Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0010294

Physical Location & Seq

Genomic Coordinates Reverse (-)

10768728 .. 10777964

Gene Structure 9237 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1124000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2469 bp

ATGGATAAGTCAAGCACTTTGGAATACATCAACCAGATGTTCCCCACAGAGGCGTCTTTATCTGGTGTGGAGCCGCTAATGCAAAAGATACACGGTGAGATTCGGCGAGTAGATGCTGGAATTTTAGCCGCGGTTCGCCAGCAGAGCAATTCGGGCATGAAAGCAAAAGAAGATCTTGCTGCCGCTACACGTGCCGTGGAGGAACTCATGTATAAAATCCGCGAAATTAAAACGAAAGCTGAGCAAAGCGAGACAATGGTTCAAGAAATATGTAGGGATATTAAGAAGTTAGACTCTGCAAAGAAGCATATAACAACGACGATTACTGCTCTTCATCGCCTAACCATGCTTGTCTCTGCTGTTGAGCAGCTTCAAGTAATGGCTTCGAAACGACAATATAAAAAGGCAGCCGCACAGTTGGAGGCAGTGAACCAGTTATGTAGCCATTTTGAAGCTTATCAGGACATTCCGAAGATCACAGAGCTGAGGGAAAAATTTAAGAATATCAAACAAATACTAAAGTCGCACGTGTTTTCTGATTTCTCAAGCTTAGGTACTGGAAAGGAGACGGAGGAAACAAATTTACTTCAGCAGTTATCTGATGCTTGCCTGGTTGTTGATGCTTTGGAGCCATCTGTGAGGGAAGAACTAGTAAATAACTTTTGCAGCAGGGAGCTTACTTCATATGAGCAGATATTTGAAGGAGCTGAACTAGCTAAGTTGGATAAAACTGAACGAAGATATGCATGGATCAAGCGTCGTATGAGAACAAATGAAGAAATTTGGAAAATCTTTCCTAATTCATGGCATGTTCCTTATCGGCTCTGTATTCAGTTCTGTAAAAAAACAAGAAAGCAGCTGGAAGATATACATAATAATCAGAAAGAAAATCCTGATGTTGGCATATTATTGTTGGCACTACAGCGAACTCTTGAATTTGAGGATGAATTGGCAGAGAAATTTGGTGGTGGTACTCGAGGCAGGGAGATCGGAAATGAGATTGAAGAAATTGGTAGAGGGGAAAACTCTAGTCAAAGTGCCTCAGATATTAGAAAGAAGTATGAAAAGAAGCTTGCTGCACATCAGGAAAGTAGCACAGAGGAAAAGGATAAAGAGTTATCAGCTCCTGCAGCTGGGTTCAACTTCCGTGGAATCATTTCATCTTGCTTTGAGCCTCACTTGAATGTTTACACAGAACTAGAAGAGAAGACACTGATGGAGAATTTGGAGAAACTTGTTCAGGAGGAAACATGGGACATTGAGGAGGGAAGTCAGAATAATGTTTTATCAAGTAGCATGCAGTTGTTTCTTATAATCAAGAGGAGCTTAAAGAGATGCAGTGCTTTGACAAAGAACCAGACATTATTTAATTTGTTCAAGGTGTTTCAAAGGATTCTCAAAGGATATGCTACCAAGCTTTTTGCAAGAATACCTAAAGGTGGTACAGGAATTGTTGCAGCAGCAACTGGTATGGATGGACAGATAAAGACATCTGACAGAGATGAAAAGGTGATTTGTTACATTGTCAACTCGGCAGAATACTGCCATAAGACGTCTGGTGAACTGGCTGAAAGTGTATCCAAAATAATTGATACTCAGTTTGCTGATGGGGTGGATATGTCGGAAGTGCAGGATGAATTTTCAGCCGTTATAACAAAAGCATTGGTAACCTTGGTGCATGGCCTAGAAACTAAGTTTGATGCTGAAATGGCTGCAATGACACGAGTTCCATGGGCTACGCTTGAAAGTGTTGGTGACCAATCAGAGTATGTTAATGGCATAAATATGATCCTTGCTAGTAGTATTCCTATACTTGGAACACTTCTCTCACCAATTTACTTCCAATTTTTCTTGGACAAGCTTGCATCATCTCTTGGTCCGCGTTTCTATACTAACATTTTCAAATGCAAGCAAATATCAGAAACTGGAGCTCAACAAATGCTACTGGATACTCAAGCTGTAAAGACAATTCTTTTGGAGATACCATCCCTTGGTCACCAAACTTCACGTGCTGCTAGCTATTCGAAATTTGTAAGCCGGGAGATGAGCAAAGCTGAAGCACTTCTGAAGGTCATTCTGTCGCCAATTGATTCTGTTGCGGATACATATCGTGCGTTGCTACCAGAGGGAACGCCCATGGAGTTTCAGAGAATTTTGGAGCTCAAGGGTCTTAAGAAAGCAGATCAGCAAAGCATACTAGATGACTTTAACAAGCATGGCCCGGGAATCACAAAACCTGCTCTCCCACCCCCGGCTGCCCCAACTATCCCACTCCCAACTGCCCCCGCAGCTGCTCTGATACCAAATCCTACTTCAGCCGCCCTTATTGCATCCAGAGACGATGTGCTAACCAGAGCAGCTGCACTCGGGCGAGGTGCAGCCACAACAGGGTTCAAGAGGTTCCTGGCGTTAACCGAAGCTGCAAAAGATAGAAAAGACGGGCCTTTCAGAAAGCTCTTCAACGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000938 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005794 GO:0006810 GO:0006890 GO:0006950 GO:0006970 GO:0007009 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010256 GO:0010286 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032991 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps53_N

Protein Analysis

823

Amino Acids

91.79

Weight (kDa)

6.72

Isoelectric Point (pI)

39.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps53_N	PF04100	3 - 416	7e-142	Vps53, N-terminal
VPS53_C	PF16854	646 - 724	5.9e-14	Vacuolar protein sorting-associated protein 53 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50500 AT1G50500 AT1G50500 AT1G50500 AT1G50500
fragaria_vesca	FvH4_6g40130 FvH4_6g40130
malus_domestica	MD09G1135000.v1.1 MD17G1124000.v1.1
prunus_persica	Prupe.3G191700_v2.0.a1
pyrus_communis	pycom09g05610 pycom17g11540
rosa_chinensis	RchiOBHm_Chr2g0154761
rosa_laevigata	RLG00000020801
rosa_multiflora	Rmu_sc0001027.1_g000002
rosa_roxburghii	Rroxscaffold_2G00094120
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2BG521400 Rh2CG495700 Rh2DG531500
rosa_wichuraiana	Rw2G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1313, 1652
AatII	GACGTC	1 cut(s)	1556
Acc36I	ACCTGC	1 cut(s)	2245
AccB7I	CCANNNNNTGG	1 cut(s)	1991
AccII	CGCG	3 cut(s)	131, 222, 1882
AclWI	GGATC	2 cut(s)	758, 1783
AcuI	CTGAAG	4 cut(s)	572, 2076, 2087, 2298
AcvI	CACGTG	3 cut(s)	191, 529, 2009
AcyI	GRCGYC	2 cut(s)	53, 1553
AfaI	GTAC	3 cut(s)	556, 973, 1444
AfiI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2251, 2388
AflII	CTTAAG	1 cut(s)	2171
AflIII	ACRYGT	2 cut(s)	188, 528
AhlI	ACTAGT	1 cut(s)	649
AjnI	CCWGG	2 cut(s)	609, 2403
AjuI	GAANNNNNNNTTGG	2 cut(s)	2296, 2328
Alw21I	GWGCWC	2 cut(s)	1933, 2163
Alw26I	GTCTC	4 cut(s)	245, 358, 560, 2331
AlwI	GGATC	2 cut(s)	758, 1783
AlwNI	CAGNNNCTG	3 cut(s)	1127, 1466, 1925
Ama87I	CYCGRG	3 cut(s)	975, 2221, 2366
AoxI	GGCC	3 cut(s)	1681, 2218, 2441
ArsI	GACNNNNNNTTYG	2 cut(s)	1957, 1989
Asp700I	GAANNNNTTC	2 cut(s)	1156, 2061
AspS9I	GGNCC	3 cut(s)	1877, 2219, 2441
AsuC2I	CCSGG	4 cut(s)	2039, 2222, 2223, 2252
AsuHPI	GGTGA	6 cut(s)	107, 1522, 1571, 1766, 1821, 1988
AsuII	TTCGAA	2 cut(s)	386, 2024
AsuNHI	GCTAGC	1 cut(s)	2015
AvaI	CYCGRG	3 cut(s)	975, 2221, 2366
AvaII	GGWCC	1 cut(s)	1877
BanII	GRGCYC	2 cut(s)	1933, 2163
BauI	CACGAG	1 cut(s)	1722
BbrPI	CACGTG	3 cut(s)	191, 529, 2009
BbsI	GAAGAC	1 cut(s)	1214
Bbv12I	GWGCWC	2 cut(s)	1933, 2163
BbvCI	CCTCAGC	1 cut(s)	485
BccI	CCATC	5 cut(s)	640, 1210, 1469, 1601, 1993
BceAI	ACGGC	2 cut(s)	179, 1631
BcgI	CGANNNNNNTGC	2 cut(s)	970, 1004
BciT130I	CCWGG	2 cut(s)	611, 2405
BciVI	GTATCC	3 cut(s)	1588, 1942, 2095
BcnI	CCSGG	4 cut(s)	2039, 2222, 2223, 2252
BcoDI	GTCTC	4 cut(s)	245, 358, 560, 2331
BcuI	ACTAGT	1 cut(s)	649
BfaI	CTAG	8 cut(s)	650, 713, 1029, 1199, 1685, 1797, 2016, 2198
BfmI	CTRYAG	2 cut(s)	920, 1128
BfrI	CTTAAG	1 cut(s)	2171
BfuAI	ACCTGC	1 cut(s)	2245
BfuI	GTATCC	3 cut(s)	1588, 1942, 2095
BglII	AGATCT	1 cut(s)	172
BlpI	GCTNAGC	1 cut(s)	240
Bme1390I	CCNGG	6 cut(s)	611, 2039, 2222, 2223, 2252, 2405
Bme18I	GGWCC	1 cut(s)	1877
BmeT110I	CYCGRG	3 cut(s)	975, 2221, 2366
BmgT120I	GGNCC	3 cut(s)	1877, 2219, 2441
BmiI	GGNNCC	3 cut(s)	72, 630, 2402
BmrFI	CCNGG	6 cut(s)	611, 2039, 2222, 2223, 2252, 2405
BmsI	GCATC	7 cut(s)	103, 592, 610, 1325, 1690, 1874, 2339
BmtI	GCTAGC	1 cut(s)	2019
BpiI	GAAGAC	1 cut(s)	1214
BpmI	CTGGAG	1 cut(s)	1947
Bpu10I	CCTNAGC	2 cut(s)	485, 550
Bpu1102I	GCTNAGC	1 cut(s)	240
Bpu14I	TTCGAA	2 cut(s)	386, 2024
BpuEI	CTTGAG	3 cut(s)	529, 1938, 2147
BpuMI	CCSGG	4 cut(s)	2039, 2222, 2223, 2252
BsaAI	YACGTR	3 cut(s)	191, 529, 2009
BsaBI	GATNNNNATC	1 cut(s)	2106
BsaHI	GRCGYC	2 cut(s)	53, 1553
BsaJI	CCNNGG	9 cut(s)	129, 195, 1147, 1671, 1730, 1990, 2136, 2221, 2250
BsaXI	ACNNNNNCTCC	2 cut(s)	2226, 2256
Bsc4I	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2251, 2388
Bse1I	ACTGG	6 cut(s)	433, 562, 1471, 1569, 1930, 1950
Bse3DI	GCAATG	1 cut(s)	1722
Bse8I	GATNNNNATC	1 cut(s)	2106
BseBI	CCWGG	2 cut(s)	611, 2405
BseDI	CCNNGG	9 cut(s)	129, 195, 1147, 1671, 1730, 1990, 2136, 2221, 2250
BseGI	GGATG	5 cut(s)	949, 1480, 1639, 1985, 2330
BseJI	GATNNNNATC	1 cut(s)	2106
BseLI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2251, 2388
BseMI	GCAATG	1 cut(s)	1722
BseMII	CTCAG	4 cut(s)	231, 476, 1056, 1610
BseNI	ACTGG	6 cut(s)	433, 562, 1471, 1569, 1930, 1950
BseRI	GAGGAG	2 cut(s)	1277, 1336
BseYI	CCCAGC	1 cut(s)	1133
BsgI	GTGCAG	4 cut(s)	1062, 1649, 2346, 2397
Bsh1236I	CGCG	3 cut(s)	131, 222, 1882
BshFI	GGCC	3 cut(s)	1683, 2220, 2443
BsiHKAI	GWGCWC	2 cut(s)	1933, 2163
BsiHKCI	CYCGRG	3 cut(s)	975, 2221, 2366
BsiSI	CCGG	3 cut(s)	2038, 2222, 2252
BslFI	GGGAC	1 cut(s)	1268
BslI	CCNNNNNNNGG	8 cut(s)	49, 899, 1133, 1439, 1814, 1991, 2251, 2388
BsmAI	GTCTC	4 cut(s)	245, 358, 560, 2331
BsmBI	CGTCTC	2 cut(s)	560, 2331
BsmFI	GGGAC	1 cut(s)	1268
BsnI	GGCC	3 cut(s)	1683, 2220, 2443
BsoBI	CYCGRG	3 cut(s)	975, 2221, 2366
Bsp119I	TTCGAA	2 cut(s)	386, 2024
Bsp1286I	GDGCHC	2 cut(s)	1933, 2163
Bsp143I	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
Bsp1720I	GCTNAGC	1 cut(s)	240
Bsp19I	CCATGG	2 cut(s)	1730, 2136
BspANI	GGCC	3 cut(s)	1683, 2220, 2443
BspCNI	CTCAG	4 cut(s)	232, 477, 1055, 1609
BspFNI	CGCG	3 cut(s)	131, 222, 1882
BspLI	GGNNCC	3 cut(s)	72, 630, 2402
BspMAI	CTGCAG	1 cut(s)	1132
BspMI	ACCTGC	1 cut(s)	2245
BspOI	GCTAGC	1 cut(s)	2019
BspPI	GGATC	2 cut(s)	758, 1783
BspQI	GCTCTTC	2 cut(s)	336, 2462
BspT104I	TTCGAA	2 cut(s)	386, 2024
BspTI	CTTAAG	1 cut(s)	2171
BsrDI	GCAATG	1 cut(s)	1722
BsrI	ACTGG	6 cut(s)	433, 562, 1471, 1569, 1930, 1950
BssECI	CCNNGG	9 cut(s)	129, 195, 1147, 1671, 1730, 1990, 2136, 2221, 2250
BssMI	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
BssNI	GRCGYC	2 cut(s)	53, 1553
BssSI	CACGAG	1 cut(s)	1722
BssT1I	CCWWGG	4 cut(s)	1671, 1730, 1990, 2136
Bst2BI	CACGAG	1 cut(s)	1722
Bst2UI	CCWGG	2 cut(s)	611, 2405
Bst4CI	ACNGT	2 cut(s)	95, 417
Bst6I	CTCTTC	3 cut(s)	336, 1197, 2462
BstACI	GRCGYC	2 cut(s)	53, 1553
BstAFI	CTTAAG	1 cut(s)	2171
BstBAI	YACGTR	3 cut(s)	191, 529, 2009
BstBI	TTCGAA	2 cut(s)	386, 2024
BstC8I	GCNNGC	7 cut(s)	140, 607, 1074, 1298, 1863, 1910, 2017
BstDEI	CTNAG	7 cut(s)	240, 485, 550, 717, 1042, 1596, 1692
BstDSI	CCRYGG	5 cut(s)	129, 195, 1147, 1730, 2136
BstEII	GGTNACC	3 cut(s)	1666, 1754, 1994
BstF5I	GGATG	5 cut(s)	949, 1480, 1639, 1985, 2330
BstFNI	CGCG	3 cut(s)	131, 222, 1882
BstKTI	GATC	6 cut(s)	175, 477, 753, 990, 1791, 2185
BstMAI	GTCTC	4 cut(s)	245, 358, 560, 2331
BstMBI	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
BstMWI	GCNNNNNNNGC	9 cut(s)	79, 144, 153, 191, 713, 1130, 1709, 2288, 2327
BstNI	CCWGG	2 cut(s)	611, 2405
BstNSI	RCATGY	2 cut(s)	812, 1300
BstPI	GGTNACC	3 cut(s)	1666, 1754, 1994
BstSCI	CCNGG	6 cut(s)	609, 2037, 2220, 2221, 2250, 2403
BstSFI	CTRYAG	2 cut(s)	920, 1128
BstUI	CGCG	3 cut(s)	131, 222, 1882
BstV2I	GAAGAC	1 cut(s)	1214
BstX2I	RGATCY	1 cut(s)	172
BstYI	RGATCY	1 cut(s)	172
BsuI	GTATCC	3 cut(s)	1588, 1942, 2095
BsuRI	GGCC	3 cut(s)	1683, 2220, 2443
BtgI	CCRYGG	5 cut(s)	129, 195, 1147, 1730, 2136
BtgZI	GCGATG	1 cut(s)	320
BtsCI	GGATG	5 cut(s)	949, 1480, 1639, 1985, 2330
BtsI	GCAGTG	2 cut(s)	432, 1345
BtsIMutI	CAGTG	3 cut(s)	432, 1211, 1345
BveI	ACCTGC	1 cut(s)	2245
Cac8I	GCNNGC	7 cut(s)	140, 607, 1074, 1298, 1863, 1910, 2017
CaiI	CAGNNNCTG	3 cut(s)	1127, 1466, 1925
Cfr13I	GGNCC	3 cut(s)	1877, 2219, 2441
Cfr42I	CCGCGG	1 cut(s)	132
Cfr9I	CCCGGG	1 cut(s)	2221
CseI	GACGC	2 cut(s)	42, 746
Csp6I	GTAC	3 cut(s)	555, 972, 1443
CspCI	CAANNNNNGTGG	2 cut(s)	1129, 1164
CviQI	GTAC	3 cut(s)	555, 972, 1443
DdeI	CTNAG	7 cut(s)	240, 485, 550, 717, 1042, 1596, 1692
DpnI	GATC	6 cut(s)	174, 476, 752, 989, 1790, 2184
DpnII	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
Eam1104I	CTCTTC	3 cut(s)	336, 1197, 2462
EarI	CTCTTC	3 cut(s)	336, 1197, 2462
Ecl136II	GAGCTC	2 cut(s)	1931, 2161
Eco130I	CCWWGG	4 cut(s)	1671, 1730, 1990, 2136
Eco24I	GRGCYC	2 cut(s)	1933, 2163
Eco47I	GGWCC	1 cut(s)	1877
Eco53kI	GAGCTC	2 cut(s)	1931, 2161
Eco57I	CTGAAG	4 cut(s)	572, 2076, 2087, 2298
Eco72I	CACGTG	3 cut(s)	191, 529, 2009
Eco88I	CYCGRG	3 cut(s)	975, 2221, 2366
Eco91I	GGTNACC	3 cut(s)	1666, 1754, 1994
EcoICRI	GAGCTC	2 cut(s)	1931, 2161
EcoO65I	GGTNACC	3 cut(s)	1666, 1754, 1994
EcoRII	CCWGG	2 cut(s)	609, 2403
EcoT14I	CCWWGG	4 cut(s)	1671, 1730, 1990, 2136
EcoT22I	ATGCAT	1 cut(s)	748
EcoT38I	GRGCYC	2 cut(s)	1933, 2163
ErhI	CCWWGG	4 cut(s)	1671, 1730, 1990, 2136
Esp3I	CGTCTC	2 cut(s)	560, 2331
FalI	AAGNNNNNCTT	4 cut(s)	159, 191, 1310, 1342
FaqI	GGGAC	1 cut(s)	1268
FauI	CCCGC	1 cut(s)	2293
FauNDI	CATATG	1 cut(s)	685
FokI	GGATG	5 cut(s)	956, 1487, 1646, 1972, 2317
FriOI	GRGCYC	2 cut(s)	1933, 2163
FspBI	CTAG	8 cut(s)	650, 713, 1029, 1199, 1685, 1797, 2016, 2198
GsaI	CCCAGC	1 cut(s)	1137
GsuI	CTGGAG	1 cut(s)	1947
HaeIII	GGCC	3 cut(s)	1683, 2220, 2443
HapII	CCGG	3 cut(s)	2038, 2222, 2252
HgaI	GACGC	2 cut(s)	42, 746
Hin1I	GRCGYC	2 cut(s)	53, 1553
HincII	GTYRAC	2 cut(s)	1528, 2412
HindII	GTYRAC	2 cut(s)	1528, 2412
HindIII	AAGCTT	5 cut(s)	453, 547, 1070, 1415, 1859
HinfI	GANTC	6 cut(s)	100, 293, 1152, 1393, 2090, 2226
HpaI	GTTAAC	1 cut(s)	2412
HpaII	CCGG	3 cut(s)	2038, 2222, 2252
HphI	GGTGA	6 cut(s)	107, 1522, 1571, 1766, 1821, 1988
Hpy166II	GTNNAC	5 cut(s)	430, 1189, 1528, 1562, 2412
Hpy188III	TCNNGA	9 cut(s)	263, 461, 893, 932, 1085, 1241, 1318, 2334, 2395
Hpy8I	GTNNAC	5 cut(s)	430, 1189, 1528, 1562, 2412
Hpy99I	CGWCG	2 cut(s)	322, 762
HpyAV	CCTTC	2 cut(s)	695, 2062
HpyCH4III	ACNGT	2 cut(s)	95, 417
HpyCH4IV	ACGT	4 cut(s)	190, 528, 1553, 2008
HpyF10VI	GCNNNNNNNGC	9 cut(s)	79, 144, 153, 191, 713, 1130, 1709, 2288, 2327
HpyF3I	CTNAG	7 cut(s)	240, 485, 550, 717, 1042, 1596, 1692
HpySE526I	ACGT	4 cut(s)	190, 528, 1553, 2008
Hsp92I	GRCGYC	2 cut(s)	53, 1553
KspAI	GTTAAC	1 cut(s)	2412
KspI	CCGCGG	1 cut(s)	132
Kzo9I	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
LguI	GCTCTTC	2 cut(s)	336, 2462
LmnI	GCTCC	8 cut(s)	70, 628, 673, 704, 1129, 1323, 1928, 2158
LweI	GCATC	7 cut(s)	103, 592, 610, 1325, 1690, 1874, 2339
MaeI	CTAG	8 cut(s)	650, 713, 1029, 1199, 1685, 1797, 2016, 2198
MaeII	ACGT	4 cut(s)	190, 528, 1553, 2008
MaeIII	GTNAC	4 cut(s)	1517, 1666, 1754, 1994
MalI	GATC	6 cut(s)	174, 476, 752, 989, 1790, 2184
MboI	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
MfeI	CAATTG	1 cut(s)	2085
MflI	RGATCY	1 cut(s)	172
MhlI	GDGCHC	2 cut(s)	1933, 2163
MlyI	GAGTC	1 cut(s)	287
MmeI	TCCRAC	3 cut(s)	399, 702, 1602
Mph1103I	ATGCAT	1 cut(s)	748
MroXI	GAANNNNTTC	2 cut(s)	1156, 2061
MseI	TTAA	9 cut(s)	228, 282, 498, 1328, 1368, 1773, 2172, 2208, 2411
MslI	CAYNNNNRTG	2 cut(s)	1182, 1784
MspA1I	CMGCKG	5 cut(s)	131, 859, 1133, 2291, 2360
MspCI	CTTAAG	1 cut(s)	2171
MspI	CCGG	3 cut(s)	2038, 2222, 2252
MspR9I	CCNGG	6 cut(s)	611, 2039, 2222, 2223, 2252, 2405
MunI	CAATTG	1 cut(s)	2085
MvaI	CCWGG	2 cut(s)	611, 2405
MvnI	CGCG	3 cut(s)	131, 222, 1882
MwoI	GCNNNNNNNGC	9 cut(s)	79, 144, 153, 191, 713, 1130, 1709, 2288, 2327
NciI	CCSGG	4 cut(s)	2039, 2222, 2223, 2252
NcoI	CCATGG	2 cut(s)	1730, 2136
NdeI	CATATG	1 cut(s)	685
NdeII	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
NheI	GCTAGC	1 cut(s)	2015
NlaIV	GGNNCC	3 cut(s)	72, 630, 2402
NmeAIII	GCCGAG	1 cut(s)	1511
NmuCI	GTSAC	2 cut(s)	1754, 1994
NsiI	ATGCAT	1 cut(s)	748
NspI	RCATGY	2 cut(s)	812, 1300
NspV	TTCGAA	2 cut(s)	386, 2024
PaeI	GCATGC	1 cut(s)	1300
PaeR7I	CTCGAG	1 cut(s)	975
PciSI	GCTCTTC	2 cut(s)	336, 2462
PdmI	GAANNNNTTC	2 cut(s)	1156, 2061
PfeI	GAWTC	5 cut(s)	100, 1152, 1393, 2090, 2226
PflMI	CCANNNNNTGG	1 cut(s)	1991
PleI	GAGTC	1 cut(s)	287
PmaCI	CACGTG	3 cut(s)	191, 529, 2009
PmlI	CACGTG	3 cut(s)	191, 529, 2009
PpsI	GAGTC	1 cut(s)	287
Ppu21I	YACGTR	3 cut(s)	191, 529, 2009
PsiI	TTATAA	2 cut(s)	1313, 1652
Psp124BI	GAGCTC	2 cut(s)	1933, 2163
Psp6I	CCWGG	2 cut(s)	609, 2403
PspCI	CACGTG	3 cut(s)	191, 529, 2009
PspEI	GGTNACC	3 cut(s)	1666, 1754, 1994
PspFI	CCCAGC	1 cut(s)	1133
PspGI	CCWGG	2 cut(s)	609, 2403
PspN4I	GGNNCC	3 cut(s)	72, 630, 2402
PspPI	GGNCC	3 cut(s)	1877, 2219, 2441
PspXI	VCTCGAGB	1 cut(s)	975
PstI	CTGCAG	1 cut(s)	1132
PstNI	CAGNNNCTG	3 cut(s)	1127, 1466, 1925
PsuI	RGATCY	1 cut(s)	172
PvuII	CAGCTG	4 cut(s)	859, 1133, 2291, 2360
RsaI	GTAC	3 cut(s)	556, 973, 1444
RsaNI	GTAC	3 cut(s)	555, 972, 1443
RseI	CAYNNNNRTG	2 cut(s)	1182, 1784
SacI	GAGCTC	2 cut(s)	1933, 2163
SacII	CCGCGG	1 cut(s)	132
SapI	GCTCTTC	2 cut(s)	336, 2462
SaqAI	TTAA	9 cut(s)	228, 282, 498, 1328, 1368, 1773, 2172, 2208, 2411
Sau3AI	GATC	6 cut(s)	172, 474, 750, 987, 1788, 2182
Sau96I	GGNCC	3 cut(s)	1877, 2219, 2441
SchI	GAGTC	1 cut(s)	287
ScrFI	CCNGG	6 cut(s)	611, 2039, 2222, 2223, 2252, 2405
SduI	GDGCHC	2 cut(s)	1933, 2163
SfaNI	GCATC	7 cut(s)	103, 592, 610, 1325, 1690, 1874, 2339
SfcI	CTRYAG	2 cut(s)	920, 1128
Sfr274I	CTCGAG	1 cut(s)	975
Sfr303I	CCGCGG	1 cut(s)	132
SfuI	TTCGAA	2 cut(s)	386, 2024
SgrBI	CCGCGG	1 cut(s)	132
SinI	GGWCC	1 cut(s)	1877
SlaI	CTCGAG	1 cut(s)	975
SmaI	CCCGGG	1 cut(s)	2223
SmiMI	CAYNNNNRTG	2 cut(s)	1182, 1784
SmlI	CTYRAG	5 cut(s)	544, 975, 1953, 2162, 2171
SmoI	CTYRAG	5 cut(s)	544, 975, 1953, 2162, 2171
SpeI	ACTAGT	1 cut(s)	649
SphI	GCATGC	1 cut(s)	1300
SspMI	CTAG	8 cut(s)	650, 713, 1029, 1199, 1685, 1797, 2016, 2198
SstI	GAGCTC	2 cut(s)	1933, 2163
StyD4I	CCNGG	6 cut(s)	609, 2037, 2220, 2221, 2250, 2403
StyI	CCWWGG	4 cut(s)	1671, 1730, 1990, 2136
TaaI	ACNGT	2 cut(s)	95, 417
TaiI	ACGT	4 cut(s)	193, 531, 1556, 2011
TaqI	TCGA	3 cut(s)	386, 976, 2024
TauI	GCSGC	5 cut(s)	76, 131, 185, 413, 2321
TfiI	GAWTC	5 cut(s)	100, 1152, 1393, 2090, 2226
Tru1I	TTAA	9 cut(s)	228, 282, 498, 1328, 1368, 1773, 2172, 2208, 2411
Tru9I	TTAA	9 cut(s)	228, 282, 498, 1328, 1368, 1773, 2172, 2208, 2411
TscAI	CASTG	3 cut(s)	432, 1218, 1345
TseFI	GTSAC	2 cut(s)	1754, 1994
Tsp45I	GTSAC	2 cut(s)	1754, 1994
TspGWI	ACGGA	2 cut(s)	584, 1136
TspMI	CCCGGG	1 cut(s)	2221
TspRI	CASTG	3 cut(s)	432, 1218, 1345
Van91I	CCANNNNNTGG	1 cut(s)	1991
Vha464I	CTTAAG	1 cut(s)	2171
VpaK11BI	GGWCC	1 cut(s)	1877
XceI	RCATGY	2 cut(s)	812, 1300
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1553
XhoI	CTCGAG	1 cut(s)	975
XmaI	CCCGGG	1 cut(s)	2221
XmnI	GAANNNNTTC	2 cut(s)	1156, 2061
XspI	CTAG	8 cut(s)	650, 713, 1029, 1199, 1685, 1797, 2016, 2198
ZraI	GACGTC	1 cut(s)	1554
Zsp2I	ATGCAT	1 cut(s)	748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.