Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0007797

Physical Location & Seq

Genomic Coordinates Forward (+)

5488508 .. 5500854

Gene Structure 12347 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG076700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3126 bp

ATGGTATCCAATGTACCTCTGCCTACTCCTGGAAAAGATCGAGTTTTGTTTGCTATTGACAATTGTCTGCTTTCTGTGGAGACTCCACCGAAGGATGGGCTCCCTTATGTTGATATATCATTTCAGCCGTTGGTACAGTGTCTAGATGTTGACAACTTAATTAAATTTTTCACAGCCGTGCTGCTTGAAAGGAGGATTTTGATCCGATCACACAAATATTCACTTTTGACCCTTGCATCAGAAGCCATTTGCTATTTAATTTATCCATTTCGGTGGCAGCAAGTCTACATTCCGTTACTCTTTTTCAGTGGAGTAGACTATATTGATGCCCCTACGCCGTATATGATGGGTCTTCATTCTGGTGTTGATACATCTACTCTAGCAATGGATGGTGTAGTTATTGTGGACCTTGAGTTCAATCGTATCACAACAACAGAAGATATACCTTCTATTCCTGAGCCAGAACTAAGCTGTTTGCGTCAGGAAATAACGCAGCTATTGTATCCCAATGTTGTGGGGATAGATCTGATGAAGGATGCTCTTTTCAGTTCATCCGAGCAATATCCTAAACTTGGCAACAAACCTTGGGGAGAGGATCATGACCTACAACTTAGGCTAGTGTTCTTAAAGTTCTTTGCATCACTTTTAAGTGGCTATCGGAACTTCATAGAACTTTCTGCTACTCACGTCTTCAATGTTCAAGCATTCTTGAAGATGCGCTCTCGGTCAACTCAGCAACCCCCAGAACCCATGTTGACACAATTTTTGGAATCACATGGTTTCATGGATTATCTGGAAAGAGGTTTGGGTTCTGATGAAAACAACAATAATCTACTTGACAAGTTACAAGATGCACTGGGAAGGGGACAAAATCCTATTTCAATTCTTCCTTCAAATTCAGTTGAGCCTGAGATTATAACAATTGCGGATACAGATGTGGGAACATCAGGGTCAGGTGGCACATACACTTATGACAGGTTTCCCTCAAACATTAGGACAGAAGAGGAAGAAGAAAAGAGGAAGCAAATTCTTGCTGCAGCAAGTGGTTTATCTGCTAGCTCACCTTCTGTCCTTCCTGGTAAAGACCCCAAATCTGAAACTCTAACTCCATTGGAGCGGGCTGCTGAAAGGGAATGTATGGTTTTGGACATAAAAGTTAAGCTGCAGGGTCTGTGGCTGCGTCTTCTAAAACTGGGACCTACTGATGATCCTCTCTCTTCATTTGAATATGGAACAATCCTTGCTTTAATTGAATCTGATGCAGAAGGAATTGGTGGCAGTGGGTTTGTTGAATGTATAAGGGAGCACATAAATTCGGGATGGCACTGTCAGTTGACTGAAGAACAATTCATCGCTGTGAAGGAACTGCTGAAAACAGCTATCAGTCGTGCAACGTCCAGAAATGATTGGATGACCATCAGAGATGCCCTTGAAGTGTCTGCTGAAATGTTTAAGAAGGATGGAAATAATGTTTCAGATTATGTTCAGCGTCATCTTATATCTTTGTCCATTTGGGAGGAATTACGTTTCTGGGAAAGCTACTTTGACTGTCTAATGGAACGGTCTTCAAACAAGTCGGCCAACTATGCCTCTTTGGTGACTGATCAGCTATCTTTGGTGGAATTACACATGGCTGGACTAGGACTTCCTGATCTTGATGCATGGTACATGATTGAAACAATAGCTGAGAAGAACAACATTGGATACACACAATTTATAAAGCTGAGGGGGTTATTGTCGCATGTTCAACAACTACGAATTGGTTACTGGGGAATCAGTTCTTTCAAGGCCCAATCCATGTCTCATGGATTAGCATCTCCACGTTCAAAAGATGCCGCAGTTGAGAGTCAGCAACCTGCCGAGGCCTCCGGTGTTGGGAGGAGCTGGGTTCAGAGCATGTTCAGCAGGGATAATTCAACCAGATTAAACTCTTTCAGTCGTGTTCGCAAATGGACTTCTGATGGTGGCTCTTCAGCTACAAATGAAAATGGGACATCTCGTAAACGAGAATTATCTGCAGCTGGACAGAAGAAAACCCAAACCAATGTTCGAGTACTTAGAGGCCATACTGGTCCTATCACTGCTCTACATTGTGTAACAAGGAGGGAAGTTTGGGATCTAGTGGGTGACCGTGAAGACGCAGGTTTTTTCATCAGTGGGAGCACAGACTGCACAGTTAAGATCTGGGATCCTAGTTTTCGTGGTAACGAACTCCGGGCAACCCTGAAAGGACACACAAGAACCATCCGTGCAATAAGTTCGGATCGAGGAAAGGTGGTATCTGGATCAGATGATCAATCTGTTTTAGTATGGGATAAGCAAACTACTCAGCTTCTAGAAGAGTTGAAAGGCCATGATGCACAGATTAGCTGTGCTCGCATGCTGTCAGGGGAACGTGTCCTTACTGCTGCTCATGATGGAACTGTTAAAATGTGGGATGTTAGAACTGATACTTGTGTGGCAACTGTTGGTCGTTGCTCTAGTGCCGTCCTATGCATGGAATATGATGACTCCACAGGAATCCTAGCTGCTGGTGGTAGAGATGCCATTGCTAACATTTGGGACATTCGTGCCGGTAGGCAAATGCACAAGCTTCTAGGGCACACAAAATGGATCCGGTCAATTAGAATGGTGGGGGACACAGTGGTTACTGGTAGTGACGATTGGACTGCAAGAATGTGGTCCATTTCCAGAGGAACATGTGATGCTGTTCTTGCATGCCATGCTGGCCCAATATTATCCGTTGAGTACTCCACACTTGACAGAGGAATAATCACAGGTTCAACGGATGGCTTACTTCGCTTTTGGGAAAATGTGGAAGGAGGTATAAGATGTGTAAAAAATGTTACTGTTCACACTGCTGCTATATTGTCCATCAATGCTGCGGAACATTGGTTAGGAATCGGAGCAGCTGATAATTCCATGTCTCTTTTTCATCGGCCTCAAGAGAAACTTGGCGGCTTCTCTAATACGGGATCAAAAATGGCTGGGTGGCAACTATACCGAACACCACAAAGAACAGCTGCTGTGGTAAGATGCGTTGCTACAGACCTTGAGAGAAAAAGGATATGCAGTGGTGATCGCAAGGGCCAGCTTCGGCTATGGGAAGCCACCATAAACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0002682 GO:0002683 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0007049 GO:0007275 GO:0008150 GO:0009791 GO:0009825 GO:0009888 GO:0009987 GO:0010235 GO:0010374 GO:0010440 GO:0016020 GO:0016049 GO:0022402 GO:0030135 GO:0030136 GO:0031347 GO:0031348 GO:0031410 GO:0031461 GO:0031982 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045824 GO:0048519 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0051301 GO:0061640 GO:0065007 GO:0071944 GO:0080008 GO:0080134 GO:0090558 GO:0097708 GO:1902410 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DENN WD40 dDENN uDENN

Protein Analysis

1041

Amino Acids

115.74

Weight (kDa)

5.9

Isoelectric Point (pI)

40.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DENN	PF02141	2 - 138	1.6e-34	DENN (AEX-3) domain
Beta-prop_WDR5	PF25175	713 - 861	1e-26	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	713 - 813	6e-09	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	714 - 879	2.4e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st	PF25173	716 - 823	1.9e-21	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	716 - 864	3.5e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	716 - 912	1.3e-16	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	717 - 952	6.2e-31	THOC3 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	718 - 848	1.7e-06	MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st	PF24817	718 - 822	3.8e-09	WDHD1 first WD40 domain
WDR55	PF24796	719 - 935	2.4e-19	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	733 - 936	1.6e-14	WDR36/Utp21 first beta-propeller
Beta-prop_WDR41	PF25178	741 - 849	1.4e-09	WDR41
EIF3I	PF24805	741 - 835	8e-07	EIF3I
WD40_Prp19	PF24814	754 - 829	2e-10	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	758 - 901	2e-14	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	781 - 978	8.4e-16	G protein beta WD-40 repeat protein
Beta-prop_DCAF4	PF23761	785 - 861	5.2e-07	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	798 - 900	1.7e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	798 - 878	1.2e-13	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	826 - 985	1.5e-10	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	826 - 960	2.6e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	828 - 937	2.2e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	829 - 949	9.8e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	842 - 1037	2e-12	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	879 - 952	5.5e-07	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007797)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49040 AT1G49040 AT1G49040 AT1G49040
fragaria_vesca	FvH4_5g12450 FvH4_5g12450
malus_domestica	MD14G1224200.v1.1
prunus_persica	Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1
pyrus_communis	pycom06g19080 pycom14g18580
rosa_chinensis	RchiOBHm_Chr7g0185021
rosa_laevigata	RLG00000004938
rosa_multiflora	Rmu_ssc0000289.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269260
rosa_rugosa	Rorug06G0466200
rosa_samantha	Rh7AG076700 Rh7BG070700 Rh7CG072200 Rh7DG071200
rosa_wichuraiana	Rw7G005890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	917, 1718
Acc36I	ACCTGC	2 cut(s)	1864, 2132
AccBSI	CCGCTC	1 cut(s)	1117
AccI	GTMKAC	2 cut(s)	285, 315
AciI	CCGC	5 cut(s)	926, 1117, 1836, 2885, 2958
AcoI	YGGCCR	1 cut(s)	1578
AcsI	RAATTY	4 cut(s)	164, 895, 1026, 1312
AcuI	CTGAAG	2 cut(s)	1359, 1956
AfaI	GTAC	5 cut(s)	15, 135, 1667, 2055, 2750
AfiI	CCNNNNNNNGG	5 cut(s)	29, 95, 572, 1875, 2497
AflIII	ACRYGT	2 cut(s)	2395, 2699
AjiI	CACGTC	1 cut(s)	688
AjnI	CCWGG	2 cut(s)	28, 1075
AjuI	GAANNNNNNNTTGG	2 cut(s)	2031, 2063
AleI	CACNNNNGTG	1 cut(s)	176
AloI	GAACNNNNNNTCC	2 cut(s)	398, 430
Alw21I	GWGCWC	3 cut(s)	1308, 2165, 2377
Alw26I	GTCTC	3 cut(s)	74, 1806, 2931
AlwNI	CAGNNNCTG	2 cut(s)	1171, 3026
AoxI	GGCC	8 cut(s)	1578, 1788, 1863, 2062, 2350, 2728, 2939, 3088
ApoI	RAATTY	4 cut(s)	164, 895, 1026, 1312
ArsI	GACNNNNNNTTYG	2 cut(s)	748, 780
Asp700I	GAANNNNTTC	1 cut(s)	1777
AspLEI	GCGC	1 cut(s)	720
AspS9I	GGNCC	7 cut(s)	406, 1196, 1789, 2072, 2682, 2729, 3088
AsuC2I	CCSGG	1 cut(s)	2216
AsuHPI	GGTGA	4 cut(s)	1053, 1609, 2138, 3089
AsuNHI	GCTAGC	1 cut(s)	1055
AvaII	GGWCC	4 cut(s)	406, 1196, 2072, 2682
BaeGI	GKGCMC	1 cut(s)	2604
BaeI	ACNNNNGTAYC	4 cut(s)	921, 954, 2442, 2475
BamHI	GGATCC	2 cut(s)	2188, 2613
BanII	GRGCYC	1 cut(s)	102
BbsI	GAAGAC	5 cut(s)	344, 682, 1175, 1557, 2142
Bbv12I	GWGCWC	3 cut(s)	1308, 2165, 2377
BbvCI	CCTCAGC	1 cut(s)	1724
BceAI	ACGGC	4 cut(s)	112, 161, 322, 2471
BcgI	CGANNNNNNTGC	4 cut(s)	2241, 2275, 2651, 2685
BciT130I	CCWGG	2 cut(s)	30, 1077
BciVI	GTATCC	4 cut(s)	16, 513, 922, 1697
BclI	TGATCA	2 cut(s)	1603, 2293
BcnI	CCSGG	1 cut(s)	2216
BcoDI	GTCTC	3 cut(s)	74, 1806, 2931
BfmI	CTRYAG	4 cut(s)	1035, 1163, 2016, 3045
BfuAI	ACCTGC	2 cut(s)	1864, 2132
BfuI	GTATCC	4 cut(s)	16, 513, 922, 1697
BglI	GCCNNNNNGGC	1 cut(s)	2727
BglII	AGATCT	2 cut(s)	523, 2181
BmcAI	AGTACT	2 cut(s)	2055, 2750
Bme1390I	CCNGG	3 cut(s)	30, 1077, 2216
Bme18I	GGWCC	4 cut(s)	406, 1196, 2072, 2682
BmgBI	CACGTC	1 cut(s)	688
BmgT120I	GGNCC	7 cut(s)	406, 1196, 1789, 2072, 2682, 2729, 3088
BmiI	GGNNCC	4 cut(s)	101, 1197, 2190, 2615
BmrFI	CCNGG	3 cut(s)	30, 1077, 2216
BmrI	ACTGGG	3 cut(s)	866, 1202, 1777
BmtI	GCTAGC	1 cut(s)	1059
BmuI	ACTGGG	3 cut(s)	866, 1202, 1777
BoxI	GACNNNNGTC	1 cut(s)	63
BpiI	GAAGAC	5 cut(s)	344, 682, 1175, 1557, 2142
Bpu10I	CCTNAGC	2 cut(s)	456, 1724
BpuEI	CTTGAG	3 cut(s)	431, 2928, 3074
BpuMI	CCSGG	1 cut(s)	2216
BsaBI	GATNNNNATC	3 cut(s)	200, 1415, 1813
BsaJI	CCNNGG	2 cut(s)	584, 1860
BsaWI	WCCGGW	2 cut(s)	1868, 2616
BsaXI	ACNNNNNCTCC	2 cut(s)	1090, 1120
Bsc4I	CCNNNNNNNGG	5 cut(s)	29, 95, 572, 1875, 2497
Bse118I	RCCGGY	1 cut(s)	2573
Bse1I	ACTGG	5 cut(s)	861, 1197, 1772, 2074, 2656
Bse3DI	GCAATG	2 cut(s)	390, 2547
Bse8I	GATNNNNATC	3 cut(s)	200, 1415, 1813
BseBI	CCWGG	2 cut(s)	30, 1077
BseDI	CCNNGG	2 cut(s)	584, 1860
BseJI	GATNNNNATC	3 cut(s)	200, 1415, 1813
BseLI	CCNNNNNNNGG	5 cut(s)	29, 95, 572, 1875, 2497
BseMI	GCAATG	2 cut(s)	390, 2547
BseMII	CTCAG	6 cut(s)	447, 746, 900, 1677, 1715, 2342
BseNI	ACTGG	5 cut(s)	861, 1197, 1772, 2074, 2656
BseRI	GAGGAG	1 cut(s)	1894
BseSI	GKGCMC	1 cut(s)	2604
BseYI	CCCAGC	2 cut(s)	1884, 2987
BsgI	GTGCAG	1 cut(s)	2155
BshFI	GGCC	8 cut(s)	1580, 1790, 1865, 2064, 2352, 2730, 2941, 3090
BsiHKAI	GWGCWC	3 cut(s)	1308, 2165, 2377
BsiSI	CCGG	4 cut(s)	1869, 2215, 2574, 2617
BslFI	GGGAC	5 cut(s)	879, 1209, 2005, 2576, 2651
BslI	CCNNNNNNNGG	5 cut(s)	29, 95, 572, 1875, 2497
BsmAI	GTCTC	3 cut(s)	74, 1806, 2931
BsmFI	GGGAC	5 cut(s)	879, 1209, 2005, 2576, 2651
BsmI	GAATGC	1 cut(s)	704
BsnI	GGCC	8 cut(s)	1580, 1790, 1865, 2064, 2352, 2730, 2941, 3090
Bsp1286I	GDGCHC	5 cut(s)	102, 1308, 2165, 2377, 2604
BspACI	CCGC	5 cut(s)	926, 1117, 1836, 2885, 2958
BspANI	GGCC	8 cut(s)	1580, 1790, 1865, 2064, 2352, 2730, 2941, 3090
BspCNI	CTCAG	6 cut(s)	448, 745, 901, 1678, 1716, 2341
BspHI	TCATGA	2 cut(s)	598, 2413
BspLI	GGNNCC	4 cut(s)	101, 1197, 2190, 2615
BspMAI	CTGCAG	3 cut(s)	1039, 1167, 2020
BspMI	ACCTGC	2 cut(s)	1864, 2132
BspOI	GCTAGC	1 cut(s)	1059
BspQI	GCTCTTC	1 cut(s)	1975
BsrBI	CCGCTC	1 cut(s)	1117
BsrDI	GCAATG	2 cut(s)	390, 2547
BsrFI	RCCGGY	1 cut(s)	2573
BsrI	ACTGG	5 cut(s)	861, 1197, 1772, 2074, 2656
BssAI	RCCGGY	1 cut(s)	2573
BssECI	CCNNGG	2 cut(s)	584, 1860
BssT1I	CCWWGG	1 cut(s)	584
Bst2UI	CCWGG	2 cut(s)	30, 1077
Bst6I	CTCTTC	4 cut(s)	996, 1222, 1975, 2334
BstAPI	GCANNNNNTGC	2 cut(s)	2169, 2723
BstC8I	GCNNGC	7 cut(s)	1057, 1119, 2377, 2381, 2719, 2728, 3092
BstDEI	CTNAG	9 cut(s)	456, 467, 611, 732, 909, 1686, 1724, 2057, 2328
BstEII	GGTNACC	1 cut(s)	2126
BstHHI	GCGC	1 cut(s)	720
BstMAI	GTCTC	3 cut(s)	74, 1806, 2931
BstNI	CCWGG	2 cut(s)	30, 1077
BstNSI	RCATGY	5 cut(s)	1745, 1900, 2383, 2703, 2721
BstPAI	GACNNNNGTC	1 cut(s)	63
BstPI	GGTNACC	1 cut(s)	2126
BstSCI	CCNGG	3 cut(s)	28, 1075, 2214
BstSFI	CTRYAG	4 cut(s)	1035, 1163, 2016, 3045
BstSLI	GKGCMC	1 cut(s)	2604
BstV2I	GAAGAC	5 cut(s)	344, 682, 1175, 1557, 2142
BstX2I	RGATCY	5 cut(s)	523, 2116, 2181, 2188, 2613
BstXI	CCANNNNNNTGG	2 cut(s)	273, 514
BstYI	RGATCY	5 cut(s)	523, 2116, 2181, 2188, 2613
BsuI	GTATCC	4 cut(s)	16, 513, 922, 1697
BsuRI	GGCC	8 cut(s)	1580, 1790, 1865, 2064, 2352, 2730, 2941, 3090
BtgZI	GCGATG	1 cut(s)	1336
BtrI	CACGTC	1 cut(s)	688
BtsI	GCAGTG	4 cut(s)	1285, 2079, 2856, 3079
BveI	ACCTGC	2 cut(s)	1864, 2132
Cac8I	GCNNGC	7 cut(s)	1057, 1119, 2377, 2381, 2719, 2728, 3092
CaiI	CAGNNNCTG	2 cut(s)	1171, 3026
CciI	TCATGA	2 cut(s)	598, 2413
CfoI	GCGC	1 cut(s)	720
Cfr10I	RCCGGY	1 cut(s)	2573
Cfr13I	GGNCC	7 cut(s)	406, 1196, 1789, 2072, 2682, 2729, 3088
CseI	GACGC	4 cut(s)	467, 1169, 1478, 2147
Csp6I	GTAC	5 cut(s)	14, 134, 1666, 2054, 2749
CviQI	GTAC	5 cut(s)	14, 134, 1666, 2054, 2749
DdeI	CTNAG	9 cut(s)	456, 467, 611, 732, 909, 1686, 1724, 2057, 2328
EaeI	YGGCCR	1 cut(s)	1578
Eam1104I	CTCTTC	4 cut(s)	996, 1222, 1975, 2334
EarI	CTCTTC	4 cut(s)	996, 1222, 1975, 2334
Eco130I	CCWWGG	1 cut(s)	584
Eco147I	AGGCCT	1 cut(s)	1865
Eco24I	GRGCYC	1 cut(s)	102
Eco47I	GGWCC	4 cut(s)	406, 1196, 2072, 2682
Eco57I	CTGAAG	2 cut(s)	1359, 1956
Eco91I	GGTNACC	1 cut(s)	2126
EcoO109I	RGGNCCY	1 cut(s)	1196
EcoO65I	GGTNACC	1 cut(s)	2126
EcoRII	CCWGG	2 cut(s)	28, 1075
EcoT14I	CCWWGG	1 cut(s)	584
EcoT22I	ATGCAT	2 cut(s)	1663, 2498
EcoT38I	GRGCYC	1 cut(s)	102
ErhI	CCWWGG	1 cut(s)	584
FalI	AAGNNNNNCTT	2 cut(s)	2937, 2969
FaqI	GGGAC	5 cut(s)	879, 1209, 2005, 2576, 2651
FauI	CCCGC	1 cut(s)	1110
FbaI	TGATCA	2 cut(s)	1603, 2293
FblI	GTMKAC	2 cut(s)	285, 315
FriOI	GRGCYC	1 cut(s)	102
GlaI	GCGC	1 cut(s)	719
GsaI	CCCAGC	2 cut(s)	1888, 2991
HaeIII	GGCC	8 cut(s)	1580, 1790, 1865, 2064, 2352, 2730, 2941, 3090
HapII	CCGG	4 cut(s)	1869, 2215, 2574, 2617
HgaI	GACGC	4 cut(s)	467, 1169, 1478, 2147
HhaI	GCGC	1 cut(s)	720
Hin6I	GCGC	1 cut(s)	718
HinP1I	GCGC	1 cut(s)	718
HincII	GTYRAC	4 cut(s)	151, 729, 756, 1335
HindII	GTYRAC	4 cut(s)	151, 729, 756, 1335
HindIII	AAGCTT	1 cut(s)	2591
HinfI	GANTC	8 cut(s)	82, 770, 1253, 1773, 1846, 2510, 2520, 2901
HpaII	CCGG	4 cut(s)	1869, 2215, 2574, 2617
HphI	GGTGA	4 cut(s)	1053, 1609, 2138, 3089
Hpy166II	GTNNAC	9 cut(s)	151, 286, 316, 406, 729, 756, 1335, 2003, 2854
Hpy8I	GTNNAC	9 cut(s)	151, 286, 316, 406, 729, 756, 1335, 2003, 2854
HpyCH4IV	ACGT	5 cut(s)	687, 1394, 1525, 1822, 2395
HpyF3I	CTNAG	9 cut(s)	456, 467, 611, 732, 909, 1686, 1724, 2057, 2328
HpySE526I	ACGT	5 cut(s)	687, 1394, 1525, 1822, 2395
HspAI	GCGC	1 cut(s)	718
Ksp22I	TGATCA	2 cut(s)	1603, 2293
LguI	GCTCTTC	1 cut(s)	1975
LmnI	GCTCC	6 cut(s)	105, 1114, 1303, 1881, 2160, 2906
MaeII	ACGT	5 cut(s)	687, 1394, 1525, 1822, 2395
MbiI	CCGCTC	1 cut(s)	1117
MfeI	CAATTG	2 cut(s)	61, 921
MflI	RGATCY	5 cut(s)	523, 2116, 2181, 2188, 2613
MhlI	GDGCHC	5 cut(s)	102, 1308, 2165, 2377, 2604
MlyI	GAGTC	3 cut(s)	76, 1855, 2504
Mph1103I	ATGCAT	2 cut(s)	1663, 2498
MroXI	GAANNNNTTC	1 cut(s)	1777
MslI	CAYNNNNRTG	4 cut(s)	176, 271, 360, 1355
MspA1I	CMGCKG	3 cut(s)	2021, 2912, 3023
MspI	CCGG	4 cut(s)	1869, 2215, 2574, 2617
MspR9I	CCNGG	3 cut(s)	30, 1077, 2216
MunI	CAATTG	2 cut(s)	61, 921
Mva1269I	GAATGC	1 cut(s)	704
MvaI	CCWGG	2 cut(s)	30, 1077
NciI	CCSGG	1 cut(s)	2216
NheI	GCTAGC	1 cut(s)	1055
NlaIV	GGNNCC	4 cut(s)	101, 1197, 2190, 2615
NmeAIII	GCCGAG	1 cut(s)	1885
NmuCI	GTSAC	3 cut(s)	1597, 2126, 2657
NsiI	ATGCAT	2 cut(s)	1663, 2498
NspI	RCATGY	5 cut(s)	1745, 1900, 2383, 2703, 2721
OliI	CACNNNNGTG	1 cut(s)	176
PacI	TTAATTAA	1 cut(s)	162
PaeI	GCATGC	2 cut(s)	2383, 2721
PagI	TCATGA	2 cut(s)	598, 2413
PceI	AGGCCT	1 cut(s)	1865
PciI	ACATGT	1 cut(s)	2699
PciSI	GCTCTTC	1 cut(s)	1975
PctI	GAATGC	1 cut(s)	704
PdmI	GAANNNNTTC	1 cut(s)	1777
PfeI	GAWTC	5 cut(s)	770, 1253, 1773, 2520, 2901
PfoI	TCCNGGA	1 cut(s)	28
PleI	GAGTC	3 cut(s)	76, 1854, 2504
PpsI	GAGTC	3 cut(s)	76, 1854, 2504
PpuMI	RGGWCCY	1 cut(s)	1196
PscI	ACATGT	1 cut(s)	2699
PshAI	GACNNNNGTC	1 cut(s)	63
PsiI	TTATAA	2 cut(s)	917, 1718
Psp5II	RGGWCCY	1 cut(s)	1196
Psp6I	CCWGG	2 cut(s)	28, 1075
PspEI	GGTNACC	1 cut(s)	2126
PspFI	CCCAGC	2 cut(s)	1884, 2987
PspGI	CCWGG	2 cut(s)	28, 1075
PspN4I	GGNNCC	4 cut(s)	101, 1197, 2190, 2615
PspPI	GGNCC	7 cut(s)	406, 1196, 1789, 2072, 2682, 2729, 3088
PspPPI	RGGWCCY	1 cut(s)	1196
PstI	CTGCAG	3 cut(s)	1039, 1167, 2020
PstNI	CAGNNNCTG	2 cut(s)	1171, 3026
PsuI	RGATCY	5 cut(s)	523, 2116, 2181, 2188, 2613
PvuII	CAGCTG	3 cut(s)	2021, 2912, 3023
RsaI	GTAC	5 cut(s)	15, 135, 1667, 2055, 2750
RsaNI	GTAC	5 cut(s)	14, 134, 1666, 2054, 2749
RseI	CAYNNNNRTG	4 cut(s)	176, 271, 360, 1355
SapI	GCTCTTC	1 cut(s)	1975
Sau96I	GGNCC	7 cut(s)	406, 1196, 1789, 2072, 2682, 2729, 3088
ScaI	AGTACT	2 cut(s)	2055, 2750
SchI	GAGTC	3 cut(s)	76, 1855, 2504
ScrFI	CCNGG	3 cut(s)	30, 1077, 2216
SduI	GDGCHC	5 cut(s)	102, 1308, 2165, 2377, 2604
SfcI	CTRYAG	4 cut(s)	1035, 1163, 2016, 3045
SinI	GGWCC	4 cut(s)	406, 1196, 2072, 2682
SmiMI	CAYNNNNRTG	4 cut(s)	176, 271, 360, 1355
SmlI	CTYRAG	3 cut(s)	410, 2943, 3053
SmoI	CTYRAG	3 cut(s)	410, 2943, 3053
SphI	GCATGC	2 cut(s)	2383, 2721
SseBI	AGGCCT	1 cut(s)	1865
SsiI	CCGC	5 cut(s)	926, 1117, 1836, 2885, 2958
SspI	AATATT	2 cut(s)	218, 2736
StuI	AGGCCT	1 cut(s)	1865
StyD4I	CCNGG	3 cut(s)	28, 1075, 2214
StyI	CCWWGG	1 cut(s)	584
TaiI	ACGT	5 cut(s)	690, 1397, 1528, 1825, 2398
TaqI	TCGA	3 cut(s)	40, 2050, 2266
TaqII	GACCGA	1 cut(s)	714
TatI	WGTACW	2 cut(s)	2053, 2748
TauI	GCSGC	2 cut(s)	1838, 2961
TfiI	GAWTC	5 cut(s)	770, 1253, 1773, 2520, 2901
TseFI	GTSAC	3 cut(s)	1597, 2126, 2657
Tsp45I	GTSAC	3 cut(s)	1597, 2126, 2657
TspGWI	ACGGA	4 cut(s)	282, 2237, 2731, 2801
VpaK11BI	GGWCC	4 cut(s)	406, 1196, 2072, 2682
XapI	RAATTY	4 cut(s)	164, 895, 1026, 1312
XbaI	TCTAGA	2 cut(s)	142, 2335
XceI	RCATGY	5 cut(s)	1745, 1900, 2383, 2703, 2721
XmiI	GTMKAC	2 cut(s)	285, 315
XmnI	GAANNNNTTC	1 cut(s)	1777
ZrmI	AGTACT	2 cut(s)	2055, 2750
Zsp2I	ATGCAT	2 cut(s)	1663, 2498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.