Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0015255

Physical Location & Seq

Genomic Coordinates Forward (+)

18622017 .. 18624250

Gene Structure 2234 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G50270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1791 bp

ATGATTGAGCTCAAAACATTGCTTGATCTTCCATTACACTTTCTTCATCTCAAGCAGATCCATTGCTTACTCCTAACTTCTCCGATATTCTATACTCGTCGCGATCTCTTTCTCTCACGTCTTCTCCGTCGCTGCTGCACCGCCGCAACTCAATTCCGCTACGCTCGTCGTTTATTATGTCAACTCCAAACTCTGAGCATTCAACTATGGGATTCGCTCATTGGTCACTTCTCCGGCGGGATTACTCTCAACCGTCGGTTATCATTTCTTGCTTATCGTCATATGCGGCGAAACGGCGTCATCCCTAGCCGCCACACGTTTCCTCCGCTTCTCAAAGCGGTTTTCAAACTGAGAGACTCGAACCCGTTTCAGTTTCATGCTCATATCGTTAAATTCGGGTTGGATTCGGATCCTTTTGTCAGGAATTCGTTGATTTCGGGTTATTCTAGTTCTGGGTTATTCGATTTTGCAAGTCGTCTTTTTGATGGAGCTGAAGATAAGGACGTGGTTACTTGGACTGCAATGATTGATGGGTTTGTGAGAAATGGTTCTGCTTCGGAAGCAATGGTGTATTTCGTGGAGATGAAGAAGACTGGAGTCGCGGCTAACGAAATGACCGTTGTTAGCGTCTTGAAAGCTGCAGGGAAGGTTGAAGATGTTCGATTTGGGAGAAGCGTTCATGGGCTTTACTTAGAAACAGGGAGAGTGAAATGCGATGTGTTCATAGGAAGTTCTCTAGTAGATATGTATGGGAAATGTAGTTGTTATGATGATGCCCAGAAAGTGTTCGATGAAATGCCTAGCAGAAACGTGGTTACTTGGACGGCGTTGATTGCTGGATATGTGCAAAGCCGTTGCTTCGATAAAGGGATGTTGGTTTTCGAGGAGATGTTAAAGAGTGATGTTGCGCCTAATGAGAAGACTCTAAGCAGTGTTCTTTCGGCGTGCGCTCATGTCGGGGCACTTCACCGAGGAAGAAGGGTGCATTGTTATATGATTAAGAATTCGATTGAGATAAACACTACAGCTGGAACTACATTGATTGATCTTTATGTCAAGTGCGGGTGTTTAGAGGAAGCTATACTTGTTTTTGAGAGACTTCATGAGAAAAATGTGTATACTTGGACCGCGATGATTAATGGATTTGCAGCACACGGATATGCTAGAGATGCATTTGATCTCTTTTATACCATGTTGAGTAGTCATGTTTCACCAAATGAAGTTACTTTCATGGCTGTCTTAAGTGCCTGCGCTCATGGAGGTCTTGTGGAAGAGGGTCGAAGGCTTTTCCTGAGCATGAAGGGACGTTTCAATATGGAGCCAAAAGCGGATCACTATGCGTGTATGGTTGATTTATTTGGAAGAAAAGGTTTGCTAGAAGAGGCGAAAGCTTTGATAGAAAGAATGCCAATGGAGCCAACAAATGTGGTATGGGGAGCTCTGTTCGGCTCTTGTTTGTTACACAAAGACTATGAGTTAGGTAAATACGCAGCCTCCCGTGTGATCAAGCTGCAACCTAGTCATAGTGGGAGATACACTCTCTTGGCTAATTTGTACTCGGAATCCCAAAACTGGGATGAAGTCGCTCGGGTAAGAAAGCAAATGAAAGATCAACAGGTGGTGAAATCGCCAGGATTTAGTTGGATCGAAGTGAAAGGCAAGCTTTGTGAATTCATAGCTTTCGATGATAAGAAACCCCTTGAATCTGATGATTTGTACAAGACTCTCGACACTGTTGGGGTGCAAATGAGATTGCCAGATGAATTGGAAGATGTGACTGCTGAATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

596

Amino Acids

67.4

Weight (kDa)

8.35

Isoelectric Point (pI)

43.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	167 - 213	1.8e-09	PPR repeat family
PPR	PF01535	170 - 200	2.7e-07	PPR repeat
PPR_2	PF13041	269 - 318	2.6e-11	PPR repeat family
PPR	PF01535	272 - 301	2.4e-06	PPR repeat
PPR_2	PF13041	370 - 419	5.3e-11	PPR repeat family
E_motif	PF20431	488 - 550	4.2e-19	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50270
fragaria_vesca	FvH4_4g13480
malus_domestica	MD04G1013900.v1.1
prunus_persica	Prupe.1G160900_v2.0.a1
pyrus_communis	pycom04g01080
rosa_chinensis	RchiOBHm_Chr4g0414091
rosa_laevigata	RLG00000008178
rosa_multiflora	Rmu_sc0003264.1_g000012 Rmu_sc0006428.1_g000004
rosa_roxburghii	Rroxscaffold_5G00358020
rosa_rugosa	Rorug04G0125300
rosa_samantha	Rh4AG184000 Rh4BG183500 Rh4CG195900 Rh4DG179400
rosa_wichuraiana	Rw4G015660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1118
AccII	CGCG	3 cut(s)	102, 602, 1130
AclWI	GGATC	5 cut(s)	52, 404, 417, 1338, 1652
AcsI	RAATTY	4 cut(s)	392, 424, 1003, 1670
AcuI	CTGAAG	1 cut(s)	513
AcyI	GRCGYC	1 cut(s)	297
AfaI	GTAC	2 cut(s)	1556, 1718
AfiI	CCNNNNNNNGG	2 cut(s)	1572, 1573
AflII	CTTAAG	1 cut(s)	1240
AflIII	ACRYGT	1 cut(s)	315
AgsI	TTSAA	6 cut(s)	203, 346, 634, 653, 1312, 1703
AjiI	CACGTC	2 cut(s)	119, 505
AjnI	CCWGG	1 cut(s)	1630
Alw21I	GWGCWC	2 cut(s)	12, 1441
Alw26I	GTCTC	2 cut(s)	348, 1090
AlwI	GGATC	5 cut(s)	52, 404, 417, 1338, 1652
Ama87I	CYCGRG	1 cut(s)	1587
ApeKI	GCWGC	6 cut(s)	132, 135, 638, 1148, 1490, 1510
ApoI	RAATTY	4 cut(s)	392, 424, 1003, 1670
AseI	ATTAAT	1 cut(s)	1137
Asp700I	GAANNNNTTC	3 cut(s)	547, 657, 785
AspLEI	GCGC	3 cut(s)	910, 950, 1253
AspS9I	GGNCC	1 cut(s)	1125
AsuHPI	GGTGA	3 cut(s)	959, 1203, 1633
AvaI	CYCGRG	1 cut(s)	1587
AvaII	GGWCC	1 cut(s)	1125
BaeGI	GKGCMC	1 cut(s)	964
BamHI	GGATCC	1 cut(s)	409
BanII	GRGCYC	2 cut(s)	12, 1441
BbsI	GAAGAC	3 cut(s)	113, 596, 926
Bbv12I	GWGCWC	2 cut(s)	12, 1441
BbvI	GCAGC	6 cut(s)	119, 122, 625, 1160, 1497, 1502
BccI	CCATC	2 cut(s)	479, 524
BceAI	ACGGC	3 cut(s)	310, 837, 840
BciT130I	CCWGG	1 cut(s)	1632
BclI	TGATCA	1 cut(s)	1503
BcoDI	GTCTC	2 cut(s)	348, 1090
BfaI	CTAG	7 cut(s)	306, 447, 737, 801, 1164, 1376, 1518
BfmI	CTRYAG	2 cut(s)	639, 1023
BfrI	CTTAAG	1 cut(s)	1240
Bme1390I	CCNGG	1 cut(s)	1632
Bme18I	GGWCC	1 cut(s)	1125
BmeT110I	CYCGRG	1 cut(s)	1587
BmgBI	CACGTC	2 cut(s)	119, 505
BmgT120I	GGNCC	1 cut(s)	1125
BmiI	GGNNCC	3 cut(s)	411, 1320, 1416
BmrFI	CCNGG	1 cut(s)	1632
BmrI	ACTGGG	1 cut(s)	1582
BmsI	GCATC	2 cut(s)	763, 1159
BmuI	ACTGGG	1 cut(s)	1582
BoxI	GACNNNNGTC	1 cut(s)	596
BpiI	GAAGAC	3 cut(s)	113, 596, 926
BplI	GAGNNNNNCTC	2 cut(s)	1522, 1554
BpmI	CTGGAG	1 cut(s)	615
Bpu10I	CCTNAGC	1 cut(s)	1292
BpuEI	CTTGAG	1 cut(s)	35
BsaBI	GATNNNNATC	1 cut(s)	408
BsaHI	GRCGYC	1 cut(s)	297
BsaJI	CCNNGG	1 cut(s)	970
BsaXI	ACNNNNNCTCC	8 cut(s)	108, 138, 307, 337, 1251, 1281, 1478, 1508
Bsc4I	CCNNNNNNNGG	2 cut(s)	1572, 1573
Bse1I	ACTGG	2 cut(s)	598, 1577
Bse3DI	GCAATG	4 cut(s)	17, 61, 528, 570
Bse8I	GATNNNNATC	1 cut(s)	408
BseBI	CCWGG	1 cut(s)	1632
BseDI	CCNNGG	1 cut(s)	970
BseGI	GGATG	3 cut(s)	300, 876, 1582
BseJI	GATNNNNATC	1 cut(s)	408
BseLI	CCNNNNNNNGG	2 cut(s)	1572, 1573
BseMI	GCAATG	4 cut(s)	17, 61, 528, 570
BseMII	CTCAG	3 cut(s)	185, 341, 1283
BseNI	ACTGG	2 cut(s)	598, 1577
BseRI	GAGGAG	1 cut(s)	899
BseSI	GKGCMC	1 cut(s)	964
BseXI	GCAGC	6 cut(s)	119, 122, 625, 1160, 1497, 1502
BsgI	GTGCAG	1 cut(s)	121
Bsh1236I	CGCG	3 cut(s)	102, 602, 1130
BsiHKAI	GWGCWC	2 cut(s)	12, 1441
BsiHKCI	CYCGRG	1 cut(s)	1587
BsiSI	CCGG	1 cut(s)	234
BslFI	GGGAC	1 cut(s)	1317
BslI	CCNNNNNNNGG	2 cut(s)	1572, 1573
BsmAI	GTCTC	2 cut(s)	348, 1090
BsmFI	GGGAC	1 cut(s)	1317
BsmI	GAATGC	2 cut(s)	198, 1410
BsoBI	CYCGRG	1 cut(s)	1587
Bsp1286I	GDGCHC	3 cut(s)	12, 964, 1441
Bsp1407I	TGTACA	1 cut(s)	1716
Bsp68I	TCGCGA	1 cut(s)	102
BspCNI	CTCAG	3 cut(s)	186, 342, 1284
BspFNI	CGCG	3 cut(s)	102, 602, 1130
BspHI	TCATGA	2 cut(s)	1102, 1787
BspLI	GGNNCC	3 cut(s)	411, 1320, 1416
BspMAI	CTGCAG	1 cut(s)	643
BspPI	GGATC	5 cut(s)	52, 404, 417, 1338, 1652
BspTI	CTTAAG	1 cut(s)	1240
BsrDI	GCAATG	4 cut(s)	17, 61, 528, 570
BsrGI	TGTACA	1 cut(s)	1716
BsrI	ACTGG	2 cut(s)	598, 1577
BssECI	CCNNGG	1 cut(s)	970
BssNAI	GTATAC	1 cut(s)	1119
BssNI	GRCGYC	1 cut(s)	297
Bst1107I	GTATAC	1 cut(s)	1119
Bst2UI	CCWGG	1 cut(s)	1632
Bst4CI	ACNGT	3 cut(s)	254, 619, 1735
Bst6I	CTCTTC	2 cut(s)	1266, 1374
BstACI	GRCGYC	1 cut(s)	297
BstAFI	CTTAAG	1 cut(s)	1240
BstAUI	TGTACA	1 cut(s)	1716
BstC8I	GCNNGC	3 cut(s)	946, 1249, 1661
BstDEI	CTNAG	5 cut(s)	194, 350, 691, 926, 1292
BstF5I	GGATG	3 cut(s)	300, 876, 1582
BstFNI	CGCG	3 cut(s)	102, 602, 1130
BstHHI	GCGC	3 cut(s)	910, 950, 1253
BstMAI	GTCTC	2 cut(s)	348, 1090
BstMWI	GCNNNNNNNGC	4 cut(s)	560, 833, 1169, 1414
BstNI	CCWGG	1 cut(s)	1632
BstPAI	GACNNNNGTC	1 cut(s)	596
BstSCI	CCNGG	1 cut(s)	1630
BstSFI	CTRYAG	2 cut(s)	639, 1023
BstSLI	GKGCMC	1 cut(s)	964
BstUI	CGCG	3 cut(s)	102, 602, 1130
BstV1I	GCAGC	6 cut(s)	119, 122, 625, 1160, 1497, 1502
BstV2I	GAAGAC	3 cut(s)	113, 596, 926
BstX2I	RGATCY	2 cut(s)	57, 409
BstYI	RGATCY	2 cut(s)	57, 409
BstZ17I	GTATAC	1 cut(s)	1119
BtgZI	GCGATG	2 cut(s)	729, 1145
BtrI	CACGTC	2 cut(s)	119, 505
BtsCI	GGATG	3 cut(s)	300, 876, 1582
BtsI	GCAGTG	1 cut(s)	937
BtsIMutI	CAGTG	2 cut(s)	937, 1731
BtuMI	TCGCGA	1 cut(s)	102
Cac8I	GCNNGC	3 cut(s)	946, 1249, 1661
CciI	TCATGA	2 cut(s)	1102, 1787
CfoI	GCGC	3 cut(s)	910, 950, 1253
Cfr13I	GGNCC	1 cut(s)	1125
CseI	GACGC	2 cut(s)	286, 616
Csp6I	GTAC	2 cut(s)	1555, 1717
CspCI	CAANNNNNGTGG	2 cut(s)	1407, 1442
CviQI	GTAC	2 cut(s)	1555, 1717
DdeI	CTNAG	5 cut(s)	194, 350, 691, 926, 1292
Eam1104I	CTCTTC	2 cut(s)	1266, 1374
EarI	CTCTTC	2 cut(s)	1266, 1374
Ecl136II	GAGCTC	2 cut(s)	10, 1439
Eco24I	GRGCYC	2 cut(s)	12, 1441
Eco47I	GGWCC	1 cut(s)	1125
Eco53kI	GAGCTC	2 cut(s)	10, 1439
Eco57I	CTGAAG	1 cut(s)	513
Eco88I	CYCGRG	1 cut(s)	1587
EcoICRI	GAGCTC	2 cut(s)	10, 1439
EcoRI	GAATTC	3 cut(s)	424, 1003, 1670
EcoRII	CCWGG	1 cut(s)	1630
EcoT22I	ATGCAT	1 cut(s)	1174
EcoT38I	GRGCYC	2 cut(s)	12, 1441
FalI	AAGNNNNNCTT	4 cut(s)	1068, 1100, 1647, 1679
FaqI	GGGAC	1 cut(s)	1317
FauI	CCCGC	2 cut(s)	230, 1055
FauNDI	CATATG	1 cut(s)	282
FbaI	TGATCA	1 cut(s)	1503
FblI	GTMKAC	1 cut(s)	1118
FokI	GGATG	3 cut(s)	287, 883, 1589
FriOI	GRGCYC	2 cut(s)	12, 1441
FspBI	CTAG	7 cut(s)	306, 447, 737, 801, 1164, 1376, 1518
GlaI	GCGC	3 cut(s)	909, 949, 1252
GsuI	CTGGAG	1 cut(s)	615
HapII	CCGG	1 cut(s)	234
HgaI	GACGC	2 cut(s)	286, 616
HhaI	GCGC	3 cut(s)	910, 950, 1253
Hin1I	GRCGYC	1 cut(s)	297
Hin6I	GCGC	3 cut(s)	908, 948, 1251
HinP1I	GCGC	3 cut(s)	908, 948, 1251
HincII	GTYRAC	1 cut(s)	182
HindII	GTYRAC	1 cut(s)	182
HindIII	AAGCTT	2 cut(s)	1389, 1661
HinfI	GANTC	9 cut(s)	212, 356, 404, 597, 922, 1562, 1703, 1723, 1784
HpaII	CCGG	1 cut(s)	234
HphI	GGTGA	3 cut(s)	959, 1203, 1633
Hpy166II	GTNNAC	2 cut(s)	182, 1119
Hpy188I	TCNGA	6 cut(s)	84, 195, 409, 559, 1561, 1708
Hpy188III	TCNNGA	7 cut(s)	101, 421, 631, 1103, 1291, 1727, 1788
Hpy8I	GTNNAC	2 cut(s)	182, 1119
Hpy99I	CGWCG	4 cut(s)	102, 132, 171, 258
HpyAV	CCTTC	4 cut(s)	640, 972, 1275, 1294
HpyCH4III	ACNGT	3 cut(s)	254, 619, 1735
HpyCH4IV	ACGT	5 cut(s)	118, 317, 504, 810, 1306
HpyF10VI	GCNNNNNNNGC	4 cut(s)	560, 833, 1169, 1414
HpyF3I	CTNAG	5 cut(s)	194, 350, 691, 926, 1292
HpySE526I	ACGT	5 cut(s)	118, 317, 504, 810, 1306
Hsp92I	GRCGYC	1 cut(s)	297
HspAI	GCGC	3 cut(s)	908, 948, 1251
Ksp22I	TGATCA	1 cut(s)	1503
LmnI	GCTCC	4 cut(s)	488, 1318, 1414, 1436
Lsp1109I	GCAGC	6 cut(s)	119, 122, 625, 1160, 1497, 1502
LweI	GCATC	2 cut(s)	763, 1159
MaeI	CTAG	7 cut(s)	306, 447, 737, 801, 1164, 1376, 1518
MaeII	ACGT	5 cut(s)	118, 317, 504, 810, 1306
MaeIII	GTNAC	6 cut(s)	224, 508, 814, 1222, 1458, 1774
MflI	RGATCY	2 cut(s)	57, 409
MhlI	GDGCHC	3 cut(s)	12, 964, 1441
MluCI	AATT	7 cut(s)	152, 392, 424, 1003, 1549, 1670, 1763
MlyI	GAGTC	4 cut(s)	350, 606, 916, 1717
MmeI	TCCRAC	2 cut(s)	381, 1622
MnlI	CCTC	8 cut(s)	333, 877, 965, 1066, 1253, 1267, 1375, 1504
Mph1103I	ATGCAT	1 cut(s)	1174
MroXI	GAANNNNTTC	3 cut(s)	547, 657, 785
MseI	TTAA	5 cut(s)	390, 893, 999, 1137, 1241
MslI	CAYNNNNRTG	1 cut(s)	1158
MspA1I	CMGCKG	1 cut(s)	1028
MspCI	CTTAAG	1 cut(s)	1240
MspI	CCGG	1 cut(s)	234
MspR9I	CCNGG	1 cut(s)	1632
Mva1269I	GAATGC	2 cut(s)	198, 1410
MvaI	CCWGG	1 cut(s)	1632
MvnI	CGCG	3 cut(s)	102, 602, 1130
MwoI	GCNNNNNNNGC	4 cut(s)	560, 833, 1169, 1414
NdeI	CATATG	1 cut(s)	282
NlaIV	GGNNCC	3 cut(s)	411, 1320, 1416
NmuCI	GTSAC	2 cut(s)	224, 1774
NruI	TCGCGA	1 cut(s)	102
NsiI	ATGCAT	1 cut(s)	1174
PagI	TCATGA	2 cut(s)	1102, 1787
PcsI	WCGNNNNNNNCGW	3 cut(s)	124, 606, 615
PctI	GAATGC	2 cut(s)	198, 1410
PdmI	GAANNNNTTC	3 cut(s)	547, 657, 785
PfeI	GAWTC	5 cut(s)	212, 404, 1562, 1703, 1784
PleI	GAGTC	4 cut(s)	350, 605, 916, 1717
PpsI	GAGTC	4 cut(s)	350, 605, 916, 1717
PshAI	GACNNNNGTC	1 cut(s)	596
PshBI	ATTAAT	1 cut(s)	1137
Psp124BI	GAGCTC	2 cut(s)	12, 1441
Psp6I	CCWGG	1 cut(s)	1630
PspGI	CCWGG	1 cut(s)	1630
PspN4I	GGNNCC	3 cut(s)	411, 1320, 1416
PspPI	GGNCC	1 cut(s)	1125
PstI	CTGCAG	1 cut(s)	643
PsuI	RGATCY	2 cut(s)	57, 409
PvuII	CAGCTG	1 cut(s)	1028
RruI	TCGCGA	1 cut(s)	102
RsaI	GTAC	2 cut(s)	1556, 1718
RsaNI	GTAC	2 cut(s)	1555, 1717
RseI	CAYNNNNRTG	1 cut(s)	1158
SacI	GAGCTC	2 cut(s)	12, 1441
SaqAI	TTAA	5 cut(s)	390, 893, 999, 1137, 1241
Sau96I	GGNCC	1 cut(s)	1125
SchI	GAGTC	4 cut(s)	350, 606, 916, 1717
ScrFI	CCNGG	1 cut(s)	1632
SduI	GDGCHC	3 cut(s)	12, 964, 1441
SfaNI	GCATC	2 cut(s)	763, 1159
SfcI	CTRYAG	2 cut(s)	639, 1023
SinI	GGWCC	1 cut(s)	1125
SmiMI	CAYNNNNRTG	1 cut(s)	1158
SmlI	CTYRAG	2 cut(s)	50, 1240
SmoI	CTYRAG	2 cut(s)	50, 1240
Sse9I	AATT	7 cut(s)	152, 392, 424, 1003, 1549, 1670, 1763
SspMI	CTAG	7 cut(s)	306, 447, 737, 801, 1164, 1376, 1518
SstI	GAGCTC	2 cut(s)	12, 1441
StyD4I	CCNGG	1 cut(s)	1630
TaaI	ACNGT	3 cut(s)	254, 619, 1735
TaiI	ACGT	5 cut(s)	121, 320, 507, 813, 1309
TasI	AATT	7 cut(s)	152, 392, 424, 1003, 1549, 1670, 1763
TatI	WGTACW	2 cut(s)	1554, 1716
TauI	GCSGC	4 cut(s)	146, 289, 312, 605
TfiI	GAWTC	5 cut(s)	212, 404, 1562, 1703, 1784
Tru1I	TTAA	5 cut(s)	390, 893, 999, 1137, 1241
Tru9I	TTAA	5 cut(s)	390, 893, 999, 1137, 1241
TscAI	CASTG	2 cut(s)	937, 1738
TseFI	GTSAC	2 cut(s)	224, 1774
TseI	GCWGC	6 cut(s)	132, 135, 638, 1148, 1490, 1510
Tsp45I	GTSAC	2 cut(s)	224, 1774
TspGWI	ACGGA	2 cut(s)	116, 1170
TspRI	CASTG	2 cut(s)	937, 1738
Vha464I	CTTAAG	1 cut(s)	1240
VpaK11BI	GGWCC	1 cut(s)	1125
VspI	ATTAAT	1 cut(s)	1137
XapI	RAATTY	4 cut(s)	392, 424, 1003, 1670
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1638
XmiI	GTMKAC	1 cut(s)	1118
XmnI	GAANNNNTTC	3 cut(s)	547, 657, 785
XspI	CTAG	7 cut(s)	306, 447, 737, 801, 1164, 1376, 1518
Zsp2I	ATGCAT	1 cut(s)	1174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.