FvH4_3g28350

Saccharopine dehydrogenase NADP binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011377
Physical Location & Seq
Forward (+)
21319353 .. 21325179
5827 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28350.t4

Sequence Viewer

Length: 1308 bp
ATGGCGGGAGGAGTTTCACTCTCATCATCACTGCATTTGAAGCTCAGAAGAAGAAGCTCTACAATCGTTAGTGTGGCCAGTCCTGCCGATACCCAAACTAGTTCTCGGGTCCAGCTCCCACAGAAGACAAGGAATTCGAGGGTGTTGGTGCTCGGTGGAACGGGTCGGGTCGGAGGGTCCACTGCTATTGCCCTCTCCAACCTCTGCCCCGACCTCCAAATTATCGTCGCTGGCCGAAACAGGGAAAAAGGTGCTGATATGCTGACTACTCTTGGGGGGAACTCGGAGTTCTGTGAAGTCGATATCAACAATGAAAAATCGTTGGAAGCTGCTCTAGAAGATGCCGATCTTGTAGTTCATGCTGCCGGCCCATTCCAACAGGCAGAGAAGTGCACTGTATTGGAAGCTGCCTTAAAGACCAAGACAGCCTATATTGATGTTTGTGATGATACAGCCTATGCATTCCGTGCGAAATCTTTTCACGATAGAGCAGTTGCAGCTAATGTTCCAGCTATAACAACTGGTGGAATATATCCAGGAGTGAGCAATGTGATGGCTGCAGAACTAGTTCGTCTCGCAAGAAGTGAAAACAAAGGAAAACCAGAAAGGCTAAGGTTCTTCTACTACACAGCAGGTACTGGTGGGGCTGGTCCAACTATCTTAGCTACTAGTTTTCTGCTTCTTGGAGAAGAGGTTGTTGCATTTAATAAAGGAGAAAAGATCAAACTAAGGCCATATAGTGGAATGCTGAAGATTGACTTTGGGAAAGGCATTGGAAAGAAAGATGTTTATCTATTGAATTTGCCAGAGGTAAGAAGTACTCACGAGATCCTAGGAGTACCTACTGTCAGTGCCCGGTTCGGTACTGCCCCCTTCTTTTGGAATTGGGCAATGGAAGCCATGACTGCTCTTCTTCCTGCAGAGTTGTTGAGAGACAGAAGCAAAGTCCAACAGATGGTTAAAATGTTTGACCCAGTAGTCAGAGCAGTTGATGAGATTGCTGGAGAGCGTGTATCAATGCGGGTTGATTTGGACTGCTCTGGCGGGCGGAATACAGTGAGTGTATTCAGTCACAAGAAGCTTTCTGTATCAGTTGGAACTGCAACAACTGCATTTGTTCTGGCAGTTCTTGAGGGAAGCACAAAACCTGGGGTTTGGTTTCCGGAAGAGGCTGGAGGAATTGCAATTGAGGCAAGGGAAGTCCTGCTCAAACGTGCTGCACAAGGCACGATAAATTTTGTAACAAACAAGCCAGCATGGATGGTGGAGACAAATCCGAAAGAGTTAGGGTTGGGAATTTACGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sacchrp_dh_NADP

Protein Analysis

436

Amino Acids

46.68

Weight (kDa)

9.05

Isoelectric Point (pI)

35.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sacchrp_dh_NADP PF03435 48 - 169 6e-18 Saccharopine dehydrogenase NADP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 977
Acc36I ACCTGC 1 cut(s) 623
AccB7I CCANNNNNTGG 2 cut(s) 740, 955
AccIII TCCGGA 1 cut(s) 1162
AciI CCGC 4 cut(s) 5, 1021, 1044, 1048
AclWI GGATC 1 cut(s) 823
AcoI YGGCCR 2 cut(s) 75, 232
AcsI RAATTY 4 cut(s) 133, 799, 1234, 1296
AcuI CTGAAG 1 cut(s) 770
AfaI GTAC 4 cut(s) 637, 820, 840, 865
AfiI CCNNNNNNNGG 4 cut(s) 241, 740, 879, 955
AflIII ACRYGT 1 cut(s) 1302
AgsI TTSAA 2 cut(s) 40, 799
AhlI ACTAGT 3 cut(s) 98, 565, 668
AjnI CCWGG 2 cut(s) 535, 1147
AluBI AGCT 9 cut(s) 43, 57, 115, 329, 407, 500, 512, 665, 1081
AluI AGCT 9 cut(s) 43, 57, 115, 329, 407, 500, 512, 665, 1081
Alw21I GWGCWC 2 cut(s) 153, 395
Alw26I GTCTC 3 cut(s) 578, 927, 1262
Alw44I GTGCAC 1 cut(s) 391
AlwI GGATC 1 cut(s) 823
AlwNI CAGNNNCTG 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 105
Aor13HI TCCGGA 1 cut(s) 1162
AoxI GGCC 4 cut(s) 75, 232, 367, 731
ApaLI GTGCAC 1 cut(s) 391
ApeKI GCWGC 6 cut(s) 329, 362, 407, 497, 557, 1217
ApoI RAATTY 4 cut(s) 133, 799, 1234, 1296
ArsI GACNNNNNNTTYG 2 cut(s) 118, 150
Asp700I GAANNNNTTC 1 cut(s) 567
AspA2I CCTAGG 1 cut(s) 832
AspS9I GGNCC 4 cut(s) 109, 177, 368, 650
AsuC2I CCSGG 1 cut(s) 856
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 3 cut(s) 109, 177, 650
AvrII CCTAGG 1 cut(s) 832
BaeGI GKGCMC 2 cut(s) 395, 856
BalI TGGCCA 1 cut(s) 77
BarI GAAGNNNNNNTAC 2 cut(s) 43, 75
BauI CACGAG 1 cut(s) 824
BbsI GAAGAC 1 cut(s) 131
Bbv12I GWGCWC 2 cut(s) 153, 395
BbvI GCAGC 6 cut(s) 316, 349, 394, 509, 544, 1204
BccI CCATC 3 cut(s) 547, 949, 1255
BciT130I CCWGG 2 cut(s) 537, 1149
BcnI CCSGG 1 cut(s) 856
BcoDI GTCTC 3 cut(s) 578, 927, 1262
BcuI ACTAGT 3 cut(s) 98, 565, 668
BfaI CTAG 5 cut(s) 99, 335, 566, 669, 833
BfmI CTRYAG 2 cut(s) 558, 918
BfuAI ACCTGC 1 cut(s) 623
BisI GCNGC 6 cut(s) 330, 363, 408, 498, 558, 1218
BlnI CCTAGG 1 cut(s) 832
BlsI GCNGC 6 cut(s) 331, 364, 409, 499, 559, 1219
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 3 cut(s) 537, 856, 1149
Bme18I GGWCC 3 cut(s) 109, 177, 650
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 4 cut(s) 109, 177, 368, 650
BmiI GGNNCC 2 cut(s) 110, 178
BmrFI CCNGG 3 cut(s) 537, 856, 1149
BmrI ACTGGG 1 cut(s) 968
BmsI GCATC 1 cut(s) 331
BmuI ACTGGG 1 cut(s) 968
BpiI GAAGAC 1 cut(s) 131
BplI GAGNNNNNCTC 1 cut(s) 35
BpmI CTGGAG 2 cut(s) 1023, 1194
Bpu10I CCTNAGC 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 1151
BpuMI CCSGG 1 cut(s) 856
BsaAI YACGTR 1 cut(s) 1303
BsaBI GATNNNNATC 2 cut(s) 345, 789
BsaJI CCNNGG 2 cut(s) 832, 1148
BsaWI WCCGGW 1 cut(s) 1162
BsaXI ACNNNNNCTCC 4 cut(s) 678, 708, 996, 1026
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 740, 879, 955
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 4 cut(s) 78, 526, 643, 974
Bse3DI GCAATG 2 cut(s) 553, 897
Bse8I GATNNNNATC 2 cut(s) 345, 789
BseAI TCCGGA 1 cut(s) 1162
BseBI CCWGG 2 cut(s) 537, 1149
BseDI CCNNGG 2 cut(s) 832, 1148
BseGI GGATG 1 cut(s) 1266
BseJI GATNNNNATC 2 cut(s) 345, 789
BseLI CCNNNNNNNGG 4 cut(s) 241, 740, 879, 955
BseMI GCAATG 2 cut(s) 553, 897
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 4 cut(s) 78, 526, 643, 974
BseRI GAGGAG 1 cut(s) 24
BseSI GKGCMC 2 cut(s) 395, 856
BseXI GCAGC 6 cut(s) 316, 349, 394, 509, 544, 1204
BsgI GTGCAG 1 cut(s) 1203
BshFI GGCC 4 cut(s) 77, 234, 369, 733
BsiHKAI GWGCWC 2 cut(s) 153, 395
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 3 cut(s) 366, 856, 1163
BslI CCNNNNNNNGG 4 cut(s) 241, 740, 879, 955
BsmAI GTCTC 3 cut(s) 578, 927, 1262
BsmBI CGTCTC 1 cut(s) 578
BsmI GAATGC 2 cut(s) 461, 750
BsnI GGCC 4 cut(s) 77, 234, 369, 733
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 3 cut(s) 153, 395, 856
Bsp13I TCCGGA 1 cut(s) 1162
Bsp143I GATC 3 cut(s) 346, 720, 828
BspACI CCGC 4 cut(s) 5, 1021, 1044, 1048
BspANI GGCC 4 cut(s) 77, 234, 369, 733
BspCNI CTCAG 1 cut(s) 57
BspEI TCCGGA 1 cut(s) 1162
BspLI GGNNCC 2 cut(s) 110, 178
BspMAI CTGCAG 2 cut(s) 562, 922
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 1 cut(s) 823
BspQI GCTCTTC 1 cut(s) 915
BsrDI GCAATG 2 cut(s) 553, 897
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 4 cut(s) 78, 526, 643, 974
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 832, 1148
BssMI GATC 3 cut(s) 346, 720, 828
BssSI CACGAG 1 cut(s) 824
BssT1I CCWWGG 1 cut(s) 832
Bst2BI CACGAG 1 cut(s) 824
Bst2UI CCWGG 2 cut(s) 537, 1149
Bst4CI ACNGT 3 cut(s) 397, 847, 1057
Bst6I CTCTTC 3 cut(s) 684, 915, 1161
BstAPI GCANNNNNTGC 2 cut(s) 467, 1109
BstBAI YACGTR 1 cut(s) 1303
BstC8I GCNNGC 4 cut(s) 232, 367, 1046, 1254
BstDEI CTNAG 4 cut(s) 44, 611, 661, 728
BstF5I GGATG 1 cut(s) 1266
BstKTI GATC 3 cut(s) 349, 723, 831
BstMAI GTCTC 3 cut(s) 578, 927, 1262
BstMBI GATC 3 cut(s) 346, 720, 828
BstMWI GCNNNNNNNGC 8 cut(s) 40, 83, 467, 497, 896, 905, 1109, 1190
BstNI CCWGG 2 cut(s) 537, 1149
BstSCI CCNGG 3 cut(s) 535, 854, 1147
BstSFI CTRYAG 2 cut(s) 558, 918
BstSLI GKGCMC 2 cut(s) 395, 856
BstV1I GCAGC 6 cut(s) 316, 349, 394, 509, 544, 1204
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 828
BstYI RGATCY 1 cut(s) 828
BsuRI GGCC 4 cut(s) 77, 234, 369, 733
BtsCI GGATG 1 cut(s) 1266
BtsI GCAGTG 2 cut(s) 29, 180
BtsIMutI CAGTG 5 cut(s) 29, 180, 393, 856, 1062
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 4 cut(s) 232, 367, 1046, 1254
CaiI CAGNNNCTG 1 cut(s) 638
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 4 cut(s) 109, 177, 368, 650
Csp6I GTAC 4 cut(s) 636, 819, 839, 864
CspCI CAANNNNNGTGG 2 cut(s) 169, 204
CviAII CATG 3 cut(s) 359, 901, 1257
CviQI GTAC 4 cut(s) 636, 819, 839, 864
DdeI CTNAG 4 cut(s) 44, 611, 661, 728
DpnI GATC 3 cut(s) 348, 722, 830
DpnII GATC 3 cut(s) 346, 720, 828
DrdI GACNNNNNNGTC 1 cut(s) 977
DseDI GACNNNNNNGTC 1 cut(s) 977
EaeI YGGCCR 2 cut(s) 75, 232
Eam1104I CTCTTC 3 cut(s) 684, 915, 1161
EarI CTCTTC 3 cut(s) 684, 915, 1161
EciI GGCGGA 1 cut(s) 1063
Eco130I CCWWGG 1 cut(s) 832
Eco32I GATATC 1 cut(s) 304
Eco47I GGWCC 3 cut(s) 109, 177, 650
Eco57I CTGAAG 1 cut(s) 770
Eco88I CYCGRG 1 cut(s) 105
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 2 cut(s) 535, 1147
EcoRV GATATC 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 832
EcoT22I ATGCAT 1 cut(s) 463
ErhI CCWWGG 1 cut(s) 832
Esp3I CGTCTC 1 cut(s) 578
FaeI CATG 3 cut(s) 362, 904, 1260
FalI AAGNNNNNCTT 2 cut(s) 743, 775
FatI CATG 3 cut(s) 358, 900, 1256
FauI CCCGC 2 cut(s) 1014, 1037
Fnu4HI GCNGC 6 cut(s) 330, 363, 408, 498, 558, 1218
FokI GGATG 1 cut(s) 1273
Fsp4HI GCNGC 6 cut(s) 330, 363, 408, 498, 558, 1218
FspBI CTAG 5 cut(s) 99, 335, 566, 669, 833
GluI GCNGC 6 cut(s) 330, 363, 408, 498, 558, 1218
GsuI CTGGAG 2 cut(s) 1023, 1194
HaeIII GGCC 4 cut(s) 77, 234, 369, 733
HapII CCGG 3 cut(s) 366, 856, 1163
Hin1II CATG 3 cut(s) 362, 904, 1260
HindIII AAGCTT 1 cut(s) 1079
HpaII CCGG 3 cut(s) 366, 856, 1163
Hpy166II GTNNAC 2 cut(s) 180, 393
Hpy188I TCNGA 5 cut(s) 47, 173, 286, 983, 1278
Hpy188III TCNNGA 5 cut(s) 335, 482, 824, 1130, 1163
Hpy8I GTNNAC 2 cut(s) 180, 393
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 883
HpyCH4III ACNGT 3 cut(s) 397, 847, 1057
HpyCH4IV ACGT 2 cut(s) 1213, 1302
HpyF10VI GCNNNNNNNGC 8 cut(s) 40, 83, 467, 497, 896, 905, 1109, 1190
HpyF3I CTNAG 4 cut(s) 44, 611, 661, 728
HpySE526I ACGT 2 cut(s) 1213, 1302
Hsp92II CATG 3 cut(s) 362, 904, 1260
Kpn2I TCCGGA 1 cut(s) 1162
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 3 cut(s) 346, 720, 828
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 1 cut(s) 120
Lsp1109I GCAGC 6 cut(s) 316, 349, 394, 509, 544, 1204
LweI GCATC 1 cut(s) 331
MaeI CTAG 5 cut(s) 99, 335, 566, 669, 833
MaeII ACGT 2 cut(s) 1213, 1302
MaeIII GTNAC 2 cut(s) 1070, 1240
MalI GATC 3 cut(s) 348, 722, 830
MboI GATC 3 cut(s) 346, 720, 828
MfeI CAATTG 1 cut(s) 1185
MflI RGATCY 1 cut(s) 828
MhlI GDGCHC 3 cut(s) 153, 395, 856
MlsI TGGCCA 1 cut(s) 77
MluCI AATT 8 cut(s) 133, 219, 799, 883, 1179, 1185, 1234, 1296
MluNI TGGCCA 1 cut(s) 77
MmeI TCCRAC 7 cut(s) 151, 222, 303, 400, 677, 973, 1075
Mox20I TGGCCA 1 cut(s) 77
Mph1103I ATGCAT 1 cut(s) 463
MroI TCCGGA 1 cut(s) 1162
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 567
MscI TGGCCA 1 cut(s) 77
MseI TTAA 3 cut(s) 413, 705, 960
Msp20I TGGCCA 1 cut(s) 77
MspI CCGG 3 cut(s) 366, 856, 1163
MspR9I CCNGG 3 cut(s) 537, 856, 1149
MunI CAATTG 1 cut(s) 1185
Mva1269I GAATGC 2 cut(s) 461, 750
MvaI CCWGG 2 cut(s) 537, 1149
MwoI GCNNNNNNNGC 8 cut(s) 40, 83, 467, 497, 896, 905, 1109, 1190
NaeI GCCGGC 1 cut(s) 367
NciI CCSGG 1 cut(s) 856
NdeII GATC 3 cut(s) 346, 720, 828
NgoMIV GCCGGC 1 cut(s) 365
NlaIII CATG 3 cut(s) 362, 904, 1260
NlaIV GGNNCC 2 cut(s) 110, 178
NmuCI GTSAC 1 cut(s) 1070
NsiI ATGCAT 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 915
PctI GAATGC 2 cut(s) 461, 750
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 567
PflMI CCANNNNNTGG 2 cut(s) 740, 955
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 6 cut(s) 331, 364, 409, 499, 559, 1219
Ppu21I YACGTR 1 cut(s) 1303
Psp6I CCWGG 2 cut(s) 535, 1147
PspGI CCWGG 2 cut(s) 535, 1147
PspN4I GGNNCC 2 cut(s) 110, 178
PspPI GGNCC 4 cut(s) 109, 177, 368, 650
PstI CTGCAG 2 cut(s) 562, 922
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 828
RsaI GTAC 4 cut(s) 637, 820, 840, 865
RsaNI GTAC 4 cut(s) 636, 819, 839, 864
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 3 cut(s) 413, 705, 960
SatI GCNGC 6 cut(s) 330, 363, 408, 498, 558, 1218
Sau3AI GATC 3 cut(s) 346, 720, 828
Sau96I GGNCC 4 cut(s) 109, 177, 368, 650
ScaI AGTACT 1 cut(s) 820
ScrFI CCNGG 3 cut(s) 537, 856, 1149
SduI GDGCHC 3 cut(s) 153, 395, 856
SfaNI GCATC 1 cut(s) 331
SfcI CTRYAG 2 cut(s) 558, 918
SinI GGWCC 3 cut(s) 109, 177, 650
SmlI CTYRAG 1 cut(s) 1130
SmoI CTYRAG 1 cut(s) 1130
SpeI ACTAGT 3 cut(s) 98, 565, 668
Sse9I AATT 8 cut(s) 133, 219, 799, 883, 1179, 1185, 1234, 1296
SsiI CCGC 4 cut(s) 5, 1021, 1044, 1048
SspMI CTAG 5 cut(s) 99, 335, 566, 669, 833
StyD4I CCNGG 3 cut(s) 535, 854, 1147
StyI CCWWGG 1 cut(s) 832
TaaI ACNGT 3 cut(s) 397, 847, 1057
TaiI ACGT 2 cut(s) 1216, 1305
TaqI TCGA 2 cut(s) 137, 300
TasI AATT 8 cut(s) 133, 219, 799, 883, 1179, 1185, 1234, 1296
TatI WGTACW 1 cut(s) 818
Tru1I TTAA 3 cut(s) 413, 705, 960
Tru9I TTAA 3 cut(s) 413, 705, 960
TscAI CASTG 5 cut(s) 36, 187, 400, 856, 1062
TseFI GTSAC 1 cut(s) 1070
TseI GCWGC 6 cut(s) 329, 362, 407, 497, 557, 1217
Tsp45I GTSAC 1 cut(s) 1070
TspDTI ATGAA 2 cut(s) 327, 347
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 5 cut(s) 36, 187, 400, 856, 1062
Van91I CCANNNNNTGG 2 cut(s) 740, 955
VneI GTGCAC 1 cut(s) 391
VpaK11BI GGWCC 3 cut(s) 109, 177, 650
XapI RAATTY 4 cut(s) 133, 799, 1234, 1296
XbaI TCTAGA 1 cut(s) 334
XmaJI CCTAGG 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 5 cut(s) 99, 335, 566, 669, 833
ZrmI AGTACT 1 cut(s) 820
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.