Rh5AG339700

Saccharopine dehydrogenase NADP binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011377
Physical Location & Seq
Forward (+)
55117322 .. 55123022
5701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG339700.1

Sequence Viewer

Length: 1041 bp
ATGCTACATGGCTGCAATTGGGTTTCATACAGGGAGAAAGGCGCTGAAATGCTGACTACTCTTGGGGCGAACTCGGAGTTCTATGAAGTCGATATTAACAATGAAAAATCATTGGAAGCAGCTCTGGAAGATGCGGATCTTGTAGTTCATGCTGCTGGCCCATTCCAACAGGCAGAGAAGTGCACTGTATTGGAAGCTGCCTTAAAGACCAAGACAGCCTATGTTGATGTTTGTGATGATACAGCCTATGCATTCCGTGCAAAATCTTTCCACGATAGAGCAGTTGCAGCTAATGTTCCAGCCATAACAACTGGTGGAATATATCCAGGAGTGAGCAATGTGATGGCTGCAGAACTAGTTCGTGTCGCAAGAAGTGAAAACAAAGGAAAACCAGAAAGGCTAAGGTTCTTCTACTACACAGCAGGTACTGGTGGGGCTGGTCCAACCATCTTAGCTACTAGTTTTTTGCTTCTTGGAGAGGAGGTTGTTGCATTTAATAAAGGAGAAAAGATCAAACTAAGGCCATATAGTGGAATGCTGAAGATTGACTTTGGAAAAGGCATTGGAAAGAAAGATGTTTATCTATTGAATTTGCCAGAGGTAAGAAGTACTCACGAGGTCCTAGGAGTACCTACTGTCAGTGCCCGGTTTGGAACTGCCCCCTTCTTTTGGAATTGGGCAATGGAAGCCATGACTATTCTTCCTGCAGAGTTGTTGAGAGACAGAAGCAAAGTCCAACAGATGGTTCAAATGTTTGACCCTGTAGTCAGAGCAGTTGATGAGATTGCTGGAGAGCGTGTATCAATGCGGGTTGATTTGGACTGTTCTGGCGGCCGCAATACAGTAAGTGTATTCAGTCACAAGAGGCTTTCTGTATCAGTTGGAACTGCAACATCTGCATTTGTTCTGGCAGTTCTTGAGGGAAGCACAAAGCCTGGAGTTTGGTTTCCAGAAGAGGCTGAAGGGATTGCAATTGAGGCAAGGGAAGTCCTTCTCAAACGTGCTGCACAAGGCACAATAAATTTTGTTATGAACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sacchrp_dh_NADP

Protein Analysis

346

Amino Acids

37.71

Weight (kDa)

6.27

Isoelectric Point (pI)

28.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 13 - 99 6.8e-11 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 413
AccB7I CCANNNNNTGG 2 cut(s) 530, 742
AciI CCGC 4 cut(s) 134, 808, 831, 835
AclWI GGATC 1 cut(s) 144
AcoI YGGCCR 1 cut(s) 832
AcsI RAATTY 2 cut(s) 589, 1021
AcuI CTGAAG 2 cut(s) 560, 981
AfaI GTAC 3 cut(s) 427, 610, 630
AfiI CCNNNNNNNGG 3 cut(s) 530, 669, 742
AgsI TTSAA 2 cut(s) 589, 749
AhlI ACTAGT 2 cut(s) 355, 458
AjnI CCWGG 2 cut(s) 325, 934
AjuI GAANNNNNNNTTGG 2 cut(s) 729, 761
AluBI AGCT 4 cut(s) 122, 197, 290, 455
AluI AGCT 4 cut(s) 122, 197, 290, 455
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 714
Alw44I GTGCAC 1 cut(s) 181
AlwI GGATC 1 cut(s) 144
AlwNI CAGNNNCTG 1 cut(s) 428
AoxI GGCC 3 cut(s) 157, 521, 832
ApaLI GTGCAC 1 cut(s) 181
ApeKI GCWGC 7 cut(s) 12, 119, 152, 197, 287, 347, 1004
ApoI RAATTY 2 cut(s) 589, 1021
Asp700I GAANNNNTTC 2 cut(s) 357, 990
AspA2I CCTAGG 1 cut(s) 622
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 3 cut(s) 158, 440, 619
AsuC2I CCSGG 1 cut(s) 646
AvaII GGWCC 2 cut(s) 440, 619
AvrII CCTAGG 1 cut(s) 622
BaeGI GKGCMC 2 cut(s) 185, 646
BauI CACGAG 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 185
BbvI GCAGC 6 cut(s) 131, 139, 184, 299, 334, 991
BccI CCATC 3 cut(s) 337, 455, 736
BciT130I CCWGG 2 cut(s) 327, 936
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 1 cut(s) 714
BcuI ACTAGT 2 cut(s) 355, 458
BfaI CTAG 3 cut(s) 356, 459, 623
BfmI CTRYAG 3 cut(s) 348, 705, 762
BfoI RGCGCY 1 cut(s) 45
BfuAI ACCTGC 1 cut(s) 413
BisI GCNGC 9 cut(s) 13, 120, 153, 198, 288, 348, 832, 835, 1005
BlnI CCTAGG 1 cut(s) 622
BlsI GCNGC 9 cut(s) 14, 121, 154, 199, 289, 349, 833, 836, 1006
BmcAI AGTACT 1 cut(s) 610
Bme1390I CCNGG 3 cut(s) 327, 646, 936
Bme18I GGWCC 2 cut(s) 440, 619
BmgT120I GGNCC 3 cut(s) 158, 440, 619
BmrFI CCNGG 3 cut(s) 327, 646, 936
BmsI GCATC 1 cut(s) 121
BpmI CTGGAG 2 cut(s) 810, 957
Bpu10I CCTNAGC 1 cut(s) 401
BpuEI CTTGAG 1 cut(s) 938
BpuMI CCSGG 1 cut(s) 646
BsaBI GATNNNNATC 2 cut(s) 135, 579
BsaJI CCNNGG 1 cut(s) 622
BsaXI ACNNNNNCTCC 4 cut(s) 468, 498, 783, 813
Bsc4I CCNNNNNNNGG 3 cut(s) 530, 669, 742
Bse1I ACTGG 2 cut(s) 316, 433
Bse3DI GCAATG 2 cut(s) 343, 687
Bse8I GATNNNNATC 2 cut(s) 135, 579
BseBI CCWGG 2 cut(s) 327, 936
BseDI CCNNGG 1 cut(s) 622
BseJI GATNNNNATC 2 cut(s) 135, 579
BseLI CCNNNNNNNGG 3 cut(s) 530, 669, 742
BseMI GCAATG 2 cut(s) 343, 687
BseNI ACTGG 2 cut(s) 316, 433
BseRI GAGGAG 1 cut(s) 494
BseSI GKGCMC 2 cut(s) 185, 646
BseX3I CGGCCG 1 cut(s) 832
BseXI GCAGC 6 cut(s) 131, 139, 184, 299, 334, 991
BsgI GTGCAG 1 cut(s) 990
Bsh1285I CGRYCG 1 cut(s) 835
BshFI GGCC 3 cut(s) 159, 523, 834
BsiEI CGRYCG 1 cut(s) 835
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 1 cut(s) 646
BslI CCNNNNNNNGG 3 cut(s) 530, 669, 742
BsmAI GTCTC 1 cut(s) 714
BsmI GAATGC 2 cut(s) 251, 540
BsnI GGCC 3 cut(s) 159, 523, 834
Bsp1286I GDGCHC 2 cut(s) 185, 646
Bsp143I GATC 2 cut(s) 136, 510
BspACI CCGC 4 cut(s) 134, 808, 831, 835
BspANI GGCC 3 cut(s) 159, 523, 834
BspMAI CTGCAG 2 cut(s) 352, 709
BspMI ACCTGC 1 cut(s) 413
BspPI GGATC 1 cut(s) 144
BsrDI GCAATG 2 cut(s) 343, 687
BsrI ACTGG 2 cut(s) 316, 433
BssECI CCNNGG 1 cut(s) 622
BssMI GATC 2 cut(s) 136, 510
BssSI CACGAG 1 cut(s) 614
BssT1I CCWWGG 1 cut(s) 622
Bst2BI CACGAG 1 cut(s) 614
Bst2UI CCWGG 2 cut(s) 327, 936
Bst4CI ACNGT 4 cut(s) 187, 637, 824, 844
Bst6I CTCTTC 1 cut(s) 948
BstAPI GCANNNNNTGC 2 cut(s) 257, 896
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 3 cut(s) 401, 451, 518
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 2 cut(s) 139, 513
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 2 cut(s) 136, 510
BstMCI CGRYCG 1 cut(s) 835
BstMWI GCNNNNNNNGC 5 cut(s) 257, 287, 686, 896, 977
BstNI CCWGG 2 cut(s) 327, 936
BstSCI CCNGG 3 cut(s) 325, 644, 934
BstSFI CTRYAG 3 cut(s) 348, 705, 762
BstSLI GKGCMC 2 cut(s) 185, 646
BstV1I GCAGC 6 cut(s) 131, 139, 184, 299, 334, 991
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BstZI CGGCCG 1 cut(s) 832
BsuRI GGCC 3 cut(s) 159, 523, 834
BtsIMutI CAGTG 2 cut(s) 183, 646
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 1 cut(s) 157
CaiI CAGNNNCTG 1 cut(s) 428
CciNI GCGGCCGC 1 cut(s) 832
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 3 cut(s) 158, 440, 619
Csp6I GTAC 3 cut(s) 426, 609, 629
CviAII CATG 3 cut(s) 8, 149, 691
CviQI GTAC 3 cut(s) 426, 609, 629
DdeI CTNAG 3 cut(s) 401, 451, 518
DpnI GATC 2 cut(s) 138, 512
DpnII GATC 2 cut(s) 136, 510
DrdI GACNNNNNNGTC 1 cut(s) 764
DseDI GACNNNNNNGTC 1 cut(s) 764
EaeI YGGCCR 1 cut(s) 832
EagI CGGCCG 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 948
EarI CTCTTC 1 cut(s) 948
EclXI CGGCCG 1 cut(s) 832
Eco130I CCWWGG 1 cut(s) 622
Eco47I GGWCC 2 cut(s) 440, 619
Eco52I CGGCCG 1 cut(s) 832
Eco57I CTGAAG 2 cut(s) 560, 981
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 325, 934
EcoT14I CCWWGG 1 cut(s) 622
EcoT22I ATGCAT 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 622
FaeI CATG 3 cut(s) 11, 152, 694
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FatI CATG 3 cut(s) 7, 148, 690
FauI CCCGC 1 cut(s) 801
Fnu4HI GCNGC 9 cut(s) 13, 120, 153, 198, 288, 348, 832, 835, 1005
Fsp4HI GCNGC 9 cut(s) 13, 120, 153, 198, 288, 348, 832, 835, 1005
FspBI CTAG 3 cut(s) 356, 459, 623
GlaI GCGC 1 cut(s) 43
GluI GCNGC 9 cut(s) 13, 120, 153, 198, 288, 348, 832, 835, 1005
GsuI CTGGAG 2 cut(s) 810, 957
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 3 cut(s) 159, 523, 834
HapII CCGG 1 cut(s) 646
HhaI GCGC 1 cut(s) 44
Hin1II CATG 3 cut(s) 11, 152, 694
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HpaII CCGG 1 cut(s) 646
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 2 cut(s) 76, 770
Hpy188III TCNNGA 4 cut(s) 125, 614, 917, 950
Hpy8I GTNNAC 1 cut(s) 183
HpyAV CCTTC 3 cut(s) 673, 956, 1001
HpyCH4III ACNGT 4 cut(s) 187, 637, 824, 844
HpyCH4IV ACGT 1 cut(s) 1000
HpyF10VI GCNNNNNNNGC 5 cut(s) 257, 287, 686, 896, 977
HpyF3I CTNAG 3 cut(s) 401, 451, 518
HpySE526I ACGT 1 cut(s) 1000
Hsp92II CATG 3 cut(s) 11, 152, 694
HspAI GCGC 1 cut(s) 42
Kzo9I GATC 2 cut(s) 136, 510
Lsp1109I GCAGC 6 cut(s) 131, 139, 184, 299, 334, 991
LweI GCATC 1 cut(s) 121
MaeI CTAG 3 cut(s) 356, 459, 623
MaeII ACGT 1 cut(s) 1000
MaeIII GTNAC 1 cut(s) 857
MalI GATC 2 cut(s) 138, 512
MboI GATC 2 cut(s) 136, 510
MboII GAAGA 5 cut(s) 140, 400, 553, 692, 965
MfeI CAATTG 2 cut(s) 16, 972
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 185, 646
MluCI AATT 5 cut(s) 16, 589, 673, 972, 1021
MmeI TCCRAC 4 cut(s) 190, 467, 760, 862
MnlI CCTC 8 cut(s) 472, 475, 592, 610, 858, 913, 949, 970
Mph1103I ATGCAT 1 cut(s) 253
MroXI GAANNNNTTC 2 cut(s) 357, 990
MseI TTAA 3 cut(s) 96, 203, 495
MspI CCGG 1 cut(s) 646
MspR9I CCNGG 3 cut(s) 327, 646, 936
MunI CAATTG 2 cut(s) 16, 972
Mva1269I GAATGC 2 cut(s) 251, 540
MvaI CCWGG 2 cut(s) 327, 936
MwoI GCNNNNNNNGC 5 cut(s) 257, 287, 686, 896, 977
NciI CCSGG 1 cut(s) 646
NdeII GATC 2 cut(s) 136, 510
NlaIII CATG 3 cut(s) 11, 152, 694
NmuCI GTSAC 1 cut(s) 857
NotI GCGGCCGC 1 cut(s) 832
NsiI ATGCAT 1 cut(s) 253
PctI GAATGC 2 cut(s) 251, 540
PdmI GAANNNNTTC 2 cut(s) 357, 990
PflMI CCANNNNNTGG 2 cut(s) 530, 742
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 9 cut(s) 14, 121, 154, 199, 289, 349, 833, 836, 1006
PpuMI RGGWCCY 1 cut(s) 619
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 2 cut(s) 325, 934
PspGI CCWGG 2 cut(s) 325, 934
PspPI GGNCC 3 cut(s) 158, 440, 619
PspPPI RGGWCCY 1 cut(s) 619
PstI CTGCAG 2 cut(s) 352, 709
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 1 cut(s) 136
RsaI GTAC 3 cut(s) 427, 610, 630
RsaNI GTAC 3 cut(s) 426, 609, 629
SaqAI TTAA 3 cut(s) 96, 203, 495
SatI GCNGC 9 cut(s) 13, 120, 153, 198, 288, 348, 832, 835, 1005
Sau3AI GATC 2 cut(s) 136, 510
Sau96I GGNCC 3 cut(s) 158, 440, 619
ScaI AGTACT 1 cut(s) 610
ScrFI CCNGG 3 cut(s) 327, 646, 936
SduI GDGCHC 2 cut(s) 185, 646
SfaNI GCATC 1 cut(s) 121
SfcI CTRYAG 3 cut(s) 348, 705, 762
SinI GGWCC 2 cut(s) 440, 619
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SpeI ACTAGT 2 cut(s) 355, 458
Sse9I AATT 5 cut(s) 16, 589, 673, 972, 1021
SsiI CCGC 4 cut(s) 134, 808, 831, 835
SspMI CTAG 3 cut(s) 356, 459, 623
StyD4I CCNGG 3 cut(s) 325, 644, 934
StyI CCWWGG 1 cut(s) 622
TaaI ACNGT 4 cut(s) 187, 637, 824, 844
TaiI ACGT 1 cut(s) 1003
TaqI TCGA 1 cut(s) 90
TasI AATT 5 cut(s) 16, 589, 673, 972, 1021
TatI WGTACW 1 cut(s) 608
TauI GCSGC 2 cut(s) 834, 837
Tru1I TTAA 3 cut(s) 96, 203, 495
Tru9I TTAA 3 cut(s) 96, 203, 495
TscAI CASTG 2 cut(s) 190, 646
TseFI GTSAC 1 cut(s) 857
TseI GCWGC 7 cut(s) 12, 119, 152, 197, 287, 347, 1004
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 4 cut(s) 15, 99, 117, 137
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 2 cut(s) 190, 646
Van91I CCANNNNNTGG 2 cut(s) 530, 742
VneI GTGCAC 1 cut(s) 181
VpaK11BI GGWCC 2 cut(s) 440, 619
XapI RAATTY 2 cut(s) 589, 1021
XmaJI CCTAGG 1 cut(s) 622
XmnI GAANNNNTTC 2 cut(s) 357, 990
XspI CTAG 3 cut(s) 356, 459, 623
ZrmI AGTACT 1 cut(s) 610
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.