Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0007797

Physical Location & Seq

Genomic Coordinates Forward (+)

7001456 .. 7012680

Gene Structure 11225 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g12450.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3612 bp

ATGGCTCAGATCTTCGAGCACTTCGTCGTCTGCGGCATCGGGCCGGAGATTCGAACCCTCGACGGCGGCAGAGGATTCCACGGCTTCGCCGAAATGTACCTCCCGTCTTTGCTCGACCAGTTCCCTCCCGCCAACCACACTCTCTATCCTCCTCCGCCGCCTCAGCTCCCCACCTGTGTGCTGCCAGCTGGCGTTTCGTTCTACTCCTCGGGGTTTGATGCTAACGATGCGACGACGTTTCCGCGGAGCTATCCCATTGTTCTTACTGAGGGTGATGGTTCAAAAATCTATGTTAGTTGCATTGCTTTCCGGGATCCAGTGAGTGAGGATATTGCGGAAGCTTATTGTATACCGGCAAATTCATTTGCTGAAAAATGTATATGTCTAGTTTCGCGGTCACCTAGTCTCCGTTTACTTAAAAATGTACTCGAGGAGTTGTTTGCTCTCTGTTTTTCCTCTAGCGGAAGCAGCAAACCATTATGGGATATCATTGCATCTATGGTATCCAATGTACCTCTGCCTACCCCTGGAAAAGATCGAGTTTTGTTCGCTATCGACAATGTTCTGCTTTCTGTGGAGACTCCACCAAAGGATGGGCTCCCCCATGTTGATATATCGTTTCAGCCATTGGTACAGTGTCTAGATGTTGACAACTTGATTAAATTTTTCACAGCCGTGCTGCTTGAAAGAAGGATTTTGATCCGGTCACACAAATATTCCCTTTTGACCCTTGCATCAGAAGCTATTTGCTATTTAATTTATCCATTTCGGTGGCAGCAAGTCTACATTCCGTTACTCTTTTTCAGTGGAGTAGACTATATTGATGCCCCCACACCGTATATGATGGGTCTTCATTCAGGTGTTGATACATCTACTCTAGCAATGGATGGTGTAGTTATTGTGGACCTTGAGTTCAATCGTATCACAACAACAGAAGATATACCTTCTATTCCTGAGCCAGAACTAAGCTGTTTACGTCAGGATCTAACGCAGCTATTGTATCCCAATGTTGTGGGGATAGATCTGATGAAGGATGGTCTTTTCAATTCATCCGAGCAATATCCTAAACTTGGCAACAAACCTTGGGGAGAGGATCATGAACTACAACTTAGATTAGTGTTCTTAAAGTTCTTTGCATCGCTTTTAAGTGGTTATCGGAACTTCATAGAACTTTCTGCTACTCACGTCTTCAACGTTCAAGCATACTTGAAGATGCGCTCTCGTTCAACTCAGCAACCCCCAGAACCCATGCTGACACAATTTCTGGAATCACATGGTTTCATGGATTATCTGGAAAGAGGTTTGGGTTCTGATGAAAACAACAATAACCTACTTGACAAGTTACAAGATGCACTGGGAAGGGGCCAAAATCCTATTTCAATTCTTCCTTCAAATTCAGTGGAGCCTGAGATTATAACAATTGCAGATACAGATGTGGGAACATCAGGGACAGGTGGCACATACACTTATGACAGGTTTCCCTCAAACATCAGGACAGAAGAGGAAGAAGAAAAAAGGAAGCAAACTCTTGCTGCAGCAAGTGGTTTATCTGCTAGCGCACCTTCTGTCCTTACTGGTAAAGACCCCAAATCAGATCCATTGGAGCGGGCTGCTGAAAGGGAATGTATGGTTTTGGACATCAAAGTTAAGCTGCAGGGTCTGTGGCTGCGTCTTCTAAAACTGGGACCTACTGATGATCCTCTTTCTTCATTTGAGTATGGAACAATTCTTGCTTTAATTGAATCGGATGCAGAGGGGATTGGTGGCAGTGGGTTTGTTGAATGTATAAGGGAGCACATAAATTCGGGATGGCACTGTCAGTTAACTGAAGAACAATTTATTGCTGTGAAGGAACTGCTGAAAACAGCTATCAGCCGTGCAACCTCCAGAAATGATTGGATGACCATCAGAGACGCCCTTGAAGTGTCTGCTGAAATGTTCAAGAAGGATGGAAATAATGTTTCAGATTATGTTCAGCGTCATCTTATTTCTTTGTCCATCTGGGAGGAATTACGCTTTTGGGAAAGCTACTTTGACTGTTTAATGGAGCGTTCTGCAAACAAGTCGGCCAACTATGCCTCTTTGGTGACTGATCAGCTATCTTTGGTGGAATTACACATGGCCGGACTGGGACTTCCTGATCTTGATGCATGGTACATGATTGAAACAATAGCTGAGAAGAACAATATTGGATACACACAATTTATAAAGCTGAGGGGGTTGTTGTCACATGTTCAACAACTGCGGATTGGTTACTGGGGAATCAGTTCTTTCAAGGCCCAATCCATGTCTCATGGATTAGCATCTCCACGTTCAAAAGACGCCACAGTTGAGAGTCAGCAACCTGCCGAGGCCTCTGGTGTTGGGAGGAGCTGGGTTCAGAGCATGTTCAGCAGGGATAATTCAGCCAGACTCAACTCTTTCAGTCGTGTTCGCAAATGGACTGATGGTGGCTCTTCAGCTACAAATGAAAATGGGACATCCCGGAAGCGAGAAGTATCAGCAGCTGGACAGAAGAAAACCCAAACCAATGTTCGAGTACTCCGAGGTCATACTGGCCCAATTACTGCTCTACATTGTGTAACAAGGAGGGAAGTTTGGGATCTTGTGGGTGACCGTGAAGACGCAGGTTTGTTCATCAGTGGGAGCACAGACTGCACGGTTAAGATCTGGGATCCTAGTTTTCGTGGTAACGAACGTCGGGCAACTTTGAAAGGACACACAAGAACCATTCGTGCAATAAGTTCGGATCGAGGAAAAGTGGTATCTGGATCAGATGATCAATCTGTTTTAGTATGGGATAAGCAAACTACTCAGCTTCTGGAAGAGTTAAAAGGCCATGATGCACAGATTAGCTGTGCTCGCATGCTGTCAGGGGAACGTGTTCTTACTGCTGCTCACGATGGAACTGTTAAAATGTGGGATGTTAGAACTGATACTTGTGTGGCAACTGTTGGTCGTTGCTCTAGTGCCGTCCTATGCATGGAATACGATGACTCCACAGGAATCCTAGCTGCTGGTGGTAGAGATGCCATTGCTAACATCTGGGACATTCGCGCCGGTAGGCAAATGCACAAGCTTCTAGGGCACACAAAATGGATCCGGTCGATTAGAATGGTGGGGGATACAGTGGTTACTGGTAGTGATGATTGGACTGCAAGAATGTGGTCCATTTCCAGAGGAACATGTGATGCTGTTCTTGCATGCCATGCTGGTCCAATATTATCCGTTGAGTACTCCCAACTTGACAGAGGAATAATCACAGGTTCAACGGATGGCTTACTTCGCTTTTGGGAAAATGTAGAAGGAGGCATAAGATGTGTAAAAAATGTCACTGTTCATACTGCTGCTGTATTGTCCATCAATGCTGCGGAACATTGGTTAGGAATTGGAGCAGCTGATAATTCCATGTCTCTTTTTCATCGGCCTCAAGAGAAACTTGGCGGCTTTTCTAATACGGGATCAAAAATGGCTGGGTGGCAACTATACCGAACACCACAAAGAACAGCTGCTGTGGTAAGATGCGTTGCTACAGACCTCGAGAGAAAAAGGATATGCAGTGGTGACCGCAAGGGCCAGCTTAGGCTGTGGGAAGCCACTATAAACATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0002682 GO:0002683 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0007049 GO:0007275 GO:0008150 GO:0009791 GO:0009825 GO:0009888 GO:0009987 GO:0010235 GO:0010374 GO:0010440 GO:0016020 GO:0016049 GO:0022402 GO:0030135 GO:0030136 GO:0031347 GO:0031348 GO:0031410 GO:0031461 GO:0031982 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045824 GO:0048519 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0051301 GO:0061640 GO:0065007 GO:0071944 GO:0080008 GO:0080134 GO:0090558 GO:0097708 GO:1902410 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DENN WD40 dDENN uDENN

Protein Analysis

1204

Amino Acids

133.32

Weight (kDa)

5.75

Isoelectric Point (pI)

41.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
uDENN	PF03456	35 - 103	4.2e-12	uDENN domain
DENN	PF02141	123 - 304	9.1e-43	DENN (AEX-3) domain
Beta-prop_WDR3_2nd	PF25172	874 - 975	1e-09	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	876 - 1027	7.2e-27	WDR5 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	876 - 939	3.7e-06	TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	876 - 1041	1.4e-07	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	877 - 1026	1.4e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	879 - 1099	1.8e-31	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	879 - 985	1.8e-09	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	879 - 981	3.9e-07	WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	880 - 1010	8.8e-07	MABP1/WDR62 first WD40 domain
WDR55	PF24796	880 - 1097	4.9e-19	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	896 - 1098	3.2e-14	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	898 - 985	1.2e-17	WDR3 first beta-propeller domain
EIF3I	PF24805	903 - 998	5.5e-07	EIF3I
Beta-prop_WDR41	PF25178	903 - 1010	4.8e-09	WDR41
WD40_Prp19	PF24814	916 - 992	1e-10	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	918 - 1063	1.7e-14	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	941 - 1074	1.3e-10	TEP-1 second beta-propeller
WD40_Gbeta	PF25391	944 - 1140	1.8e-15	G protein beta WD-40 repeat protein
Beta-prop_DCAF4	PF23761	947 - 1023	6.3e-07	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	960 - 1062	2.6e-07	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	984 - 1065	5.9e-07	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	988 - 1147	9.6e-11	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	989 - 1122	2e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	990 - 1099	1.7e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	991 - 1112	1.3e-14	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	1003 - 1199	7.1e-13	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	1041 - 1114	8e-07	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007797)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49040 AT1G49040 AT1G49040 AT1G49040
fragaria_vesca	FvH4_5g12450 FvH4_5g12450
malus_domestica	MD14G1224200.v1.1
prunus_persica	Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1
pyrus_communis	pycom06g19080 pycom14g18580
rosa_chinensis	RchiOBHm_Chr7g0185021
rosa_laevigata	RLG00000004938
rosa_multiflora	Rmu_ssc0000289.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269260
rosa_rugosa	Rorug06G0466200
rosa_samantha	Rh7AG076700 Rh7BG070700 Rh7CG072200 Rh7DG071200
rosa_wichuraiana	Rw7G005890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1415, 2207
Acc36I	ACCTGC	2 cut(s)	2353, 2618
AccB7I	CCANNNNNTGG	1 cut(s)	593
AccBSI	CCGCTC	1 cut(s)	1606
AccI	GTMKAC	3 cut(s)	349, 783, 813
AccII	CGCG	3 cut(s)	244, 394, 3057
AclI	AACGTT	1 cut(s)	1194
AcoI	YGGCCR	2 cut(s)	2067, 2121
AcsI	RAATTY	4 cut(s)	358, 662, 1393, 1801
AcuI	CTGAAG	2 cut(s)	1848, 2442
AcyI	GRCGYC	2 cut(s)	1914, 2322
AfaI	GTAC	7 cut(s)	98, 426, 513, 633, 2156, 2541, 3236
AfiI	CCNNNNNNNGG	4 cut(s)	527, 593, 1070, 2983
AflIII	ACRYGT	3 cut(s)	2230, 2881, 3185
AjiI	CACGTC	1 cut(s)	1186
AjnI	CCWGG	1 cut(s)	526
AjuI	GAANNNNNNNTTGG	2 cut(s)	2517, 2549
AleI	CACNNNNGTG	2 cut(s)	176, 674
AloI	GAACNNNNNNTCC	2 cut(s)	896, 928
Alw21I	GWGCWC	4 cut(s)	21, 1797, 2651, 2863
Alw26I	GTCTC	5 cut(s)	410, 572, 1905, 2295, 3417
AlwNI	CAGNNNCTG	4 cut(s)	1660, 2507, 2821, 3512
Ama87I	CYCGRG	3 cut(s)	208, 428, 3539
ApoI	RAATTY	4 cut(s)	358, 662, 1393, 1801
Asp700I	GAANNNNTTC	2 cut(s)	2266, 2883
AspLEI	GCGC	3 cut(s)	1218, 1559, 3059
AspS9I	GGNCC	9 cut(s)	41, 904, 1363, 1685, 2278, 2558, 3168, 3215, 3574
AsuC2I	CCSGG	2 cut(s)	311, 2485
AsuHPI	GGTGA	5 cut(s)	284, 390, 2098, 2624, 3575
AsuII	TTCGAA	1 cut(s)	52
AsuNHI	GCTAGC	1 cut(s)	1553
AvaI	CYCGRG	3 cut(s)	208, 428, 3539
AvaII	GGWCC	4 cut(s)	904, 1685, 3168, 3215
BaeGI	GKGCMC	1 cut(s)	3090
BaeI	ACNNNNGTAYC	6 cut(s)	1419, 1452, 2928, 2961, 3117, 3150
BamHI	GGATCC	3 cut(s)	313, 2674, 3099
BanII	GRGCYC	1 cut(s)	600
BbsI	GAAGAC	4 cut(s)	842, 1180, 1664, 2628
Bbv12I	GWGCWC	4 cut(s)	21, 1797, 2651, 2863
BbvCI	CCTCAGC	2 cut(s)	162, 2213
BceAI	ACGGC	5 cut(s)	79, 97, 659, 1860, 2957
BcgI	CGANNNNNNTGC	4 cut(s)	2046, 2080, 2727, 2761
BciT130I	CCWGG	1 cut(s)	528
BciVI	GTATCC	4 cut(s)	514, 1011, 2186, 3118
BclI	TGATCA	2 cut(s)	2092, 2779
BcnI	CCSGG	2 cut(s)	311, 2485
BcoDI	GTCTC	5 cut(s)	410, 572, 1905, 2295, 3417
BfmI	CTRYAG	3 cut(s)	1533, 1652, 3531
BfuAI	ACCTGC	2 cut(s)	2353, 2618
BfuI	GTATCC	4 cut(s)	514, 1011, 2186, 3118
BglII	AGATCT	3 cut(s)	9, 1021, 2667
BmcAI	AGTACT	2 cut(s)	2541, 3236
Bme1390I	CCNGG	3 cut(s)	311, 528, 2485
Bme18I	GGWCC	4 cut(s)	904, 1685, 3168, 3215
BmeT110I	CYCGRG	3 cut(s)	208, 428, 3539
BmgBI	CACGTC	1 cut(s)	1186
BmgT120I	GGNCC	9 cut(s)	41, 904, 1363, 1685, 2278, 2558, 3168, 3215, 3574
BmiI	GGNNCC	7 cut(s)	315, 599, 1364, 1404, 1686, 2676, 3101
BmrFI	CCNGG	3 cut(s)	311, 528, 2485
BmrI	ACTGGG	4 cut(s)	1364, 1691, 2138, 2266
BmtI	GCTAGC	1 cut(s)	1557
BmuI	ACTGGG	4 cut(s)	1364, 1691, 2138, 2266
BpiI	GAAGAC	4 cut(s)	842, 1180, 1664, 2628
BpmI	CTGGAG	1 cut(s)	1870
Bpu10I	CCTNAGC	4 cut(s)	162, 954, 2213, 3581
Bpu14I	TTCGAA	1 cut(s)	52
BpuEI	CTTGAG	2 cut(s)	929, 3414
BpuMI	CCSGG	2 cut(s)	311, 2485
BsaBI	GATNNNNATC	3 cut(s)	698, 1904, 2302
BsaHI	GRCGYC	2 cut(s)	1914, 2322
BsaJI	CCNNGG	7 cut(s)	79, 207, 242, 526, 1082, 2349, 2545
BsaWI	WCCGGW	2 cut(s)	702, 3102
BsaXI	ACNNNNNCTCC	4 cut(s)	390, 420, 2981, 3011
Bsc4I	CCNNNNNNNGG	4 cut(s)	527, 593, 1070, 2983
Bse118I	RCCGGY	2 cut(s)	352, 3059
Bse1I	ACTGG	9 cut(s)	118, 317, 1359, 1579, 1686, 2133, 2261, 2560, 3142
Bse3DI	GCAATG	4 cut(s)	300, 489, 888, 3033
Bse8I	GATNNNNATC	3 cut(s)	698, 1904, 2302
BseBI	CCWGG	1 cut(s)	528
BseDI	CCNNGG	7 cut(s)	79, 207, 242, 526, 1082, 2349, 2545
BseJI	GATNNNNATC	3 cut(s)	698, 1904, 2302
BseLI	CCNNNNNNNGG	4 cut(s)	527, 593, 1070, 2983
BseMI	GCAATG	4 cut(s)	300, 489, 888, 3033
BseMII	CTCAG	9 cut(s)	20, 176, 258, 945, 1244, 1398, 2166, 2204, 2828
BseNI	ACTGG	9 cut(s)	118, 317, 1359, 1579, 1686, 2133, 2261, 2560, 3142
BseRI	GAGGAG	4 cut(s)	141, 196, 446, 2383
BseSI	GKGCMC	1 cut(s)	3090
BseYI	CCCAGC	2 cut(s)	2373, 3473
BsgI	GTGCAG	1 cut(s)	2641
Bsh1236I	CGCG	3 cut(s)	244, 394, 3057
Bsh1285I	CGRYCG	1 cut(s)	3107
BsiEI	CGRYCG	1 cut(s)	3107
BsiHKAI	GWGCWC	4 cut(s)	21, 1797, 2651, 2863
BsiHKCI	CYCGRG	3 cut(s)	208, 428, 3539
BsiSI	CCGG	8 cut(s)	44, 310, 353, 703, 2124, 2485, 3060, 3103
BslFI	GGGAC	5 cut(s)	1462, 1698, 2145, 2491, 3062
BslI	CCNNNNNNNGG	4 cut(s)	527, 593, 1070, 2983
BsmAI	GTCTC	5 cut(s)	410, 572, 1905, 2295, 3417
BsmBI	CGTCTC	1 cut(s)	1905
BsmFI	GGGAC	5 cut(s)	1462, 1698, 2145, 2491, 3062
BsoBI	CYCGRG	3 cut(s)	208, 428, 3539
Bsp119I	TTCGAA	1 cut(s)	52
Bsp1286I	GDGCHC	6 cut(s)	21, 600, 1797, 2651, 2863, 3090
BspCNI	CTCAG	9 cut(s)	19, 175, 259, 946, 1243, 1399, 2167, 2205, 2827
BspFNI	CGCG	3 cut(s)	244, 394, 3057
BspHI	TCATGA	1 cut(s)	1096
BspLI	GGNNCC	7 cut(s)	315, 599, 1364, 1404, 1686, 2676, 3101
BspMAI	CTGCAG	2 cut(s)	1537, 1656
BspMI	ACCTGC	2 cut(s)	2353, 2618
BspOI	GCTAGC	1 cut(s)	1557
BspQI	GCTCTTC	1 cut(s)	2461
BspT104I	TTCGAA	1 cut(s)	52
BsrBI	CCGCTC	1 cut(s)	1606
BsrDI	GCAATG	4 cut(s)	300, 489, 888, 3033
BsrFI	RCCGGY	2 cut(s)	352, 3059
BsrI	ACTGG	9 cut(s)	118, 317, 1359, 1579, 1686, 2133, 2261, 2560, 3142
BssAI	RCCGGY	2 cut(s)	352, 3059
BssECI	CCNNGG	7 cut(s)	79, 207, 242, 526, 1082, 2349, 2545
BssNAI	GTATAC	1 cut(s)	350
BssNI	GRCGYC	2 cut(s)	1914, 2322
BssT1I	CCWWGG	1 cut(s)	1082
Bst1107I	GTATAC	1 cut(s)	350
Bst2UI	CCWGG	1 cut(s)	528
Bst6I	CTCTTC	3 cut(s)	1494, 2461, 2820
BstACI	GRCGYC	2 cut(s)	1914, 2322
BstAPI	GCANNNNNTGC	2 cut(s)	2655, 3209
BstBI	TTCGAA	1 cut(s)	52
BstC8I	GCNNGC	8 cut(s)	186, 190, 1555, 1608, 2863, 2867, 3205, 3578
BstDSI	CCRYGG	2 cut(s)	79, 242
BstEII	GGTNACC	3 cut(s)	396, 2612, 3563
BstFNI	CGCG	3 cut(s)	244, 394, 3057
BstHHI	GCGC	3 cut(s)	1218, 1559, 3059
BstMAI	GTCTC	5 cut(s)	410, 572, 1905, 2295, 3417
BstMCI	CGRYCG	1 cut(s)	3107
BstNI	CCWGG	1 cut(s)	528
BstNSI	RCATGY	5 cut(s)	2234, 2389, 2869, 3189, 3207
BstPI	GGTNACC	3 cut(s)	396, 2612, 3563
BstSCI	CCNGG	3 cut(s)	309, 526, 2483
BstSFI	CTRYAG	3 cut(s)	1533, 1652, 3531
BstSLI	GKGCMC	1 cut(s)	3090
BstUI	CGCG	3 cut(s)	244, 394, 3057
BstV2I	GAAGAC	4 cut(s)	842, 1180, 1664, 2628
BstX2I	RGATCY	9 cut(s)	9, 313, 982, 1021, 1594, 2602, 2667, 2674, 3099
BstXI	CCANNNNNNTGG	2 cut(s)	771, 1012
BstYI	RGATCY	9 cut(s)	9, 313, 982, 1021, 1594, 2602, 2667, 2674, 3099
BstZ17I	GTATAC	1 cut(s)	350
BsuI	GTATCC	4 cut(s)	514, 1011, 2186, 3118
BtgI	CCRYGG	2 cut(s)	79, 242
BtgZI	GCGATG	1 cut(s)	1122
BtrI	CACGTC	1 cut(s)	1186
BtsI	GCAGTG	2 cut(s)	1774, 3565
BveI	ACCTGC	2 cut(s)	2353, 2618
Cac8I	GCNNGC	8 cut(s)	186, 190, 1555, 1608, 2863, 2867, 3205, 3578
CaiI	CAGNNNCTG	4 cut(s)	1660, 2507, 2821, 3512
CciI	TCATGA	1 cut(s)	1096
CfoI	GCGC	3 cut(s)	1218, 1559, 3059
Cfr10I	RCCGGY	2 cut(s)	352, 3059
Cfr13I	GGNCC	9 cut(s)	41, 904, 1363, 1685, 2278, 2558, 3168, 3215, 3574
Cfr42I	CCGCGG	1 cut(s)	245
CseI	GACGC	5 cut(s)	1658, 1922, 1967, 2330, 2633
Csp6I	GTAC	7 cut(s)	97, 425, 512, 632, 2155, 2540, 3235
CspCI	CAANNNNNGTGG	2 cut(s)	1380, 1415
CviQI	GTAC	7 cut(s)	97, 425, 512, 632, 2155, 2540, 3235
EaeI	YGGCCR	2 cut(s)	2067, 2121
Eam1104I	CTCTTC	3 cut(s)	1494, 2461, 2820
EarI	CTCTTC	3 cut(s)	1494, 2461, 2820
EciI	GGCGGA	1 cut(s)	144
Eco130I	CCWWGG	1 cut(s)	1082
Eco147I	AGGCCT	1 cut(s)	2354
Eco24I	GRGCYC	1 cut(s)	600
Eco32I	GATATC	1 cut(s)	487
Eco47I	GGWCC	4 cut(s)	904, 1685, 3168, 3215
Eco57I	CTGAAG	2 cut(s)	1848, 2442
Eco88I	CYCGRG	3 cut(s)	208, 428, 3539
Eco91I	GGTNACC	3 cut(s)	396, 2612, 3563
EcoO109I	RGGNCCY	1 cut(s)	1685
EcoO65I	GGTNACC	3 cut(s)	396, 2612, 3563
EcoRII	CCWGG	1 cut(s)	526
EcoRV	GATATC	1 cut(s)	487
EcoT14I	CCWWGG	1 cut(s)	1082
EcoT22I	ATGCAT	2 cut(s)	2152, 2984
EcoT38I	GRGCYC	1 cut(s)	600
ErhI	CCWWGG	1 cut(s)	1082
Esp3I	CGTCTC	1 cut(s)	1905
FalI	AAGNNNNNCTT	2 cut(s)	3423, 3455
FaqI	GGGAC	5 cut(s)	1462, 1698, 2145, 2491, 3062
FauI	CCCGC	2 cut(s)	136, 1599
FbaI	TGATCA	2 cut(s)	2092, 2779
FblI	GTMKAC	3 cut(s)	349, 783, 813
FriOI	GRGCYC	1 cut(s)	600
GlaI	GCGC	3 cut(s)	1217, 1558, 3058
GsaI	CCCAGC	2 cut(s)	2377, 3477
GsuI	CTGGAG	1 cut(s)	1870
HapII	CCGG	8 cut(s)	44, 310, 353, 703, 2124, 2485, 3060, 3103
HgaI	GACGC	5 cut(s)	1658, 1922, 1967, 2330, 2633
HhaI	GCGC	3 cut(s)	1218, 1559, 3059
Hin1I	GRCGYC	2 cut(s)	1914, 2322
Hin6I	GCGC	3 cut(s)	1216, 1557, 3057
HinP1I	GCGC	3 cut(s)	1216, 1557, 3057
HincII	GTYRAC	2 cut(s)	649, 1824
HindII	GTYRAC	2 cut(s)	649, 1824
HindIII	AAGCTT	2 cut(s)	339, 3077
HpaI	GTTAAC	1 cut(s)	1824
HpaII	CCGG	8 cut(s)	44, 310, 353, 703, 2124, 2485, 3060, 3103
HphI	GGTGA	5 cut(s)	284, 390, 2098, 2624, 3575
Hpy166II	GTNNAC	8 cut(s)	350, 413, 649, 784, 814, 904, 974, 1824
Hpy8I	GTNNAC	8 cut(s)	350, 413, 649, 784, 814, 904, 974, 1824
Hpy99I	CGWCG	5 cut(s)	29, 65, 235, 238, 2703
HpyAV	CCTTC	9 cut(s)	684, 954, 1024, 1353, 1398, 1572, 1843, 1939, 3299
HpyCH4IV	ACGT	7 cut(s)	236, 976, 1185, 1194, 2311, 2698, 2881
HpySE526I	ACGT	7 cut(s)	236, 976, 1185, 1194, 2311, 2698, 2881
Hsp92I	GRCGYC	2 cut(s)	1914, 2322
HspAI	GCGC	3 cut(s)	1216, 1557, 3057
Ksp22I	TGATCA	2 cut(s)	2092, 2779
KspAI	GTTAAC	1 cut(s)	1824
KspI	CCGCGG	1 cut(s)	245
LguI	GCTCTTC	1 cut(s)	2461
MaeII	ACGT	7 cut(s)	236, 976, 1185, 1194, 2311, 2698, 2881
MbiI	CCGCTC	1 cut(s)	1606
MfeI	CAATTG	1 cut(s)	1419
MflI	RGATCY	9 cut(s)	9, 313, 982, 1021, 1594, 2602, 2667, 2674, 3099
MhlI	GDGCHC	6 cut(s)	21, 600, 1797, 2651, 2863, 3090
MlyI	GAGTC	4 cut(s)	574, 2344, 2406, 2990
Mph1103I	ATGCAT	2 cut(s)	2152, 2984
MroXI	GAANNNNTTC	2 cut(s)	2266, 2883
MslI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 858
MspA1I	CMGCKG	5 cut(s)	188, 244, 2507, 3398, 3509
MspI	CCGG	8 cut(s)	44, 310, 353, 703, 2124, 2485, 3060, 3103
MspR9I	CCNGG	3 cut(s)	311, 528, 2485
MunI	CAATTG	1 cut(s)	1419
MvaI	CCWGG	1 cut(s)	528
MvnI	CGCG	3 cut(s)	244, 394, 3057
NciI	CCSGG	2 cut(s)	311, 2485
NheI	GCTAGC	1 cut(s)	1553
NlaIV	GGNNCC	7 cut(s)	315, 599, 1364, 1404, 1686, 2676, 3101
NmeAIII	GCCGAG	1 cut(s)	2374
NmuCI	GTSAC	7 cut(s)	396, 705, 2086, 2226, 2612, 3331, 3563
NsiI	ATGCAT	2 cut(s)	2152, 2984
NspI	RCATGY	5 cut(s)	2234, 2389, 2869, 3189, 3207
NspV	TTCGAA	1 cut(s)	52
OliI	CACNNNNGTG	2 cut(s)	176, 674
PaeI	GCATGC	2 cut(s)	2869, 3207
PaeR7I	CTCGAG	2 cut(s)	428, 3539
PagI	TCATGA	1 cut(s)	1096
PceI	AGGCCT	1 cut(s)	2354
PciI	ACATGT	2 cut(s)	2230, 3185
PciSI	GCTCTTC	1 cut(s)	2461
PcsI	WCGNNNNNNNCGW	4 cut(s)	21, 87, 1191, 2542
PdmI	GAANNNNTTC	2 cut(s)	2266, 2883
PfeI	GAWTC	6 cut(s)	49, 75, 1268, 1742, 2262, 3006
PflMI	CCANNNNNTGG	1 cut(s)	593
PfoI	TCCNGGA	2 cut(s)	309, 2483
PleI	GAGTC	4 cut(s)	574, 2343, 2406, 2990
PpsI	GAGTC	4 cut(s)	574, 2343, 2406, 2990
PpuMI	RGGWCCY	1 cut(s)	1685
PscI	ACATGT	2 cut(s)	2230, 3185
PsiI	TTATAA	2 cut(s)	1415, 2207
Psp1406I	AACGTT	1 cut(s)	1194
Psp5II	RGGWCCY	1 cut(s)	1685
Psp6I	CCWGG	1 cut(s)	526
PspEI	GGTNACC	3 cut(s)	396, 2612, 3563
PspFI	CCCAGC	2 cut(s)	2373, 3473
PspGI	CCWGG	1 cut(s)	526
PspN4I	GGNNCC	7 cut(s)	315, 599, 1364, 1404, 1686, 2676, 3101
PspPI	GGNCC	9 cut(s)	41, 904, 1363, 1685, 2278, 2558, 3168, 3215, 3574
PspPPI	RGGWCCY	1 cut(s)	1685
PspXI	VCTCGAGB	1 cut(s)	428
PstI	CTGCAG	2 cut(s)	1537, 1656
PstNI	CAGNNNCTG	4 cut(s)	1660, 2507, 2821, 3512
PsuI	RGATCY	9 cut(s)	9, 313, 982, 1021, 1594, 2602, 2667, 2674, 3099
PvuII	CAGCTG	4 cut(s)	188, 2507, 3398, 3509
RsaI	GTAC	7 cut(s)	98, 426, 513, 633, 2156, 2541, 3236
RsaNI	GTAC	7 cut(s)	97, 425, 512, 632, 2155, 2540, 3235
RseI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 858
SacII	CCGCGG	1 cut(s)	245
SapI	GCTCTTC	1 cut(s)	2461
Sau96I	GGNCC	9 cut(s)	41, 904, 1363, 1685, 2278, 2558, 3168, 3215, 3574
ScaI	AGTACT	2 cut(s)	2541, 3236
SchI	GAGTC	4 cut(s)	574, 2344, 2406, 2990
ScrFI	CCNGG	3 cut(s)	311, 528, 2485
SduI	GDGCHC	6 cut(s)	21, 600, 1797, 2651, 2863, 3090
SfcI	CTRYAG	3 cut(s)	1533, 1652, 3531
Sfr274I	CTCGAG	2 cut(s)	428, 3539
Sfr303I	CCGCGG	1 cut(s)	245
SfuI	TTCGAA	1 cut(s)	52
SgrBI	CCGCGG	1 cut(s)	245
SinI	GGWCC	4 cut(s)	904, 1685, 3168, 3215
SlaI	CTCGAG	2 cut(s)	428, 3539
SmiMI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 858
SmlI	CTYRAG	4 cut(s)	428, 908, 3429, 3539
SmoI	CTYRAG	4 cut(s)	428, 908, 3429, 3539
SphI	GCATGC	2 cut(s)	2869, 3207
SseBI	AGGCCT	1 cut(s)	2354
SspI	AATATT	3 cut(s)	716, 2188, 3222
StuI	AGGCCT	1 cut(s)	2354
StyD4I	CCNGG	3 cut(s)	309, 526, 2483
StyI	CCWWGG	1 cut(s)	1082
TaiI	ACGT	7 cut(s)	239, 979, 1188, 1197, 2314, 2701, 2884
TatI	WGTACW	3 cut(s)	424, 2539, 3234
TauI	GCSGC	4 cut(s)	36, 69, 160, 3447
TfiI	GAWTC	6 cut(s)	49, 75, 1268, 1742, 2262, 3006
TseFI	GTSAC	7 cut(s)	396, 705, 2086, 2226, 2612, 3331, 3563
Tsp45I	GTSAC	7 cut(s)	396, 705, 2086, 2226, 2612, 3331, 3563
TspGWI	ACGGA	4 cut(s)	398, 780, 3217, 3287
Van91I	CCANNNNNTGG	1 cut(s)	593
VpaK11BI	GGWCC	4 cut(s)	904, 1685, 3168, 3215
XapI	RAATTY	4 cut(s)	358, 662, 1393, 1801
XbaI	TCTAGA	1 cut(s)	640
XceI	RCATGY	5 cut(s)	2234, 2389, 2869, 3189, 3207
XhoI	CTCGAG	2 cut(s)	428, 3539
XmiI	GTMKAC	3 cut(s)	349, 783, 813
XmnI	GAANNNNTTC	2 cut(s)	2266, 2883
ZrmI	AGTACT	2 cut(s)	2541, 3236
Zsp2I	ATGCAT	2 cut(s)	2152, 2984

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.