Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010294

Physical Location & Seq

Genomic Coordinates Reverse (-)

18707924 .. 18716118

Gene Structure 8195 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G50500.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGGATAAGTCGAGTGCTTTAGAGTATATCAACCAGATGTTCCCAACAGAGGCATCGCTAACTGGCGTTGAGCCACTTATGCAAAAGATTCAGGGTGAAATTCGGCGGGTCGATGCTAGCATACTCTCTGCTGTTCGTCAACAGAGTAATTCGGGAACTAAAGCTAAAGAAGATCTTGCTGATGCTACGCGTGCTGTGGAGGAACTCTCTCATAAGATTCAAGAGATAAAATCCAAAGCTGAGCAAAGTGAAGCAATGGTTCAAGAAATATGCCGTGACATTAAGAAGTTAGATTTTGCAAAGAAGAACATAACCACGACAATCACTGCCCTTCACCGCCTCACAATGTTAGTCTCTGCTGTTGAACAGCTTCAGGTGATGGCGTCAAAAAGACAATACAAGGAGGCAGCTGCACAGCTCGAGGCTGTAAACCAATTGTGTAACCACTTTGAAGCATACAGGGATGTTCCTAAAATCACGGAGCTCAGAGAGAAGCTTAATAATATAAAGCAAATCCTTAAGTCCCATGTATTTTCTGATTTTTCCAGTTTAGGTACTGGGAAAGAGACAGAGGAAACAAATTTGCTACAGAAGTTATCTGATTCTTGCTTGGTTGTTGATGCTCTGGAACCATCTGTGAGGGAAGAGTTGGTAAATAACTTTTGCAGCAGGGAGCTCACCTCATATGAACAAATTTTTGAAGGAGCTGAATTGGCAAAGTTGGATAAGACAGAGCGGAGATATGCTTGGATAAAGCGTCGAATCCGAACAAATGAAGAAATTTGGAAGATTTTTCCTGCTTCTTGGCATGTGCCGTATAGGCTATGCATTCAGTTCTGCAAGCAAACAAGGAAGCAAGTAGAATCTATTCTGGTCAACATGAAAGAGAAGCCAGTTGTAGCGATATTGCTTCTGGCACTACAAAGTACAGTGGAGTTTGAAAAAGAACTTGAAAAGAAATTTGGTGGTGGTGTTCCTACTAAAGATATTGAAGATGACATTGAAGAAATTGGTACATGGGAAGATAATAGTCAGAATATATCCAAAATCCGCAAGAAATATGAAAAGAAGTTTGCTGCTAGTCAAGAAACCGAGGAGAATGGTTTTCAACAGGAAAAGACCGGAAACAAAGATTTATCTGTTACTGGAGCTGGGTTTAACTTCCGTGGAATGATCTCTTCCTGCTTTGAACCTCATTTAACTCCATATATTGAGTTGGAAGAGAAAACACTTATGGATGACCTGGAGAAAATTGTTCAGGAGGAATCATGGGATGTTGAGGATGGAAGCCAAAATAATGTTTTATCTAGTAGCACACAGCTATTTTCCAATATAAAGAAGAGCTTGAAACGCTGTAATACTCTCAGTAAGAACCAGACTTTATTCAATTTGTTCAAGGTCTTTCAACGAGTTTTAAAGGCCTATGCTACAAAGCTATTTTTTAAGCTCCCAAAAGGAGGCACTGGTATCGTTGCCGCAGCCACAGGCATGGATGGACAGATAAAGGTCTCGGAGAGAGATGAAAGGGTGATATGCTACATAGTTAATTCTGCTGAGTATTGCCACAAAACATCTGGTGAACTGGCAGAAAACGTCTCAGAAATTATTGACCCACATTATGCTGATGGTGTAGACATGTCAGAGGTCCAGGATGAATTTTCAGCCGTCATAACGAAAGCATTGGTAACGCTGGTCCTTGGACTTGAGACTAAATTTGACACAGAAATGGCAGTGATGACACGTGTTCCATGGAGCACACTTGAGAGTGTTGGTGACCAGTCTGGGTACGTGAATGGAATAAATACAGTCCTTAGCGGCAGCATACCTGTCCTCGGGAAACTTCTAACACCAGTTTACTTCCAGTTTTTCCTTGACAAGCTGGCATCATCCCTAGGACCACGGTTCTATGCTAATATTTTCAGATGCAAGCAACTATCCGAAACTGGAGCTCAACAGATGTTACTAGATACGCAAGCTGTGAAGTCGATTCTACTAGAAATTCCTTCCCTTGCCAGACAGACCTCAACTGCTGCCAGCTATTCCAAATTTGTGAGCCGTGAAATGAGCAGAGCTGAAGCACTCCTTAAGGTCATACTATCCCCAATCGACTCAGTGGCAGACACTTATCGTGCACTGTTTCCGGAGGGAACGCCCATGGAGTTTCAACGCATCTTAGAACTTAAGGGTCTTAAGAAAGCTGATCAGCAGAGTATACTAGATGATTTCAACAAGCATGGTCCAGGATTCACACAGCAGTCGGTTGCAGCAGCAATGCCACAACCAATGCCAACCCCACCTGCACCACCATTGGCAATCACTAATCCGGCTACAGCTGCAGGGTTCATAGCAAACAGCGAGGATGTTTTAACCAGAGCAGCTGCTCTTGGCAGAGGAGCTGCAAGCACTGGCTTCAAGAAGTTCATTGCTCTTACCGAAGCTGCCAAAGACCGCAAAGATGGGCCTTTAAGGAGACTCTTCAACGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000938 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005794 GO:0006810 GO:0006890 GO:0006950 GO:0006970 GO:0007009 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010256 GO:0010286 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032991 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps53_N

Protein Analysis

828

Amino Acids

92.53

Weight (kDa)

5.97

Isoelectric Point (pI)

43.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps53_N	PF04100	34 - 441	1.6e-148	Vps53, N-terminal
VPS53_C	PF16854	671 - 749	4.2e-14	Vacuolar protein sorting-associated protein 53 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50500 AT1G50500 AT1G50500 AT1G50500 AT1G50500
fragaria_vesca	FvH4_6g40130 FvH4_6g40130
malus_domestica	MD09G1135000.v1.1 MD17G1124000.v1.1
prunus_persica	Prupe.3G191700_v2.0.a1
pyrus_communis	pycom09g05610 pycom17g11540
rosa_chinensis	RchiOBHm_Chr2g0154761
rosa_laevigata	RLG00000020801
rosa_multiflora	Rmu_sc0001027.1_g000002
rosa_roxburghii	Rroxscaffold_2G00094120
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2BG521400 Rh2CG495700 Rh2DG531500
rosa_wichuraiana	Rw2G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2305
Acc36I	ACCTGC	1 cut(s)	2305
AccBSI	CCGCTC	1 cut(s)	736
AccI	GTMKAC	2 cut(s)	1632, 2212
AccII	CGCG	1 cut(s)	190
AccIII	TCCGGA	1 cut(s)	2140
AciI	CCGC	7 cut(s)	106, 337, 736, 1051, 1476, 1815, 2449
AcsI	RAATTY	9 cut(s)	99, 580, 693, 780, 959, 1655, 1712, 1998, 2045
AcuI	CTGAAG	2 cut(s)	356, 2094
AcvI	CACGTG	1 cut(s)	1742
AcyI	GRCGYC	1 cut(s)	383
AfaI	GTAC	4 cut(s)	556, 928, 1015, 1787
AfiI	CCNNNNNNNGG	3 cut(s)	49, 1457, 1832
AflII	CTTAAG	4 cut(s)	518, 2084, 2180, 2189
AflIII	ACRYGT	4 cut(s)	188, 1635, 1739, 1741
AjnI	CCWGG	3 cut(s)	1242, 1647, 2239
AjuI	GAANNNNNNNTTGG	2 cut(s)	945, 977
Alw21I	GWGCWC	5 cut(s)	486, 678, 1757, 1951, 2134
Alw26I	GTCTC	6 cut(s)	358, 560, 1513, 1600, 1700, 2464
Alw44I	GTGCAC	1 cut(s)	2130
Ama87I	CYCGRG	2 cut(s)	419, 1832
Aor13HI	TCCGGA	1 cut(s)	2140
AoxI	GGCC	2 cut(s)	1419, 2459
ApaLI	GTGCAC	1 cut(s)	2130
ApoI	RAATTY	9 cut(s)	99, 580, 693, 780, 959, 1655, 1712, 1998, 2045
Asp700I	GAANNNNTTC	2 cut(s)	369, 867
AspA2I	CCTAGG	1 cut(s)	1891
AspS9I	GGNCC	5 cut(s)	1645, 1693, 1895, 2237, 2459
AsuHPI	GGTGA	7 cut(s)	107, 326, 388, 670, 1540, 1589, 1784
AsuNHI	GCTAGC	1 cut(s)	116
AvaI	CYCGRG	2 cut(s)	419, 1832
AvaII	GGWCC	4 cut(s)	1645, 1693, 1895, 2237
AvrII	CCTAGG	1 cut(s)	1891
BaeGI	GKGCMC	1 cut(s)	2134
BanII	GRGCYC	3 cut(s)	486, 678, 1951
BbrPI	CACGTG	1 cut(s)	1742
Bbv12I	GWGCWC	5 cut(s)	486, 678, 1757, 1951, 2134
BccI	CCATC	6 cut(s)	373, 640, 1277, 1487, 1619, 2450
BceAI	ACGGC	4 cut(s)	258, 799, 1649, 2040
BcgI	CGANNNNNNTGC	2 cut(s)	1450, 1484
BciT130I	CCWGG	3 cut(s)	1244, 1649, 2241
BclI	TGATCA	1 cut(s)	2200
BcoDI	GTCTC	6 cut(s)	358, 560, 1513, 1600, 1700, 2464
BfaI	CTAG	7 cut(s)	117, 1080, 1308, 1892, 1964, 1994, 2216
BfmI	CTRYAG	3 cut(s)	587, 2328, 2334
BfrI	CTTAAG	4 cut(s)	518, 2084, 2180, 2189
BfuAI	ACCTGC	1 cut(s)	2305
BglI	GCCNNNNNGGC	1 cut(s)	820
BglII	AGATCT	1 cut(s)	172
BlnI	CCTAGG	1 cut(s)	1891
BlpI	GCTNAGC	1 cut(s)	240
Bme1390I	CCNGG	3 cut(s)	1244, 1649, 2241
Bme18I	GGWCC	4 cut(s)	1645, 1693, 1895, 2237
BmeT110I	CYCGRG	2 cut(s)	419, 1832
BmgT120I	GGNCC	5 cut(s)	1645, 1693, 1895, 2237, 2459
BmiI	GGNNCC	1 cut(s)	630
BmrFI	CCNGG	3 cut(s)	1244, 1649, 2241
BmrI	ACTGGG	1 cut(s)	567
BmsI	GCATC	7 cut(s)	62, 103, 172, 610, 1892, 1913, 2178
BmtI	GCTAGC	1 cut(s)	120
BmuI	ACTGGG	1 cut(s)	567
BplI	GAGNNNNNCTC	4 cut(s)	191, 223, 665, 697
BpmI	CTGGAG	3 cut(s)	1167, 1265, 1965
Bpu10I	CCTNAGC	1 cut(s)	1811
Bpu1102I	GCTNAGC	1 cut(s)	240
BpuEI	CTTGAG	2 cut(s)	1724, 1781
BsaAI	YACGTR	2 cut(s)	1742, 1789
BsaHI	GRCGYC	1 cut(s)	383
BsaI	GGTCTC	1 cut(s)	1513
BsaJI	CCNNGG	8 cut(s)	1092, 1165, 1696, 1748, 1831, 1891, 1898, 2154
BsaWI	WCCGGW	2 cut(s)	1121, 2140
Bsc4I	CCNNNNNNNGG	3 cut(s)	49, 1457, 1832
Bse3DI	GCAATG	3 cut(s)	261, 2277, 2421
BseAI	TCCGGA	1 cut(s)	2140
BseBI	CCWGG	3 cut(s)	1244, 1649, 2241
BseDI	CCNNGG	8 cut(s)	1092, 1165, 1696, 1748, 1831, 1891, 1898, 2154
BseGI	GGATG	8 cut(s)	469, 1243, 1279, 1288, 1498, 1657, 1886, 2365
BseLI	CCNNNNNNNGG	3 cut(s)	49, 1457, 1832
BseMI	GCAATG	3 cut(s)	261, 2277, 2421
BseMII	CTCAG	6 cut(s)	231, 499, 1378, 1545, 1611, 2124
BseRI	GAGGAG	2 cut(s)	1109, 2406
BseSI	GKGCMC	1 cut(s)	2134
BseYI	CCCAGC	1 cut(s)	1151
BsgI	GTGCAG	2 cut(s)	396, 2283
Bsh1236I	CGCG	1 cut(s)	190
BshFI	GGCC	2 cut(s)	1421, 2461
BsiHKAI	GWGCWC	5 cut(s)	486, 678, 1757, 1951, 2134
BsiHKCI	CYCGRG	2 cut(s)	419, 1832
BsiSI	CCGG	3 cut(s)	1122, 2141, 2324
BslFI	GGGAC	1 cut(s)	508
BslI	CCNNNNNNNGG	3 cut(s)	49, 1457, 1832
BsmAI	GTCTC	6 cut(s)	358, 560, 1513, 1600, 1700, 2464
BsmBI	CGTCTC	1 cut(s)	1600
BsmFI	GGGAC	1 cut(s)	508
BsmI	GAATGC	1 cut(s)	828
BsnI	GGCC	2 cut(s)	1421, 2461
Bso31I	GGTCTC	1 cut(s)	1513
BsoBI	CYCGRG	2 cut(s)	419, 1832
Bsp1286I	GDGCHC	5 cut(s)	486, 678, 1757, 1951, 2134
Bsp13I	TCCGGA	1 cut(s)	2140
Bsp143I	GATC	3 cut(s)	172, 1173, 2200
Bsp1720I	GCTNAGC	1 cut(s)	240
Bsp19I	CCATGG	2 cut(s)	1748, 2154
BspACI	CCGC	7 cut(s)	106, 337, 736, 1051, 1476, 1815, 2449
BspANI	GGCC	2 cut(s)	1421, 2461
BspCNI	CTCAG	6 cut(s)	232, 498, 1377, 1546, 1610, 2123
BspEI	TCCGGA	1 cut(s)	2140
BspFNI	CGCG	1 cut(s)	190
BspLI	GGNNCC	1 cut(s)	630
BspMAI	CTGCAG	1 cut(s)	2338
BspMI	ACCTGC	1 cut(s)	2305
BspOI	GCTAGC	1 cut(s)	120
BspQI	GCTCTTC	1 cut(s)	1334
BspTI	CTTAAG	4 cut(s)	518, 2084, 2180, 2189
BspTNI	GGTCTC	1 cut(s)	1513
BsrBI	CCGCTC	1 cut(s)	736
BsrDI	GCAATG	3 cut(s)	261, 2277, 2421
BssECI	CCNNGG	8 cut(s)	1092, 1165, 1696, 1748, 1831, 1891, 1898, 2154
BssMI	GATC	3 cut(s)	172, 1173, 2200
BssNAI	GTATAC	1 cut(s)	2213
BssNI	GRCGYC	1 cut(s)	383
BssT1I	CCWWGG	4 cut(s)	1696, 1748, 1891, 2154
Bst1107I	GTATAC	1 cut(s)	2213
Bst2UI	CCWGG	3 cut(s)	1244, 1649, 2241
Bst4CI	ACNGT	4 cut(s)	931, 1807, 1902, 2136
Bst6I	CTCTTC	5 cut(s)	639, 1183, 1215, 1334, 2480
BstACI	GRCGYC	1 cut(s)	383
BstAFI	CTTAAG	4 cut(s)	518, 2084, 2180, 2189
BstBAI	YACGTR	2 cut(s)	1742, 1789
BstC8I	GCNNGC	8 cut(s)	118, 192, 842, 1881, 1928, 1974, 2035, 2401
BstDEI	CTNAG	8 cut(s)	240, 485, 1364, 1554, 1597, 1811, 2110, 2173
BstDSI	CCRYGG	4 cut(s)	1165, 1748, 1898, 2154
BstEII	GGTNACC	1 cut(s)	1772
BstF5I	GGATG	8 cut(s)	469, 1243, 1279, 1288, 1498, 1657, 1886, 2365
BstFNI	CGCG	1 cut(s)	190
BstKTI	GATC	3 cut(s)	175, 1176, 2203
BstMAI	GTCTC	6 cut(s)	358, 560, 1513, 1600, 1700, 2464
BstMBI	GATC	3 cut(s)	172, 1173, 2200
BstMWI	GCNNNNNNNGC	6 cut(s)	79, 191, 713, 820, 1350, 2333
BstNI	CCWGG	3 cut(s)	1244, 1649, 2241
BstNSI	RCATGY	2 cut(s)	812, 1639
BstPI	GGTNACC	1 cut(s)	1772
BstSCI	CCNGG	3 cut(s)	1242, 1647, 2239
BstSFI	CTRYAG	3 cut(s)	587, 2328, 2334
BstSLI	GKGCMC	1 cut(s)	2134
BstUI	CGCG	1 cut(s)	190
BstX2I	RGATCY	1 cut(s)	172
BstXI	CCANNNNNNTGG	1 cut(s)	1489
BstYI	RGATCY	1 cut(s)	172
BstZ17I	GTATAC	1 cut(s)	2213
BsuRI	GGCC	2 cut(s)	1421, 2461
BtgI	CCRYGG	4 cut(s)	1165, 1748, 1898, 2154
BtgZI	GCGATG	1 cut(s)	39
BtsCI	GGATG	8 cut(s)	469, 1243, 1279, 1288, 1498, 1657, 1886, 2365
BtsI	GCAGTG	2 cut(s)	324, 1737
BtsIMutI	CAGTG	7 cut(s)	324, 936, 1461, 1737, 2118, 2132, 2403
BveI	ACCTGC	1 cut(s)	2305
Cac8I	GCNNGC	8 cut(s)	118, 192, 842, 1881, 1928, 1974, 2035, 2401
Cfr13I	GGNCC	5 cut(s)	1645, 1693, 1895, 2237, 2459
CseI	GACGC	2 cut(s)	372, 746
Csp6I	GTAC	4 cut(s)	555, 927, 1014, 1786
CviQI	GTAC	4 cut(s)	555, 927, 1014, 1786
DdeI	CTNAG	8 cut(s)	240, 485, 1364, 1554, 1597, 1811, 2110, 2173
DpnI	GATC	3 cut(s)	174, 1175, 2202
DpnII	GATC	3 cut(s)	172, 1173, 2200
DraI	TTTAAA	1 cut(s)	1416
Eam1104I	CTCTTC	5 cut(s)	639, 1183, 1215, 1334, 2480
EarI	CTCTTC	5 cut(s)	639, 1183, 1215, 1334, 2480
Ecl136II	GAGCTC	3 cut(s)	484, 676, 1949
Eco130I	CCWWGG	4 cut(s)	1696, 1748, 1891, 2154
Eco147I	AGGCCT	1 cut(s)	1421
Eco24I	GRGCYC	3 cut(s)	486, 678, 1951
Eco31I	GGTCTC	1 cut(s)	1513
Eco47I	GGWCC	4 cut(s)	1645, 1693, 1895, 2237
Eco53kI	GAGCTC	3 cut(s)	484, 676, 1949
Eco57I	CTGAAG	2 cut(s)	356, 2094
Eco72I	CACGTG	1 cut(s)	1742
Eco88I	CYCGRG	2 cut(s)	419, 1832
Eco91I	GGTNACC	1 cut(s)	1772
EcoICRI	GAGCTC	3 cut(s)	484, 676, 1949
EcoO65I	GGTNACC	1 cut(s)	1772
EcoRII	CCWGG	3 cut(s)	1242, 1647, 2239
EcoT14I	CCWWGG	4 cut(s)	1696, 1748, 1891, 2154
EcoT22I	ATGCAT	1 cut(s)	830
EcoT38I	GRGCYC	3 cut(s)	486, 678, 1951
ErhI	CCWWGG	4 cut(s)	1696, 1748, 1891, 2154
Esp3I	CGTCTC	1 cut(s)	1600
FalI	AAGNNNNNCTT	6 cut(s)	159, 191, 1328, 1360, 2067, 2099
FaqI	GGGAC	1 cut(s)	508
FauI	CCCGC	1 cut(s)	99
FauNDI	CATATG	1 cut(s)	685
FbaI	TGATCA	1 cut(s)	2200
FblI	GTMKAC	2 cut(s)	1632, 2212
FokI	GGATG	8 cut(s)	476, 1250, 1286, 1295, 1505, 1664, 1873, 2372
FriOI	GRGCYC	3 cut(s)	486, 678, 1951
FspBI	CTAG	7 cut(s)	117, 1080, 1308, 1892, 1964, 1994, 2216
GsaI	CCCAGC	1 cut(s)	1155
GsuI	CTGGAG	3 cut(s)	1167, 1265, 1965
HaeIII	GGCC	2 cut(s)	1421, 2461
HapII	CCGG	3 cut(s)	1122, 2141, 2324
HgaI	GACGC	2 cut(s)	372, 746
Hin1I	GRCGYC	1 cut(s)	383
HincII	GTYRAC	2 cut(s)	140, 877
HindII	GTYRAC	2 cut(s)	140, 877
HindIII	AAGCTT	1 cut(s)	494
HpaII	CCGG	3 cut(s)	1122, 2141, 2324
HphI	GGTGA	7 cut(s)	107, 326, 388, 670, 1540, 1589, 1784
Hpy166II	GTNNAC	8 cut(s)	140, 430, 877, 1580, 1633, 1855, 2132, 2213
Hpy188III	TCNNGA	9 cut(s)	153, 221, 263, 626, 1085, 1259, 1834, 2141, 2413
Hpy8I	GTNNAC	8 cut(s)	140, 430, 877, 1580, 1633, 1855, 2132, 2213
Hpy99I	CGWCG	1 cut(s)	762
HpyAV	CCTTC	3 cut(s)	341, 695, 2013
HpyCH4III	ACNGT	4 cut(s)	931, 1807, 1902, 2136
HpyCH4IV	ACGT	3 cut(s)	1593, 1741, 1788
HpyF10VI	GCNNNNNNNGC	6 cut(s)	79, 191, 713, 820, 1350, 2333
HpyF3I	CTNAG	8 cut(s)	240, 485, 1364, 1554, 1597, 1811, 2110, 2173
HpySE526I	ACGT	3 cut(s)	1593, 1741, 1788
Hsp92I	GRCGYC	1 cut(s)	383
Kpn2I	TCCGGA	1 cut(s)	2140
Ksp22I	TGATCA	1 cut(s)	2200
Kzo9I	GATC	3 cut(s)	172, 1173, 2200
LguI	GCTCTTC	1 cut(s)	1334
LmnI	GCTCC	8 cut(s)	481, 673, 704, 1148, 1452, 1752, 1946, 2393
LweI	GCATC	7 cut(s)	62, 103, 172, 610, 1892, 1913, 2178
MaeI	CTAG	7 cut(s)	117, 1080, 1308, 1892, 1964, 1994, 2216
MaeII	ACGT	3 cut(s)	1593, 1741, 1788
MaeIII	GTNAC	6 cut(s)	275, 440, 1141, 1684, 1772, 1959
MalI	GATC	3 cut(s)	174, 1175, 2202
MbiI	CCGCTC	1 cut(s)	736
MboI	GATC	3 cut(s)	172, 1173, 2200
MfeI	CAATTG	1 cut(s)	434
MflI	RGATCY	1 cut(s)	172
MhlI	GDGCHC	5 cut(s)	486, 678, 1757, 1951, 2134
MluI	ACGCGT	1 cut(s)	188
MlyI	GAGTC	2 cut(s)	2102, 2466
MmeI	TCCRAC	2 cut(s)	702, 1197
Mph1103I	ATGCAT	1 cut(s)	830
MroI	TCCGGA	1 cut(s)	2140
MroXI	GAANNNNTTC	2 cut(s)	369, 867
MslI	CAYNNNNRTG	2 cut(s)	1487, 1724
MspA1I	CMGCKG	3 cut(s)	410, 2333, 2378
MspCI	CTTAAG	4 cut(s)	518, 2084, 2180, 2189
MspI	CCGG	3 cut(s)	1122, 2141, 2324
MspR9I	CCNGG	3 cut(s)	1244, 1649, 2241
MunI	CAATTG	1 cut(s)	434
Mva1269I	GAATGC	1 cut(s)	828
MvaI	CCWGG	3 cut(s)	1244, 1649, 2241
MvnI	CGCG	1 cut(s)	190
MwoI	GCNNNNNNNGC	6 cut(s)	79, 191, 713, 820, 1350, 2333
NcoI	CCATGG	2 cut(s)	1748, 2154
NdeI	CATATG	1 cut(s)	685
NdeII	GATC	3 cut(s)	172, 1173, 2200
NheI	GCTAGC	1 cut(s)	116
NlaIV	GGNNCC	1 cut(s)	630
NmuCI	GTSAC	2 cut(s)	275, 1772
NsiI	ATGCAT	1 cut(s)	830
NspI	RCATGY	2 cut(s)	812, 1639
PaeR7I	CTCGAG	1 cut(s)	419
PaqCI	CACCTGC	1 cut(s)	2305
PceI	AGGCCT	1 cut(s)	1421
PciI	ACATGT	1 cut(s)	1635
PciSI	GCTCTTC	1 cut(s)	1334
PctI	GAATGC	1 cut(s)	828
PdmI	GAANNNNTTC	2 cut(s)	369, 867
PfeI	GAWTC	8 cut(s)	88, 217, 602, 762, 863, 1265, 1987, 2244
PfoI	TCCNGGA	2 cut(s)	1647, 2239
PleI	GAGTC	2 cut(s)	2102, 2466
PmaCI	CACGTG	1 cut(s)	1742
PmlI	CACGTG	1 cut(s)	1742
PpsI	GAGTC	2 cut(s)	2102, 2466
Ppu21I	YACGTR	2 cut(s)	1742, 1789
PscI	ACATGT	1 cut(s)	1635
Psp124BI	GAGCTC	3 cut(s)	486, 678, 1951
Psp6I	CCWGG	3 cut(s)	1242, 1647, 2239
PspCI	CACGTG	1 cut(s)	1742
PspEI	GGTNACC	1 cut(s)	1772
PspFI	CCCAGC	1 cut(s)	1151
PspGI	CCWGG	3 cut(s)	1242, 1647, 2239
PspN4I	GGNNCC	1 cut(s)	630
PspPI	GGNCC	5 cut(s)	1645, 1693, 1895, 2237, 2459
PspXI	VCTCGAGB	1 cut(s)	419
PstI	CTGCAG	1 cut(s)	2338
PsuI	RGATCY	1 cut(s)	172
PvuII	CAGCTG	3 cut(s)	410, 2333, 2378
RsaI	GTAC	4 cut(s)	556, 928, 1015, 1787
RsaNI	GTAC	4 cut(s)	555, 927, 1014, 1786
RseI	CAYNNNNRTG	2 cut(s)	1487, 1724
SacI	GAGCTC	3 cut(s)	486, 678, 1951
SapI	GCTCTTC	1 cut(s)	1334
Sau3AI	GATC	3 cut(s)	172, 1173, 2200
Sau96I	GGNCC	5 cut(s)	1645, 1693, 1895, 2237, 2459
SchI	GAGTC	2 cut(s)	2102, 2466
ScrFI	CCNGG	3 cut(s)	1244, 1649, 2241
SduI	GDGCHC	5 cut(s)	486, 678, 1757, 1951, 2134
SfaNI	GCATC	7 cut(s)	62, 103, 172, 610, 1892, 1913, 2178
SfcI	CTRYAG	3 cut(s)	587, 2328, 2334
Sfr274I	CTCGAG	1 cut(s)	419
SinI	GGWCC	4 cut(s)	1645, 1693, 1895, 2237
SlaI	CTCGAG	1 cut(s)	419
SmiMI	CAYNNNNRTG	2 cut(s)	1487, 1724
SmlI	CTYRAG	7 cut(s)	419, 518, 1703, 1760, 2084, 2180, 2189
SmoI	CTYRAG	7 cut(s)	419, 518, 1703, 1760, 2084, 2180, 2189
SseBI	AGGCCT	1 cut(s)	1421
SsiI	CCGC	7 cut(s)	106, 337, 736, 1051, 1476, 1815, 2449
SspI	AATATT	1 cut(s)	1915
SspMI	CTAG	7 cut(s)	117, 1080, 1308, 1892, 1964, 1994, 2216
SstI	GAGCTC	3 cut(s)	486, 678, 1951
StuI	AGGCCT	1 cut(s)	1421
StyD4I	CCNGG	3 cut(s)	1242, 1647, 2239
StyI	CCWWGG	4 cut(s)	1696, 1748, 1891, 2154
TaaI	ACNGT	4 cut(s)	931, 1807, 1902, 2136
TaiI	ACGT	3 cut(s)	1596, 1744, 1791
TaqI	TCGA	6 cut(s)	11, 111, 420, 760, 1985, 2106
TatI	WGTACW	1 cut(s)	926
TauI	GCSGC	2 cut(s)	1478, 1818
TfiI	GAWTC	8 cut(s)	88, 217, 602, 762, 863, 1265, 1987, 2244
TscAI	CASTG	7 cut(s)	331, 936, 1468, 1737, 2118, 2139, 2410
TseFI	GTSAC	2 cut(s)	275, 1772
Tsp45I	GTSAC	2 cut(s)	275, 1772
TspDTI	ATGAA	8 cut(s)	702, 789, 896, 1077, 1536, 1668, 2332, 2410
TspGWI	ACGGA	2 cut(s)	494, 1154
TspRI	CASTG	7 cut(s)	331, 936, 1468, 1737, 2118, 2139, 2410
Vha464I	CTTAAG	4 cut(s)	518, 2084, 2180, 2189
VneI	GTGCAC	1 cut(s)	2130
VpaK11BI	GGWCC	4 cut(s)	1645, 1693, 1895, 2237
XapI	RAATTY	9 cut(s)	99, 580, 693, 780, 959, 1655, 1712, 1998, 2045
XceI	RCATGY	2 cut(s)	812, 1639
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1571
XhoI	CTCGAG	1 cut(s)	419
XmaJI	CCTAGG	1 cut(s)	1891
XmiI	GTMKAC	2 cut(s)	1632, 2212
XmnI	GAANNNNTTC	2 cut(s)	369, 867
XspI	CTAG	7 cut(s)	117, 1080, 1308, 1892, 1964, 1994, 2216
Zsp2I	ATGCAT	1 cut(s)	830

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.