AflIII ACRYGT 2 cut(s) 2509, 2854
AhlI ACTAGT 1 cut(s) 679
AjnI CCWGG 2 cut(s) 1439, 1567
AleI CACNNNNGTG 1 cut(s) 2529
AloI GAACNNNNNNTCC 2 cut(s) 2588, 2620
AlwNI CAGNNNCTG 1 cut(s) 959
Ama87I CYCGRG 1 cut(s) 3491
AoxI GGCC 6 cut(s) 1876, 2286, 2809, 2929, 3188, 3517
ApoI RAATTY 2 cut(s) 2493, 2640
AsiSI GCGATCGC 1 cut(s) 2590
Asp700I GAANNNNTTC 1 cut(s) 2423
AspLEI GCGC 1 cut(s) 2485
AspS9I GGNCC 3 cut(s) 41, 1876, 3188
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 4 cut(s) 284, 1554, 3356, 3481
AsuII TTCGAA 3 cut(s) 1716, 2902, 3085
AvaI CYCGRG 1 cut(s) 3491
AvaII GGWCC 1 cut(s) 41
AxyI CCTNAGG 1 cut(s) 603
BaeGI GKGCMC 1 cut(s) 1061
BaeI ACNNNNGTAYC 2 cut(s) 361, 394
BamHI GGATCC 1 cut(s) 2647
BanI GGYRCC 1 cut(s) 1548
BanII GRGCYC 2 cut(s) 1259, 1531
BbsI GAAGAC 3 cut(s) 590, 1593, 1667
BbvCI CCTCAGC 2 cut(s) 162, 1755
BceAI ACGGC 3 cut(s) 1738, 1863, 3449
BciT130I CCWGG 2 cut(s) 1441, 1569
BciVI GTATCC 1 cut(s) 3066
BclI TGATCA 2 cut(s) 133, 1021
BcnI CCSGG 1 cut(s) 528
BcuI ACTAGT 1 cut(s) 679
BfaI CTAG 4 cut(s) 680, 2795, 2940, 2957
BfmI CTRYAG 2 cut(s) 1614, 1932
BfuI GTATCC 1 cut(s) 3066
BglII AGATCT 2 cut(s) 67, 3487
BmcAI AGTACT 2 cut(s) 20, 1312
Bme1390I CCNGG 3 cut(s) 528, 1441, 1569
Bme18I GGWCC 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 3491
BmgT120I GGNCC 3 cut(s) 41, 1876, 3188
BmiI GGNNCC 7 cut(s) 1392, 1550, 1670, 2225, 2649, 2701, 3415
BmrFI CCNGG 3 cut(s) 528, 1441, 1569
BmrI ACTGGG 1 cut(s) 1694
BmuI ACTGGG 1 cut(s) 1694
BpiI GAAGAC 3 cut(s) 590, 1593, 1667
BplI GAGNNNNNCTC 2 cut(s) 1192, 1224
BpmI CTGGAG 3 cut(s) 570, 605, 3133
Bpu10I CCTNAGC 3 cut(s) 162, 1070, 1755
Bpu14I TTCGAA 3 cut(s) 1716, 2902, 3085
BpuEI CTTGAG 1 cut(s) 1343
BpuMI CCSGG 1 cut(s) 528
BsaAI YACGTR 1 cut(s) 2510
BsaBI GATNNNNATC 2 cut(s) 66, 1698
BsaHI GRCGYC 1 cut(s) 1288
BsaI GGTCTC 2 cut(s) 854, 1212
BsaJI CCNNGG 7 cut(s) 57, 79, 242, 1079, 1568, 1830, 3465
Bsc4I CCNNNNNNNGG 5 cut(s) 527, 571, 1455, 1545, 3541
Bse1I ACTGG 7 cut(s) 118, 222, 1689, 2701, 3115, 3128, 3191
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 4 cut(s) 300, 515, 1368, 1842
Bse8I GATNNNNATC 2 cut(s) 66, 1698
BseBI CCWGG 2 cut(s) 1441, 1569
BseDI CCNNGG 7 cut(s) 57, 79, 242, 1079, 1568, 1830, 3465
BseJI GATNNNNATC 2 cut(s) 66, 1698
BseLI CCNNNNNNNGG 5 cut(s) 527, 571, 1455, 1545, 3541
BseMI GCAATG 4 cut(s) 300, 515, 1368, 1842
BseMII CTCAG 8 cut(s) 176, 617, 951, 959, 1120, 1746, 2370, 3438
BseNI ACTGG 7 cut(s) 118, 222, 1689, 2701, 3115, 3128, 3191
BseRI GAGGAG 4 cut(s) 147, 150, 1847, 2024
BseSI GKGCMC 1 cut(s) 1061
BseYI CCCAGC 5 cut(s) 188, 2297, 2346, 2600, 3185
BsgI GTGCAG 2 cut(s) 983, 2853
Bsh1236I CGCG 2 cut(s) 2314, 2592
Bsh1285I CGRYCG 1 cut(s) 2590
BshFI GGCC 6 cut(s) 1878, 2288, 2811, 2931, 3190, 3519
BshNI GGYRCC 1 cut(s) 1548
BsiEI CGRYCG 1 cut(s) 2590
BsiHKCI CYCGRG 1 cut(s) 3491
BsiSI CCGG 3 cut(s) 528, 3411, 3535
BslFI GGGAC 4 cut(s) 18, 2148, 2786, 2909
BslI CCNNNNNNNGG 5 cut(s) 527, 571, 1455, 1545, 3541
BsmBI CGTCTC 1 cut(s) 1195
BsmFI GGGAC 4 cut(s) 18, 2148, 2786, 2909
BsmI GAATGC 2 cut(s) 90, 1265
BsnI GGCC 6 cut(s) 1878, 2288, 2811, 2931, 3190, 3519
Bso31I GGTCTC 2 cut(s) 854, 1212
BsoBI CYCGRG 1 cut(s) 3491
Bsp119I TTCGAA 3 cut(s) 1716, 2902, 3085
Bsp1286I GDGCHC 3 cut(s) 1061, 1259, 1531
Bsp1407I TGTACA 1 cut(s) 631
Bsp19I CCATGG 1 cut(s) 79
Bsp68I TCGCGA 1 cut(s) 2592
BspANI GGCC 6 cut(s) 1878, 2288, 2811, 2931, 3190, 3519
BspCNI CTCAG 8 cut(s) 175, 616, 952, 958, 1119, 1747, 2369, 3437
BspFNI CGCG 2 cut(s) 2314, 2592
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 7 cut(s) 1392, 1550, 1670, 2225, 2649, 2701, 3415
BspMAI CTGCAG 2 cut(s) 1618, 1936
BspQI GCTCTTC 1 cut(s) 114
BspT104I TTCGAA 3 cut(s) 1716, 2902, 3085
BspT107I GGYRCC 1 cut(s) 1548
BspTNI GGTCTC 2 cut(s) 854, 1212
BsrDI GCAATG 4 cut(s) 300, 515, 1368, 1842
BsrGI TGTACA 1 cut(s) 631
BsrI ACTGG 7 cut(s) 118, 222, 1689, 2701, 3115, 3128, 3191
BssECI CCNNGG 7 cut(s) 57, 79, 242, 1079, 1568, 1830, 3465
BssNAI GTATAC 2 cut(s) 1446, 1790
BssNI GRCGYC 1 cut(s) 1288
BssT1I CCWWGG 2 cut(s) 79, 1079
Bst1107I GTATAC 2 cut(s) 1446, 1790
Bst2UI CCWGG 2 cut(s) 1441, 1569
Bst4CI ACNGT 9 cut(s) 58, 1206, 1909, 2528, 2630, 2704, 2884, 2926, 3007
Bst6I CTCTTC 6 cut(s) 114, 426, 460, 930, 1193, 1301
BstACI GRCGYC 1 cut(s) 1288
BstAPI GCANNNNNTGC 2 cut(s) 3182, 3291
BstAUI TGTACA 1 cut(s) 631
BstBAI YACGTR 1 cut(s) 2510
BstBI TTCGAA 3 cut(s) 1716, 2902, 3085
BstDSI CCRYGG 4 cut(s) 57, 79, 242, 3465
BstENI CCTNNNNNAGG 1 cut(s) 1453
BstFNI CGCG 2 cut(s) 2314, 2592
BstHHI GCGC 1 cut(s) 2485
BstMCI CGRYCG 1 cut(s) 2590
BstNI CCWGG 2 cut(s) 1441, 1569
BstNSI RCATGY 2 cut(s) 2237, 3180
BstSCI CCNGG 3 cut(s) 526, 1439, 1567
BstSFI CTRYAG 2 cut(s) 1614, 1932
BstSLI GKGCMC 1 cut(s) 1061
BstUI CGCG 2 cut(s) 2314, 2592
BstV2I GAAGAC 3 cut(s) 590, 1593, 1667
BstX2I RGATCY 7 cut(s) 9, 67, 904, 1506, 1699, 2647, 3487
BstXI CCANNNNNNTGG 1 cut(s) 209
BstYI RGATCY 7 cut(s) 9, 67, 904, 1506, 1699, 2647, 3487
BstZ17I GTATAC 2 cut(s) 1446, 1790
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 1 cut(s) 3066
BsuRI GGCC 6 cut(s) 1878, 2288, 2811, 2931, 3190, 3519
BtgI CCRYGG 4 cut(s) 57, 79, 242, 3465
BtgZI GCGATG 1 cut(s) 3177
BtsI GCAGTG 3 cut(s) 1061, 2538, 2862
BtsIMutI CAGTG 6 cut(s) 229, 1061, 1182, 1914, 2538, 2862
BtuMI TCGCGA 1 cut(s) 2592
CaiI CAGNNNCTG 1 cut(s) 959
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 1 cut(s) 2485
Cfr13I GGNCC 3 cut(s) 41, 1876, 3188
CseI GACGC 4 cut(s) 1277, 1970, 2303, 3471
CsiI ACCWGGT 1 cut(s) 1439
Csp6I GTAC 8 cut(s) 19, 632, 677, 1311, 2507, 2622, 3194, 3446
CviQI GTAC 8 cut(s) 19, 632, 677, 1311, 2507, 2622, 3194, 3446
DraIII CACNNNGTG 1 cut(s) 2554
DrdI GACNNNNNNGTC 1 cut(s) 733
DseDI GACNNNNNNGTC 1 cut(s) 733
EaeI YGGCCR 2 cut(s) 2929, 3517
Eam1104I CTCTTC 6 cut(s) 114, 426, 460, 930, 1193, 1301
EarI CTCTTC 6 cut(s) 114, 426, 460, 930, 1193, 1301
EciI GGCGGA 1 cut(s) 3260
Eco130I CCWWGG 2 cut(s) 79, 1079
Eco24I GRGCYC 2 cut(s) 1259, 1531
Eco31I GGTCTC 2 cut(s) 854, 1212
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 1 cut(s) 41
Eco57I CTGAAG 5 cut(s) 441, 954, 1217, 2448, 2537
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 3491
EcoNI CCTNNNNNAGG 1 cut(s) 1453
EcoRII CCWGG 2 cut(s) 1439, 1567
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 2 cut(s) 79, 1079
EcoT22I ATGCAT 2 cut(s) 2461, 3048
EcoT38I GRGCYC 2 cut(s) 1259, 1531
ErhI CCWWGG 2 cut(s) 79, 1079
Esp3I CGTCTC 1 cut(s) 1195
FalI AAGNNNNNCTT 2 cut(s) 1689, 1721
FaqI GGGAC 4 cut(s) 18, 2148, 2786, 2909
FauI CCCGC 3 cut(s) 1517, 1905, 2319
FauNDI CATATG 2 cut(s) 746, 2121
FbaI TGATCA 2 cut(s) 133, 1021
FblI GTMKAC 6 cut(s) 648, 783, 813, 1445, 1789, 1904
FriOI GRGCYC 2 cut(s) 1259, 1531
FspBI CTAG 4 cut(s) 680, 2795, 2940, 2957
FspI TGCGCA 1 cut(s) 2484
GlaI GCGC 1 cut(s) 2484
GsaI CCCAGC 5 cut(s) 192, 2301, 2350, 2604, 3189
GsuI CTGGAG 3 cut(s) 570, 605, 3133
HaeIII GGCC 6 cut(s) 1878, 2288, 2811, 2931, 3190, 3519
HapII CCGG 3 cut(s) 528, 3411, 3535
HgaI GACGC 4 cut(s) 1277, 1970, 2303, 3471
HhaI GCGC 1 cut(s) 2485
Hin1I GRCGYC 1 cut(s) 1288
Hin6I GCGC 1 cut(s) 2483
HinP1I GCGC 1 cut(s) 2483
HincII GTYRAC 2 cut(s) 649, 1905
HindII GTYRAC 2 cut(s) 649, 1905
HindIII AAGCTT 4 cut(s) 339, 2151, 2189, 2361
HpaII CCGG 3 cut(s) 528, 3411, 3535
HphI GGTGA 4 cut(s) 284, 1554, 3356, 3481
Hpy166II GTNNAC 8 cut(s) 649, 669, 784, 814, 1446, 1790, 1905, 2074
Hpy8I GTNNAC 8 cut(s) 649, 669, 784, 814, 1446, 1790, 1905, 2074
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 9 cut(s) 58, 1206, 1909, 2528, 2630, 2704, 2884, 2926, 3007
HpyCH4IV ACGT 3 cut(s) 2509, 2854, 2898
HpySE526I ACGT 3 cut(s) 2509, 2854, 2898
Hsp92I GRCGYC 1 cut(s) 1288
HspAI GCGC 1 cut(s) 2483
Ksp22I TGATCA 2 cut(s) 133, 1021
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 6 cut(s) 246, 1390, 1674, 1834, 2050, 3365
MabI ACCWGGT 1 cut(s) 1439
MaeI CTAG 4 cut(s) 680, 2795, 2940, 2957
MaeII ACGT 3 cut(s) 2509, 2854, 2898
MaeIII GTNAC 7 cut(s) 224, 1329, 2030, 2089, 2522, 2536, 2554
MfeI CAATTG 1 cut(s) 1823
MflI RGATCY 7 cut(s) 9, 67, 904, 1506, 1699, 2647, 3487
MhlI GDGCHC 3 cut(s) 1061, 1259, 1531
MlyI GAGTC 5 cut(s) 327, 517, 549, 721, 2963
MmeI TCCRAC 2 cut(s) 732, 1324
Mph1103I ATGCAT 2 cut(s) 2461, 3048
MroXI GAANNNNTTC 1 cut(s) 2423
MseI TTAA 7 cut(s) 660, 1121, 2211, 2277, 2637, 2886, 3288
MslI CAYNNNNRTG 3 cut(s) 858, 1005, 2529
MspA1I CMGCKG 1 cut(s) 2616
MspI CCGG 3 cut(s) 528, 3411, 3535
MspR9I CCNGG 3 cut(s) 528, 1441, 1569
MunI CAATTG 1 cut(s) 1823
Mva1269I GAATGC 2 cut(s) 90, 1265
MvaI CCWGG 2 cut(s) 1441, 1569
MvnI CGCG 2 cut(s) 2314, 2592
NciI CCSGG 1 cut(s) 528
NcoI CCATGG 1 cut(s) 79
NdeI CATATG 2 cut(s) 746, 2121
NlaIV GGNNCC 7 cut(s) 1392, 1550, 1670, 2225, 2649, 2701, 3415
NmeAIII GCCGAG 2 cut(s) 1438, 3221
NmuCI GTSAC 5 cut(s) 224, 2089, 2522, 2536, 2554
NruI TCGCGA 1 cut(s) 2592
NsbI TGCGCA 1 cut(s) 2484
NsiI ATGCAT 2 cut(s) 2461, 3048
NspI RCATGY 2 cut(s) 2237, 3180
NspV TTCGAA 3 cut(s) 1716, 2902, 3085
OliI CACNNNNGTG 1 cut(s) 2529
PaeI GCATGC 1 cut(s) 3180
PaeR7I CTCGAG 1 cut(s) 3491
PagI TCATGA 1 cut(s) 1093
PciSI GCTCTTC 1 cut(s) 114
PcsI WCGNNNNNNNCGW 3 cut(s) 21, 2904, 3082
PctI GAATGC 2 cut(s) 90, 1265
PdmI GAANNNNTTC 1 cut(s) 2423
PflFI GACNNNGTC 2 cut(s) 39, 539
PfoI TCCNGGA 1 cut(s) 526
Ple19I CGATCG 1 cut(s) 2590
PleI GAGTC 5 cut(s) 326, 517, 548, 721, 2963
PpsI GAGTC 5 cut(s) 326, 517, 548, 721, 2963
Ppu21I YACGTR 1 cut(s) 2510
Psp6I CCWGG 2 cut(s) 1439, 1567
PspFI CCCAGC 5 cut(s) 188, 2297, 2346, 2600, 3185
PspGI CCWGG 2 cut(s) 1439, 1567
PspN4I GGNNCC 7 cut(s) 1392, 1550, 1670, 2225, 2649, 2701, 3415
PspPI GGNCC 3 cut(s) 41, 1876, 3188
PstI CTGCAG 2 cut(s) 1618, 1936
PstNI CAGNNNCTG 1 cut(s) 959
PsuI RGATCY 7 cut(s) 9, 67, 904, 1506, 1699, 2647, 3487
PsyI GACNNNGTC 2 cut(s) 39, 539
PvuI CGATCG 1 cut(s) 2590
RgaI GCGATCGC 1 cut(s) 2590
RruI TCGCGA 1 cut(s) 2592
RsaI GTAC 8 cut(s) 20, 633, 678, 1312, 2508, 2623, 3195, 3447
RsaNI GTAC 8 cut(s) 19, 632, 677, 1311, 2507, 2622, 3194, 3446
RseI CAYNNNNRTG 3 cut(s) 858, 1005, 2529
SalI GTCGAC 2 cut(s) 647, 1903
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 7 cut(s) 660, 1121, 2211, 2277, 2637, 2886, 3288
Sau96I GGNCC 3 cut(s) 41, 1876, 3188
ScaI AGTACT 2 cut(s) 20, 1312
SchI GAGTC 5 cut(s) 327, 517, 549, 721, 2963
ScrFI CCNGG 3 cut(s) 528, 1441, 1569
SduI GDGCHC 3 cut(s) 1061, 1259, 1531
SexAI ACCWGGT 1 cut(s) 1439
SfaAI GCGATCGC 1 cut(s) 2590
SfcI CTRYAG 2 cut(s) 1614, 1932
Sfr274I CTCGAG 1 cut(s) 3491
SfuI TTCGAA 3 cut(s) 1716, 2902, 3085
SgfI GCGATCGC 1 cut(s) 2590
SinI GGWCC 1 cut(s) 41
SlaI CTCGAG 1 cut(s) 3491
SmiMI CAYNNNNRTG 3 cut(s) 858, 1005, 2529
SmlI CTYRAG 2 cut(s) 1322, 3491
SmoI CTYRAG 2 cut(s) 1322, 3491
SpeI ACTAGT 1 cut(s) 679
SphI GCATGC 1 cut(s) 3180
SspI AATATT 2 cut(s) 288, 1731
SspMI CTAG 4 cut(s) 680, 2795, 2940, 2957
StyD4I CCNGG 3 cut(s) 526, 1439, 1567
StyI CCWWGG 2 cut(s) 79, 1079
TaaI ACNGT 9 cut(s) 58, 1206, 1909, 2528, 2630, 2704, 2884, 2926, 3007
TaiI ACGT 3 cut(s) 2512, 2857, 2901
TaqII GACCGA 1 cut(s) 1464
TatI WGTACW 6 cut(s) 18, 631, 676, 1310, 2621, 3193
TauI GCSGC 2 cut(s) 3285, 3464
Tru1I TTAA 7 cut(s) 660, 1121, 2211, 2277, 2637, 2886, 3288
Tru9I TTAA 7 cut(s) 660, 1121, 2211, 2277, 2637, 2886, 3288
TscAI CASTG 6 cut(s) 229, 1061, 1189, 1914, 2545, 2869
TseFI GTSAC 5 cut(s) 224, 2089, 2522, 2536, 2554
Tsp45I GTSAC 5 cut(s) 224, 2089, 2522, 2536, 2554
TspDTI ATGAA 7 cut(s) 1150, 1701, 1710, 1831, 2079, 2262, 2355
TspGWI ACGGA 7 cut(s) 22, 259, 281, 398, 1036, 1661, 3517
TspRI CASTG 6 cut(s) 229, 1061, 1189, 1914, 2545, 2869
Tth111I GACNNNGTC 2 cut(s) 39, 539
VpaK11BI GGWCC 1 cut(s) 41
XagI CCTNNNNNAGG 1 cut(s) 1453
XapI RAATTY 2 cut(s) 2493, 2640
XbaI TCTAGA 1 cut(s) 2794
XceI RCATGY 2 cut(s) 2237, 3180
XcmI CCANNNNNNNNNTGG 2 cut(s) 880, 2980
XhoI CTCGAG 1 cut(s) 3491
XmiI GTMKAC 6 cut(s) 648, 783, 813, 1445, 1789, 1904
XmnI GAANNNNTTC 1 cut(s) 2423
XspI CTAG 4 cut(s) 680, 2795, 2940, 2957
ZrmI AGTACT 2 cut(s) 20, 1312
Zsp2I ATGCAT 2 cut(s) 2461, 3048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.