AT1G50450

Saccharopine dehydrogenase NADP binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011377
Physical Location & Seq
Reverse (-)
18687785 .. 18690503
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50450.1

Sequence Viewer

Length: 1287 bp
ATGACTCGAGCTCTGCTGCTGCAACCGTACAGAGCGACGGTGCGAGCAGCTTCATCTAGGGAAACGCAATACGACGGCGTTCCGGAGGTTAAGTTCTCGGATCCTAGCCGGAACTATCGGGTCTTGGTGTTGGGTGGAACGGGTCGGGTCGGTGGGTCTACTGCTACTGCTCTTTCTAAGCTATGTCCTGAGCTAAAAATCGTCGTCGGTGGACGCAACAGAGAGAAAGGTGAAGCAATGGTGGCTAAGCTGGGAGAGAATTCTGAATTTTCTCAAGTAGATATCAATGATGCTAAGATGCTTGAGACAAGTTTAAGAGACGTTGATCTTGTAGTCCATGCCGCTGGACCATTCCAACAAGCACCTCGATGCACTGTTCTTGAAGCTGCCATAAAGACCAAGACTGCATATCTCGATGTTTGTGATGACACAAGCTATGCTTTCCGTGCAAAATCTCTTGAAGCTGAGGCAATTGCTGCGAATATTCCGGCGTTAACAACAGCTGGAATATATCCAGGAGTTAGCAATGTGATGGCGGCTGAAATGGTCGCTGCAGCAAGAAGCGAAGATAAAGGAAAACCAGAGAAATTGAGGTTCTCCTATTACACAGCAGGCACTGGCGGTGCAGGTCCTACAATATTAGCAACGAGTTTTCTACTTCTTGGAGAGGAAGTTACTGCATATAAACAAGGAGAGAAGGTAAAACTAAGACCCTACAGTGGAATGATAACCGTGGATTTCGGAAAAGGCATCCGGAAAAGAGATGTTTATCTTTTGAATTTGCCAGAGGTGAGATCAACTCATGAAGTTCTAGGAGTGCCAACTGTCGTTGCTCGATTCGGCACAGCTCCTTTCTTCTGGAACTGGGGAATGGAGATAATGACAAAACTCTTACCATCGGAGGTCCTTAGAGACAGAACCAAAGTCCAACAAATGGTAGAGCTTTTTGACCCTGTCGTTAGAGCCATGGATGGCTTTGCTGGAGAGCGTGTCTCAATGAGGGTTGATTTGGAGTGCTCGGATGGACGGACCACAGTTGGGTTATTTAGTCATAAAAAACTATCAGTATCCGTTGGAGTTTCGACCGCAGCTTTTGTTGCAGCAATGCTTGAAGGAAGCACACAACCAGGGGTTTGGTTTCCAGAAGAGCCTCAAGGGATCGCAGTGGAAGCAAGGGAAGTTCTTCTCAAACGTGCATCACAAGGAACATTCAATTTCATACTGAACAAGCCACCCTGGATGGTTGAGACCGAGCCGAAGGAGGTGGTTTTAGGGATATACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sacchrp_dh_NADP

Protein Analysis

428

Amino Acids

46.56

Weight (kDa)

8.19

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 41 - 160 8.6e-18 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 617
AccB7I CCANNNNNTGG 1 cut(s) 934
AccI GTMKAC 1 cut(s) 158
AccIII TCCGGA 2 cut(s) 82, 753
AciI CCGC 4 cut(s) 342, 536, 621, 1086
AclWI GGATC 3 cut(s) 95, 108, 1166
AcsI RAATTY 3 cut(s) 259, 266, 778
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 3 cut(s) 719, 934, 1038
AgsI TTSAA 5 cut(s) 383, 461, 778, 1112, 1213
AjnI CCWGG 3 cut(s) 514, 1126, 1235
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
Alw21I GWGCWC 2 cut(s) 13, 1019
Alw26I GTCTC 5 cut(s) 299, 312, 906, 997, 1241
AlwI GGATC 3 cut(s) 95, 108, 1166
AlwNI CAGNNNCTG 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 6
Aor13HI TCCGGA 2 cut(s) 82, 753
ApeKI GCWGC 9 cut(s) 16, 19, 47, 386, 476, 551, 554, 1088, 1100
ApoI RAATTY 3 cut(s) 259, 266, 778
Asp700I GAANNNNTTC 1 cut(s) 1182
AspS9I GGNCC 4 cut(s) 347, 629, 904, 1029
AsuHPI GGTGA 2 cut(s) 242, 802
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 4 cut(s) 347, 629, 904, 1029
BamHI GGATCC 1 cut(s) 100
BanII GRGCYC 1 cut(s) 13
Bbv12I GWGCWC 2 cut(s) 13, 1019
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 9 cut(s) 3, 6, 59, 373, 463, 538, 566, 1100, 1112
BccI CCATC 5 cut(s) 526, 904, 965, 1016, 1234
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 3 cut(s) 516, 1128, 1237
BciVI GTATCC 1 cut(s) 1078
BcoDI GTCTC 5 cut(s) 299, 312, 906, 997, 1241
BfaI CTAG 3 cut(s) 57, 105, 812
BfmI CTRYAG 2 cut(s) 552, 715
BfuAI ACCTGC 1 cut(s) 617
BfuI GTATCC 1 cut(s) 1078
BlpI GCTNAGC 1 cut(s) 246
Bme1390I CCNGG 3 cut(s) 516, 1128, 1237
Bme18I GGWCC 4 cut(s) 347, 629, 904, 1029
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 4 cut(s) 347, 629, 904, 1029
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 3 cut(s) 516, 1128, 1237
BmrI ACTGGG 1 cut(s) 874
BmsI GCATC 5 cut(s) 280, 288, 359, 759, 1205
BmuI ACTGGG 1 cut(s) 874
BplI GAGNNNNNCTC 4 cut(s) 784, 816, 977, 1009
BpmI CTGGAG 1 cut(s) 1002
Bpu10I CCTNAGC 2 cut(s) 189, 465
Bpu1102I GCTNAGC 1 cut(s) 246
BpuEI CTTGAG 3 cut(s) 258, 323, 1137
BsaAI YACGTR 1 cut(s) 1282
BsaBI GATNNNNATC 1 cut(s) 768
BsaI GGTCTC 1 cut(s) 1241
BsaJI CCNNGG 4 cut(s) 732, 966, 1127, 1235
BsaWI WCCGGW 2 cut(s) 82, 753
BsaXI ACNNNNNCTCC 8 cut(s) 77, 107, 657, 684, 687, 714, 975, 1005
Bsc4I CCNNNNNNNGG 3 cut(s) 719, 934, 1038
Bse1I ACTGG 2 cut(s) 622, 869
Bse3DI GCAATG 3 cut(s) 243, 532, 1110
Bse8I GATNNNNATC 1 cut(s) 768
BseAI TCCGGA 2 cut(s) 82, 753
BseBI CCWGG 3 cut(s) 516, 1128, 1237
BseDI CCNNGG 4 cut(s) 732, 966, 1127, 1235
BseGI GGATG 4 cut(s) 750, 976, 1027, 1245
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 3 cut(s) 719, 934, 1038
BseMI GCAATG 3 cut(s) 243, 532, 1110
BseMII CTCAG 2 cut(s) 180, 456
BseNI ACTGG 2 cut(s) 622, 869
BseXI GCAGC 9 cut(s) 3, 6, 59, 373, 463, 538, 566, 1100, 1112
BseYI CCCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 645
Bsh1285I CGRYCG 1 cut(s) 1086
BsiEI CGRYCG 1 cut(s) 1086
BsiHKAI GWGCWC 2 cut(s) 13, 1019
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 4 cut(s) 83, 109, 488, 754
BslI CCNNNNNNNGG 3 cut(s) 719, 934, 1038
BsmAI GTCTC 5 cut(s) 299, 312, 906, 997, 1241
BsmBI CGTCTC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 1241
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 2 cut(s) 13, 1019
Bsp13I TCCGGA 2 cut(s) 82, 753
Bsp143I GATC 4 cut(s) 100, 325, 794, 1158
Bsp1720I GCTNAGC 1 cut(s) 246
Bsp19I CCATGG 1 cut(s) 966
BspACI CCGC 4 cut(s) 342, 536, 621, 1086
BspCNI CTCAG 2 cut(s) 181, 457
BspEI TCCGGA 2 cut(s) 82, 753
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 1 cut(s) 102
BspMAI CTGCAG 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 3 cut(s) 95, 108, 1166
BspQI GCTCTTC 1 cut(s) 1140
BspTNI GGTCTC 1 cut(s) 1241
BsrDI GCAATG 3 cut(s) 243, 532, 1110
BsrI ACTGG 2 cut(s) 622, 869
BssECI CCNNGG 4 cut(s) 732, 966, 1127, 1235
BssMI GATC 4 cut(s) 100, 325, 794, 1158
BssT1I CCWWGG 1 cut(s) 966
Bst2UI CCWGG 3 cut(s) 516, 1128, 1237
Bst4CI ACNGT 7 cut(s) 27, 40, 376, 719, 733, 826, 1036
Bst6I CTCTTC 1 cut(s) 1140
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 1282
BstC8I GCNNGC 2 cut(s) 45, 613
BstDEI CTNAG 7 cut(s) 177, 189, 246, 294, 465, 707, 908
BstDSI CCRYGG 2 cut(s) 732, 966
BstF5I GGATG 4 cut(s) 750, 976, 1027, 1245
BstKTI GATC 4 cut(s) 103, 328, 797, 1161
BstMAI GTCTC 5 cut(s) 299, 312, 906, 997, 1241
BstMBI GATC 4 cut(s) 100, 325, 794, 1158
BstMCI CGRYCG 1 cut(s) 1086
BstMWI GCNNNNNNNGC 5 cut(s) 242, 446, 476, 1097, 1169
BstNI CCWGG 3 cut(s) 516, 1128, 1237
BstSCI CCNGG 3 cut(s) 514, 1126, 1235
BstSFI CTRYAG 2 cut(s) 552, 715
BstSNI TACGTA 1 cut(s) 1282
BstV1I GCAGC 9 cut(s) 3, 6, 59, 373, 463, 538, 566, 1100, 1112
BstX2I RGATCY 1 cut(s) 100
BstXI CCANNNNNNTGG 2 cut(s) 344, 1134
BstYI RGATCY 1 cut(s) 100
BsuI GTATCC 1 cut(s) 1078
BtgI CCRYGG 2 cut(s) 732, 966
BtsCI GGATG 4 cut(s) 750, 976, 1027, 1245
BtsI GCAGTG 1 cut(s) 1170
BtsIMutI CAGTG 4 cut(s) 372, 615, 724, 1170
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 2 cut(s) 45, 613
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 802
Cfr13I GGNCC 4 cut(s) 347, 629, 904, 1029
CseI GACGC 1 cut(s) 222
Csp6I GTAC 1 cut(s) 28
CviAII CATG 3 cut(s) 338, 803, 967
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 7 cut(s) 177, 189, 246, 294, 465, 707, 908
DpnI GATC 4 cut(s) 102, 327, 796, 1160
DpnII GATC 4 cut(s) 100, 325, 794, 1158
Eam1104I CTCTTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1140
Ecl136II GAGCTC 1 cut(s) 11
Eco105I TACGTA 1 cut(s) 1282
Eco130I CCWWGG 1 cut(s) 966
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 1241
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 4 cut(s) 347, 629, 904, 1029
Eco53kI GAGCTC 1 cut(s) 11
Eco88I CYCGRG 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 11
EcoO109I RGGNCCY 2 cut(s) 629, 904
EcoRI GAATTC 1 cut(s) 259
EcoRII CCWGG 3 cut(s) 514, 1126, 1235
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 966
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 966
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 3 cut(s) 341, 806, 970
FalI AAGNNNNNCTT 2 cut(s) 424, 456
FatI CATG 3 cut(s) 337, 802, 966
FblI GTMKAC 1 cut(s) 158
FokI GGATG 4 cut(s) 737, 983, 1034, 1252
FriOI GRGCYC 1 cut(s) 13
FspBI CTAG 3 cut(s) 57, 105, 812
GsaI CCCAGC 1 cut(s) 254
GsuI CTGGAG 1 cut(s) 1002
HapII CCGG 4 cut(s) 83, 109, 488, 754
HgaI GACGC 1 cut(s) 222
Hin1II CATG 3 cut(s) 341, 806, 970
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HinfI GANTC 2 cut(s) 4, 837
HpaI GTTAAC 1 cut(s) 495
HpaII CCGG 4 cut(s) 83, 109, 488, 754
HphI GGTGA 2 cut(s) 242, 802
Hpy166II GTNNAC 3 cut(s) 159, 212, 495
Hpy188I TCNGA 5 cut(s) 100, 265, 743, 901, 1021
Hpy188III TCNNGA 9 cut(s) 83, 188, 380, 413, 458, 754, 803, 859, 1142
Hpy8I GTNNAC 3 cut(s) 159, 212, 495
Hpy99I CGWCG 4 cut(s) 40, 77, 206, 209
HpyAV CCTTC 3 cut(s) 691, 1106, 1252
HpyCH4III ACNGT 7 cut(s) 27, 40, 376, 719, 733, 826, 1036
HpyCH4IV ACGT 3 cut(s) 321, 1192, 1281
HpyCH4V TGCA 9 cut(s) 22, 372, 407, 449, 554, 626, 680, 1100, 1196
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 446, 476, 1097, 1169
HpyF3I CTNAG 7 cut(s) 177, 189, 246, 294, 465, 707, 908
HpySE526I ACGT 3 cut(s) 321, 1192, 1281
Hsp92II CATG 3 cut(s) 341, 806, 970
Kpn2I TCCGGA 2 cut(s) 82, 753
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 4 cut(s) 100, 325, 794, 1158
LguI GCTCTTC 1 cut(s) 1140
LmnI GCTCC 1 cut(s) 853
Lsp1109I GCAGC 9 cut(s) 3, 6, 59, 373, 463, 538, 566, 1100, 1112
LweI GCATC 5 cut(s) 280, 288, 359, 759, 1205
MaeI CTAG 3 cut(s) 57, 105, 812
MaeII ACGT 3 cut(s) 321, 1192, 1281
MaeIII GTNAC 1 cut(s) 673
MalI GATC 4 cut(s) 102, 327, 796, 1160
MboI GATC 4 cut(s) 100, 325, 794, 1158
MboII GAAGA 4 cut(s) 578, 847, 1157, 1175
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 13, 1019
MluCI AATT 6 cut(s) 259, 266, 471, 587, 778, 1213
MmeI TCCRAC 3 cut(s) 379, 952, 1054
MroI TCCGGA 2 cut(s) 82, 753
MroXI GAANNNNTTC 1 cut(s) 1182
MseI TTAA 3 cut(s) 90, 314, 494
MslI CAYNNNNRTG 1 cut(s) 367
MspA1I CMGCKG 2 cut(s) 344, 503
MspI CCGG 4 cut(s) 83, 109, 488, 754
MspR9I CCNGG 3 cut(s) 516, 1128, 1237
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 3 cut(s) 516, 1128, 1237
MwoI GCNNNNNNNGC 5 cut(s) 242, 446, 476, 1097, 1169
NcoI CCATGG 1 cut(s) 966
NdeII GATC 4 cut(s) 100, 325, 794, 1158
NlaIII CATG 3 cut(s) 341, 806, 970
NlaIV GGNNCC 1 cut(s) 102
PaeR7I CTCGAG 1 cut(s) 6
PagI TCATGA 1 cut(s) 802
PciSI GCTCTTC 1 cut(s) 1140
PdmI GAANNNNTTC 1 cut(s) 1182
PfeI GAWTC 1 cut(s) 837
PflFI GACNNNGTC 1 cut(s) 953
PflMI CCANNNNNTGG 1 cut(s) 934
PfoI TCCNGGA 1 cut(s) 514
Ppu21I YACGTR 1 cut(s) 1282
PpuMI RGGWCCY 2 cut(s) 629, 904
Psp124BI GAGCTC 1 cut(s) 13
Psp5II RGGWCCY 2 cut(s) 629, 904
Psp6I CCWGG 3 cut(s) 514, 1126, 1235
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 3 cut(s) 514, 1126, 1235
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 4 cut(s) 347, 629, 904, 1029
PspPPI RGGWCCY 2 cut(s) 629, 904
PspXI VCTCGAGB 1 cut(s) 6
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 1 cut(s) 100
PsyI GACNNNGTC 1 cut(s) 953
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 1 cut(s) 367
SacI GAGCTC 1 cut(s) 13
SapI GCTCTTC 1 cut(s) 1140
SaqAI TTAA 3 cut(s) 90, 314, 494
Sau3AI GATC 4 cut(s) 100, 325, 794, 1158
Sau96I GGNCC 4 cut(s) 347, 629, 904, 1029
ScrFI CCNGG 3 cut(s) 516, 1128, 1237
SduI GDGCHC 2 cut(s) 13, 1019
SfaNI GCATC 5 cut(s) 280, 288, 359, 759, 1205
SfcI CTRYAG 2 cut(s) 552, 715
Sfr274I CTCGAG 1 cut(s) 6
SinI GGWCC 4 cut(s) 347, 629, 904, 1029
SlaI CTCGAG 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 367
SmlI CTYRAG 4 cut(s) 6, 273, 302, 1152
SmoI CTYRAG 4 cut(s) 6, 273, 302, 1152
SnaBI TACGTA 1 cut(s) 1282
Sse9I AATT 6 cut(s) 259, 266, 471, 587, 778, 1213
SsiI CCGC 4 cut(s) 342, 536, 621, 1086
SspI AATATT 2 cut(s) 484, 639
SspMI CTAG 3 cut(s) 57, 105, 812
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 3 cut(s) 514, 1126, 1235
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 7 cut(s) 27, 40, 376, 719, 733, 826, 1036
TaiI ACGT 3 cut(s) 324, 1195, 1284
TaqI TCGA 5 cut(s) 7, 367, 414, 835, 1082
TaqII GACCGA 1 cut(s) 1265
TasI AATT 6 cut(s) 259, 266, 471, 587, 778, 1213
TauI GCSGC 2 cut(s) 344, 539
TfiI GAWTC 1 cut(s) 837
Tru1I TTAA 3 cut(s) 90, 314, 494
Tru9I TTAA 3 cut(s) 90, 314, 494
TscAI CASTG 4 cut(s) 379, 622, 724, 1170
TseI GCWGC 9 cut(s) 16, 19, 47, 386, 476, 551, 554, 1088, 1100
TspDTI ATGAA 3 cut(s) 42, 819, 1207
TspGWI ACGGA 3 cut(s) 434, 1042, 1060
TspRI CASTG 4 cut(s) 379, 622, 724, 1170
Tth111I GACNNNGTC 1 cut(s) 953
Van91I CCANNNNNTGG 1 cut(s) 934
VpaK11BI GGWCC 4 cut(s) 347, 629, 904, 1029
XapI RAATTY 3 cut(s) 259, 266, 778
XhoI CTCGAG 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 158
XmnI GAANNNNTTC 1 cut(s) 1182
XspI CTAG 3 cut(s) 57, 105, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.