Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0010294

Physical Location & Seq

Genomic Coordinates Forward (+)

68906408 .. 68915754

Gene Structure 9347 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020801

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGGACAAGTCCAGCGCTCTCGAATACATCAACCAGATGTTCCCCACAGAGGCATCGTTATCCGGCGTCGAGCCGCTAATGCAGAAGATACACAGTGAAATTCGGCGCGTTGATGCTGGAATTCTAGCTGCCGTTCGTCAGCAGAGCAATTCAGGGACCAAAGCCAAGGAAGATCTTGCTGCCGCTACCCGAGCTGTGGAGGTCTTCTCTGCTGTTGAACAGCTTCAAGTGATGGCTTCCAAACGACAATATAAAGAGGCGGCTGCACAGTTGGAGCTCAGGGAGAAATTTAAGAATATCAAACAAATATTAAAGTCGCACGTGTTTTCTGATTTCTCAAGCTTAGGTACTGGAAAGGAGTCGGAAGAATCTAATTTACTCCAGCAGCTATCTGAGGCTTGCTTAGTTGTTGATGCATTAGAGGCATCTGTGAGGGAAGAATTAGTAAATAACTTTTGCAGCAGGGAGCTTACTTCTTATGAGCAAATTTTTGAAGGAGCTGAACTAGCTAAGTTGGATAAGACTGAACGAAGATATGCATGGATCAAGCGCCGCATGAGAACGAATGAAGAAATCTGGAAAATCTTTCCTTCTTCATGGCATGTTTCTTATCGACTCTGCATTCAGTTCTGCAAAAAAACAAGGAAGCAGCTAGAAGATATCCTTAATAATCAGAAAGAAAAGCCAGATGTTGGGACATTGCTGATGGCACTACAGCGAACTCTTGAATTTGAGGATGAATTGGCAGAAAAATTTGGAGGTGGTACTCGAGGCAGGGAGGTTGGAAATGAGATTGAAGAAATTGGTAGAGGAGAAAACACTACTCAAAGTGCTTCAGATATTCGAAAGAAATATGAAAAGAAGCTTGCTGCCCATCAAGGAAATGCCACAGAGGAAAAGGATAAAGAGTTATCTGTGCCTGGAGCTGGGTTCAACTTCCGTGGAATCATCTCATCTTGCTTCGAGCCTCACTTGACTGTTTACACAGAACTAGAAGAGAAGACACTAATGGAGAATTTGGAAAAACTTGTTCAGGAGGAGACATGGGATGTTGAAGAGGGAAGTCAGAATAATGTTTTGTCAAGTAGCATGCAGTTGTTTCTTATAATCAAGAGGAGCTTAAAACGGTGCAGTGCTTTAACAAAGAATCAGACATTATTTAACTTGTTCAAGGTGTTTCAAAGGATTCTCAAAGCATATGCAACAAAGCTTTTTATAAGATTACCTAAAGGCGGTACAGGAATTGTTGCAGCAGCAACTGGTATGGATGGACAGATAAAGACATCAGACAGAGATGAGAGGGTGATTTGTTACATTGTCAATTCAGCGGAATATTGCACCAACACGTCTGGTGAACTCGCTGAGAGTGTGTCCAAAATAATTGATTCTCAATTCGCAGATGGGGTGGATATGTCGGAAGTGCAGGATGAATTTTCAGCAGTGATAACAAGAGCATTGGTAACCTTGGTGCATGGCCTAGAAACCAAGTTTGACAATGAAATGGCTGCAATGACACGAGTTCCATGGGGTACACTTGAAAGTGTTGGTGACCAATCAGAGTATGTTAATGGCATAAATATGATCCTTGCTAGTAGTATACCTATACTTGGAAGCCTTCTTTCACCGATCTACTTCCAATTCTTCTTGGACAAGCTGGCATCATCTCTTGGTCCACGTTTCTATGCTAACATTTTCAAGTGTAAGCAAATATCAGAGACTGGAGCTCAACAGATGCTATTGGATACTCAAGCTGTAAAGACTATTCTTTTGGATATCCCATCACTTGGTCACCAGACGTCACGTGCTGCAAGCTATGCGAAATTTGTAAGCCGTGAGATGAGCAAAGCTGAAGCACTTCTAAAGGTCATACTGTCCCCAATTGATTCTGTCGCAGACACATATCGTGCATTGCTACCAGAGGGAACACCTATGGAGTTTCAGCGAATTCTGGAGCTCAAGGGTCTTAAAAAGGCAGATCAGCAGAGTATACTTGAAGACTTCAACAAGCATGGGCCGGGAATTACTCAACCTTCTACCCCACCACCACCAGTTGCCGCACCTGTTCCAACTGCCCCGACAGTCTCTCTGATACAAATTCCTACTTCAGGTGGATTCCTTGCACCCAGAGAAGATGTTCTAACCAGAGCAGCTGCACTTGGACGTGGTGCAGCCACAACAGGGTTCAAGAGGTTTCTAGCTTTAACAGAAGCTGCAAAGGACCGGAAAGATGGGCCTTTCAGGAAGCTCTTCAATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000938 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005794 GO:0006810 GO:0006890 GO:0006950 GO:0006970 GO:0007009 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0010256 GO:0010286 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032991 GO:0042147 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps53_N

Protein Analysis

753

Amino Acids

83.64

Weight (kDa)

6.11

Isoelectric Point (pI)

39.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps53_N	PF04100	3 - 79	1.3e-16	Vps53, N-terminal
Vps53_N	PF04100	77 - 347	2.2e-75	Vps53, N-terminal
VPS53_C	PF16854	577 - 655	8.4e-14	Vacuolar protein sorting-associated protein 53 C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50500 AT1G50500 AT1G50500 AT1G50500 AT1G50500
fragaria_vesca	FvH4_6g40130 FvH4_6g40130
malus_domestica	MD09G1135000.v1.1 MD17G1124000.v1.1
prunus_persica	Prupe.3G191700_v2.0.a1
pyrus_communis	pycom09g05610 pycom17g11540
rosa_chinensis	RchiOBHm_Chr2g0154761
rosa_laevigata	RLG00000020801
rosa_multiflora	Rmu_sc0001027.1_g000002
rosa_roxburghii	Rroxscaffold_2G00094120
rosa_rugosa	Rorug02G0444800
rosa_samantha	Rh2BG521400 Rh2CG495700 Rh2DG531500
rosa_wichuraiana	Rw2G041990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1106, 1217
AatII	GACGTC	1 cut(s)	1799
AccB7I	CCANNNNNTGG	2 cut(s)	692, 1784
AccI	GTMKAC	2 cut(s)	1597, 1987
AccII	CGCG	1 cut(s)	108
AciI	CCGC	7 cut(s)	74, 183, 260, 553, 1233, 1328, 2055
AclWI	GGATC	2 cut(s)	551, 1576
AcuI	CTGAAG	3 cut(s)	819, 1869, 2088
AcvI	CACGTG	2 cut(s)	322, 1802
AcyI	GRCGYC	2 cut(s)	66, 1796
AfaI	GTAC	4 cut(s)	349, 766, 1237, 1531
AfeI	AGCGCT	1 cut(s)	16
AfiI	CCNNNNNNNGG	9 cut(s)	49, 196, 692, 926, 1232, 1607, 1784, 2105, 2178
AflIII	ACRYGT	2 cut(s)	321, 1344
AjiI	CACGTC	2 cut(s)	1347, 2162
AjnI	CCWGG	1 cut(s)	919
Alw21I	GWGCWC	3 cut(s)	279, 1726, 1956
Alw26I	GTCTC	3 cut(s)	1034, 1709, 2086
AlwI	GGATC	2 cut(s)	551, 1576
AlwNI	CAGNNNCTG	3 cut(s)	1259, 1718, 2210
Ama87I	CYCGRG	2 cut(s)	189, 768
Aor51HI	AGCGCT	1 cut(s)	16
AoxI	GGCC	3 cut(s)	1474, 2012, 2231
ArsI	GACNNNNNNTTYG	2 cut(s)	1750, 1782
Asp700I	GAANNNNTTC	4 cut(s)	222, 1854, 2133, 2246
AspLEI	GCGC	3 cut(s)	17, 108, 552
AspS9I	GGNCC	5 cut(s)	156, 1670, 2012, 2218, 2231
AsuC2I	CCSGG	1 cut(s)	2016
AsuHPI	GGTGA	5 cut(s)	1315, 1364, 1559, 1614, 1781
AsuII	TTCGAA	1 cut(s)	844
AvaI	CYCGRG	2 cut(s)	189, 768
AvaII	GGWCC	3 cut(s)	156, 1670, 2218
BanII	GRGCYC	3 cut(s)	279, 1726, 1956
BauI	CACGAG	1 cut(s)	1515
BbrPI	CACGTG	2 cut(s)	322, 1802
BbsI	GAAGAC	3 cut(s)	196, 1007, 2001
Bbv12I	GWGCWC	3 cut(s)	279, 1726, 1956
BccI	CCATC	7 cut(s)	226, 700, 882, 1262, 1394, 1786, 2222
BceAI	ACGGC	2 cut(s)	116, 1815
BciT130I	CCWGG	1 cut(s)	921
BciVI	GTATCC	1 cut(s)	1735
BcnI	CCSGG	1 cut(s)	2016
BcoDI	GTCTC	3 cut(s)	1034, 1709, 2086
BfaI	CTAG	7 cut(s)	125, 506, 653, 992, 1478, 1590, 2195
BfmI	CTRYAG	1 cut(s)	713
BfoI	RGCGCY	2 cut(s)	18, 553
BfuI	GTATCC	1 cut(s)	1735
BglII	AGATCT	1 cut(s)	172
Bme1390I	CCNGG	2 cut(s)	921, 2016
Bme18I	GGWCC	3 cut(s)	156, 1670, 2218
BmeT110I	CYCGRG	2 cut(s)	189, 768
BmgBI	CACGTC	2 cut(s)	1347, 2162
BmgT120I	GGNCC	5 cut(s)	156, 1670, 2012, 2218, 2231
BmiI	GGNNCC	1 cut(s)	157
BmrFI	CCNGG	2 cut(s)	921, 2016
BmsI	GCATC	6 cut(s)	62, 103, 403, 434, 1667, 1722
BpiI	GAAGAC	3 cut(s)	196, 1007, 2001
BplI	GAGNNNNNCTC	2 cut(s)	191, 223
BpmI	CTGGAG	4 cut(s)	365, 942, 1740, 1970
Bpu10I	CCTNAGC	2 cut(s)	278, 343
Bpu14I	TTCGAA	1 cut(s)	844
BpuEI	CTTGAG	3 cut(s)	322, 1731, 1940
BpuMI	CCSGG	1 cut(s)	2016
BsaAI	YACGTR	2 cut(s)	322, 1802
BsaHI	GRCGYC	2 cut(s)	66, 1796
BsaJI	CCNNGG	4 cut(s)	165, 940, 1464, 1523
BsaWI	WCCGGW	1 cut(s)	2220
Bsc4I	CCNNNNNNNGG	9 cut(s)	49, 196, 692, 926, 1232, 1607, 1784, 2105, 2178
Bse1I	ACTGG	4 cut(s)	355, 1264, 1723, 2048
Bse3DI	GCAATG	3 cut(s)	698, 1515, 1907
BseBI	CCWGG	1 cut(s)	921
BseDI	CCNNGG	4 cut(s)	165, 940, 1464, 1523
BseGI	GGATG	4 cut(s)	742, 1054, 1273, 1432
BseLI	CCNNNNNNNGG	9 cut(s)	49, 196, 692, 926, 1232, 1607, 1784, 2105, 2178
BseMI	GCAATG	3 cut(s)	698, 1515, 1907
BseMII	CTCAG	3 cut(s)	292, 384, 1353
BseNI	ACTGG	4 cut(s)	355, 1264, 1723, 2048
BseRI	GAGGAG	3 cut(s)	825, 1052, 1129
BseYI	CCCAGC	1 cut(s)	926
BsgI	GTGCAG	5 cut(s)	249, 1150, 1442, 2136, 2187
Bsh1236I	CGCG	1 cut(s)	108
BshFI	GGCC	3 cut(s)	1476, 2014, 2233
BsiHKAI	GWGCWC	3 cut(s)	279, 1726, 1956
BsiHKCI	CYCGRG	2 cut(s)	189, 768
BsiSI	CCGG	3 cut(s)	63, 2015, 2221
BslFI	GGGAC	3 cut(s)	169, 709, 1858
BslI	CCNNNNNNNGG	9 cut(s)	49, 196, 692, 926, 1232, 1607, 1784, 2105, 2178
BsmAI	GTCTC	3 cut(s)	1034, 1709, 2086
BsmFI	GGGAC	3 cut(s)	169, 709, 1858
BsmI	GAATGC	1 cut(s)	621
BsnI	GGCC	3 cut(s)	1476, 2014, 2233
BsoBI	CYCGRG	2 cut(s)	189, 768
Bsp119I	TTCGAA	1 cut(s)	844
Bsp1286I	GDGCHC	3 cut(s)	279, 1726, 1956
Bsp143I	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
Bsp19I	CCATGG	1 cut(s)	1523
BspACI	CCGC	7 cut(s)	74, 183, 260, 553, 1233, 1328, 2055
BspANI	GGCC	3 cut(s)	1476, 2014, 2233
BspCNI	CTCAG	3 cut(s)	291, 385, 1354
BspFNI	CGCG	1 cut(s)	108
BspLI	GGNNCC	1 cut(s)	157
BspPI	GGATC	2 cut(s)	551, 1576
BspQI	GCTCTTC	1 cut(s)	2252
BspT104I	TTCGAA	1 cut(s)	844
BsrDI	GCAATG	3 cut(s)	698, 1515, 1907
BsrI	ACTGG	4 cut(s)	355, 1264, 1723, 2048
BssECI	CCNNGG	4 cut(s)	165, 940, 1464, 1523
BssMI	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
BssNAI	GTATAC	2 cut(s)	1598, 1988
BssNI	GRCGYC	2 cut(s)	66, 1796
BssSI	CACGAG	1 cut(s)	1515
BssT1I	CCWWGG	3 cut(s)	165, 1464, 1523
Bst1107I	GTATAC	2 cut(s)	1598, 1988
Bst2BI	CACGAG	1 cut(s)	1515
Bst2UI	CCWGG	1 cut(s)	921
Bst4CI	ACNGT	6 cut(s)	95, 270, 979, 1128, 1872, 2080
Bst6I	CTCTTC	3 cut(s)	990, 1050, 2252
BstACI	GRCGYC	2 cut(s)	66, 1796
BstAPI	GCANNNNNTGC	1 cut(s)	1814
BstBAI	YACGTR	2 cut(s)	322, 1802
BstBI	TTCGAA	1 cut(s)	844
BstC8I	GCNNGC	5 cut(s)	400, 867, 1091, 1656, 1810
BstDEI	CTNAG	6 cut(s)	278, 343, 393, 403, 510, 1362
BstDSI	CCRYGG	2 cut(s)	940, 1523
BstEII	GGTNACC	3 cut(s)	1459, 1547, 1787
BstENI	CCTNNNNNAGG	1 cut(s)	2103
BstF5I	GGATG	4 cut(s)	742, 1054, 1273, 1432
BstFNI	CGCG	1 cut(s)	108
BstH2I	RGCGCY	2 cut(s)	18, 553
BstHHI	GCGC	3 cut(s)	17, 108, 552
BstKTI	GATC	5 cut(s)	175, 546, 1584, 1629, 1978
BstMAI	GTCTC	3 cut(s)	1034, 1709, 2086
BstMBI	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
BstMWI	GCNNNNNNNGC	5 cut(s)	79, 191, 422, 506, 1814
BstNI	CCWGG	1 cut(s)	921
BstNSI	RCATGY	2 cut(s)	605, 1093
BstPI	GGTNACC	3 cut(s)	1459, 1547, 1787
BstSCI	CCNGG	2 cut(s)	919, 2014
BstSFI	CTRYAG	1 cut(s)	713
BstUI	CGCG	1 cut(s)	108
BstV2I	GAAGAC	3 cut(s)	196, 1007, 2001
BstX2I	RGATCY	1 cut(s)	172
BstYI	RGATCY	1 cut(s)	172
BstZ17I	GTATAC	2 cut(s)	1598, 1988
BsuI	GTATCC	1 cut(s)	1735
BsuRI	GGCC	3 cut(s)	1476, 2014, 2233
BtgI	CCRYGG	2 cut(s)	940, 1523
BtrI	CACGTC	2 cut(s)	1347, 2162
BtsCI	GGATG	4 cut(s)	742, 1054, 1273, 1432
BtsI	GCAGTG	2 cut(s)	1138, 1446
BtsIMutI	CAGTG	3 cut(s)	100, 1138, 1446
Cac8I	GCNNGC	5 cut(s)	400, 867, 1091, 1656, 1810
CaiI	CAGNNNCTG	3 cut(s)	1259, 1718, 2210
CfoI	GCGC	3 cut(s)	17, 108, 552
Cfr13I	GGNCC	5 cut(s)	156, 1670, 2012, 2218, 2231
CseI	GACGC	1 cut(s)	55
Csp6I	GTAC	4 cut(s)	348, 765, 1236, 1530
CspCI	CAANNNNNGTGG	2 cut(s)	922, 957
CviAII	CATG	9 cut(s)	540, 556, 597, 602, 1044, 1090, 1472, 1524, 2009
CviQI	GTAC	4 cut(s)	348, 765, 1236, 1530
DdeI	CTNAG	6 cut(s)	278, 343, 393, 403, 510, 1362
DpnI	GATC	5 cut(s)	174, 545, 1583, 1628, 1977
DpnII	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
Eam1104I	CTCTTC	3 cut(s)	990, 1050, 2252
EarI	CTCTTC	3 cut(s)	990, 1050, 2252
Ecl136II	GAGCTC	3 cut(s)	277, 1724, 1954
Eco130I	CCWWGG	3 cut(s)	165, 1464, 1523
Eco24I	GRGCYC	3 cut(s)	279, 1726, 1956
Eco32I	GATATC	2 cut(s)	661, 1774
Eco47I	GGWCC	3 cut(s)	156, 1670, 2218
Eco47III	AGCGCT	1 cut(s)	16
Eco53kI	GAGCTC	3 cut(s)	277, 1724, 1954
Eco57I	CTGAAG	3 cut(s)	819, 1869, 2088
Eco72I	CACGTG	2 cut(s)	322, 1802
Eco88I	CYCGRG	2 cut(s)	189, 768
Eco91I	GGTNACC	3 cut(s)	1459, 1547, 1787
EcoICRI	GAGCTC	3 cut(s)	277, 1724, 1954
EcoNI	CCTNNNNNAGG	1 cut(s)	2103
EcoO65I	GGTNACC	3 cut(s)	1459, 1547, 1787
EcoRI	GAATTC	2 cut(s)	120, 1944
EcoRII	CCWGG	1 cut(s)	919
EcoRV	GATATC	2 cut(s)	661, 1774
EcoT14I	CCWWGG	3 cut(s)	165, 1464, 1523
EcoT22I	ATGCAT	2 cut(s)	418, 541
EcoT38I	GRGCYC	3 cut(s)	279, 1726, 1956
ErhI	CCWWGG	3 cut(s)	165, 1464, 1523
FaeI	CATG	9 cut(s)	543, 559, 600, 605, 1047, 1093, 1475, 1527, 2012
FalI	AAGNNNNNCTT	4 cut(s)	648, 680, 1103, 1135
FaqI	GGGAC	3 cut(s)	169, 709, 1858
FatI	CATG	9 cut(s)	539, 555, 596, 601, 1043, 1089, 1471, 1523, 2008
FauNDI	CATATG	1 cut(s)	1198
FblI	GTMKAC	2 cut(s)	1597, 1987
FokI	GGATG	4 cut(s)	749, 1061, 1280, 1439
FriOI	GRGCYC	3 cut(s)	279, 1726, 1956
FspBI	CTAG	7 cut(s)	125, 506, 653, 992, 1478, 1590, 2195
GlaI	GCGC	3 cut(s)	16, 107, 551
GsaI	CCCAGC	1 cut(s)	930
GsuI	CTGGAG	4 cut(s)	365, 942, 1740, 1970
HaeII	RGCGCY	2 cut(s)	18, 553
HaeIII	GGCC	3 cut(s)	1476, 2014, 2233
HapII	CCGG	3 cut(s)	63, 2015, 2221
HgaI	GACGC	1 cut(s)	55
HhaI	GCGC	3 cut(s)	17, 108, 552
Hin1I	GRCGYC	2 cut(s)	66, 1796
Hin1II	CATG	9 cut(s)	543, 559, 600, 605, 1047, 1093, 1475, 1527, 2012
Hin6I	GCGC	3 cut(s)	15, 106, 550
HinP1I	GCGC	3 cut(s)	15, 106, 550
HindIII	AAGCTT	3 cut(s)	340, 863, 1208
HinfI	GANTC	9 cut(s)	359, 368, 615, 945, 1147, 1186, 1385, 1883, 2112
HpaII	CCGG	3 cut(s)	63, 2015, 2221
HphI	GGTGA	5 cut(s)	1315, 1364, 1559, 1614, 1781
Hpy166II	GTNNAC	6 cut(s)	982, 1355, 1532, 1598, 1673, 1988
Hpy188III	TCNNGA	8 cut(s)	20, 577, 725, 1034, 1111, 1949, 2185, 2239
Hpy8I	GTNNAC	6 cut(s)	982, 1355, 1532, 1598, 1673, 1988
Hpy99I	CGWCG	1 cut(s)	71
HpyAV	CCTTC	4 cut(s)	488, 600, 1625, 2040
HpyCH4III	ACNGT	6 cut(s)	95, 270, 979, 1128, 1872, 2080
HpyCH4IV	ACGT	6 cut(s)	321, 1346, 1675, 1796, 1801, 2161
HpyF10VI	GCNNNNNNNGC	5 cut(s)	79, 191, 422, 506, 1814
HpyF3I	CTNAG	6 cut(s)	278, 343, 393, 403, 510, 1362
HpySE526I	ACGT	6 cut(s)	321, 1346, 1675, 1796, 1801, 2161
Hsp92I	GRCGYC	2 cut(s)	66, 1796
Hsp92II	CATG	9 cut(s)	543, 559, 600, 605, 1047, 1093, 1475, 1527, 2012
HspAI	GCGC	3 cut(s)	15, 106, 550
Kzo9I	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
LguI	GCTCTTC	1 cut(s)	2252
LmnI	GCTCC	7 cut(s)	274, 466, 497, 923, 1116, 1721, 1951
LweI	GCATC	6 cut(s)	62, 103, 403, 434, 1667, 1722
MaeI	CTAG	7 cut(s)	125, 506, 653, 992, 1478, 1590, 2195
MaeII	ACGT	6 cut(s)	321, 1346, 1675, 1796, 1801, 2161
MaeIII	GTNAC	5 cut(s)	1310, 1459, 1547, 1787, 1797
MalI	GATC	5 cut(s)	174, 545, 1583, 1628, 1977
MboI	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
MfeI	CAATTG	1 cut(s)	1878
MflI	RGATCY	1 cut(s)	172
MhlI	GDGCHC	3 cut(s)	279, 1726, 1956
MlyI	GAGTC	2 cut(s)	368, 609
MmeI	TCCRAC	6 cut(s)	252, 342, 495, 763, 1395, 2090
Mph1103I	ATGCAT	2 cut(s)	418, 541
MroXI	GAANNNNTTC	4 cut(s)	222, 1854, 2133, 2246
MseI	TTAA	9 cut(s)	291, 311, 666, 1121, 1139, 1161, 1566, 1965, 2201
MslI	CAYNNNNRTG	1 cut(s)	1577
MspA1I	CMGCKG	2 cut(s)	1328, 2150
MspI	CCGG	3 cut(s)	63, 2015, 2221
MspR9I	CCNGG	2 cut(s)	921, 2016
MunI	CAATTG	1 cut(s)	1878
Mva1269I	GAATGC	1 cut(s)	621
MvaI	CCWGG	1 cut(s)	921
MvnI	CGCG	1 cut(s)	108
MwoI	GCNNNNNNNGC	5 cut(s)	79, 191, 422, 506, 1814
NciI	CCSGG	1 cut(s)	2016
NcoI	CCATGG	1 cut(s)	1523
NdeI	CATATG	1 cut(s)	1198
NdeII	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
NlaIII	CATG	9 cut(s)	543, 559, 600, 605, 1047, 1093, 1475, 1527, 2012
NlaIV	GGNNCC	1 cut(s)	157
NmuCI	GTSAC	3 cut(s)	1547, 1787, 1797
NsiI	ATGCAT	2 cut(s)	418, 541
NspI	RCATGY	2 cut(s)	605, 1093
NspV	TTCGAA	1 cut(s)	844
PaeI	GCATGC	1 cut(s)	1093
PaeR7I	CTCGAG	1 cut(s)	768
PciSI	GCTCTTC	1 cut(s)	2252
PctI	GAATGC	1 cut(s)	621
PdmI	GAANNNNTTC	4 cut(s)	222, 1854, 2133, 2246
PfeI	GAWTC	7 cut(s)	368, 945, 1147, 1186, 1385, 1883, 2112
PflMI	CCANNNNNTGG	2 cut(s)	692, 1784
PleI	GAGTC	2 cut(s)	367, 609
PmaCI	CACGTG	2 cut(s)	322, 1802
PmlI	CACGTG	2 cut(s)	322, 1802
PpsI	GAGTC	2 cut(s)	367, 609
Ppu21I	YACGTR	2 cut(s)	322, 1802
PsiI	TTATAA	2 cut(s)	1106, 1217
Psp124BI	GAGCTC	3 cut(s)	279, 1726, 1956
Psp6I	CCWGG	1 cut(s)	919
PspCI	CACGTG	2 cut(s)	322, 1802
PspEI	GGTNACC	3 cut(s)	1459, 1547, 1787
PspFI	CCCAGC	1 cut(s)	926
PspGI	CCWGG	1 cut(s)	919
PspN4I	GGNNCC	1 cut(s)	157
PspPI	GGNCC	5 cut(s)	156, 1670, 2012, 2218, 2231
PspXI	VCTCGAGB	1 cut(s)	768
PstNI	CAGNNNCTG	3 cut(s)	1259, 1718, 2210
PsuI	RGATCY	1 cut(s)	172
PvuII	CAGCTG	1 cut(s)	2150
RsaI	GTAC	4 cut(s)	349, 766, 1237, 1531
RsaNI	GTAC	4 cut(s)	348, 765, 1236, 1530
RseI	CAYNNNNRTG	1 cut(s)	1577
SacI	GAGCTC	3 cut(s)	279, 1726, 1956
SapI	GCTCTTC	1 cut(s)	2252
SaqAI	TTAA	9 cut(s)	291, 311, 666, 1121, 1139, 1161, 1566, 1965, 2201
Sau3AI	GATC	5 cut(s)	172, 543, 1581, 1626, 1975
Sau96I	GGNCC	5 cut(s)	156, 1670, 2012, 2218, 2231
SchI	GAGTC	2 cut(s)	368, 609
ScrFI	CCNGG	2 cut(s)	921, 2016
SduI	GDGCHC	3 cut(s)	279, 1726, 1956
SfaNI	GCATC	6 cut(s)	62, 103, 403, 434, 1667, 1722
SfcI	CTRYAG	1 cut(s)	713
Sfr274I	CTCGAG	1 cut(s)	768
SfuI	TTCGAA	1 cut(s)	844
SinI	GGWCC	3 cut(s)	156, 1670, 2218
SlaI	CTCGAG	1 cut(s)	768
SmiMI	CAYNNNNRTG	1 cut(s)	1577
SmlI	CTYRAG	4 cut(s)	337, 768, 1746, 1955
SmoI	CTYRAG	4 cut(s)	337, 768, 1746, 1955
SphI	GCATGC	1 cut(s)	1093
SsiI	CCGC	7 cut(s)	74, 183, 260, 553, 1233, 1328, 2055
SspI	AATATT	2 cut(s)	309, 1334
SspMI	CTAG	7 cut(s)	125, 506, 653, 992, 1478, 1590, 2195
SstI	GAGCTC	3 cut(s)	279, 1726, 1956
StyD4I	CCNGG	2 cut(s)	919, 2014
StyI	CCWWGG	3 cut(s)	165, 1464, 1523
TaaI	ACNGT	6 cut(s)	95, 270, 979, 1128, 1872, 2080
TaiI	ACGT	6 cut(s)	324, 1349, 1678, 1799, 1804, 2164
TaqI	TCGA	6 cut(s)	21, 69, 613, 769, 844, 963
TauI	GCSGC	5 cut(s)	76, 185, 263, 555, 2057
TfiI	GAWTC	7 cut(s)	368, 945, 1147, 1186, 1385, 1883, 2112
Tru1I	TTAA	9 cut(s)	291, 311, 666, 1121, 1139, 1161, 1566, 1965, 2201
Tru9I	TTAA	9 cut(s)	291, 311, 666, 1121, 1139, 1161, 1566, 1965, 2201
TscAI	CASTG	3 cut(s)	100, 1138, 1446
TseFI	GTSAC	3 cut(s)	1547, 1787, 1797
Tsp45I	GTSAC	3 cut(s)	1547, 1787, 1797
TspDTI	ATGAA	6 cut(s)	582, 585, 753, 870, 1443, 1512
TspGWI	ACGGA	1 cut(s)	929
TspRI	CASTG	3 cut(s)	100, 1138, 1446
Van91I	CCANNNNNTGG	2 cut(s)	692, 1784
VpaK11BI	GGWCC	3 cut(s)	156, 1670, 2218
XagI	CCTNNNNNAGG	1 cut(s)	2103
XceI	RCATGY	2 cut(s)	605, 1093
XhoI	CTCGAG	1 cut(s)	768
XmiI	GTMKAC	2 cut(s)	1597, 1987
XmnI	GAANNNNTTC	4 cut(s)	222, 1854, 2133, 2246
XspI	CTAG	7 cut(s)	125, 506, 653, 992, 1478, 1590, 2195
ZraI	GACGTC	1 cut(s)	1797
Zsp2I	ATGCAT	2 cut(s)	418, 541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.