Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0007797

Physical Location & Seq

Genomic Coordinates Forward (+)

62172138 .. 62184492

Gene Structure 12355 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00269260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3624 bp

ATGGCTCAGATCTTCGAGCACTTCGTCGTCTGCGGTATCGGACCGGAGATCCGAACCCTCGACGGCGCCAGAGGCTTCCACGGCTTCGCCGAAATGTACCTGCCGTCTTTGCTCGACCAGTTCCCTCCCGCCAACCACACTCTTTACCCTCCTCCGCCGCCTCAGCTCCCCACCTGTGTGTTGCCGGCTGGCGTGTCGTTCTACTCCTCAGGATTTGATTCCAGTGATGCGACGACGTTTCCACGGAGCTATCCCATTGTTCTAACTGAGGGTGATGGGTCAAAAATCTACGTTAGTTGCATTGCTTTCCGGGATCCGGTCAGTGAGGATATTGCTGAAGCTTATTGTATACCGGCAAATTCGTTTGCTGACAAATGTATCTGTCTAGTTTCGCGGTCACCTAGTCTCCGTTTACTTAAAAATGTACTGGAGGAGTTGTTTGCACTGTGTTTTTCCTCTAGTGGAAGCAGCAAACCATTATGGGATATTATTGCGTCTATGGTATCCAATGTACCTCTGCCTACTCCTGGAAAAGATCGAGTTTTGTTTGCCATCGACAATTGTCTGCTTTCTGTGGAGACTCCACCGAAGGATGGGCTCCCTTATGTTGATATATCATTTCAGCCGTTGGTACAGTGTCTAGATGTTGACAACTTAATTAAATTTTTCACAGCCGTGCTGCTTGAAAGGAGGATTTTGATCCGATCACACAAATATTCACTTTTGACCCTTGCATCAGAAGCCATTTGCTACTTAATTTATCCATTTCGGTGGCAGCAAGTCTACATTCCGTTACTCTTTTTCAGTGGAGTAGACTATATTGATGCCCCTACACCGTATATGATGGGTCTTCATTCTGGTGTTGATACATCTACTCTAGCAATGGATGGTGTAGTTATTGTGGACCTTGAGTTCAATCGTATCACAACAACAGAGGATATACCTGCTATTCCCGAGCCAGAACTAAGCTGTTTGCGTCAGGAAATAACGCAGCTATTGTATCCCAATGTTGTGGGGATAGATCTGATGAAGGATGGTCTTTTCAGTTCATCTGAGCAATATCCTAAACTTGGAAACAAACCTTGGGGAGAGGATCATGACCTACAACTTAGGCTAGTGTTCTTAAAGTTCTTTGCATCACTTTTAAGTGGCTATCGGAACTTCATAGAACTTTCTGCTACTCACGTCTTCAATGTTCAAGCATTCTTGAAGATGCGCTCTCGGTCAACTCAGCAACCCCCAGAACCCATGTTGACACAATTTTTGGAATCACATGGTTTCATGGATTATCTGGAAAGAGGTTTGGGTTCTGATGAAAACAGCAATAATCTACTTGACAAGTTACAAGATGCACTGGGAAGGGGCCAAAATCCTATTTCAATTCTTCCTTCAAATTCAGTTGAGCCTGAGATTATAACTATTGCGGATAGAGATGTGGGAACATCAGGGTCAGGTGGCACATACACTTATGACAGGTTTCCCTCAAACATTAGGAAAGAAGAGGAAGAAGAAAAGAGGAAGCAAATTCTTGCTGCAGCAAGTGGTTTATCTGCTAGCTCACCTTCTGTCCTTCCTGGTAAAGACCCCAAATCAGAAACTCTAACTCCATTGGAGCGGGCTGCTGAAAGGGAATGTATGGTTTTGGACATAAAAGTTAAGCTGCAGGGTCTGTGGCTGCGTCTTCTAAAACTGGGACCTACTGATGATCCTCTTTCTTCATTTGAATATGGAACAATCCTTGCTTTAATTGAATCTGATGCAGAAGGAATTGGTGGCAGTGGGTTTGTTGAATGTATAAGGGAGCACATAAATTCGGGATGGCACTGTCAGTTGACTGAAGAACAATTTATCGCTGTGAAGGAACTGCTGAAAACAGCTATCAGTCGTGCAACGTCCAGAAATGATTGGATGACTATCAGAGATGCCCTTGAGGTGTCTGCTGAAATGTTTAAGAAGGATGGAAATAATGTTTCAGATTATGTTCAGCGTCATCTTATATCTTTGTCCATTTGGGAGGAATTACGTTTCTGGGAAAGCTACTTTGACTGTCTAATGGAACGGTCTTCAAACAAGTCGGCCAACTATGCCTCTTTGGTGACTGATCAGCTATCTTTGGTGGAATTACACATGGCTGGACTGGGACTTCCTGATCTTGATGCATGGTACATGATTGAAACAATAGCTGAGAAGAACAACATTGGATACACACAATTTATAAAGCTGAGGGGGTTATTGTCGCATGTTCAACAACTACGAATTGGTTACTGGGGAATCAGTTCTTTCAAGGCCCAATCCATGTCTCATGGATTAGCATCTCCACGTTCAAAAGATGCTGCAGTTGAGAGTCAGCAACCTGCCGAGGCCTCCGGTGTTGGGAGGAGCTGGGTTCAGAGCATGTTCAGCAGGGATAATTCAACCAGATTAAACTCTTTCAGTCGTGTTCGCAAATGGACTTCTGATGGTGGCTCTTCAGCTACAAATGAAAATGGGACATCTCGTAAGCGAGAATTATCTGCTGCTGGACAGAAGAAAACCCAAACCAATGTTCGAGTACTTAGAGGCCATACTGGCCCTATCACTGCTCTACATTGTGTAACAAGGAGGGAAGTTTGGGATCTAGTGAGTGACCGTGAAGACGCAGGTTTCTTCATCAGTGGGAGCACAGACTGCACGGTTAAGTTTTCAGAGTTAGCTTTGTTTCCTTTCTCTCATTCTTTTGGTTTTGGCCTTATTTGCAGAACCATCCGTGCAATAAGTTCGGATCGAGGAAAGGTGGTATCTGGATCAGATGATCAATCTGTTTTAGTATGGGATAAGCAAACTACTCAGCTTCTAGAAGAGTTGAAAGGCCATGATGCACAGATTAGCTGTGCTCGCATGCTGTCAGGGGAACGTGTCCTTACTGCTGCTCATGATGGAACTGTTAAAATGTGGGATGTTAGAACTGATACTTGTGTGGCAACTGTTGGTCGTTGCTCTAGTGCTGTCCTCTGCATGGAATATGATGACTCCACAGGAATCCTAGCTGCTGGTGGTAGAGATGCCATTGCTAACATTTGGGACATTCGTGCCGGTAGGCAAATGCACAAGCTTCTAGGGCACACAAAATGGATCCGGTCAATTAGAATGGTGGGGGACACAGTGGTTACTGGTAGTGATGATTGGACTGCAAGAATGTGGTCCATTTCCAGAGGAACATGTGACGCTGTTCTTGCATGCCATGCTGGCCCAATATTATCCGTTGAGTACTCGACACTTGACAGAGGAATAATCACAGGTTCAACGGATGGCTTACTTCGCTTTTGGGAAAATGTGGAAGGAGGTATAAGATGTGTAAAAAATGTTACTGTTCACACTGCTGCAATATTGTCCATCAATGCTGCGGAACATTGGTTAGGAATCGGAGCAGCTGATAATTCCATGTCTCTTTTTCATCGGCCTCAAGAGAAACTTGGCGGCTTCTCTAATACGGGATCAAAAATGGCTGGGTGGCAACTATACCGAACACCACAAAGAACAGCTGCTGTGGTAAGATGCGTTGCTACGGACCTTGAGAGAAAAAGGATATGCAGTGGTGATCGCAAGGGCCAGCTTCGGCTATGGGAAGCCACTATAAACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0002682 GO:0002683 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0007049 GO:0007275 GO:0008150 GO:0009791 GO:0009825 GO:0009888 GO:0009987 GO:0010235 GO:0010374 GO:0010440 GO:0016020 GO:0016049 GO:0022402 GO:0030135 GO:0030136 GO:0031347 GO:0031348 GO:0031410 GO:0031461 GO:0031982 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045824 GO:0048519 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0051301 GO:0061640 GO:0065007 GO:0071944 GO:0080008 GO:0080134 GO:0090558 GO:0097708 GO:1902410 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DENN WD40 dDENN uDENN

Protein Analysis

1207

Amino Acids

133.72

Weight (kDa)

5.68

Isoelectric Point (pI)

43.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
uDENN	PF03456	35 - 103	2.2e-12	uDENN domain
DENN	PF02141	123 - 304	1.3e-42	DENN (AEX-3) domain
WD40_Gbeta	PF25391	836 - 1025	4.2e-08	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	845 - 981	6.7e-08	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	883 - 1031	2.5e-20	WDR5 beta-propeller domain
WDR55	PF24796	912 - 1100	1.9e-17	WDR55
WD40_WDHD1_1st	PF24817	917 - 988	3.4e-08	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	917 - 1024	4e-08	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	918 - 989	4.8e-14	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	918 - 1146	2.5e-26	THOC3 beta-propeller domain
WD40_Prp19	PF24814	919 - 995	1.2e-10	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	920 - 1041	8e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	920 - 1067	1.1e-14	CDC20/Fizzy WD40 domain
Beta-prop_WDR41	PF25178	921 - 1014	1.3e-07	WDR41
Beta-prop_WDR36-Utp21_1st	PF25171	924 - 1102	8e-12	WDR36/Utp21 first beta-propeller
Beta-prop_DCAF4	PF23761	951 - 1027	6.3e-07	DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	964 - 1066	2.3e-07	WDR36/Utp21 second beta-propeller domain
WD40_Gbeta	PF25391	966 - 1144	1.8e-14	G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st	PF25173	994 - 1103	2.7e-19	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	995 - 1115	1.2e-14	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	995 - 1203	2.7e-13	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	1009 - 1126	2.2e-18	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	1028 - 1151	5.8e-08	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	1045 - 1118	8.7e-07	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007797)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49040 AT1G49040 AT1G49040 AT1G49040
fragaria_vesca	FvH4_5g12450 FvH4_5g12450
malus_domestica	MD14G1224200.v1.1
prunus_persica	Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1
pyrus_communis	pycom06g19080 pycom14g18580
rosa_chinensis	RchiOBHm_Chr7g0185021
rosa_laevigata	RLG00000004938
rosa_multiflora	Rmu_ssc0000289.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269260
rosa_rugosa	Rorug06G0466200
rosa_samantha	Rh7AG076700 Rh7BG070700 Rh7CG072200 Rh7DG071200
rosa_wichuraiana	Rw7G005890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1415, 2216
Acc36I	ACCTGC	4 cut(s)	108, 952, 2362, 2630
AccB1I	GGYRCC	1 cut(s)	65
AccBSI	CCGCTC	1 cut(s)	1615
AccI	GTMKAC	3 cut(s)	349, 783, 813
AccII	CGCG	1 cut(s)	394
AciI	CCGC	9 cut(s)	33, 129, 155, 158, 394, 1424, 1615, 3383, 3456
AcoI	YGGCCR	1 cut(s)	2076
AcsI	RAATTY	5 cut(s)	358, 662, 1393, 1524, 1810
AcuI	CTGAAG	3 cut(s)	357, 1857, 2454
AcyI	GRCGYC	1 cut(s)	66
AfaI	GTAC	7 cut(s)	98, 426, 513, 633, 2165, 2553, 3248
AfiI	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
AflIII	ACRYGT	2 cut(s)	2893, 3197
AjiI	CACGTC	1 cut(s)	1186
AjnI	CCWGG	2 cut(s)	526, 1573
AjuI	GAANNNNNNNTTGG	4 cut(s)	1066, 1098, 2529, 2561
AleI	CACNNNNGTG	2 cut(s)	176, 674
AloI	GAACNNNNNNTCC	2 cut(s)	896, 928
Alw21I	GWGCWC	4 cut(s)	21, 1806, 2663, 2875
Alw26I	GTCTC	4 cut(s)	410, 572, 2304, 3429
AlwNI	CAGNNNCTG	2 cut(s)	1669, 3524
Ama87I	CYCGRG	1 cut(s)	953
ApoI	RAATTY	5 cut(s)	358, 662, 1393, 1524, 1810
ArsI	GACNNNNNNTTYG	2 cut(s)	1246, 1278
Asp700I	GAANNNNTTC	1 cut(s)	2275
AspLEI	GCGC	2 cut(s)	68, 1218
AsuC2I	CCSGG	1 cut(s)	311
AsuHPI	GGTGA	5 cut(s)	284, 390, 1551, 2107, 3587
AsuNHI	GCTAGC	1 cut(s)	1553
AvaI	CYCGRG	1 cut(s)	953
AvaII	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
AxyI	CCTNAGG	1 cut(s)	208
BaeGI	GKGCMC	1 cut(s)	3102
BaeI	ACNNNNGTAYC	4 cut(s)	361, 394, 2940, 2973
BamHI	GGATCC	2 cut(s)	313, 3111
BanI	GGYRCC	1 cut(s)	65
BanII	GRGCYC	1 cut(s)	600
BbsI	GAAGAC	5 cut(s)	842, 1180, 1673, 2055, 2640
Bbv12I	GWGCWC	4 cut(s)	21, 1806, 2663, 2875
BbvCI	CCTCAGC	2 cut(s)	162, 2222
BceAI	ACGGC	5 cut(s)	79, 88, 97, 610, 659
BcgI	CGANNNNNNTGC	2 cut(s)	2739, 2773
BciT130I	CCWGG	2 cut(s)	528, 1575
BciVI	GTATCC	3 cut(s)	514, 1011, 2195
BclI	TGATCA	2 cut(s)	2101, 2791
BcnI	CCSGG	1 cut(s)	311
BcoDI	GTCTC	4 cut(s)	410, 572, 2304, 3429
BfmI	CTRYAG	3 cut(s)	1533, 1661, 2334
BfoI	RGCGCY	1 cut(s)	69
BfuAI	ACCTGC	4 cut(s)	108, 952, 2362, 2630
BfuI	GTATCC	3 cut(s)	514, 1011, 2195
BglI	GCCNNNNNGGC	2 cut(s)	2568, 3225
BglII	AGATCT	2 cut(s)	9, 1021
BmcAI	AGTACT	2 cut(s)	2553, 3248
Bme1390I	CCNGG	3 cut(s)	311, 528, 1575
Bme18I	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
BmeT110I	CYCGRG	1 cut(s)	953
BmgBI	CACGTC	1 cut(s)	1186
BmiI	GGNNCC	6 cut(s)	67, 315, 599, 1364, 1695, 3113
BmrFI	CCNGG	3 cut(s)	311, 528, 1575
BmrI	ACTGGG	4 cut(s)	1364, 1700, 2147, 2275
BmtI	GCTAGC	1 cut(s)	1557
BmuI	ACTGGG	4 cut(s)	1364, 1700, 2147, 2275
BoxI	GACNNNNGTC	1 cut(s)	561
BpiI	GAAGAC	5 cut(s)	842, 1180, 1673, 2055, 2640
BpmI	CTGGAG	1 cut(s)	449
Bpu10I	CCTNAGC	2 cut(s)	162, 2222
BpuEI	CTTGAG	4 cut(s)	929, 1949, 3426, 3572
BpuMI	CCSGG	1 cut(s)	311
BsaBI	GATNNNNATC	3 cut(s)	698, 1913, 2311
BsaHI	GRCGYC	1 cut(s)	66
BsaJI	CCNNGG	4 cut(s)	79, 242, 1082, 2358
BsaWI	WCCGGW	4 cut(s)	43, 316, 2366, 3114
BsaXI	ACNNNNNCTCC	6 cut(s)	390, 420, 422, 452, 1588, 1618
Bsc4I	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
Bse118I	RCCGGY	3 cut(s)	184, 352, 3071
Bse1I	ACTGG	9 cut(s)	118, 222, 432, 1359, 1695, 2142, 2270, 2572, 3154
Bse21I	CCTNAGG	1 cut(s)	208
Bse3DI	GCAATG	3 cut(s)	300, 888, 3045
Bse8I	GATNNNNATC	3 cut(s)	698, 1913, 2311
BseBI	CCWGG	2 cut(s)	528, 1575
BseDI	CCNNGG	4 cut(s)	79, 242, 1082, 2358
BseGI	GGATG	9 cut(s)	598, 892, 1039, 1823, 1914, 1963, 2742, 2941, 3292
BseJI	GATNNNNATC	3 cut(s)	698, 1913, 2311
BseLI	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
BseMI	GCAATG	3 cut(s)	300, 888, 3045
BseNI	ACTGG	9 cut(s)	118, 222, 432, 1359, 1695, 2142, 2270, 2572, 3154
BseRI	GAGGAG	4 cut(s)	141, 196, 446, 2392
BseSI	GKGCMC	1 cut(s)	3102
BseYI	CCCAGC	2 cut(s)	2382, 3485
BsgI	GTGCAG	1 cut(s)	2653
Bsh1236I	CGCG	1 cut(s)	394
BshNI	GGYRCC	1 cut(s)	65
BsiHKAI	GWGCWC	4 cut(s)	21, 1806, 2663, 2875
BsiHKCI	CYCGRG	1 cut(s)	953
BsiSI	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
BslFI	GGGAC	5 cut(s)	1707, 2154, 2503, 3074, 3149
BslI	CCNNNNNNNGG	6 cut(s)	316, 527, 593, 1070, 2373, 2995
BsmAI	GTCTC	4 cut(s)	410, 572, 2304, 3429
BsmFI	GGGAC	5 cut(s)	1707, 2154, 2503, 3074, 3149
BsmI	GAATGC	1 cut(s)	1202
BsoBI	CYCGRG	1 cut(s)	953
Bsp1286I	GDGCHC	6 cut(s)	21, 600, 1806, 2663, 2875, 3102
BspACI	CCGC	9 cut(s)	33, 129, 155, 158, 394, 1424, 1615, 3383, 3456
BspFNI	CGCG	1 cut(s)	394
BspHI	TCATGA	2 cut(s)	1096, 2911
BspLI	GGNNCC	6 cut(s)	67, 315, 599, 1364, 1695, 3113
BspMAI	CTGCAG	3 cut(s)	1537, 1665, 2338
BspMI	ACCTGC	4 cut(s)	108, 952, 2362, 2630
BspOI	GCTAGC	1 cut(s)	1557
BspQI	GCTCTTC	1 cut(s)	2473
BspT107I	GGYRCC	1 cut(s)	65
BsrBI	CCGCTC	1 cut(s)	1615
BsrDI	GCAATG	3 cut(s)	300, 888, 3045
BsrFI	RCCGGY	3 cut(s)	184, 352, 3071
BsrI	ACTGG	9 cut(s)	118, 222, 432, 1359, 1695, 2142, 2270, 2572, 3154
BssAI	RCCGGY	3 cut(s)	184, 352, 3071
BssECI	CCNNGG	4 cut(s)	79, 242, 1082, 2358
BssNAI	GTATAC	1 cut(s)	350
BssNI	GRCGYC	1 cut(s)	66
BssT1I	CCWWGG	1 cut(s)	1082
Bst1107I	GTATAC	1 cut(s)	350
Bst2UI	CCWGG	2 cut(s)	528, 1575
Bst6I	CTCTTC	3 cut(s)	1494, 2473, 2832
BstACI	GRCGYC	1 cut(s)	66
BstAPI	GCANNNNNTGC	2 cut(s)	2667, 3221
BstC8I	GCNNGC	9 cut(s)	186, 190, 1555, 1617, 2875, 2879, 3217, 3226, 3590
BstDSI	CCRYGG	2 cut(s)	79, 242
BstEII	GGTNACC	1 cut(s)	396
BstF5I	GGATG	9 cut(s)	598, 892, 1039, 1823, 1914, 1963, 2742, 2941, 3292
BstFNI	CGCG	1 cut(s)	394
BstH2I	RGCGCY	1 cut(s)	69
BstHHI	GCGC	2 cut(s)	68, 1218
BstMAI	GTCTC	4 cut(s)	410, 572, 2304, 3429
BstNI	CCWGG	2 cut(s)	528, 1575
BstNSI	RCATGY	5 cut(s)	2243, 2398, 2881, 3201, 3219
BstPAI	GACNNNNGTC	1 cut(s)	561
BstPI	GGTNACC	1 cut(s)	396
BstSCI	CCNGG	3 cut(s)	309, 526, 1573
BstSFI	CTRYAG	3 cut(s)	1533, 1661, 2334
BstSLI	GKGCMC	1 cut(s)	3102
BstUI	CGCG	1 cut(s)	394
BstV2I	GAAGAC	5 cut(s)	842, 1180, 1673, 2055, 2640
BstX2I	RGATCY	6 cut(s)	9, 48, 313, 1021, 2614, 3111
BstXI	CCANNNNNNTGG	2 cut(s)	771, 1012
BstYI	RGATCY	6 cut(s)	9, 48, 313, 1021, 2614, 3111
BstZ17I	GTATAC	1 cut(s)	350
Bsu36I	CCTNAGG	1 cut(s)	208
BsuI	GTATCC	3 cut(s)	514, 1011, 2195
BtgI	CCRYGG	2 cut(s)	79, 242
BtrI	CACGTC	1 cut(s)	1186
BtsCI	GGATG	9 cut(s)	598, 892, 1039, 1823, 1914, 1963, 2742, 2941, 3292
BtsI	GCAGTG	4 cut(s)	1783, 2577, 3354, 3577
BveI	ACCTGC	4 cut(s)	108, 952, 2362, 2630
Cac8I	GCNNGC	9 cut(s)	186, 190, 1555, 1617, 2875, 2879, 3217, 3226, 3590
CaiI	CAGNNNCTG	2 cut(s)	1669, 3524
CciI	TCATGA	2 cut(s)	1096, 2911
CfoI	GCGC	2 cut(s)	68, 1218
Cfr10I	RCCGGY	3 cut(s)	184, 352, 3071
CpoI	CGGWCCG	1 cut(s)	41
CseI	GACGC	6 cut(s)	483, 965, 1667, 1976, 2645, 3212
Csp6I	GTAC	7 cut(s)	97, 425, 512, 632, 2164, 2552, 3247
CspI	CGGWCCG	1 cut(s)	41
CviQI	GTAC	7 cut(s)	97, 425, 512, 632, 2164, 2552, 3247
DinI	GGCGCC	1 cut(s)	67
EaeI	YGGCCR	1 cut(s)	2076
Eam1104I	CTCTTC	3 cut(s)	1494, 2473, 2832
EarI	CTCTTC	3 cut(s)	1494, 2473, 2832
EciI	GGCGGA	1 cut(s)	144
Eco130I	CCWWGG	1 cut(s)	1082
Eco147I	AGGCCT	1 cut(s)	2363
Eco24I	GRGCYC	1 cut(s)	600
Eco47I	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
Eco57I	CTGAAG	3 cut(s)	357, 1857, 2454
Eco81I	CCTNAGG	1 cut(s)	208
Eco88I	CYCGRG	1 cut(s)	953
Eco91I	GGTNACC	1 cut(s)	396
EcoO109I	RGGNCCY	1 cut(s)	1694
EcoO65I	GGTNACC	1 cut(s)	396
EcoRII	CCWGG	2 cut(s)	526, 1573
EcoT14I	CCWWGG	1 cut(s)	1082
EcoT22I	ATGCAT	1 cut(s)	2161
EcoT38I	GRGCYC	1 cut(s)	600
EgeI	GGCGCC	1 cut(s)	67
EheI	GGCGCC	1 cut(s)	67
ErhI	CCWWGG	1 cut(s)	1082
FalI	AAGNNNNNCTT	2 cut(s)	3435, 3467
FaqI	GGGAC	5 cut(s)	1707, 2154, 2503, 3074, 3149
FauI	CCCGC	2 cut(s)	136, 1608
FbaI	TGATCA	2 cut(s)	2101, 2791
FblI	GTMKAC	3 cut(s)	349, 783, 813
FokI	GGATG	9 cut(s)	605, 899, 1046, 1830, 1921, 1970, 2729, 2948, 3299
FriOI	GRGCYC	1 cut(s)	600
GlaI	GCGC	2 cut(s)	67, 1217
GsaI	CCCAGC	2 cut(s)	2386, 3489
GsuI	CTGGAG	1 cut(s)	449
HaeII	RGCGCY	1 cut(s)	69
HapII	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
HgaI	GACGC	6 cut(s)	483, 965, 1667, 1976, 2645, 3212
HhaI	GCGC	2 cut(s)	68, 1218
Hin1I	GRCGYC	1 cut(s)	66
Hin6I	GCGC	2 cut(s)	66, 1216
HinP1I	GCGC	2 cut(s)	66, 1216
HincII	GTYRAC	4 cut(s)	649, 1227, 1254, 1833
HindII	GTYRAC	4 cut(s)	649, 1227, 1254, 1833
HindIII	AAGCTT	2 cut(s)	339, 3089
HinfI	GANTC	9 cut(s)	218, 580, 1268, 1751, 2271, 2344, 3008, 3018, 3399
HpaII	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
HphI	GGTGA	5 cut(s)	284, 390, 1551, 2107, 3587
Hpy99I	CGWCG	4 cut(s)	29, 65, 235, 238
HpyCH4IV	ACGT	7 cut(s)	236, 291, 1185, 1892, 2023, 2320, 2893
HpySE526I	ACGT	7 cut(s)	236, 291, 1185, 1892, 2023, 2320, 2893
Hsp92I	GRCGYC	1 cut(s)	66
HspAI	GCGC	2 cut(s)	66, 1216
KasI	GGCGCC	1 cut(s)	65
KroI	GCCGGC	1 cut(s)	184
KroNI	GCCGGC	1 cut(s)	186
Ksp22I	TGATCA	2 cut(s)	2101, 2791
LguI	GCTCTTC	1 cut(s)	2473
LmnI	GCTCC	8 cut(s)	171, 246, 603, 1612, 1801, 2379, 2658, 3404
MaeII	ACGT	7 cut(s)	236, 291, 1185, 1892, 2023, 2320, 2893
MbiI	CCGCTC	1 cut(s)	1615
MfeI	CAATTG	1 cut(s)	559
MflI	RGATCY	6 cut(s)	9, 48, 313, 1021, 2614, 3111
MhlI	GDGCHC	6 cut(s)	21, 600, 1806, 2663, 2875, 3102
Mly113I	GGCGCC	1 cut(s)	66
MlyI	GAGTC	3 cut(s)	574, 2353, 3002
Mph1103I	ATGCAT	1 cut(s)	2161
MroNI	GCCGGC	1 cut(s)	184
MroXI	GAANNNNTTC	1 cut(s)	2275
MslI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 858
MspA1I	CMGCKG	2 cut(s)	3410, 3521
MspI	CCGG	8 cut(s)	44, 185, 310, 317, 353, 2367, 3072, 3115
MspR9I	CCNGG	3 cut(s)	311, 528, 1575
MunI	CAATTG	1 cut(s)	559
Mva1269I	GAATGC	1 cut(s)	1202
MvaI	CCWGG	2 cut(s)	528, 1575
MvnI	CGCG	1 cut(s)	394
NaeI	GCCGGC	1 cut(s)	186
NarI	GGCGCC	1 cut(s)	66
NciI	CCSGG	1 cut(s)	311
NgoMIV	GCCGGC	1 cut(s)	184
NheI	GCTAGC	1 cut(s)	1553
NlaIV	GGNNCC	6 cut(s)	67, 315, 599, 1364, 1695, 3113
NmeAIII	GCCGAG	1 cut(s)	2383
NmuCI	GTSAC	4 cut(s)	396, 2095, 2624, 3200
NsiI	ATGCAT	1 cut(s)	2161
NspI	RCATGY	5 cut(s)	2243, 2398, 2881, 3201, 3219
OliI	CACNNNNGTG	2 cut(s)	176, 674
PacI	TTAATTAA	1 cut(s)	660
PaeI	GCATGC	2 cut(s)	2881, 3219
PagI	TCATGA	2 cut(s)	1096, 2911
PceI	AGGCCT	1 cut(s)	2363
PciI	ACATGT	1 cut(s)	3197
PciSI	GCTCTTC	1 cut(s)	2473
PcsI	WCGNNNNNNNCGW	2 cut(s)	21, 87
PctI	GAATGC	1 cut(s)	1202
PdiI	GCCGGC	1 cut(s)	186
PdmI	GAANNNNTTC	1 cut(s)	2275
PfeI	GAWTC	6 cut(s)	218, 1268, 1751, 2271, 3018, 3399
PfoI	TCCNGGA	2 cut(s)	309, 526
PleI	GAGTC	3 cut(s)	574, 2352, 3002
PluTI	GGCGCC	1 cut(s)	69
PpsI	GAGTC	3 cut(s)	574, 2352, 3002
PpuMI	RGGWCCY	1 cut(s)	1694
PscI	ACATGT	1 cut(s)	3197
PshAI	GACNNNNGTC	1 cut(s)	561
PsiI	TTATAA	2 cut(s)	1415, 2216
Psp5II	RGGWCCY	1 cut(s)	1694
Psp6I	CCWGG	2 cut(s)	526, 1573
PspEI	GGTNACC	1 cut(s)	396
PspFI	CCCAGC	2 cut(s)	2382, 3485
PspGI	CCWGG	2 cut(s)	526, 1573
PspN4I	GGNNCC	6 cut(s)	67, 315, 599, 1364, 1695, 3113
PspPPI	RGGWCCY	1 cut(s)	1694
PstI	CTGCAG	3 cut(s)	1537, 1665, 2338
PstNI	CAGNNNCTG	2 cut(s)	1669, 3524
PsuI	RGATCY	6 cut(s)	9, 48, 313, 1021, 2614, 3111
PvuII	CAGCTG	2 cut(s)	3410, 3521
RsaI	GTAC	7 cut(s)	98, 426, 513, 633, 2165, 2553, 3248
RsaNI	GTAC	7 cut(s)	97, 425, 512, 632, 2164, 2552, 3247
RseI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 858
Rsr2I	CGGWCCG	1 cut(s)	41
RsrII	CGGWCCG	1 cut(s)	41
SapI	GCTCTTC	1 cut(s)	2473
ScaI	AGTACT	2 cut(s)	2553, 3248
SchI	GAGTC	3 cut(s)	574, 2353, 3002
ScrFI	CCNGG	3 cut(s)	311, 528, 1575
SduI	GDGCHC	6 cut(s)	21, 600, 1806, 2663, 2875, 3102
SfcI	CTRYAG	3 cut(s)	1533, 1661, 2334
SfiI	GGCCNNNNNGGCC	1 cut(s)	2568
SfoI	GGCGCC	1 cut(s)	67
SinI	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
SmiMI	CAYNNNNRTG	4 cut(s)	176, 674, 769, 858
SmlI	CTYRAG	4 cut(s)	908, 1928, 3441, 3551
SmoI	CTYRAG	4 cut(s)	908, 1928, 3441, 3551
SphI	GCATGC	2 cut(s)	2881, 3219
SseBI	AGGCCT	1 cut(s)	2363
SsiI	CCGC	9 cut(s)	33, 129, 155, 158, 394, 1424, 1615, 3383, 3456
SspDI	GGCGCC	1 cut(s)	65
SspI	AATATT	3 cut(s)	716, 3234, 3366
StuI	AGGCCT	1 cut(s)	2363
StyD4I	CCNGG	3 cut(s)	309, 526, 1573
StyI	CCWWGG	1 cut(s)	1082
TaiI	ACGT	7 cut(s)	239, 294, 1188, 1895, 2026, 2323, 2896
TaqI	TCGA	8 cut(s)	15, 60, 114, 538, 555, 2548, 2764, 3251
TaqII	GACCGA	1 cut(s)	1212
TatI	WGTACW	3 cut(s)	424, 2551, 3246
TauI	GCSGC	2 cut(s)	160, 3459
TfiI	GAWTC	6 cut(s)	218, 1268, 1751, 2271, 3018, 3399
TseFI	GTSAC	4 cut(s)	396, 2095, 2624, 3200
Tsp45I	GTSAC	4 cut(s)	396, 2095, 2624, 3200
TspGWI	ACGGA	7 cut(s)	259, 398, 780, 2735, 3229, 3299, 3560
VpaK11BI	GGWCC	5 cut(s)	41, 904, 1694, 3180, 3547
XapI	RAATTY	5 cut(s)	358, 662, 1393, 1524, 1810
XbaI	TCTAGA	2 cut(s)	640, 2833
XceI	RCATGY	5 cut(s)	2243, 2398, 2881, 3201, 3219
XmiI	GTMKAC	3 cut(s)	349, 783, 813
XmnI	GAANNNNTTC	1 cut(s)	2275
ZrmI	AGTACT	2 cut(s)	2553, 3248
Zsp2I	ATGCAT	1 cut(s)	2161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.