RLG00000008178

Pentatricopeptide repeat domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015255
Physical Location & Seq
Forward (+)
26667485 .. 26669590
2106 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008178

Sequence Viewer

Length: 1791 bp
ATGGGTAGCACCATCTTGTCTCTGTGCCACAAAGACACAACCTTTAACCATTTGAAGCAAATTCACTCCCTCCTCGTTACCCTTGGCCCATCACAAAACGATTGCTTGCTCCCCAAGCTTCTCACTTCCATTGCACCCACCTCTAAGCCTACCCATCTTGCCTATGCTTCCTTCCTCATTGACCATGTACAAGCACCCAACCCCACCCACGTATGGAACTCCATCATCAGAGCATTTTCTACCACTTCAGAGCCTCACAGGTCCCTTGTTTCGTATTCAACAATGCGCCGAAACGGGGTCGTTCCGGACCAGCACACTTTCCCATTGCTACTCAAGGCCTTTCCCAAGTTGAAAAACAAAGACCCATTTCAGTTTTATGCTCATACGGTGAAATTTGGATTGAATTTGGATCAGTTTGTGGGGAATTCGTTTATATCTGTGTTGTCTGGTTGTGGGTACTTGGAGGCTGCGCATCAGGTGTTTGTTGAAAGTACTCAGAAGGATGTGATTGGTTGGACGGCGTTGGTTGATGGGTATGTTAAGAATGGTCATGCTTTGGAAGCAATGAAGTGTTTTGTGGAGATGGGATTGGTTGGAATTAGTGTCGATGAGGTGACTGTTGTTAGTGCTCTTTGTGCTGCTGGAAATGCCGGTGATGTTTGGTTCGGGCGGTGCGTTCATGGGTTCTATGTGGAAGCAGGCAGAGTAAAATGGGATGTCTATGTTGGTAGTGCTCTTGTTGATATGTACATGAAGTGTGGATGCCATGACGATGCTCGAAAAGTGTTTAATGCCATGCCGAGTAAAAATGTGGTATCTTGGACTGCGTTGATAGCAGGTTATGTGCAATGTGAGAAATATAAAGATGCACTGCTTGTCTTGAAATGTATGGTTTCGGAGGATGTCAAGCCTAACCAATCTACATTGTCTAGTGTACTTACCTCTTGTGCGCACGTAGGGGCATTGGATCAAGGAAAGTTGATCCATGGCTATCTATATAGGCATAGAGTACAAGTGAACATGTTGCTTGGAACAGGGTTAGTAGATATGTACGCAAAATGTGGGTGCATTGTTAAGGCTCTATCGGTTTTCAGCAAGCTGCCCAGCAAGGATGTCTTTACATGGACTGCTATGATTAATGGGTTGGCATCACATGGGGATGCTTTAGGCGCTTTGAACTTACTCTCACGGATGTTACAGAATGGGGTTCTACCCAATGAAGTAACCTTCATGGGTGTTCTTAGTGCGTGTGCTCATAGAGGCCTTGTAGATGAGGGACGCAAGGCTTTTGGATCAATGAAGCAAGTTTTCCATTTGGAGCCGAATGTAGATCACTACGGCTGTATGGTTGATTTACTTGGTAGGGCTGGGTACTTGGAGGAAGCCAGACAAATGATTGAGGACATGCCAATGTTGCCTACAGCTGGTATATGGGGAACTTTCCTTAGTGCGTGTGTGATTCACAAGAATTTTGAGCTAGGTGAACTTGCAGGAAGTCATTTGATCAAGCTAGATTCCAATCACAGTGGAAGATACGTGCTTCTGGCAAACTTGTATTCAACTTGGAAAAAATGGAAAGTAGCTGCTGGCATCAGGAAGCTCATGAAAGGGAAAGGCGTTGAAAAAATACCAGGGTATAGCTGGATAGAAGTCAATGGTGCAATGTTTGAGTTCGCTGCGTTTGACAAATCACATTCCGAAACAGATCATATATATCTGATGTTGGAAAACATGCTTGTGCAGTTGAGAGTCTCTGGTTATGTTCCCGACGCTAACCTACTAGCATTTTGA

Protein Analysis

597

Amino Acids

65.63

Weight (kDa)

8.22

Isoelectric Point (pI)

24.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 167 - 207 1.2e-06 PPR repeat family
PPR_2 PF13041 269 - 318 3e-09 PPR repeat family
PPR_2 PF13041 370 - 419 1.9e-10 PPR repeat family
PPR PF01535 373 - 403 3.6e-06 PPR repeat
E_motif PF20431 488 - 550 3.9e-18 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015255)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50270
fragaria_vesca FvH4_4g13480
malus_domestica MD04G1013900.v1.1
prunus_persica Prupe.1G160900_v2.0.a1
pyrus_communis pycom04g01080
rosa_chinensis RchiOBHm_Chr4g0414091
rosa_laevigata RLG00000008178
rosa_multiflora Rmu_sc0003264.1_g000012 Rmu_sc0006428.1_g000004
rosa_roxburghii Rroxscaffold_5G00358020
rosa_rugosa Rorug04G0125300
rosa_samantha Rh4AG184000 Rh4BG183500 Rh4CG195900 Rh4DG179400
rosa_wichuraiana Rw4G015660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 471, 951
Acc36I ACCTGC 1 cut(s) 827
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 1 cut(s) 670
AclWI GGATC 4 cut(s) 417, 975, 976, 1300
AcsI RAATTY 5 cut(s) 60, 392, 403, 424, 1468
AcuI CTGAAG 1 cut(s) 231
AfaI GTAC 8 cut(s) 189, 458, 493, 749, 936, 1011, 1052, 1373
AfiI CCNNNNNNNGG 3 cut(s) 213, 295, 1424
AflIII ACRYGT 1 cut(s) 1020
AgsI TTSAA 9 cut(s) 55, 279, 352, 403, 488, 883, 1177, 1560, 1622
AjnI CCWGG 1 cut(s) 1630
AluBI AGCT 8 cut(s) 118, 1099, 1424, 1477, 1510, 1583, 1600, 1641
AluI AGCT 8 cut(s) 118, 1099, 1424, 1477, 1510, 1583, 1600, 1641
Alw21I GWGCWC 3 cut(s) 631, 736, 1255
Alw26I GTCTC 2 cut(s) 24, 1756
AlwI GGATC 4 cut(s) 417, 975, 976, 1300
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 3 cut(s) 85, 336, 1261
ApeKI GCWGC 5 cut(s) 467, 638, 1099, 1583, 1676
ApoI RAATTY 5 cut(s) 60, 392, 403, 424, 1468
AseI ATTAAT 1 cut(s) 1137
AspLEI GCGC 4 cut(s) 288, 472, 952, 1172
AspS9I GGNCC 3 cut(s) 86, 261, 307
AsuHPI GGTGA 4 cut(s) 400, 625, 665, 1493
AvaII GGWCC 2 cut(s) 261, 307
Bbv12I GWGCWC 3 cut(s) 631, 736, 1255
BbvI GCAGC 5 cut(s) 454, 625, 1086, 1570, 1663
BccI CCATC 6 cut(s) 20, 97, 162, 230, 524, 577
BceAI ACGGC 2 cut(s) 534, 1354
BciT130I CCWGG 1 cut(s) 1632
BclI TGATCA 1 cut(s) 1503
BcoDI GTCTC 2 cut(s) 24, 1756
BfaI CTAG 4 cut(s) 930, 1478, 1511, 1781
BfmI CTRYAG 1 cut(s) 1419
BfoI RGCGCY 1 cut(s) 1173
BfuAI ACCTGC 1 cut(s) 827
BisI GCNGC 5 cut(s) 468, 639, 1100, 1584, 1677
BlsI GCNGC 5 cut(s) 469, 640, 1101, 1585, 1678
BmcAI AGTACT 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 1632
Bme18I GGWCC 2 cut(s) 261, 307
BmgT120I GGNCC 3 cut(s) 86, 261, 307
BmiI GGNNCC 2 cut(s) 263, 1320
BmrFI CCNGG 1 cut(s) 1632
BmsI GCATC 7 cut(s) 481, 752, 763, 856, 1150, 1157, 1599
BpuEI CTTGAG 1 cut(s) 317
BsaAI YACGTR 3 cut(s) 211, 955, 1537
BsaBI GATNNNNATC 1 cut(s) 1518
BsaJI CCNNGG 3 cut(s) 82, 985, 1631
BsaWI WCCGGW 1 cut(s) 304
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 295, 1424
Bse118I RCCGGY 1 cut(s) 650
Bse3DI GCAATG 5 cut(s) 129, 323, 570, 854, 1668
Bse8I GATNNNNATC 1 cut(s) 1518
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 1 cut(s) 1632
BseDI CCNNGG 3 cut(s) 82, 985, 1631
BseGI GGATG 7 cut(s) 508, 721, 767, 907, 1117, 1165, 1197
BseJI GATNNNNATC 1 cut(s) 1518
BseLI CCNNNNNNNGG 3 cut(s) 213, 295, 1424
BseMI GCAATG 5 cut(s) 129, 323, 570, 854, 1668
BseMII CTCAG 1 cut(s) 509
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 5 cut(s) 454, 625, 1086, 1570, 1663
BseYI CCCAGC 2 cut(s) 1103, 1367
BsgI GTGCAG 1 cut(s) 1760
BshFI GGCC 3 cut(s) 87, 338, 1263
BsiHKAI GWGCWC 3 cut(s) 631, 736, 1255
BsiSI CCGG 2 cut(s) 305, 651
BslFI GGGAC 2 cut(s) 247, 1290
BslI CCNNNNNNNGG 3 cut(s) 213, 295, 1424
BsmAI GTCTC 2 cut(s) 24, 1756
BsmFI GGGAC 2 cut(s) 247, 1290
BsnI GGCC 3 cut(s) 87, 338, 1263
Bsp1286I GDGCHC 3 cut(s) 631, 736, 1255
Bsp13I TCCGGA 1 cut(s) 304
Bsp1407I TGTACA 2 cut(s) 187, 747
Bsp143I GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
Bsp19I CCATGG 1 cut(s) 985
BspACI CCGC 1 cut(s) 670
BspANI GGCC 3 cut(s) 87, 338, 1263
BspCNI CTCAG 1 cut(s) 508
BspEI TCCGGA 1 cut(s) 304
BspHI TCATGA 1 cut(s) 1602
BspLI GGNNCC 2 cut(s) 263, 1320
BspMI ACCTGC 1 cut(s) 827
BspPI GGATC 4 cut(s) 417, 975, 976, 1300
BsrDI GCAATG 5 cut(s) 129, 323, 570, 854, 1668
BsrFI RCCGGY 1 cut(s) 650
BsrGI TGTACA 2 cut(s) 187, 747
BssAI RCCGGY 1 cut(s) 650
BssECI CCNNGG 3 cut(s) 82, 985, 1631
BssMI GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
BssT1I CCWWGG 2 cut(s) 82, 985
Bst2UI CCWGG 1 cut(s) 1632
Bst4CI ACNGT 3 cut(s) 388, 619, 1526
BstAUI TGTACA 2 cut(s) 187, 747
BstBAI YACGTR 3 cut(s) 211, 955, 1537
BstC8I GCNNGC 4 cut(s) 107, 700, 1097, 1588
BstDEI CTNAG 4 cut(s) 144, 495, 1241, 1445
BstDSI CCRYGG 1 cut(s) 985
BstF5I GGATG 7 cut(s) 508, 721, 767, 907, 1117, 1165, 1197
BstH2I RGCGCY 1 cut(s) 1173
BstHHI GCGC 4 cut(s) 288, 472, 952, 1172
BstKTI GATC 7 cut(s) 412, 970, 984, 1295, 1333, 1506, 1708
BstMAI GTCTC 2 cut(s) 24, 1756
BstMBI GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
BstMWI GCNNNNNNNGC 7 cut(s) 115, 560, 635, 647, 833, 1169, 1414
BstNI CCWGG 1 cut(s) 1632
BstNSI RCATGY 3 cut(s) 1024, 1408, 1735
BstSCI CCNGG 1 cut(s) 1630
BstSFI CTRYAG 1 cut(s) 1419
BstV1I GCAGC 5 cut(s) 454, 625, 1086, 1570, 1663
BsuRI GGCC 3 cut(s) 87, 338, 1263
BtgI CCRYGG 1 cut(s) 985
BtsCI GGATG 7 cut(s) 508, 721, 767, 907, 1117, 1165, 1197
BtsI GCAGTG 1 cut(s) 869
BtsIMutI CAGTG 2 cut(s) 869, 1531
BveI ACCTGC 1 cut(s) 827
Cac8I GCNNGC 4 cut(s) 107, 700, 1097, 1588
CciI TCATGA 1 cut(s) 1602
CfoI GCGC 4 cut(s) 288, 472, 952, 1172
Cfr10I RCCGGY 1 cut(s) 650
Cfr13I GGNCC 3 cut(s) 86, 261, 307
CseI GACGC 2 cut(s) 1287, 1778
Csp6I GTAC 8 cut(s) 188, 457, 492, 748, 935, 1010, 1051, 1372
CspCI CAANNNNNGTGG 2 cut(s) 1507, 1542
CviQI GTAC 8 cut(s) 188, 457, 492, 748, 935, 1010, 1051, 1372
DdeI CTNAG 4 cut(s) 144, 495, 1241, 1445
DpnI GATC 7 cut(s) 411, 969, 983, 1294, 1332, 1505, 1707
DpnII GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
Eco130I CCWWGG 2 cut(s) 82, 985
Eco147I AGGCCT 2 cut(s) 338, 1263
Eco47I GGWCC 2 cut(s) 261, 307
Eco57I CTGAAG 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 261
EcoRI GAATTC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 1630
EcoT14I CCWWGG 2 cut(s) 82, 985
ErhI CCWWGG 2 cut(s) 82, 985
FalI AAGNNNNNCTT 2 cut(s) 1100, 1132
FaqI GGGAC 2 cut(s) 247, 1290
FbaI TGATCA 1 cut(s) 1503
Fnu4HI GCNGC 5 cut(s) 468, 639, 1100, 1584, 1677
FokI GGATG 7 cut(s) 515, 728, 774, 914, 1124, 1172, 1204
Fsp4HI GCNGC 5 cut(s) 468, 639, 1100, 1584, 1677
FspAI RTGCGCAY 1 cut(s) 951
FspBI CTAG 4 cut(s) 930, 1478, 1511, 1781
FspI TGCGCA 2 cut(s) 471, 951
GlaI GCGC 4 cut(s) 287, 471, 951, 1171
GluI GCNGC 5 cut(s) 468, 639, 1100, 1584, 1677
GsaI CCCAGC 2 cut(s) 1107, 1371
HaeII RGCGCY 1 cut(s) 1173
HaeIII GGCC 3 cut(s) 87, 338, 1263
HapII CCGG 2 cut(s) 305, 651
HgaI GACGC 2 cut(s) 1287, 1778
HhaI GCGC 4 cut(s) 288, 472, 952, 1172
Hin6I GCGC 4 cut(s) 286, 470, 950, 1170
HinP1I GCGC 4 cut(s) 286, 470, 950, 1170
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 3 cut(s) 1459, 1514, 1749
HpaII CCGG 2 cut(s) 305, 651
HphI GGTGA 4 cut(s) 400, 625, 665, 1493
Hpy166II GTNNAC 3 cut(s) 935, 1018, 1484
Hpy188I TCNGA 6 cut(s) 230, 250, 498, 898, 1699, 1719
Hpy188III TCNNGA 5 cut(s) 305, 880, 1594, 1603, 1766
Hpy8I GTNNAC 3 cut(s) 935, 1018, 1484
Hpy99I CGWCG 1 cut(s) 1772
HpyAV CCTTC 3 cut(s) 181, 493, 1237
HpyCH4III ACNGT 3 cut(s) 388, 619, 1526
HpyCH4IV ACGT 3 cut(s) 210, 954, 1536
HpyCH4V TGCA 7 cut(s) 134, 847, 869, 1068, 1490, 1661, 1741
HpyF10VI GCNNNNNNNGC 7 cut(s) 115, 560, 635, 647, 833, 1169, 1414
HpyF3I CTNAG 4 cut(s) 144, 495, 1241, 1445
HpySE526I ACGT 3 cut(s) 210, 954, 1536
HspAI GCGC 4 cut(s) 286, 470, 950, 1170
Kpn2I TCCGGA 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 1503
Kzo9I GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
LmnI GCTCC 2 cut(s) 114, 1318
Lsp1109I GCAGC 5 cut(s) 454, 625, 1086, 1570, 1663
LweI GCATC 7 cut(s) 481, 752, 763, 856, 1150, 1157, 1599
MaeI CTAG 4 cut(s) 930, 1478, 1511, 1781
MaeII ACGT 3 cut(s) 210, 954, 1536
MaeIII GTNAC 4 cut(s) 76, 613, 1194, 1222
MalI GATC 7 cut(s) 411, 969, 983, 1294, 1332, 1505, 1707
MboI GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
MboII GAAGA 1 cut(s) 1542
MhlI GDGCHC 3 cut(s) 631, 736, 1255
MluCI AATT 6 cut(s) 60, 392, 403, 424, 597, 1468
MlyI GAGTC 1 cut(s) 1758
MmeI TCCRAC 3 cut(s) 494, 574, 1704
MroI TCCGGA 1 cut(s) 304
MseI TTAA 5 cut(s) 45, 540, 789, 1074, 1137
MslI CAYNNNNRTG 4 cut(s) 771, 1158, 1409, 1736
MspA1I CMGCKG 1 cut(s) 1424
MspI CCGG 2 cut(s) 305, 651
MspR9I CCNGG 1 cut(s) 1632
MvaI CCWGG 1 cut(s) 1632
MwoI GCNNNNNNNGC 7 cut(s) 115, 560, 635, 647, 833, 1169, 1414
NcoI CCATGG 1 cut(s) 985
NdeII GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
NlaIV GGNNCC 2 cut(s) 263, 1320
NmeAIII GCCGAG 1 cut(s) 825
NmuCI GTSAC 1 cut(s) 613
NsbI TGCGCA 2 cut(s) 471, 951
NspI RCATGY 3 cut(s) 1024, 1408, 1735
PagI TCATGA 1 cut(s) 1602
PceI AGGCCT 2 cut(s) 338, 1263
PciI ACATGT 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PfeI GAWTC 2 cut(s) 1459, 1514
PkrI GCNGC 5 cut(s) 469, 640, 1101, 1585, 1678
PleI GAGTC 1 cut(s) 1757
PpsI GAGTC 1 cut(s) 1757
Ppu21I YACGTR 3 cut(s) 211, 955, 1537
PpuMI RGGWCCY 1 cut(s) 261
PscI ACATGT 1 cut(s) 1020
PshBI ATTAAT 1 cut(s) 1137
Psp5II RGGWCCY 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 1630
PspFI CCCAGC 2 cut(s) 1103, 1367
PspGI CCWGG 1 cut(s) 1630
PspN4I GGNNCC 2 cut(s) 263, 1320
PspPI GGNCC 3 cut(s) 86, 261, 307
PspPPI RGGWCCY 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 1424
RsaI GTAC 8 cut(s) 189, 458, 493, 749, 936, 1011, 1052, 1373
RsaNI GTAC 8 cut(s) 188, 457, 492, 748, 935, 1010, 1051, 1372
RseI CAYNNNNRTG 4 cut(s) 771, 1158, 1409, 1736
SaqAI TTAA 5 cut(s) 45, 540, 789, 1074, 1137
SatI GCNGC 5 cut(s) 468, 639, 1100, 1584, 1677
Sau3AI GATC 7 cut(s) 409, 967, 981, 1292, 1330, 1503, 1705
Sau96I GGNCC 3 cut(s) 86, 261, 307
ScaI AGTACT 1 cut(s) 493
SchI GAGTC 1 cut(s) 1758
ScrFI CCNGG 1 cut(s) 1632
SduI GDGCHC 3 cut(s) 631, 736, 1255
SfaNI GCATC 7 cut(s) 481, 752, 763, 856, 1150, 1157, 1599
SfcI CTRYAG 1 cut(s) 1419
SinI GGWCC 2 cut(s) 261, 307
SmiMI CAYNNNNRTG 4 cut(s) 771, 1158, 1409, 1736
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 6 cut(s) 60, 392, 403, 424, 597, 1468
SseBI AGGCCT 2 cut(s) 338, 1263
SsiI CCGC 1 cut(s) 670
SspMI CTAG 4 cut(s) 930, 1478, 1511, 1781
StuI AGGCCT 2 cut(s) 338, 1263
StyD4I CCNGG 1 cut(s) 1630
StyI CCWWGG 2 cut(s) 82, 985
TaaI ACNGT 3 cut(s) 388, 619, 1526
TaiI ACGT 3 cut(s) 213, 957, 1539
TaqI TCGA 2 cut(s) 606, 778
TasI AATT 6 cut(s) 60, 392, 403, 424, 597, 1468
TatI WGTACW 5 cut(s) 187, 491, 747, 934, 1009
TfiI GAWTC 2 cut(s) 1459, 1514
Tru1I TTAA 5 cut(s) 45, 540, 789, 1074, 1137
Tru9I TTAA 5 cut(s) 45, 540, 789, 1074, 1137
TscAI CASTG 2 cut(s) 876, 1531
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 5 cut(s) 467, 638, 1099, 1583, 1676
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 7 cut(s) 581, 668, 767, 1219, 1233, 1313, 1619
TspGWI ACGGA 1 cut(s) 1204
TspRI CASTG 2 cut(s) 876, 1531
VpaK11BI GGWCC 2 cut(s) 261, 307
VspI ATTAAT 1 cut(s) 1137
XapI RAATTY 5 cut(s) 60, 392, 403, 424, 1468
XceI RCATGY 3 cut(s) 1024, 1408, 1735
XcmI CCANNNNNNNNNTGG 1 cut(s) 1638
XspI CTAG 4 cut(s) 930, 1478, 1511, 1781
ZrmI AGTACT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.