Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007797

Physical Location & Seq

Genomic Coordinates Reverse (-)

30513543 .. 30524958

Gene Structure 11416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1224200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3627 bp

ATGGCTCGGATCTTCGAGCACTTCGTGGTGTGCGGGATCGGACCCGAGATTCGGACCCTCGACGGCGCCAAAGGATTCCACGGCTTCGACACTTATTACCTGCCGTCGCTTCTGGACCAGTACCCTCCGCCCAACCACTCTCTCTACCCTCCTCCTCCGCCTCAGCTCCCCACCTGTGTGTTGCCAGCTGGTGTTTTGTTCTATTCGTCGGGCTTTGATTCCAATGACGCCTACACGTTTCCACGGAGCTACCCCATTGTTCTCACTGAGGGTGATGGGACAAAAATTTATGTCAGTTGCATTGCTTTCCGGGATCCAGTCAGTGAGGATGTTGCTGAAGCTTACTGTATACCTGCAAATTCATTTGCTGATAAATGTATCTGTCTTGTTTCACGGTCACCCAGCTTTCGTTTACTTAAGAATGCACTGGAGGAATTATTTACACTTTGTTTTTCCCCTAATGGAAGTAGCAAACCCTTATGGGAAGTTATTGCATCTTTGGTGTCCACTGTACCTTTGCCTACTCCAGGAAAGGACCGAGTTCTGTTTGCTATTGACAATTGTCTACTTTCTGTGGAGGCTCCACCAAAGGATGGGCTCCCTCATGTTGATATATCGTTTCAGCCATTGGTACAGTGCCTAGATGTTGACAACTTGATTAAATTTTTCACCGCTGTGTTGCTTGAAAGGAGAATACTGATCCGATCAAACAAATATTCACTTTTGACCCTTGTATCAGAAGCCATTTGCCATTTACTTTATCCATTGCGGTGGCAGCAAGTCTACATTCCGTTACTCTTTTTCAGTGGAGTAGACTATATTGATGCGCCTACGCCATATATGATGGGTCTCCATTCAGGCGTTGATACATCTACTTTGGCCATGGATGGTGTAGTTGTTGTTGACCTTGAGTGTAATCGTATCACAACAACGGAAGATATACCTCCAATCCCAGAGCAAGAATTGGGCAATCTGCGTGGAGAGATATCAAAGCTATTGTATCCCAATGTTGTGGGGATAGATCAGATCAAGGATGGTTTATATAGTTCATCTGAGCAATATTCTAAAGCTGGCAATAAACCTTGGGGAGAGGATCACGACCTTCAACTTAGGTTAGTGTTCTTAAAGTTCTTTGCATCACTTTTAAGTGGCTACCGCAACTTCATAGAAATTTCTGCTACTCATGTCTTCAACACTCAAGCATTCTTGACAATGCGCTCTCGGTCAACTGGCCAACCACCAGAACCCATGTTAACGCAGTTTCTGGATTCGCATGGTTTCATGGATTATCTAGAGAGATGTTTGGGTTCTGAAGAAAACACTAATAATCTACTTGACAAGTTACAAGATGCACTTAGAAGGGGCCAAAATCCTATTTCAATTCTTCCCTCAAATTCAGTAGAGCCTGAGATTATAACAATTGCAGATACGGATGTGAGAAATTCAGGAGCAGGTACCAAATACACATATGATAGGTTTCCCTCGGACCTTAGGACTGAAGAGCAAGAAGAAAAGAGGAAGCAGATCCTGGCTGCAGCAAGTGGATTATCCTCTAGCTCACAGTCTGTTCCAGGGGGTAAAGACTCTAAATCAGAAACCCTAACTCCACTGGAGAGGGCTGCGGAAAGGGAGTGCATGGTTTTGGACATAAAAGTTAAGCTGCAGGGTCTGTGGCTTCGTCTTCTGAAATTGGGACCTACTGATGATCCTCTTTCTTCATTTGAATATGGCACAATCCTCGCTTTAATTGAATCTGATGCTGAGGGAATTGGTGGCAGTGGGTTTGTTGAATGTATAAGGGAACACATAAATCAGGGATGGCACTGTCAGTTGACTGAAGGACAATTTCTTGCTGTTAAGGAACTGCTCAAAACAGCTATTAGTCGTGCAACTTCCCGAAATGATATGTTGACAATCAGGGATGCCCTTGAAGTATCCGCTGACATGTATAAGAAGGATGCACATAATGTTTCAGACTACATTGAGCGTCATCTTATATCTTTGTCCATCTGGGAGGAATTACGCTTTTGGGAAGGATACTTTGACTATCTAATGGAACGGTCTTCAAACAAGTCGGCTAACTATGCCTCTTTGGTGGCTGCGCACTTAATTTATGTGGAATCGCACATGGCTGGGTTGGGAATTCCTGATACTGATGCTTGGTACATGATTGAAACGATAGCAGAGAAGAACAATATTGGATACACACAATTTATAAAGCTGAGAGGGTTCTTGTCGCATATCGAGCACCTACTCATTGGTTATTGGGGTATCACTAATTTCAAGGGTCAATCCATGTTGTCTCATGGATTAGCATCTCCACGTTCAAAAGATGCTACAGTTGAGAATCAGCAGCCTGCTGAAGCTTCTGGTGTTGGAAGGAGCTGGGTTCAGAGCATGTTCAGCAGGGATACTGCATCCAGAACCACCTCTTTAAGTCGTGTTCGTAAATGGACTTCTGATGGTGGCTCTTCAGCTGCAAATGAAAATGGGACTCCTCGTAAGCAAGATTTGTCAGCCACTGGCCAGAAGAAAACTCAAACCAATGTTCGCTTACTTAGAGGTCATAGTGGTTCCGTCACTGCTTTACATTGTGTAACGAAGAGAGAGGTTTGGGATCTTGTGGGTGACCGGGAAGATGCCGGTTTCTTTATCAGTGGAAGCACAGACTCCACTGTTAAGATCTGGGATCCTAGTTTTCGTGGTAATGAACTTCGAGCAACCTTGAAAGGACATACAAGAGCTGTCCGTGCAATAAGTTCTGATCGAGGAAAAGTAGTATCAGGTTCGGATGATCAAACTGTTTTAGTATGGGATAAGCAAACTACTCAGCTTCTAGAAGAGTTGAAAGGCCATGATGCACCAGTTAGCTGTGCACGCATGCTGTCTGGTGAACGTGTACTTACTGCTGCTCATGATGGAACTGTAAAGATGTGGGATGTCAGAACTGACACTTGCGTGGCAACCGTTGGTCGCTGCTCTAGTGCTGTTCTATGTATGGAATATGATGATTCCACAGGAATTTTAGCAGCTGGTGGTAGAGATGTCGTCGCAAACATCTGGGACATTCGTGCTGGCAAGCCAATGCACAAGCTTGTAGGGCATACCAAATGGATTCGGTCGATTAGAATGGTGGGGGACACAGTTGTTACTGGTAGTGATGACTGGACTGCAAGGATGTGGTCTGTTTCCCGAGGAACTTGTGATGCTGTTCTAGCATGCCATGCTGGCCCCATATTAAGCGTTGAGTATTCCACACTTGACAAAGGAATAATTACAGGTTCAACTGATGGGCTACTCCGCTTTTGGGAAAATGTCGAGGGAGGTATAAGATGCGTAAAAAATGTTACTGTCCACACCGCTGCTGTATTGTCCATCAATGCTGCAGAACACTGGTTAGGAATTGGAGCAGCTGATAATTCCATGTCTCTCTTTCATCGGCCTCAAGAGAGACTCGGTAGCTCCTCTAGCGCGGGATCAAAAATGGCCGGGTGGCAACTATATAGAACACCACAAAGAACAGTTGCTGTGGTAAGATGTATTGCAACGGACCTCGAGAGGAAAAGGATATGCAGTGGCGGTCGTAATGGACTTCTTAGGCTTTGGGAAGCCACCATAAACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0002682 GO:0002683 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0007049 GO:0007275 GO:0008150 GO:0009791 GO:0009825 GO:0009888 GO:0009987 GO:0010235 GO:0010374 GO:0010440 GO:0016020 GO:0016049 GO:0022402 GO:0030135 GO:0030136 GO:0031347 GO:0031348 GO:0031410 GO:0031461 GO:0031982 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045824 GO:0048519 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0051301 GO:0061640 GO:0065007 GO:0071944 GO:0080008 GO:0080134 GO:0090558 GO:0097708 GO:1902410 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DENN WD40 dDENN uDENN

Protein Analysis

1209

Amino Acids

133.55

Weight (kDa)

5.86

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
uDENN	PF03456	35 - 103	1.3e-11	uDENN domain
DENN	PF02141	123 - 304	1.8e-43	DENN (AEX-3) domain
Beta-prop_WDR5	PF25175	880 - 990	3.3e-21	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	880 - 980	5e-09	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	881 - 991	2e-22	WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	881 - 1046	3.6e-07	MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st	PF24817	882 - 990	5.3e-11	WDHD1 first WD40 domain
Beta-prop_TEP1_2nd	PF25047	883 - 1079	1.1e-17	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	884 - 1119	7.7e-33	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	884 - 985	6.2e-06	WDR36/Utp21 second beta-propeller domain
WDR55	PF24796	885 - 1102	2.9e-20	WDR55
Beta-prop_WDR36-Utp21_1st	PF25171	900 - 1103	2e-13	WDR36/Utp21 first beta-propeller
WD40	PF00400	905 - 939	3.3e-06	WD domain, G-beta repeat
Beta-prop_WDR41	PF25178	907 - 1022	4e-09	WDR41
EIF3I	PF24805	908 - 1005	6.1e-07	EIF3I
WD40_MABP1-WDR62_1st	PF24780	916 - 1016	7.4e-06	MABP1/WDR62 first WD40 domain
WD40_Prp19	PF24814	920 - 998	7e-11	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	921 - 1042	4.7e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	923 - 1068	3.4e-15	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	949 - 1145	1e-13	G protein beta WD-40 repeat protein
Beta-prop_DCAF4	PF23761	952 - 1027	6.6e-07	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	965 - 1067	2.5e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	993 - 1152	1.5e-10	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	993 - 1127	1.2e-20	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	995 - 1104	6.7e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	996 - 1117	1.8e-14	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	1046 - 1118	4e-07	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007797)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49040 AT1G49040 AT1G49040 AT1G49040
fragaria_vesca	FvH4_5g12450 FvH4_5g12450
malus_domestica	MD14G1224200.v1.1
prunus_persica	Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1
pyrus_communis	pycom06g19080 pycom14g18580
rosa_chinensis	RchiOBHm_Chr7g0185021
rosa_laevigata	RLG00000004938
rosa_multiflora	Rmu_ssc0000289.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269260
rosa_rugosa	Rorug06G0466200
rosa_samantha	Rh7AG076700 Rh7BG070700 Rh7CG072200 Rh7DG071200
rosa_wichuraiana	Rw7G005890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1415, 2216
Acc16I	TGCGCA	1 cut(s)	2103
Acc36I	ACCTGC	3 cut(s)	108, 361, 1442
Acc65I	GGTACC	1 cut(s)	1454
AccB1I	GGYRCC	2 cut(s)	65, 1454
AccB7I	CCANNNNNTGG	1 cut(s)	593
AccI	GTMKAC	4 cut(s)	349, 565, 783, 813
AccII	CGCG	1 cut(s)	3473
AcoI	YGGCCR	4 cut(s)	879, 1231, 2524, 3486
AcsI	RAATTY	8 cut(s)	285, 358, 662, 1170, 1393, 1441, 2142, 3021
AcuI	CTGAAG	6 cut(s)	357, 1332, 1518, 1857, 2382, 2457
AcyI	GRCGYC	2 cut(s)	66, 228
AdeI	CACNNNGTG	1 cut(s)	25
AfaI	GTAC	6 cut(s)	122, 513, 633, 1456, 2165, 2901
AfiI	CCNNNNNNNGG	4 cut(s)	51, 527, 593, 3307
AflII	CTTAAG	1 cut(s)	416
AflIII	ACRYGT	3 cut(s)	234, 1944, 2896
AjnI	CCWGG	3 cut(s)	526, 1527, 1570
AleI	CACNNNNGTG	3 cut(s)	176, 674, 2957
Alw21I	GWGCWC	3 cut(s)	21, 2250, 2878
Alw26I	GTCTC	4 cut(s)	854, 2307, 3432, 3445
Alw44I	GTGCAC	1 cut(s)	2874
AlwNI	CAGNNNCTG	5 cut(s)	1264, 1528, 1669, 2522, 3527
Ama87I	CYCGRG	3 cut(s)	44, 3192, 3554
AoxI	GGCC	8 cut(s)	879, 1231, 1363, 2524, 2851, 3229, 3440, 3486
ApaLI	GTGCAC	1 cut(s)	2874
ApoI	RAATTY	8 cut(s)	285, 358, 662, 1170, 1393, 1441, 2142, 3021
ArsI	GACNNNNNNTTYG	2 cut(s)	33, 65
Asp718I	GGTACC	1 cut(s)	1454
AspLEI	GCGC	5 cut(s)	68, 829, 1218, 2104, 3473
AspS9I	GGNCC	9 cut(s)	41, 54, 115, 535, 1363, 1486, 1694, 3230, 3550
AsuC2I	CCSGG	3 cut(s)	311, 2633, 3490
AsuHPI	GGTGA	5 cut(s)	284, 390, 661, 2639, 2903
AvaI	CYCGRG	3 cut(s)	44, 3192, 3554
AvaII	GGWCC	7 cut(s)	41, 54, 115, 535, 1486, 1694, 3550
AxyI	CCTNAGG	1 cut(s)	1490
BaeGI	GKGCMC	1 cut(s)	2878
BaeI	ACNNNNGTAYC	4 cut(s)	717, 750, 1419, 1452
BalI	TGGCCA	3 cut(s)	881, 1233, 2526
BamHI	GGATCC	2 cut(s)	313, 2689
BanI	GGYRCC	2 cut(s)	65, 1454
BanII	GRGCYC	1 cut(s)	600
BarI	GAAGNNNNNNTAC	2 cut(s)	2440, 2472
BbsI	GAAGAC	3 cut(s)	1180, 1673, 2055
Bbv12I	GWGCWC	3 cut(s)	21, 2250, 2878
BbvCI	CCTCAGC	2 cut(s)	162, 1761
BceAI	ACGGC	3 cut(s)	79, 88, 97
BcgI	CGANNNNNNTGC	2 cut(s)	1720, 1754
BciT130I	CCWGG	3 cut(s)	528, 1529, 1572
BciVI	GTATCC	5 cut(s)	1011, 1945, 2030, 2195, 2404
BclI	TGATCA	1 cut(s)	2794
BcnI	CCSGG	3 cut(s)	311, 2633, 3490
BcoDI	GTCTC	4 cut(s)	854, 2307, 3432, 3445
BfaI	CTAG	8 cut(s)	641, 1292, 1554, 2694, 2837, 2982, 3215, 3468
BfmI	CTRYAG	4 cut(s)	1533, 1661, 2337, 3384
BfoI	RGCGCY	1 cut(s)	69
BfrI	CTTAAG	1 cut(s)	416
BfuAI	ACCTGC	3 cut(s)	108, 361, 1442
BfuI	GTATCC	5 cut(s)	1011, 1945, 2030, 2195, 2404
BglI	GCCNNNNNGGC	1 cut(s)	3228
BglII	AGATCT	1 cut(s)	2682
Bme1390I	CCNGG	6 cut(s)	311, 528, 1529, 1572, 2633, 3490
Bme18I	GGWCC	7 cut(s)	41, 54, 115, 535, 1486, 1694, 3550
BmeT110I	CYCGRG	3 cut(s)	44, 3192, 3554
BmgT120I	GGNCC	9 cut(s)	41, 54, 115, 535, 1363, 1486, 1694, 3230, 3550
BmrFI	CCNGG	6 cut(s)	311, 528, 1529, 1572, 2633, 3490
BoxI	GACNNNNGTC	1 cut(s)	561
BpiI	GAAGAC	3 cut(s)	1180, 1673, 2055
BpmI	CTGGAG	3 cut(s)	449, 510, 1631
Bpu10I	CCTNAGC	2 cut(s)	162, 1761
BpuEI	CTTGAG	3 cut(s)	929, 1182, 3429
BpuMI	CCSGG	3 cut(s)	311, 2633, 3490
BsaBI	GATNNNNATC	1 cut(s)	2314
BsaHI	GRCGYC	2 cut(s)	66, 228
BsaI	GGTCTC	1 cut(s)	854
BsaJI	CCNNGG	7 cut(s)	79, 242, 882, 1082, 1482, 1571, 3193
BsaXI	ACNNNNNCTCC	2 cut(s)	1588, 1618
Bsc4I	CCNNNNNNNGG	4 cut(s)	51, 527, 593, 3307
Bse118I	RCCGGY	1 cut(s)	2642
Bse21I	CCTNAGG	1 cut(s)	1490
Bse3DI	GCAATG	2 cut(s)	300, 764
Bse8I	GATNNNNATC	1 cut(s)	2314
BseBI	CCWGG	3 cut(s)	528, 1529, 1572
BseDI	CCNNGG	7 cut(s)	79, 242, 882, 1082, 1482, 1571, 3193
BseJI	GATNNNNATC	1 cut(s)	2314
BseLI	CCNNNNNNNGG	4 cut(s)	51, 527, 593, 3307
BseMI	GCAATG	2 cut(s)	300, 764
BseMII	CTCAG	7 cut(s)	176, 258, 1044, 1398, 1752, 2213, 2843
BseRI	GAGGAG	4 cut(s)	141, 144, 2487, 3454
BseSI	GKGCMC	1 cut(s)	2878
BseYI	CCCAGC	3 cut(s)	401, 2132, 2385
Bsh1236I	CGCG	1 cut(s)	3473
Bsh1285I	CGRYCG	2 cut(s)	3122, 3583
BshFI	GGCC	8 cut(s)	881, 1233, 1365, 2526, 2853, 3231, 3442, 3488
BshNI	GGYRCC	2 cut(s)	65, 1454
BsiEI	CGRYCG	2 cut(s)	3122, 3583
BsiHKAI	GWGCWC	3 cut(s)	21, 2250, 2878
BsiHKCI	CYCGRG	3 cut(s)	44, 3192, 3554
BsiSI	CCGG	4 cut(s)	310, 2632, 2643, 3489
BslFI	GGGAC	5 cut(s)	292, 1707, 2506, 3077, 3152
BslI	CCNNNNNNNGG	4 cut(s)	51, 527, 593, 3307
BsmAI	GTCTC	4 cut(s)	854, 2307, 3432, 3445
BsmFI	GGGAC	5 cut(s)	292, 1707, 2506, 3077, 3152
BsmI	GAATGC	2 cut(s)	427, 1202
BsnI	GGCC	8 cut(s)	881, 1233, 1365, 2526, 2853, 3231, 3442, 3488
Bso31I	GGTCTC	1 cut(s)	854
BsoBI	CYCGRG	3 cut(s)	44, 3192, 3554
Bsp1286I	GDGCHC	4 cut(s)	21, 600, 2250, 2878
Bsp19I	CCATGG	1 cut(s)	882
BspANI	GGCC	8 cut(s)	881, 1233, 1365, 2526, 2853, 3231, 3442, 3488
BspCNI	CTCAG	7 cut(s)	175, 259, 1045, 1399, 1753, 2214, 2842
BspFNI	CGCG	1 cut(s)	3473
BspHI	TCATGA	1 cut(s)	2914
BspMAI	CTGCAG	3 cut(s)	1537, 1665, 3388
BspMI	ACCTGC	3 cut(s)	108, 361, 1442
BspQI	GCTCTTC	2 cut(s)	1494, 2476
BspT107I	GGYRCC	2 cut(s)	65, 1454
BspTI	CTTAAG	1 cut(s)	416
BspTNI	GGTCTC	1 cut(s)	854
BsrDI	GCAATG	2 cut(s)	300, 764
BsrFI	RCCGGY	1 cut(s)	2642
BssAI	RCCGGY	1 cut(s)	2642
BssECI	CCNNGG	7 cut(s)	79, 242, 882, 1082, 1482, 1571, 3193
BssNAI	GTATAC	1 cut(s)	350
BssNI	GRCGYC	2 cut(s)	66, 228
BssT1I	CCWWGG	2 cut(s)	882, 1082
Bst1107I	GTATAC	1 cut(s)	350
Bst2UI	CCWGG	3 cut(s)	528, 1529, 1572
Bst6I	CTCTTC	4 cut(s)	1494, 2476, 2597, 2835
BstACI	GRCGYC	2 cut(s)	66, 228
BstAFI	CTTAAG	1 cut(s)	416
BstAPI	GCANNNNNTGC	1 cut(s)	3224
BstC8I	GCNNGC	9 cut(s)	186, 1072, 2358, 2878, 2882, 3076, 3080, 3220, 3229
BstDSI	CCRYGG	3 cut(s)	79, 242, 882
BstEII	GGTNACC	2 cut(s)	396, 2627
BstENI	CCTNNNNNAGG	1 cut(s)	525
BstFNI	CGCG	1 cut(s)	3473
BstH2I	RGCGCY	1 cut(s)	69
BstHHI	GCGC	5 cut(s)	68, 829, 1218, 2104, 3473
BstMAI	GTCTC	4 cut(s)	854, 2307, 3432, 3445
BstMCI	CGRYCG	2 cut(s)	3122, 3583
BstNI	CCWGG	3 cut(s)	528, 1529, 1572
BstNSI	RCATGY	4 cut(s)	1948, 2401, 2884, 3222
BstPAI	GACNNNNGTC	1 cut(s)	561
BstPI	GGTNACC	2 cut(s)	396, 2627
BstSCI	CCNGG	6 cut(s)	309, 526, 1527, 1570, 2631, 3488
BstSFI	CTRYAG	4 cut(s)	1533, 1661, 2337, 3384
BstSLI	GKGCMC	1 cut(s)	2878
BstUI	CGCG	1 cut(s)	3473
BstV2I	GAAGAC	3 cut(s)	1180, 1673, 2055
BstX2I	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
BstXI	CCANNNNNNTGG	2 cut(s)	771, 1012
BstYI	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
BstZ17I	GTATAC	1 cut(s)	350
Bsu36I	CCTNAGG	1 cut(s)	1490
BsuI	GTATCC	5 cut(s)	1011, 1945, 2030, 2195, 2404
BsuRI	GGCC	8 cut(s)	881, 1233, 1365, 2526, 2853, 3231, 3442, 3488
BtgI	CCRYGG	3 cut(s)	79, 242, 882
BtsI	GCAGTG	3 cut(s)	1783, 2580, 3580
BveI	ACCTGC	3 cut(s)	108, 361, 1442
Cac8I	GCNNGC	9 cut(s)	186, 1072, 2358, 2878, 2882, 3076, 3080, 3220, 3229
CaiI	CAGNNNCTG	5 cut(s)	1264, 1528, 1669, 2522, 3527
CciI	TCATGA	1 cut(s)	2914
CfoI	GCGC	5 cut(s)	68, 829, 1218, 2104, 3473
Cfr10I	RCCGGY	1 cut(s)	2642
Cfr13I	GGNCC	9 cut(s)	41, 54, 115, 535, 1363, 1486, 1694, 3230, 3550
CseI	GACGC	2 cut(s)	236, 1976
Csp6I	GTAC	6 cut(s)	121, 512, 632, 1455, 2164, 2900
CspCI	CAANNNNNGTGG	2 cut(s)	958, 993
CviQI	GTAC	6 cut(s)	121, 512, 632, 1455, 2164, 2900
DinI	GGCGCC	1 cut(s)	67
DraIII	CACNNNGTG	1 cut(s)	25
EaeI	YGGCCR	4 cut(s)	879, 1231, 2524, 3486
Eam1104I	CTCTTC	4 cut(s)	1494, 2476, 2597, 2835
EarI	CTCTTC	4 cut(s)	1494, 2476, 2597, 2835
EciI	GGCGGA	2 cut(s)	117, 147
Eco130I	CCWWGG	2 cut(s)	882, 1082
Eco24I	GRGCYC	1 cut(s)	600
Eco31I	GGTCTC	1 cut(s)	854
Eco32I	GATATC	1 cut(s)	987
Eco47I	GGWCC	7 cut(s)	41, 54, 115, 535, 1486, 1694, 3550
Eco57I	CTGAAG	6 cut(s)	357, 1332, 1518, 1857, 2382, 2457
Eco81I	CCTNAGG	1 cut(s)	1490
Eco88I	CYCGRG	3 cut(s)	44, 3192, 3554
Eco91I	GGTNACC	2 cut(s)	396, 2627
EcoNI	CCTNNNNNAGG	1 cut(s)	525
EcoO109I	RGGNCCY	1 cut(s)	1694
EcoO65I	GGTNACC	2 cut(s)	396, 2627
EcoRI	GAATTC	1 cut(s)	2142
EcoRII	CCWGG	3 cut(s)	526, 1527, 1570
EcoRV	GATATC	1 cut(s)	987
EcoT14I	CCWWGG	2 cut(s)	882, 1082
EcoT38I	GRGCYC	1 cut(s)	600
EgeI	GGCGCC	1 cut(s)	67
EheI	GGCGCC	1 cut(s)	67
ErhI	CCWWGG	2 cut(s)	882, 1082
FalI	AAGNNNNNCTT	2 cut(s)	1338, 1370
FaqI	GGGAC	5 cut(s)	292, 1707, 2506, 3077, 3152
FauI	CCCGC	2 cut(s)	26, 3466
FauNDI	CATATG	1 cut(s)	1468
FbaI	TGATCA	1 cut(s)	2794
FblI	GTMKAC	4 cut(s)	349, 565, 783, 813
FriOI	GRGCYC	1 cut(s)	600
FspBI	CTAG	8 cut(s)	641, 1292, 1554, 2694, 2837, 2982, 3215, 3468
FspI	TGCGCA	1 cut(s)	2103
GlaI	GCGC	5 cut(s)	67, 828, 1217, 2103, 3472
GsaI	CCCAGC	3 cut(s)	405, 2136, 2389
GsuI	CTGGAG	3 cut(s)	449, 510, 1631
HaeII	RGCGCY	1 cut(s)	69
HaeIII	GGCC	8 cut(s)	881, 1233, 1365, 2526, 2853, 3231, 3442, 3488
HapII	CCGG	4 cut(s)	310, 2632, 2643, 3489
HgaI	GACGC	2 cut(s)	236, 1976
HhaI	GCGC	5 cut(s)	68, 829, 1218, 2104, 3473
Hin1I	GRCGYC	2 cut(s)	66, 228
Hin6I	GCGC	5 cut(s)	66, 827, 1216, 2102, 3471
HinP1I	GCGC	5 cut(s)	66, 827, 1216, 2102, 3471
HincII	GTYRAC	6 cut(s)	649, 904, 1227, 1254, 1833, 1911
HindII	GTYRAC	6 cut(s)	649, 904, 1227, 1254, 1833, 1911
HindIII	AAGCTT	3 cut(s)	339, 2364, 3092
HpaI	GTTAAC	1 cut(s)	1254
HpaII	CCGG	4 cut(s)	310, 2632, 2643, 3489
HphI	GGTGA	5 cut(s)	284, 390, 661, 2639, 2903
Hpy99I	CGWCG	4 cut(s)	65, 109, 211, 3053
HpyAV	CCTTC	6 cut(s)	1112, 1353, 1832, 1948, 2027, 2373
HpyCH4IV	ACGT	3 cut(s)	236, 2323, 2896
HpySE526I	ACGT	3 cut(s)	236, 2323, 2896
Hsp92I	GRCGYC	2 cut(s)	66, 228
HspAI	GCGC	5 cut(s)	66, 827, 1216, 2102, 3471
KasI	GGCGCC	1 cut(s)	65
KpnI	GGTACC	1 cut(s)	1458
Ksp22I	TGATCA	1 cut(s)	2794
KspAI	GTTAAC	1 cut(s)	1254
LguI	GCTCTTC	2 cut(s)	1494, 2476
LmnI	GCTCC	8 cut(s)	171, 246, 586, 603, 1448, 2382, 3407, 3467
MaeI	CTAG	8 cut(s)	641, 1292, 1554, 2694, 2837, 2982, 3215, 3468
MaeII	ACGT	3 cut(s)	236, 2323, 2896
MaeIII	GTNAC	8 cut(s)	396, 792, 1341, 2578, 2596, 2627, 3148, 3346
MfeI	CAATTG	2 cut(s)	559, 1419
MflI	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
MhlI	GDGCHC	4 cut(s)	21, 600, 2250, 2878
MlsI	TGGCCA	3 cut(s)	881, 1233, 2526
MluNI	TGGCCA	3 cut(s)	881, 1233, 2526
Mly113I	GGCGCC	1 cut(s)	66
MlyI	GAGTC	4 cut(s)	1577, 2488, 2663, 3447
MmeI	TCCRAC	1 cut(s)	2356
Mox20I	TGGCCA	3 cut(s)	881, 1233, 2526
MscI	TGGCCA	3 cut(s)	881, 1233, 2526
MslI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 2957
Msp20I	TGGCCA	3 cut(s)	881, 1233, 2526
MspA1I	CMGCKG	7 cut(s)	188, 674, 1940, 2477, 3032, 3362, 3413
MspCI	CTTAAG	1 cut(s)	416
MspI	CCGG	4 cut(s)	310, 2632, 2643, 3489
MspR9I	CCNGG	6 cut(s)	311, 528, 1529, 1572, 2633, 3490
MunI	CAATTG	2 cut(s)	559, 1419
Mva1269I	GAATGC	2 cut(s)	427, 1202
MvaI	CCWGG	3 cut(s)	528, 1529, 1572
MvnI	CGCG	1 cut(s)	3473
NarI	GGCGCC	1 cut(s)	66
NciI	CCSGG	3 cut(s)	311, 2633, 3490
NcoI	CCATGG	1 cut(s)	882
NdeI	CATATG	1 cut(s)	1468
NmuCI	GTSAC	3 cut(s)	396, 2578, 2627
NsbI	TGCGCA	1 cut(s)	2103
NspI	RCATGY	4 cut(s)	1948, 2401, 2884, 3222
OliI	CACNNNNGTG	3 cut(s)	176, 674, 2957
PaeI	GCATGC	2 cut(s)	2884, 3222
PaeR7I	CTCGAG	1 cut(s)	3554
PagI	TCATGA	1 cut(s)	2914
PciI	ACATGT	1 cut(s)	1944
PciSI	GCTCTTC	2 cut(s)	1494, 2476
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PctI	GAATGC	2 cut(s)	427, 1202
PfeI	GAWTC	9 cut(s)	49, 75, 218, 1268, 1751, 2120, 2347, 3011, 3115
PflMI	CCANNNNNTGG	1 cut(s)	593
PfoI	TCCNGGA	2 cut(s)	309, 526
PleI	GAGTC	4 cut(s)	1577, 2488, 2663, 3447
PluTI	GGCGCC	1 cut(s)	69
PpsI	GAGTC	4 cut(s)	1577, 2488, 2663, 3447
PpuMI	RGGWCCY	1 cut(s)	1694
PscI	ACATGT	1 cut(s)	1944
PshAI	GACNNNNGTC	1 cut(s)	561
PsiI	TTATAA	2 cut(s)	1415, 2216
Psp5II	RGGWCCY	1 cut(s)	1694
Psp6I	CCWGG	3 cut(s)	526, 1527, 1570
PspEI	GGTNACC	2 cut(s)	396, 2627
PspFI	CCCAGC	3 cut(s)	401, 2132, 2385
PspGI	CCWGG	3 cut(s)	526, 1527, 1570
PspPI	GGNCC	9 cut(s)	41, 54, 115, 535, 1363, 1486, 1694, 3230, 3550
PspPPI	RGGWCCY	1 cut(s)	1694
PstI	CTGCAG	3 cut(s)	1537, 1665, 3388
PstNI	CAGNNNCTG	5 cut(s)	1264, 1528, 1669, 2522, 3527
PsuI	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
PvuII	CAGCTG	4 cut(s)	188, 2477, 3032, 3413
RsaI	GTAC	6 cut(s)	122, 513, 633, 1456, 2165, 2901
RsaNI	GTAC	6 cut(s)	121, 512, 632, 1455, 2164, 2900
RseI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 2957
SapI	GCTCTTC	2 cut(s)	1494, 2476
Sau96I	GGNCC	9 cut(s)	41, 54, 115, 535, 1363, 1486, 1694, 3230, 3550
SchI	GAGTC	4 cut(s)	1577, 2488, 2663, 3447
ScrFI	CCNGG	6 cut(s)	311, 528, 1529, 1572, 2633, 3490
SduI	GDGCHC	4 cut(s)	21, 600, 2250, 2878
SfcI	CTRYAG	4 cut(s)	1533, 1661, 2337, 3384
SfoI	GGCGCC	1 cut(s)	67
Sfr274I	CTCGAG	1 cut(s)	3554
SinI	GGWCC	7 cut(s)	41, 54, 115, 535, 1486, 1694, 3550
SlaI	CTCGAG	1 cut(s)	3554
SmiMI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 2957
SmlI	CTYRAG	5 cut(s)	416, 908, 1197, 3444, 3554
SmoI	CTYRAG	5 cut(s)	416, 908, 1197, 3444, 3554
SphI	GCATGC	2 cut(s)	2884, 3222
SspDI	GGCGCC	1 cut(s)	65
SspI	AATATT	3 cut(s)	716, 1061, 2197
SspMI	CTAG	8 cut(s)	641, 1292, 1554, 2694, 2837, 2982, 3215, 3468
StyD4I	CCNGG	6 cut(s)	309, 526, 1527, 1570, 2631, 3488
StyI	CCWWGG	2 cut(s)	882, 1082
TaiI	ACGT	3 cut(s)	239, 2326, 2899
TaqI	TCGA	9 cut(s)	15, 60, 87, 2244, 2716, 2767, 3122, 3318, 3555
TaqII	GACCGA	3 cut(s)	552, 1212, 3108
TatI	WGTACW	1 cut(s)	2899
TfiI	GAWTC	9 cut(s)	49, 75, 218, 1268, 1751, 2120, 2347, 3011, 3115
TseFI	GTSAC	3 cut(s)	396, 2578, 2627
Tsp45I	GTSAC	3 cut(s)	396, 2578, 2627
TspDTI	ATGAA	8 cut(s)	351, 1038, 1153, 1270, 1707, 2499, 2724, 3425
TspGWI	ACGGA	7 cut(s)	259, 780, 947, 1445, 2566, 2738, 3563
Van91I	CCANNNNNTGG	1 cut(s)	593
Vha464I	CTTAAG	1 cut(s)	416
VneI	GTGCAC	1 cut(s)	2874
VpaK11BI	GGWCC	7 cut(s)	41, 54, 115, 535, 1486, 1694, 3550
XagI	CCTNNNNNAGG	1 cut(s)	525
XapI	RAATTY	8 cut(s)	285, 358, 662, 1170, 1393, 1441, 2142, 3021
XbaI	TCTAGA	2 cut(s)	1291, 2836
XceI	RCATGY	4 cut(s)	1948, 2401, 2884, 3222
XhoI	CTCGAG	1 cut(s)	3554
XmiI	GTMKAC	4 cut(s)	349, 565, 783, 813
XspI	CTAG	8 cut(s)	641, 1292, 1554, 2694, 2837, 2982, 3215, 3468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.